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1.
Ciênc. anim. bras. (Impr.) ; 24: e-74695E, 2023. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1447905

Resumo

To assist the reproductive management of tambaqui (Colossoma macropomum) males in laboratory and commercial fish farming, a linear regression model was obtained from concentration curves using the spectrophotometric method. Twenty-two tambaqui males with an average age of three years old were selected and divided into two groups containing 11 animals each. Both groups alternately received a single dose of carp pituitary extract (CPE; 2.0 mg/kg body weight, intracoelomic). Sperm was collected 14 h after hormonal treatment and diluted (1:4000; sperm:formaldehyde saline). The concentration was estimated by counting spermatozoa in a Neubauer chamber and by using a spectrophotometer (λ=540 nm). Individual sperm concentration ranged from 11.40 to 71.13 × 109 sperm/mL. The degree of transmittance ranged from 62.1% to 95.0%. There was a significant correlation (r2 = 0.966; p < 0.0001) between sperm concentration analyzed in a Neubauer chamber and transmittance at 540 nm. Analysis by spectrophotometry and the prediction provided by the equation Y=100.293 - 0.509X proved to be an efficient and fast method for estimating sperm concentration in tambaqui and can be used in routine procedures in artificial fish reproduction laboratories.


Visando auxiliar o manejo reprodutivo de machos de tambaqui (Colossoma macropomum) em piscicultura de laboratório e comercial, obteve-se um modelo de regressão linear a partir de curvas de concentração por método espectrofotométrico. Foram selecionados 22 machos de tambaqui com idade média de três anos. Eles foram divididos em dois grupos contendo 11 animais cada. Ambos os grupos receberam alternadamente uma única dose de extrato de hipófise de carpa (EHC; 2,0 mg/kg de peso corporal, intracelomático). O esperma foi coletado 14 horas após o tratamento hormonal e diluído (1:4000; esperma: solução salina formaldeído). A concentração foi estimada por contagem de espermatozoides em câmara de Neubauer e por espectrofotômetro (λ=540 nm). A concentração espermática individual variou de 11,40 a 71,13 × 109 espermatozoides/mL. O grau de transmitância variou de 62,1 a 95,0%. Houve correlação significativa (r2 = 0,966; p < 0,0001) entre a concentração espermática analisada em câmara de Neubauer e a transmitância em 540 nm. A análise por espectrofotometria e a predição pela equação Y=100,293 - 0,509X mostrou ser um método eficiente e rápido para estimar a concentração espermática de tambaqui, podendo ser utilizado em procedimentos de rotina em laboratórios de reprodução artificial de peixes.


Assuntos
Animais , Reprodução , Sêmen , Peixes/fisiologia , Modelos Lineares
2.
Anim. Reprod. (Online) ; 19(1): e20210083, 2022. tab, graf
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1360099

Resumo

Abstract The action of substances with non-permeable cryoprotectant potential, besides glucose, has not yet been studied for the species Prochilodus brevis. The objective of this work was to evaluate the action of four non-permeable cryoprotectants on this species sperm cryopreservation. Five pools were cryopreserved in a solution of 5% glucose and 10% dimethyl sulfoxide (Me2SO) associated or not (control) with cryoprotectants egg yolk (5, 10 or 12%), soy lecithin (2.5, 7.5 or 10%), sucrose (5, 10 or 20%) and lactose (5, 8 or 15%). After thawing, samples were evaluated for sperm kinetics (total motility, motility duration, velocities, and wobble - WOB), morphology and membrane and DNA integrity. The treatments containing egg yolk improved significantly (P<0.05) results when compared the control for the membrane integrity parameter. When compared to other treatments, egg yolk, at any concentration, presented higher results (P<0.05) for membrane integrity, total motility, curvilinear velocity (VCL) and average path velocity (VAP) parameters. Egg yolk also showed the best results for WOB, but it did not differ from 5% and 8% lactose and 5% and 20% sucrose. Soy lecithin had the lowest percentages of morphologically normal sperm (P<0.05), while the other treatments did not differ from each other. There was no difference regarding DNA integrity data. Thus, 5% egg yolk is indicated as a non-permeable cryoprotectant for P. brevis, in association with 5% glucose and 10% Me2SO.

3.
Anim. Reprod. ; 19(1): e20210083, 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-765785

Resumo

The action of substances with non-permeable cryoprotectant potential, besides glucose, has not yet been studied for the species Prochilodus brevis. The objective of this work was to evaluate the action of four non-permeable cryoprotectants on this species sperm cryopreservation. Five pools were cryopreserved in a solution of 5% glucose and 10% dimethyl sulfoxide (Me2SO) associated or not (control) with cryoprotectants egg yolk (5, 10 or 12%), soy lecithin (2.5, 7.5 or 10%), sucrose (5, 10 or 20%) and lactose (5, 8 or 15%). After thawing, samples were evaluated for sperm kinetics (total motility, motility duration, velocities, and wobble - WOB), morphology and membrane and DNA integrity. The treatments containing egg yolk improved significantly (P<0.05) results when compared the control for the membrane integrity parameter. When compared to other treatments, egg yolk, at any concentration, presented higher results (P<0.05) for membrane integrity, total motility, curvilinear velocity (VCL) and average path velocity (VAP) parameters. Egg yolk also showed the best results for WOB, but it did not differ from 5% and 8% lactose and 5% and 20% sucrose. Soy lecithin had the lowest percentages of morphologically normal sperm (P<0.05), while the other treatments did not differ from each other. There was no difference regarding DNA integrity data. Thus, 5% egg yolk is indicated as a non-permeable cryoprotectant for P. brevis, in association with 5% glucose and 10% Me2SO.(AU)


Assuntos
Animais , Peixes , Criopreservação , Characidae , Crioprotetores
4.
Ciênc. Anim. (Impr.) ; 30(04, Supl. 2): 337-341, 2020. tab
Artigo em Português | VETINDEX | ID: biblio-1472590

Resumo

Prochilodus brevis is a fish species with an important ecological and economic role. Therefore, its captive breeding, together with sperm cryopreservation, come as an alternative to improve its availability. To perform sperm cryopreservation with quality results, it is necessary to develop studies on cryomedia composition, such as non-permeable cryoprotectants. The aim of the study was to test the efficacy of two non-permeable cryoprotectants on post-thawed kinetic parameters of Prochilodus brevis sperm. Twenty reproductive mature P. brevis males were selected and induced to sperm with carp pituitary extract. The sperm was collected, and five pools were made. Pools were diluted in 5% glucose and 10% DMSO added or not with three different concentrations of egg yolk (5; 10 or 12%). The samples were placed on French straws, frozen in dry shipper and stored in liquid nitrogen. After thawing, the kinetic analyses of computer assisted sperm analysis (CASA) were performed. There were no significant differences between the total motility of the control (60.48%) and treatments (49.0; 47.8 and 51.68%, respectively). The other kinetic parameters also showed no statistical difference. Thus, there is no need to use egg yolk in the freezing of P. brevis sperm when using a glucose and DMSO extender.


Assuntos
Masculino , Animais , Criopreservação/métodos , Criopreservação/veterinária , Gema de Ovo/efeitos adversos , Peixes
5.
Ci. Anim. ; 30(04, Supl. 2): 337-341, 2020. tab
Artigo em Português | VETINDEX | ID: vti-32193

Resumo

Prochilodus brevis is a fish species with an important ecological and economic role. Therefore, its captive breeding, together with sperm cryopreservation, come as an alternative to improve its availability. To perform sperm cryopreservation with quality results, it is necessary to develop studies on cryomedia composition, such as non-permeable cryoprotectants. The aim of the study was to test the efficacy of two non-permeable cryoprotectants on post-thawed kinetic parameters of Prochilodus brevis sperm. Twenty reproductive mature P. brevis males were selected and induced to sperm with carp pituitary extract. The sperm was collected, and five pools were made. Pools were diluted in 5% glucose and 10% DMSO added or not with three different concentrations of egg yolk (5; 10 or 12%). The samples were placed on French straws, frozen in dry shipper and stored in liquid nitrogen. After thawing, the kinetic analyses of computer assisted sperm analysis (CASA) were performed. There were no significant differences between the total motility of the control (60.48%) and treatments (49.0; 47.8 and 51.68%, respectively). The other kinetic parameters also showed no statistical difference. Thus, there is no need to use egg yolk in the freezing of P. brevis sperm when using a glucose and DMSO extender.(AU)


Assuntos
Animais , Masculino , Criopreservação/métodos , Criopreservação/veterinária , Gema de Ovo/efeitos adversos , Peixes
6.
R. bras. Reprod. Anim. ; 39(1): 184-188, jan. -mar. 2015.
Artigo em Português | VETINDEX | ID: vti-12849

Resumo

Objetivou-se com este manuscrito realizar uma breve revisão sobre técnicas de reprodução assistida utilizadas para peixes, apresentando uma visão geral sobre a conservação de gametas e embriões e os avanços tecnológicos já alcançados. Contextualizando com sua importância para a preservação de material genética e aplicação comercial.(AU)


The aim of this manuscript was to conduct a brief review about assisted reproduction techniques used to fish, presenting an overview of the gametes and embryos conservation and technological advances already achieved in this field. Contextualizing to their importance for the preservation of genetic material and commercial application.(AU)


Assuntos
Animais , Peixes/embriologia , Embrião não Mamífero/embriologia , Criopreservação/normas , Criopreservação/veterinária , Técnicas de Reprodução Assistida
7.
Rev. bras. reprod. anim ; 39(1): 184-188, jan. -mar. 2015.
Artigo em Português | VETINDEX | ID: biblio-1492162

Resumo

Objetivou-se com este manuscrito realizar uma breve revisão sobre técnicas de reprodução assistida utilizadas para peixes, apresentando uma visão geral sobre a conservação de gametas e embriões e os avanços tecnológicos já alcançados. Contextualizando com sua importância para a preservação de material genética e aplicação comercial.


The aim of this manuscript was to conduct a brief review about assisted reproduction techniques used to fish, presenting an overview of the gametes and embryos conservation and technological advances already achieved in this field. Contextualizing to their importance for the preservation of genetic material and commercial application.


Assuntos
Animais , Criopreservação/normas , Criopreservação/veterinária , Embrião não Mamífero/embriologia , Peixes/embriologia , Técnicas de Reprodução Assistida
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