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1.
Ciênc. anim. bras. (Impr.) ; 23: e-72715P, 2022. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1404210

Resumo

Curcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 µM, 20 µM, 50 µM, 100 µM, and 1000 µM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 µM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases.


A Curcuma longa L., planta conhecida popularmente como açafrão, tem sido amplamente estudada por suas diversas propriedades terapêuticas, incluindo a ação antineoplásica. O extrato etanólico da planta contém diversos compostos fenólicos, com destaque para a curcumina. O osteossarcoma é um tumor ósseo predominante em cães e humanos, caracterizado por apresentar alto potencial metastático e prognóstico desfavorável. Procurou-se investigar os efeitos de diferentes concentrações de curcumina do extrato etanólico de açafrão sobre células de osteossarcoma canino de cultura estabelecida. As células foram cultivadas e submetidas ao tratamento com extrato com diferentes concentrações de curcumina (0, 10 µM, 20 µM, 50 µM, 100 µM e 1000 µM) e tempos de exposição (24h, 48h e 72h) pelo EEA. Inicialmente, foram realizados: técnica de redução do tetrazólio (MTT) e cálculo da IC50. Posteriormente, após o tratamento com o extrato, realizou-se o ensaio de imunocitoquímica para verificar a expressão de p53 mutada e estudar o potencial proliferativo das células malignas; Bcl-2, com intuito de averiguar o estímulo de via antiapoptótica; e o marcador Ki-67, que sinaliza aumento no grau de malignidade. O extrato promoveu proliferação de células de osteossarcoma canino, com incremento de até 3819,74% na concentração de 50µM de curcumina. O composto também alterou a expressão das proteínas p53 mutante e Ki-67 significativamente, mas não alterou a expressão de Bcl-2, mostrando que não induziu a via antiapoptótica mediada por esta. Estes resultados demonstram que o extrato etanólico do açafrão apresenta potencial proliferativo sobre células de osteossarcoma canino, sugerindo a necessidade de conscientização e conhecimento dos reais efeitos de determinados compostos naturais, considerados seguros ao serem utilizados como tratamento de diversas enfermidades.


Assuntos
Animais , Cães , Neoplasias Ósseas/veterinária , Osteossarcoma/veterinária , Curcumina , Curcuma , Proliferação de Células/efeitos dos fármacos , Técnicas In Vitro/veterinária , Doenças do Cão , Compostos Fitoquímicos
2.
Acta sci., Biol. sci ; 43: e52783, 2021. graf, tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1460988

Resumo

Osteosarcoma is the most commonly diagnosed malignant bone tumor in humans, with a higher incidence in children and young people. It is highly aggressive and has a high metastatic potential. Its treatment is based on both chemotherapy and surgical intervention. However, currently used chemotherapeutic agents, such as doxorubicin, have several adverse effects on the patient. Therefore, there is a growing demand for new chemotherapeutic agents that stimulate new researches, such as those involving compounds extracted from plants, such as the gabirobeira. In this study, we aimed to evaluate the cytotoxic effects of ethanolic extract, both crude and ethyl acetate, of gabirobeira leaves on osteosarcoma cells in vitro. Cytotoxicity was evaluated using the Trypan blue exclusion method and the IC50 values were calculated using the tetrazolium reduction method. The ethanolic extract of gabirobeira leaves showed a cytotoxic effect on osteosarcoma cells in vitro. The group treated with the crude extract at 1. 0& 956;L mL-1 concentration for 48 hours showed higher cytotoxicity and the lowest IC50 value for this extract was found in the 24 to 48 hours interval. The ethanolic extract of gabirobeira leaves is cytotoxic for osteosarcoma cells.


Assuntos
Etanol , Myrtaceae/química , Osteossarcoma/química , Técnicas In Vitro , Óleos Voláteis/análise
3.
Acta Sci. Biol. Sci. ; 43: e52783, 2021. graf, tab
Artigo em Inglês | VETINDEX | ID: vti-764597

Resumo

Osteosarcoma is the most commonly diagnosed malignant bone tumor in humans, with a higher incidence in children and young people. It is highly aggressive and has a high metastatic potential. Its treatment is based on both chemotherapy and surgical intervention. However, currently used chemotherapeutic agents, such as doxorubicin, have several adverse effects on the patient. Therefore, there is a growing demand for new chemotherapeutic agents that stimulate new researches, such as those involving compounds extracted from plants, such as the gabirobeira. In this study, we aimed to evaluate the cytotoxic effects of ethanolic extract, both crude and ethyl acetate, of gabirobeira leaves on osteosarcoma cells in vitro. Cytotoxicity was evaluated using the Trypan blue exclusion method and the IC50 values were calculated using the tetrazolium reduction method. The ethanolic extract of gabirobeira leaves showed a cytotoxic effect on osteosarcoma cells in vitro. The group treated with the crude extract at 1. 0& 956;L mL-1 concentration for 48 hours showed higher cytotoxicity and the lowest IC50 value for this extract was found in the 24 to 48 hours interval. The ethanolic extract of gabirobeira leaves is cytotoxic for osteosarcoma cells.(AU)


Assuntos
Técnicas In Vitro , Myrtaceae/química , Óleos Voláteis/análise , Osteossarcoma/química , Etanol
4.
Acta sci., Biol. sci ; 43: e52784, 2021. tab, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1460985

Resumo

The selective activity of an antineoplastic drug is related to its ability to promote cytotoxic action on tumor cells and preserve the integrity of non-neoplastic cells. Beta-lapachone is extracted from the sawdust of Ipe wood, a thick bark tree from the Ipe wood found in the Brazilian Cerrado biome. This study aimed to evaluate the cytotoxic action of beta-lapachone in an endothelial cell line. The EA.hy926 cells were seeded in two groups, G1 and G2, cultured and exposed to beta-lapachone at concentrations of 0.0, 0.01, 0.03, 0.1, 0.3, 1 and 3 & 956;M for 24 hours. G1 remained under normal cultivation conditions and G2 was subjected to oxidative stress through an ischemia and reperfusion assay, in a deoxygenated sealed chamber. The cytotoxicity assay was performed using the tetrazolium reduction method. In G1, the cytotoxicity ranged from 0.0 to 10.0%; and in G2 between 0.0 and 6.3%. No statistically significant difference was observed between the obtained values. Moreover, we found no cytotoxic action of beta-lapachone on endothelial cells, and the results point out that the drug might have preserved the cell’s integrity against oxidative stress under the conditions of this experiment. This promising result suggests the possibility of beta-lapachone as a chemotherapy drug with selective activity.


Assuntos
Células Endoteliais/fisiologia , Células Endoteliais/química , Naftoquinonas , Testes Imunológicos de Citotoxicidade
5.
Acta Sci. Biol. Sci. ; 43: e52784, 2021. tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-764594

Resumo

The selective activity of an antineoplastic drug is related to its ability to promote cytotoxic action on tumor cells and preserve the integrity of non-neoplastic cells. Beta-lapachone is extracted from the sawdust of Ipe wood, a thick bark tree from the Ipe wood found in the Brazilian Cerrado biome. This study aimed to evaluate the cytotoxic action of beta-lapachone in an endothelial cell line. The EA.hy926 cells were seeded in two groups, G1 and G2, cultured and exposed to beta-lapachone at concentrations of 0.0, 0.01, 0.03, 0.1, 0.3, 1 and 3 & 956;M for 24 hours. G1 remained under normal cultivation conditions and G2 was subjected to oxidative stress through an ischemia and reperfusion assay, in a deoxygenated sealed chamber. The cytotoxicity assay was performed using the tetrazolium reduction method. In G1, the cytotoxicity ranged from 0.0 to 10.0%; and in G2 between 0.0 and 6.3%. No statistically significant difference was observed between the obtained values. Moreover, we found no cytotoxic action of beta-lapachone on endothelial cells, and the results point out that the drug might have preserved the cells integrity against oxidative stress under the conditions of this experiment. This promising result suggests the possibility of beta-lapachone as a chemotherapy drug with selective activity.(AU)


Assuntos
Testes Imunológicos de Citotoxicidade , Naftoquinonas , Células Endoteliais/química , Células Endoteliais/fisiologia
6.
Pesqui. vet. bras ; 36(11): 1127-1131, Nov. 2016. tab, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-842011

Resumo

New World primates Sapajus sp. unexpectedly display cognitive aspects, tool use, social behavior, memory and anatomical aspects similar to Old World primates, such as chimpanzees and baboons. Convergent evolutionary aspects must have occurred between Sapajus and Old World primates and should be verified not only in terms of behavior analysis, but also of anatomical structure. The pelvic region can provide data for evolutionary verification trends, since taking standing position is one of the characteristics associated to the use of tools by early humans and pongids. We used eight specimens of Sapajus libidinosus to describe the deep muscular structure of the pelvis. Unlike humans, the gluteus medius muscle in S. libidinosus is completely covered by the gluteus maximus and elongated as compared to humans and chimpanzees, putatively by the elongated pelvis of S. libidinosus. Considering origin and insertion, the gluteus maximus muscle resembles more its counterpart in baboons than in humans and chimpanzees, since this muscle in baboons is associated to semibiped posture and to the tail. Gluteus minimum, piriformis, superior gemellus, internal shutter, gemellus and lower square muscles are positioned in this order in relation to the cranial-caudal axis, with all of its tendons converging for a common insertion in the greater trochanter. The muscles of the gluteal region of S. libidinosus are similar to the baboon, especially regarding the gluteus maximus, which points to the evolutionary kinship of these animals.(AU)


Os Sapajus sp apresentam aspectos cognitivos, uso de ferramentas, comportamento social e memória, além de aspectos anatômicos, similares à primatas do Velho Mundo, como chimpanzés e babuínos, fatos inesperados para estes animais, que são primatas do Novo Mundo. Aspectos evolutivos convergentes devem ter ocorrido entre Sapajus e primatas do Velho Mundo que devem ser verificados não somente em termos de análise do comportamento, mas da estrutura anatômica. A região pélvica pode fornecer dados para a verificação de tendências evolucionárias, pois assumir a posição bípede é uma das características associadas ao uso de ferramentas pelos humanos primitivos e pongídeos. Para descrever a estrutura muscular profunda da pelve, foram utilizados oito espécimes de Sapajus libidinosus. O músculo glúteo médio em S. libidinosus, diferente de humanos, é totalmente recoberto pelo glúteo máximo, é alongado em relação aos humanos e chimpanzés, putativamente pela pelve alongada dos S. libidinosus. O músculo glúteo máximo se assemelha, considerando origem e inserção mais aos babuínos do que em humanos e chimpanzés, uma vez que em babuínos esse músculo está associado à postura semibípede e à cauda. Os músculos glúteo mínimo, piriforme, gêmeo superior, obturador interno, gêmeo inferior e quadrado estão posicionados nesta ordem em relação ao eixo crânio-caudal com todos seus tendões convergindo para uma inserção comum no trocânter maior. Os músculos da região glútea de S. libidinosus são semelhantes ao babuíno principalmente no que se refere ao músculo glúteo máximo, o que reflete o parentesco evolutivo desses animais.(AU)


Assuntos
Animais , Nádegas/anatomia & histologia , Cebus/anatomia & histologia , Músculos/anatomia & histologia , Pesos e Medidas Corporais/veterinária , Primatas/anatomia & histologia
7.
Pesqui. vet. bras ; 36(11): 1127-1131, nov. 2016. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-683708

Resumo

New World primates Sapajus sp. unexpectedly display cognitive aspects, tool use, social behavior, memory and anatomical aspects similar to Old World primates, such as chimpanzees and baboons. Convergent evolutionary aspects must have occurred between Sapajus and Old World primates and should be verified not only in terms of behavior analysis, but also of anatomical structure. The pelvic region can provide data for evolutionary verification trends, since taking standing position is one of the characteristics associated to the use of tools by early humans and pongids. We used eight specimens of Sapajus libidinosus to describe the deep muscular structure of the pelvis. Unlike humans, the gluteus medius muscle in S. libidinosus is completely covered by the gluteus maximus and elongated as compared to humans and chimpanzees, putatively by the elongated pelvis of S. libidinosus. Considering origin and insertion, the gluteus maximus muscle resembles more its counterpart in baboons than in humans and chimpanzees, since this muscle in baboons is associated to semibiped posture and to the tail. Gluteus minimum, piriformis, superior gemellus, internal shutter, gemellus and lower square muscles are positioned in this order in relation to the cranial-caudal axis, with all of its tendons converging for a common insertion in the greater trochanter. The muscles of the gluteal region of S. libidinosus are similar to the baboon, especially regarding the gluteus maximus, which points to the evolutionary kinship of these animals.(AU)


Os Sapajus sp apresentam aspectos cognitivos, uso de ferramentas, comportamento social e memória, além de aspectos anatômicos, similares à primatas do Velho Mundo, como chimpanzés e babuínos, fatos inesperados para estes animais, que são primatas do Novo Mundo. Aspectos evolutivos convergentes devem ter ocorrido entre Sapajus e primatas do Velho Mundo que devem ser verificados não somente em termos de análise do comportamento, mas da estrutura anatômica. A região pélvica pode fornecer dados para a verificação de tendências evolucionárias, pois assumir a posição bípede é uma das características associadas ao uso de ferramentas pelos humanos primitivos e pongídeos. Para descrever a estrutura muscular profunda da pelve, foram utilizados oito espécimes de Sapajus libidinosus. O músculo glúteo médio em S. libidinosus, diferente de humanos, é totalmente recoberto pelo glúteo máximo, é alongado em relação aos humanos e chimpanzés, putativamente pela pelve alongada dos S. libidinosus. O músculo glúteo máximo se assemelha, considerando origem e inserção mais aos babuínos do que em humanos e chimpanzés, uma vez que em babuínos esse músculo está associado à postura semibípede e à cauda. Os músculos glúteo mínimo, piriforme, gêmeo superior, obturador interno, gêmeo inferior e quadrado estão posicionados nesta ordem em relação ao eixo crânio-caudal com todos seus tendões convergindo para uma inserção comum no trocânter maior. Os músculos da região glútea de S. libidinosus são semelhantes ao babuíno principalmente no que se refere ao músculo glúteo máximo, o que reflete o parentesco evolutivo desses animais.(AU)


Assuntos
Animais , Cebus/anatomia & histologia , Nádegas/anatomia & histologia , Músculos/anatomia & histologia , Pesos e Medidas Corporais/veterinária , Primatas/anatomia & histologia
8.
Acta Sci. Biol. Sci. ; 35(2): 263-271, apr.-june.2013. graf, tab
Artigo em Inglês | VETINDEX | ID: vti-27121

Resumo

In Brazil, the knowledge on the use of medicine plants comes mainly from natives with African and European influences. With descriptive purposes and valuation of traditional knowledge, a study with induced questioning was conducted in the municipality of Goianلpolis, Goiلs State, to verify the plants considered to be medicinal by the local population. The data collection through structured interviews in the form of questionnaires from sixty inhabitants randomly selected. The study followed the ethical aspects and was approved by the Unievangélica Research and Ethics Committee. Most of the interviewed subjects was of female gender, older than 30 years and had already lived in the rural area for at least one phase of their lives, the use of leaves and the method of tea making and infusion were most mentioned, the plants most used were Lemon Balm, Mint, Chilean evergreen tree, elderberry, wormseed, Pennyroyal, and the most mentioned families are Asteraceae and Laminaceae. The knowledge about plants use was obtained, according most of interviewed, from family. In conclusion, the data observed in Goianلpolis, Goiلs State, corroborates of those observed by other researchers about the use of plants, the most used botanical families, the plant parts to be used and the form of preparation.(AU)


No Brasil, o conhecimento sobre o uso de plantas medicinais é proveniente principalmente dos indيgenas, com influências negras e européias. Com fins descritivos e de valorizaçمo do conhecimento tradicional, foi realizado um estudo de questionamento induzido no municيpio de Goianلpolis, Estado de Goiلs, para a verificaçمo das plantas consideradas de carلter medicinal usadas pela populaçمo desse municيpio. O estudo foi aprovado pelo Comitê de ةtica e Pesquisa da Unievangélica e foram entrevistados 60 moradores escolhidos através de sorteio A maioria foi do gênero feminino, maiores de 30 anos e jل residiram na zona rural em pelo menos uma fase da vida. O uso de folhas e o modo de preparo de chل e infusمo foram os mais citados, as plantas mais utilizadas foram erva-cidreira, hortelم, boldo, sabugueiro, erva-de-santa-maria e poejo e as famيlias mais citadas foram a Asteraceae e a Laminaceae, nesta ordem. O conhecimento sobre a utilizaçمo das plantas foi adquirido, segundo a maioria dos entrevistados, por informaçُes ensinadas na prَpria famيlia. Em conclusمo, os dados obtidos em Goianلpolis, Estado de Goiلs, estمo em acordo com o observado por outros pesquisadores sobre o uso de plantas medicinais, as famيlias de plantas mais citadas, as partes mais usadas das plantas e a forma de preparo.(AU)


Assuntos
Plantas Medicinais/classificação , Etnobotânica , Etnofarmacologia
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