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1.
Rev. bras. ciênc. avic ; 25(1): eRBCA-2022-1628, 2023. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1416205

Resumo

Salmonellosis is an important gastrointestinal infection in humans and cause of foodborne outbreaks in the world. In this context, molecular characterization is essential to understand how the strains circulate. The aim of this study was to evaluate the genotypic distribution of S. Heidelberg according to the source of isolation. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE). The most prevalent pulsotypes of cluster A were BRJF6X01.006 (27/95 = 28,42%) related between 1995 and 2011 in broilers, poultry meat and poultry farms, meat product and human, and BRJF6X01.001 (21/95 = 22,10%) related between 2011 and 2017 in wild animals, broilers, poultry meat, poultry farms, meat product, animal feed, and pork meat. The pulsotype BRJF6X01.001 shows a high distribution in the environmental and productive chain. The degree of similarity between pulsotypes BRJF6X01.006 and BRJF6X01.001 is 88%. To ensure the safety of human and animal health, holistic approaches, including surveillance of Salmonella throughout the environment and in the production chain, together with control measures, are critical. As transmission of Salmonella from food producing animals to wildlife and to the environment is considered potential public health problem, information on the survival and persistence of Salmonella in the environment and in potential reservoirs is of considerable importance.(AU)


Assuntos
Humanos , Animais , Bovinos , Aves Domésticas/microbiologia , Salmonella/isolamento & purificação , Salmonelose Animal/genética , Aves/microbiologia , Animais Selvagens/microbiologia , Brasil , Eletroforese em Gel de Campo Pulsado/métodos
2.
R. bras. Ci. avíc. ; 22(1): eRBCA-2019-1196, 2020. tab
Artigo em Inglês | VETINDEX | ID: vti-27479

Resumo

The present study investigated the frequency, level of contamination and serotyping of Salmonella strains isolated from broiler flocks in different processing sites and the fulfillment of a Performance Objective (PO) in frozen chicken breasts, as a risk assessment to measure the efficacy of prevention and control programs applied to reduce the risk of Salmonella spp. in raw poultry meat that contribute to reach food safety and public health goals. From 1,800 samples of cloacal swabs, carcasses before and after immersion chilling and frozen breasts derived from 20 broiler flocks slaughtered at two processing plants located in the mid-west and southern regions of Brazil, 278 samples were positive for Salmonella spp. by polymerase chain reaction (PCR) automated BAX System (DUPONT QUALICOM, USA), and 118 were enumerated by miniaturized most probable number technique. 122 Salmonella spp. strains were serotyped at the National Reference Laboratory of Cholera and Enteric Diseases of Oswaldo Cruz Institute Foundation (FIOCRUZ), showing a dominance of Salmonella Minnesota in every processing steps of the slaughterhouse located in the Brazilian mid-west region. Only 1 lot failed to reach the expected result for the Performance Objective (PO), using a maximum of 10% positivity acceptance for Salmonella spp. in frozen chicken breasts. Qualitative and quantitative results combined may be considered an effective tool to evaluate the effect of prevention and control programs for Salmonella spp. on the safety of the final product.(AU)


Assuntos
Animais , Galinhas/microbiologia , Infecções por Salmonella , Desenvolvimento Tecnológico/análise
3.
Rev. bras. ciênc. avic ; 22(1): eRBCA, 2020. tab
Artigo em Inglês | VETINDEX | ID: biblio-1490750

Resumo

The present study investigated the frequency, level of contamination and serotyping of Salmonella strains isolated from broiler flocks in different processing sites and the fulfillment of a Performance Objective (PO) in frozen chicken breasts, as a risk assessment to measure the efficacy of prevention and control programs applied to reduce the risk of Salmonella spp. in raw poultry meat that contribute to reach food safety and public health goals. From 1,800 samples of cloacal swabs, carcasses before and after immersion chilling and frozen breasts derived from 20 broiler flocks slaughtered at two processing plants located in the mid-west and southern regions of Brazil, 278 samples were positive for Salmonella spp. by polymerase chain reaction (PCR) automated BAX System (DUPONT QUALICOM, USA), and 118 were enumerated by miniaturized most probable number technique. 122 Salmonella spp. strains were serotyped at the National Reference Laboratory of Cholera and Enteric Diseases of Oswaldo Cruz Institute Foundation (FIOCRUZ), showing a dominance of Salmonella Minnesota in every processing steps of the slaughterhouse located in the Brazilian mid-west region. Only 1 lot failed to reach the expected result for the Performance Objective (PO), using a maximum of 10% positivity acceptance for Salmonella spp. in frozen chicken breasts. Qualitative and quantitative results combined may be considered an effective tool to evaluate the effect of prevention and control programs for Salmonella spp. on the safety of the final product.


Assuntos
Animais , Desenvolvimento Tecnológico/análise , Galinhas/microbiologia , Infecções por Salmonella
4.
R. bras. Ci. avíc. ; 22(1): eRBCA-2019-1206, 2020. tab
Artigo em Inglês | VETINDEX | ID: vti-29142

Resumo

The extensive use of antimicrobial agents has contributed to the emergence of antimicrobial resistance and multidrug resistance (MDR) in Salmonella, an important zoonotic pathogen that causes outbreaks and sporadic cases of gastroenteritis in humans. The study aimed to investigate the antimicrobial resistance profile of Salmonella strains isolated from poultry in Brazil. A total of 230 Salmonella strains, isolated from cloacal swabs (n=56) and broiler carcasses swabs (n=174) before and after chilling from slaughterhouses under Federal Inspection Service within the period 2012-2017, were analyzed. Serotyping and antibiotic susceptibility testing were performed on all the isolates. Serotyping results showed that 41% of the strains were Salmonella Heidelberg, 29% S. Minnesota, 12% S. Saintpaul, 6.5% S. Enteritidis, 3.9% S. Anatum, 2.2% S. Cerro, 2.2% S. Senftenberg, 1.7% S. Newport, 0.4% S. Ealing, 0.4% S. O:4,5 and 0.4% S. O:9,12. MDR rates of the isolates were 67.4%. S. Heidelberg 89.5%, S. Minnesota 51.5%, S. Saintpaul 82.1%, S. Anatum 66.7%, S. Cerro 60%, S. Senftenberg 40%. Out of the 230 strains, 41.3% presented resistance to Penicillins + beta-lactamase inhibitor, Penicillin, 1st and 2nd Generation Cephalosporin, 3rd and 4th Generation Cephalosporin, Tetracycline and Sulfonamide. Salmonella Heidelberg, S. Saintpaul, S. Anatum, S. Cerro, S. Senftenberg and S. Minnesota were isolated after chilling tank highlighting a food safety concern for the industry of poultry and poultry products indicating a risk to collective health. The high prevalence of MDR nontyphoidal Salmonella obtained in this study limit the options available to treat infectious disease in humans and animals.(AU)


Assuntos
Animais , Salmonella/imunologia , Prevalência , Galinhas/microbiologia , Anti-Infecciosos
5.
Rev. bras. ciênc. avic ; 22(1): eRBCA, 2020. tab
Artigo em Inglês | VETINDEX | ID: biblio-1490745

Resumo

The extensive use of antimicrobial agents has contributed to the emergence of antimicrobial resistance and multidrug resistance (MDR) in Salmonella, an important zoonotic pathogen that causes outbreaks and sporadic cases of gastroenteritis in humans. The study aimed to investigate the antimicrobial resistance profile of Salmonella strains isolated from poultry in Brazil. A total of 230 Salmonella strains, isolated from cloacal swabs (n=56) and broiler carcasses swabs (n=174) before and after chilling from slaughterhouses under Federal Inspection Service within the period 2012-2017, were analyzed. Serotyping and antibiotic susceptibility testing were performed on all the isolates. Serotyping results showed that 41% of the strains were Salmonella Heidelberg, 29% S. Minnesota, 12% S. Saintpaul, 6.5% S. Enteritidis, 3.9% S. Anatum, 2.2% S. Cerro, 2.2% S. Senftenberg, 1.7% S. Newport, 0.4% S. Ealing, 0.4% S. O:4,5 and 0.4% S. O:9,12. MDR rates of the isolates were 67.4%. S. Heidelberg 89.5%, S. Minnesota 51.5%, S. Saintpaul 82.1%, S. Anatum 66.7%, S. Cerro 60%, S. Senftenberg 40%. Out of the 230 strains, 41.3% presented resistance to Penicillins + beta-lactamase inhibitor, Penicillin, 1st and 2nd Generation Cephalosporin, 3rd and 4th Generation Cephalosporin, Tetracycline and Sulfonamide. Salmonella Heidelberg, S. Saintpaul, S. Anatum, S. Cerro, S. Senftenberg and S. Minnesota were isolated after chilling tank highlighting a food safety concern for the industry of poultry and poultry products indicating a risk to collective health. The high prevalence of MDR nontyphoidal Salmonella obtained in this study limit the options available to treat infectious disease in humans and animals.


Assuntos
Animais , Anti-Infecciosos , Galinhas/microbiologia , Prevalência , Salmonella/imunologia
6.
Arq. bras. med. vet. zootec. (Online) ; 70(5): 1645-1648, set.-out. 2018. graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-947786

Resumo

O objetivo do estudo foi investigar a prevalência de Salmonella spp. em amostras de fezes de búfalos do estado de São Paulo, Brasil, e identificar os sorotipos isolados. Foram examinadas 116 amostras de suabes retais de búfalos das raças Jafarabadi e Murrah, coletadas em triplicata, em seis propriedades rurais localizadas nas regiões Central, Centro-Oeste e Nordeste do estado de São Paulo, Brasil. Para avaliar a presença de Salmonella spp., foram utilizados três diferentes caldos de enriquecimento (caldo selenito cistina, caldo tetrationado Muller-Kauffmann e caldo Rappaport-Vassiliadis) e dois diferentes meios de cultura (ágar verde brilhante modificado e ágar XLT4). Das 116 amostras de suabes retais examinadas, oito amostras (6,90%; 8/116) foram positivas para Salmonella spp., incluindo quatro sorotipos: S. Panama (50%; 4/8), S. Agona (25%; 2/8) , S. Newport (12,5%; 1/8) e S. Saintpaul (12,5%; 1/8), todos isolados de búfalos sem sinais clínicos de salmonelose, indicando a importância dos animais assintomáticos como fonte de infecção para outros animais e seres humanos. Das seis propriedades rurais avaliadas, apenas em duas fazendas (33,3%; 2/6) não foi detectada Salmonella spp. O uso de mais de um caldo de enriquecimento seletivo e de mais de um meio de cultura é indicado para o isolamento de Salmonella.(AU)


Assuntos
Animais , Búfalos/microbiologia , Salmonella/classificação , Sorogrupo
7.
Arq. bras. med. vet. zootec. (Online) ; 70(5): 1645-1648, set.-out. 2018. graf
Artigo em Inglês | VETINDEX | ID: vti-20624

Resumo

O objetivo do estudo foi investigar a prevalência de Salmonella spp. em amostras de fezes de búfalos do estado de São Paulo, Brasil, e identificar os sorotipos isolados. Foram examinadas 116 amostras de suabes retais de búfalos das raças Jafarabadi e Murrah, coletadas em triplicata, em seis propriedades rurais localizadas nas regiões Central, Centro-Oeste e Nordeste do estado de São Paulo, Brasil. Para avaliar a presença de Salmonella spp., foram utilizados três diferentes caldos de enriquecimento (caldo selenito cistina, caldo tetrationado Muller-Kauffmann e caldo Rappaport-Vassiliadis) e dois diferentes meios de cultura (ágar verde brilhante modificado e ágar XLT4). Das 116 amostras de suabes retais examinadas, oito amostras (6,90%; 8/116) foram positivas para Salmonella spp., incluindo quatro sorotipos: S. Panama (50%; 4/8), S. Agona (25%; 2/8) , S. Newport (12,5%; 1/8) e S. Saintpaul (12,5%; 1/8), todos isolados de búfalos sem sinais clínicos de salmonelose, indicando a importância dos animais assintomáticos como fonte de infecção para outros animais e seres humanos. Das seis propriedades rurais avaliadas, apenas em duas fazendas (33,3%; 2/6) não foi detectada Salmonella spp. O uso de mais de um caldo de enriquecimento seletivo e de mais de um meio de cultura é indicado para o isolamento de Salmonella.(AU)


Assuntos
Animais , Búfalos/microbiologia , Salmonella/classificação , Sorogrupo
8.
R. bras. Ci. avíc. ; 19(1): 135-142, jan.-mar. 2017. graf, tab
Artigo em Inglês | VETINDEX | ID: vti-688262

Resumo

The present study investigated the effectiveness of a single Salmonella prevention and control program applied in two different processing plants, located in the states of Mato Grosso do Sul (plant A) and Santa Catarina (Plant B), belonging to the same company, and identified Salmonella strain subtypes isolated from broilers, carcasses before and after chilling, and frozen chicken breasts. The Salmonella prevention and control program was 90% effective in plant A and 100% in plant B, considering a level of 10% positive samples per frozen chicken breast batch acceptable. A total of 128 strains were serotyped, being 10 from drag swabs, 31 from cloacal swabs, 83 from carcasses, and 4 from frozen chicken breasts. After serotyping analysis, 30 strains isolated at different processing steps and drag swabs, and three Salmonella Minnesota strains isolated in 2012 in plant A, were genotyped by PFGE. In plant A, the most frequently strain isolated was Salmonella Minnesota (90.35%), followed by Salmonella Newport (8.77%), and in Plant B, Salmonella Senftenberg (80%). Salmonella Minnesota strains were differentiated by PFGE into 19 pulsotypes distributed in three clusters. The phenotypic identification by serotyping of four strains diverged from their PFGE genotypic results. Most Salmonella Minnesota strains genotyped in plant A and the strains isolated from environmental samples in 2012 in the same broiler processing plant belong to a single cluster, confirming the dominance and persistence of this clone over time.(AU)


Assuntos
Animais , Salmonella/genética , Galinhas/anormalidades , Galinhas/genética , Abastecimento de Alimentos , Técnicas de Genotipagem/veterinária
9.
Rev. bras. ciênc. avic ; 19(1): 135-142, jan.-mar. 2017. graf, tab
Artigo em Inglês | VETINDEX | ID: biblio-1490356

Resumo

The present study investigated the effectiveness of a single Salmonella prevention and control program applied in two different processing plants, located in the states of Mato Grosso do Sul (plant A) and Santa Catarina (Plant B), belonging to the same company, and identified Salmonella strain subtypes isolated from broilers, carcasses before and after chilling, and frozen chicken breasts. The Salmonella prevention and control program was 90% effective in plant A and 100% in plant B, considering a level of 10% positive samples per frozen chicken breast batch acceptable. A total of 128 strains were serotyped, being 10 from drag swabs, 31 from cloacal swabs, 83 from carcasses, and 4 from frozen chicken breasts. After serotyping analysis, 30 strains isolated at different processing steps and drag swabs, and three Salmonella Minnesota strains isolated in 2012 in plant A, were genotyped by PFGE. In plant A, the most frequently strain isolated was Salmonella Minnesota (90.35%), followed by Salmonella Newport (8.77%), and in Plant B, Salmonella Senftenberg (80%). Salmonella Minnesota strains were differentiated by PFGE into 19 pulsotypes distributed in three clusters. The phenotypic identification by serotyping of four strains diverged from their PFGE genotypic results. Most Salmonella Minnesota strains genotyped in plant A and the strains isolated from environmental samples in 2012 in the same broiler processing plant belong to a single cluster, confirming the dominance and persistence of this clone over time.


Assuntos
Animais , Abastecimento de Alimentos , Galinhas/anormalidades , Galinhas/genética , Salmonella/genética , Técnicas de Genotipagem/veterinária
10.
Arq. bras. med. vet. zootec. (Online) ; 68(1): 39-47, jan.-fev. 2016. tab
Artigo em Português | VETINDEX | ID: vti-334143

Resumo

Foi analisado um total de 1824 cepas de Salmonella, isoladas de alimentos de origem suína, no período de janeiro/2005 a junho/2010. As cepas, provenientes de diferentes regiões do país, foram recebidas pelo Labent/IOC/FIocruz para caracterização antigênica conclusiva. Foram identificados 41 sorovares, destacando-se: Typhimurium, Derby, Enteritidis, Panama, Infantis e Anatum. Aspectos bacteriológicos e epidemiológicos relacionados a esses sorovares foram discutidos. O teste de suscetibilidade aos antimicrobianos foi realizado em 357 amostras, 257 (72%) foram resistentes a uma ou mais drogas, e destas, 31,9% mostraram-se multirresistentes. A variedade de sorovares observada neste estudo confirma o papel dos suínos na cadeia alimentar como importantes reservatórios de Salmonella, agravado ainda pelo elevado percentual de cepas resistentes a um ou mais antimicrobianos, alertando para uma condição de risco à saúde pública.(AU)


We analyzed a total of 1824 strains of Salmonella isolated from swine-origin foods from January/2005 to June/2010. The strains from different regions of the country were received by Labent/IOC/FIOCRUZ for conclusive antigenic characterization. We identified 41 serovars, of which these stood out: Typhimurium, Derby, Enteritidis, Panama, Infantis and Anatum. Bacteriological and epidemiological aspects related to these serovars were discussed. The antimicrobial susceptibility test was performed on 357 samples, 257 (72%) were resistant to one or more of these drugs and 31,9% were multiresistant. A variety of serovars were identified reinforcing the swine as an important reservoir of Salmonella in the food chain. The high rates of antimicrobial resistance obtained in this evaluation may represent a risk condition to human health.(AU)


Assuntos
Animais , Salmonella , Zoonoses , Saúde Pública Veterinária , Resistência Microbiana a Medicamentos , Doenças dos Suínos , Epidemiologia , Sorologia , Anti-Infecciosos
11.
Arq. bras. med. vet. zootec ; 61(4): 1000-1001, ago. 2009.
Artigo em Português | VETINDEX | ID: vti-6405

Resumo

A total of 30 stool samples from 13 healthy dogs were analyzed in order to detect microorganisms in their gastrointestinal tract. The samples were enriched in alkaline peptone water added with 1 per cent and 3 per cent sodium chloride and Rappaport-Vassiliadis enrichment broth (37ºC/18-24h). Then, they were streaked onto Pseudomonas-Aeromonas selective agar base, tiossulfate citrate bile sucrose agar, eosin methylene blue agar, and Hektoen enteric agar. After the biochemical characterization, the results showed 41 bacterial isolated strains, from which Escherichia coli, Vibrio fluvialis, and Aeromonas caviae were the main pathogens encountered.(AU)


Assuntos
Animais , Trato Gastrointestinal/patologia , Interações Hospedeiro-Patógeno , Fezes/microbiologia , Cães/parasitologia , Saúde Pública Veterinária
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