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1.
Acta sci., Anim. sci ; 45: e57700, 2023. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1396714

Resumo

The present study aimed to correlate the influence of water quality, cultivation time, area, and density of ponds on the zootechnical performance of shrimp Litopenaeus vannameiin oligohaline waters. It was demonstrated that, under the conditions of this research, better control of temperature, pH and water alkalinity can be employed. Ammonia levels were within the normal range and correlated positively with the growth rate (ρ= 0.69859) and production (ρ= 0.66362) of the ponds. There was also a positive correlation between cultivation time and pond density with shrimp weight (ρ= 0.75305 and 0.68933) and pond productivity (ρ= 0.74763 and 0.79367, respectively). Negative correlations were observed between: I) pH with shrimp survival (ρ= -0.73238) and pondproduction (ρ= -0.68696); II) alkalinity and pond production (ρ= -0.66162). The results show that the cultivation of shrimp L.vannameiin oligohaline waters is viable and there is potential for increased productivity associated with better hydrologicalmonitoring and use of higher density and cultivation time by some producers.(AU)


Assuntos
Animais , Qualidade da Água , Penaeidae/crescimento & desenvolvimento , Recursos Hídricos , Aquicultura
2.
Anim. Reprod. (Online) ; 16(2): 260-266, abr.-jun. 2019. tab
Artigo em Inglês | VETINDEX | ID: biblio-1461432

Resumo

Transvaginal follicular aspiration technique together with in vitro embryo production are the biotechnological alternatives currently available to support genetic improvement breeding programs in buffalo species. However, aspects related to animal management, lack of knowledge of the metabolic needs and biochemical peculiarities of gametes and embryos, as well as the reproductive physiology characteristics have hampered progress in the results. Despite the low availability of good quality oocytes collected after OPU in donors as a physiological characteristic of buffalo species, high rates of oocyte maturation, modest embryo cleavage, blastocyst production and pregnancy rates after transvaginal embryo transfer in recipients could be obtained in buffalo in vitro embryo production programs. The results of implementing an in vitro embryo production program in buffaloes in the northern region of Pará state, Brazil, and results published by other groups demonstrate the feasibility of implementing this biotechnology in the routine of breeding programs. Nevertheless, in order to achieve better and consistent results, it is necessary to deepen the knowledge on the peculiarities of reproductive biology in this specie. Selection of donor animals based on ovarian size and ovarian follicular reserve and on the rate of blastocyst production is presented as an effective alternative to increase the efficiency of the in vitro embryo production technique applied to the buffalo species.


Assuntos
Animais , Bovinos , Búfalos/embriologia , Desenvolvimento Embrionário , Melhoramento Genético
3.
Anim. Reprod. ; 16(2): 260-266, abr.-jun. 2019. tab
Artigo em Inglês | VETINDEX | ID: vti-20543

Resumo

Transvaginal follicular aspiration technique together with in vitro embryo production are the biotechnological alternatives currently available to support genetic improvement breeding programs in buffalo species. However, aspects related to animal management, lack of knowledge of the metabolic needs and biochemical peculiarities of gametes and embryos, as well as the reproductive physiology characteristics have hampered progress in the results. Despite the low availability of good quality oocytes collected after OPU in donors as a physiological characteristic of buffalo species, high rates of oocyte maturation, modest embryo cleavage, blastocyst production and pregnancy rates after transvaginal embryo transfer in recipients could be obtained in buffalo in vitro embryo production programs. The results of implementing an in vitro embryo production program in buffaloes in the northern region of Pará state, Brazil, and results published by other groups demonstrate the feasibility of implementing this biotechnology in the routine of breeding programs. Nevertheless, in order to achieve better and consistent results, it is necessary to deepen the knowledge on the peculiarities of reproductive biology in this specie. Selection of donor animals based on ovarian size and ovarian follicular reserve and on the rate of blastocyst production is presented as an effective alternative to increase the efficiency of the in vitro embryo production technique applied to the buffalo species.(AU)


Assuntos
Animais , Bovinos , Desenvolvimento Embrionário , Búfalos/embriologia , Melhoramento Genético
4.
Ci. Rural ; 48(6): e20170771, July 16, 2018. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-738907

Resumo

This study aimed to identify the principal components (PC) that explain the highest percentages of total variance and best characterize the in vivo and carcass morphologies of Anglo-Nubian crossbred goats. Nineteen carcass morphometric traits and six in vivo morphometric traits were measured in 28 kids at eight months of age. Principal component analysis indicated that five PC were able to explain 83.57% of the total variance in the 19 original carcass traits. Those components were termed PC1-Carcass Size, PC2 - Body Condition, PC3-Carcass Width, PC4-Chest Depth, and PC5 - Hindquarter. For in vivo morphometric traits, the first two principal components explained 78.86% of the total variance. These components were called PC1-In vivo Size and PC2-In vivo Conformation.(AU)


Este estudo buscou identificar componentes principais (CP) que explicam os maiores percentuais de variância total e que melhor caracterizam cabritos mestiços da raça Anglo Nubiana, quanto à medidas morfológicas obtidas in vivo, e na carcaça de 28 animais com 8 meses de idade. Foram conduzidas duas análises de componentes principais, sendo uma para 19 características de carcaça e outra para seis características morfométricas in vivo. Os cinco primeiros CP explicaram 82,54% da variância total das 19 características incluídas nessa análise. Estes componentes foram chamados de: CP1 - Tamanho da Carcaça, CP2 - Condição Corporal, CP3 - Largura da Carcaça, CP4 - Profundidade do Tórax e, CP5 - Comprimento do Pernil. Os dois primeiros componentes principais das morfometrias obtidas in vivo explicaram 78,86% da variância total e foram chamados de CP1 - Tamanho in vivo e CP2 - Conformação in vivo.(AU)


Assuntos
Animais , Ruminantes , Análise Multivariada , Tamanho Corporal , Criação de Animais Domésticos
5.
R. bras. Reprod. Anim. ; 40(4): 270-271, Out-Dez. 2016. tab
Artigo em Português | VETINDEX | ID: vti-24001

Resumo

The objective of this study was to evaluate if the progesterone device (P4) of 3rd use would be efficientto induce the cyclicity and consequent pregnancy rate in nelore heifers. We used 65 animals with a mean weightof 338 kg. All kept in the same environmental and handling conditions. The induction of estrus was performed in33 females with P4 implant of 3rd use, thus forming Group 1 (G1 = 33), those that did not receive the implantwere allocated in Group 2 (G2 = 32). Thirty-two days post-induction protocol was performed on all animals forTAI. The data collected were tabulated in electronic spreadsheets and evaluated in the SAS software (2010). TheTAI pregnancy rate was 21.21% (G1) (7/33) and 46.88% (15/32) for (G2) (P < 0.05). Thus suggesting that priorinduction to puberty with P4 device of 3rd use was not effective.(AU)


Assuntos
Animais , Feminino , Bovinos , Prenhez , Maturidade Sexual , Progesterona/análise , Bovinos/embriologia
6.
Rev. bras. reprod. anim ; 40(4): 270-271, Out-Dez. 2016. tab
Artigo em Português | VETINDEX | ID: biblio-1492261

Resumo

The objective of this study was to evaluate if the progesterone device (P4) of 3rd use would be efficientto induce the cyclicity and consequent pregnancy rate in nelore heifers. We used 65 animals with a mean weightof 338 kg. All kept in the same environmental and handling conditions. The induction of estrus was performed in33 females with P4 implant of 3rd use, thus forming Group 1 (G1 = 33), those that did not receive the implantwere allocated in Group 2 (G2 = 32). Thirty-two days post-induction protocol was performed on all animals forTAI. The data collected were tabulated in electronic spreadsheets and evaluated in the SAS software (2010). TheTAI pregnancy rate was 21.21% (G1) (7/33) and 46.88% (15/32) for (G2) (P < 0.05). Thus suggesting that priorinduction to puberty with P4 device of 3rd use was not effective.


Assuntos
Feminino , Animais , Bovinos , Bovinos/embriologia , Maturidade Sexual , Prenhez , Progesterona/análise
7.
Braz. J. Microbiol. ; 47(2): 403-409, Abr-Jun. 2016. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-23450

Resumo

Considering the absence of standards for culture collections and more specifically for biological resource centers in the world, in addition to the absence of certified biological material in Brazil, this study aimed to evaluate a Fungal Collection from Fiocruz, as a producer of certified reference material and as Biological Resource Center (BRC). For this evaluation, a checklist based on the requirements of ABNT ISO GUIA34:2012 correlated with the ABNT NBR ISO/IEC17025:2005, was designed and applied. Complementing the implementation of the checklist, an internal audit was performed. An evaluation of this Collection as a BRC was also conducted following the requirements of the NIT-DICLA-061, the Brazilian internal standard from Inmetro, based on ABNT NBR ISO/IEC 17025:2005, ABNT ISO GUIA 34:2012 and OECD Best Practice Guidelines for BRCs. This was the first time that the NIT DICLA-061 was applied in a culture collection during an internal audit. The assessments enabled the proposal for the adequacy of this Collection to assure the implementation of the management system for their future accreditation by Inmetro as a certified reference material producer as well as its future accreditation as a Biological Resource Center according to the NIT-DICLA-061.(AU)


Assuntos
Materiais Biocompatíveis , Micobioma , Serviços de Informação/instrumentação , Serviços de Informação/organização & administração
8.
Braz. J. Microbiol. ; 46(1): 49-57, Jan.- Mar. 2015. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-481360

Resumo

Bauhinia forficata is native to South America and used with relative success in the folk medicine in Brazil. The diversity, antibacterial activity, and extracellular hydrolytic enzymes of endophytic fungi associated with this plant were studied. Plant samples, which included leaves, sepals, stems, and seeds, were used. Ninety-five endophytic fungal were isolated (18 from leaves, 22 from sepals, 46 from stems, and nine from seeds), comprising 28 species. The most frequently isolated species were Acremonium curvulum (9.5%), Aspergillus ochraceus (7.37%), Gibberella fujikuroi (10.53%), Myrothecium verrucaria (10.53%) and Trichoderma piluliferum (7.37%). Diversity and species richness were higher in stem tissues, and Sorensens index of similarity between the tissues was low. Eleven fungi showed antibacterial activity. Aspergillus ochraceus, Gibberella baccata, Penicillium commune, and P. glabrum were those with the greatest antibacterial activity against Staphylococcus aureus and/or Streptococcus pyogenes. Thirteen species showed proteolytic activity, particularly Phoma putaminum. Fourteen species were cellulase positive, particularly the Penicillium species and Myrmecridium schulzeri. All isolates tested were xylanase positive and 10 showed lipolytic activity, especially Penicillium glabrum. It is clear that the endophytic fungi from B. forficata have potential for the production of bioactive compounds and may be a source of new therapeutic agents for the effective treatment of diseases in humans, other animals, and plants. To our knowledge, this is the first study of endophytic fungi from different tissues of B. forficata and their biotechnological potential.(AU)


Assuntos
Anti-Infecciosos/metabolismo , Bauhinia/microbiologia , Biodiversidade , Endófitos/classificação , Fungos/metabolismo , Bactérias , Produtos Biológicos/metabolismo , Biotecnologia/métodos , Brasil , Endófitos/isolamento & purificação
9.
Bol. Inst. Pesca (Impr.) ; 38(4): 297-312, 2012.
Artigo em Português | LILACS-Express | VETINDEX | ID: biblio-1464960

Resumo

This study aimed to carry out malacological samples of limnic mollusks present in a commercial fishing pond area on Itapecerica da Serra, São Paulo/Brazil, between July 2006 to June 2007. Furthermore, parasitological studies were also performed on specimens found. A total of 19,863 limnic mollusks were collected, represented by six species: Biomphalaria straminea Dunker, 1848; Melanoides tuberculatus Müller, 1774; Lymnaea columella Say, 1817; Pomacea lineata Spix, 1827; Physa marmorata Guilding, 1828 and Anodontites trapesialis Lamarck, 1819, being this the first report of the occurrence of this species in fish and pay systems of Itapecerica da Serra. The parasitological examinations performed in 5,766 mollusks were negative, however, the greatest abundance of B. straminea, intermediate host of Schistosoma mansoni Sambon, 1907, becomes a concern. Thus, the need for increasing the malacological surveillance in the region due to the diversity of hydric collections in the municipality that has precarious sanitation conditions, high migration flow and the presence of imported and autochthonous cases of schistosomiasis.


Este trabalho teve como objetivo realizar levantamento da malacofauna límnica encontrada na área de um pesqueiro em Itapecerica da Serra, São Paulo - Brasil, no período de julho de 2006 a junho de 2007. Como complementação, foram feitos estudos parasitológicos em parte dos moluscos amostrados. Foram coletados 19.863 moluscos límnicos, representados por seis espécies: Biomphalaria straminea Dunker, 1848; Melanoides tuberculatus Müller, 1774; Lymnaea columella Say, 1817; Pomacea lineata Spix, 1827; Physa marmorata Guilding, 1828 e Anodontites trapesialis Lamarck, 1819. Este pode ser considerado o primeiro relato da ocorrência dessas espécies em pesqueiros de Itapecerica da Serra. Os exames parasitológicos realizados em 5.766 moluscos foram negativos, no entanto, a presença de B. straminea, hospedeiro intermediário do Schistosoma mansoni Sambon, 1907 é preocupante. Diante disso, é necessário intensificar a vigilância malacológica na região devido à diversidade de coleções hídricas do município, precárias condições de saneamento básico, alto fluxo migratório de pessoas e o relato de casos importados e autóctones de esquistossomose.

10.
Ci. Rural ; 38(4): 960-966, jul.-ago. 2008. tab
Artigo em Português | VETINDEX | ID: vti-4524

Resumo

A pata-de-vaca (Bauhinia divaricata) é uma espécie arbórea, amplamente distribuída no Brasil, de alto valor ornamental e econômico. Sua propagação ocorre por meio de sementes, cuja germinação tem sido pouco investigada. Dessa forma, o presente trabalho teve como objetivo definir o tipo de substrato e a temperatura mais adequados para avaliar a germinação e o vigor de sementes de Bauhinia divaricata. O experimento foi realizado no Laboratório de Análise de Sementes do CCA-UFPB, em Areia-PB, em delineamento inteiramente casualizado com os tratamentos distribuídos em esquema fatorial 3 x 5, com os fatores temperaturas constantes de 25 e 30ºC e alternada 20-30ºC e substratos entre papel, sobre papel, rolo de papel, entre areia e entre vermiculita, em quatro repetições de 25 sementes, em câmaras tipo BOD, com fotoperíodo de oito horas. Foram analisadas as seguintes variáveis: porcentagem de germinação, primeira contagem e índice de velocidade de germinação e massa seca de plântulas. Concluiu-se que a temperatura de 25ºC, juntamente com os substratos entre papel, sobre papel e rolo de papel, é adequada para condução de testes de germinação e vigor com sementes de Bauhinia divaricata. O substrato areia nas três temperaturas (20-30, 25 e 30ºC) foi responsável pelas menores porcentagens de germinação e níveis de vigor das sementes.(AU)


The Bauhinia divaricata is an arboreal species with high ornamental and economical value and distributed throughout Brazil. It is propagated by seeds, from which germination needs more investigation. So, this study was carried out at the Seed Analysis Laboratory pertaining to CCA-UFPB - Areia, in BOD-type chambers with 8h photoperiod in order to determine the most adequate substratum and temperature for evaluating the germination and vigor of the Bauhinia divaricata seeds. The entirely randomized experimental design in the factorial scheme 3 x 5 was used. The factors were constituted by constant temperatures of 25 and 30ºC and alternate 20-30ºC, as well as substrata between paper, on paper, paper roll, among sand and among vermiculite, in four replicates of 25 seeds. The following variables were analyzed: percent germination, first counting, germination index and dry matter of the plantlets. The 25ºC temperature and the substrata between paper, on paper and paper roll showed to be adequate to the test for germination and vigor of the Bauhinia divaricata seeds. The lowest germination percentages and the seed vigor levels occurred in the sand substratum at three temperatures (20-30, 25 and 30ºC).(AU)


Assuntos
Bauhinia , Germinação
11.
Artigo em Inglês | VETINDEX | ID: vti-443651

Resumo

Primary cell culture from rabbit meniscus (fibrochondrocytes-FcrC) was infected for 24 hours with different inocula (10² to 10(7) Colony Forming Units-CFU) of Mycoplasma hominis PG-21, M. pneumoniae FH and 1428 or M. arthritidis PG-6. The severity of the different obtained cytophatic effects-CPE was inoculum, Mycoplasma species and strain dependant. These bacteria were recovered from all infected FcrC and the SP4 medium for mycoplasmas also caused toxic effect on the FcrC. It was concluded that rabbit fibrochondrocytes were sensitive to mycoplasma infection, as well as to the SP4 mycoplasma medium.


Cultura primária de menisco de coelho (Fibrocondrócito-FcrC) foi infectada por 24 horas com diferentes inóculos (10² to 10(7) Unidade Formadoras de Colonias-UFC) de Mycoplasma hominis PG-21, M. pneumoniae FH e 1428 ou M. arthritidis PG-6. A severidade dos diferentes efeitos citopáticos-EC foram dependentes do inóculo, espécie e cepa de micoplasma. Estas bactérias foram reisoladas de todos os FcrC infectados e o meio SP4 para micoplasmas também causou efeito tóxico para os FcrC. Concluiu-se que os fibrocondrócitos de coelho foram sensíveis à infecção por micoplasmas e também ao meio SP4.

12.
B. Inst. Pesca ; 38(4): 297-312, 2012.
Artigo em Português | VETINDEX | ID: vti-760851

Resumo

This study aimed to carry out malacological samples of limnic mollusks present in a commercial fishing pond area on Itapecerica da Serra, São Paulo/Brazil, between July 2006 to June 2007. Furthermore, parasitological studies were also performed on specimens found. A total of 19,863 limnic mollusks were collected, represented by six species: Biomphalaria straminea Dunker, 1848; Melanoides tuberculatus Müller, 1774; Lymnaea columella Say, 1817; Pomacea lineata Spix, 1827; Physa marmorata Guilding, 1828 and Anodontites trapesialis Lamarck, 1819, being this the first report of the occurrence of this species in fish and pay systems of Itapecerica da Serra. The parasitological examinations performed in 5,766 mollusks were negative, however, the greatest abundance of B. straminea, intermediate host of Schistosoma mansoni Sambon, 1907, becomes a concern. Thus, the need for increasing the malacological surveillance in the region due to the diversity of hydric collections in the municipality that has precarious sanitation conditions, high migration flow and the presence of imported and autochthonous cases of schistosomiasis.


Este trabalho teve como objetivo realizar levantamento da malacofauna límnica encontrada na área de um pesqueiro em Itapecerica da Serra, São Paulo - Brasil, no período de julho de 2006 a junho de 2007. Como complementação, foram feitos estudos parasitológicos em parte dos moluscos amostrados. Foram coletados 19.863 moluscos límnicos, representados por seis espécies: Biomphalaria straminea Dunker, 1848; Melanoides tuberculatus Müller, 1774; Lymnaea columella Say, 1817; Pomacea lineata Spix, 1827; Physa marmorata Guilding, 1828 e Anodontites trapesialis Lamarck, 1819. Este pode ser considerado o primeiro relato da ocorrência dessas espécies em pesqueiros de Itapecerica da Serra. Os exames parasitológicos realizados em 5.766 moluscos foram negativos, no entanto, a presença de B. straminea, hospedeiro intermediário do Schistosoma mansoni Sambon, 1907 é preocupante. Diante disso, é necessário intensificar a vigilância malacológica na região devido à diversidade de coleções hídricas do município, precárias condições de saneamento básico, alto fluxo migratório de pessoas e o relato de casos importados e autóctones de esquistossomose.

13.
Semina ciênc. agrar ; 33(6): 3023-3032, 2012.
Artigo em Português | LILACS-Express | VETINDEX | ID: biblio-1498972

Resumo

As cinzas provenientes da queima de carvão mineral, seja in natura ou zeolitizada, possuem estrutura e características químicas semelhantes às zeólitas. Verificou-se a adição destes materiais à uréia nas proporções ureia:cinzas 100:10, 100:20, 100:50, 100:100 e um controle (somente ureia), em delineamento com blocos casualizados em solo arenoso e argiloso sem incorporação, avaliando as perdas de nitrogênio por volatilização de amônia e os parâmetros químicos de fertilidade do solo. As proporções 100:10 e 100:20 promoveram redução significativa de perdas por volatilização da amônia, porém somente no solo de textura arenosa. Não há diferença entre a cinza zeolitilizada e a cinza in natura visando à redução das perdas de N por volatilização de NH3 da ureia.


The structural and chemical characteristics of fly ash from coal-fired mineral and fly ash zeolitized are similar to those of zeolites. Urea was added with these materials in the proportions of urea: fly ashes of 100:10, 100:20, 100:50, 100:100, with a control containing just urea. These treatments were applied in soil surface and the experimental design was a randomized block with clay and sandy soil. Nitrogen losses by ammonia volatilization and the chemical characteristics of soil fertility were evaluated. In sandy soil there was reduction of ammonia volatilization for the proportions of 100:10 and 100:20, while fly ash zeolitized and fly ash had no difference.

14.
Semina Ci. agr. ; 33(6): 3023-3032, 2012.
Artigo em Português | VETINDEX | ID: vti-470314

Resumo

As cinzas provenientes da queima de carvão mineral, seja in natura ou zeolitizada, possuem estrutura e características químicas semelhantes às zeólitas. Verificou-se a adição destes materiais à uréia nas proporções ureia:cinzas 100:10, 100:20, 100:50, 100:100 e um controle (somente ureia), em delineamento com blocos casualizados em solo arenoso e argiloso sem incorporação, avaliando as perdas de nitrogênio por volatilização de amônia e os parâmetros químicos de fertilidade do solo. As proporções 100:10 e 100:20 promoveram redução significativa de perdas por volatilização da amônia, porém somente no solo de textura arenosa. Não há diferença entre a cinza zeolitilizada e a cinza in natura visando à redução das perdas de N por volatilização de NH3 da ureia.


The structural and chemical characteristics of fly ash from coal-fired mineral and fly ash zeolitized are similar to those of zeolites. Urea was added with these materials in the proportions of urea: fly ashes of 100:10, 100:20, 100:50, 100:100, with a control containing just urea. These treatments were applied in soil surface and the experimental design was a randomized block with clay and sandy soil. Nitrogen losses by ammonia volatilization and the chemical characteristics of soil fertility were evaluated. In sandy soil there was reduction of ammonia volatilization for the proportions of 100:10 and 100:20, while fly ash zeolitized and fly ash had no difference.

15.
Tese em Português | VETTESES | ID: vtt-7558

Resumo

O polimorfismo do gene BoLA-DRB3.2 foi estudado em 1.058 vacas pertencentes a sete rebanhos Gir Leiteiro. Os resultados permitem concluir que há grande polimorfismo na sequência deste gene, na raça Gir, tendo sido identificados 15 alelos (BoLA-DRB3*9, *12, *15, *16, *22, *23, *24, *26, *36, *38, *45, *46,*51, *52 e *54), não citados anteriormente em raças Zebuínas, de um total de 37 alelos encontrados. As frequências alélicas diferiram entre os sete rebanhos analisados. Foram encontrados 11 alelos comuns, sendo o alelo BoLA-DRB3*20 o mais freqüente (19,04%) na população total. Pela análise do efeito de substituição gênica foi identificada uma associação altamente significativa entre o alelo BoLA-DRB3*54 (P<0,02) e diminuição (-26,1kg) da produção de proteína do leite. Outros alelos DRB3*6 e DRB3*7 mostraram associações significativas (P<0,05) para diminuição (-12,47 kg) e aumento (12,72 kg) de proteína do leite, respectivamente. O alelo DRB3*54 também foi associado significativamente (P<0,05) com diminuição (-26,07 kg) na produção de gordura do leite. Para o escore de células somáticas (ECS), foram encontradas associações (P<0,10) com os alelos DRB3*3 e DRB3*31. A associação altamente significativa do alelo BoLA-DRB*54 com menor produção de proteína sugere a possibilidade de utilização desse alelo em programas de Seleção Assistida com Marcadores (MAS)

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