Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene

This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene.

Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene

This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene.(AU)

Mensurações ultrassonográficas da cisterna da glândula mamária de caprino transgênico / Ultrasound measurements of the mammary gland of transgenic hormone-induced lactating goat

Milk production of transgenic does was evaluated by ultrasound measurements of the mammary gland. Two Canindé goats, which were nine months of age were used in the trial, one non-transgenic or other transgenic for hG-CSF. For hormone-induced lactation, animals were given estradiol (0.25mg/kg, IM), progesterone (0.75mg/kg, IM), and prednisolone (0.4mg/kg, IM). Ultrasonographic exams were carried out during milking, using a Falcon 100 ultrasound equipment with a 5MHz convex probe and were performed by the same operator. The results were expressed as mean±standard error. The maximum greater length and shorter length of the cistern were respectively 5.14cm and 1.36cm for the transgenic animal and 7.28cm and 2.25cm for non-transgenic, which is consistent with the maximum milk volume produced. The relationship between the average area of cisterns and milk yield was expressed as a linear correlation curve, with a correlation coefficient significantly positive for both transgenic (Y=-1.1314+10.8538*x; r=0.97) and non-transgenic (Y=-21.7551+18.3634*x; r=0.97) animals. In conclusion, the ultrasound is a practice and appropriate technique to evaluate the cisterns in ruminant udders in transgenic animal.(AU)

Oocytoe production and in vitro maturation in Canindé goats following hormonal ovarian stimulation

This study e valuated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus - oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro matur ation (IVM) . Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg m edroxyprogesterone acetate for 11 days, together with a n injection of 50 μg d - cloprostenol on the 8 th day , along with additional treatment re g imens , as follows : i) five doses (5D), 120 mg of NIH - FSH - P1 in five injections at 12 h inter vals; ii) three doses (3D), 120 mg of NIH - FSH - P1 in three injections at 24 h i nterv als; iii) single dose (1D), 70 mg of NIH - FSH - P 1 and 200 IU of eCG at 36 h prior to sponge rem oval. Three sessions of hormonal treatment/oocyte recover y w ere performed and goats ( n = 6 / group) were allocated to different grou ps . The oocytes were collect ed by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being rec overed , resulting in a recovery rate of 74.7 % ( 577/772 ) . The mean number of CO Cs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). R ecovery rate was higher (P < 0.05) in the 5 D group ( 84.1 % ; 211/251 ) compared to the 3 D ( 68.2 % ; 182/267 ) and 1D group s (72.4% ; 184/254 ). T he lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1% ; 27/84 ), while the rate for the 5D and 1D groups was 49.1% ( 53/108 ) and 46.2% ( 42/91 ) , respectively. Finally, taking into account the main performance results of th e three treatments, it i s advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes .

Oocytoe production and in vitro maturation in Canindé goats following hormonal ovarian stimulation

This study e valuated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus - oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro matur ation (IVM) . Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg m edroxyprogesterone acetate for 11 days, together with a n injection of 50 μg d - cloprostenol on the 8 th day , along with additional treatment re g imens , as follows : i) five doses (5D), 120 mg of NIH - FSH - P1 in five injections at 12 h inter vals; ii) three doses (3D), 120 mg of NIH - FSH - P1 in three injections at 24 h i nterv als; iii) single dose (1D), 70 mg of NIH - FSH - P 1 and 200 IU of eCG at 36 h prior to sponge rem oval. Three sessions of hormonal treatment/oocyte recover y w ere performed and goats ( n = 6 / group) were allocated to different grou ps . The oocytes were collect ed by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being rec overed , resulting in a recovery rate of 74.7 % ( 577/772 ) . The mean number of CO Cs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). R ecovery rate was higher (P < 0.05) in the 5 D group ( 84.1 % ; 211/251 ) compared to the 3 D ( 68.2 % ; 182/267 ) and 1D group s (72.4% ; 184/254 ). T he lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1% ; 27/84 ), while the rate for the 5D and 1D groups was 49.1% ( 53/108 ) and 46.2% ( 42/91 ) , respectively. Finally, taking into account the main performance results of th e three treatments, it i s advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes .(AU)