Condição metabólica e integridade das membranas, plasmática e acrossomal, da célula espermática bovina após criopreservação em diferentes meios diluidores acrescidos com diferentes tipos de insulina / Metabolic condition and integrity of membranes, and plasma acrosomal, cell bovine sperm after cryopreservation in different extenders greater with different kinds of insulin

Seminal plasma is a fluid with essential role in sperm functions in vivo, from ejaculation to fertilization.Among the hormones present in this medium, insulin stands out, due to its key role in the structure and motility,favoring fertilization. Insulin acts as preservation factor not able sperm and may be the crux of spermpreservation when using cryogenic processes. This study aimed to evaluate the metabolic and structuralcondition of bovine sperm cells after freezing using extenders plus different types of insulin and different types ofegg yolk. Our results showed that sperm motility was not affected by treatments used (insulin and yolk). Inconclusion, the type of egg yolk, the paw (Anas platyrhynchos), increased the viability of cryopreserved spermcells, regardless of the type of insulin used.(AU)

Condição metabólica e integridade das membranas, plasmática e acrossomal, da célula espermática bovina após criopreservação em diferentes meios diluidores acrescidos com diferentes tipos de insulina / Metabolic condition and integrity of membranes, and plasma acrosomal, cell bovine sperm after cryopreservation in different extenders greater with different kinds of insulin

Seminal plasma is a fluid with essential role in sperm functions in vivo, from ejaculation to fertilization.Among the hormones present in this medium, insulin stands out, due to its key role in the structure and motility,favoring fertilization. Insulin acts as preservation factor not able sperm and may be the crux of spermpreservation when using cryogenic processes. This study aimed to evaluate the metabolic and structuralcondition of bovine sperm cells after freezing using extenders plus different types of insulin and different types ofegg yolk. Our results showed that sperm motility was not affected by treatments used (insulin and yolk). Inconclusion, the type of egg yolk, the paw (Anas platyrhynchos), increased the viability of cryopreserved spermcells, regardless of the type of insulin used.

Qualidade e Quantidade de Oócitos Caprinos e Ovinos Recuperados por Aspiração e Fatiamento / Quality and Quantity of Goats and Sheep Oocytes Recovered by Bspiration and Slicing

This study aimed to compare the aspiration techniques and slicing ovaries in order to obtain oocytes, byassessing the quantity and quality of oocytes recovered from goat and sheep females slaughtered in São Luís -MA. The ovaries were collected after slaughtering and kept in saline solution at 0.9%, containing 30 µg / mL ofgentamicin sulfate. In the laboratory the ovaries were randomly divided into two groups with subsequent processof aspiration and slicing. The average rate of oocyte retrieval by aspiration technique were 4,53 oocytes/ovaryin sheeps and 4,11 oocytes/ovary in goats, and by the slicing technique 5,26 oocytes/ovary in sheeps and 5,06oocytes/ovary in goats. It was not observed a significant difference between the recovery rate between species,however there was statistical difference between the techniques on goats, therefore demonstrating that the twotechniques can be used for oocyte retrieval.(AU)

Qualidade e Quantidade de Oócitos Caprinos e Ovinos Recuperados por Aspiração e Fatiamento / Quality and Quantity of Goats and Sheep Oocytes Recovered by Bspiration and Slicing

This study aimed to compare the aspiration techniques and slicing ovaries in order to obtain oocytes, byassessing the quantity and quality of oocytes recovered from goat and sheep females slaughtered in São Luís -MA. The ovaries were collected after slaughtering and kept in saline solution at 0.9%, containing 30 µg / mL ofgentamicin sulfate. In the laboratory the ovaries were randomly divided into two groups with subsequent processof aspiration and slicing. The average rate of oocyte retrieval by aspiration technique were 4,53 oocytes/ovaryin sheeps and 4,11 oocytes/ovary in goats, and by the slicing technique 5,26 oocytes/ovary in sheeps and 5,06oocytes/ovary in goats. It was not observed a significant difference between the recovery rate between species,however there was statistical difference between the techniques on goats, therefore demonstrating that the twotechniques can be used for oocyte retrieval.