Influence of Phytase with Or without Organic Acid (Sodium Di-Formate) Supplementation on Growth Performance, Carcass Response, Protein and Mineral Digestibility in Starter Phase of Broilers

This experiment was planned to evaluate the effects of phytase supplementation with or without organic acid (OA) on performance, protein, calcium (Ca), phosphorus (P) and sodium (Na) digestibility and carcass parameters in broiler chickens fed low Ca and low P diets in comparison to a high Ca and high P diet with standard specifications. For this purpose, two iso-nitrogenous and iso-caloric diets were prepared in such a way that one diet had high Ca (9.80) and high P (4.50) and second diet had low Ca (8.50) and low P (2.40), respectively. Low Ca and low P diet was further supplemented with enzyme phytase @ 500 FTU/kg, and phytase @ 500 FTU/kg + organic acid (1 kg/ton). Rations were offered to seven replicates of ten birds each, from day 1 to 21. Results revealed that the experimental treatments had no effect on feed intake and growth performance of birds (p>0.05). Carcass parameter results showed highest thigh meat yield % and liver weight % in the birds fed diet with low Ca & P and supplemented with enzymes phytase (p<0.05). Fecal P and Na digestiblities were high (p<0.05) in experimental broilers fed low Ca low P with enzyme phytase supplementation as compared to the diet without phytase supplementation. It is concluded that phytase and OA supplementation in the starter diet with low Ca and low P level did not influence intake, growth and feed conversion of the broilers. However, thigh meat yield percent and mineral digestiblities (P and Na) increased in the broilers that received the diet with low Ca and low P level and supplemented with enzyme phytase.(AU)

Effect of Exogenous Emulsifier (Lyso-Phospholipid) Supplementation in the Broiler Diet, on the Feed Intake and Growth Performance During Grower Phase

The objective of this experiment was to investigate the effect of supplementation of an exogenous emulsifier (lyso-phospholipid) in the diet of growing broilers on growth performance and digestibility. A total of 1224 Ross-308 ten day old broiler chicks were distributed into two experimental treatments in such a way that each treatment had twelve replicates with fifty-one birds per replicate. Two experimental diets were formulated with and without emulsifier supplementation according to the nutrition standards of Ross 308. Feed intake and body weight gain of the broilers were measured on a daily basis and feed conversion ratio was also calculated. Nutrient digestibility was determined on the 25th day of age. Analysis of variance under completely randomized design technique was used to analyze the data. Feed intake was increased (p 0.05) by supplementation of emulsifier in the broiler diet on theat 12th, 13th, 21th, 22th, 23th, 24th, and 25th days. Bodyweight gain was not affected (p>0.05) with or without emulsifier supplementation in the broiler diet during 11-25 days of life. However, feed conversion ratio was effected (p 0.05) by emulsifier supplementation and increased from days 21-25th. Nutrient digestibility (dry matter, fat, and crude protein) in the grower phase was decreased (p 0.05) by supplementation of emulsifier in the diet. It can be concluded that supplementation of an exogenous emulsifier in the diet did not show positive effect on the growth performance during the grower phase of broilers, while nutrient digestibility showed adverse effect. Emulsifier supplementation should be tested after 25 days of the life of broilers.(AU)

Effect of Exogenous Emulsifier (Lyso-Phospholipid) Supplementation in the Broiler Diet, on the Feed Intake and Growth Performance During Grower Phase

The objective of this experiment was to investigate the effect of supplementation of an exogenous emulsifier (lyso-phospholipid) in the diet of growing broilers on growth performance and digestibility. A total of 1224 Ross-308 ten day old broiler chicks were distributed into two experimental treatments in such a way that each treatment had twelve replicates with fifty-one birds per replicate. Two experimental diets were formulated with and without emulsifier supplementation according to the nutrition standards of Ross 308. Feed intake and body weight gain of the broilers were measured on a daily basis and feed conversion ratio was also calculated. Nutrient digestibility was determined on the 25th day of age. Analysis of variance under completely randomized design technique was used to analyze the data. Feed intake was increased (p 0.05) by supplementation of emulsifier in the broiler diet on theat 12th, 13th, 21th, 22th, 23th, 24th, and 25th days. Bodyweight gain was not affected (p>0.05) with or without emulsifier supplementation in the broiler diet during 11-25 days of life. However, feed conversion ratio was effected (p 0.05) by emulsifier supplementation and increased from days 21-25th. Nutrient digestibility (dry matter, fat, and crude protein) in the grower phase was decreased (p 0.05) by supplementation of emulsifier in the diet. It can be concluded that supplementation of an exogenous emulsifier in the diet did not show positive effect on the growth performance during the grower phase of broilers, while nutrient digestibility showed adverse effect. Emulsifier supplementation should be tested after 25 days of the life of broilers.

Effects of Bile Acids and Lipase Supplementation in Low-Energy Diets on Growth Performance, Fat Digestibility and Meat Quality in Broiler Chickens

The aim of this study was to investigate the effect of bile acids and lipase supplementation in low energy (LE) diets on growth, fat digestibility, serum lipid profile and meat quality of broilers. Seven hundred one-day-old broiler chicks were divided into 5 dietary treatments with five replicates of 28 birds each. The five treatments were: i) high energy diet (HE; metabolizable energy (ME) = 3,000 and 3,170 kcal/kg for starter and finisher diet), ii) low energy diet (LE; ME = 2,900 and 3,070 kcal/kg for starter and finisher diet), iii) LE diet supplemented with 300 g/ton bile acids (LEB), iv) LE diet supplemented with 180 g/ton lipase (LEL), v) LE diet supplemented both with bile acids (300 g/ton) and lipase (180 g/ton). The experiment lasted 35 days having starter phase from days 1-21 and finisher phase from days 22-35. Dietary inclusion of both bile acids and lipase in LE diet had no effect (p>0.05) on body weight (BW) gain and feed intake. High energy diet reduced feed intake and BW gain during starter and overall period; however, during finisher phase BW gain was similar in all dietary treatments. Dietary energy level had no effect on feed conversion ratio. Fat digestibility (p>0.05) both in the starter and finisher phase was not affected by the dietary treatments. Concentration of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and triglycerides were not affected by the dietary treatments (p>0.05). Meat quality of breast and thigh muscle was unaffected due to the dietary treatments (p>0.05). It is concluded that the supplementation of bile acids alone or in combination with lipase in low-energy diets did not improve broiler performance, fat digestibility, serum lipid profile and meat quality.(AU)

Emulsifier Supplementation Response in Ross 308 Broilers at 1-10 Days

The study was conducted to check the effect of emulsifier supplementation on growth performance of broiler birds on starter phase, from days 1 to 10. In the current study, a total of twelve hundred twenty-four Ross 308 broiler one day old chicks were used. Ross 308 broiler chicks were divided into two groups in such a way that each group had12 replicates, and each replicate had 51 birds. The duration of the current trial was of 10 days. Chicks were fed experimental diets with or without emulsifier supplementation. Daily feed intake, body weight, feed conversion ratio and nutrient digestibility were recorded. Mortality was recorded on a daily basis, and feed intake was also corrected for mortality. Data collected from the experiment was interpreted by using analysis of variance method using SPSSTM under Completely Randomized Design. Results showed that emulsifier supplementation improved (p 0.05) feed intake in broiler birds on the 2nd, 6th, 7th and 9th­ days of the starter phase. Furthermore, emulsifier supplementation (p 0.05) affected the body weight on the 3rd and 10th day of the trial. Emulsifier supplementation also affected (p 0.05) feed conversion ratio on the 10th day of the trial. However, nutrient digestibility was not affected by the supplementation of emulsifier in the diet of broiler chicks. Results showed that emulsifier supplementation should be started on day 10.(AU)

Effects of Bile Acids and Lipase Supplementation in Low-Energy Diets on Growth Performance, Fat Digestibility and Meat Quality in Broiler Chickens

The aim of this study was to investigate the effect of bile acids and lipase supplementation in low energy (LE) diets on growth, fat digestibility, serum lipid profile and meat quality of broilers. Seven hundred one-day-old broiler chicks were divided into 5 dietary treatments with five replicates of 28 birds each. The five treatments were: i) high energy diet (HE; metabolizable energy (ME) = 3,000 and 3,170 kcal/kg for starter and finisher diet), ii) low energy diet (LE; ME = 2,900 and 3,070 kcal/kg for starter and finisher diet), iii) LE diet supplemented with 300 g/ton bile acids (LEB), iv) LE diet supplemented with 180 g/ton lipase (LEL), v) LE diet supplemented both with bile acids (300 g/ton) and lipase (180 g/ton). The experiment lasted 35 days having starter phase from days 1-21 and finisher phase from days 22-35. Dietary inclusion of both bile acids and lipase in LE diet had no effect (p>0.05) on body weight (BW) gain and feed intake. High energy diet reduced feed intake and BW gain during starter and overall period; however, during finisher phase BW gain was similar in all dietary treatments. Dietary energy level had no effect on feed conversion ratio. Fat digestibility (p>0.05) both in the starter and finisher phase was not affected by the dietary treatments. Concentration of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and triglycerides were not affected by the dietary treatments (p>0.05). Meat quality of breast and thigh muscle was unaffected due to the dietary treatments (p>0.05). It is concluded that the supplementation of bile acids alone or in combination with lipase in low-energy diets did not improve broiler performance, fat digestibility, serum lipid profile and meat quality.

Emulsifier Supplementation Response in Ross 308 Broilers at 1-10 Days

The study was conducted to check the effect of emulsifier supplementation on growth performance of broiler birds on starter phase, from days 1 to 10. In the current study, a total of twelve hundred twenty-four Ross 308 broiler one day old chicks were used. Ross 308 broiler chicks were divided into two groups in such a way that each group had12 replicates, and each replicate had 51 birds. The duration of the current trial was of 10 days. Chicks were fed experimental diets with or without emulsifier supplementation. Daily feed intake, body weight, feed conversion ratio and nutrient digestibility were recorded. Mortality was recorded on a daily basis, and feed intake was also corrected for mortality. Data collected from the experiment was interpreted by using analysis of variance method using SPSSTM under Completely Randomized Design. Results showed that emulsifier supplementation improved (p 0.05) feed intake in broiler birds on the 2nd, 6th, 7th and 9th­ days of the starter phase. Furthermore, emulsifier supplementation (p 0.05) affected the body weight on the 3rd and 10th day of the trial. Emulsifier supplementation also affected (p 0.05) feed conversion ratio on the 10th day of the trial. However, nutrient digestibility was not affected by the supplementation of emulsifier in the diet of broiler chicks. Results showed that emulsifier supplementation should be started on day 10.

Effect of Fat Sources and Emulsifier Supplementation in Broiler Starter, Grower and Finisher Diets on Performance, Nutrient Digestibility, and Carcass Parameters

The purpose of this study was to evaluate the effect of fat sources and emulsifier supplementation in different phases on growth performance of broilers. Treatments were; (T1) basal ration (BR) which contained soy oil (SO) and had inclusion of emulsifier for whole life, (T2) BR which contained poultry fat (PO) and had inclusion of emulsifier for whole life, (T3) BR which contained oxidized oil (OO) and had inclusion of emulsifier for whole life, (T4) BR which contained SO and had inclusion of emulsifier during starter phase (T5) BR which contained PO and had inclusion of emulsifier during the starter phase, (T6) BR which contained OO and had inclusion of emulsifier during the starter phase, (T7) BR which contained SO and had inclusion of emulsifier during the starter phase (T8) BR which contained PO and had inclusion of emulsifier during the grower phase, (T9) BR which contained OO and had inclusion of emulsifier during the grower phase, (T10) BR which contained SO and had inclusion of emulsifier during the finisher phase, (T11) BR which contained PO and had inclusion of emulsifier during the finisher phase (T12) BR which contained OO and had inclusion of emulsifier during the finisher phase. The Basal ration which contained SO and supplemented emulsifier during the grower phase and throughout life increased the body weight gain. The supplementation of emulsifier in the finisher phase and throughout life in a diet which contained SO had better feed conversion ratio. The supplementation of emulsifier in the finisher phase and throughout life in SO based diet increased the dry matter and crude fat digestibility. We recommend emulsifier supplementation in the finisher phase for the economic point of view.(AU)

Effect of Fat Sources and Emulsifier Supplementation in Broiler Starter, Grower and Finisher Diets on Performance, Nutrient Digestibility, and Carcass Parameters

The purpose of this study was to evaluate the effect of fat sources and emulsifier supplementation in different phases on growth performance of broilers. Treatments were; (T1) basal ration (BR) which contained soy oil (SO) and had inclusion of emulsifier for whole life, (T2) BR which contained poultry fat (PO) and had inclusion of emulsifier for whole life, (T3) BR which contained oxidized oil (OO) and had inclusion of emulsifier for whole life, (T4) BR which contained SO and had inclusion of emulsifier during starter phase (T5) BR which contained PO and had inclusion of emulsifier during the starter phase, (T6) BR which contained OO and had inclusion of emulsifier during the starter phase, (T7) BR which contained SO and had inclusion of emulsifier during the starter phase (T8) BR which contained PO and had inclusion of emulsifier during the grower phase, (T9) BR which contained OO and had inclusion of emulsifier during the grower phase, (T10) BR which contained SO and had inclusion of emulsifier during the finisher phase, (T11) BR which contained PO and had inclusion of emulsifier during the finisher phase (T12) BR which contained OO and had inclusion of emulsifier during the finisher phase. The Basal ration which contained SO and supplemented emulsifier during the grower phase and throughout life increased the body weight gain. The supplementation of emulsifier in the finisher phase and throughout life in a diet which contained SO had better feed conversion ratio. The supplementation of emulsifier in the finisher phase and throughout life in SO based diet increased the dry matter and crude fat digestibility. We recommend emulsifier supplementation in the finisher phase for the economic point of view.

Size at sexual maturity estimation for 36 species captured by bottom and mid-water trawls from the marine habitat of Balochistan and Sindh in the Arabian Sea, Pakistan, using maximum length (Lmax) and logistic (L50) models / Estimativa do tamanho na maturidade sexual de 36 espécies capturadas por redes de arrasto de fundo no hábitat marinho do Baluchistão e Sindh, no Mar Arábico, Paquistão, usando modelos de comprimento máximo (Lmax) e logístico (L50)

The aim of this study was to estimate the size at first sexual maturity (Lm) for 36 species belonging to 24 families from the marine habitat of Balochistan and Sindh in Arabian Sea, Pakistan through maximum length based an empirical and logistic model using commercial catch during June 2020 to May 2021. Individual total length (TL) was noted up to 0.1 cm using measuring broad. The Lm was calculated using two formulae; (i) log (Lm) = - 0.1246 + 0.9924 * log (Lmax) for Elasmobranchs and (ii) log (Lm) = -0.1189 + 0.9157* log (Lmax) for ray-finned fishes. The minimum Lm was recorded as 10.27 cm TL for Caranx malabaricus and 108.38 cm TL for Isurus oxyrinchus, respectively. Around Lm with 58.33% species were ranges from 19.00 cm to 25.00 cm TL. This study was estimated 16 newly Lm which is globally absent and rest 20 Lm are absent in the Arabian Sea (Pakistan coastal habitats). Therefore, the results will be helpful for the sustainable management and conservation of these marine fishes through the establishment of mesh size of trawl nets based on the size at sexual maturity (Lm).

O objetivo deste estudo foi estimar o tamanho na primeira maturidade sexual (Lm) para 36 espécies pertencentes a 24 famílias do hábitat marinho do Baluchistão e Sindh, no Mar Arábico, Paquistão, por meio de modelo empírico e logístico com base no comprimento máximo usando captura comercial, durante junho de 2020 e maio de 2021. O comprimento total individual (TL) foi anotado até 0,1 cm usando medição ampla. O Lm foi calculado por duas fórmulas; (i) log (Lm) = - 0,1246 + 0,9924 * log (Lmax) para elasmobrânquios; e (ii) log (Lm) = - 0,1189 + 0,9157* log (Lmax) para peixes com nadadeiras raiadas. O Lm mínimo foi de 10,27 cm de TL para Caranx malabaricus, e o máximo, de 108,38 cm de TL para Isurus oxyrinchus. Em 58,33% das espécies com Lm foram encontradas faixas de 19,00 cm a 25,00 cm de TL. Este estudo estimou que 16 espécies com Lm estão globalmente ausentes e as 20 espécies restantes com Lm estão ausentes no Mar Arábico (hábitats costeiros do Paquistão). Portanto, os resultados serão úteis para o manejo sustentável e a conservação desses peixes marinhos por meio do estabelecimento da malhagem das redes de arrasto com base no tamanho na primeira maturidade sexual (Lm).