Influence of glycerol addition on the quality of cat epididymal sperm during the freeze-thaw process / Influência da adição do glicerol sobre a qualidade dos espermatozoides epididimários de gato durante as etapas da congelação-descongelação

Cryopreservation of epididymal sperm is a useful tool for preserving the genetic potential of valuable animal specimens. The domestic cat is used as a model to study and develop cryogenics for other felines. However, regulation of the entire cryopreservation process is essential for the success of this biotechnology. Thus, our aim was to evaluate the effects of glycerol equilibration time and freezethaw stages on the quality of epididymal sperm obtained from domestic cats. Epididymal sperm were recovered with TRIS and immediately evaluated for total motility (TM), vigor, viability, membrane functionality (HOST), and morphology. Then, TRIS-20% egg yolk was added to the samples, which were equally divided into two 1.5 mL tubes and refrigerated at 4 ºC for 1 hour. Subsequently, glycerol was added at a final concentration of 5%. The samples were incubated with glycerol (equilibration time) for either 5 or 10 minutes (groups G5 and G10, respectively) and then frozen. Thawing occurred at 37 ºC for 30 seconds. The samples were evaluated at all stages. A reduction in TM was observed only after thawing; however, it was higher in G5 (39.00 ± 4.07%) than in G10 (18.50 ± 4.54%). Vigor declined in both groups after thawing; however, they did not differ from each other. Sperm viability was maintained in G5 after glycerolization (53.60 ± 2.59%); in G10, sperm viability decreased in the glycerolized sample (48.80 ± 2.93%) when compared to that in the fresh sample (59.90 ± 1.74%). Post-thaw viability of G5 (33.80 ± 1.89%) was higher than that of G10 (18.80 ± 3.01%). In the HOST, a decrease in viability was only observed after thawing, with no difference between the groups (41.50 ± 2.84% for G5 and 40.20 ± 3.49% for G10). With regard to sperm morphology, normal sperm decreased while sperm with post-thaw secondary defects increased in both groups. In conclusion, a shorter equilibration time for glycerolization preserves epididymal sperm quality better and the freeze-thaw process is the most critical stage of thawing.(AU)

A criopreservação dos espermatozoides epididimários é uma ferramenta útil para preservar o potencial genético de um animal valioso. Além disso, o gato doméstico é modelo eleito para o estudo e desenvolvimento da criogenia para os demais felinos. Contudo, para o sucesso dessa biotécnica é essencial o controle de todo o processo de criopreservação. Assim, objetivou-se avaliar o efeito do tempo de equilíbrio da glicerolização e das etapas da congelação-descongelação sobre a qualidade dos espermatozoides epididimários de gato doméstico. Para tanto, espermatozoides epididimários foram recuperados com TRIS e imediatamente avaliados quanto à motilidade total (MT), vigor, viabilidade, funcionalidade de membrana (HOST) e morfologia. Em seguida, as amostras foram adicionadas de TRIS-gema a 20%, fracionadas igualmente em dois tubos de 1,5 mL, refrigeradas a 4 ºC por 1 hora e, posteriormente, adicionadas de glicerol na concentração final de 5%. As amostras foram incubadas com glicerol (tempo de equilíbrio) por 5 ou 10 minutos (grupos G5 e G10, respectivamente) e depois congeladas. A descongelação ocorreu a 37 ºC por 30 segundos. As amostras foram avaliadas em todas as etapas. Uma redução na MT foi observada apenas na pós-descongelação, no entanto G5 (39,00 ± 4,07%) foi superior ao G10 (18,50 ± 4,54%). O vigor declinou pós-descongelação em ambos os grupos; contudo, não diferiram entre si. A viabilidade espermática foi mantida no G5 pós-glicerolização (53,60 ± 2,59%), diferentemente do observado em G10, em que a amostra glicerolizada (48,80 ± 2,93%) reduziu em relação à fresca (59,90 ± 1,74%). A viabilidade pós-descongelação de G5 (33,80 ± 1,89%) foi superior à de G10 (18,80 ± 3,01%). No HOST, uma redução da viabilidade só foi observada pósdescongelação, não havendo diferença entre os grupos (41,50 ± 2,84% para G5 e 40,20 ± 3,49% para G10). Em relação à morfologia espermática, os espermatozoides normais diminuíram, enquanto os espermatozoides com defeitos secundários pós-descongelação aumentaram em ambos os grupos. Conclui-se que um menor tempo de equilíbrio para a glicerolização preserva melhor a qualidade dos espermatozoides epididimários e a etapa mais crítica do processo de congelação-descongelação é a descongelação.(AU)

Diâmetro biparietal fetal por ultrassonografia como ferramenta para estimar a idade gestacional em cadelas da raça buldogue francês / Fetal biparietal diameter by ultrassonography as a tool for estimating gestational age in bitches of the French Bulldog race

O acompanhamento gestacional por ultrassonografia permite avaliar o desenvolvimento do concepto e estimar a idade gestacional (IG). No entanto, a fórmula de Nyland e Mattoon para cálculo da IG foi desenvolvida no Beagle. Cães braquicefálicos como o Buldogue Francês apresentam maior predisposição à distocia, o que torna ainda mais importante a correta estimativa da IG. Objetivou-se avaliar a fórmula de Nyland e Mattoon para datar a IG em Buldogue Francês. Cinco cadelas foram submetidas à ultrassonografia abdominal aos 55 dias pós-inseminação. Foi mensurado o diâmetro biparietal (DBP) fetal e aplicado na fórmula. Os resultados foram expressos na forma de média e erro padrão. O DBP foi de 2,14±0,02 e a IG correspondeu a 52 dias. A fórmula não tem confiabilidade para estimar a IG nesta raça.

The ultrasound gestational follow-up allows the evaluation of the development of the concept and the estimation of gestational age (GI). However, the Nyland and Mattoon formula for calculating the GI was developed in the Beagle. Brachycephalic dogs such as the French Bulldog have a greater predisposition to dystocia, which makes the correct estimation of GI even more important. The objective of this study was to evaluate the Nyland and Mattoon formula for dating GI in French Bulldog. Five bitches were submitted to abdominal ultrasound at 55 days post-insemination. The fetal biparietal diameter (DBP) was measured and applied in the formula. The results were expressed as mean and standard error. DBP was 2.14±0.02 and IG corresponded to 52 days. The formula has no reliability to estimate GI in this breed.

Diâmetro biparietal fetal por ultrassonografia como ferramenta para estimar a idade gestacional em cadelas da raça buldogue francês / Fetal biparietal diameter by ultrassonography as a tool for estimating gestational age in bitches of the French Bulldog race

O acompanhamento gestacional por ultrassonografia permite avaliar o desenvolvimento do concepto e estimar a idade gestacional (IG). No entanto, a fórmula de Nyland e Mattoon para cálculo da IG foi desenvolvida no Beagle. Cães braquicefálicos como o Buldogue Francês apresentam maior predisposição à distocia, o que torna ainda mais importante a correta estimativa da IG. Objetivou-se avaliar a fórmula de Nyland e Mattoon para datar a IG em Buldogue Francês. Cinco cadelas foram submetidas à ultrassonografia abdominal aos 55 dias pós-inseminação. Foi mensurado o diâmetro biparietal (DBP) fetal e aplicado na fórmula. Os resultados foram expressos na forma de média e erro padrão. O DBP foi de 2,14±0,02 e a IG correspondeu a 52 dias. A fórmula não tem confiabilidade para estimar a IG nesta raça.(AU)

The ultrasound gestational follow-up allows the evaluation of the development of the concept and the estimation of gestational age (GI). However, the Nyland and Mattoon formula for calculating the GI was developed in the Beagle. Brachycephalic dogs such as the French Bulldog have a greater predisposition to dystocia, which makes the correct estimation of GI even more important. The objective of this study was to evaluate the Nyland and Mattoon formula for dating GI in French Bulldog. Five bitches were submitted to abdominal ultrasound at 55 days post-insemination. The fetal biparietal diameter (DBP) was measured and applied in the formula. The results were expressed as mean and standard error. DBP was 2.14±0.02 and IG corresponded to 52 days. The formula has no reliability to estimate GI in this breed.(AU)

Pseudomicetoma dermatofítico disseminado em um gato persa - Relato de caso / Dermatophytic pseudomycetoma disseminated in a persian cat - Case report / Pseudomicetoma dermatofitos diseminada en un gato persa - Relato de caso

O pseudomicetoma dermatofítico (PMD) é uma profunda infecção dérmica, causada por fungos, sendo o Microsporum canis o agente etiológico mais frequentemente envolvido. O objetivo do presente trabalho é relatar um caso de pseudomicetoma em um gato persa e alertar os médicos veterinários para incluir a doença como diagnóstico diferencial em dermatopatias em gatos, principalmente os da raça persa. Um felino macho, da raça Persa, de três anos, veio para atendimento apresentando 22 nódulos subcutâneos, distribuídos em todo corpo, com diâmetros que variavam de 2 a 8 centímetros, inclusive dentro do pavilhão auricular direito. Realizou-se raspado cutâneo e citologia aspirativa por agulha fina, obtendo resultados pouco conclusivos. Após a realização de exérese cirúrgica de grande parte dos nódulos e encaminhamento para exame histopatológico, obteve-se o diagnóstico de PMD. Após tratamento com itraconazol 10mg/kg SID por 5 meses houve completa remissão do quadro. A excisão cirúrgica foi essencial para a resolução do quadro, que juntamente com o itraconazol sistêmico tornou o tratamento efetivo para o PMD.(AU)

Dermatophytic pseudomycetoma (DPM) is a deep skin infection. Microsporum canis is the etiologic agent most frequently involved in DPM. The purpose of this study is to report a case of DPM in a Persian cat and suggest the veterinarians to include the disease as a differential diagnosis in skin diseases in Persian cats. A male Persian cat, 3 years old, came to be attended at the Veterinary Hospital. The patient was presenting 22 subcutaneous nodules measuring 2-8 cm in diameter, in different parts of the body, including inside the right ear. Skin scraping and fine needle aspiration cytology were performed, yielding inconclusive results. After performing the surgical removal of most of the nodules and a skin biopsy, the diagnosis of DPM was obtained. The cat had a complete remission of the disease after the treatment with itraconazole 10mg/kg SID for 5 months. It is important to emphasize that, as a therapeutic point of view, surgical excision is considered essential, but not enough to establish a permanent cure. Since there can be recurrence, the systemic use of itraconazole is indicated to efficiently cure animals presenting DPM.(AU)

El pseudomicetoma dermatofitos (PMD) es una infección profunda de la piel causada por hongos, siendo el Microsporum canis el agente etiológico implicado con mayor frecuencia. El objetivo de este trabajo es presentar un caso de pseudomicetoma en un gato persa y alertar los médicos veterinarios para incluir la enfermedad como un diagnóstico diferencial de enfermedades de la piel en gatos, especialmente la raza persa. Un gato macho, de la raza persa, de tres años, llegó a la asistencia presentando 22 nódulos subcutáneos, distribuidos por todo el cuerpo, con diámetros que variaban de 2 a 8 cm, inclusive dentro de la oreja derecha. Se afeitó la piel y con citología por aspiración, con aguja fina, se obtuvo resultados poco conclusivos. Después de realizar la extirpación quirúrgica de la mayor parte de los nódulos y enrutamiento para el examen histopatológico, se ha obtenido el diagnóstico de PMD. Tras el tratamiento con itraconazol l0mg/kg SID, por cinco meses, hubo remisión completa. La escisión quirúrgica fue esencial para la resolución del cuadro, que junto con itraconazol sistémico se convirtió en un tratamiento eficaz para el PMD.(AU)

Pseudomicetoma dermatofítico disseminado em um gato persa - Relato de caso / Dermatophytic pseudomycetoma disseminated in a persian cat - Case report / Pseudomicetoma dermatofitos diseminada en un gato persa - Relato de caso

O pseudomicetoma dermatofítico (PMD) é uma profunda infecção dérmica, causada por fungos, sendo o Microsporum canis o agente etiológico mais frequentemente envolvido. O objetivo do presente trabalho é relatar um caso de pseudomicetoma em um gato persa e alertar os médicos veterinários para incluir a doença como diagnóstico diferencial em dermatopatias em gatos, principalmente os da raça persa. Um felino macho, da raça Persa, de três anos, veio para atendimento apresentando 22 nódulos subcutâneos, distribuídos em todo corpo, com diâmetros que variavam de 2 a 8 centímetros, inclusive dentro do pavilhão auricular direito. Realizou-se raspado cutâneo e citologia aspirativa por agulha fina, obtendo resultados pouco conclusivos. Após a realização de exérese cirúrgica de grande parte dos nódulos e encaminhamento para exame histopatológico, obteve-se o diagnóstico de PMD. Após tratamento com itraconazol 10mg/kg SID por 5 meses houve completa remissão do quadro. A excisão cirúrgica foi essencial para a resolução do quadro, que juntamente com o itraconazol sistêmico tornou o tratamento efetivo para o PMD.(AU)

Dermatophytic pseudomycetoma (DPM) is a deep skin infection. Microsporum canis is the etiologic agent most frequently involved in DPM. The purpose of this study is to report a case of DPM in a Persian cat and suggest the veterinarians to include the disease as a differential diagnosis in skin diseases in Persian cats. A male Persian cat, 3 years old, came to be attended at the Veterinary Hospital. The patient was presenting 22 subcutaneous nodules measuring 2-8 cm in diameter, in different parts of the body, including inside the right ear. Skin scraping and fine needle aspiration cytology were performed, yielding inconclusive results. After performing the surgical removal of most of the nodules and a skin biopsy, the diagnosis of DPM was obtained. The cat had a complete remission of the disease after the treatment with itraconazole 10mg/kg SID for 5 months. It is important to emphasize that, as a therapeutic point of view, surgical excision is considered essential, but not enough to establish a permanent cure. Since there can be recurrence, the systemic use of itraconazole is indicated to efficiently cure animals presenting DPM.(AU)

El pseudomicetoma dermatofitos (PMD) es una infección profunda de la piel causada por hongos, siendo el Microsporum canis el agente etiológico implicado con mayor frecuencia. El objetivo de este trabajo es presentar un caso de pseudomicetoma en un gato persa y alertar los médicos veterinarios para incluir la enfermedad como un diagnóstico diferencial de enfermedades de la piel en gatos, especialmente la raza persa. Un gato macho, de la raza persa, de tres años, llegó a la asistencia presentando 22 nódulos subcutáneos, distribuidos por todo el cuerpo, con diámetros que variaban de 2 a 8 cm, inclusive dentro de la oreja derecha. Se afeitó la piel y con citología por aspiración, con aguja fina, se obtuvo resultados poco conclusivos. Después de realizar la extirpación quirúrgica de la mayor parte de los nódulos y enrutamiento para el examen histopatológico, se ha obtenido el diagnóstico de PMD. Tras el tratamiento con itraconazol l0mg/kg SID, por cinco meses, hubo remisión completa. La escisión quirúrgica fue esencial para la resolución del cuadro, que junto con itraconazol sistémico se convirtió en un tratamiento eficaz para el PMD.(AU)

Hemoparasitoses associadas a doenças uterinas em cadela Golden Retriever Relato de caso / Hemoparasitoses associated with uterine diseases in Golden Retriever bitch Case report

As most common uterine diseases encountered in clinical small animal are complex cystic endometrialhyperplasia and its evolution to hydrometra, mucometra and pyometra. His clinical signs are common to otherdiseases that affect dogs very often in northeastern Brazil, as hemoparasitoses as ehrlichiosis and babesiosis. Whenthese conditions occur simultaneously, the diagnosis and, consequently, treatment may be impaired. Thus, this workrelated to the case of a bitch who presents a research on the subject, ehrlichiosis and babesiosis, describing thetreatment and the implications of the simultaneous occurrence of diseases in the effectiveness of treatments.(AU)

Correlação entre os parâmetros espermáticos e dosagem de testosterona sérica em gatos / Correlation between sperm parameters and measurement of serum testosterone in cats

The objective of this study was to evaluate the quality of epididymal spermatozoa recovered fresh andits correlation with the serum testosterone. Sperm was recovered from 8 feline epididymis and evaluated formotility, vigor, functionality, viability, morphology and concentration. Blood samples were obtained fromvenipuncture, and the blood was placed in tubes and centrifuged, and the serum obtained was placed in tubesand transfered to freezer until processing. It was observed that the values from the sperm assessments wereaccording to the literature, the amount of testosterone concentration was lower than that seen in the literatureand we found that this hormone positively influences the motility and vigor and the percentage of normal spermis inversely proportional to the concentration of serum testosterone.(AU)

Correlação entre os parâmetros espermáticos e dosagem de testosterona sérica em gatos / Correlation between sperm parameters and measurement of serum testosterone in cats

The objective of this study was to evaluate the quality of epididymal spermatozoa recovered fresh andits correlation with the serum testosterone. Sperm was recovered from 8 feline epididymis and evaluated formotility, vigor, functionality, viability, morphology and concentration. Blood samples were obtained fromvenipuncture, and the blood was placed in tubes and centrifuged, and the serum obtained was placed in tubesand transfered to freezer until processing. It was observed that the values from the sperm assessments wereaccording to the literature, the amount of testosterone concentration was lower than that seen in the literatureand we found that this hormone positively influences the motility and vigor and the percentage of normal spermis inversely proportional to the concentration of serum testosterone.

Hemoparasitoses associadas a doenças uterinas em cadela Golden Retriever – Relato de caso / Hemoparasitoses associated with uterine diseases in Golden Retriever bitch – Case report

As most common uterine diseases encountered in clinical small animal are complex cystic endometrialhyperplasia and its evolution to hydrometra, mucometra and pyometra. His clinical signs are common to otherdiseases that affect dogs very often in northeastern Brazil, as hemoparasitoses as ehrlichiosis and babesiosis. Whenthese conditions occur simultaneously, the diagnosis and, consequently, treatment may be impaired. Thus, this workrelated to the case of a bitch who presents a research on the subject, ehrlichiosis and babesiosis, describing thetreatment and the implications of the simultaneous occurrence of diseases in the effectiveness of treatments.

Avaliação do peso ao nascimento de machos e fêmeas e da prolificidade por sexo em cães da raça Buldogue Francês / Evaluation of males and females birth weight and prolificity by sex of French Bulldogs

The aimed of the study was to report the prolificacy, check if there is difference in the proportion ofmales and females and weight between the sexes of the breed puppies French Bulldog soon after birth. Seventypuppies from 18 litters were evaluated. All animals were evaluated on a maximum of 12 hours after birth.Weight was measured in grams in electronic balance. Data were expressed as mean and standard deviation andcompared by T-test. The average weight was 226 ± 29.46 g. There was no statistical difference in birth weightbetween males (230.40 ± 34.72 g) and females (223.60 ± 23.49 g). The prolificacy was 3.89 ± 1.71. There wereno differences in the number of male offspring (1.89 ± 1.18) and females per litter (2.00 ± 1.33). We concludethat gender does not influence the birth weight, and the male: female proportion is similar.(AU)

Avaliação histomorfológica do tecido testicular de gatos domésticos impúberes após vitrificação / Histomorphological evaluation of testicular tissue from prepubertal cats after vitrification

The aim of this study was to evaluate prepubertal cats testicular tissue histomorphology aftervitrification. Five testicular pairs from five prepubertal cats were used. The fragments were distributed in thecontrol group and vitrification group containing association of the cryoprotectants ethylene glycol and glycerolin a final concentration of 5.6M. Testicular fragments of the control group were fixed in Bouin solution,processed for histomorphological evaluation and stained with hematoxylin-eosin. Then, the fragments ofvitrification group were subjected to a solid surface vitrification and stored in liquid nitrogen. The fragmentswere thawed and processed for histology similarly to the control group. The control group was superior to thevitrification group regarding to the basal membrane separation cell, cell differentiation, visibility of the nucleusand nuclear condensation. Regarding the shrinkage of the basal membrane, there were no differences betweenthe groups. Morphological analysis of prepubertal cats testicular tissue after vitrification can contribute toimprove the reproductive biotechnologies applied to felines.(AU)

Avaliação do peso ao nascimento de machos e fêmeas e da prolificidade por sexo em cães da raça Buldogue Francês / Evaluation of males and females birth weight and prolificity by sex of French Bulldog’s

The aimed of the study was to report the prolificacy, check if there is difference in the proportion ofmales and females and weight between the sexes of the breed puppies French Bulldog soon after birth. Seventypuppies from 18 litters were evaluated. All animals were evaluated on a maximum of 12 hours after birth.Weight was measured in grams in electronic balance. Data were expressed as mean and standard deviation andcompared by T-test. The average weight was 226 ± 29.46 g. There was no statistical difference in birth weightbetween males (230.40 ± 34.72 g) and females (223.60 ± 23.49 g). The prolificacy was 3.89 ± 1.71. There wereno differences in the number of male offspring (1.89 ± 1.18) and females per litter (2.00 ± 1.33). We concludethat gender does not influence the birth weight, and the male: female proportion is similar.

Avaliação histomorfológica do tecido testicular de gatos domésticos impúberes após vitrificação / Histomorphological evaluation of testicular tissue from prepubertal cats after vitrification

The aim of this study was to evaluate prepubertal cats’ testicular tissue histomorphology aftervitrification. Five testicular pairs from five prepubertal cats were used. The fragments were distributed in thecontrol group and vitrification group containing association of the cryoprotectants ethylene glycol and glycerolin a final concentration of 5.6M. Testicular fragments of the control group were fixed in Bouin solution,processed for histomorphological evaluation and stained with hematoxylin-eosin. Then, the fragments ofvitrification group were subjected to a solid surface vitrification and stored in liquid nitrogen. The fragmentswere thawed and processed for histology similarly to the control group. The control group was superior to thevitrification group regarding to the basal membrane separation cell, cell differentiation, visibility of the nucleusand nuclear condensation. Regarding the shrinkage of the basal membrane, there were no differences betweenthe groups. Morphological analysis of prepubertal cats’ testicular tissue after vitrification can contribute toimprove the reproductive biotechnologies applied to felines.

Métodos contraceptivos em gatas domésticas – revisão de literatura / Review on contraceptive methods in domestic queens

Although ovariohysterectomy (OSH) or ovariectomy are methods of choice for contraception in queens, genectic valuable animals or animals that will be used for reproduction in future require safe and reversible methods for contraception. Many contraceptive methods have been used in cats, such as progestagens, androgens, imunocontraception and GnRH agonists. Although effective, many of those contraceptives are associated with serious side effects. Know the mechanism of action, main indication and possible side effects may help during the choice of the contraceptive method.

Apesar da ovariosalpingohisterectomia (OSH) ou ovariectomia serem os métodos contraceptivos de escolha para gatas domésticas, o manejo reprodutivo de animais geneticamente valiosos ou que ainda serão utilizados para reprodução requer métodos contraceptivos seguros e reversíveis. Diversos métodos contraceptivos reversíveis já foram utilizados em gatas entre eles os progestágenos, os andrógenos, os imunocontraceptivos e os agonistas do GnRH. Apesar de eficientes, muitos desses contraceptivos estão associados a efeitos colaterais sérios. Conhecer o mecanismo de ação, as principais indicações e os possíveis efeitos colaterais auxilia na escolha do contraceptivo a ser utilizado.

Métodos contraceptivos em gatas domésticas revisão de literatura / Review on contraceptive methods in domestic queens

Although ovariohysterectomy (OSH) or ovariectomy are methods of choice for contraception in queens, genectic valuable animals or animals that will be used for reproduction in future require safe and reversible methods for contraception. Many contraceptive methods have been used in cats, such as progestagens, androgens, imunocontraception and GnRH agonists. Although effective, many of those contraceptives are associated with serious side effects. Know the mechanism of action, main indication and possible side effects may help during the choice of the contraceptive method.(AU)

Apesar da ovariosalpingohisterectomia (OSH) ou ovariectomia serem os métodos contraceptivos de escolha para gatas domésticas, o manejo reprodutivo de animais geneticamente valiosos ou que ainda serão utilizados para reprodução requer métodos contraceptivos seguros e reversíveis. Diversos métodos contraceptivos reversíveis já foram utilizados em gatas entre eles os progestágenos, os andrógenos, os imunocontraceptivos e os agonistas do GnRH. Apesar de eficientes, muitos desses contraceptivos estão associados a efeitos colaterais sérios. Conhecer o mecanismo de ação, as principais indicações e os possíveis efeitos colaterais auxilia na escolha do contraceptivo a ser utilizado.(AU)

Avaliação de espermatozoides epididimários de gatos domésticos após recuperação com ACP-117c ou Tris, adição de gema e refrigeração a 4 °C por 24h / Epididymal sperm evaluation of domestic cats after recovery with ACP-117c or Tris egg yolk addition and cooling at 4 °C for 24h

The aim of this study was to evaluate the quality of spermatozoa recovered from domestic cats epididymis using the ACP-117c or Tris extenders added by egg yolk and cooled for 24h. Four cats were used. The animals were submitted to bilateral orchiectomy, obtaining the testis epididymis complexes (TEC). The TEC were stored in 0.9% saline solution and cooled at 4 °C for 2h. Then the spermatozoa were recovered with ACP-117c or Tris and evaluated for motility and vigor. Subsequently, 80µl of the dilution were placed in a 1.5 mL micro tube together with 20µl of egg yolk and the cells were reassessed. Soon after, an aliquot was stored in a refrigerator at 4 °C for 24 hours and then reassessed. The total motility and sperm vigor using the ACP-117c extender were significantly lower in the group refrigerated for 24h when compared to the fresh group. The motility and vigor declined in the refrigerated group with Tris for 24h as compared to other groups. The motility with Tris was higher than the motility with ACP-117c after 24h of cooling. The epididymal spermatic cells of domestic cats can maintain motility parameters and vigor required for further use after addition of egg yolk, however, further studies should be developed with ACP-117c in the post-cooling time in order to obtain better results.

O objetivo desse trabalho foi avaliar a qualidade de espermatozoides recuperados do epidídimo de gatos domésticos utilizando os diluidores ACP-117c ou Tris adicionados de gema de ovo e refrigerados por 24h. Foram utilizados quatro gatos. Os animais foram submetidos à orquiectomia bilateral, obtendo-se os complexos testículo-epidídimo (CTE). Os CTE foram acondicionados em solução fisiológica a 0,9% e refrigerados a 4 °C durante 2h. Em seguida, os espermatozoides foram recuperados com ACP-117c ou Tris e avaliados quanto à motilidade e ao vigor. Posteriormente, 80µl da diluição foram acondicionados em um microtubo de 1,5mL juntamente com 20µl de gema de ovo e as células reavaliadas. Logo após, uma alíquota foi armazenada sob refrigeração a 4 ºC por 24h e então novamente avaliada. A motilidade total e o vigor espermático utilizando o diluidor ACP-117c foram significativamente inferiores no grupo refrigerado por 24h quando comparados ao grupo fresco. A motilidade e o vigor declinaram no grupo refrigerado com o Tris por 24h em comparação aos outros grupos. A motilidade com o Tris foi superior à motlilidade com o ACP-117c após 24h de refrigeração. As células espermáticas epididimárias de gatos domésticos conseguem manter os parâmetros de motilidade e vigor necessários para posterior utilização após adição da gema de ovo, entretanto, no período pós-refrigeração devem ser desenvolvidos novos estudos com ACP-117c com vistas a melhores resultados.

Criopreservação de sêmen e inseminação artificial em cães / Cryopreservation of semen and artificial insemination in dogs

Diante de um crescente mercado que requisita produtos e serviços para cães, o presente trabalho objetiva descrever as principais etapas e resultados obtidos na criopreservação de sêmen e inseminação artificiais na espécie canina.

Faced with a growing market that demands products and services for dogs, this paper aims to describe the main steps and results in cryopreservation of semen and artificial insemination in dogs.

Desafios para o desenvolvimento da tecnologia da criopreservação de sêmen felino / Challenges for the development of feline semen cryopreservation technology

O objetivo deste trabalho é levantar os pontos mais importantes que podem comprometer o resultado final do procedimento de criopreservação seminal em felinos.

The aim of this work is to raise the most important points that can affect the outcome of semen cryopreservation procedure in cats.

Criopreservação de sêmen e inseminação artificial em cães / Cryopreservation of semen and artificial insemination in dogs

Diante de um crescente mercado que requisita produtos e serviços para cães, o presente trabalho objetiva descrever as principais etapas e resultados obtidos na criopreservação de sêmen e inseminação artificiais na espécie canina.(AU)

Faced with a growing market that demands products and services for dogs, this paper aims to describe the main steps and results in cryopreservation of semen and artificial insemination in dogs.(AU)

Desafios para o desenvolvimento da tecnologia da criopreservação de sêmen felino / Challenges for the development of feline semen cryopreservation technology

O objetivo deste trabalho é levantar os pontos mais importantes que podem comprometer o resultado final do procedimento de criopreservação seminal em felinos.(AU)

The aim of this work is to raise the most important points that can affect the outcome of semen cryopreservation procedure in cats.(AU)