Intercurrence of paratuberculosis in intradermal tuberculin test reactive cattle

Background: Bovine tuberculosis control programs are based on a standard diagnostic method, the intradermal test with purified protein derivatives, which is used to identify and eliminate diseased animals. Currently none of the tests available allow complete differentiation between infected and uninfected animals. The main limitations of the tests available are related to diagnostic sensitivity and specificity, which results in false positive reactions due to the existence of cross infections, and also false negative, inherent to the state of energy of some animals. The aim of this work was to study the intercurrence of paratuberculosis in tuberculosis reactive cattle by the comparative cervical test. Materials, Methods & Results: Three hundred and thirty four cattle were evaluated using the comparative cervical test (CCT) and serology for tuberculosis (TB) and paratuberculosis (PTB) ELISA IDEXX®. All of the animals testing positive by CCT were euthanized and necropsied. Fragments of lymph node, lung and intestine were collected and analyzed using histopathological techniques, with staining by Hematoxylin-Eosin (HE). Samples of lung and lymph nodes (retropharyngeal, submandibular, cervical and mediastinal) of the animals testing positive by CCT were evaluated using qPRC for M. bovis, and intestinal and mesenteric lymph nodes using PCR for PTB. Of the 334 cattle evaluated using the comparative cervical test, 16 were considered positive. No lesions suggestive of tuberculosis were found in the macroscopic inspection of the carcasses. The most evident anatomical and pathological finding was a thickening of intestinal mucosa, found in 12 of the 16 cattle submitted to necropsy. No microscopic lesions suggestive of TB were identified nor was the presence of M. bovis detected by qPCR. The main histopathological findings were observed in the small intestine and mesenteric lymph nodes and identified as enteritis, lymphangitis...

Prognostic factors in canine mammary carcinomas and HER2 expression relationship / Fatores de prognóstico em carcinomas mamários caninos e sua relação com expressão de HER2

Background: The human epidermal growth factor type 2 (HER2) receptor is a membrane glycoprotein tyrosine kinase. In woman, HER2 expression is diagnosed in 30% of breast carcinomas and it is associated with a worse prognosis, higher rate of recurrence and mortality. In the bitch, the HER2 overexpression in canine mammary tumors is still controversial and the prognostic value remains uncertain. Thus, we aimed to verify the HER2 expression in canine mammary carcinomas and relate it to the type and histological grade, lymph node metastasis and clinical staging. Materials, Methods & Results: Ninety bitches diagnosed with mammary carcinoma were included in this study. The inclusion criteria were bitches with complete clinical examination, thoracic radiographic examination and submitted unilateral or bilateral mastectomy. Ninety-nine samples of mammary carcinoma were used and the fragments of tumor and regional lymph nodes were fixed in 10% neutral formalin for histopathological and immunohistochemistry analysis. The lesions were evaluated by two pathologists and classified according to the type and histological grade. HER2 expression was performed by semi-quantitative analysis of the slides according to the HerceptTestTM (Dako) recommended score. Simple carcinomas were the most frequent (51.51%) followed by complex carcinomas (46.47%) and in situ carcinoma (2.02%). [...](AU)

Hemangiomas and hemangiosarcomas in dogs: retrospective study of 192 cases (2002-2014) / Hemangiomas e hemangiossarcomas em cães: estudo retrospectivo de 192 casos (2002-2014)

The hemangioma and hemangiosarcoma are mesenchymal neoplasms from the vascular endothelium, and the dog is the domestic animal more commonly affected. At the veterinary hospital of the Federal University of Uberlândia, we reviewed the files from the Animal Pathology Laboratory from 2002 to 2014. The epidemiological data and the frequency of dogs diagnosed with hemangioma and hemangiosarcoma were verified, correlated, and statistically tested. 64 cases of hemangioma and 128 cases of hemangiosarcoma were identified. For hemangiomas, there was no difference in frequency regarding sex and breed. The cutaneous hemangiomas were more frequent than visceral hemangiomas. Female dogs and adult animals were often affected by hemangiosarcoma, as well as the Pitbull breed. The hemangiosarcomas had been predominantly cutaneous and medium size tumour was the most frequent for this neoplasm.

O hemangioma e o hemangiossarcoma são neoplasias mesenquimais originárias do endotélio vascular e o cão é o animal doméstico mais frequentemente acometido. Os registros do Laboratório de Patologia Animal do Hospital Veterinário da Universidade Federal de Uberlândia de 2002 a 2014 foram revisados. Os dados epidemiológicos e a frequência de cães com diagnóstico de hemangioma e hemangiossarcoma foram verificados e estatisticamente comparados. Foram identificados 64 casos de hemangioma e 128 casos de hemangiossarcoma. Para os hemangiomas, não houve diferença na frequência de acordo com sexo e raça. Quanto à localização, os hemangiomas não viscerais foram os mais frequentes em relação aos viscerais. As cadelas e animais adultos e idosos foram frequentemente acometidos por hemangiossarcoma, assim como a raça Pitbull. Os hemangiossarcomas apresentaram predominantemente tamanho médio e localização cutânea, ou seja, não visceral.

Prognostic factors in canine mammary carcinomas and HER2 expression relationship / Fatores de prognóstico em carcinomas mamários caninos e sua relação com expressão de HER2

Background: The human epidermal growth factor type 2 (HER2) receptor is a membrane glycoprotein tyrosine kinase. In woman, HER2 expression is diagnosed in 30% of breast carcinomas and it is associated with a worse prognosis, higher rate of recurrence and mortality. In the bitch, the HER2 overexpression in canine mammary tumors is still controversial and the prognostic value remains uncertain. Thus, we aimed to verify the HER2 expression in canine mammary carcinomas and relate it to the type and histological grade, lymph node metastasis and clinical staging. Materials, Methods & Results: Ninety bitches diagnosed with mammary carcinoma were included in this study. The inclusion criteria were bitches with complete clinical examination, thoracic radiographic examination and submitted unilateral or bilateral mastectomy. Ninety-nine samples of mammary carcinoma were used and the fragments of tumor and regional lymph nodes were fixed in 10% neutral formalin for histopathological and immunohistochemistry analysis. The lesions were evaluated by two pathologists and classified according to the type and histological grade. HER2 expression was performed by semi-quantitative analysis of the slides according to the HerceptTestTM (Dako) recommended score. Simple carcinomas were the most frequent (51.51%) followed by complex carcinomas (46.47%) and in situ carcinoma (2.02%). [...]

Retrospective study of mammary lesions in bitches - Uberlândia, MG, Brazil

Background: Mammary tumors are a type of neoplasia that are most commonly found in female dogs and are mostly malignant. The aim of this study, performed in the Laboratory of Veterinary Pathology of the Federal University of Uberlândia (LVP-FUU) from 2004 to 2014, was to determine the prevalence of mammary tumors in bitches and to verify the relationship between the epidemiological factors (age and breed) and clinicopathological aspects (ulceration, tumor size, and malignancy) in the occurrence of tumors.Materials, Methods & Results: A retrospective study was carried out using histopathological information retrieved from the LPV-UFU database. We collected the information on age and breed of female dogs, as well as about the location, macroscopic aspects, and histological diagnosis of mammary lesions. Only female dogs were considered for this study; a total of 911 histo-pathological protocols (with only one diagnosis) were analyzed along with 36 protocols that presented more than one diagnosis of mammary tumor. The age of animals ranged from one to 20 years, and the mean age was 9.99 years. The most affected breeds of dogs were: Cross breed (39.56% - 288/728) and Poodle (20.19% - 147/728). The inguinal glands were most affected by the malignant tumors (P< 0.05). A prevalence of tumors bigger than 5 cm in diameter (T3) was observed in the elderly animals (P= 0.0154) and in the inguinal mammary glands (P= 0.044). Simple carcinoma was the most frequent histological type.[...]

Retrospective study of mammary lesions in bitches - Uberlândia, MG, Brazil

Background: Mammary tumors are a type of neoplasia that are most commonly found in female dogs and are mostly malignant. The aim of this study, performed in the Laboratory of Veterinary Pathology of the Federal University of Uberlândia (LVP-FUU) from 2004 to 2014, was to determine the prevalence of mammary tumors in bitches and to verify the relationship between the epidemiological factors (age and breed) and clinicopathological aspects (ulceration, tumor size, and malignancy) in the occurrence of tumors.Materials, Methods & Results: A retrospective study was carried out using histopathological information retrieved from the LPV-UFU database. We collected the information on age and breed of female dogs, as well as about the location, macroscopic aspects, and histological diagnosis of mammary lesions. Only female dogs were considered for this study; a total of 911 histo-pathological protocols (with only one diagnosis) were analyzed along with 36 protocols that presented more than one diagnosis of mammary tumor. The age of animals ranged from one to 20 years, and the mean age was 9.99 years. The most affected breeds of dogs were: Cross breed (39.56% - 288/728) and Poodle (20.19% - 147/728). The inguinal glands were most affected by the malignant tumors (P< 0.05). A prevalence of tumors bigger than 5 cm in diameter (T3) was observed in the elderly animals (P= 0.0154) and in the inguinal mammary glands (P= 0.044). Simple carcinoma was the most frequent histological type.[...](AU)

Hemangiomas and hemangiosarcomas in dogs: retrospective study of 192 cases (2002-2014) / Hemangiomas e hemangiossarcomas em cães: estudo retrospectivo de 192 casos (2002-2014)

The hemangioma and hemangiosarcoma are mesenchymal neoplasms from the vascular endothelium, and the dog is the domestic animal more commonly affected. At the veterinary hospital of the Federal University of Uberlândia, we reviewed the files from the Animal Pathology Laboratory from 2002 to 2014. The epidemiological data and the frequency of dogs diagnosed with hemangioma and hemangiosarcoma were verified, correlated, and statistically tested. 64 cases of hemangioma and 128 cases of hemangiosarcoma were identified. For hemangiomas, there was no difference in frequency regarding sex and breed. The cutaneous hemangiomas were more frequent than visceral hemangiomas. Female dogs and adult animals were often affected by hemangiosarcoma, as well as the Pitbull breed. The hemangiosarcomas had been predominantly cutaneous and medium size tumour was the most frequent for this neoplasm.(AU)

O hemangioma e o hemangiossarcoma são neoplasias mesenquimais originárias do endotélio vascular e o cão é o animal doméstico mais frequentemente acometido. Os registros do Laboratório de Patologia Animal do Hospital Veterinário da Universidade Federal de Uberlândia de 2002 a 2014 foram revisados. Os dados epidemiológicos e a frequência de cães com diagnóstico de hemangioma e hemangiossarcoma foram verificados e estatisticamente comparados. Foram identificados 64 casos de hemangioma e 128 casos de hemangiossarcoma. Para os hemangiomas, não houve diferença na frequência de acordo com sexo e raça. Quanto à localização, os hemangiomas não viscerais foram os mais frequentes em relação aos viscerais. As cadelas e animais adultos e idosos foram frequentemente acometidos por hemangiossarcoma, assim como a raça Pitbull. Os hemangiossarcomas apresentaram predominantemente tamanho médio e localização cutânea, ou seja, não visceral.(AU)

HEMANGIOMAS E HEMANGIOSSARCOMAS EM CÃES: ESTUDO RETROSPECTIVO DE 192 CASOS (2002-2014)

Abstract The hemangioma and hemangiosarcoma are mesenchymal neoplasms from the vascular endothelium, and the dog is the domestic animal more commonly affected. At the veterinary hospital of the Federal University of Uberlândia, we reviewed the files from the Animal Pathology Laboratory from 2002 to 2014. The epidemiological data and the frequency of dogs diagnosed with hemangioma and hemangiosarcoma were verified, correlated, and statistically tested. 64 cases of hemangioma and 128 cases of hemangiosarcoma were identified. For hemangiomas, there was no difference in frequency regarding sex and breed. The cutaneous hemangiomas were more frequent than visceral hemangiomas. Female dogs and adult animals were often affected by hemangiosarcoma, as well as the Pitbull breed. The hemangiosarcomas had been predominantly cutaneous and medium size tumour was the most frequent for this neoplasm.

Resumo O hemangioma e o hemangiossarcoma são neoplasias mesenquimais originárias do endotélio vascular e o cão é o animal doméstico mais frequentemente acometido. Os registros do Laboratório de Patologia Animal do Hospital Veterinário da Universidade Federal de Uberlândia de 2002 a 2014 foram revisados. Os dados epidemiológicos e a frequência de cães com diagnóstico de hemangioma e hemangiossarcoma foram verificados e estatisticamente comparados. Foram identificados 64 casos de hemangioma e 128 casos de hemangiossarcoma. Para os hemangiomas, não houve diferença na frequência de acordo com sexo e raça. Quanto à localização, os hemangiomas não viscerais foram os mais frequentes em relação aos viscerais. As cadelas e animais adultos e idosos foram frequentemente acometidos por hemangiossarcoma, assim como a raça Pitbull. Os hemangiossarcomas apresentaram predominantemente tamanho médio e localização cutânea, ou seja, não visceral.

Evaluación imunohistoquímica de plasmocitoma cutáneo en un perro - reporte de un caso / Assessment immunohistochemistry of the cutaneous plasmacytoma in a dog - case report / Avaliação imunohistoquímica de plasmocitoma cutâneo em um cão: relato de caso

Los plasmacitomas son neoplasias poco comunes em los perros, procedentes de plasmocitos. Ocurre en cualquier tejido blando, pero principalmente em la piel, cavidad oral y el tracto digestivo. Se describe un caso de plasmocitoma anaplásico cutâneo em un perro, mestizo, de 16 años de edad. El animal mostro múltiples nódulos de color rosado, irregulares y de consistência blanda, extendidos em la piel. Em elexamen histopatológico se observo área circunscrita consistente en células redondas que muestran intensa anisocariosis, anisocitosis y número moderado de células gigantes, multinucleadas. La evaluación imunohistoquímica reveló expresión de CD79a y MUM1, lo que permite el diagnóstico de plasmocitoma anaplásico.(AU)

Plasmacytomas are uncommon neoplasms in dogs originating in the plasma cells.Occur in any soft tissue, but mainly in the skin, oral cavity and digestive tract. We reported a case of cutaneous anaplastic plasmocytoma in a dog, mixed breed, 16 years old. The animal showed multiple nodules of pinkish color, rough and smooth consistency, spread on the skin. In the histopathological examination revealed circumscribed area consisting of round cells showing intense anisocytosis, anisokaryosis and moderate number of giant cells, multinucleated. The immunohistochemical demonstrated expression for MUM1 and CD79a, allowing diagnosis of anaplastic plasmacytoma.(AU)

Plasmocitomas são neoplasias incomuns no cão, originárias dos plasmócitos. Ocorrem em qualquer tecido mole, mas principalmente na pele, cavidade oral e trato digestivo. Relata-se um caso de plasmocitoma anaplásico cutâneo em um cão, mestiço, de 16 anos. O animal apresentava vários nódulos de coloração rósea, irregulares e de consistência macia, distribuídos na pele. Na análise histopatológica observou-se área circunscrita constituída de células redondas exibindo intensa anisocitose, anisocariose e moderada quantidade de células gigantes, multinucleadas. A avaliação imunohistoquímica revelou expressão para MUM1 e CD79a, permitindo o diagnóstico de plasmocitoma anaplásico.(AU)

Evaluación imunohistoquímica de plasmocitoma cutáneo en un perro - reporte de un caso / Assessment immunohistochemistry of the cutaneous plasmacytoma in a dog - case report / Avaliação imunohistoquímica de plasmocitoma cutâneo em um cão: relato de caso

Los plasmacitomas son neoplasias poco comunes em los perros, procedentes de plasmocitos. Ocurre en cualquier tejido blando, pero principalmente em la piel, cavidad oral y el tracto digestivo. Se describe un caso de plasmocitoma anaplásico cutâneo em un perro, mestizo, de 16 años de edad. El animal mostro múltiples nódulos de color rosado, irregulares y de consistência blanda, extendidos em la piel. Em elexamen histopatológico se observo área circunscrita consistente en células redondas que muestran intensa anisocariosis, anisocitosis y número moderado de células gigantes, multinucleadas. La evaluación imunohistoquímica reveló expresión de CD79a y MUM1, lo que permite el diagnóstico de plasmocitoma anaplásico.

Plasmacytomas are uncommon neoplasms in dogs originating in the plasma cells.Occur in any soft tissue, but mainly in the skin, oral cavity and digestive tract. We reported a case of cutaneous anaplastic plasmocytoma in a dog, mixed breed, 16 years old. The animal showed multiple nodules of pinkish color, rough and smooth consistency, spread on the skin. In the histopathological examination revealed circumscribed area consisting of round cells showing intense anisocytosis, anisokaryosis and moderate number of giant cells, multinucleated. The immunohistochemical demonstrated expression for MUM1 and CD79a, allowing diagnosis of anaplastic plasmacytoma.

Plasmocitomas são neoplasias incomuns no cão, originárias dos plasmócitos. Ocorrem em qualquer tecido mole, mas principalmente na pele, cavidade oral e trato digestivo. Relata-se um caso de plasmocitoma anaplásico cutâneo em um cão, mestiço, de 16 anos. O animal apresentava vários nódulos de coloração rósea, irregulares e de consistência macia, distribuídos na pele. Na análise histopatológica observou-se área circunscrita constituída de células redondas exibindo intensa anisocitose, anisocariose e moderada quantidade de células gigantes, multinucleadas. A avaliação imunohistoquímica revelou expressão para MUM1 e CD79a, permitindo o diagnóstico de plasmocitoma anaplásico.

Immunohistochemistry in myocardium of dogs naturally infected with Leishmania chagasi / Imunohistoquímica em miocárdio de cães naturamente infectados por Leishmania chagasi

Background: Canine visceral leishmaniasis is a chronic and endemic disease, widespread zoonotic infection with a greatimpact on public health. The domestic dog is the main reservoir and also a source of infection for humans in the urbanenvironment. Canine visceral leishmaniasis is a multisystem disease that affects several organs, mainly the heart. The aimof this study was to verify the presence of amastigotes of Leishmania chagasi in myocardium of dogs naturally infectedby immunohistochemistry. In addition, to correlate the number of parasitized cells with the intensity of the inflammation,the macroscopic and microscopic lesions.Materials, Methods & Results: It were used 52 dogs positive for leishmaniasis diagnosed by clinical examination, IndirectImmunofluorescence (RIFI), ELISA and cytological analysis of popliteal lymph nodes. The positive animals were euthanized and submitted a necropsy. Following necropsy, the macroscopic analysis was done, and samples from the region ofthe cardiac apex were collected at the transition between the two ventricles. Fragments of heart tissues were fixed in 10%neutral formalin for histopathological and immunohistochemistry analysis. The inflammatory process of the myocardiumand lesions semi-quantitatively scored for myocardial injury on a scale of 0 to 3: (0) absent, (1) mild = small focal lesions,(2) moderate = larger, multifocal lesions and (3) severe = affecting most areas with coalescence. On immunostaining,the animal was classified according to the parasite load: - (no microorganism), mild (1-100 microorganisms), moderate(101-300), intense parasitism (> 300). At the macroscopically analysis, 33 animals (63.46%) had at least one lesion. Theconcentric hypertrophy left ventricular was the most...(AU)

Immunohistochemistry in myocardium of dogs naturally infected with Leishmania chagasi / Imunohistoquímica em miocárdio de cães naturamente infectados por Leishmania chagasi

Background: Canine visceral leishmaniasis is a chronic and endemic disease, widespread zoonotic infection with a greatimpact on public health. The domestic dog is the main reservoir and also a source of infection for humans in the urbanenvironment. Canine visceral leishmaniasis is a multisystem disease that affects several organs, mainly the heart. The aimof this study was to verify the presence of amastigotes of Leishmania chagasi in myocardium of dogs naturally infectedby immunohistochemistry. In addition, to correlate the number of parasitized cells with the intensity of the inflammation,the macroscopic and microscopic lesions.Materials, Methods & Results: It were used 52 dogs positive for leishmaniasis diagnosed by clinical examination, IndirectImmunofluorescence (RIFI), ELISA and cytological analysis of popliteal lymph nodes. The positive animals were euthanized and submitted a necropsy. Following necropsy, the macroscopic analysis was done, and samples from the region ofthe cardiac apex were collected at the transition between the two ventricles. Fragments of heart tissues were fixed in 10%neutral formalin for histopathological and immunohistochemistry analysis. The inflammatory process of the myocardiumand lesions semi-quantitatively scored for myocardial injury on a scale of 0 to 3: (0) absent, (1) mild = small focal lesions,(2) moderate = larger, multifocal lesions and (3) severe = affecting most areas with coalescence. On immunostaining,the animal was classified according to the parasite load: - (no microorganism), mild (1-100 microorganisms), moderate(101-300), intense parasitism (> 300). At the macroscopically analysis, 33 animals (63.46%) had at least one lesion. Theconcentric hypertrophy left ventricular was the most...