First report of fibropapillomatosis (FP) and Chelonid alphaherpesvirus 5 (ChHV5) in a green sea turtle (Chelonia mydas) from the historically fibropapillomatosis-free Fernando de Noronha Archipelago, Northeastern Brazil / Primeiro caso de fibropapilomatose (FP) e de Chelonid alphaherpesvirus 5 (ChHV5) em tartaruga-verde (Chelonia mydas) no Arquipélago de Fernando de Noronha, Nordeste do Brasil

Fibropapillomatosis (FP) is an infectious disease caused by Chelonid alphaherpesvirus 5 (ChHV5). Nevertheless, its clinical manifestations are considered multifactorial. Due to its relevance, FP is currently monitored in sea turtle populations in the United States, Australia, Caribbean, and Brazil. Between 2000 and 2020, the TAMAR Project/ TAMAR Project Foundation analyzed the prevalence of FP in nine states and oceanic islands along the Brazilian coast, including Fernando de Noronha Archipelago (FNA), a historically FP-free area. A total of 4,435 green sea turtles (Chelonia mydas) were monitored from 2010 to 2016. Additionally, in 2012 and 2014, 43 FP-free skin samples were analyzed for ChHV5 using a qualitative PCR for the UL30 polymerase (pol) sequence. In 2015, a bilateral ocular nodule characterized as an FP tumor was reported in one of the monitored individuals undergoing rehabilitation. Tissue samples were collected following surgical removal of the tumor. Characterization of a 454 bp UL30 polymerase gene revealed a ChHV5 sequence previously reported in other areas of the Atlantic Brazilian coast. In the years following this finding from January 2017 to March 2020, a total of 360 C. mydas were monitored in the same area and no FP tumors were detected. This is the first report of FP and the first detection of ChHV5 in FNA, a finding of great concern considering this site's historical absence of FP occurrence. This study highlights the importance of monitoring this disease in historically FP-free areas of the Brazilian Atlantic coast.(AU)

A fibropapilomatose (FP) é uma doença infecciosa causada pelo Chelonid alphaherpesvirus 5 (ChHV5). No entanto, as manifestações clínicas da doença são consideradas multifatoriais. Esta doença é monitorada atualmente em populações de tartarugas marinhas nos EUA, Austrália, Caribe e Brasil. Desde 2000, o Projeto TAMAR/Fundação Projeto TAMAR analisa a presença de FP em nove estados da costa brasileira e ilhas oceânicas, incluindo o arquipélago de Fernando de Noronha, uma área historicamente livre de FP. Um total de 4.435 indivíduos de Chelonia mydas foram monitorados de 2010 a 2016 e 43 amostras de pele foram analisadas para detectar ChHV5 em 2012 e 2014 com o objetivo de avaliar a presença do vírus em tecidos sem FP, usando uma PCR qualitativa para detecção de sequências do gene da UL30 polimerase. Em 2015, uma tartaruga verde (C. mydas) foi relatada com um nódulo ocular bilateral caracterizado como FP. Amostras de tecido foram coletadas durante sua reabilitação e procedimento cirúrgico para remover o tumor. A caracterização parcial de uma sequência de 454 bp do gene UL30 polimerase detectou ChHV5 anteriormente relatado em outras áreas da costa atlântica brasileira. Após estes achados, de janeiro de 2017 a março de 2020, um total de 360 indivíduos de C. mydas foram monitorados e nenhum caso de FP foi registrado. Este é o primeiro relato de FP e a primeira caracterização de ChHV5 no arquipélago de Fernando de Noronha, uma questão preocupante e que ressalta a importância do monitoramento desta doença em áreas historicamente livres de FP na costa atlântica brasileira.(AU)

Prolapso peniano em tartaruga-verde (Chelonia mydas) / Penile prolapse in green sea turtle (Chelonia mydas)

A ocorrência de prolapso peniano em repteis é observada tanto em quelônios terrestres, quanto em squamatas, porém há a escassez de relatos em tartarugas marinhas documentados até o momento. Desta forma, objetivou-se relatar o processo de reabilitação de uma tartaruga-verde (Chelonia mydas) com prolapso peniano, de modo a nortear procedimentos clínicos e terapêuticos que possam propiciar maior sucesso na reabilitação destes animais. Uma tartaruga-verde (C. mydas), adulto, macho, foi resgatada no dia 02 de maio de 2020 na praia de Muriú, Ceará Mirim, Rio Grande do Norte, Brasil. O espécime encontrava-se ativo, responsivo, com bom escore corpóreo, hidratação moderada, presença de prolapso peniano com mucosa associada hiperêmica e parte necrosada, além disso, encontrava-se com a nadadeira anterior esquerda amputada. O animal recebeu suporte terapêutico à base de analgésicos, antibióticos, anti-inflamatórios, polivitamínicos e fluidoterapia. Foram realizados procedimentos cirúrgicos para remoção total do úmero e redução mecânica do prolapso peniano. Após 50 dias de reabilitação o animal teve alta médica e foi reintroduzido de volta ao mar. Conclui-se que o protocolo utilizado, tanto na reabilitação, quanto no tratamento conservativo do prolapso peniano mostrou-se eficaz na redução do edema, evitando assim a recidiva do prolapso.(AU)

The occurrence of penile prolapse in reptiles has been observed both in terrestrial turtles and squamates. However, there are few reports of penile prolapse in sea turtles published to date. This study aimed at reporting the rehabilitation process of a green sea turtle (Chelonia mydas) with penile prolapse, in order to guide clinical and therapeutic measures that can provide greater success in the rehabilitation of these animals. An adult male green sea turtle (C. mydas) was rescued on May 2, 2020 at Muriú beach, Ceará Mirim, Rio Grande do Norte, Brazil. The specimen was active and responsive, with a good body score and moderate hydration, and showed the presence of penile prolapse with associated hyperemic mucosa and necrosis. It also had the left anterior fin amputated. The animal received therapeutic support based on antibiotics, analgesics, anti-inflammatories, multivitamins, and fluid therapy. The surgical procedure for the complete removal of the humerus and mechanical reduction of penile prolapse was performed. After 50 days of rehabilitation, the animal was discharged and was reintroduced back to the sea. It is concluded that the protocol used was effective in reducing edema, thus preventing the recurrence of prolapse, both in rehabilitation and in the conservative treatment of penile prolapse(AU)

Prolapso peniano em tartaruga-verde (Chelonia mydas) / Penile prolapse in green sea turtle (Chelonia mydas)

A ocorrência de prolapso peniano em repteis é observada tanto em quelônios terrestres, quanto em squamatas, porém há a escassez de relatos em tartarugas marinhas documentados até o momento. Desta forma, objetivou-se relatar o processo de reabilitação de uma tartaruga-verde (Chelonia mydas) com prolapso peniano, de modo a nortear procedimentos clínicos e terapêuticos que possam propiciar maior sucesso na reabilitação destes animais. Uma tartaruga-verde (C. mydas), adulto, macho, foi resgatada no dia 02 de maio de 2020 na praia de Muriú, Ceará Mirim, Rio Grande do Norte, Brasil. O espécime encontrava-se ativo, responsivo, com bom escore corpóreo, hidratação moderada, presença de prolapso peniano com mucosa associada hiperêmica e parte necrosada, além disso, encontrava-se com a nadadeira anterior esquerda amputada. O animal recebeu suporte terapêutico à base de analgésicos, antibióticos, anti-inflamatórios, polivitamínicos e fluidoterapia. Foram realizados procedimentos cirúrgicos para remoção total do úmero e redução mecânica do prolapso peniano. Após 50 dias de reabilitação o animal teve alta médica e foi reintroduzido de volta ao mar. Conclui-se que o protocolo utilizado, tanto na reabilitação, quanto no tratamento conservativo do prolapso peniano mostrou-se eficaz na redução do edema, evitando assim a recidiva do prolapso.

The occurrence of penile prolapse in reptiles has been observed both in terrestrial turtles and squamates. However, there are few reports of penile prolapse in sea turtles published to date. This study aimed at reporting the rehabilitation process of a green sea turtle (Chelonia mydas) with penile prolapse, in order to guide clinical and therapeutic measures that can provide greater success in the rehabilitation of these animals. An adult male green sea turtle (C. mydas) was rescued on May 2, 2020 at Muriú beach, Ceará Mirim, Rio Grande do Norte, Brazil. The specimen was active and responsive, with a good body score and moderate hydration, and showed the presence of penile prolapse with associated hyperemic mucosa and necrosis. It also had the left anterior fin amputated. The animal received therapeutic support based on antibiotics, analgesics, anti-inflammatories, multivitamins, and fluid therapy. The surgical procedure for the complete removal of the humerus and mechanical reduction of penile prolapse was performed. After 50 days of rehabilitation, the animal was discharged and was reintroduced back to the sea. It is concluded that the protocol used was effective in reducing edema, thus preventing the recurrence of prolapse, both in rehabilitation and in the conservative treatment of penile prolapse

Doenças infecciosas e não infecciosas nos peixes-boi do Brasil / Infectious and non-infectious diseases in manatees in Brazil

Background: Antillean manatee (Trichechus manatus) and Amazonian manatee (Trichechus inunguis) are the two species of manatees that occur in Brazil, and both are endangered. Studies on manatee disease in Brazil were previously based mainly on Florida manatees. In recent years, these studies have increased in Brazil. Thus, the objective was to carry out a survey of the publications of infectious and non-infectious diseases in the two species of manatees that occur in the country, to assist in the identification of threats to the species and in the health assessment of manatees in the country. Review: Regarding infectious and contagious diseases, parasites have affected both species of manatees in Brazil, such as Pulmonicola cochleotrema in wild T. manatus, Toxoplasma gondii in both species, and Cryptosporidium spp. also in both species, this being an important agent of zoonotic character. Giardia sp., another zoonotic parasite, has also been reported in both species of manatees in Brazil. Mycotic lesions caused by B. hawaiiensis have been described in calves of T. manatus in captivity. Meanwhile, Candida sp., Trichosporon sp., Rhodotorula sp., and Cryptococcus sp. have been identified in T. inunguis. Studies on viruses in manatees are still not widespread compared to other species; however, it has been verified that rotavirus is present in T. manatus in captivity. In studies carried out to identify bacteria in T. manatus, the following bacteria were isolated: Arizona sp., Bacillus sp., Corynebacterium equi, Corynebacterium pseudodiphteriticum, Corinebacterium sp., Edwasiella tarda, Enterobacter aerogenes, Enterobacter agglomerans, Enterobacter sakasakii, Escherichia coli, Leptospira spp., Proteus sp., Proteus vulgaris, Providencia rettgeri...

Piotórax em peixe-boi-marinho (Trichechus manatus) no Brasil / Pyothorax in Antillean manatee (Trichechus manatus) in Brazil

Background: Manatees are the most endangered aquatic mammals in Brazil. The current conservation scenario, together with their biological characteristics, raises concern with the future of this species. Pyothorax, also known as septic pleural effusion or pleural empyema, is characterized by the accumulation of a septic purulent exudate within the pleural space. Although this infection often has a multifactorial etiology, it is most commonly associated with respiratory tract disorders and trauma. Here, we report a case of pyothorax in a Antillean manatee (Trichechus manatus) held in captivity for acclimatization in Brazil. Case: A young, male Antillean manatee, aged 4 years and 11 months, measuring 227 cm in total length, and weighing 258 kg was held in captivity for acclimatization (natural environment) in Porto de Pedras in the State of Alagoas, Brazil. The animal died in February 2013 and was sent for necropsy at the CMA/ICMBio laboratory. The study was developed with authorisation from the Ethics Committee on Animal Use (License number 020/2009) and under the license SISBIO/ ICMBio number 20685-1. Externally, the carcass of the animal showed swelling and bulging of the right antimere and purulent secretion from the right nostril. After external examination, a window was opened in the layers of skin, fat, and muscles from the level of the anus to the navel. Subsequently, the abdominal cavity was opened for inspecting the internal organs. The subcutaneous muscles had a slightly friable, swollen, and pale texture, in addition to petechial hemorrhage, suffusions, and marked edema of the subcutaneous tissue. The right hemidiaphragm was elevated, filling the abdominal and thoracic spaces. Depending on the elevation, all organs were moved to...

Piotórax em peixe-boi-marinho (Trichechus manatus) no Brasil / Pyothorax in Antillean manatee (Trichechus manatus) in Brazil

Background: Manatees are the most endangered aquatic mammals in Brazil. The current conservation scenario, together with their biological characteristics, raises concern with the future of this species. Pyothorax, also known as septic pleural effusion or pleural empyema, is characterized by the accumulation of a septic purulent exudate within the pleural space. Although this infection often has a multifactorial etiology, it is most commonly associated with respiratory tract disorders and trauma. Here, we report a case of pyothorax in a Antillean manatee (Trichechus manatus) held in captivity for acclimatization in Brazil. Case: A young, male Antillean manatee, aged 4 years and 11 months, measuring 227 cm in total length, and weighing 258 kg was held in captivity for acclimatization (natural environment) in Porto de Pedras in the State of Alagoas, Brazil. The animal died in February 2013 and was sent for necropsy at the CMA/ICMBio laboratory. The study was developed with authorisation from the Ethics Committee on Animal Use (License number 020/2009) and under the license SISBIO/ ICMBio number 20685-1. Externally, the carcass of the animal showed swelling and bulging of the right antimere and purulent secretion from the right nostril. After external examination, a window was opened in the layers of skin, fat, and muscles from the level of the anus to the navel. Subsequently, the abdominal cavity was opened for inspecting the internal organs. The subcutaneous muscles had a slightly friable, swollen, and pale texture, in addition to petechial hemorrhage, suffusions, and marked edema of the subcutaneous tissue. The right hemidiaphragm was elevated, filling the abdominal and thoracic spaces. Depending on the elevation, all organs were moved to...(AU)

Artérias colaterais do arco aórtico do gerbil (Meriones unguiculatus) / Collateral arteries of the aortic arch in mongolian gerbil (meriones unguiculatus)

Background: Gerbils (Meriones unguiculatus) are rodents belonging to the Muridae family. Recently, breeding of this species as pets has increased significantly. Animal models are being investigated to study diseases related to the human aortic arch. Despite the importance of the aortic arch in maintaining homeostasis, there is limited data available regarding its morphology in gerbils. This study was performed with the objective of describing the collateral branches of the aortic arch in this animal to establish a standard model and thus contribute to future research on cardiovascular diseases in humans.Materials, Methods & Results: This study used 20 male specimens from previous studies that were frozen and stored at the Laboratory of Veterinary Anatomy of the Federal Rural University of the Semi-Arid Region. After thawing the animals, the thoracic cavity was opened for aortic cannulation. The vascular system was washed using saline solution and Neoprene latex stained with red pigment was injected. Subsequently, the animals were fixed in 10% formaldehyde and were dissected and analyzed 72 h later. The arrangement of the collateral branches of the aortic arch in gerbils was analyzed in all animals. The brachiocephalic trunk, the left common carotid, and the left subclavian artery were observed to originate as collateral branches. The brachiocephalic trunk bifurcated into the right common carotid and the right subclavian arteries. The right and the left subclavian arteries branched into the vertebral artery, the internal thoracic artery, the superficial cervical artery, the costocervical trunk, and the axillary artery.[...]

Artérias colaterais do arco aórtico do gerbil (Meriones unguiculatus) / Collateral arteries of the aortic arch in mongolian gerbil (meriones unguiculatus)

Background: Gerbils (Meriones unguiculatus) are rodents belonging to the Muridae family. Recently, breeding of this species as pets has increased significantly. Animal models are being investigated to study diseases related to the human aortic arch. Despite the importance of the aortic arch in maintaining homeostasis, there is limited data available regarding its morphology in gerbils. This study was performed with the objective of describing the collateral branches of the aortic arch in this animal to establish a standard model and thus contribute to future research on cardiovascular diseases in humans.Materials, Methods & Results: This study used 20 male specimens from previous studies that were frozen and stored at the Laboratory of Veterinary Anatomy of the Federal Rural University of the Semi-Arid Region. After thawing the animals, the thoracic cavity was opened for aortic cannulation. The vascular system was washed using saline solution and Neoprene latex stained with red pigment was injected. Subsequently, the animals were fixed in 10% formaldehyde and were dissected and analyzed 72 h later. The arrangement of the collateral branches of the aortic arch in gerbils was analyzed in all animals. The brachiocephalic trunk, the left common carotid, and the left subclavian artery were observed to originate as collateral branches. The brachiocephalic trunk bifurcated into the right common carotid and the right subclavian arteries. The right and the left subclavian arteries branched into the vertebral artery, the internal thoracic artery, the superficial cervical artery, the costocervical trunk, and the axillary artery.[...](AU)

Vascularização arterial da base do encéfalo de preá (Galea spixii) / Arterial vascularization of the base of the brain in galea (Galea spixii)

Background: Galea (Galea spixii) are rodents which are especially distributed in the northeastern region of Brazil, and have economic importance as their meat is used as a source of protein by the regional population. Currently, they have received attention from researchers in studies involving their morphology. Thus, seeking to contribute information that supports their sanitary management in addition to the lack of literature on their nervous system, our objective was to describe the arterial vascularization of the base of the brain in this species, in order to identify the arterial pattern and arterial circuit behavior, as well as possible variations in these vessels. Materials, Methods & Results: Brains were obtained from 20 animals (10 males and 10 females) aged between 11 and 12 months, obtained from the Wild Animal Multiplication Center (CEMAS) of the Federal Rural Semi-Arid University (UFERSA), under the approval of CEUA/UFERSA (case number 23.091.000653/2014-26 and opinion number 15/2014), euthanized according to anesthetic protocol recommended for rodents (resolution number 714/2002 of the CFMV/ UFERSA) and preserved frozen in a freezer for an average period of 30 days. The animals were thawed and a longitudinal incision was performed in the thoracic region to allow exposure of the aortic arch. Next, they were cannulated in the cranial direction and injected with Noprene Latex “650” stained with water-based white or red pigment. The animals were subsequently fixed in 3.7% aqueous formaldehyde solution for 48 h, and soon after the brain skull cap was dissected and removed, which was then analyzed, photographed and sketched. Of the 20 animals evaluated, 100% presented vertebrobasilar system responsible for encephalic irrigation of the rostral and caudal regions, and anastomosis of the left internal carotid artery was found in only […]

Vascularização arterial da base do encéfalo de preá (Galea spixii) / Arterial vascularization of the base of the brain in galea (Galea spixii)

Background: Galea (Galea spixii) are rodents which are especially distributed in the northeastern region of Brazil, and have economic importance as their meat is used as a source of protein by the regional population. Currently, they have received attention from researchers in studies involving their morphology. Thus, seeking to contribute information that supports their sanitary management in addition to the lack of literature on their nervous system, our objective was to describe the arterial vascularization of the base of the brain in this species, in order to identify the arterial pattern and arterial circuit behavior, as well as possible variations in these vessels. Materials, Methods & Results: Brains were obtained from 20 animals (10 males and 10 females) aged between 11 and 12 months, obtained from the Wild Animal Multiplication Center (CEMAS) of the Federal Rural Semi-Arid University (UFERSA), under the approval of CEUA/UFERSA (case number 23.091.000653/2014-26 and opinion number 15/2014), euthanized according to anesthetic protocol recommended for rodents (resolution number 714/2002 of the CFMV/ UFERSA) and preserved frozen in a freezer for an average period of 30 days. The animals were thawed and a longitudinal incision was performed in the thoracic region to allow exposure of the aortic arch. Next, they were cannulated in the cranial direction and injected with Noprene Latex “650” stained with water-based white or red pigment. The animals were subsequently fixed in 3.7% aqueous formaldehyde solution for 48 h, and soon after the brain skull cap was dissected and removed, which was then analyzed, photographed and sketched. Of the 20 animals evaluated, 100% presented vertebrobasilar system responsible for encephalic irrigation of the rostral and caudal regions, and anastomosis of the left internal carotid artery was found in only […](AU)

Origem e distribuição da artéria celíaca em preás (Galea spixii Wagler, 1831) / Origin and distribution of the celiac artery in yellow-toothed cavies (Galea spixii Wagler, 1831)

Para o estudo foram utilizados 20 animais, que estavam em freezer no Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA). Os animais foram descongelados, a cavidade abdominal aberta e a aorta torácica canulada no sentido caudal para injeção de solução de látex Neoprene 650 corado de vermelho e, em seguida, fixados em solução de formaldeído a 10%, por um período mínimo de 48 horas, para que pudessem ser dissecados, fotografados e os resultados analisados. A artéria celíaca originou-se da aorta abdominal e emitiu as artérias gástrica esquerda, lienal e esplênica. A artéria gástrica esquerda emitiu de dois a três ramos à região da curvatura menor do estômago. A artéria lienal emitiu quatro ramos ao parênquima do baço, de seis a doze ramos pancreáticos e a artéria gastroepiplóica esquerda. A artéria hepática emitiu de um a dois ramos pancreáticos e bifurcou-se em um curto tronco formado pelas artérias gástrica direita e hepática própria e ainda na artéria gastroduodenal, que originava a pancreaticoduodenal cranial e gastroepiplóica direita. O comportamento de trifurcação da artéria celíaca do preá assemelha-se ao encontrado em roedores, como o nutria, a cutia, o hamster e os ratos, o que sugere que este seja o padrão para a ordem.(AU)

For this study we used 20 animals that were in the freezer at the Wild Animals Multiplication Center (CEMAS/UFERSA). The animals were thawed, the abdominal cavity was opened, and the thoracic aorta cannulated caudally for the injection of red-stained latex Neoprene 650, and then fixed in formaldehyde solution 10%, for a minimum of 48 hours. Then the animals were dissected, photographed, and the results analyzed. The celiac artery originated from the abdominal aorta and issued the left gastric, splenic, and hepatic arteries. The left gastric artery issued two to three branches to the lesser curvature of the stomach. The splenic artery emitted four branches to the parenchyma of the spleen, six to twelve pancreatic branches and the left gastroepiploic artery. The hepatic artery emitted from one to two pancreatic branches and forked in a short trunk formed by right gastric and own hepatic arteries and in the gastroduodenal artery, which originated the cranial pancreaticoduodenal and right gastroepiploic. The trifurcation behavior of the celiac artery of the yellow-toothed cavy is similar to that found in rodents such as nutria, agouti, hamsters and mice, suggesting that this is the standard for the order.(AU)

Origem e distribuição da artéria celíaca em preás (Galea spixii Wagler, 1831) / Origin and distribution of the celiac artery in yellow-toothed cavies (Galea spixii Wagler, 1831)

Para o estudo foram utilizados 20 animais, que estavam em freezer no Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA). Os animais foram descongelados, a cavidade abdominal aberta e a aorta torácica canulada no sentido caudal para injeção de solução de látex Neoprene 650 corado de vermelho e, em seguida, fixados em solução de formaldeído a 10%, por um período mínimo de 48 horas, para que pudessem ser dissecados, fotografados e os resultados analisados. A artéria celíaca originou-se da aorta abdominal e emitiu as artérias gástrica esquerda, lienal e esplênica. A artéria gástrica esquerda emitiu de dois a três ramos à região da curvatura menor do estômago. A artéria lienal emitiu quatro ramos ao parênquima do baço, de seis a doze ramos pancreáticos e a artéria gastroepiplóica esquerda. A artéria hepática emitiu de um a dois ramos pancreáticos e bifurcou-se em um curto tronco formado pelas artérias gástrica direita e hepática própria e ainda na artéria gastroduodenal, que originava a pancreaticoduodenal cranial e gastroepiplóica direita. O comportamento de trifurcação da artéria celíaca do preá assemelha-se ao encontrado em roedores, como o nutria, a cutia, o hamster e os ratos, o que sugere que este seja o padrão para a ordem.

For this study we used 20 animals that were in the freezer at the Wild Animals Multiplication Center (CEMAS/UFERSA). The animals were thawed, the abdominal cavity was opened, and the thoracic aorta cannulated caudally for the injection of red-stained latex Neoprene 650, and then fixed in formaldehyde solution 10%, for a minimum of 48 hours. Then the animals were dissected, photographed, and the results analyzed. The celiac artery originated from the abdominal aorta and issued the left gastric, splenic, and hepatic arteries. The left gastric artery issued two to three branches to the lesser curvature of the stomach. The splenic artery emitted four branches to the parenchyma of the spleen, six to twelve pancreatic branches and the left gastroepiploic artery. The hepatic artery emitted from one to two pancreatic branches and forked in a short trunk formed by right gastric and own hepatic arteries and in the gastroduodenal artery, which originated the cranial pancreaticoduodenal and right gastroepiploic. The trifurcation behavior of the celiac artery of the yellow-toothed cavy is similar to that found in rodents such as nutria, agouti, hamsters and mice, suggesting that this is the standard for the order.

ORIGEM E DISTRIBUIÇÃO DA ARTÉRIA CELÍACA EM PREÁS (Galea spixii Wagler, 1831)

Abstract For this study we used 20 animals that were in the freezer at the Wild Animals Multiplication Center (CEMAS/UFERSA). The animals were thawed, the abdominal cavity was opened, and the thoracic aorta cannulated caudally for the injection of red-stained latex Neoprene 650, and then fixed in formaldehyde solution 10%, for a minimum of 48 hours. Then the animals were dissected, photographed, and the results analyzed. The celiac artery originated from the abdominal aorta and issued the left gastric, splenic, and hepatic arteries. The left gastric artery issued two to three branches to the lesser curvature of the stomach. The splenic artery emitted four branches to the parenchyma of the spleen, six to twelve pancreatic branches and the left gastroepiploic artery. The hepatic artery emitted from one to two pancreatic branches and forked in a short trunk formed by right gastric and own hepatic arteries and in the gastroduodenal artery, which originated the cranial pancreaticoduodenal and right gastroepiploic. The trifurcation behavior of the celiac artery of the yellow-toothed cavy is similar to that found in rodents such as nutria, agouti, hamsters and mice, suggesting that this is the standard for the order.

Resumo Para o estudo foram utilizados 20 animais, que estavam em freezer no Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA). Os animais foram descongelados, a cavidade abdominal aberta e a aorta torácica canulada no sentido caudal para injeção de solução de látex Neoprene 650 corado de vermelho e, em seguida, fixados em solução de formaldeído a 10%, por um período mínimo de 48 horas, para que pudessem ser dissecados, fotografados e os resultados analisados. A artéria celíaca originou-se da aorta abdominal e emitiu as artérias gástrica esquerda, lienal e esplênica. A artéria gástrica esquerda emitiu de dois a três ramos à região da curvatura menor do estômago. A artéria lienal emitiu quatro ramos ao parênquima do baço, de seis a doze ramos pancreáticos e a artéria gastroepiplóica esquerda. A artéria hepática emitiu de um a dois ramos pancreáticos e bifurcou-se em um curto tronco formado pelas artérias gástrica direita e hepática própria e ainda na artéria gastroduodenal, que originava a pancreaticoduodenal cranial e gastroepiplóica direita. O comportamento de trifurcação da artéria celíaca do preá assemelha-se ao encontrado em roedores, como o nutria, a cutia, o hamster e os ratos, o que sugere que este seja o padrão para a ordem.

Vascularização arterial da base do encéfalo do gerbil (Meriones unguiculatus Milne-Edwards, 1867) / Arterial vascularization of the base of the cerebrum of mongolian gerbils(Meriones unguiculatus Milne-Edwards, 1867)

Objetivou-se descrever e sistematizar as artérias da base do encéfalo. Foram utilizados dez gerbils que se encontravamarmazenados em freezer no laboratório de Morfofisiologia Animal Aplicada da Universidade Federal Rural do Semiárido. Osanimais foram descongelados, incisados na linha mediana para identificação do ventrículo esquerdo, o qual foi perfurado einjetados 3ml de Látex Neoprene 650 na concentração de 20% e no pigmento vermelho. Posteriormente aguardou-se cerca de 1minuto em virtude da polimerização do Látex e logo após foram fixados por imersão em solução aquosa de Formaldeído a 3,7% eapós 72 horas, tiveram os encéfalos removidos da calota craniana para análise de sua superfície ventral. Foi visto que o sistemavertebrobasilar e carótico estiveram presentes em todos os animais analisados, mostrando-se responsável pela vascularizaçãodo encéfalo caudal e rostral, respectivamente. As principais artérias observadas no modelo padrão incluem as ímpares: basilar,espinhal ventral, ramo medial da artéria cerebral rostral e as pares: vertebral, cerebelar caudal, cerebelar média, trigeminal,cerebelar rostral, ramo terminal da basilar, carótida interna, cerebral caudal, hipofisária, cerebral média, cerebral rostral, lateral dobulbo olfatório e etmoidal interna. Conclui-se com base nos resultados que a irrigação da superfície ventral encefálica do gerbilfoi suprida pelos sistemas vertebro-basilar e carótico de forma equivalente, pertencendo assim ao modelo do tipo II. O circuitoarterioso apresentou-se aberto caudalmente e fechado rostralmente, fato que difere do relatado em roedores como capivara,cutia, preá, chinchila e nutria.(AU)

The objective was to describe and systematize the arteries of the base of the brain. Ten gerbils were used, which were stored in afreezer in the Laboratory of Applied Animal Morphophysiology of the Federal Rural Semi-Arid University. The animals were thawed,incised in the midline to identify the left ventricle, which was punctured and injected 3 ml of Latex Neoprene 650 at a concentrationof 20% and red pigment. After about one minute the polymerization of the Latex was delayed and afterwards fixed by immersionin 3.7% aqueous Formaldehyde solution and after 72 hours the brain was removed from the skull cap for analysis of its ventralsurface. It was observed that the vertebro-basilar and carotid system were present in all animals analyzed, being responsible forthe vascularization of the caudal and rostral encephalon, respectively. The main arteries observed in the standard model includethe odd: basilar, ventral spinal, medial branch of the rostral cerebral artery and, the pairs: vertebral, cerebellar caudal, cerebellarmedial, trigeminal, cerebellar rostral, terminal branch of the basilar, internal carotid, caudal cerebral, Pituitary, middle cerebral, rostral,lateral, olfactory and internal ethmoidal. Based on the results, the irrigation of the ventral brain surface of the gerbil was suppliedby the vertebro-basilar and carotic systems in an equivalent way, thus belonging to the type II model. The arterial circuit was opencaudally and closed rostrally, a fact that differs from that reported in rodents such as capybara, agouti, cavy, chinchilla and nutria.(AU)

Arterial vascularization of the brain of the agouti (Dasyprocta aguti Linnaeus, 1766) / Vascularização arterial da base do encéfalo de cutias (Dasyprocta aguti Linnaeus, 1766)

The agouti, a rodent that is geographically distributed throughout South America, is greatly valued for its meat. This paper describes the arterial vascularization of the base of the agoutis brain, characterizing behavior, and arterial origin and distribution. Ten animals from the Center for the Multiplication of Wild Animals (CEMAS/UFERSA) were used and the study was approved by SISBIO (report number 32413-1) and the Ethics Committee on Animal Use (CEUA/UFERSA) (protocol 02/2010). After euthanasia, the animals were incised in the thoracic cavity by an injection of red-stained Neoprene latex 650 and the skulls were subsequently opened. The brains were extracted from the skulls for ventral surface analysis and then fixed in an aqueous 10% formaldehyde solution. The agoutis arterial vascularization of the brain has two main components, namely the carotid and vertebrobasilar systems. The agoutis carotid system accounts for vascularization of almost the entire forebrain, while the vertebrobasilar system accounts for vascularization of almost the entire posterior brain (medulla oblongata, pyramid, trapezoid body, cerebellum, bridge, and part of the third caudal section of the forebrain) through the caudal cerebral arteries originating from the terminal branches of the basilar artery. The main arteries on the brain surface include the basilar artery, which is unique, and the arterial pairs, specifically the vertebral arteries, cerebellar caudal arteries, trigeminal artery, rostral cerebellar artery, basilar terminal branch artery, cerebral caudal artery, communicating caudal branch of the cerebral carotid artery, cerebral carotid artery, communicating branch rostral cerebral carotid artery, choroidal rostral artery, medial branch of the communicating branch rostral artery, internal ophthalmic artery, middle cerebral artery, and rostral cerebral artery.(AU)

A cutia é um roedor, que se distribui geograficamente por quase toda América Latina, sendo apreciada pelo sabor da sua carne. Neste trabalho objetivou-se descrever a vascularização arterial da base do encéfalo de cutias, caracterizando o comportamento, origem e distribuição das artérias componentes. Foram utilizados 10 animais do Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA), pesquisa aprovada pela Comissão de Ética no Uso de Animais (CEUA/ UFERSA- n° 02/2010) e pelo SISBIO (N°32413-1). Após eutanásia, os animais foram incisados na cavidade torácica para injeção de Neoprene látex 650 corado na cor vermelha e posterior abertura da calota craniana. Os encéfalos foram extraídos do crânio para análise da sua face ventral e logo fixados em solução aquosa de formaldeído a 10%. A vascularização arterial do encéfalo da cutia apresenta dois componentes principais, o sistema carótico e o vértebro-basilar. O sistema carótico na cutia é responsável pela vascularização do cérebro anterior em quase sua totalidade. O sistema vértebro-basilar é responsável pela vascularização do cérebro posterior (medula oblonga, pirâmide, corpo trapezóide, o cerebelo e ponte e parte do terço caudal do cérebro anterior), através das artérias cerebrais caudais, originárias dos ramos terminais da artéria basilar. As principais artérias presentes na superfície do encéfalo incluem a basilar que é ímpar, e as pares: artérias vertebrais, cerebelar caudal, trigemial, cerebelar rostral, ramo terminal da basilar, cerebral caudal, ramo comunicante caudal da carótida cerebral, carótida cerebral, ramo comunicante rostral da carótida cerebral, corióidearostral, ramo medial do ramo comunicante rostral, oftálmica interna, cerebral média e artéria cerebral rostral.(AU)

The lumbosacral plexus of the red-rumped agouti (Dasyprocta leporina Linnaeus, 1758) (Rodentia: Caviidae) / Plexo lombossacral da cutia (Dasyprocta leporina Linnaeus, 1758) (Rodentia: Caviidae)

The red-rumped agouti, a small, wild rodent belonging to the Dasyproctidae family, has greatzootechnical potential and adapts well to captivity. In order to contribute to the knowledge regarding the neuroanatomy of the species, the aim of this study was to describe the origin of the nerves forming the lumbosacral plexus by evaluating 12 animals (six males and six females) that had been used in previous experiments. Animals were fixed in a 10% aqueous formaldehyde solution and eviscerated after 72 hours. Major and minor psoas muscles were then retracted to expose the nerves forming the plexus, and the plexus was bleached via cotton soaked in 20-volume hydrogen peroxide for 12 hours for subsequent dissection. Topographical relations of the lumbosacral plexus were grouped into tables and arranged in terms of simple percentages. In seven cases (58.34%), the lumbosacral plexus in the red-rumped agouti stemmed from the ventral roots of the last four lumbar nerves and the first three sacral nerves (Type I: L4-S3). In four animals (33.33%), the lumbosacral plexus stemmed from L5-S3 (Type II), and in one case (8.33%) it stemmed from L5-S4 (Type III). Together, our observations demonstrated that thelumbosacral plexus of the red-rumped agouti comprised the lateral femoral cutaneous, genitofemoral, femoral, obturator, sciatic, cranial gluteal, caudal gluteal, and pudendal nerves. Finally, the origin of the lumbosacral plexus and its constituent spinal nerves was similar to what has been described in other rodents, including the rock cavy, lowland paca, and Spix’s yellow-toothed cavy.

A cutia é um roedor silvestre, de pequeno porte pertencente à família Dasyproctidae, com grandepotencial zootécnico e que se adapta bem ao cativeiro. De forma a contribuir acerca da biologia daespécie, objetivou-se descrever a origem dos nervos que formam o plexo lombossacral. Foram utilizados 12 animais (6 machos e 6 fêmeas), provenientes de experimentos anteriores. Os animais foram fixados em solução aquosa de formaldeído a 10% e após 72 horas, eviscerados. Em seguida os músculos psoas maior e menor foram rebatidos, expondo-se os nervos que formavam o plexo. Sobre estes nervos foi colocado algodão embebido com peróxido de hidrogênio a 20 volumes, permanecendo por 12 horas seguidas para clareamento e consequente dissecação. As relações topográficas do plexo lombossacral agrupadas em tabelas em forma de porcentagem simples. O plexo lombossacral da cutia originou-se em sete casos (58,34%) a partir das raízes ventrais dos quatro últimos nervos lombares e três primeiros nervos sacrais (Tipo I - L4-S3), em quatro animais (33,33%) a partir de L5-S3 (Tipo II) e um caso (8,33%) a partir de L5-S4 (Tipo III). Os nervos que participaram do plexo lombossacral da cutia foram: cutâneo femoral lateral, genitofemoral, femoral, obturatório, isquiático, glúteo cranial, glúteo caudal e nervo pudendo. A origem do plexo lombossacral e os nervos espinhais constituintes deste plexo em cutias foram semelhantes ao descrito em alguns roedores como mocó, paca e preá.

The lumbosacral plexus of the red-rumped agouti (Dasyprocta leporina Linnaeus, 1758) (Rodentia: Caviidae) / Plexo lombossacral da cutia (Dasyprocta leporina Linnaeus, 1758) (Rodentia: Caviidae)

The red-rumped agouti, a small, wild rodent belonging to the Dasyproctidae family, has greatzootechnical potential and adapts well to captivity. In order to contribute to the knowledge regarding the neuroanatomy of the species, the aim of this study was to describe the origin of the nerves forming the lumbosacral plexus by evaluating 12 animals (six males and six females) that had been used in previous experiments. Animals were fixed in a 10% aqueous formaldehyde solution and eviscerated after 72 hours. Major and minor psoas muscles were then retracted to expose the nerves forming the plexus, and the plexus was bleached via cotton soaked in 20-volume hydrogen peroxide for 12 hours for subsequent dissection. Topographical relations of the lumbosacral plexus were grouped into tables and arranged in terms of simple percentages. In seven cases (58.34%), the lumbosacral plexus in the red-rumped agouti stemmed from the ventral roots of the last four lumbar nerves and the first three sacral nerves (Type I: L4-S3). In four animals (33.33%), the lumbosacral plexus stemmed from L5-S3 (Type II), and in one case (8.33%) it stemmed from L5-S4 (Type III). Together, our observations demonstrated that thelumbosacral plexus of the red-rumped agouti comprised the lateral femoral cutaneous, genitofemoral, femoral, obturator, sciatic, cranial gluteal, caudal gluteal, and pudendal nerves. Finally, the origin of the lumbosacral plexus and its constituent spinal nerves was similar to what has been described in other rodents, including the rock cavy, lowland paca, and Spixs yellow-toothed cavy.(AU)

A cutia é um roedor silvestre, de pequeno porte pertencente à família Dasyproctidae, com grandepotencial zootécnico e que se adapta bem ao cativeiro. De forma a contribuir acerca da biologia daespécie, objetivou-se descrever a origem dos nervos que formam o plexo lombossacral. Foram utilizados 12 animais (6 machos e 6 fêmeas), provenientes de experimentos anteriores. Os animais foram fixados em solução aquosa de formaldeído a 10% e após 72 horas, eviscerados. Em seguida os músculos psoas maior e menor foram rebatidos, expondo-se os nervos que formavam o plexo. Sobre estes nervos foi colocado algodão embebido com peróxido de hidrogênio a 20 volumes, permanecendo por 12 horas seguidas para clareamento e consequente dissecação. As relações topográficas do plexo lombossacral agrupadas em tabelas em forma de porcentagem simples. O plexo lombossacral da cutia originou-se em sete casos (58,34%) a partir das raízes ventrais dos quatro últimos nervos lombares e três primeiros nervos sacrais (Tipo I - L4-S3), em quatro animais (33,33%) a partir de L5-S3 (Tipo II) e um caso (8,33%) a partir de L5-S4 (Tipo III). Os nervos que participaram do plexo lombossacral da cutia foram: cutâneo femoral lateral, genitofemoral, femoral, obturatório, isquiático, glúteo cranial, glúteo caudal e nervo pudendo. A origem do plexo lombossacral e os nervos espinhais constituintes deste plexo em cutias foram semelhantes ao descrito em alguns roedores como mocó, paca e preá.(AU)

Cranial and caudal mesenteric arteries in the yellow-toothed cavy (Galea spixii) / Artérias mesentéricas cranial e caudal do preá (Galea spixii)

Background: The yellow-toothed cavy is a wild rodent in the Caviidae family. It adapts well to captivity and may, in thenear future, be bred on a large scale as an alternative source of low cost protein of high nutritional value. Considering thelack of information available on the vascularization of this species digestive system, and with the aim of contributing tothe body of knowledge of this species biology, this study describes the origin and distribution of the cranial and caudalmesenteric arteries of the yellow-toothed cavy.Materials, Methods & Results: Twenty male yellow-toothed cavies, previously used in other experiments (CEUA N.15/2014 and Process N° 23.091.000653/2014-26), were kept in a freezer at the Center for the Multiplication of WildAnimals (CEMAS / UFERSA). The animals were thawed, after which the thoracic aorta of each specimen was caudallycannulated and injected with red- or yellow-colored latex solution. The animals were then fixed in 10% formaldehydesolution, and dissected after 48 h, during which the branches of the cranial and caudal mesenteric arteries were described.In all cases, the cranial mesenteric artery arised from the section of abdominal aorta, at the section between the first andsecond lumbar vertebra, near the celiac artery, and it includes the following arterial branches: the caudal pancreatic duodenal, middle colic, duodenojejunal and right colic arteries, as well as eight to eleven jejune arteries, and the ileocecocolictrunk, from which extend one or two colic branches, five to seven cecum branches, and the ileal artery. In all animals, thecranial mesenteric artery originates from the abdominal aorta, posterior to the testicular arteries...(AU)

Cranial and caudal mesenteric arteries in the yellow-toothed cavy (Galea spixii) / Artérias mesentéricas cranial e caudal do preá (Galea spixii)

Background: The yellow-toothed cavy is a wild rodent in the Caviidae family. It adapts well to captivity and may, in thenear future, be bred on a large scale as an alternative source of low cost protein of high nutritional value. Considering thelack of information available on the vascularization of this species’ digestive system, and with the aim of contributing tothe body of knowledge of this species’ biology, this study describes the origin and distribution of the cranial and caudalmesenteric arteries of the yellow-toothed cavy.Materials, Methods & Results: Twenty male yellow-toothed cavies, previously used in other experiments (CEUA N.15/2014 and Process N° 23.091.000653/2014-26), were kept in a freezer at the Center for the Multiplication of WildAnimals (CEMAS / UFERSA). The animals were thawed, after which the thoracic aorta of each specimen was caudallycannulated and injected with red- or yellow-colored latex solution. The animals were then fixed in 10% formaldehydesolution, and dissected after 48 h, during which the branches of the cranial and caudal mesenteric arteries were described.In all cases, the cranial mesenteric artery arised from the section of abdominal aorta, at the section between the first andsecond lumbar vertebra, near the celiac artery, and it includes the following arterial branches: the caudal pancreatic duodenal, middle colic, duodenojejunal and right colic arteries, as well as eight to eleven jejune arteries, and the ileocecocolictrunk, from which extend one or two colic branches, five to seven cecum branches, and the ileal artery. In all animals, thecranial mesenteric artery originates from the abdominal aorta, posterior to the testicular arteries...