First detection of Trypanosoma vivax in cattle in the state of Bahia, Brazil, based on parasitological and molecular analyses / Primeira detecção de Trypanosoma vivax em bovinos no estado da Bahia, Brasil baseada em análises parasitológica e molecular

We report the first detection of Trypanosoma vivax in Bahia state based on blood smear and PCR analyses. A total of 623 bovine blood samples were collected over two years. Parasitological analysis by smear technique detected the presence of T. vivax in 0.3%, while molecular analysis by PCR showed a prevalence in 18.9% of the samples. This study demonstrated the higher sensitivity of molecular analysis in the diagnosis of hemoparasitosis caused by T. vivax in dairy cattle herds.

Relatamos a primeira detecção de T. vivax no estado da Bahia baseada em esfregaços sanguíneos e PCR. Foram coletadas 623 amostras de sangue de bovinos ao longo de dois anos. An análise dos esfregaços detectou a presença do T. vivax em 0,3% delas, enquanto a detecção molecular por PCR mostrou uma prevalência em 18,9%. Este estudo evidenciou a maior sensibilidade da análise molecular no diagnóstico da hemoparasitose causada pelo T. vivax, em rebanhos bovinos leiteiros.

First detection of Trypanosoma vivax in cattle in the state of Bahia, Brazil, based on parasitological and molecular analyses / Primeira detecção de Trypanosoma vivax em bovinos no estado da Bahia, Brasil baseada em análises parasitológica e molecular

We report the first detection of Trypanosoma vivax in Bahia state based on blood smear and PCR analyses. A total of 623 bovine blood samples were collected over two years. Parasitological analysis by smear technique detected the presence of T. vivax in 0.3%, while molecular analysis by PCR showed a prevalence in 18.9% of the samples. This study demonstrated the higher sensitivity of molecular analysis in the diagnosis of hemoparasitosis caused by T. vivax in dairy cattle herds.(AU)

Relatamos a primeira detecção de T. vivax no estado da Bahia baseada em esfregaços sanguíneos e PCR. Foram coletadas 623 amostras de sangue de bovinos ao longo de dois anos. An análise dos esfregaços detectou a presença do T. vivax em 0,3% delas, enquanto a detecção molecular por PCR mostrou uma prevalência em 18,9%. Este estudo evidenciou a maior sensibilidade da análise molecular no diagnóstico da hemoparasitose causada pelo T. vivax, em rebanhos bovinos leiteiros.(AU)

Morphometric Parameters of Erythrocytes in Juvenile Mandarin Ducks Aix galericulata

The morphometric parameters of erythrocytes in Mandarin ducks- Aix galericulata have never been subjected to study even though this species is common in aviculture. The aim of this study is to investigate the morphometric parameters of erythrocytes in juvenile Mandarin ducks. A total of 1500 erythrocytes obtained from thirty Mandarin ducks were analysed. The smears of the peripheral blood were stained using Diff-Quik® stain method, and the length and width of erythrocytes were measured using ImageJ program. The average length of the measured erythrocytes was 11.879 µm. The maximal measured length of the erythrocytes was 16.049 µm, and the minimal measured length was 5.668 µm. The average width of the measured erythrocytes was 6.160 µm. The maximal measured width of the erythrocytes was 10.115 µm and the minimal measured width was 3.750 µm. In conclusion, knowing morphometric parameters of Mandarin duck erythrocytes is the first step in evaluation process of erythrocytes functional state and health condition of an individual.(AU)

Morphometric Parameters of Erythrocytes in Juvenile Mandarin Ducks Aix galericulata

The morphometric parameters of erythrocytes in Mandarin ducks- Aix galericulata have never been subjected to study even though this species is common in aviculture. The aim of this study is to investigate the morphometric parameters of erythrocytes in juvenile Mandarin ducks. A total of 1500 erythrocytes obtained from thirty Mandarin ducks were analysed. The smears of the peripheral blood were stained using Diff-Quik® stain method, and the length and width of erythrocytes were measured using ImageJ program. The average length of the measured erythrocytes was 11.879 µm. The maximal measured length of the erythrocytes was 16.049 µm, and the minimal measured length was 5.668 µm. The average width of the measured erythrocytes was 6.160 µm. The maximal measured width of the erythrocytes was 10.115 µm and the minimal measured width was 3.750 µm. In conclusion, knowing morphometric parameters of Mandarin duck erythrocytes is the first step in evaluation process of erythrocytes functional state and health condition of an individual.

Canine heartworm disease in Porto Velho: first record, distribution map and occurrence of positive mosquitoes

The aim of this study was to make the first report on canine heartworm disease in the state of Rondônia and confirm its transmission in this state. Blood samples were randomly collected from 727 dogs in the city of Porto Velho. The samples were analyzed to search for microfilariae and circulating antigens, using three different techniques: optical microscopy on thick blood smears stained with Giemsa; immunochromatography; and PCR. Mosquitoes were collected inside and outside the homes of all the cases of positive dogs and were tested using PCR to search for DNA of Dirofilaria immitis. Ninety-three blood samples out of 727 (12.8%) were positive according to the immunoassay technique and none according to the thick smear method. Among the 93 positive dogs, 89 (95.7%) were born in Porto Velho. No difference in the frequency of infection was observed between dogs raised indoors and in the yard. PCR on the mosquitoes resulted in only one positive pool. This result shows that the transmission of canine heartworm disease is occurring in the city of Porto Velho and that there is moderate prevalence among the dogs. The techniques of immunochromatography and PCR were more effective for detecting canine heartworm than thick blood smears. The confirmation of canine heartworm disease transmission in Porto Velho places this disease in the ranking for differential diagnosis of pulmonary nodules in humans in Rondônia.

O objetivo deste estudo foi de registrar pela primeira vez a dirofilariose canina no estado de Rondônia e confirmar sua transmissão neste estado. Amostras de sangue de 727 cães foram colhidas aleatoriamente na cidade de Porto Velho. As amostras foram analisadas em busca de microfilárias e antígenos circulantes usando três técnicas diferentes: microscopia ótica de gota espessa corada com Giemsa e imunocromatografia de fluxo lateral e PCR. Mosquitos foram colhidos no domicilio e peridomicílio de todos os casos de cães positivos, estes mosquitos foram testados pela PCR na detecção de DNA de Dirofilaria immitis. Noventa e três das 727 amostras de sangue foram positivas na técnica de imunoensaio (12,8%). Nenhuma amostra foi positiva na gota espessa. Entre os 93 cães positivos, 89 (95,7%) foram nascidos em Porto Velho. Nenhuma diferença na frequencia de infecção foi observada entre cães criados dentro da casa ou no quintal. O PCR dos mosquitos resultou em apenas um pool positivo. Este resultado mostra que a transmissão de dirofilariose canina está ocorrendo na cidade de Porto Velho e a frequência que ocorre nos cães é considerada moderada. As técnicas de imunocromatografia e PCR são mais eficazes na detecção de dirofilariose comparadas a gota espessa. A confirmação de transmissão de dirofilariose canina em Porto Velho, coloca esta doença no ranking de diagnóstico diferencial de nódulos pulmonares em seres humanos em Rondônia.

Babesia spp. infection in dogs from rural areas of São Paulo State, Brazil

The status of Babesia spp. infection in dogs from rural areas of São Paulo State, Brazil was studied. For this, 150 animals were examined by blood smears and by PCR; the presence of tick infestation was also investigated. By the blood smear examination, 3 animals (2%) were detected positive and by PCR for Babesia spp. 12 (8%) were positive, with bands visualized in 450 bp. Rhipicephalus sanguineus or Amblyomma spp. were found on 36 (24%) of the 150 dogs. Amblyomma species found were A. cajennense (9/36-25%) and A. ovale (9/36-25%). It was not possible to correlate the presence of R. sanguineus and the infection with Babesia spp. The sequencing of four positive samples demonstrated close identity with B. canis vogeli already characterized in Brazil.

A presença de infecção por Babesia spp. em cães de áreas rurais do Estado de São Paulo, Brasil foi investigada. Para tanto, 150 cães foram examinados por técnicas parasitológicas de esfregaços sanguíneos e moleculares (PCR), e também, foi verificada a presença de carrapatos nestes animais. Pela análise de esfregaços sanguíneos, 3 (2%) dos cães estavam infectados, enquanto pela PCR, 12 (8%) dos animais foram positivos com bandas aproximadas de 450 pares de base (pb). Foram observados 36 (24%) cães infestados com Rhipicephalus sanguineus ou com Amblyomma spp. As espécies de Amblyomma observadas foram A. cajennense (25%) e A. ovale (25%). Não foi possível correlacionar a presença de R. sanguineus com a infecção por Babesia spp. O seqüenciamento de quatro amostras positivas demonstrou alta identidade com B. canis vogeli, já caracterizada no Brasil.

Ocorrência de babesiose canina no Município de Uberlândia, Minas Gerais / Incidence of canine babesiosis in Uberlândia, Minas Gerais / Incidencia de babesiosis canina en el municipalidad de Uberlândia, Minas Gerais

Babesia canis é um protozoário intraeritrocítico transmitido pelo carrapato Rhipicephalus sanguineus que acomete principalmente canídeos domésticos e silvestres, causando manifestações clínicas como anorexia, pirexia, hemoglobinúria, esplenomegalia, hemoglobinemia, anemia grave, podendo o animal recuperar-se lentamente ou morrer. O objetivo desta pesquisa foi determinar a ocorrência da doença em Uberlândia - MG e comparar os métodos de esfregaços sanguíneos de ponta de orelha e Reação de Imunofluorescência Indireta (RIFI) no diagnóstico desta enfermidade. Para realização deste trabalho, foram colhidos aleatoriamente soros de 143 cães atendidos no Hospital Veterinário da Universidade Federal de Uberlândia para exame de RIFI enquanto que 772 esfregaços sanguíneos de ponta de orelha foram feitos e corados pelo Giemsa para a pesquisa de hemoparasito. Após a padronização da RIFI, considerou-se positivo soros com diluição igual ou superior a 1:20 e como negativos soros com diluição inferior a 1:20. Dos soros testados, através de RIFI, 51,74% estavam positivos e 48,26% negativos. Dos esfregaços sanguíneos 2,72% eram positivos para babesiose, 87,83% negativos e 9,45% positivos para outros hemoparasitos. Através do teste Qui-quadrado observou-se diferença significativa, ao nível de significância 5%, entre os métodos comparados.(AU)

Babesia canis is an intra-erythrocyte protozoan transmitted by the tick Rhipicephalus sanguineus that infects especially domestic and wild canids. The most common clinical manifestations include anorexia, pyrexia, hemoglobinuria, splenomegaly, hemoglobinemia, and severe anemia, leading to mortality or even to slow recuperation. The aim of this study was to determine the prevalence of this disease in Uberlândia, MG, and to compare the blood smears from the tip of the ear exam and the indirect immunofluorescence (IFI) test as diagnostic methods used for this disease. Thus, sera from 143 dogs attended at the Veterinary Hospital of the Federal University of Uberlândia were collected to detect IgG anti-Babesia canis by IFI test, while 772 blood smears from the tip of the ear were performed and stained by Giemsa to search the intra-erythrocyte parasite. Sera with titer higher than 1:20 were considered as positive for the IFI test. For this test 51,74% were found positive and 48,26% negative. From the blood smears, 2,72% were Babesia-positive, 97,83% were Babesia-negative and 9,45% were positive to other erythrocyte parasites. By using the chi-square test, there was a significant difference (p<0,05) between the two studied diagnostic methods.(AU)

Babesia canis es un protozoario intraeritrocítico transmitido por la garrapata Rhipicephalus sanguineus que ataca principalmente cánidos domésticos y silvestres causando manifestaciones clínicas como anorexia, pirexia, hemoglobinuria, esplenomegalia, hemoglobinemia, anemia grave, lo que puede levar a la muerte o a una lenta recuperación. El objetivo de esta investigación fue determinar la prevalencia de la enfermedad en Uberlândia MG y comparar los métodos de ricción sanguínea de punta de oreja y el de Inmunofluorescendia Indirecta (RIFI) en el diagnóstico de esta enfermedad. Para la realización de este trabajo fueron recolectados aletoriamente sueros de 143 canes, atendidos en el Hospital Veterinario de la Universidad de Uberlândia, para el examen de IFI. Mientras que 772 fricciones sanguíneas de punta de oreja fueron hechas y erojecidas por el Giemsa para la pesquisa de hemoparásito. Después de la padronización de la RIFI, se consideró positivo sueros con dilución superior a 1:20 y RIFI presentaron 51,74% de positividad y 48,26 % de negatividad. De las fricciones sanguíneas 2,72 % eran positivas para babesose, 87,83% negativas y 9,45% de positivos para otros hemoparásitos. Através del test Qui-cuadrado se observó una diferencia significativa al nivel de significancia del 5% entre los métodos comparados.(AU)