Association between three glycosidases activity A - mannosidase ( A-ΜΑΝ ), B-Ν-acetyloglucosaminidase (NAGASE) and B - galactosidase (B-GAL) andin vitro fertilization of bovine oocytes collected from different-sized follicles

We studied the role of three glycosidases (α-mannosidase - α-ΜΑΝ, β-N-acetyloglucosaminidase -NAGASE and β-galactosidase - β-GAL) in follicularfluid (FF) and in fertilization medium (FM) of bovineoocytes. Oocytes were allocated into 3 groups accordingto the follicular size (controls - CF: 2-8 mm, smallfollicle group - SF: 2-5 mm, large follicle group - LF:>5-8 mm). Bovine embryos were produced in vitroeither in groups (experiment 1, n = 2099 oocytes) orindividually (experiment 2, n = 79 oocytes). In bothexperiments, the activity of all glycosidases in the FF oflarge follicles was significantly lower than in the FF ofsmall follicles group. In the FM of LF-group oocytes, α-MAN and NAGASE were significantly higher comparedto SF- and CF-group oocytes (experiment 1) and β-GALwas significantly higher in SF- compared to CF-groupoocytes (experiment 2). Cleavage rate was similar amongall groups in both experiments; however significantlyhigher blastocyst formation was noted in CF-groupcompared to LF- (days 7, 8, 9) and SF- (days 8, 9) groups(experiment 1). In follicular fluid of small follicle group,β-GAL was associated positively with degeneratingoocytes’ number and negatively with blastocyst rate atdays 7, 8 (P = 0.065) and 9 (experiment 1). In fertilizationmedium of control group, α-MAN related negatively tocleavage rate (P < 0.05) and β-GAL to blastocyst rate atday 8 (P = 0.089) or day 9 (P = 0.072) (experiment 1).During fertilization, in experiment 1 all oocytesconsumed β-GAL and only control or small follicleoocytes consumed α-MAN; in experiment 1, only largefollicle oocytes released NAGASE, whereas all oocytesreleased all three glycosidases in experiment 2. Inconclusion, glycosidases affect the developmentalcompetence of oocytes collected from different sizedfollicles during in vitro fertilization, performed either ingroups or individually; their role in follicular fluid isdifferent from that in fertilization medium.

Association between three glycosidases activity A - mannosidase ( A-ΜΑΝ ), B-Ν-acetyloglucosaminidase (NAGASE) and B - galactosidase (B-GAL) andin vitro fertilization of bovine oocytes collected from different-sized follicles

We studied the role of three glycosidases (α-mannosidase - α-ΜΑΝ, β-N-acetyloglucosaminidase -NAGASE and β-galactosidase - β-GAL) in follicularfluid (FF) and in fertilization medium (FM) of bovineoocytes. Oocytes were allocated into 3 groups accordingto the follicular size (controls - CF: 2-8 mm, smallfollicle group - SF: 2-5 mm, large follicle group - LF:>5-8 mm). Bovine embryos were produced in vitroeither in groups (experiment 1, n = 2099 oocytes) orindividually (experiment 2, n = 79 oocytes). In bothexperiments, the activity of all glycosidases in the FF oflarge follicles was significantly lower than in the FF ofsmall follicles group. In the FM of LF-group oocytes, α-MAN and NAGASE were significantly higher comparedto SF- and CF-group oocytes (experiment 1) and β-GALwas significantly higher in SF- compared to CF-groupoocytes (experiment 2). Cleavage rate was similar amongall groups in both experiments; however significantlyhigher blastocyst formation was noted in CF-groupcompared to LF- (days 7, 8, 9) and SF- (days 8, 9) groups(experiment 1). In follicular fluid of small follicle group,β-GAL was associated positively with degeneratingoocytes number and negatively with blastocyst rate atdays 7, 8 (P = 0.065) and 9 (experiment 1). In fertilizationmedium of control group, α-MAN related negatively tocleavage rate (P < 0.05) and β-GAL to blastocyst rate atday 8 (P = 0.089) or day 9 (P = 0.072) (experiment 1).During fertilization, in experiment 1 all oocytesconsumed β-GAL and only control or small follicleoocytes consumed α-MAN; in experiment 1, only largefollicle oocytes released NAGASE, whereas all oocytesreleased all three glycosidases in experiment 2. Inconclusion, glycosidases affect the developmentalcompetence of oocytes collected from different sizedfollicles during in vitro fertilization, performed either ingroups or individually; their role in follicular fluid isdifferent from that in fertilization medium.(AU)

Transferência de embriões como ferramenta para formação de rebanho experimental ovino e análise dos fatores que podem maximizar a multiplicação do plantel / Embryo transfer as a tool to experimental sheep flock formation and stock multiplication factors analyses

Visando ao aumento do plantel de ovinos da raça Black Belly na estação experimental do Institut Scientifique de Recherche Agronomique (INRA) de Bourges, França, foram utilizadas 32 ovelhas da raça Black Belly superovuladas com FSH suíno nos últimos três dias de tratamento progestágeno de 14 dias. Após coleta cirúrgica, obtiveram-se 264 embriões transferíveis (8,2 ± 6,0/doadoras), sendo transferidos 232 a 88 receptoras da raça Romane pluríparas e 23 da raça Suffolk nulíparas. As taxas de parição e de sobrevivência embrionária foram 83,6 e 63%, respectivamente, sendo os melhores resultados observados em receptoras da raça Romane pluríparas do que nas nulíparas da raça Suffolk. O número de ovulações, de embriões transferidos e o estádio de desenvolvimento embrionário causaram efeito sobre a taxa de sobrevivência embrionária. As melhores taxas de parição e de sobrevivência embrionária foram obtidas de receptoras com mais de duas ovulações que receberam dois embriões no estádio de blastocisto.(AU)

With the purpose of increase the breeding of Black Belly sheep breed at the experimental station of the Institut Scientifique de Recherche Agronomique (INRA), Bourges, France, thirty-two Black Belly sheep were super ovulated with swine FSH on the last three days of a progesterone treatment. After surgical collect 264 embryos transferable were recovered (8.2 ± 6.0/ donor) and 232 were transferred to 88 multiparous Romane sheep receptors and 23 nulliparous Suffolk sheep. Birth rate and survival rate were 83.6 and 63% respectively, though the results form multiparous Romane sheep receptors were better than from nulliparous Suffolk sheep. The ovulations number, transferred embryos and development stage had an effect on embryo survival rate. The best birth and embryo survival rates were from receptors that had more than two ovulations and had two embryos implanted at the stage of blastocyst.(AU)

Transferência de embriões como ferramenta para formação de rebanho experimental ovino e análise dos fatores que podem maximizar a multiplicação do plantel / Embryo transfer as a tool to experimental sheep flock formation and stock multiplication factors analyses

Visando ao aumento do plantel de ovinos da raça Black Belly na estação experimental do Institut Scientifique de Recherche Agronomique (INRA) de Bourges, França, foram utilizadas 32 ovelhas da raça Black Belly superovuladas com FSH suíno nos últimos três dias de tratamento progestágeno de 14 dias. Após coleta cirúrgica, obtiveram-se 264 embriões transferíveis (8,2 ± 6,0/doadoras), sendo transferidos 232 a 88 receptoras da raça Romane pluríparas e 23 da raça Suffolk nulíparas. As taxas de parição e de sobrevivência embrionária foram 83,6 e 63%, respectivamente, sendo os melhores resultados observados em receptoras da raça Romane pluríparas do que nas nulíparas da raça Suffolk. O número de ovulações, de embriões transferidos e o estádio de desenvolvimento embrionário causaram efeito sobre a taxa de sobrevivência embrionária. As melhores taxas de parição e de sobrevivência embrionária foram obtidas de receptoras com mais de duas ovulações que receberam dois embriões no estádio de blastocisto.

With the purpose of increase the breeding of Black Belly sheep breed at the experimental station of the Institut Scientifique de Recherche Agronomique (INRA), Bourges, France, thirty-two Black Belly sheep were super ovulated with swine FSH on the last three days of a progesterone treatment. After surgical collect 264 embryos transferable were recovered (8.2 ± 6.0/ donor) and 232 were transferred to 88 multiparous Romane sheep receptors and 23 nulliparous Suffolk sheep. Birth rate and survival rate were 83.6 and 63% respectively, though the results form multiparous Romane sheep receptors were better than from nulliparous Suffolk sheep. The ovulations number, transferred embryos and development stage had an effect on embryo survival rate. The best birth and embryo survival rates were from receptors that had more than two ovulations and had two embryos implanted at the stage of blastocyst.