Resumo
Sporothrix schenckii is the etiological agent of the sporotrichosis in animals and humans being this mycosis of greatimportance in public health. Due to clinical, epidemiological and molecular differences described in other studies, this studyevaluated phenotypic and thermotolerance characteristics of 36 S. schenckii isolates from clinical cases of feline, canine andhuman sporotrichosis, four environmental isolates and two reference strains. Forty-two S. schenckii isolates from five townsof the south region of the Rio Grande do Sul, Brazil were utilized to phenotypic analyses, thermotolerance and conversion tothe yeast phase. Cultured isolates on lactrimel agar, Sabouraud dextrose agar added chloramphenicol and potato dextroseagar at 25 and 35oC showed differences in the colonies morphology and growth time (p=0,026) among reference strains and,clinical and environmental isolates. In the thermotolerance evaluation 26.2 % isolates were capable of growth at 41oC. Allisolates presented conversion to the yeast phase. Microscopic morphologies study showed statistical differences (p 0,01)among clinical isolates of felines and other species in relation to sessile pigmented and sympodial conidia. Our resultsdemonstrated morphological differences among S. schenckii clinical and environmental isolates of a same region and angreat probability of development of clinical forms disseminate
Resumo
RESUMO A dermatofitose é a micose cutânea de maior ocorrência na clínica de pequenos animais e, que pelo seu caráter zoonótico, é considerada uma importante doença em saúde pública. O presente trabalho teve como objetivo descrever um surto de dermatofitose neonatal canina causada por Microsporum gypseum. Sete filhotes com 20 dias de idade foram encaminhados para exame clinico onde cinco apresentavam áreas de alopecia, eritema e descamação no membro posterior e/ou cauda. A dermatofitose foi confirmada pelo isolamento de M. gypseum, sendo indicada terapia antifúngica tópica para todos os animais. Dois animais tiveram cura clínica espontânea das lesões e os outros foram tratados com cetoconazol ou miconazol por 30 dias, sendo obtida a negatividade da cultura fúngica ao final do tratamento. Palavras-chave: Dermatofitose, filhotes, antifúngico. CANINE NEONATAL DERMATOPHYTOSIS BY Microsporum gypseum ABSTRACT Dermatophytosis is a cutaneous mycosis of great occurrence in the small animal clinics and, that due to zoonotic character is considered an important disease in public health. This paper has the objective to report a outbreak of canine neonatal dermatophytosis caused by Microsporum gypseum. Seven puppies with 20 days old-age were submitted to clinical examination, where five showed regions of alopecia, erythema and scaling in the hindlimb and/or tail. Dermatophytosis
Resumo
RESUMO A dermatofitose é a micose cutânea de maior ocorrência na clínica de pequenos animais e, que pelo seu caráter zoonótico, é considerada uma importante doença em saúde pública. O presente trabalho teve como objetivo descrever um surto de dermatofitose neonatal canina causada por Microsporum gypseum. Sete filhotes com 20 dias de idade foram encaminhados para exame clinico onde cinco apresentavam áreas de alopecia, eritema e descamação no membro posterior e/ou cauda. A dermatofitose foi confirmada pelo isolamento de M. gypseum, sendo indicada terapia antifúngica tópica para todos os animais. Dois animais tiveram cura clínica espontânea das lesões e os outros foram tratados com cetoconazol ou miconazol por 30 dias, sendo obtida a negatividade da cultura fúngica ao final do tratamento. Palavras-chave: Dermatofitose, filhotes, antifúngico. CANINE NEONATAL DERMATOPHYTOSIS BY Microsporum gypseum ABSTRACT Dermatophytosis is a cutaneous mycosis of great occurrence in the small animal clinics and, that due to zoonotic character is considered an important disease in public health. This paper has the objective to report a outbreak of canine neonatal dermatophytosis caused by Microsporum gypseum. Seven puppies with 20 days old-age were submitted to clinical examination, where five showed regions of alopecia, erythema and scaling in the hindlimb and/or tail. Dermatophytosis
Resumo
Sporothrix schenckii is the etiological agent of the sporotrichosis in animals and humans being this mycosis of greatimportance in public health. Due to clinical, epidemiological and molecular differences described in other studies, this studyevaluated phenotypic and thermotolerance characteristics of 36 S. schenckii isolates from clinical cases of feline, canine andhuman sporotrichosis, four environmental isolates and two reference strains. Forty-two S. schenckii isolates from five townsof the south region of the Rio Grande do Sul, Brazil were utilized to phenotypic analyses, thermotolerance and conversion tothe yeast phase. Cultured isolates on lactrimel agar, Sabouraud dextrose agar added chloramphenicol and potato dextroseagar at 25 and 35oC showed differences in the colonies morphology and growth time (p=0,026) among reference strains and,clinical and environmental isolates. In the thermotolerance evaluation 26.2 % isolates were capable of growth at 41oC. Allisolates presented conversion to the yeast phase. Microscopic morphologies study showed statistical differences (p 0,01)among clinical isolates of felines and other species in relation to sessile pigmented and sympodial conidia. Our resultsdemonstrated morphological differences among S. schenckii clinical and environmental isolates of a same region and angreat probability of development of clinical forms disseminate