Contaminação do solo por ovos de geohelmintos com potencial zoonótico na cidade de Bom Jesus, Piauí, Brasil / Soil contamination by geohelminth eggs with zoonotic potential in the city of Bom Jesus, Piauí, Brazil

O objetivo deste trabalho foi pesquisar a presença de ovos de parasitas gastrointestinais de animais em solos de escolas municipais e de áreas públicas da cidade de Bom Jesus/Piauí. Foram coletadas amostras de solo de quatro escolas municipais, nas áreas de recreação, e de quatro locais públicos da cidade e processadas mediante a técnica de centrífugo-flutuação, utilizando a solução de sulfato de zinco a 33%. Das 49 amostras de solos analisadas, todas apresentaram-se positivas. Foi observada nos solos das áreas de recreação das escolas uma contaminação média de 73,45% para Toxocara spp. e 26,54% para Ancylostoma spp., já nas áreas públicas da cidade foi observada uma frequência média de 32,65% para Toxocara spp., 31,81% para Ancylostoma spp., 33,13% para Trichuris spp. e um único local com 9,25% para Dipylidium spp. Os resultados demonstraram que existe a exposição de pessoas a ovos de helmintos com potencial zoonótico em áreas de recreação nas escolas municipais e em áreas públicas de Bom Jesus/PI, Brasil.

The objective of this study was to investigate the presence of gastrointestinal parasites in soil from municipal schools and public areas in the city of Bom Jesus/Piauí. Soil samples were collected from the recreation areas of four municipal schools, and four public places in the city and processed using the centrifugal-flotation technique with the 33% zinc sulfate solution. All 49 soil samples analyzed were. An average contamination of 73.45% for Toxocara spp., and 26.54% for Ancylostoma spp., was observed in the soils of recreation areas of the schools, and in public areas of the city an average frequency of 32.65% for Toxocara spp., 31.81% for Ancylostoma spp., 33.13%, for Trichuris spp. and one place with 9.25% for Dipylidium spp. The results showed that people are exposed to helminth eggs with zoonotic potential in recreation areas in municipal schools and public areas in Bom Jesus/Piauí, Brazil.

Contaminação do solo por ovos de geohelmintos com potencial zoonótico na cidade de Bom Jesus, Piauí, Brasil / Soil contamination by geohelminth eggs with zoonotic potential in the city of Bom Jesus, Piauí, Brazil

O objetivo deste trabalho foi pesquisar a presença de ovos de parasitas gastrointestinais de animais em solos de escolas municipais e de áreas públicas da cidade de Bom Jesus/Piauí. Foram coletadas amostras de solo de quatro escolas municipais, nas áreas de recreação, e de quatro locais públicos da cidade e processadas mediante a técnica de centrífugo-flutuação, utilizando a solução de sulfato de zinco a 33%. Das 49 amostras de solos analisadas, todas apresentaram-se positivas. Foi observada nos solos das áreas de recreação das escolas uma contaminação média de 73,45% para Toxocara spp. e 26,54% para Ancylostoma spp., já nas áreas públicas da cidade foi observada uma frequência média de 32,65% para Toxocara spp., 31,81% para Ancylostoma spp., 33,13% para Trichuris spp. e um único local com 9,25% para Dipylidium spp. Os resultados demonstraram que existe a exposição de pessoas a ovos de helmintos com potencial zoonótico em áreas de recreação nas escolas municipais e em áreas públicas de Bom Jesus/PI, Brasil.(AU)

The objective of this study was to investigate the presence of gastrointestinal parasites in soil from municipal schools and public areas in the city of Bom Jesus/Piauí. Soil samples were collected from the recreation areas of four municipal schools, and four public places in the city and processed using the centrifugal-flotation technique with the 33% zinc sulfate solution. All 49 soil samples analyzed were. An average contamination of 73.45% for Toxocara spp., and 26.54% for Ancylostoma spp., was observed in the soils of recreation areas of the schools, and in public areas of the city an average frequency of 32.65% for Toxocara spp., 31.81% for Ancylostoma spp., 33.13%, for Trichuris spp. and one place with 9.25% for Dipylidium spp. The results showed that people are exposed to helminth eggs with zoonotic potential in recreation areas in municipal schools and public areas in Bom Jesus/Piauí, Brazil.(AU)

Bovine mastitis caused by multidrug-resistant Nocardia farcinica

Background: Mastitis caused by Nocardia is characterized by pyogranulomatous inflammation related to inadequatehygiene conditions and is difficult to treat. Prompted by the absence of documentation of Nocardia farcinica associated tobovine mastitis in the Northeast region of Brazil, this is the first report to describe bovine mastitis caused by multidrugresistant N. farcinica.Case: Four milk samples (one from each teat) obtained from a 3-year-old Jersey cow raised on a property located in the metropolitan region of Recife, Pernambuco state, Brazil, were submitted to the Laboratory of Infectious-Contagious Diseases ofthe Veterinary Hospital at Universidade Federal Rural de Pernambuco [UFRPE]. At the laboratory, samples were cultured inbase agar enriched with 7% sheep blood (blood agar) in a microbiological incubator at 37°C under aerobic conditions for 72h. After only 48 h, however, pure bacterial colony growth was observed in all samples. Macroscopic analysis revealed smallcolonies, with an irregular shape, dry aspect, and greyish in color. Gram-positive rods forming filaments and/or ramificationswere observed using a Gram staining method. Nocardia spp. were identified according to morphotinctorial characteristics.Susceptibility testing using the disc-diffusion method in agar (antibiogram) was performed using the following antibiotics:penicillin (10 IU), tetracycline (30 µg), amoxicillin (10 µg), gentamicin (10 µg), cephalexin (30 µg), erythromycin (15 µg),cephalothin (30 µg) and ampicillin (30 µg). However, the organism exhibited resistance to all drugs; as such, a new milksample was obtained at the same location the initial samples were collected. Samples (approximately 5 mL) were collectedaseptically and separately from all four teats in sterile bottles, during which the presence of granular material was noted.Bacterial culture was performed as...

Bovine mastitis caused by multidrug-resistant Nocardia farcinica

Background: Mastitis caused by Nocardia is characterized by pyogranulomatous inflammation related to inadequatehygiene conditions and is difficult to treat. Prompted by the absence of documentation of Nocardia farcinica associated tobovine mastitis in the Northeast region of Brazil, this is the first report to describe bovine mastitis caused by multidrugresistant N. farcinica.Case: Four milk samples (one from each teat) obtained from a 3-year-old Jersey cow raised on a property located in the metropolitan region of Recife, Pernambuco state, Brazil, were submitted to the Laboratory of Infectious-Contagious Diseases ofthe Veterinary Hospital at Universidade Federal Rural de Pernambuco [UFRPE]. At the laboratory, samples were cultured inbase agar enriched with 7% sheep blood (blood agar) in a microbiological incubator at 37°C under aerobic conditions for 72h. After only 48 h, however, pure bacterial colony growth was observed in all samples. Macroscopic analysis revealed smallcolonies, with an irregular shape, dry aspect, and greyish in color. Gram-positive rods forming filaments and/or ramificationswere observed using a Gram staining method. Nocardia spp. were identified according to morphotinctorial characteristics.Susceptibility testing using the disc-diffusion method in agar (antibiogram) was performed using the following antibiotics:penicillin (10 IU), tetracycline (30 µg), amoxicillin (10 µg), gentamicin (10 µg), cephalexin (30 µg), erythromycin (15 µg),cephalothin (30 µg) and ampicillin (30 µg). However, the organism exhibited resistance to all drugs; as such, a new milksample was obtained at the same location the initial samples were collected. Samples (approximately 5 mL) were collectedaseptically and separately from all four teats in sterile bottles, during which the presence of granular material was noted.Bacterial culture was performed as...(AU)

Cistite canina causada por Salmonella enterica subsp. enterica / Canine cystitis caused by Salmonella enterica subsp. enterica

Background: Urinary tract infection in dogs is usually associated with the presence of bacteria, with a higher prevalenceof Gram-negative bacteria, represented mainly by enteric bacteria such as Escherichia coli and Proteus spp., followed byGram-positive bacteria such as Staphylococcus spp., and Streptococcus spp. There are scant reports of Salmonella spp.as the causative agent of urinary tract infection in dogs. Indeed, the literature describes only a few cases, most of whichinvolve the isolation of these bacteria in feces. This paper reports a case of canine cystitis caused by Salmonella entericasubsp. enterica in the northeast region of Brazil.Case: A female dog of the Fila Brasileiro breed, about 9 year-old, wormed but unvaccinated, was evaluated at the Veterinary Hospital of the Federal University of Pernambuco - UFRPE. The dog showed clinical signs of apathy, cachexia,polyphagia, polyuria and opacity of the crystalline lens. The dog’s owner stated that the animal was fed with commercialdog food. In the clinical exam, the patient presented pale mucosa, cachexia, absence of ectoparasites, and her rectal temperature was 39.5°C. Moreover, cardiorespiratory auscultation of the patient revealed tachycardia (190 bpm) and tachypnea (36 bpm). The owner’s main complaint was the clinical condition of frequent urination (polyuria). A urinalysis andurine culture with antibiogram were requested as complementary exams, after collecting the urine by cystocentesis. Thevolume obtained in the physical examination of urinalysis was 7 mL of yellow urine with a putrid smell, cloudy appearance and density of 1.024. The chemical examination revealed pH 6.5, protein (+++), bilirubin (+), normal urobilinogenand negative reactions for glycoses, ketone, nitrite and urine occult blood. Bacteriuria and pyuria were detected in a urinesediment test. Urine was cultured on blood agar and Levine agar in a bacteriological incubator at 37°C under aerobiosis,for 24 h...

Cistite canina causada por Salmonella enterica subsp. enterica / Canine cystitis caused by Salmonella enterica subsp. enterica

Background: Urinary tract infection in dogs is usually associated with the presence of bacteria, with a higher prevalenceof Gram-negative bacteria, represented mainly by enteric bacteria such as Escherichia coli and Proteus spp., followed byGram-positive bacteria such as Staphylococcus spp., and Streptococcus spp. There are scant reports of Salmonella spp.as the causative agent of urinary tract infection in dogs. Indeed, the literature describes only a few cases, most of whichinvolve the isolation of these bacteria in feces. This paper reports a case of canine cystitis caused by Salmonella entericasubsp. enterica in the northeast region of Brazil.Case: A female dog of the Fila Brasileiro breed, about 9 year-old, wormed but unvaccinated, was evaluated at the Veterinary Hospital of the Federal University of Pernambuco - UFRPE. The dog showed clinical signs of apathy, cachexia,polyphagia, polyuria and opacity of the crystalline lens. The dogs owner stated that the animal was fed with commercialdog food. In the clinical exam, the patient presented pale mucosa, cachexia, absence of ectoparasites, and her rectal temperature was 39.5°C. Moreover, cardiorespiratory auscultation of the patient revealed tachycardia (190 bpm) and tachypnea (36 bpm). The owners main complaint was the clinical condition of frequent urination (polyuria). A urinalysis andurine culture with antibiogram were requested as complementary exams, after collecting the urine by cystocentesis. Thevolume obtained in the physical examination of urinalysis was 7 mL of yellow urine with a putrid smell, cloudy appearance and density of 1.024. The chemical examination revealed pH 6.5, protein (+++), bilirubin (+), normal urobilinogenand negative reactions for glycoses, ketone, nitrite and urine occult blood. Bacteriuria and pyuria were detected in a urinesediment test. Urine was cultured on blood agar and Levine agar in a bacteriological incubator at 37°C under aerobiosis,for 24 h...(AU)

Occurrence of anti-Neospora caninum antibodies in cattle in the dairy farming region of the state of Piauí, Brazil / Ocorrência de anticorpos anti- Neospora caninum em vacas na bacia leiteira do estado do Piauí, Brasil

This study focused on the detection of anti-Neospora caninum IgG antibodies in cows in the dairy farming region of the state of Piauí, Brazil. To this end, serum samples were collected from 255 dairy cows on 17 farms located in the dairy farming region of the municipality of Parnaíba, Piauí, Brazil. The indirect fluorescence antibody test (IFAT) was employed to detect anti-N. caninum IgG antibodies, using anti-bovine IgG (Sigma®) conjugated to fluorescein isothiocyanate (FITC) and a cutoff point of 1:200. Of the 255 samples analyzed, 69 (27.06%) were positive for anti- N. caninum IgG antibodies, the relative frequency found by property was: 1 (20.00%), 2 (53.33%), 3 (46.66%), 4 (53.33%), 5 (26.66%), 6 (6.66%), 7 (6.66%), 8 (20.00%), 9 (26.66%), 10 (26.66%), 11 (20.00%), 12 (20.00%), 13 (46.66%), 14 (26.66%), 15 (26.66%), 16 (20.00%) and 17 (13.33%). with titers of 200 (15.94%), 400 (20.30%), 800 (24.63%), 1600 (23.18%) and 3200 (15.94%), being the highest frequency for the titer of 800. This study demonstrates for the first time that cows from dairy herds of Parnaíba municipality, state of Piauí, are exposed to N. caninum.(AU)

Objetivou-se estudar a ocorrência de anticorpos IgG anti- Neospora caninum em vacas, na bacia leiteira do estado do Piauí, Brasil. Foram obtidas amostras séricas de 255 vacas leiteiras provenientes de 17 propriedades localizadas na bacia leiteira do município de Parnaíba, Piauí, Brasil. Para a pesquisa de anticorpos IgG anti- N. caninum foi empregada a técnica de Reação de Imunofluorescência Indireta, utilizando-se conjugado anti-IgG bovine (Sigma®) conjugado ao isotiocianato de fluoresceína e ponto de corte 1:200. Das 255 amostras analisadas, 69 (27,06%) foram positivas para anticorpos IgG anti- N. caninum, a frequência relativa encontrada por propriedade foi de: 1 (20,00%), 2 (53,33%), 3 (46,66%), 4 (53,33%), 5 (26,66%), 6 (6,66%), 7 (6,66%), 8 (20,00%), 9 (26,66%) 10 (26,66%), 11 (20,00%), 12 (20,00%), 13 (46,66%), 14 (26,66%), 15 (26,66%), 16 (20,00%) e 17 (13,33%) com títulos de 200 (15,94%), 400 (20,30%), 800 (24,63%), 1600 (23,18%) e 3200 (15,94%), sendo a maior frequência para o título de 800. Este estudo demonstra pela primeira vez que, vacas de rebanhos leiteiros do município de Parnaíba, estado do Piauí, estão expostas ao N. caninum.(AU)

Occurrence of anti-Neospora caninum antibodies in cattle in the dairy farming region of the state of Piauí, Brazil

Abstract This study focused on the detection of anti-Neospora caninum IgG antibodies in cows in the dairy farming region of the state of Piauí, Brazil. To this end, serum samples were collected from 255 dairy cows on 17 farms located in the dairy farming region of the municipality of Parnaíba, Piauí, Brazil. The indirect fluorescence antibody test (IFAT) was employed to detect anti-N. caninum IgG antibodies, using anti-bovine IgG (Sigma®) conjugated to fluorescein isothiocyanate (FITC) and a cutoff point of 1:200. Of the 255 samples analyzed, 69 (27.06%) were positive for anti- N. caninum IgG antibodies, the relative frequency found by property was: 1 (20.00%), 2 (53.33%), 3 (46.66%), 4 (53.33%), 5 (26.66%), 6 (6.66%), 7 (6.66%), 8 (20.00%), 9 (26.66%), 10 (26.66%), 11 (20.00%), 12 (20.00%), 13 (46.66%), 14 (26.66%), 15 (26.66%), 16 (20.00%) and 17 (13.33%). with titers of 200 (15.94%), 400 (20.30%), 800 (24.63%), 1600 (23.18%) and 3200 (15.94%), being the highest frequency for the titer of 800. This study demonstrates for the first time that cows from dairy herds of Parnaíba municipality, state of Piauí, are exposed to N. caninum.

Resumo Objetivou-se estudar a ocorrência de anticorpos IgG anti- Neospora caninum em vacas, na bacia leiteira do estado do Piauí, Brasil. Foram obtidas amostras séricas de 255 vacas leiteiras provenientes de 17 propriedades localizadas na bacia leiteira do município de Parnaíba, Piauí, Brasil. Para a pesquisa de anticorpos IgG anti- N. caninum foi empregada a técnica de Reação de Imunofluorescência Indireta, utilizando-se conjugado anti-IgG bovine (Sigma®) conjugado ao isotiocianato de fluoresceína e ponto de corte 1:200. Das 255 amostras analisadas, 69 (27,06%) foram positivas para anticorpos IgG anti- N. caninum, a frequência relativa encontrada por propriedade foi de: 1 (20,00%), 2 (53,33%), 3 (46,66%), 4 (53,33%), 5 (26,66%), 6 (6,66%), 7 (6,66%), 8 (20,00%), 9 (26,66%) 10 (26,66%), 11 (20,00%), 12 (20,00%), 13 (46,66%), 14 (26,66%), 15 (26,66%), 16 (20,00%) e 17 (13,33%) com títulos de 200 (15,94%), 400 (20,30%), 800 (24,63%), 1600 (23,18%) e 3200 (15,94%), sendo a maior frequência para o título de 800. Este estudo demonstra pela primeira vez que, vacas de rebanhos leiteiros do município de Parnaíba, estado do Piauí, estão expostas ao N. caninum.

Criptococose sistêmica em um canino da raça Miniatura Schnauzer / Systemic cryptococcosis in a Miniature Schnauzer dog

Background: Cryptococcosis is an important zoonotic disease that occurs worldwide. The disease is caused by a soilborne opportunistic fungus of the genus Cryptococcus, which can also be found in the feces of birds, especially pigeons. In Brazil, the geographical distribution of the agent is fairly defined, with the species C. gattii predominantly found in the north-eastern states. Diagnosis is based on the clinical history, physical examination findings, and results of complementary testing, such as cytopathological and histopathological examinations. This report aims to describe the clinical and anatomopathological findings in a case of systemic cryptococcosis in a dog.Case: A 4-year-old female Miniature Schnauzer was referred for necropsy. For 3 months prior to death, the dog had displayed generalized alopecia, pruritus, and severe weight loss, Initially, a private veterinarian had suspected leishmaniasis. Gross examination was performed and samples for cytopathology, histopathology, and fungal culture were obtained. The macroscopic examination revealed generalized alopecia, congested mucosa, cachexia, hyperemia of the brain, and multiple white nodules measuring 0.5 cm to 4 cm in the lungs and the kidneys. None of the other organs showed significant lesions. Cytopathological examination of the nodules revealed a highly cellular sample, with a severe chronic inflammatory response, characterized by multinucleated giant cells and round-to-elliptical, yeast-shaped structures (5-10 µm), suggestive of Cryptococcus organisms. Histopathological examination of the lungs, kidneys, and brain revealed a severe diffuse chronic inflammatory process, with lymphocytic infiltration and multinucleated giant cells; countless yeast-shaped, round-to-ovoid structures (similar in appearance to “soap bubbles”) that were negative in hematoxylin-eosin stain were also present.[...]

Criptococose sistêmica em um canino da raça Miniatura Schnauzer / Systemic cryptococcosis in a Miniature Schnauzer dog

Background: Cryptococcosis is an important zoonotic disease that occurs worldwide. The disease is caused by a soilborne opportunistic fungus of the genus Cryptococcus, which can also be found in the feces of birds, especially pigeons. In Brazil, the geographical distribution of the agent is fairly defined, with the species C. gattii predominantly found in the north-eastern states. Diagnosis is based on the clinical history, physical examination findings, and results of complementary testing, such as cytopathological and histopathological examinations. This report aims to describe the clinical and anatomopathological findings in a case of systemic cryptococcosis in a dog.Case: A 4-year-old female Miniature Schnauzer was referred for necropsy. For 3 months prior to death, the dog had displayed generalized alopecia, pruritus, and severe weight loss, Initially, a private veterinarian had suspected leishmaniasis. Gross examination was performed and samples for cytopathology, histopathology, and fungal culture were obtained. The macroscopic examination revealed generalized alopecia, congested mucosa, cachexia, hyperemia of the brain, and multiple white nodules measuring 0.5 cm to 4 cm in the lungs and the kidneys. None of the other organs showed significant lesions. Cytopathological examination of the nodules revealed a highly cellular sample, with a severe chronic inflammatory response, characterized by multinucleated giant cells and round-to-elliptical, yeast-shaped structures (5-10 µm), suggestive of Cryptococcus organisms. Histopathological examination of the lungs, kidneys, and brain revealed a severe diffuse chronic inflammatory process, with lymphocytic infiltration and multinucleated giant cells; countless yeast-shaped, round-to-ovoid structures (similar in appearance to “soap bubbles”) that were negative in hematoxylin-eosin stain were also present.[...](AU)

Eficácia “in vitro” do extrato etanólico das folhas de Hyptis suaveolens (L) Poit (Lamiaceae) sobre fêmeas ingurgitadas de Rhipicephalus (Boophilus) microplus (Canestrini, 1887) / “In vitro” efficacy of ethanolic extract from the leaves of suaveolens (L) Poit (Lamiaceae) against engorged females of Rhipicephalus (Boophilus) microplus (Canestrini, 1887)

This experiment was carried out to tudy the bioacaricidal efficacy of ethanolic extract from the leaves of Hyptis suaveolens (L) Poit (Lamiaceae) against engorged females of Rhipicephalus (Boophilus) microplus. 100 engorged females of R. (B.) microplus were manually collected from a naturally infested herd. The females were subjected to the immersion test at three concentrations of the ethanolic extract: 0,1; 1,0 and 10µg/mL, with two replications in each reatment. It was ound efficacy of 10% in all concentrations. The data found in this study show that the ethanolic extract from the leaves of H. Suaveolens do not have bioacaricidal activity at the used concentrations.

Salmonelose em potro: relato de caso / Salmonellosis in foal: a case report

This case report aimed to show a foal treatment with acute salmonellosis. The male foal was a month years old American quarter horse, weighing 51 kg. In its anamnesis, it wasreported that the animal had fetid yellowish-green diarrhea evolving faster. On clinical examination, the animal presented absent appetite, dehydration, high capillary refill time, enlarged lymphnodes, fever, a heart rate of 44 bpm, respiratory of 15 mpm and intestinal hypermotility. The diagnosis was based on clinical suspicion. In the first hours of hospitalization, it was administered drugs aiming to restore the loss of fluids and electrolytes, decreased fluid secretion, control of endotoxemia and restoration of normal microbiota. The proposed treatment was effective in controlling equine salmonellosis, recovering the physiological condition of the patient.

Salmonelose em potro: relato de caso / Salmonellosis in foal: a case report

This case report aimed to show a foal treatment with acute salmonellosis. The male foal was a month years old American quarter horse, weighing 51 kg. In its anamnesis, it wasreported that the animal had fetid yellowish-green diarrhea evolving faster. On clinical examination, the animal presented absent appetite, dehydration, high capillary refill time, enlarged lymphnodes, fever, a heart rate of 44 bpm, respiratory of 15 mpm and intestinal hypermotility. The diagnosis was based on clinical suspicion. In the first hours of hospitalization, it was administered drugs aiming to restore the loss of fluids and electrolytes, decreased fluid secretion, control of endotoxemia and restoration of normal microbiota. The proposed treatment was effective in controlling equine salmonellosis, recovering the physiological condition of the patient.(AU)

Eficácia “in vitro” do extrato etanólico das folhas de Hyptis suaveolens (L) Poit (Lamiaceae) sobre fêmeas ingurgitadas de Rhipicephalus (Boophilus) microplus (Canestrini, 1887) / “In vitro” efficacy of ethanolic extract from the leaves of suaveolens (L) Poit (Lamiaceae) against engorged females of Rhipicephalus (Boophilus) microplus (Canestrini, 1887)

This experiment was carried out to tudy the bioacaricidal efficacy of ethanolic extract from the leaves of Hyptis suaveolens (L) Poit (Lamiaceae) against engorged females of Rhipicephalus (Boophilus) microplus. 100 engorged females of R. (B.) microplus were manually collected from a naturally infested herd. The females were subjected to the immersion test at three concentrations of the ethanolic extract: 0,1; 1,0 and 10µg/mL, with two replications in each reatment. It was ound efficacy of 10% in all concentrations. The data found in this study show that the ethanolic extract from the leaves of H. Suaveolens do not have bioacaricidal activity at the used concentrations.(AU)