Comparação entre métodos de diagnóstico de Cryptosporidium spp. em amostras fecais de bovinos / Comparison between diagnostic methods of Cryptosporidium spp. in fecal samples from cattle

Criptosporidiose é uma doença entérica com manifestações clínicas variadas e eventual mortalidade, principalmente em animais jovens, causando prejuízos ao desenvolvimento. Este estudo objetivou comparar técnicas de coloração para a detecção de oocistos de Cryptosporidium spp. em fezes de bezerros leiteiros, provenientes dos 22 municípios da região Sul do Rio Grande do Sul, Brasil. Amostras fecais foram colhidas diretamente do reto de 359 bezerros de diferentes raças, machos e fêmeas, com até doze meses de idade. Oocistos de Cryptosporidium spp. foram observados por meio dos métodos colorimétricos de Kinyoun, Safranina e Ziehl-Neelsen e visualizados sob microscopia ótica e porfluorescência com coloração de Auramina. Oocistos de Cryptosporidium spp. Foram observados em 6,69% (24/359) das amostras analisadas. A partir destes resultados pode-se inferir que os quatros métodos colorimétricos, foram eficazes na detecção de Cryptosporidium spp., sendo capaz de revelar este parasito mesmo em amostras com reduzido número de oocistos. Não houve diferença estatisticamente significante entre os métodos para o diagnóstico, apesar do método de fluorescência com coloração de Auramina ter apresentado o melhor resultado em comparação com as técnicas colorimétricas utilizadas neste estudo.

Cryptosporidiosis is an enteric disease with varied clinical manifestations and eventual mortality, mainly in young animals, causing impairment in development. This study aimed to compare staining techniques for the detection of Cryptosporidium spp. oocysts in dairy calf feces from 22 municipalities of the southern region of Rio Grande do Sul, Brazil. Fecal samples were collected directly from the rectum of 359 calves of different breeds ,male and female, up to twelve months old. Cryptosporidium spp. oocysts were observed using the colorimetric methods of Kinyoun, Safranina and Ziehl-Neelsen and were visualized under optical microscopy and by fluorescence with auramine stain. Cryptosporidium spp. oocysts were observed in 6.69% (24/359) of the analyzed samples. From these results it can be inferred that the four colorimetric methods were effective in the detection of Cryptosporidium spp., being able to reveal this parasite even in samples with a reduced number of oocysts. There was no statistically significant difference between the methods for diagnosis, even though, the Auramine staining fluorescence method presented the best result in comparison with the colorimetric techniques used in this study.

Raiva paralítica em suíno no Rio Grande do Sul / Paralytic rabies in swine at Rio Grande do Sul

A raiva é uma doença infecciosa, potencialmente zoonótica, de distribuição mundial que afeta mamíferos domésticos, silvestres e humanos. Na América do Sul a raiva paralítica ocorre na forma de surtos cíclicos e é transmitida por morcegos hematófagos, principalmente o Desmodus rotundus. É uma doença responsável por prejuízos econômicos na pecuária, além de apresentar importância para a saúde pública. O presente trabalho tem como objetivo relatar um caso de raiva suína diagnosticado no Laboratório Regional de Diagnóstico, da Faculdade de Veterinária, da Universidade Federal de Pelotas. O caso ocorreu em uma propriedade localizada no município do Capão do Leão em um suíno, fêmea, sem raça definida, de dois anos de idade, criada em regime semiextensivo. Segundo o proprietário o animal apresentou apatia, anorexia, paralisia progressiva e decúbito lateral. Devido a evolução do quadro clínico foi realizada a eutanásia. Na necropsia não foram observadas lesões significativas. No exame histopatológico do encéfalo observou-se meningoencefalite não supurativa, manguito perivascular de linfócitos e raros eosinófilos, além de satelitose e gliose. Fragmentos do encéfalo e medula foram submetidos ao exame de Imunofluorescência Direta e prova biológica, confirmando o diagnóstico de raiva.

Rabies is a potentially zoonotic infectious disease with worldwide distribution that affects domestic, wild and human mammals. In South America, paralytic rabies occurs in the form of cyclic outbreaks and is transmitted by hematophagous bats, mainly Desmodus rotundus. It is a disease responsible for economic losses in livestock, besides of presenting importance to public health. This paper aims to report a case of swine rabies diagnosed at the Laboratório Regional de Diagnostico, Faculdade de Veterinária, Universidade Federal de Pelotas. The case occurred in a property located in the county of Capão do Leão in a female pig, twoyear-old, undefined breed, raised in a semi-extensive regime. According to the owner, the animal presented apathy, anorexia, progressive paralysis and lateral decubitus. Due to the evolution of the clinical condition, euthanasia was performed. At necropsy no significant lesions were observed. Histopathological examination of the brain showed non-suppurative meningoencephalitis, perivascular cuff of lymphocytes and rare eosinophils, as well as satelitosis and gliosis. Brain and spinal cord fragments were submitted to Direct Immunofluorescence and biological tests, confirming the diagnosis of rabies.

Cranioesophageal pythiosis in a horse

Background: Pythiosis is a chronic inflammatory disease that is caused by oomycete Pythium insidiosum. This illness affects several species including humans and horses. Equine is the most affected species, having no predisposition for breed, gender, or age. It is usually shown in cutaneous and subcutaneous forms, and the lesions, which grow quickly and are hard to treat, are located mainly in the extremities. The diagnosis is made via epidemiology, clinical signs, and macroscopic and microscopic aspects of the lesion. This study describes a case of cranioesophageal pythiosis in a horse, examining the epidemiological, clinical and pathological characteristics. Case: A 12-year-old male quarter horse, weighing 515 kg was taken to the Veterinary Hospital at the University Center of Espírito Santo (UNESC). The horse had an increase in volume in the cranioesophageal region, coughing, difficulty breathing, and a runny nose. On clinical examination, the horse showed an enlargement in the submandibular and retropharyngeal lymph nodes, subcutaneous edema in the larynx region, and a temperature of 38.2ºC. According to the owner, cough was recurrent and had lasted about 12 months even after treatment with different kinds of antimicrobials. On radiographic exam, there was a marked decrease in the tracheal lumen and increased soft tissue radiopacity in the region adjacent to the narrowing. The animal was taken to surgery to remove the mass, but he died because of complications during surgery. The animal’s owner did not allow necropsy, but a fragment of the mass in the cranioesophageal was removed and sent for histological examination. The fragment was fixed in 10% formalin and processed using routine histological analysis. Macroscopically, the mass was light yellowish and ulcerated, and it measured 7.0 × 5.0 × 5.0 cm. In the middle of the ulcerated areas, there were yellow and...

Comparação entre métodos de diagnóstico de Cryptosporidium spp. em amostras fecais de bovinos / Comparison between diagnostic methods of Cryptosporidium spp. in fecal samples from cattle

Criptosporidiose é uma doença entérica com manifestações clínicas variadas e eventual mortalidade, principalmente em animais jovens, causando prejuízos ao desenvolvimento. Este estudo objetivou comparar técnicas de coloração para a detecção de oocistos de Cryptosporidium spp. em fezes de bezerros leiteiros, provenientes dos 22 municípios da região Sul do Rio Grande do Sul, Brasil. Amostras fecais foram colhidas diretamente do reto de 359 bezerros de diferentes raças, machos e fêmeas, com até doze meses de idade. Oocistos de Cryptosporidium spp. foram observados por meio dos métodos colorimétricos de Kinyoun, Safranina e Ziehl-Neelsen e visualizados sob microscopia ótica e porfluorescência com coloração de Auramina. Oocistos de Cryptosporidium spp. Foram observados em 6,69% (24/359) das amostras analisadas. A partir destes resultados pode-se inferir que os quatros métodos colorimétricos, foram eficazes na detecção de Cryptosporidium spp., sendo capaz de revelar este parasito mesmo em amostras com reduzido número de oocistos. Não houve diferença estatisticamente significante entre os métodos para o diagnóstico, apesar do método de fluorescência com coloração de Auramina ter apresentado o melhor resultado em comparação com as técnicas colorimétricas utilizadas neste estudo.(AU)

Cryptosporidiosis is an enteric disease with varied clinical manifestations and eventual mortality, mainly in young animals, causing impairment in development. This study aimed to compare staining techniques for the detection of Cryptosporidium spp. oocysts in dairy calf feces from 22 municipalities of the southern region of Rio Grande do Sul, Brazil. Fecal samples were collected directly from the rectum of 359 calves of different breeds ,male and female, up to twelve months old. Cryptosporidium spp. oocysts were observed using the colorimetric methods of Kinyoun, Safranina and Ziehl-Neelsen and were visualized under optical microscopy and by fluorescence with auramine stain. Cryptosporidium spp. oocysts were observed in 6.69% (24/359) of the analyzed samples. From these results it can be inferred that the four colorimetric methods were effective in the detection of Cryptosporidium spp., being able to reveal this parasite even in samples with a reduced number of oocysts. There was no statistically significant difference between the methods for diagnosis, even though, the Auramine staining fluorescence method presented the best result in comparison with the colorimetric techniques used in this study.(AU)

Cranioesophageal pythiosis in a horse

Background: Pythiosis is a chronic inflammatory disease that is caused by oomycete Pythium insidiosum. This illness affects several species including humans and horses. Equine is the most affected species, having no predisposition for breed, gender, or age. It is usually shown in cutaneous and subcutaneous forms, and the lesions, which grow quickly and are hard to treat, are located mainly in the extremities. The diagnosis is made via epidemiology, clinical signs, and macroscopic and microscopic aspects of the lesion. This study describes a case of cranioesophageal pythiosis in a horse, examining the epidemiological, clinical and pathological characteristics. Case: A 12-year-old male quarter horse, weighing 515 kg was taken to the Veterinary Hospital at the University Center of Espírito Santo (UNESC). The horse had an increase in volume in the cranioesophageal region, coughing, difficulty breathing, and a runny nose. On clinical examination, the horse showed an enlargement in the submandibular and retropharyngeal lymph nodes, subcutaneous edema in the larynx region, and a temperature of 38.2ºC. According to the owner, cough was recurrent and had lasted about 12 months even after treatment with different kinds of antimicrobials. On radiographic exam, there was a marked decrease in the tracheal lumen and increased soft tissue radiopacity in the region adjacent to the narrowing. The animal was taken to surgery to remove the mass, but he died because of complications during surgery. The animals owner did not allow necropsy, but a fragment of the mass in the cranioesophageal was removed and sent for histological examination. The fragment was fixed in 10% formalin and processed using routine histological analysis. Macroscopically, the mass was light yellowish and ulcerated, and it measured 7.0 × 5.0 × 5.0 cm. In the middle of the ulcerated areas, there were yellow and...(AU)

Raiva paralítica em suíno no Rio Grande do Sul / Paralytic rabies in swine at Rio Grande do Sul

A raiva é uma doença infecciosa, potencialmente zoonótica, de distribuição mundial que afeta mamíferos domésticos, silvestres e humanos. Na América do Sul a raiva paralítica ocorre na forma de surtos cíclicos e é transmitida por morcegos hematófagos, principalmente o Desmodus rotundus. É uma doença responsável por prejuízos econômicos na pecuária, além de apresentar importância para a saúde pública. O presente trabalho tem como objetivo relatar um caso de raiva suína diagnosticado no Laboratório Regional de Diagnóstico, da Faculdade de Veterinária, da Universidade Federal de Pelotas. O caso ocorreu em uma propriedade localizada no município do Capão do Leão em um suíno, fêmea, sem raça definida, de dois anos de idade, criada em regime semiextensivo. Segundo o proprietário o animal apresentou apatia, anorexia, paralisia progressiva e decúbito lateral. Devido a evolução do quadro clínico foi realizada a eutanásia. Na necropsia não foram observadas lesões significativas. No exame histopatológico do encéfalo observou-se meningoencefalite não supurativa, manguito perivascular de linfócitos e raros eosinófilos, além de satelitose e gliose. Fragmentos do encéfalo e medula foram submetidos ao exame de Imunofluorescência Direta e prova biológica, confirmando o diagnóstico de raiva.(AU)

Rabies is a potentially zoonotic infectious disease with worldwide distribution that affects domestic, wild and human mammals. In South America, paralytic rabies occurs in the form of cyclic outbreaks and is transmitted by hematophagous bats, mainly Desmodus rotundus. It is a disease responsible for economic losses in livestock, besides of presenting importance to public health. This paper aims to report a case of swine rabies diagnosed at the Laboratório Regional de Diagnostico, Faculdade de Veterinária, Universidade Federal de Pelotas. The case occurred in a property located in the county of Capão do Leão in a female pig, twoyear-old, undefined breed, raised in a semi-extensive regime. According to the owner, the animal presented apathy, anorexia, progressive paralysis and lateral decubitus. Due to the evolution of the clinical condition, euthanasia was performed. At necropsy no significant lesions were observed. Histopathological examination of the brain showed non-suppurative meningoencephalitis, perivascular cuff of lymphocytes and rare eosinophils, as well as satelitosis and gliosis. Brain and spinal cord fragments were submitted to Direct Immunofluorescence and biological tests, confirming the diagnosis of rabies.(AU)