Resumo
The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%), enrofloxacin (57.31%), gentamicin (39.02%), neomycin (37.8%), apramycin (30.48%), colistine (30.48%) and cefalexin (6.09%). A number of 32 (39.02%) E. coli isolates harbored plasmids.
O presente estudo teve por objetivo determinar os padrões moleculares e de resistência aos antimicrobianos de isolados de E. coli provenientes do trato urinário de suínos no Sul do Brasil. Os fatores estudados dividiram os patotipos ETEC, STEC e UPEC. Trinta e quatro (38,63%) isolados avaliados apresentavam um ou mais dos fatores de virulência pesquisados. A freqüência dos genes de virulência detectados foram: pap (10,97%), hlyA (10,97%), iha (9,75%), lt (8,53%), sta (7,31%) sfa (6,09%), f4 (4,87%), f5 (4,87%), stb (4,87%), f6 (1,21%) e f41 (1,21%). Os isolados foram resistentes à penicilina (95,12%), lincomicina (93,9%), eritromicina (92,68%), tetraciclina (90,24%), amoxacilina (82,92%), ampicilina (74,39%), josamicina (79,26%), norfloxacina (58,53%), enrofloxacina (57,31%), gentamicina (39,02%), neomicina (37,8%), apramicina (30,48%), colistina (30,48%) e cefalexina (6,09%). Trinta e dois (39,02%) isolados de E. coli continham plasmídeos.