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1.
R. bras. Ci. avíc. ; 21(2): eRBCA-2018-0954, nov. 2019. ilus, graf, tab
Artigo em Inglês | VETINDEX | ID: vti-26237

Resumo

This study conducted an in-depth investigation on the development of GABAergic neurons and their receptors in HPG axis-related target organs of Wenchang chicks under heat stress. One-day-old healthy Wenchang chicks were randomly divided into control (CK) and heat stress (HS) groups. Chicks in the HS group were placed in a 40±0.5°C climatic chamber for HS treatment from 13:00 to 15:00 daily. By immunohistochemistry and Western blotting, GABA and GABAA receptor (GABAAR) expression in the hypothalamus of the HS group was significantly higher (p 0.05), but GABAB receptor (GABABR) expression was significantly lower than that of the CK group (p 0.05). Expression of GABA and its two receptors in the pituitary tissues of the HS group was significantly lower than in the CK group (p 0.05). Expression of GABA and GABABR in ovaries in the HS group was significantly higher, but expression of GABAAR in the testes of the HS group was lower than that of the CK group (p 0.05). In the male chicks, expression of GABA and its two receptors in the hypothalamus, pituitary, and testicular tissues of the HS group was significantly higher than that of the CK group (p 0.05). Western blotting showed that the GABAAR and GABABR expression of the HS group was significantly higher than that of the CK group at 3 and 5 weeks of age. Thus, HS caused GABAergic nervous system disorder in the HPG axis of Wenchang chicks and seriously hindered the normal development of GABAergic neurons in chicks, leading to the disorder of the expression of GABA and its receptors in tissues.(AU)


Assuntos
Animais , Galinhas/fisiologia , Resposta ao Choque Térmico , Neurônios , Hipotálamo , Hipófise , Hormônios Gonadais
2.
Rev. bras. ciênc. avic ; 21(2): eRBCA, 2019. ilus, graf, tab
Artigo em Inglês | VETINDEX | ID: biblio-1490646

Resumo

This study conducted an in-depth investigation on the development of GABAergic neurons and their receptors in HPG axis-related target organs of Wenchang chicks under heat stress. One-day-old healthy Wenchang chicks were randomly divided into control (CK) and heat stress (HS) groups. Chicks in the HS group were placed in a 40±0.5°C climatic chamber for HS treatment from 13:00 to 15:00 daily. By immunohistochemistry and Western blotting, GABA and GABAA receptor (GABAAR) expression in the hypothalamus of the HS group was significantly higher (p 0.05), but GABAB receptor (GABABR) expression was significantly lower than that of the CK group (p 0.05). Expression of GABA and its two receptors in the pituitary tissues of the HS group was significantly lower than in the CK group (p 0.05). Expression of GABA and GABABR in ovaries in the HS group was significantly higher, but expression of GABAAR in the testes of the HS group was lower than that of the CK group (p 0.05). In the male chicks, expression of GABA and its two receptors in the hypothalamus, pituitary, and testicular tissues of the HS group was significantly higher than that of the CK group (p 0.05). Western blotting showed that the GABAAR and GABABR expression of the HS group was significantly higher than that of the CK group at 3 and 5 weeks of age. Thus, HS caused GABAergic nervous system disorder in the HPG axis of Wenchang chicks and seriously hindered the normal development of GABAergic neurons in chicks, leading to the disorder of the expression of GABA and its receptors in tissues.


Assuntos
Animais , Galinhas/fisiologia , Neurônios , Resposta ao Choque Térmico , Hipotálamo , Hipófise , Hormônios Gonadais
3.
Rev. bras. ciênc. avic ; 18(2): 277-282, apr.-jun. 2016. graf
Artigo em Inglês | VETINDEX | ID: biblio-1490261

Resumo

We investigated the effect of heat stress (HS) on the expression of the GABA receptor in the hypothalamic-pituitary-gonadal (HPG) axis of Wenchang chickens. Real-time quantitative RT-PCR (qRT-PCR) was used to quantify the GABA receptor mRNA levels along the HPG axis of chickens under HS (40±0.5 °C) for 1-6 weeks. Our results showed that the expression of GABAA and GABAB receptor at the mRNAs levels in the tissues of HPG axis exhibited fluctuation and variability. After HS, the mRNA level of GABAA receptor was significantly reduced in the hypothalamus of 1-week-old and in the pituitary of 3-week-old chickens, but significantly increased in the pituitary of 1-, 4-, and 5-week-old chickens. The GABAB receptor mRNA level significantly declined in the hypothalamus of 1-week-old and in the pituitary of 3-week-old chickens, but was significantly upregulated in the pituitary and testis of 1- and 2-week-old chickens. At other time points, the expressions of GABAA receptor and GABAB receptor showed no significant differences compared with control group. These results indicated that the levels of GABAA receptor and GABAB receptor mRNAs varied in different tissues of the HPG axis in chickens of different ages, displaying temporal and spatial variations. GABA receptor behaved as a positively-regulated gene by HS, i.e., its mRNA was increased by HS; similarly, it was a negatively-regulated gene by HS, when its expression was reduced by HS.


Assuntos
Animais , Galinhas/anormalidades , Transtornos de Estresse por Calor/veterinária , RNA Mensageiro/análise , RNA Mensageiro/isolamento & purificação , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária
4.
R. bras. Ci. avíc. ; 18(2): 277-282, apr.-jun. 2016. graf
Artigo em Inglês | VETINDEX | ID: vti-338219

Resumo

We investigated the effect of heat stress (HS) on the expression of the GABA receptor in the hypothalamic-pituitary-gonadal (HPG) axis of Wenchang chickens. Real-time quantitative RT-PCR (qRT-PCR) was used to quantify the GABA receptor mRNA levels along the HPG axis of chickens under HS (40±0.5 °C) for 1-6 weeks. Our results showed that the expression of GABAA and GABAB receptor at the mRNAs levels in the tissues of HPG axis exhibited fluctuation and variability. After HS, the mRNA level of GABAA receptor was significantly reduced in the hypothalamus of 1-week-old and in the pituitary of 3-week-old chickens, but significantly increased in the pituitary of 1-, 4-, and 5-week-old chickens. The GABAB receptor mRNA level significantly declined in the hypothalamus of 1-week-old and in the pituitary of 3-week-old chickens, but was significantly upregulated in the pituitary and testis of 1- and 2-week-old chickens. At other time points, the expressions of GABAA receptor and GABAB receptor showed no significant differences compared with control group. These results indicated that the levels of GABAA receptor and GABAB receptor mRNAs varied in different tissues of the HPG axis in chickens of different ages, displaying temporal and spatial variations. GABA receptor behaved as a positively-regulated gene by HS, i.e., its mRNA was increased by HS; similarly, it was a negatively-regulated gene by HS, when its expression was reduced by HS.(AU)


Assuntos
Animais , Transtornos de Estresse por Calor/veterinária , Galinhas/anormalidades , RNA Mensageiro/análise , RNA Mensageiro/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase/veterinária
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