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1.
Vet. Zoot. ; 23(3): 465-475, set. 2016. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: vti-686483

Resumo

Las serpientes del género Crotalus ganaron espacio significativo en el campo científico desde el desarrollo de investigaciones con su veneno que involucran la actividad citotóxica. Crotalus durissus cascavella es la única subespecie grabadas del bioma de la Caatinga del nordeste de Brasil y los estudios carecen en sus propiedades del veneno. El presente estudio evaluó el veneno crudo de C. d. cascavella por su actividad citotóxica in vitro contra líneas celulares tumorales y las células normales. El mecanismo de la muerte celular inducida por este veneno también se investigó. C. d. cascavella mostró una alta citotoxicidad (valores de IC50 que oscila desde 2,7 hasta 6,9 g/mL) contra cinco líneas de células tumorales, entre las cuales: OVCAR - 8 y SKOV3 (carcinoma de ovario), PC-3M (carcinoma metastásico de próstata), MCF-7 (carcinoma de mama), SF - 268 (glioblastoma) y PBMC también. Las células tratadas con C. d. cascavella disparó la cascada apoptótica, según lo confirmado por diversos métodos que se muestran en este trabajo. Estos resultados refuerzan así el potencial biomédico y farmacológico de las toxinas del veneno de esta especie.(AU)


Snakes from the genus Crotalus gained significant space in the scientific field since the development of researches with their venom involving cytotoxic activity. Crotalus durissus cascavella is the only subspecies recorded of the Caatinga biome of Northeastern Brazil and its noticed a lack of studies on its venom properties. The present study evaluated the crude venom of C. d. cascavella for its cytotoxic activity in vitro against both tumor cell lines and normal cells. The mechanism of cell death induced by this venom was also investigated. C. d. cascavella venom presented high cytotoxicity (IC50 values ranging from 2.7 to 6.9 µg/mL) against five tumor cells lines: OVCAR-8 and SKOV3 (ovarian carcinomas), PC-3M (metastatic prostate carcinoma), MCF-7 (breast carcinoma), and SF-268 (glioblastoma) and PBMC as well. The cells treated with C. d. cascavella venom triggered the apoptotic pathway as confirmed by several methods. These results underline the biomedical potential of toxins from the venom from this species.(AU)


Serpentes do Gênero Crotalus ganhou espaço significativo no campo científico, desde o desenvolvimento de pesquisas com seu veneno envolvendo atividade citotóxica. A Crotalus durissus cascavella (C.d.cascavella) é uma subespécie característica da Caatinga do Nordeste do Brasil e faltam estudos sobre as propriedades de seu veneno. O presente estudo avaliou o veneno bruto de C. d. cascavella para a sua atividade citotóxica in vitro contra as linhagens de células tumorais e células normais. O mecanismo de morte celular induzida por este veneno também foi investigado. Observou-se alta citotoxicidade (valores de IC50 entre 2,7-6,9 µg/mL) contra cinco linhagens de células tumorais, dentre elas: OVCAR - 8 e SKOV3 (carcinoma ovariano), PC-3M (carcinoma metastático de próstata), MCF-7 (carcinoma mamário), SF - 268 (glioblastoma) e PBMC. As células tratadas dispararam a cascata apoptótica, como confirmado por vários métodos demonstrados neste trabalho. Esses resultados reforçam, assim, o potencial biomédico e farmacológico das toxinas do veneno dessa espécie.(AU)


Assuntos
Animais , Venenos de Crotalídeos/toxicidade , /análise , Apoptose , Células Tumorais Cultivadas/citologia , Crotalus , Venenos de Serpentes/toxicidade
2.
Vet. zootec ; 23(3): 465-475, set. 2016. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1503351

Resumo

Las serpientes del género Crotalus ganaron espacio significativo en el campo científico desde el desarrollo de investigaciones con su veneno que involucran la actividad citotóxica. Crotalus durissus cascavella es la única subespecie grabadas del bioma de la Caatinga del nordeste de Brasil y los estudios carecen en sus propiedades del veneno. El presente estudio evaluó el veneno crudo de C. d. cascavella por su actividad citotóxica in vitro contra líneas celulares tumorales y las células normales. El mecanismo de la muerte celular inducida por este veneno también se investigó. C. d. cascavella mostró una alta citotoxicidad (valores de IC50 que oscila desde 2,7 hasta 6,9 g/mL) contra cinco líneas de células tumorales, entre las cuales: OVCAR - 8 y SKOV3 (carcinoma de ovario), PC-3M (carcinoma metastásico de próstata), MCF-7 (carcinoma de mama), SF - 268 (glioblastoma) y PBMC también. Las células tratadas con C. d. cascavella disparó la cascada apoptótica, según lo confirmado por diversos métodos que se muestran en este trabajo. Estos resultados refuerzan así el potencial biomédico y farmacológico de las toxinas del veneno de esta especie.


Snakes from the genus Crotalus gained significant space in the scientific field since the development of researches with their venom involving cytotoxic activity. Crotalus durissus cascavella is the only subspecies recorded of the Caatinga biome of Northeastern Brazil and its noticed a lack of studies on its venom properties. The present study evaluated the crude venom of C. d. cascavella for its cytotoxic activity in vitro against both tumor cell lines and normal cells. The mechanism of cell death induced by this venom was also investigated. C. d. cascavella venom presented high cytotoxicity (IC50 values ranging from 2.7 to 6.9 µg/mL) against five tumor cells lines: OVCAR-8 and SKOV3 (ovarian carcinomas), PC-3M (metastatic prostate carcinoma), MCF-7 (breast carcinoma), and SF-268 (glioblastoma) and PBMC as well. The cells treated with C. d. cascavella venom triggered the apoptotic pathway as confirmed by several methods. These results underline the biomedical potential of toxins from the venom from this species.


Serpentes do Gênero Crotalus ganhou espaço significativo no campo científico, desde o desenvolvimento de pesquisas com seu veneno envolvendo atividade citotóxica. A Crotalus durissus cascavella (C.d.cascavella) é uma subespécie característica da Caatinga do Nordeste do Brasil e faltam estudos sobre as propriedades de seu veneno. O presente estudo avaliou o veneno bruto de C. d. cascavella para a sua atividade citotóxica in vitro contra as linhagens de células tumorais e células normais. O mecanismo de morte celular induzida por este veneno também foi investigado. Observou-se alta citotoxicidade (valores de IC50 entre 2,7-6,9 µg/mL) contra cinco linhagens de células tumorais, dentre elas: OVCAR - 8 e SKOV3 (carcinoma ovariano), PC-3M (carcinoma metastático de próstata), MCF-7 (carcinoma mamário), SF - 268 (glioblastoma) e PBMC. As células tratadas dispararam a cascata apoptótica, como confirmado por vários métodos demonstrados neste trabalho. Esses resultados reforçam, assim, o potencial biomédico e farmacológico das toxinas do veneno dessa espécie.


Assuntos
Animais , Apoptose , Células Tumorais Cultivadas/citologia , Venenos de Crotalídeos/toxicidade , Crotalus , Venenos de Serpentes/toxicidade
3.
Acta sci. vet. (Online) ; 43: Pub. 1301, July 31, 2015. ilus
Artigo em Inglês | VETINDEX | ID: vti-24324

Resumo

Background: Mammary remodeling is determined by a combination of cell differentiation, proliferation and programmeddeath controlled not only by systemic hormones, but also by proteins produced either in the stromal or in the epithelialcompartments. However, few works were undertaken to use phenotypic markers for cellular components of the lactatingcaprine mammary gland and to determine structural–functional relationships. Thus, the aim of this research was to evaluate the morphology and α-SMA, F-actin and JC1 protein expression in the mammary gland of Canindé goats in earlyhormonal lactation.Materials, Methods & Results: Fourteen 2 years old female Canindé goats were used and distributed into two groups: nonlactating (n = 4) and lactating animals (n = 10). Lactation was induced by using estrogen, progesterone and prednisoloneaccording to previous protocol. All subjects were housed indoors and had four hours of daily access to solarium. Mammarygland biopsies were obtained at days 5 (D5) and 26 (D26) of the early stage of lactation and were assessed by histologicaland immunohistochemical analysis. The microstructure of mammary gland in lactating goats was analyzed by conventionalhistologic techniques. Immunohistochemistry was used by identify α-SMA in myoepithelial cells and JC1 in epithelialcells. α-SMA and F-actin expression were assessed by confocal microscopy. Concerning microscopic features, the alveolistructure was evaluated in respect to number, size and cell population. The structural units of the lactating mammary glandconsisted of variably shaped lobules. When comparing to lactating tissue, sections of the non-lactating mammary glandshowed a lower number and size of alveoli, separated by wide connective tissue. Lactating tissues demonstrated numerousand well-developed alveoli, separated by thin trabeculae of connective tissue. In relation to lactating goats, no difference (P> 0.05)...(AU)


Assuntos
Animais , Feminino , Actinas/análise , Glândulas Mamárias Animais/fisiologia , Células Epiteliais , Proteína Quinase 1 Ativada por Mitógeno , Cabras
4.
Acta sci. vet. (Impr.) ; 43: Pub.1301-2015. ilus
Artigo em Inglês | VETINDEX | ID: biblio-1457339

Resumo

Background: Mammary remodeling is determined by a combination of cell differentiation, proliferation and programmeddeath controlled not only by systemic hormones, but also by proteins produced either in the stromal or in the epithelialcompartments. However, few works were undertaken to use phenotypic markers for cellular components of the lactatingcaprine mammary gland and to determine structural–functional relationships. Thus, the aim of this research was to evaluate the morphology and α-SMA, F-actin and JC1 protein expression in the mammary gland of Canindé goats in earlyhormonal lactation.Materials, Methods & Results: Fourteen 2 years old female Canindé goats were used and distributed into two groups: nonlactating (n = 4) and lactating animals (n = 10). Lactation was induced by using estrogen, progesterone and prednisoloneaccording to previous protocol. All subjects were housed indoors and had four hours of daily access to solarium. Mammarygland biopsies were obtained at days 5 (D5) and 26 (D26) of the early stage of lactation and were assessed by histologicaland immunohistochemical analysis. The microstructure of mammary gland in lactating goats was analyzed by conventionalhistologic techniques. Immunohistochemistry was used by identify α-SMA in myoepithelial cells and JC1 in epithelialcells. α-SMA and F-actin expression were assessed by confocal microscopy. Concerning microscopic features, the alveolistructure was evaluated in respect to number, size and cell population. The structural units of the lactating mammary glandconsisted of variably shaped lobules. When comparing to lactating tissue, sections of the non-lactating mammary glandshowed a lower number and size of alveoli, separated by wide connective tissue. Lactating tissues demonstrated numerousand well-developed alveoli, separated by thin trabeculae of connective tissue. In relation to lactating goats, no difference (P> 0.05)...


Assuntos
Feminino , Animais , Actinas/análise , Cabras , Células Epiteliais , Glândulas Mamárias Animais/fisiologia , Proteína Quinase 1 Ativada por Mitógeno
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