Explore the antiproliferative phytocompounds from ethanolic extracts of Citrus paradisi against liver cancer cell line by chemical analysis using TLC and FT-IR spectroscopy

Abstract The aim of the present study was to evaluate the in vitro antiproliferative activity of ethanolic extract of leaves and fruits Citrus paradisi plant on HepG-2 liver cell lines by MTT (3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-terazolium bromide) assay and to isolate and characterize the antiproliferative compounds by TLC (Thin layer chromatography) and FT-IR (Fourier transforms Infrared) spectroscopy. Qualitative phytochemical screening tests were performed to detect phytochemicals compounds from the crude extracts. Antioxidant activity of the plant extracts were characterized by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radical scavenging method. The results showed that antioxidant activity using DPPH were found to be increased in a concentration dependent manner and decreased cell viability and cell growth inhibition in a dose dependent manner. The findings from this study indicated that fruit extract exhibited good antiproliferation and antioxidant potential. The seven functional groups of phytocompounds such as carboxylic acid, amine salt, aromatic compounds, cyclic alkene, aldehyde, fluoro compounds and alkene were detected by FT-IR which indicated that fruit extracts of Citrus paradisi possessed vast potential as a medicinal drug especially in liver cancer treatment.

Resumo O objetivo do presente estudo foi avaliar a atividade antiproliferativa in vitro do extrato etanólico de folhas e frutos da planta Citrus paradisi em linhagens de células hepáticas HepG-2 por MTT (3- (4, 5-dimetil-2-tiazolil) -2, Ensaio de brometo de 5-difenil-2H-terazólio) e isolar e caracterizar os compostos antiproliferativos por espectroscopia de TLC (cromatografia de camada fina) e FT-IR (infravermelho com transformadas de Fourier). Testes qualitativos de triagem fitoquímica foram realizados para detectar compostos fitoquímicos nos extratos brutos. A atividade antioxidante dos extratos vegetais foi caracterizada pelo método de eliminação de radicais livres DPPH (2,2-difenil-1-picrilhidrazil). Os resultados mostraram que a atividade antioxidante usando DPPH aumentou de uma maneira dependente da concentração e diminuiu a viabilidade celular e a inibição do crescimento celular de uma maneira dependente da dose. Os resultados deste estudo indicaram que o extrato de fruta exibiu bom potencial antiproliferação e antioxidante. Os sete grupos funcionais de fitocompostos, como ácido carboxílico, sal de amina, compostos aromáticos, alceno cíclico, aldeído, compostos de flúor e alceno, foram detectados por FT-IR, o que indicou que extratos de frutas de Citrus paradisi possuíam vasto potencial como medicamento, especialmente no tratamento de câncer do fígado.

Explore the antiproliferative phytocompounds from ethanolic extracts of Citrus paradisi against liver cancer cell line by chemical analysis using TLC and FT-IR spectroscopy / Explorar os fitocompostos antiproliferativos de extratos etanólicos de Citrus paradisi contra linhagem de células de câncer de fígado por análise química usando espectroscopia de TLC e FT-IR

The aim of the present study was to evaluate the in vitro antiproliferative activity of ethanolic extract of leaves and fruits Citrus paradisi plant on HepG-2 liver cell lines by MTT (3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2Hterazolium bromide) assay and to isolate and characterize the antiproliferative compounds by TLC (Thin layer chromatography) and FT-IR (Fourier transforms Infrared) spectroscopy. Qualitative phytochemical screening tests were performed to detect phytochemicals compounds from the crude extracts. Antioxidant activity of the plant extracts were characterized by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radical scavenging method. The results showed that antioxidant activity using DPPH were found to be increased in a concentration dependent manner and decreased cell viability and cell growth inhibition in a dose dependent manner. The findings from this study indicated that fruit extract exhibited good antiproliferation and antioxidant potential. The seven functional groups of phytocompounds such as carboxylic acid, amine salt, aromatic compounds, cyclic alkene, aldehyde, fluoro compounds and alkene were detected by FT-IR which indicated that fruit extracts of Citrus paradisi possessed vast potential as a medicinal drug especially in liver cancer treatment.

O objetivo do presente estudo foi avaliar a atividade antiproliferativa in vitro do extrato etanólico de folhas e frutos da planta Citrus paradisi em linhagens de células hepáticas HepG-2 por MTT (3- (4, 5-dimetil-2-tiazolil) -2, Ensaio de brometo de 5-difenil-2H-terazólio) e isolar e caracterizar os compostos antiproliferativos por espectroscopia de TLC (cromatografia de camada fina) e FT-IR (infravermelho com transformadas de Fourier). Testes qualitativos de triagem fitoquímica foram realizados para detectar compostos fitoquímicos nos extratos brutos. A atividade antioxidante dos extratos vegetais foi caracterizada pelo método de eliminação de radicais livres DPPH (2,2-difenil-1-picrilhidrazil). Os resultados mostraram que a atividade antioxidante usando DPPH aumentou de uma maneira dependente da concentração e diminuiu a viabilidade celular e a inibição do crescimento celular de uma maneira dependente da dose. Os resultados deste estudo indicaram que o extrato de fruta exibiu bom potencial antiproliferação e antioxidante. Os sete grupos funcionais de fitocompostos, como ácido carboxílico, sal de amina, compostos aromáticos, alceno cíclico, aldeído, compostos de flúor e alceno, foram detectados por FT-IR, o que indicou que extratos de frutas de Citrus paradisi possuíam vasto potencial como medicamento, especialmente no tratamento de câncer do fígado.

Effects of Dietary Supplementations of Synbiotics on Growth Performance, Carcass Characteristics and Nutrient Digestibility of Broiler Chicken

ABSTRACT The study aimed to explore the effect of dietary supplementation of synbiotics on growth performance, carcass characteristics and nutrient digestibility in broiler chicken. For this purpose, three hundred 1-day-old Cobb-500 broiler chicks were purchased from the hatchery and randomly distributed into five dietary treatment groups. Each treatment had six replicates, each containing 10 chicks. The experimental diets were supplemented with 0, 700, 1200, 1700 or 2200 g/ton of feed synbiotics and respectively designated as A, B, C, D and E, with A being the control diet. Feed intake, weight gain and feed conversion ratio data were recorded on a weekly basis. At the research trials end, two birds from each pen were randomly selected and slaughtered to get data on carcass characteristics. Results showed that group Cs feed intake was reduced (p 0.05) when compared to control. Body weight was similar (p>0.05) among all treatments. However, feed conversion ratio was significantly improved (p 0.05) in group C as compared to other dietary treatments. Nutrient digestibility was improved (p 0.05) in group B and C, as compared to control. Carcass characteristics were not significantly affected and remained the same across all treatments. However, liver weight decreased in birds fed diet C. Meat quality and antibody titer were not affected in any of the dietary treatments. It is concluded that synbiotics can be safely used up to 1200 g/ton of feed, improving bird performance without harmful effects on bird health.

Effect of Exogenous Emulsifier (Lyso-Phospholipid) Supplementation in the Broiler Diet, on the Feed Intake and Growth Performance During Grower Phase

The objective of this experiment was to investigate the effect of supplementation of an exogenous emulsifier (lyso-phospholipid) in the diet of growing broilers on growth performance and digestibility. A total of 1224 Ross-308 ten day old broiler chicks were distributed into two experimental treatments in such a way that each treatment had twelve replicates with fifty-one birds per replicate. Two experimental diets were formulated with and without emulsifier supplementation according to the nutrition standards of Ross 308. Feed intake and body weight gain of the broilers were measured on a daily basis and feed conversion ratio was also calculated. Nutrient digestibility was determined on the 25th day of age. Analysis of variance under completely randomized design technique was used to analyze the data. Feed intake was increased (p 0.05) by supplementation of emulsifier in the broiler diet on theat 12th, 13th, 21th, 22th, 23th, 24th, and 25th days. Bodyweight gain was not affected (p>0.05) with or without emulsifier supplementation in the broiler diet during 11-25 days of life. However, feed conversion ratio was effected (p 0.05) by emulsifier supplementation and increased from days 21-25th. Nutrient digestibility (dry matter, fat, and crude protein) in the grower phase was decreased (p 0.05) by supplementation of emulsifier in the diet. It can be concluded that supplementation of an exogenous emulsifier in the diet did not show positive effect on the growth performance during the grower phase of broilers, while nutrient digestibility showed adverse effect. Emulsifier supplementation should be tested after 25 days of the life of broilers.(AU)

Effect of Exogenous Emulsifier (Lyso-Phospholipid) Supplementation in the Broiler Diet, on the Feed Intake and Growth Performance During Grower Phase

The objective of this experiment was to investigate the effect of supplementation of an exogenous emulsifier (lyso-phospholipid) in the diet of growing broilers on growth performance and digestibility. A total of 1224 Ross-308 ten day old broiler chicks were distributed into two experimental treatments in such a way that each treatment had twelve replicates with fifty-one birds per replicate. Two experimental diets were formulated with and without emulsifier supplementation according to the nutrition standards of Ross 308. Feed intake and body weight gain of the broilers were measured on a daily basis and feed conversion ratio was also calculated. Nutrient digestibility was determined on the 25th day of age. Analysis of variance under completely randomized design technique was used to analyze the data. Feed intake was increased (p 0.05) by supplementation of emulsifier in the broiler diet on theat 12th, 13th, 21th, 22th, 23th, 24th, and 25th days. Bodyweight gain was not affected (p>0.05) with or without emulsifier supplementation in the broiler diet during 11-25 days of life. However, feed conversion ratio was effected (p 0.05) by emulsifier supplementation and increased from days 21-25th. Nutrient digestibility (dry matter, fat, and crude protein) in the grower phase was decreased (p 0.05) by supplementation of emulsifier in the diet. It can be concluded that supplementation of an exogenous emulsifier in the diet did not show positive effect on the growth performance during the grower phase of broilers, while nutrient digestibility showed adverse effect. Emulsifier supplementation should be tested after 25 days of the life of broilers.

Emulsifier Supplementation Response in Ross 308 Broilers at 1-10 Days

The study was conducted to check the effect of emulsifier supplementation on growth performance of broiler birds on starter phase, from days 1 to 10. In the current study, a total of twelve hundred twenty-four Ross 308 broiler one day old chicks were used. Ross 308 broiler chicks were divided into two groups in such a way that each group had12 replicates, and each replicate had 51 birds. The duration of the current trial was of 10 days. Chicks were fed experimental diets with or without emulsifier supplementation. Daily feed intake, body weight, feed conversion ratio and nutrient digestibility were recorded. Mortality was recorded on a daily basis, and feed intake was also corrected for mortality. Data collected from the experiment was interpreted by using analysis of variance method using SPSSTM under Completely Randomized Design. Results showed that emulsifier supplementation improved (p 0.05) feed intake in broiler birds on the 2nd, 6th, 7th and 9th­ days of the starter phase. Furthermore, emulsifier supplementation (p 0.05) affected the body weight on the 3rd and 10th day of the trial. Emulsifier supplementation also affected (p 0.05) feed conversion ratio on the 10th day of the trial. However, nutrient digestibility was not affected by the supplementation of emulsifier in the diet of broiler chicks. Results showed that emulsifier supplementation should be started on day 10.(AU)

Emulsifier Supplementation Response in Ross 308 Broilers at 1-10 Days

The study was conducted to check the effect of emulsifier supplementation on growth performance of broiler birds on starter phase, from days 1 to 10. In the current study, a total of twelve hundred twenty-four Ross 308 broiler one day old chicks were used. Ross 308 broiler chicks were divided into two groups in such a way that each group had12 replicates, and each replicate had 51 birds. The duration of the current trial was of 10 days. Chicks were fed experimental diets with or without emulsifier supplementation. Daily feed intake, body weight, feed conversion ratio and nutrient digestibility were recorded. Mortality was recorded on a daily basis, and feed intake was also corrected for mortality. Data collected from the experiment was interpreted by using analysis of variance method using SPSSTM under Completely Randomized Design. Results showed that emulsifier supplementation improved (p 0.05) feed intake in broiler birds on the 2nd, 6th, 7th and 9th­ days of the starter phase. Furthermore, emulsifier supplementation (p 0.05) affected the body weight on the 3rd and 10th day of the trial. Emulsifier supplementation also affected (p 0.05) feed conversion ratio on the 10th day of the trial. However, nutrient digestibility was not affected by the supplementation of emulsifier in the diet of broiler chicks. Results showed that emulsifier supplementation should be started on day 10.