Electrochemotherapy associated with calcium electroporation in metastatic feline cutaneous malignant melanoma

Background: Calcium electroporation (CaEP) is a novel therapeutic treatment that has been studied for cancer due to itsselective killing cancer cells by necrosis and danger signals. Besides that, electrochemotherapy (ECT) is an effective local treatment that involves the administration of chemotherapeutic drugs followed by delivery of electrical pulses to thetumor. The combination with ECT and CaEP has been reported in literature suggesting that additional response of immunesystem could have been enhanced by electroporation with calcium. This case, report on the successful treatment with CaEPcombined with ECT for treatment of a regional metastasis in a feline model of malignant melanoma.Case: A 9-year-old, mixed breed cat was referred to the veterinary clinic with a 2-month history of cutaneous peripalpebralplaque lesion (0.19 cm³) and a submandibular lymph node enlargement (0.5 cm³). Incisional biopsy of the cutaneous lesionand fine-needle aspiration of submandibular lymph node confirmed a cutaneous melanoma with submandibular lymph nodemetastasis. Tumor staging was set in T1N1M0 according to WHO staging criteria. ECT for the primary lesion and lymphnode metastasis was proposed. For the ECT, bleomycin (15,000 UI/m²) application was performed intravenous followedby electroporation (8 pulses of 100 μs at 1000 V/cm, and 1 Hz) using a needle array electrode consisted of two parallelrows with six needles in each row. At 28-day post-ECT complete remission of the primary tumor and metastatic foci wasachieved. However, 120 days after ECT, recurrence was observed in submandibular and retropharyngeal...

Electrochemotherapy associated with calcium electroporation in metastatic feline cutaneous malignant melanoma

Background: Calcium electroporation (CaEP) is a novel therapeutic treatment that has been studied for cancer due to itsselective killing cancer cells by necrosis and danger signals. Besides that, electrochemotherapy (ECT) is an effective local treatment that involves the administration of chemotherapeutic drugs followed by delivery of electrical pulses to thetumor. The combination with ECT and CaEP has been reported in literature suggesting that additional response of immunesystem could have been enhanced by electroporation with calcium. This case, report on the successful treatment with CaEPcombined with ECT for treatment of a regional metastasis in a feline model of malignant melanoma.Case: A 9-year-old, mixed breed cat was referred to the veterinary clinic with a 2-month history of cutaneous peripalpebralplaque lesion (0.19 cm³) and a submandibular lymph node enlargement (0.5 cm³). Incisional biopsy of the cutaneous lesionand fine-needle aspiration of submandibular lymph node confirmed a cutaneous melanoma with submandibular lymph nodemetastasis. Tumor staging was set in T1N1M0 according to WHO staging criteria. ECT for the primary lesion and lymphnode metastasis was proposed. For the ECT, bleomycin (15,000 UI/m²) application was performed intravenous followedby electroporation (8 pulses of 100 μs at 1000 V/cm, and 1 Hz) using a needle array electrode consisted of two parallelrows with six needles in each row. At 28-day post-ECT complete remission of the primary tumor and metastatic foci wasachieved. However, 120 days after ECT, recurrence was observed in submandibular and retropharyngeal...(AU)

Avaliação do perfil clínico, laboratorial e molecular de cães suspeitos de Erlichiose e tratados com doxicicllina

A erliquiose canina é uma doença endêmica, transmitida pelo artrópode Riphicephalus sanguineus, presente em quase todas as regiões do Brasil caracterizadas por ser multissistêmica, apresentando três fases: aguda, subclínica e crônica. Devido à importância e alta incidência da doença, objetivou-se com esse trabalho avaliar a eficiência e evolução do tratamento usual com 10 mg/Kg de doxiciclina, a cada 12 horas, durante três semanas. Foram utilizados 15 cães, da rotina, SRD, com idade média de 3,4 ± 1,5 anos, de ambos os sexos. Realizou-se o exame físico geral, pesquisa de hemoparasitas, sorologia e hemograma no primeiro dia, 14° e 21° do tratamento. No primeiro dia os animais apresentaram: hipertemia (40%), linfadenomegalia (100%), apatia (66,6%), mucosas hipocoradas (66,7%), trombocitopenia (100%), anemia (80%), leucocitose (33,3%), linfopenia (6,6%), eosinofilia (46,6%), linfocitose (46,6) e sorologia positiva para E.canis (100%). A hiperglobulinemia foi verificada em 57,1%, 69,2%, 81,2% e 28,6% dos animais, nos momentos -7, 0, +14 e +21dias de tratamento, respectivamente. O quadro de hipoalbuminemia foi detectado em 42,9%, 53,8%, 37,5%, 28,6% nos momentos -7, 0, +14 e +21dias de tratamento, respectivamente. Ao se avaliar os parâmetros bioquímicos renais e hepáticos dos cães infectados com E. canis não foram observadas alterações significativas. A PCR mostrou-se positiva para E. canis em 3 animais no pré tratamento. Um animal apresentou-se positivo durante e no fim do tratamento, indicando persistência da infecção e a necessidade de um novo ciclo de tratamento. Dois animais se apresentaram positivos na PCR para Anaplasma platys. Embora tenha ocorrido uma melhora significativa no estado geral dos cães, observou-se que o quadro hematológico ainda não havia estabelecido sua plenitude em todos os animais