Correlation of sperm parameters with fertility in two commercial pig lines

Background: One of the most important parameters to be considered in the assessment of male fertility or in the evaluation of methods to preserve sperm cells is the study of sperm viability. Considering that semen quality is of great importance for artificial insemination AI) in sows and for the production of an optimal number of piglets, complementary test results can be correlated with the number of piglets born after AI. The present study aimed to characterise the seminal pattern of breeding boars from two commercial lines and to correlate this pattern with the prolificacy of sows.Materials, Methods & Results: The reproductive performance of four boars from two commercial lines (A and B) was determined by evaluating physical and morphological semen parameters and by conducting a complementary analysis of semen. Semen was subjected to the following parameters: total sperm motility, sperm vigor, major sperm defects, minor sperm defects, and total sperm defects, in addition to complementary tests (supravital staining and hypoosmotic swelling tests). The semen was evaluated immediately after collection and every 12 h for up to 72 h of cold storage. A total of 163 breeding sows were inseminated. To evaluate sow prolificacy, the total number of piglets born was recorded. In general, the values obtained for the tests assessing sperm viability (particularly sperm motility and...(AU)

Correlation of sperm parameters with fertility in two commercial pig lines

Background: One of the most important parameters to be considered in the assessment of male fertility or in the evaluation of methods to preserve sperm cells is the study of sperm viability. Considering that semen quality is of great importance for artificial insemination AI) in sows and for the production of an optimal number of piglets, complementary test results can be correlated with the number of piglets born after AI. The present study aimed to characterise the seminal pattern of breeding boars from two commercial lines and to correlate this pattern with the prolificacy of sows.Materials, Methods & Results: The reproductive performance of four boars from two commercial lines (A and B) was determined by evaluating physical and morphological semen parameters and by conducting a complementary analysis of semen. Semen was subjected to the following parameters: total sperm motility, sperm vigor, major sperm defects, minor sperm defects, and total sperm defects, in addition to complementary tests (supravital staining and hypoosmotic swelling tests). The semen was evaluated immediately after collection and every 12 h for up to 72 h of cold storage. A total of 163 breeding sows were inseminated. To evaluate sow prolificacy, the total number of piglets born was recorded. In general, the values obtained for the tests assessing sperm viability (particularly sperm motility and...

Viabilidade espermática do sêmen congelado de suínos da raça Piau avaliada pelo teste de termorresistência / Spermatic viability of cryopreserved semen of Piau swine breed analyzed by thermo resistant test

O objetivo do presente trabalho foi verificar o efeito de três protocolos de criopreservação de sêmen sobre a viabilidade espermática pós-descongelamento de suínos da raça Piau, avaliados por meio do teste de termorresistência (TTR). Para o congelamento, os ejaculados foram fracionados e submetidos aos seguintes tratamentos: protocolo 1 -preconizado por Fürst et al. (2005), modificado quanto aos meios diluentes; protocolo 2 -preconizado por Fürst et al. (2005), modificado quanto à curva de resfriamento; e protocolo 3 -preconizado por Ohata et al. (2001). Após o descongelamento o sêmen foi submetido ao teste de termo-resistência (38 °C por 2 horas), procedendo-se avaliações de motilidade e vigor espermáticos a cada 30 minutos. As médias registradas para motilidade imediatamente após o descongelamento foram de 20,9±12,4; 29,5±10,9 e 49,5±12,1%; respectivamente para os protocolos 1, 2 e 3. Os resultados do TTR evidenciam queda gradual dos parâmetros de MOT ao longo de 2 horas de duração nos três protocolos utilizados. Observou-se que os valores médios de motilidade espermática verificados no protocolo 3 foram maiores que as registradas nos outros dois protocolos, em todos os períodos avaliados. Do mesmo modo, a porcentagem média de espermatozoides móveis obtidos no protocolo 2 foi maior que a obtida no protocolo 1 nos primeiros 30 minutos de avaliação, sendo que após este período não houve diferença entre eles. Com relação ao vigor espermático entre os protocolos, verificou-se que o protocolo 3 apresentou resultados melhores em relação aos demais. As melhores médias para o protocolo 3, demonstram a superioridade do mesmo na criopreservação das células espermáticas dos animais do presente estudo.

The objective of this study was to verify three protocols of semen cryopreservation on spermatic viability after thawing from Piau swine breed, by thermo resistant test (TRT). To freezing, the ejaculates was split and submitted to three protocols: Protocol 1 -proposed by Furst et al. (2005), altered by diluent media; Protocol 2 -proposed by Furst et al. (2005), altered by cooled curve; and Protocol 3 -proposed by Ohata et al. (2001). After thawing, semen was submitted to TRT (37°C during 2 hours), and sperm motility was analyzed at 30 minutes of interval. Motility after thawing was 20.9±12.4, 29.5±10.9 and 49.5±12.1%, respectively to P1, P2 and P3. The TRT results show gradually decrease of motility and vigor along 2 hours of test procedure utilized. It was observed that the mean values of sperm motility observed in protocol 3 were higher than those recorded in the other two protocols in all periods. Similarly, the mean percentage of motile spermatozoa obtained in protocol 2 was higher than that obtained in protocol 1 in the first 30 minutes of evaluation, and after this period there was no difference between them. Regarding the sperm vigor between protocols, it was found that the protocol 3 showed better results than the other. The protocol 3 tested by Piau boars show highest values in cellular semen cryopreservation.

Viabilidade espermática do sêmen congelado de suínos da raça Piau avaliada pelo teste de termorresistência / Spermatic viability of cryopreserved semen of Piau swine breed analyzed by thermo resistant test

O objetivo do presente trabalho foi verificar o efeito de três protocolos de criopreservação de sêmen sobre a viabilidade espermática pós-descongelamento de suínos da raça Piau, avaliados por meio do teste de termorresistência (TTR). Para o congelamento, os ejaculados foram fracionados e submetidos aos seguintes tratamentos: protocolo 1 -preconizado por Fürst et al. (2005), modificado quanto aos meios diluentes; protocolo 2 -preconizado por Fürst et al. (2005), modificado quanto à curva de resfriamento; e protocolo 3 -preconizado por Ohata et al. (2001). Após o descongelamento o sêmen foi submetido ao teste de termo-resistência (38 °C por 2 horas), procedendo-se avaliações de motilidade e vigor espermáticos a cada 30 minutos. As médias registradas para motilidade imediatamente após o descongelamento foram de 20,9±12,4; 29,5±10,9 e 49,5±12,1%; respectivamente para os protocolos 1, 2 e 3. Os resultados do TTR evidenciam queda gradual dos parâmetros de MOT ao longo de 2 horas de duração nos três protocolos utilizados. Observou-se que os valores médios de motilidade espermática verificados no protocolo 3 foram maiores que as registradas nos outros dois protocolos, em todos os períodos avaliados. Do mesmo modo, a porcentagem média de espermatozoides móveis obtidos no protocolo 2 foi maior que a obtida no protocolo 1 nos primeiros 30 minutos de avaliação, sendo que após este período não houve diferença entre eles. Com relação ao vigor espermático entre os protocolos, verificou-se que o protocolo 3 apresentou resultados melhores em relação aos demais. As melhores médias para o protocolo 3, demonstram a superioridade do mesmo na criopreservação das células espermáticas dos animais do presente estudo.(AU)

The objective of this study was to verify three protocols of semen cryopreservation on spermatic viability after thawing from Piau swine breed, by thermo resistant test (TRT). To freezing, the ejaculates was split and submitted to three protocols: Protocol 1 -proposed by Furst et al. (2005), altered by diluent media; Protocol 2 -proposed by Furst et al. (2005), altered by cooled curve; and Protocol 3 -proposed by Ohata et al. (2001). After thawing, semen was submitted to TRT (37°C during 2 hours), and sperm motility was analyzed at 30 minutes of interval. Motility after thawing was 20.9±12.4, 29.5±10.9 and 49.5±12.1%, respectively to P1, P2 and P3. The TRT results show gradually decrease of motility and vigor along 2 hours of test procedure utilized. It was observed that the mean values of sperm motility observed in protocol 3 were higher than those recorded in the other two protocols in all periods. Similarly, the mean percentage of motile spermatozoa obtained in protocol 2 was higher than that obtained in protocol 1 in the first 30 minutes of evaluation, and after this period there was no difference between them. Regarding the sperm vigor between protocols, it was found that the protocol 3 showed better results than the other. The protocol 3 tested by Piau boars show highest values in cellular semen cryopreservation.(AU)