Healing of skin wounds in rats: influence of low-level laser therapy, alone or combined with natural products

Background: A great variety of natural products have been evaluated for the skin wound healing due to anti-inflammatory,antioxidant and antibacterial activities, and procollagen synthesis, of which may be mentioned the honey and propolis. Inturn, low-level laser therapy (LLLT) is considered an important tool in the treatment of skin wounds, because of ability toraise the ATP production, to stimulate the microcirculation, and formation of new blood vessels. Therefore, this study aimedto assess the influence of LLLT, alone or combined with natural products, in the healing of excisional skin wounds in rats.Materials, Methods & Results: One hundred twenty-six male Wistar rats, aged 14 weeks, were randomly divided intoseven groups (n = 18 per group) according to wound treatments: G1 (negative control): 0.9% saline solution; G2 (positive control): allantoin + zinc oxide ointment; G3: LLLT; G4: mixture of honey + 5% propolis hydroalcoholic solution;G5: LLLT + mixture of honey + hydroalcoholic solution of 5% propolis; G6: LLLT + honey in natura; G7: LLLT + 5%propolis hydroalcoholic solution. Six rats from each group were euthanized on the 7th, 14th, and 21th postoperative days.Macroscopic and histological evaluations of wound healing were performed. A decrease in wound area occurred in allgroups, but in general G4 had the higher reduction and G1 and G3 had the lower reductions. These findings correspondedto the percentage of wound contraction, since the largest contraction was G4 and the lowest contractions were G1 andG3. Histological analysis showed no significant difference among groups...

Healing of skin wounds in rats: influence of low-level laser therapy, alone or combined with natural products

Background: A great variety of natural products have been evaluated for the skin wound healing due to anti-inflammatory,antioxidant and antibacterial activities, and procollagen synthesis, of which may be mentioned the honey and propolis. Inturn, low-level laser therapy (LLLT) is considered an important tool in the treatment of skin wounds, because of ability toraise the ATP production, to stimulate the microcirculation, and formation of new blood vessels. Therefore, this study aimedto assess the influence of LLLT, alone or combined with natural products, in the healing of excisional skin wounds in rats.Materials, Methods & Results: One hundred twenty-six male Wistar rats, aged 14 weeks, were randomly divided intoseven groups (n = 18 per group) according to wound treatments: G1 (negative control): 0.9% saline solution; G2 (positive control): allantoin + zinc oxide ointment; G3: LLLT; G4: mixture of honey + 5% propolis hydroalcoholic solution;G5: LLLT + mixture of honey + hydroalcoholic solution of 5% propolis; G6: LLLT + honey in natura; G7: LLLT + 5%propolis hydroalcoholic solution. Six rats from each group were euthanized on the 7th, 14th, and 21th postoperative days.Macroscopic and histological evaluations of wound healing were performed. A decrease in wound area occurred in allgroups, but in general G4 had the higher reduction and G1 and G3 had the lower reductions. These findings correspondedto the percentage of wound contraction, since the largest contraction was G4 and the lowest contractions were G1 andG3. Histological analysis showed no significant difference among groups...(AU)

Femoral diaphyseal fractures fixation technique using an adjustable nylon tie in dog (Canis lupus familiaris) and cat (Felis catus domesticus)

Background: Adjustable nylon ties polyamide 6.6 is devices produced from the same material of surgical nylon wire and have been used in different surgical procedures in small animals and in human patient. Reports regarding the use of these devices as secondary fixation technique of femoral diaphyseal fractures in animals are rare in the literature. The aim of the present report case was to describe the use of adjustable nylon tie polyamide 6.6 as secondary fixation technique in a 3-year-old dog and 4-month-old cat, diagnosed with femoral diaphyseal fractures.Cases: Case 1. A 3-year-old female dog was presented with reluctance to support the left hind limb, with 5 days’ duration. Pain and edema on the left femoral diaphyseal region was identified. The limb was submitted to radiographic exam and revealed a closed, complete and comminuted fracture of the diaphysis of the femur, and was decided to perform a surgical stabilization by open reduction through primary fixation with intramedullary pin, and secondary fixation of bone fragments with adjustable nylon ties polyamide 6.6, as a substitute of steel cerclage wire. The bone fragments were alignment and fixated with five polyamide nylon ties. The excess was removed with a scalpel blade along the lock. Seven days after surgery the skin sutures were removed and were observed reluctance to support the left hind limb. Physiotherapy sessions were prescribed. Forty days after the surgery was performed a radiographic exam of the left hind limb which revealed alignment of the bone axis. Six months after the surgery, the owner reported that the dog supported the left hind limb. Case 2. A 4-month-old female cat was presented with history of trauma, with 24 h’s duration, and reluctance in supporting the left hind limb. On physical examination there was identified pain on the left femoral diaphyseal region.[...]

Femoral diaphyseal fractures fixation technique using an adjustable nylon tie in dog (Canis lupus familiaris) and cat (Felis catus domesticus)

Background: Adjustable nylon ties polyamide 6.6 is devices produced from the same material of surgical nylon wire and have been used in different surgical procedures in small animals and in human patient. Reports regarding the use of these devices as secondary fixation technique of femoral diaphyseal fractures in animals are rare in the literature. The aim of the present report case was to describe the use of adjustable nylon tie polyamide 6.6 as secondary fixation technique in a 3-year-old dog and 4-month-old cat, diagnosed with femoral diaphyseal fractures.Cases: Case 1. A 3-year-old female dog was presented with reluctance to support the left hind limb, with 5 days duration. Pain and edema on the left femoral diaphyseal region was identified. The limb was submitted to radiographic exam and revealed a closed, complete and comminuted fracture of the diaphysis of the femur, and was decided to perform a surgical stabilization by open reduction through primary fixation with intramedullary pin, and secondary fixation of bone fragments with adjustable nylon ties polyamide 6.6, as a substitute of steel cerclage wire. The bone fragments were alignment and fixated with five polyamide nylon ties. The excess was removed with a scalpel blade along the lock. Seven days after surgery the skin sutures were removed and were observed reluctance to support the left hind limb. Physiotherapy sessions were prescribed. Forty days after the surgery was performed a radiographic exam of the left hind limb which revealed alignment of the bone axis. Six months after the surgery, the owner reported that the dog supported the left hind limb. Case 2. A 4-month-old female cat was presented with history of trauma, with 24 hs duration, and reluctance in supporting the left hind limb. On physical examination there was identified pain on the left femoral diaphyseal region.[...](AU)