Trypanosoma vivax em bovino leiteiro / Trypanosoma vivax in dairy cattle

Background: Trypanosoma vivax is a protozoan that causes reproductive disorders and decreased production in domestic and wild ungulate animals. The bovine are the main hosts of the disease and the transmission occurs by the bite of hematophagous insects, mainly tabanids. Several diagnostic techniques can be used to detect the parasite, both in parasitological form and by serological kits. In Brazil, the disease has been reported in bovines, goats and sheep of some states, with high morbidity and mortality and due to the scarcity of results on the epidemiology of the disease, this work had the objective to report the presence of T. vivax in a female bovine of a dairy herd in Parnaíba county, Piauí.Case: The animal naturally infected by Trypanosoma vivax, was a three-year-old cow from a dairy farm in the Parnaíba county, located in the north of Piauí state. The farm had a herd whith 62.20% of young Girolando breed cows and the breeding system used was semi-confinement, with two mechanical milking per day. At the time of a Veterinarian’s technical visit to the property, it was observed the occurrence of abortions, mastitis, estrus repetitions and cows with hematuria, leading to the suspicion of the bovine leptospirosis occurrence. Blood samples were collected from 78 cows from the herd for hematological, biochemical and serological tests, and 72 (92.30%) were reactive to some Leptospira serovars. All the exams were carried out at the Federal University of Piauí (UFPI). In the group of animals negative for leptospirosis, a female was diagnosed positive for bovine trypanosomiasis, confirming the result in the blood smear. This animal had no clinical signs characteristic of the disease at the time of the evaluation.[...]

Trypanosoma vivax em bovino leiteiro / Trypanosoma vivax in dairy cattle

Background: Trypanosoma vivax is a protozoan that causes reproductive disorders and decreased production in domestic and wild ungulate animals. The bovine are the main hosts of the disease and the transmission occurs by the bite of hematophagous insects, mainly tabanids. Several diagnostic techniques can be used to detect the parasite, both in parasitological form and by serological kits. In Brazil, the disease has been reported in bovines, goats and sheep of some states, with high morbidity and mortality and due to the scarcity of results on the epidemiology of the disease, this work had the objective to report the presence of T. vivax in a female bovine of a dairy herd in Parnaíba county, Piauí.Case: The animal naturally infected by Trypanosoma vivax, was a three-year-old cow from a dairy farm in the Parnaíba county, located in the north of Piauí state. The farm had a herd whith 62.20% of young Girolando breed cows and the breeding system used was semi-confinement, with two mechanical milking per day. At the time of a Veterinarians technical visit to the property, it was observed the occurrence of abortions, mastitis, estrus repetitions and cows with hematuria, leading to the suspicion of the bovine leptospirosis occurrence. Blood samples were collected from 78 cows from the herd for hematological, biochemical and serological tests, and 72 (92.30%) were reactive to some Leptospira serovars. All the exams were carried out at the Federal University of Piauí (UFPI). In the group of animals negative for leptospirosis, a female was diagnosed positive for bovine trypanosomiasis, confirming the result in the blood smear. This animal had no clinical signs characteristic of the disease at the time of the evaluation.[...](AU)

Maturação de oócitos bovinos em meio suplementado com captopril / Maturation of Bovine oocytes in medium supplemented with captopril

The aim of this research was to evaluate the effects of supplementation of maturation medium withcaptopril in oocyte in vitro maturation (IVM). 470 CCOs were recovered and classified into grades I and II,being divided into four groups: G1 (n = 56), that was control group; G2 (n = 152) 20 mM of captopril; G3 (n =126) 40 uM of captopril; and G4 (n = 136) 80 uM of captopril, then these were subsequently submitted to IVMprocess for 24 hours at 38.5°C in 5% CO2. After 24 hours of maturation, oocytes were denuded and evaluatedfor the first polar body extrusion and were, therefore, considered matured. The addition of the Captopril inmedium of oocytes maturation has not improved the IVM rate.(AU)

Avaliação morfológica, funcional e tempo de termorresistência de espermatozoides caprinos localmente adaptados / Morphological, functional and thermoresistance time evaluation of locally adapted goats sperm

This study evaluated the quality of ejaculated from adapted breeds of goats as the morphologic aspects,functional and thermoresistance. An aliquot of the pool of each breed was used for morphological evaluation bywet preparation, quantifying sperm defects and dividing them into classes. The samples were cryopreservedusing TK3000 machine and evaluated by thermoresistance test by checking motility and sperm vigor every hour.For plasma membrane functionality analysis, was used hiposmotic test. There was no difference (P > 0.05)between the breeds in all sperm defects before and after cryopreservation, as well as for the thermoresistancetimes for motility and sperm vigor (P > 0.05). There was a significant reduction in motility and sperm vigor 120minutes after thawing of the samples. There was no difference (P > 0.05) between the percentage of sperm withfunctional membrane between the evaluated breeds. Therefore, the evaluated breeds have morphology,functionality and similar sperm thermoresistance.(AU)

Maturação de oócitos bovinos em meio suplementado com captopril / Maturation of Bovine oocytes in medium supplemented with captopril

The aim of this research was to evaluate the effects of supplementation of maturation medium withcaptopril in oocyte in vitro maturation (IVM). 470 CCOs were recovered and classified into grades I and II,being divided into four groups: G1 (n = 56), that was control group; G2 (n = 152) 20 mM of captopril; G3 (n =126) 40 uM of captopril; and G4 (n = 136) 80 uM of captopril, then these were subsequently submitted to IVMprocess for 24 hours at 38.5°C in 5% CO2. After 24 hours of maturation, oocytes were denuded and evaluatedfor the first polar body extrusion and were, therefore, considered matured. The addition of the Captopril inmedium of oocytes maturation has not improved the IVM rate.

Avaliação morfológica, funcional e tempo de termorresistência de espermatozoides caprinos localmente adaptados / Morphological, functional and thermoresistance time evaluation of locally adapted goat’s sperm

This study evaluated the quality of ejaculated from adapted breeds of goats as the morphologic aspects,functional and thermoresistance. An aliquot of the pool of each breed was used for morphological evaluation bywet preparation, quantifying sperm defects and dividing them into classes. The samples were cryopreservedusing TK3000 machine and evaluated by thermoresistance test by checking motility and sperm vigor every hour.For plasma membrane functionality analysis, was used hiposmotic test. There was no difference (P > 0.05)between the breeds in all sperm defects before and after cryopreservation, as well as for the thermoresistancetimes for motility and sperm vigor (P > 0.05). There was a significant reduction in motility and sperm vigor 120minutes after thawing of the samples. There was no difference (P > 0.05) between the percentage of sperm withfunctional membrane between the evaluated breeds. Therefore, the evaluated breeds have morphology,functionality and similar sperm thermoresistance.