Resumo
Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34E12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3
Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34E12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3
Resumo
Background: Stromal Grastrointestinal Tumors (GIST) are rare mesenchymal neoplasms that affect the wall of the gastrointestinal tract (GIT). Histologically this tumor shows a spindle, epithelioid or mixed aspect and routine hematoxylin and eosin (HE) staining does not allow adequate differentiation from other mesenquimal neoplasms. Histochemical and imunohistochemical techniques are useful tools in this differentiation. The aim of this study was to report a case of canine GIST, emphasizing the use of histochemical and immunohistochemical techniques to differentiate it from other histomorfologically similar mesenchymal neoplasms.Case: A ten-year-old male Pinscher dog was submitted to surgical excision of a tumor in the pyloric region of stomach. The dog was euthanized 90 days after surgery. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE and Gomoris Tricrome (GT). For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. Cytokeratin (CK) AE1/AE3, c-KIT, vimentin, Ki-67, S-100 and Smooth Muscle Alfa Actin (SMA) expression were evaluated. For vimentin, CKAE1AE3, SMA, S100 and c-KIT antibodies, a semiquantitative method was used and neoplasms were classifi ed as negative (-), pos
Background: Stromal Grastrointestinal Tumors (GIST) are rare mesenchymal neoplasms that affect the wall of the gastrointestinal tract (GIT). Histologically this tumor shows a spindle, epithelioid or mixed aspect and routine hematoxylin and eosin (HE) staining does not allow adequate differentiation from other mesenquimal neoplasms. Histochemical and imunohistochemical techniques are useful tools in this differentiation. The aim of this study was to report a case of canine GIST, emphasizing the use of histochemical and immunohistochemical techniques to differentiate it from other histomorfologically similar mesenchymal neoplasms.Case: A ten-year-old male Pinscher dog was submitted to surgical excision of a tumor in the pyloric region of stomach. The dog was euthanized 90 days after surgery. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE and Gomoris Tricrome (GT). For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. Cytokeratin (CK) AE1/AE3, c-KIT, vimentin, Ki-67, S-100 and Smooth Muscle Alfa Actin (SMA) expression were evaluated. For vimentin, CKAE1AE3, SMA, S100 and c-KIT antibodies, a semiquantitative method was used and neoplasms were classifi ed as negative (-), pos
Resumo
Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34E12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3
Background: Sinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy.Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more firm tissues like cartilage and bone tissue. Tumor specimens were collected, fixed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34E12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3
Resumo
Background: Stromal Grastrointestinal Tumors (GIST) are rare mesenchymal neoplasms that affect the wall of the gastrointestinal tract (GIT). Histologically this tumor shows a spindle, epithelioid or mixed aspect and routine hematoxylin and eosin (HE) staining does not allow adequate differentiation from other mesenquimal neoplasms. Histochemical and imunohistochemical techniques are useful tools in this differentiation. The aim of this study was to report a case of canine GIST, emphasizing the use of histochemical and immunohistochemical techniques to differentiate it from other histomorfologically similar mesenchymal neoplasms.Case: A ten-year-old male Pinscher dog was submitted to surgical excision of a tumor in the pyloric region of stomach. The dog was euthanized 90 days after surgery. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE and Gomoris Tricrome (GT). For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. Cytokeratin (CK) AE1/AE3, c-KIT, vimentin, Ki-67, S-100 and Smooth Muscle Alfa Actin (SMA) expression were evaluated. For vimentin, CKAE1AE3, SMA, S100 and c-KIT antibodies, a semiquantitative method was used and neoplasms were classifi ed as negative (-), pos
Background: Stromal Grastrointestinal Tumors (GIST) are rare mesenchymal neoplasms that affect the wall of the gastrointestinal tract (GIT). Histologically this tumor shows a spindle, epithelioid or mixed aspect and routine hematoxylin and eosin (HE) staining does not allow adequate differentiation from other mesenquimal neoplasms. Histochemical and imunohistochemical techniques are useful tools in this differentiation. The aim of this study was to report a case of canine GIST, emphasizing the use of histochemical and immunohistochemical techniques to differentiate it from other histomorfologically similar mesenchymal neoplasms.Case: A ten-year-old male Pinscher dog was submitted to surgical excision of a tumor in the pyloric region of stomach. The dog was euthanized 90 days after surgery. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 µm histological sections were obtained and stained with HE and Gomoris Tricrome (GT). For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. Cytokeratin (CK) AE1/AE3, c-KIT, vimentin, Ki-67, S-100 and Smooth Muscle Alfa Actin (SMA) expression were evaluated. For vimentin, CKAE1AE3, SMA, S100 and c-KIT antibodies, a semiquantitative method was used and neoplasms were classifi ed as negative (-), pos