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1.
Arq. bras. med. vet. zootec ; 68(5): 1390-1394, set.-out. 2016. tab
Artigo em Português | LILACS, VETINDEX | ID: biblio-868194

Resumo

Superovulatory response and embryo yield in 19 Morada Nova and 20 Somalis Brasileira ewes was analyzed. All animals were synchronized with the insertion of an intravaginal device (CIDR®) on Day 0, replaced by a new device on Day 7, which remained in place until Day 14 and superovulated with 133mg of porcine FSH (pFSH) in decreasing doses at 12h intervals from Day 12 until Day 15 of the treatment, and a single dose of equine chorionic gonadotropin (eCG, 200UI) on Day 14 (i.e., administered in CIDR removal). Fifty hours after CIDR® removal, females were inseminated by laparoscopy. All embryos were recovered by laparotomy 5 days after insemination. Sheep which responded to the superovulation protocol (P>0.05) included 74% of the Morada Nova ewes and 50% of the Somalis Brasileira ewes. Morada Nova showed better results (P<0.05) than Somalis Brasileira in number of ovulations (15.38 ± 5.24 vs. 10.56 ± 2.83), total structures (11.00 ± 7.55 vs. 3.33 ± 1.94) and embryo yields (6.79 ± 5.35 vs. 2.90 ± 2.18). Despite the high fertilization rate, degenerate embryo rate was high for both breeds, with an overall rate of 39% (57/145). In conclusion, superovulatory response and embryo yields in Morada Nova ewes were considered sufficient to justify the use of this procedure in genetic resources conservation programs. However, improvements to embryo quality and control of precocious regression of corpus luteum are necessary to produce better results in the MOET program, with minimal variations and maximum embryo yield in Morada Nova and Somalis Brasileira ewes.(AU)


Assuntos
Animais , Embrião de Mamíferos , Hormônio Foliculoestimulante/análise , Ovinos/embriologia , Superovulação , Variação Genética
2.
Arq. bras. med. vet. zootec. (Online) ; 68(5): 1390-1394, set.-out. 2016. tab
Artigo em Português | VETINDEX | ID: vti-16319

Resumo

Superovulatory response and embryo yield in 19 Morada Nova and 20 Somalis Brasileira ewes was analyzed. All animals were synchronized with the insertion of an intravaginal device (CIDR®) on Day 0, replaced by a new device on Day 7, which remained in place until Day 14 and superovulated with 133mg of porcine FSH (pFSH) in decreasing doses at 12h intervals from Day 12 until Day 15 of the treatment, and a single dose of equine chorionic gonadotropin (eCG, 200UI) on Day 14 (i.e., administered in CIDR removal). Fifty hours after CIDR® removal, females were inseminated by laparoscopy. All embryos were recovered by laparotomy 5 days after insemination. Sheep which responded to the superovulation protocol (P>0.05) included 74% of the Morada Nova ewes and 50% of the Somalis Brasileira ewes. Morada Nova showed better results (P<0.05) than Somalis Brasileira in number of ovulations (15.38 ± 5.24 vs. 10.56 ± 2.83), total structures (11.00 ± 7.55 vs. 3.33 ± 1.94) and embryo yields (6.79 ± 5.35 vs. 2.90 ± 2.18). Despite the high fertilization rate, degenerate embryo rate was high for both breeds, with an overall rate of 39% (57/145). In conclusion, superovulatory response and embryo yields in Morada Nova ewes were considered sufficient to justify the use of this procedure in genetic resources conservation programs. However, improvements to embryo quality and control of precocious regression of corpus luteum are necessary to produce better results in the MOET program, with minimal variations and maximum embryo yield in Morada Nova and Somalis Brasileira ewes.(AU)


Assuntos
Animais , Ovinos/embriologia , Embrião de Mamíferos , Superovulação , Hormônio Foliculoestimulante/análise , Variação Genética
3.
Anim. Reprod. (Online) ; 12(2): 312-315, Apr.-June.2015. tab
Artigo em Inglês | VETINDEX | ID: biblio-1461154

Resumo

This study was done to evaluate the efficiency of GnRH along with long-term and short-term synchronization protocols on ovulation induction and corpus luteum development. Ewes underwent four protocols: Long+GnRH (n = 11) with vaginal sponge (60 mg MAP) for 12 days along with 300 IU of eCG on day 12 and 0.025 mg of GnRH 27 h after sponge removal; Long (n = 10) with vaginal sponge for 12 days along with 300 IU of eCG on day 12; Short GnRH (n = 10) with vaginal sponge for 7 days along with 37.5 μg of D-cloprostenol on day 5 and 300 IU of eCG on day 7, plus 0.025 mg of GnRH used 27 h after sponge removal; and Short (n = 10) with vaginal sponge for 7 days. D-cloprostenol (37.5 μg) was administered on day 5 and eCG (300 IU) was administered on day 7.Ovulation was evaluated 52, 56, 60, 66, 72, 76 h after sponge removal. Blood was collected twelve days after sponge removal to measure progesterone concentration. On this same day, the corpus luteum was measure and counted. When GnRH was used, all ewes ovulated, while 70 and 80% of ewes ovulated in protocols that had not received GnRH (Long and Short, respectively). The GnRH accelerated ovulation (P< 0.05) in relation to sponge removal in both protocols and induced ovulation in approximately 28 h. The GnRH Was effective in inducing ovulation without decreasing the corpus luteum volume and progesterone concentration.


Assuntos
Feminino , Animais , Hormônio Liberador de Gonadotropina/análise , Hormônio Liberador de Gonadotropina/química , Inseminação Artificial/veterinária , Ovinos/anatomia & histologia , Ovinos/fisiologia , Sincronização do Estro
4.
Anim. Reprod. ; 12(2): 312-315, Apr.-June.2015. tab
Artigo em Inglês | VETINDEX | ID: vti-745446

Resumo

This study was done to evaluate the efficiency of GnRH along with long-term and short-term synchronization protocols on ovulation induction and corpus luteum development. Ewes underwent four protocols: Long+GnRH (n = 11) with vaginal sponge (60 mg MAP) for 12 days along with 300 IU of eCG on day 12 and 0.025 mg of GnRH 27 h after sponge removal; Long (n = 10) with vaginal sponge for 12 days along with 300 IU of eCG on day 12; Short GnRH (n = 10) with vaginal sponge for 7 days along with 37.5 μg of D-cloprostenol on day 5 and 300 IU of eCG on day 7, plus 0.025 mg of GnRH used 27 h after sponge removal; and Short (n = 10) with vaginal sponge for 7 days. D-cloprostenol (37.5 μg) was administered on day 5 and eCG (300 IU) was administered on day 7.Ovulation was evaluated 52, 56, 60, 66, 72, 76 h after sponge removal. Blood was collected twelve days after sponge removal to measure progesterone concentration. On this same day, the corpus luteum was measure and counted. When GnRH was used, all ewes ovulated, while 70 and 80% of ewes ovulated in protocols that had not received GnRH (Long and Short, respectively). The GnRH accelerated ovulation (P< 0.05) in relation to sponge removal in both protocols and induced ovulation in approximately 28 h. The GnRH Was effective in inducing ovulation without decreasing the corpus luteum volume and progesterone concentration.(AU)


Assuntos
Animais , Feminino , Ovinos/anatomia & histologia , Ovinos/fisiologia , Sincronização do Estro , Hormônio Liberador de Gonadotropina/análise , Hormônio Liberador de Gonadotropina/química , Inseminação Artificial/veterinária
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