Adenoma of the gallbladder in a mongoose (Mungos mungo) / Adenoma de vesícula biliar em mangusto (Mungos mungo)

Primary gallbladder neoplasms occur infrequently in domestic animals and rarely in wild animals. This article describes a case of gallbladder adenoma in a mongoose ( Mungos mungo ) in captivity. Grossly, the gallbladder was moderately distended with a smooth capsular surface. Necropsy showed an irregular, soft, whitish nodule 0.8cm in diameter. Microscopic examination of the nodule showed proliferation of epithelial cells, which projected from the mucosa and formed papillary projections supported by a sparse fibrovascular stroma. Neoplastic cells were well differentiated with mild anisocytosis and anisokaryosis. Based on these histopathological findings, adenoma of the gallbladder was diagnosed.(AU)

Neoplasmas primárias de vesícula biliar são incomumente descritas em animais domésticos e, raramente, afetam animais silvestres. Este trabalho tem por objetivo descrever um caso de adenoma de vesícula biliar em mangusto ( Mungos mungo ) mantido em cativeiro. Macroscopicamente, a vesícula biliar estava moderadamente distendida, com superfície lisa e, em seu interior, havia um nódulo esbranquiçado, irregular, macio, de 0,8cm de diâmetro. Microscopicamente, havia proliferação neoplásica, epitelial, bem diferenciada, com projeções papilares da mucosa contendo células cilíndricas. Observou-se anisocitose e anisocariose leve. Com base nos achados histopatológicos, foi firmado o diagnóstico de adenoma de vesícula biliar.(AU)

Experimental infection of horses with Vaccinia virus / Infecção experimental de equinos com o vírus Vaccínia

Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) was detected. Considering the rare occurrence of VACV infection in horses, the objective of this study was to investigate the susceptibility and pathogenesis of VACV infection in this species. Six adult horses were inoculated with VACV P1V or P2V (106.3TCID50/ml) through scarification of the nasolabial surface and monitored for virological and clinical aspects during 28 days. Four inoculated horses (4/6) developed mild lesions in the site of inoculation. Ulcers and scabs restricted to inoculated areas were observed between days 2 and 8 post-inoculation (pi). Microscopically there were acanthosis, ballooning degeneration of the stratum spinosum, necrosis and loss of the epidermis. Infiltration of neutrophils, macrophages and lymphocytes were observed in the dermis. Intracytoplasmic eosinophilic inclusions were infrequently observed in degenerate keratinocytes from adjacent necrotic areas. Virus shedding was detected between days 4 and 8 pi by PCR and virus isolation (infectious virus) from the lesions of one horse inoculated with P2V. No neutralizing antibodies were detected in inoculated animals at day 28 pi. In summary, inoculation of horses with VACV P1V and P2V isolates resulted in a low level of replication and at low frequency, with mild cutaneous lesions, when compared with the course of infection of other susceptible species to VACV. Therefore, horses possibly have a low potential for viral maintenance and transmission to other species, albeit being susceptible to VACV infection.(AU)

O vírus Vaccinia (VACV) é o agente etiológico da vaccínia bovina, uma doença zoonótica re-emergente e de importância sanitária e econômica para rebanhos leiteiros. O VACV também pode ocasionalmente infectar outras espécies, incluindo equinos. Nesse sentido, um surto de VACV em equinos foi descrito em Pelotas, RS, em 2008, no qual uma coinfecção com dois isolados de VACV (denominados Pelotas 1 [P1V] e Pelotas 2 [P2V]) foi detectada. Considerando a rara ocorrência da infecção pelo VACV em equinos, o objetivo deste estudo foi investigar a susceptibilidade e a patogenia experimental da infecção pelo VACV nessa espécie. Para isso, seis equinos adultos foram inoculados com P1V ou P2V (106,3DICC50/ml) após escarificação na superfície nasolabial e monitorados para os aspectos virológicos, clínicos e patológicos por 28 dias. Quatro animais inoculados (4/6) desenvolveram lesões macroscópicas leves nos locais da inoculação, consistentes com aquelas causadas por VACV, caracterizadas por úlceras e crostas focais restritas à área inoculada, entre os dias 2 e 8 pós-inoculação (pi). Microscopicamente, havia acantose, degeneração hidrópica do estrato espinhoso e necrose da epiderme, com bactérias intralesionais. Infiltração moderada de neutrófilos, macrófagos e linfócitos foi observada na derme superficial. Inclusões intracitoplasmáticas eosinofílicas foram infrequentemente observadas nos queratinócitos degenerados adjacentes às áreas necróticas. Excreção viral foi detectada por PCR e isolamento viral das lesões de um equino inoculado com o P2V, entre os dias 4 e 8pi. Não foi detectada produção de anticorpos específicos para o VACV em nenhum animal inoculado. Em resumo, a inoculação de cavalos com os isolados P1V e P2V resultou em um baixo nível de replicação viral e em baixa frequência, resultando em lesões cutâneas mais discretas em comparação com outras espécies susceptíveis ao VACV. Apesar dos equinos serem susceptíveis à infecção pelo VACV, possivelmente apresentam baixo potencial de manutenção e de transmissão do vírus para outras espécies.(AU)

Cutaneous myxoma in a pintagol (Sporagra magellanica X Serinus canaria) / Mixoma cutâneo em um pintagol

Myxomas are benign mesenchymal tumors rarely described in birds. This report describes the clinical and pathological findings in a case of myxoma in a pintagol (Sporagra magellanicaX Serinus canaria). The animal had a nodule on the dorsal region of the third digit on the left hindlimb. Grossly, it was a 0.9×0.5×0.4cm, soft, white nodule, with black and yellow areas on the cut surface. Microscopically, a well-differentiated monomorphic population of spindle cells arranged in an abundant Alcian blue-positive myxoid matrix was observed. The diagnosis of myxoma was based on the microscopic findings.(AU)

Mixomas são tumores mesenquimais benignos incomuns em aves. Este trabalho objetiva descrever os achados clínico-patológicos de um caso de mixoma em um pintagol. A ave apresentou aumento de volume na região dorsal do terceiro dígito do membro pélvico esquerdo. Macroscopicamente, notou-se um nódulo de 0,9x0,5x0,4cm, macio, esbranquiçado, com áreas amareladas e enegrecidas na superfície de corte. A histopatologia revelou população monomórfica de células fusiformes, com baixo pleomorfismo, arranjadas em meio à matriz mixóide positiva para a coloração de azul alciano. Com base nos achados histopatológicos, foi firmado o diagnóstico de mixoma.(AU)

Eastern equine encephalitis on Federal District of Brazil and surrounding areas / Encefalomielite equina do leste no Distrito Federal e entorno

Background: Eastern equine encephalitis (EEE) is a highly lethal zoonotic disease caused by Eastern equine encephalitisvirus (EEEv), an RNA virus of the genus Alphavirus, family Togaviridae. The transmission of these alphaviruses is throughmosquitoes, mainly species of Culex, Aedes, Anopheles and Culiseta. Horses and humans are considered accidental hostsand the main reservoirs are birds and some wild rodents. The disease has been identified in Brazil by serological studies,but investigations with clinico-pathological descriptions are scarce. The present study aimed to describe the epidemiologyand clinical-pathological findings of four cases of EEE in horses from Midwestern Brazil.Materials, Methods & Results: Four confirmed cases of EEE in horses from the necropsy and histopathology files of theLaboratory of Veterinary Pathology (LPV) of the University of Brasília (UnB) were reviewed. Cases occurred betweenJanuary 2005 and April 2012. Samples of brain and spinal cord samples were fixed in formalin 10%, processed routinelyfor histopathology, and stained by hematoxylin and eosin (HE). Immuno-histochemistry (IHC) with the peroxidase streptoavidin-biotin method was done to confirm the diagnosis of EEEv infection in all cases. The slides were incubated withthe anti-EEEv monoclonal primary antibody (overnight, 1:100 dilution). The disease affected both young and adult horses.One case occurred in the summer and the other three in the fall. Clinical signs more frequently observed included circling,blindness, paresis, paralysis, somnolence, ataxia, head pressing, and recumbence...(AU)

Linfoma intravascular de células T em um cão / Intravascular t-cell lymphoma in dog / Linfoma intravascular de células t en perro

Linfoma intravascular (LI) é uma desordem linfoproliferativa rara caracterizada por proliferação intravascular de linfócitos neoplásicos com nenhum ou mínimo envolvimento do parênquima. O sistema nervoso central e a pele são os tecidos mais comumente afetados. O LI de células B é mais frequentemente relatado em comparação com os de origem T. Este trabalho descreve os achados anatomopatológicos e imuno-histoquímicos de um cão com linfoma intravascular de células T em múltiplos órgãos, incluindo encéfalo, fígado, adrenais, rins e estômago.(AU)

Intravascular lymphoma (IL) is a rare lymphoproliferative disorder characterized by intravascular proliferation of neoplastic lymphoid cells with minimal or no involvement of organs parenchyma. The central nervous system and skin are the most commonly affected tissues. B-cell IL is more frequently reported when compared with T-cell IL. This report describes the immunohistochemistry and anatomic pathology findings of a dog with intravascular T-cell lymphoma in multiple organs, including brain, liver, adrenal glands, kidneys, and stomach.(AU)

El Linfoma Intravascular (LI) es un desorden linfoproliferativo raro caracterizado por la proliferación intravascular de linfocitos neoplásicos con ninguna o mínima afectación del parénquima. El sistema nervioso central y la piel son los tejidos más comúnmente afectados. Los LI de células B han sido reportados más frecuentemente que los de origen T. Este trabajo describe los hallazgos anatomopatológicos e inmuno-histoquímicos de un perro con LI de células T en múltiples órganos, incluyendo encéfalo, hígado, adrenales, riñones y estómago.(AU)

Eastern equine encephalitis on Federal District of Brazil and surrounding areas / Encefalomielite equina do leste no Distrito Federal e entorno

Background: Eastern equine encephalitis (EEE) is a highly lethal zoonotic disease caused by Eastern equine encephalitisvirus (EEEv), an RNA virus of the genus Alphavirus, family Togaviridae. The transmission of these alphaviruses is throughmosquitoes, mainly species of Culex, Aedes, Anopheles and Culiseta. Horses and humans are considered accidental hostsand the main reservoirs are birds and some wild rodents. The disease has been identified in Brazil by serological studies,but investigations with clinico-pathological descriptions are scarce. The present study aimed to describe the epidemiologyand clinical-pathological findings of four cases of EEE in horses from Midwestern Brazil.Materials, Methods & Results: Four confirmed cases of EEE in horses from the necropsy and histopathology files of theLaboratory of Veterinary Pathology (LPV) of the University of Brasília (UnB) were reviewed. Cases occurred betweenJanuary 2005 and April 2012. Samples of brain and spinal cord samples were fixed in formalin 10%, processed routinelyfor histopathology, and stained by hematoxylin and eosin (HE). Immuno-histochemistry (IHC) with the peroxidase streptoavidin-biotin method was done to confirm the diagnosis of EEEv infection in all cases. The slides were incubated withthe anti-EEEv monoclonal primary antibody (overnight, 1:100 dilution). The disease affected both young and adult horses.One case occurred in the summer and the other three in the fall. Clinical signs more frequently observed included circling,blindness, paresis, paralysis, somnolence, ataxia, head pressing, and recumbence...

Pyogranulomatous dermatitis and panniculitis due to Nocardia nova in a cat / Dermatite e paniculite piogranulomatosas causadas por Nocardia nova em um gato

Este relato descreve os achados clínicos, patológicos e microbiológicos de uma infecção incomum por Nocardia nova em um gato. Um gato macho, sem raça definida, de 3 anos de idade, apresentou ferida cutânea exsudativa e ulcerada. Amostras da lesão foram coletadas para histopatologia e bacteriologia. Histologicamente, a lesão consistiu de dermatite e paniculite piogranulomatosas associadas a colônias grandes e irregulares de bactérias filamentosas e ramificadas. O cultivo bacteriológico revelou bacilos filamentosos, gram-positivos, parcialmente ácido resistentes, visualizados pelas colorações de Gram e Kinyoun, respectivamente. A identificação da Nocardia novafoi confirmada pelo sequenciamento 16S rDNA e análise filogenética. Este é o primeiro caso de paniculite e dermatite piogranulomatosas em um gato causado por Nocardia nova registrado no Brasil.(AU)

This report describes the clinical, pathological and microbiological findings of an uncommon infection in a cat by Nocardia nova. A 3-year-old male domestic short hair cat with an ulcerated and exudative cutaneous wound was presented for clinical examination. Samples were collected for histopathology and bacteriology diagnosis. Microscopically, the lesion was diagnosed as pyogranulomatous dermatitis and panniculitis with large and irregular colonies of branching filamentous bacterium. Skin bacteriological culture showed gram-positive rods and partially acid-fast branching filaments by gram and kinyoun staining, respectively. The identity of Nocardia nova was confirmed by 16S rDNA sequencing and phylogenetic analysis. This is the first case of pyogranulomatous dermatitis and panniculitis in a cat caused byNocardia nova reported in Brazil.(AU)

Arrhythmogenic Right Ventricular Cardiomyopathy in Two Dogs

Arrhythmogenic right ventricle cardiomyopathy (ARVC) is a rare primary heart condition recognized in dogs. It is an inherited disease more commonly described in Boxer dogs. Clinically, ARVC is characterized by ventricular tachyarrhythmias and histopathologically by fibrofatty replacement, mostly on the right ventricle. This report describes the gross and histopathological findings from a female adult Boxer and from a male adult mixed breed dog, presenting signs of heart failure and marked infiltration of adipose tissue replacing cardiomyocytes, predominantly in the epicardium and myocardium of the right ventricle.

Arrhythmogenic Right Ventricular Cardiomyopathy in Two Dogs

Arrhythmogenic right ventricle cardiomyopathy (ARVC) is a rare primary heart condition recognized in dogs. It is an inherited disease more commonly described in Boxer dogs. Clinically, ARVC is characterized by ventricular tachyarrhythmias and histopathologically by fibrofatty replacement, mostly on the right ventricle. This report describes the gross and histopathological findings from a female adult Boxer and from a male adult mixed breed dog, presenting signs of heart failure and marked infiltration of adipose tissue replacing cardiomyocytes, predominantly in the epicardium and myocardium of the right ventricle.(AU)

Plantas tóxicas para ruminantes do Sudoeste de Goiás / Toxic plants for ruminants in Southwestern Goiás, Brazil

Com o objetivo de verificar quais são as plantas incriminadas como tóxicas para ruminantes do Sudoeste de Goiás, foram realizadas 108 entrevistas com produtores rurais, médicos veterinários, zootecnistas e agrônomos de 18 municípios da região. Foram apontadas como tóxicas para ruminantes: Brachiaria spp., Enterolobium contortisiliquum, Dimorphandra mollis, Palicourea marcgravii, Pteridium aquilinum e Sorghum vulgare. Adicionalmente, foram informadas intoxicações menos frequentes por Senna occidentalis, Stryphnodendrum obovatum e Manihot esculenta. Casos isolados de intoxicação em bovinos por Asclepias curassavica e Pterodon emarginatus foram descritos por alguns entrevistados. Este trabalho demonstra que intoxicações por plantas tóxicas são frequentes na região avaliada e representam importante causa de prejuízos econômicos aos pecuaristas locais.(AU)

The aim of this study was to investigate the toxic plants for ruminants in the Southwestern region of Goiás State, Brazil. 108 people (farmers, veterinarians, zootecnists and agronomists) from 18 counties were interviewed. The following plants were described as toxic for ruminants: Brachiaria spp., Enterolobium contortisiliquum, Dimorphandra mollis, Palicourea marcgravii, Pteridium aquilinum and Sorghum vulgare. In addition, less frequent poisonings by Senna occidentalis, Stryphnodendrum obovatum e Manihot esculenta were reported. Isolated cases of poisoning by Asclepias curassavica and Pterodon emarginatus were described by some interviewers. Based in the results of this research, it is concluded that poisoning by toxic plants are frequent in the studied region and represent important cause of economic losses to the local farmers.(AU)

Diazinon acute toxicosis in cattle / Intoxicação aguda por diazinon em bovinos

The present paper reports muscarinic and nicotinic signs and death of 11 out of 23 adult cattle treated with a pour on diazinon based product, at 5-6 times the recommended concentration by the manufacturer. The diagnosis was performed by the detection of the active principle in liver and brain of cattle.(AU)

O presente trabalho descreve sinais muscarínicos, nicotínicos e morte em 11 de 23 bovinos adultos pulverizados com diazinon em concentração 5-6 vezes maior que a recomendada pelo fabricante. O diagnóstico foi confirmado pela detecção do princípio ativo no fígado e no cérebro de um bovino.(AU)

Linfoma intravascular de células t em cão

Linfoma intravascular (LI) é uma desordem linfoproliferativa rara caracterizada por proliferação intravascular de linfócitos neoplásicos com nenhum ou mínimo envolvimento do parênquima. O sistema nervoso central e a pele são os tecidos mais comumente afetados. O LI de células B é mais frequentemente relatado em comparação com os de origem T. Este trabalho descreve os achados anatomopatológicos e imuno-histoquímicos de um cão com linfoma intravascular de células T em múltiplos órgãos, incluindo encéfalo, fígado, adrenais, rins e estômago.