Resumo
Seminal plasma is a fluid with essential role in sperm functions in vivo, from ejaculation to fertilization.Among the hormones present in this medium, insulin stands out, due to its key role in the structure and motility,favoring fertilization. Insulin acts as preservation factor not able sperm and may be the crux of spermpreservation when using cryogenic processes. This study aimed to evaluate the metabolic and structuralcondition of bovine sperm cells after freezing using extenders plus different types of insulin and different types ofegg yolk. Our results showed that sperm motility was not affected by treatments used (insulin and yolk). Inconclusion, the type of egg yolk, the paw (Anas platyrhynchos), increased the viability of cryopreserved spermcells, regardless of the type of insulin used.(AU)
Assuntos
Animais , Bovinos , Células-Tronco Germinativas Adultas/classificação , Células-Tronco Germinativas Adultas/metabolismo , Células-Tronco Germinativas Adultas/fisiologia , Criopreservação , Insulina , Motilidade dos EspermatozoidesResumo
Seminal plasma is a fluid with essential role in sperm functions in vivo, from ejaculation to fertilization.Among the hormones present in this medium, insulin stands out, due to its key role in the structure and motility,favoring fertilization. Insulin acts as preservation factor not able sperm and may be the crux of spermpreservation when using cryogenic processes. This study aimed to evaluate the metabolic and structuralcondition of bovine sperm cells after freezing using extenders plus different types of insulin and different types ofegg yolk. Our results showed that sperm motility was not affected by treatments used (insulin and yolk). Inconclusion, the type of egg yolk, the paw (Anas platyrhynchos), increased the viability of cryopreserved spermcells, regardless of the type of insulin used.