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1.
Ciênc. Anim. (Impr.) ; 29(1,supl.1): 41-44, 2019. tab
Artigo em Português | VETINDEX | ID: biblio-1472476

Resumo

O acompanhamento gestacional por ultrassonografia permite avaliar o desenvolvimento do concepto e estimar a idade gestacional (IG). No entanto, a fórmula de Nyland e Mattoon para cálculo da IG foi desenvolvida no Beagle. Cães braquicefálicos como o Buldogue Francês apresentam maior predisposição à distocia, o que torna ainda mais importante a correta estimativa da IG. Objetivou-se avaliar a fórmula de Nyland e Mattoon para datar a IG em Buldogue Francês. Cinco cadelas foram submetidas à ultrassonografia abdominal aos 55 dias pós-inseminação. Foi mensurado o diâmetro biparietal (DBP) fetal e aplicado na fórmula. Os resultados foram expressos na forma de média e erro padrão. O DBP foi de 2,14±0,02 e a IG correspondeu a 52 dias. A fórmula não tem confiabilidade para estimar a IG nesta raça.


The ultrasound gestational follow-up allows the evaluation of the development of the concept and the estimation of gestational age (GI). However, the Nyland and Mattoon formula for calculating the GI was developed in the Beagle. Brachycephalic dogs such as the French Bulldog have a greater predisposition to dystocia, which makes the correct estimation of GI even more important. The objective of this study was to evaluate the Nyland and Mattoon formula for dating GI in French Bulldog. Five bitches were submitted to abdominal ultrasound at 55 days post-insemination. The fetal biparietal diameter (DBP) was measured and applied in the formula. The results were expressed as mean and standard error. DBP was 2.14±0.02 and IG corresponded to 52 days. The formula has no reliability to estimate GI in this breed.


Assuntos
Feminino , Animais , Cães , Distocia/prevenção & controle , Distocia/veterinária , Idade Gestacional , Prenhez , Ultrassonografia/métodos , Ultrassonografia/veterinária , Osso Parietal
2.
Ciênc. Anim. (Impr.) ; 29(1,supl.1): 41-44, 2019. tab
Artigo em Português | VETINDEX | ID: vti-21583

Resumo

O acompanhamento gestacional por ultrassonografia permite avaliar o desenvolvimento do concepto e estimar a idade gestacional (IG). No entanto, a fórmula de Nyland e Mattoon para cálculo da IG foi desenvolvida no Beagle. Cães braquicefálicos como o Buldogue Francês apresentam maior predisposição à distocia, o que torna ainda mais importante a correta estimativa da IG. Objetivou-se avaliar a fórmula de Nyland e Mattoon para datar a IG em Buldogue Francês. Cinco cadelas foram submetidas à ultrassonografia abdominal aos 55 dias pós-inseminação. Foi mensurado o diâmetro biparietal (DBP) fetal e aplicado na fórmula. Os resultados foram expressos na forma de média e erro padrão. O DBP foi de 2,14±0,02 e a IG correspondeu a 52 dias. A fórmula não tem confiabilidade para estimar a IG nesta raça.(AU)


The ultrasound gestational follow-up allows the evaluation of the development of the concept and the estimation of gestational age (GI). However, the Nyland and Mattoon formula for calculating the GI was developed in the Beagle. Brachycephalic dogs such as the French Bulldog have a greater predisposition to dystocia, which makes the correct estimation of GI even more important. The objective of this study was to evaluate the Nyland and Mattoon formula for dating GI in French Bulldog. Five bitches were submitted to abdominal ultrasound at 55 days post-insemination. The fetal biparietal diameter (DBP) was measured and applied in the formula. The results were expressed as mean and standard error. DBP was 2.14±0.02 and IG corresponded to 52 days. The formula has no reliability to estimate GI in this breed.(AU)


Assuntos
Animais , Feminino , Cães , Prenhez , Idade Gestacional , Ultrassonografia/veterinária , Ultrassonografia/métodos , Distocia/veterinária , Distocia/prevenção & controle , Osso Parietal
3.
Anim. Reprod. (Online) ; 14(4): 1095-1102, Oct.-Dec. 2017. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1461305

Resumo

The aims of this study were to investigate the effects of different concentrations of Platelet-derived growth factor-BB (PDGF-BB) on the survival, activation, levels of ROS, and growth of goat preantral follicles enclosed in ovarian tissue. For this, ovarian fragments were cultured for 7 days in Alpha Minimum Essential Medium (α-MEM+ ) with or without PDGF-BB (0, 25, 50 and 100 ng/ml). The results showed that both the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments maintained the percentage of morphologically normal follicles from day 1 to day 7. In addition, the 25 ng/ml PDGF treatment showed a significantly higher percentage of morphologically normal follicles when compared to the other treatments. At day 7, greater (P < 0.05) follicular and oocyte diameters were observed in the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments when compared to the cultured control treatment. On day 7 of culture, all the treatments tested had a significant increase in the percentage of developing follicles when compared to the non-cultured control. However, the percentage of follicle activation, as well as ROS production, were similar (P < 0.05) among the treatments, irrespective of culture time. In conclusion, PDGF-BB improved, in a concentration-dependent manner, follicular survival as well as oocyte and follicular diameter after in vitro culture of goat preantral follicle-enclosed in ovarian tissue fragments.


Assuntos
Animais , Cabras/anatomia & histologia , Cabras/embriologia , Fator de Crescimento Derivado de Plaquetas/administração & dosagem , Fator de Crescimento Derivado de Plaquetas/efeitos adversos , Fertilização in vitro , Folículo Ovariano
4.
Anim. Reprod. ; 14(4): 1095-1102, Oct.-Dec. 2017. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: vti-18213

Resumo

The aims of this study were to investigate the effects of different concentrations of Platelet-derived growth factor-BB (PDGF-BB) on the survival, activation, levels of ROS, and growth of goat preantral follicles enclosed in ovarian tissue. For this, ovarian fragments were cultured for 7 days in Alpha Minimum Essential Medium (α-MEM+ ) with or without PDGF-BB (0, 25, 50 and 100 ng/ml). The results showed that both the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments maintained the percentage of morphologically normal follicles from day 1 to day 7. In addition, the 25 ng/ml PDGF treatment showed a significantly higher percentage of morphologically normal follicles when compared to the other treatments. At day 7, greater (P < 0.05) follicular and oocyte diameters were observed in the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments when compared to the cultured control treatment. On day 7 of culture, all the treatments tested had a significant increase in the percentage of developing follicles when compared to the non-cultured control. However, the percentage of follicle activation, as well as ROS production, were similar (P < 0.05) among the treatments, irrespective of culture time. In conclusion, PDGF-BB improved, in a concentration-dependent manner, follicular survival as well as oocyte and follicular diameter after in vitro culture of goat preantral follicle-enclosed in ovarian tissue fragments.(AU)


Assuntos
Animais , Fator de Crescimento Derivado de Plaquetas/administração & dosagem , Fator de Crescimento Derivado de Plaquetas/efeitos adversos , Cabras/anatomia & histologia , Cabras/embriologia , Fertilização in vitro , Folículo Ovariano
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