Occurrence of antibodies anti -Toxoplasma gondii, Neospora caninum and Leptospira interrogans in a captive deer herd in Southern Brazil / Ocorrência de anticorpos anti -Toxoplasma gondii, Neospora caninum e Leptospira interrogans em um rebanho de cervídeos de cativeiro do sul do Brasil

A large number of Brazilian zoos keep many endangered species of deer, however, very few disease surveillance studies have been conducted among captive cervids. Blood samples from 32 Brazilian deer (Blastocerus dichotomus, Mazama nana and Mazama americana) kept in captivity at Bela Vista Biological Sanctuary (Foz do Iguaçu, Brazil) were investigated for 10 ruminant pathogens, with the aims of monitoring deer health status and evaluating any potential zoonotic risk. Deer serum samples were tested for Brucella abortus, Leptospira (23 serovars), Toxoplasma gondii, Neospora caninum, bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, foot-and-mouth disease virus, western equine encephalitis virus, eastern equine encephalitis virus and Venezuelan equine encephalitis virus. Antibodies against T. gondii (15.6%), N. caninum (6.2%) and L. interrogans serogroup Serjoe (3.1%) were detected. The serological results for all other infectious agents were negative. The deer were considered to be clinically healthy and asymptomatic regarding any disease. Compared with studies on free-ranging deer, the prevalences of the same agents tested among the captive deer kept at the Sanctuary were lower, thus indicating good sanitary conditions and high-quality management practices at the zoo.(AU)

Um grande número de zoológicos brasileiros abriga espécies de cervídeos ameaçados de extinção, entretanto, estudos de vigilância de doenças em cervídeos de cativeiro são escassos. Amostras de sangue de 32 cervídeos brasileiros (Blastocerus dichotomus, Mazama nana e Mazama americana), mantidos em cativeiro no Refúgio Biológico Bela Vista (Foz do Iguaçu, Brasil), foram investigados para 10 patógenos de ruminantes, visando monitorar o estado de saúde dos cervídeos e avaliar a presença de agentes zoonóticos. As amostras de soro foram testadas para Brucella abortus, Leptospira (23 sorovares), Toxoplasma gondii, Neospora caninum, diarreia viral bovina, rinotraqueíte infecciosa bovina, febre aftosa, encefalomielite equina do oeste, encefalomielite equina do leste e encefalomielite equina venezuelana. Foram detectados anticorpos para T. gondii (15,6%), N. caninum (6,2%) e para L. interrogans sorogrupo Serjoe (3,1%). As sorologias apresentaram resultado negativo para as demais doenças. Os cervídeos foram considerados clinicamente sadios e assintomáticos para doenças. Comparados aos estudos de populações de vida livre, as soroprevalências para os mesmos agentes testados foram menores para os cervídeos mantidos no Refúgio, indicando as boas condições sanitárias e a qualidade das práticas de manejo no zoológico.(AU)

The use of HPLC identification and quantification of isoflavones content in samples obtained in pharmacies - doi: 10.4025/actascibiolsci.v33i1.6876 / The use of HPLC identification and quantification of isoflavones content in samples obtained in pharmacies - doi: 10.4025/actascibiolsci.v33i1.6876

Nowadays, there is a great interest in the research and the production of functional foods, such as isoflavones. Which present proven action on the prevention of health problems, such as cancer and cardiovascular diseases. Considering the importance of soybean supplements standardization, this study aimed identifying and quantifying the isoflavones in products sold at different pharmacies. Isoflavones samples were acquired in six different pharmacies specialized in the production of phytotherapic medications. The isoflavones were extracted, quantified and identified in HPLC. None of the samples, from different pharmacies, presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavones profile, the highest levels found were of daidzein, with up to 66.8 ± 0.09 µg, being the lowest levels the ones of genistein. It is necessary, therefore, the adoption of methods for the standardization and characterization of the raw materials used by different pharmacies. It would assure the isoflavones content, once they are essential for the effects prescribed by the professionals in the medical area.

Nowadays, there is a great interest in the research and the production of functional foods, such as isoflavones. Which present proven action on the prevention of health problems, such as cancer and cardiovascular diseases. Considering the importance of soybean supplements standardization, this study aimed identifying and quantifying the isoflavones in products sold at different pharmacies. Isoflavones samples were acquired in six different pharmacies specialized in the production of phytotherapic medications. The isoflavones were extracted, quantified and identified in HPLC. None of the samples, from different pharmacies, presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavones profile, the highest levels found were of daidzein, with up to 66.8 ± 0.09 µg, being the lowest levels the ones of genistein. It is necessary, therefore, the adoption of methods for the standardization and characterization of the raw materials used by different pharmacies. It would assure the isoflavones content, once they are essential for the effects prescribed by the professionals in the medical area.

The use of HPLC identification and quantification of isoflavonescontent in samples obtained in pharmacies / O uso do HPLC na identificação e quantificação dos teores de isoflavonas em amostras obtidas de farmácias de manipulação

Nowadays, there is a great interest in the research and the production offunctional foods, such as isoflavones. Which present proven action on the prevention of healthproblems, such as cancer and cardiovascular diseases. Considering the importance of soybeansupplements standardization, this study aimed identifying and quantifying the isoflavones inproducts sold at different pharmacies. Isoflavones samples were acquired in six differentpharmacies specialized in the production of phytotherapic medications. The isoflavones wereextracted, quantified and identified in HPLC. None of the samples, from different pharmacies,presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavonesprofile, the highest levels found were of daidzein, with up to 66.8 ± 0.09 μg, being the lowestlevels the ones of genistein. It is necessary, therefore, the adoption of methods for thestandardization and characterization of the raw materials used by different pharmacies. It wouldassure the isoflavones content, once they are essential for the effects prescribed by theprofessionals in the medical area.(AU)

O uso do HPLC na identificação equantificação dos teores de isoflavonas em amostras obtidas de farmácias de manipulação.Atualmente há um grande interesse na pesquisa e produção de alimentos funcionais, como asisoflavonas, que apresentam comprovada ação na prevenção de males, como o câncer e asdoenças cardiovasculares. Considerando a importância da padronização dos suplementos de soja,o objetivo do presente estudo foi de identificar e quantificar as isoflavonas em produtoscomercializados em diferentes farmácias de manipulação. Amostras de isoflavonas foramadquiridas em seis diferentes farmácias especializadas em manipulação de fitoterápicos. Asisoflavonas foram extraídas, identificadas e quantificadas em CLAE. Nenhuma das amostras, dasdiferentes farmácias de manipulação, apresentou os teores de isoflavonas indicado no rótulo, ouseja, 20 mg. Quanto ao perfil de isoflavonas, os maiores teores encontrados nas amostras foi dedaidzeína com até 66,8 ± 0,09 μg, e os menores de genistina. Sendo assim, é necessário quesejam adotados métodos de padronização e caracterização das matérias-primas utilizadas nasdiferentes farmácias de manipulação. Assegurando os teores de isoflavonas, os quais sãoimprescindíveis para os efeitos prescritos pelos profissionais da área médica.(AU)

The use of HPLC identification and quantification of isoflavones content in samples obtained in pharmacies - doi: 10.4025/actascibiolsci.v33i1.6876 / The use of HPLC identification and quantification of isoflavones content in samples obtained in pharmacies - doi: 10.4025/actascibiolsci.v33i1.6876

Nowadays, there is a great interest in the research and the production of functional foods, such as isoflavones. Which present proven action on the prevention of health problems, such as cancer and cardiovascular diseases. Considering the importance of soybean supplements standardization, this study aimed identifying and quantifying the isoflavones in products sold at different pharmacies. Isoflavones samples were acquired in six different pharmacies specialized in the production of phytotherapic medications. The isoflavones were extracted, quantified and identified in HPLC. None of the samples, from different pharmacies, presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavones profile, the highest levels found were of daidzein, with up to 66.8 ± 0.09 µg, being the lowest levels the ones of genistein. It is necessary, therefore, the adoption of methods for the standardization and characterization of the raw materials used by different pharmacies. It would assure the isoflavones content, once they are essential for the effects prescribed by the professionals in the medical area.

Nowadays, there is a great interest in the research and the production of functional foods, such as isoflavones. Which present proven action on the prevention of health problems, such as cancer and cardiovascular diseases. Considering the importance of soybean supplements standardization, this study aimed identifying and quantifying the isoflavones in products sold at different pharmacies. Isoflavones samples were acquired in six different pharmacies specialized in the production of phytotherapic medications. The isoflavones were extracted, quantified and identified in HPLC. None of the samples, from different pharmacies, presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavones profile, the highest levels found were of daidzein, with up to 66.8 ± 0.09 µg, being the lowest levels the ones of genistein. It is necessary, therefore, the adoption of methods for the standardization and characterization of the raw materials used by different pharmacies. It would assure the isoflavones content, once they are essential for the effects prescribed by the professionals in the medical area.