Resumo
O objetivo deste trabalho foi verificar a presença de Brucella abortus e as lesões causadas por esse agente nos anexos fetais e nos fetos de búfalas. Para isso, 20 búfalas em diversos meses de gestação, sorologicamente positivas para brucelose, foram submetidas ao abate sanitário. A idade fetal foi determinada através de exames ultrassonográficos associados à mensuração dos fetos durante a necropsia. Do útero fechado desses animais foram coletadas amostras para histopatologia e qPCR. A partir do segundo mês de gestação foi possível detectar a presença de DNA de B. abortus em líquido amniótico, líquido alantoide e em útero e, a partir do quinto mês, na placenta, coração, baço, rim, pulmão, intestino, fígado e linfonodos dos fetos. Os principais achados anatomopatológicos foram placentite fibrinopurulenta necrótica e endometrite supurativa crônica.(AU)
The objective of this study was to detect Brucella abortus and injuries caused by the bacteria in fetal membranes and fetuses. Twenty buffaloes serologically positive for brucellosis were used and subjected to stamping for collection of material from the closed uterus of several months gestation. Fetal age was determined by ultrasound examination and the size of fetuses was measured at necropsy. The samples were subjected to histopathology and qPCR. From the second month of pregnancy on it was possible to detect the presence of B. abortus DNA in amniotic fluid, allantoic liquid and uterus, and from the fifth month on in placenta, heart, spleen, kidney, lung, intestine, liver and lymph nodes of the fetuses. The main pathological findings were fibrinous suppurative necrotic placentitis, and chronic endometritis.(AU)
Assuntos
Animais , Brucella abortus/isolamento & purificação , Lesões Pré-Natais/diagnóstico , Lesões Pré-Natais/veterinária , Búfalos/lesões , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Brucelose/veterinária , Prenhez , Doenças Placentárias/veterinária , Endometrite/veterináriaResumo
A recent (November 2010) outbreak of infectious laryngotracheitis (ILT) in a multi-age laying hen facility in Minas Gerais state, Brazil, is described. Previous ILT outbreak in laying hens was only notified in São Paulo state, Brazil, in 2002. In the outbreak described here, the affected population was approximately eight million hens, with flock sizes ranging from 100,000 to 2,900,000 chickens. The average mortality ranged from 1 to 6%, and morbidity was around 90% (most of the twenty seven farms of the area were positive for ILT virus). Three multi-age laying farms from one company were selected for this report. Clinical signs included prostration, dyspnea, conjunctivitis, occasional swelling of the paranasal sinuses and bloody mucous nasal discharge. Severely affected chickens presented with dyspnea, gasping and became cyanotic before death. At necropsy, these chickens had fibrinous exudate blocking the larynx and the lumen of cranial part of the trachea. In addition, conjunctivitis with intense hyperemia, edema and sinuses with caseous exudate were present. On histopathology, there were marked necrosis and desquamation of respiratory ephitelium and conjunctiva with numerous syncytial cells formation and fibrinous exudate. Moderate to marked non suppurative (especially lymphocytes and plasma cells) infiltration in the lamina propria also was observed. Sixteen out of 20 examined chickens, eosinophilic intranuclear inclusion bodies were observed in the syncytial cells. The DNA extracted from larynx and trachea produced positive PCR results for ILT virus (ILTV) DNA using formalin-fixed, paraffin embedded (FFPE) samples. Amplicons from a small region of ICP4 gene were submitted to sequencing and showed 100% identity with ILTV EU104910.1 (USA strain), 99% with ILTV JN596963.1 (Australian strain) and 91% with ILTV JN580316.1 (Gallid herpesvirus 1 CEO vaccine strain) and JN580315.1 (Gallid herpesvirus 1 TCO vaccine strain).(AU)
Um surto recente (Novembro de 2010) de laringotraqueite infecciosa (LTI) em granjas de postura de múltiplas idades em Minas Gerais, Brasil, é descrito. Um surto de LTI em galinhas de postura havia sido previamente relatado apenas no Estado de São Paulo em 2002. No surto aqui descrito, a população afetada foi de aproximadamente oito milhões de galinhas, com lotes variando de 100.000 a 2.900.000 galinhas. A mortalidade média variou de 1 a 6% e a morbidade atingiu cerca de 90% (a maioria das 27 granjas foram positivas para o virus da LTI). Três granjas com aves de múltiplas idades pertencentes a uma empresa foram selecionadas para o presente relato. Os sinais clinicos incluíram prostração, dispneia, conjuntivite, edema ocasional dos seios paranasais e secreção nasal mucosa e/ou sanguinolenta. As aves severamente afetadas apresentaram acentuada dispneia, aparente engasgo e tornaram-se cianóticas antes da morte. Nestas aves, exsudato fibrinoso denso obstruindo o lúmen da laringe e parte cranial da traqueia foi observado na necropsia. Havia também, conjuntivite com hiperemia intensa e edema, além de sinusite com exsudato caseoso. Na histopatologia, observaram-se necrose e descamação acentuada do epitélio respiratório e da conjuntiva com formação de numerosos sincícios e exsudato fibrinoso. Além disso, infiltrado inflamatório mononuclear (especialmente linfócitos e plasmócitos) moderado a acentuado na lâmina própria foi observado. Corpúsculos de inclusão intranucleares nas células sinciciais foram observados em 16 das 20 aves examinadas. Resultados positivos pela PCR para o virus da LTI foram obtidos de DNA extraído das laringes e traqueias utilizando amostras fixadas em formol e incluidas na parafina. O produto amplificado de uma região pequena do gen ICP4 foi submetido ao sequenciamento e quando comparado com outras sequências depositadas no Genbank mostrou os seguintes resultados: 100% de identidade com uma estirpe do virus de LTI dos Estados Unidos (JN596963.1), 99% de identidade com uma estirpe Australiana e 91% com a estirpe vacinal CEO (JN580316.1) e TCO (JN580315.1).(AU)
Assuntos
Animais , Galinhas/virologia , Herpesvirus Galináceo 1/isolamento & purificação , Reação em Cadeia da Polimerase/veterinária , Dispneia/veterináriaResumo
A twelve-day-old foal was admitted to the Veterinary School of UFMG with profuse diarrhea. The animal was treated with several medications, including antibiotics. Despite the clinical care, the clinical condition did not improve. Due to poor prognosis, the foal was euthanized. Gross and histopathological examinations, showed pseudomembranous and/or membranous glossitis, esophagitis and gastritis with intense amount of pseudohyphae and blastoconidia compatible with Candida spp Also, fibrinous and necrotizing typhlocolitis was observed. Clostridium difficile was isolated from intestinal contents which were also positive for toxins A/B by ELISA. Using a multiplex-PCR assay, genes encoding toxin A (tcdA) and toxin B (tcdB) were detected. The severe lesions caused by Candida in this foal probably occurred due to changes in the microbiota induced by the treatment with antibiotics. However, the possibility of an acquired immunodeficiency cannot be excluded because information about quantity and quality of the colostrum ingested by this foal was not obtained.(AU)
Assuntos
Animais , Candidíase/diagnóstico , Infecções por Clostridium/diagnóstico , Cavalos/microbiologia , Candida/patogenicidade , Clostridioides difficileResumo
Canine mastitis is an infrequent condition that occurs most commonly in the postpartum period due to ascending bacterial infection. This report aims to describe clinical, pathological and bacteriological aspects of mastitis caused by Staphylococcus hyicus accompanied by disseminated superficial lymphadenitis in a dog. In the postpartum period the animal showed clinical signs of prostration, dyspneia, and unresponsivess to environment. At physical examination, hypothermia, dehydration and subcutaneous edema of the limbs were detected. Gross and histologic features were consistent with marked purulent and necro-hemorrhagic mastitis accompanied by lymphangitis, lymphadenitis and thromboembolic pneumonia. Isolation and biochemical identification confirmed the infection by Staphylococcus hyicus. This study shows that one must be attentive not only of the risk of uterine infection postpartum, but also of the possibility of mammary gland infection.(AU)
Assuntos
Animais , Feminino , Cães , Infecções Estafilocócicas/diagnóstico , Hipotermia/veterinária , Linfadenite/diagnóstico , Mastite/diagnóstico , Período Pós-Parto , Staphylococcus hyicusResumo
This paper describes an outbreak of chlamydiosis psittacines recovered in Minas Gerais, Brazil, from illegal trade in the Brazilian Southeastern Region. Clinically, some birds showed apathy and anorexia or died suddenly without evidence of clinical signs. Necropsy was performed on 15 psittacines; 13 Amazon parrots (4. aestiva), one Peach-fronted parakeet (Aratinga aurea) and one Scaly-headed parrot (Pionus maximiliani). The most visible macroscopic changes were mild to marked hepatomegaly, with many white ranging in from 1 mm 100 that extended in to the parenchyma. Other findings included splenomegaly and fibrinopurulent airsacculitis. Microscopic lesions included multifocal to coalescing foci of necrosis of hepatocytes with infiltration of heterophils and lymphocytes and plasma cells randomly scattered throughout. In some parrots there were multiple foci of necrotic and granulomatous hepatitis with biliary duct proliferation. The birds with splenomegaly had loss of normal architecture and infiltration of macrophages mixed with plasma cells, fibrin and heterophils. The PVK (modified Gimenez) and Giemsa stains revealed small round intracytoplasmic bacteria approximately 1um in diameter suggestive of Chlamydophila psittaci, in the cytoplasm of macrophages of spleens and livers. Immunohistochemistry (IHC) using monoclonal antibodies for C. psitacci confirmed Chlamydophila in the cytoplasm of macrophages of the liver and spleen and in a few other organs of four parrots.