Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros

Intervalo de ano de publicação
1.
Arq. bras. med. vet. zootec ; 64(4): 1077-1080, Aug. 2012. tab
Artigo em Português | VETINDEX | ID: biblio-1373122

Resumo

The fast agglutination screening test, using S. Typhimurium as the antigen, was compared with the standard bacterial method to identify rheas (Rhea americana) contaminated with Salmonella spp.at slaughter.Seventy birds were serologically tested for Salmonella enterica Pullorum using a commercial antigen.Of these, 66 were also submitted to a macroagglutination test, using a strain of Salmonella Typhimurium isolated from rheas.All birds did not react with the commercial S. Pullorum antigen, but 37 were positive for the FAST-ST.The isolation of Salmonella spp. was verified in 66 (94.2%) birds. 85.7% were found in liver samples, 60% in feces and 42.3% in cloacal swabs.A total of 16.6% were identified as being S. enterica enterica rugosa, 35.9% as S. Typhimurium, 46.5% as S.Newport and 0.9% as S. Anatum. An insignificant concordance between the results of bacterial isolation and the serological response was observed (k=0.016).The detection of Salmonella spp. by bacteriological and serological methods in samples from rheas must be deemed important, because birds without a clinical manifestation can be significant sources of salmonellas in food infections.


Assuntos
Animais , Salmonella/isolamento & purificação , Salmonelose Animal/diagnóstico , Struthioniformes/microbiologia , Testes de Aglutinação/veterinária
2.
Artigo em Português | VETINDEX | ID: vti-447886

Resumo

The fast agglutination screening test, using S. Typhimurium as the antigen, was compared with the standard bacterial method to identify rheas (Rhea americana) contaminated with Salmonella spp.at slaughter.Seventy birds were serologically tested for Salmonella enterica Pullorum using a commercial antigen.Of these, 66 were also submitted to a macroagglutination test, using a strain of Salmonella Typhimurium isolated from rheas.All birds did not react with the commercial S. Pullorum antigen, but 37 were positive for the FAST-ST.The isolation of Salmonella spp. was verified in 66 (94.2%) birds. 85.7% were found in liver samples, 60% in feces and 42.3% in cloacal swabs.A total of 16.6% were identified as being S. enterica enterica rugosa, 35.9% as S. Typhimurium, 46.5% as S.Newport and 0.9% as S. Anatum. An insignificant concordance between the results of bacterial isolation and the serological response was observed (k=0.016).The detection of Salmonella spp. by bacteriological and serological methods in samples from rheas must be deemed important, because birds without a clinical manifestation can be significant sources of salmonellas in food infections.

3.
Arq. bras. med. vet. zootec ; 64(6): 1755-1760, 2012. tab
Artigo em Português | VETINDEX | ID: vti-10873

Resumo

Foram testados os efeitos de dois níveis de extrato etéreo à base de óleo de arroz no concentrado em 30 cabras Saanen durante a fase inicial de lactação. A alimentação baseou-se na relação 60% de volumoso (feno de alfafa) e 40% de concentrado comercial com dois níveis de extrato etéreo (3% e 5%). As variáveis estudadas foram a quantificação e qualificação do perfil de ácidos graxos do leite total e frente ao tempo de lactação. O acréscimo de óleo de arroz na dieta diminuiu (P<0,05) a concentração de ácidos graxos saturados e aumentou (P<0,05) a concentração de ácidos graxos poli-insaturados no leite caprino. O ácido linoleico conjugado (ALC) também é afetado positivamente pelo acréscimo de óleo de arroz na dieta. A inclusão de óleo de arroz nos níveis de 3% e 5% da matéria seca, na dieta de cabras leiteiras da raça Saanen, influencia o perfil de ácidos graxos do leite, com ênfase no ALC, até a 11ª semana de lactação.(AU)


The effects of two levels of ether extract based on rice oil concentrate in 30 Saanen goats during early lactation were evaluated. The feeding was based on the ratio of 60% forage (alfalfa hay) and 40% commercial concentrate with two levels of lipids (3% and 5%). The variables were the quantification and qualification of the fatty acid profile of whole milk and forward to the time of lactation. The addition of rice bran oil in the diet decreased significantly (P<0.05) the concentration of saturated fatty acids and increased (P<0.05) the concentration of polyunsaturated fatty acids in goats' milk. The linoleic acid was also positively affected by the addition of rice bran oil in the diet. The inclusion of rice bran oil at levels of 3% and 5% of dry matter in the diet of Saanen dairy goats influences the fatty acid profile of milk, with emphasis on linoleic acid until the 11th week of lactation.(AU)


Assuntos
Animais , Cabras , Ácidos Graxos , Dieta , Leite , Lipídeos/análise , Lactação/metabolismo
4.
Arq. Inst. Biol. (Online) ; 78(1): 97-102, jan-mar, 2011.
Artigo em Português | LILACS, VETINDEX | ID: biblio-1396335

Resumo

O rebanho de ovinos no Brasil está estimado em mais de 16 milhões de cabeças. Embora o consumo da carne desta espécie ainda seja pequeno, comparado ao de outros países, o consumo de carne, inclusive ovina, tem sido associado às doenças transmitidas por alimentos, em especial a salmonelose. No presente estudo, investigou-se a ocorrência de salmonelas em linfonodos mesentéricos e conteúdo intestinal de 175 ovinos ao abate. "Pools" constituído por cinco amostras de contéudo fecal ou 5 amostras de linfonodos de 25 g foram pre-enriquecidos em 250 mL de água peptonada tamponada e incubados a 37° C por 18-24 horas. Uma alíquota de 0,1 mL do préenriquecimento foi transferida para 9,9 mL de caldo de enriquecimento Rappaport-Vassiliadis e 1,0 mL do pré-enriquecimento foi transferido para 10 mL de caldo tetrationato Muller-Kaufmann, incubados a 42° C for 24h. 10 µL do caldo de enriquecimento foi semeado superfície de placas de ágar BPLS e ágar XLT4 incubadas a 37º C for 24-48h. Colônias suspeitas de salmonela foram testadas por provas bioquímicas e serologicas. Os testes bioquímicos utilizados para identificação de Salmonella foram TSI (triple sugar iron àgar), LIA (lysine iron àgar) e ágar ureia. Sorotipagem foi realizada no Laboratório de Enterobactérias do Instituto Osvaldo Cruz. Isolou-se Salmonella Tiphymurium de um pool de linfonodos mesentéricos, provenientes de cinco animais. O fato de se observar a ocorrência de salmonela em ovino portador sadio alerta para necessidade de monitorar este micro-organismo também nesta espécie, especialmente quando destinada ao abate, com vistas à produção de alimentos seguros.


The ovine flock in Brazil is estimated at over 16 million head. Despite that meat consumption of this species is still small when compared to other countries, general meat consumption, including mutton, has been associated to food borne diseases, especially salmonellosis. In the present study, the occurrence of salmonella in mesenteric lymph nodes and intestinal content of 175 ovines during slaughter was investigated. A pool of 5 feces samples or 5 lymph node samples of 25 grams was pre-enriched in 250 mL of buffered peptone water at 37° C for 18-24h. Following this, 0.1 mL of pre-enriched broth was transferred to 9.9 mL of Rappaport-Vassiliadis enrichment broth and 1.0 mL of pre-enriched broth was transferred to 10 mL of Muller-Kaufmann tetrationate broth, incubated at 42° C for 24h. Then, a 10 µL of the enrichment broth was spread on the surface of a BPLS and an XLT4 plate, both incubated at 37º C for 24-48h. Suspected Salmonella colonies were picked from the agar and tested with biochemical and serological methods. Biochemical testing was carried out for the identification of Salmonella, using the TSI (triple sugar iron agar), LIA (lysine iron agar) and urea agar tests. Serotyping was done at the Laboratory of Enterobactérias of the Instituto Osvaldo Cruz. Salmonella Tiphymurium was isolated from a pool of mesenteric lymph nodes from 5 animals. That Salmonella was observed in healthy carrier ovines points out the necessity of monitoring this microorganism in this species as well, especially when animals are destined to slaughter, so to assure safe food production.


Assuntos
Animais , Salmonelose Animal/diagnóstico , Salmonella typhimurium/isolamento & purificação , Ovinos/microbiologia , Linfonodos/microbiologia , Abate de Animais
5.
Arq. Inst. Biol. ; 78(1)2011.
Artigo em Português | VETINDEX | ID: vti-759496

Resumo

ABSTRACT The ovine flock in Brazil is estimated at over 16 million head. Despite that meat consumption of this species is still small when compared to other countries, general meat consumption, including mutton, has been associated to food borne diseases, especially salmonellosis. In the present study, the occurrence of salmonella in mesenteric lymph nodes and intestinal content of 175 ovines during slaughter was investigated. A pool of 5 feces samples or 5 lymph node samples of 25 grams was pre-enriched in 250 mL of buffered peptone water at 37° C for 18-24h. Following this, 0.1 mL of pre-enriched broth was transferred to 9.9 mL of Rappaport-Vassiliadis enrichment broth and 1.0 mL of pre-enriched broth was transferred to 10 mL of Muller-Kaufmann tetrationate broth, incubated at 42° C for 24h. Then, a 10 L of the enrichment broth was spread on the surface of a BPLS and an XLT4 plate, both incubated at 37º C for 24-48h. Suspected Salmonella colonies were picked from the agar and tested with biochemical and serological methods. Biochemical testing was carried out for the identification of Salmonella, using the TSI (triple sugar iron agar), LIA (lysine iron agar) and urea agar tests. Serotyping was done at the Laboratory of Enterobactérias of the Instituto Osvaldo Cruz. Salmonella Tiphymurium was isolated from a pool of mesenteric lymph nodes from 5 animals. That Salmonella was observed in healthy carrier ovines points out the necessity of monitoring this microorganism in this species as well, especially when animals are destined to slaughter, so to assure safe food production.


RESUMO O rebanho de ovinos no Brasil está estimado em mais de 16 milhões de cabeças. Embora o consumo da carne desta espécie ainda seja pequeno, comparado ao de outros países, o consumo de carne, inclusive ovina, tem sido associado às doenças transmitidas por alimentos, em especial a salmonelose. No presente estudo, investigou-se a ocorrência de salmonelas em linfonodos mesentéricos e conteúdo intestinal de 175 ovinos ao abate. Pools constituído por cinco amostras de contéudo fecal ou 5 amostras de linfonodos de 25 g foram pre-enriquecidos em 250 mL de água peptonada tamponada e incubados a 37° C por 18-24 horas. Uma alíquota de 0,1 mL do pré-enriquecimento foi transferida para 9,9 mL de caldo de enriquecimento Rappaport-Vassiliadis e 1,0 mL do pré-enriquecimento foi transferido para 10 mL de caldo tetrationato Muller-Kaufmann, incubados a 42° C for 24h. 10 L do caldo de enriquecimento foi semeado superfície de placas de ágar BPLS e ágar XLT4 incubadas a 37º C for 24-48h. Colônias suspeitas de salmonela foram testadas por provas bioquímicas e serologicas. Os testes bioquímicos utilizados para identificação de Salmonella foram TSI (triple sugar iron àgar), LIA (lysine iron àgar) e ágar ureia. Sorotipagem foi realizada no Laboratório de Enterobactérias do Instituto Osvaldo Cruz. Isolou-se Salmonella Tiphymurium de um pool de linfonodos mesentéricos, provenientes de cinco animais. O fato de se observar a ocorrência de salmonela em ovino portador sadio alerta para necessidade de monitorar este micro-organismo também nesta espécie, especialmente quando destinada ao abate, com vistas à produção de alimentos seguros.

6.
Arq. bras. med. vet. zootec ; 60(3): 762-765, jun. 2008. tab
Artigo em Português | VETINDEX | ID: vti-6774

Resumo

The antimicrobial resistance of 96 Escherichia coli strains isolated from a stabilization pond system on a pig-breeding farm was evaluated. Strains were tested for their resistance against 14 antimicrobial using the agar diffusion method. E. coli strains showed resistance to tetracycline (82.3 percent), nalidixic acid (64 percent), ampicilin (41 percent), sulfamethoxazole/trimethoprin (36 percent), sulfonamide (34 percent), cloranphenicol (274 percent), ciprofloxacin (19 percent), cefaclor (16 percent), streptomicyn (7.3 percent), neomicyn (1 percent), amoxacilin/ clavulanic acid (1 percent), and amikacin (1 percent). No resistance was observed to gentamicin and tobramycin, and 37.5 percent of E. coli strains were resistant to four or more antimicrobials. The multiresistance pattern was found in strains isolated during all sampled period. Strains showed a high variability in the antimicrobial resistance pattern.(AU)


Assuntos
Animais , Resistência a Medicamentos , Suínos , Escherichia coli/isolamento & purificação , Resistência Microbiana a Medicamentos , Fezes/microbiologia
7.
Arq. bras. med. vet. zootec ; 56(4): 438-440, ago. 2004.
Artigo em Português | VETINDEX | ID: vti-2325

Resumo

Relata-se a ocorrência de artrogripose congênita em um caprino fêmea da raça Anglo-Nubiana, no município de Viamão, RS, provavelmente induzida por hipertemia ocorrida durante a gestação.(AU)


This is a report of a case of arthrogryposis in an Anglo-Nubian female kid in Rio Grande do Sul, Brazil. This pathology was probably induced by an episode of hyperthermia during gestation.(AU)


Assuntos
Animais , Feminino , Artrogripose , Cabras
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA