Response of Rhizobacterial strains and organic amendments on chickpea growth / Resposta de cepas de rizobactérias e alterações orgânicas no crescimento do grão-de-bico

Plant Growth Promoting Rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and promote plant growth through a variety of mechanisms such as phosphate solubilization, phytohormones production, antifungal activity and also improve plant growth and yield. Field experiment was carried out to investigate the residual effect of organic amendments plus soil microbes along with integrated nutrient management. (PGPR) are important soil organism that promotes plant growth and yield root colonization is an example of a direct and indirect mechanism. The treatments included control, (inorganic fertilizer and no organic fertilization).Five bacterial strains were identified morphologically and biochemically screened from the rhizospheres of chickpea, lentil, barseem, mungbean, and sesame. The experiment was conducted at the Arid Zone Research Center in D.I.Khan (Pakistan). The majority of isolates resulted in significant increase in shoot length, root length, and dry matter production of Cicer arietinum seedlings' shoot and root. The experiment represented that isolates treated plots with rhizobium strain inoculation resulted in greater plant height (35.000 cm) and nodule count (38.00) No of pods per plant-1 (44.66) when compared to the control treatment, While (Mesorhizobium cicero) along with organic amendments showed significant response the greater root length (50 cm) was observed in T4 treatment. The Performance of rhizobial strains on chickpea germination in an arid environment was found to significantly increase crop germination percentage. This combination thus increases nitrogen and phosphorus uptake in inoculation treated plots. The study found that plots with inoculation treatments yielded significantly higher than non-treated plots Treatment with Mesorhizobium Cicero and compost resulted in a higher grain yield (8%) as compared to the control. The greater grain yield was observed in Treatment T4 (183.67).The result showed that use of PGPR have the potential to increase nutrient absorption from soil while improved growth of chickpea.(AU)

As rizobactérias promotoras de crescimento de plantas (PGPR) são bactérias benéficas que colonizam as raízes das plantas e promovem o crescimento das plantas através de uma variedade de mecanismos, como solubilização de fosfato, produção de fito-hormônios, atividade antifúngica e também melhoram o crescimento e o rendimento das plantas. O experimento de campo foi realizado para investigar o efeito residual de corretivos orgânicos mais micróbios do solo, juntamente com o manejo integrado de nutrientes. PGPR são importantes organismos do solo que promovem o crescimento das plantas e produzem a colonização de raízes, que é um exemplo de mecanismo direto e indireto. Os tratamentos incluíram controle (fertilizante inorgânico e sem adubação orgânica). Cinco cepas bacterianas foram identificadas morfologicamente e bioquimicamente selecionadas das rizosferas de grão-de-bico, lentilha, barseem, feijão-mungo e gergelim. O experimento foi conduzido no Arid Zone Research Center em D.I.Khan (Paquistão). A maioria dos isolados resultou em aumento significativo no comprimento da parte aérea, comprimento da raiz e produção de matéria seca da parte aérea e raiz das plântulas de Cicer arietinum. O experimento demonstrou que isolados de parcelas tratadas com inoculação de cepa de rizóbio resultaram em maior altura de planta (35.000 cm) e contagem de nódulos (38,00) n.º de vagens por planta-1 (44,66), quando comparado ao tratamento controle, enquanto (Mesorhizobium cicero), juntamente com as alterações orgânicas, apresentaram resposta significativa quanto maior o comprimento da raiz (50 cm) observado no Tratamento T4. O desempenho de linhagens de rizóbios na germinação do grão-de-bico em ambiente árido aumentou significativamente a porcentagem de germinação da cultura. Esta combinação aumenta assim a absorção de nitrogênio e fósforo nas parcelas tratadas com inoculação. O estudo constatou que as parcelas com tratamentos de inoculação produziram significativamente mais do que as parcelas não tratadas. O tratamento com Mesorhizobium cicero e composto resultou em maior produtividade de grãos (8%) em comparação com o controle. A maior produtividade de grãos foi observada no Tratamento T4 (183,67). O resultado mostrou que o uso de PGPR tem potencial para aumentar a absorção de nutrientes do solo enquanto melhora o crescimento do grão-de-bico.(AU)

Biochemical characterization for determination of genetic distances among different indigenous chickpea (Cicer arietinum L. ) varieties of north-west Pakistan / Caracterização bioquímica para determinação de distâncias genéticas entre diferentes variedades indígenas de grão-de-bico (Cicer arietinum L. ) do noroeste do Paquistão

Genetic distances among different chickpea varieties and evaluation of their free amino acid profiles were determined on the basis of Sodium dodecyle sulphate polyacrylamide gels electrophoresis (SDS-PAGE). Total soluble proteins were resolved on 10% SDS Polyacrylamide gel. Low variability in tested varieties was observed. Dendogram based on electrophoretic data clustered the genotypes into 2 groups. The results showed that the average protein content of all the varieties was 26.01% within the range 22.8% for Thal-2006 to 34.06% Sheenghar-2000 of dry seed weight. On the basis of total protein content Bittal-98, Dasht and Sheen Ghar-2000, Karak-3 and CM-98, Paidar -91 and Fakhr-e-Thal, C-44, Balaksar and KK-1showed similar concentrations for protein contents among each other but showed variation from the rest of the varieties. Different proteins were separated on the basis of changes in their molecular weights by means of Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Dasht, CM-98, and Sheen Ghar showed 100% similarity. Balaksar and Fakhr-e- Thal, KK-2 and Chattan and KC-98, KK-1 and Lawaghar were 100% similar among each other but showed variation from the rest of the accessions. The overall dendrogram showed high and low level of variation among the accessions. The concentration of free amino acids varied among the 16 chickpea varieties. A significant difference of both essential and non-essential amino acids was found among the chickpea cultivars. The total concentration of essential amino acid was recorded 40.81 g/100 g protein while non-essential was recorded 59.18343 g/100 g protein in the given cultivars. The highest concentration of essential amino acids was found in C-44 followed by KK-2, KK-1 and Fakhr E Tal while the lowest concentration was recorded in Cm-98, Paidar-91 and Sheen Ghar-2000 respectively. Cultivars TAL-2006, Chattan and Karak-3 showed maximum concentration of both essential and endogenous amino acids. In conclusion; for broadening the genetic pools in breeding programs or to search for exotic characters, for instance new disease resistance alleles, accession with low similarity coefficients (Lawaghar and Battal-98) may be utilized. Furthermore the information acquired from this study could be used to device a proficient breeding approach intended at improving nutritional as well as broadening the genetic base of this essential food crop of Pakistan.(AU)

As distâncias genéticas entre as diferentes variedades de grão-de-bico e a avaliação de seus perfis de aminoácidos livres foram determinadas com base na eletroforese em gel de poliacrilamida com dodecil sulfato de sódio (SDS-PAGE). As proteínas solúveis totais foram resolvidas em SDS-PAGE a 10%. Foi observada baixa variabilidade nas variedades testadas. O dendrograma fundamentado em dados eletroforéticos agrupou os genótipos em dois grupos. Os resultados mostraram que o teor médio de proteínas de todas as variedades foi de 26,01%, na faixa de 22,8% para Thal-2006 a 34,06% para Sheenghar-2000 do peso de sementes secas. Com base no conteúdo total de proteínas, Bittal-98, Dasht, Sheen Ghar-2000, Karak-3, CM-98, Paidar-91, Fakhr-e-Thal, C-44, Balaksar e KK-1 apresentaram concentrações semelhantes para o conteúdo de proteínas entre si, mas tiveram variação quanto ao restante das variedades. Diferentes proteínas foram separadas com base nas alterações de seus pesos moleculares por meio de eletroforese em gel de poliacrilamida com dodecil sulfato de sódio (SDS-PAGE). Dasht, CM-98 e Sheen Ghar mostraram 100% de similaridade. Balaksar, Fakhr-e-Thal, KK-2, Chattan e KC-98, KK-1 e Lawaghar foram 100% semelhantes entre si, mas apresentaram variação em relação ao restante dos acessos. O dendrograma geral mostrou alto e baixo nível de variação entre os acessos. A concentração de aminoácidos livres variou entre as 16 variedades de grão-de-bico. Foi encontrada uma diferença significativa entre os aminoácidos essenciais e não essenciais nas cultivares de grão-de-bico. A concentração total de aminoácidos essenciais foi registrada em 40,81 g / 100 g de proteína, enquanto a não essencial foi registrada em 59,18343 g / 100 g de proteína nas cultivares. A maior concentração de aminoácidos essenciais foi encontrada em C-44, seguida de KK-2, KK-1 e Fakhr-e-Thal, enquanto a menor concentração foi registrada em CM-98, Paidar-91 e Sheen Ghar-2000. As cultivares TAL-2006, Chattan e Karak-3 apresentaram concentração máxima de aminoácidos essenciais e endógenos. Em conclusão, para ampliar os pools genéticos em programas de melhoramento ou procurar caracteres exóticos, por exemplo, novos alelos de resistência a doenças, pode ser utilizada a adesão com baixos coeficientes de similaridade (Lawaghar e Battal-98). Além disso, as informações adquiridas neste estudo poderiam ser usadas para criar uma abordagem de criação eficiente, com o objetivo de melhorar a nutrição e ampliar a base genética dessa cultura alimentar essencial do Paquistão.(AU)

Biochemical characterization for determination of genetic distances among different indigenous chickpea (Cicer arietinum L.) varieties of North-West Pakistan

Abstract Genetic distances among different chickpea varieties and evaluation of their free amino acid profiles were determined on the basis of Sodium dodecyle sulphate polyacrylamide gels electrophoresis (SDS-PAGE). Total soluble proteins were resolved on 10% SDS Polyacrylamide gel. Low variability in tested varieties was observed. Dendogram based on electrophoretic data clustered the genotypes into 2 groups. The results showed that the average protein content of all the varieties was 26.01% within the range 22.8% for Thal-2006 to 34.06% Sheenghar-2000 of dry seed weight. On the basis of total protein content Bittal-98, Dasht and Sheen Ghar-2000, Karak-3 and CM-98, Paidar -91 and Fakhr-e-Thal, C-44, Balaksar and KK-1showed similar concentrations for protein contents among each other but showed variation from the rest of the varieties. Different proteins were separated on the basis of changes in their molecular weights by means of Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Dasht, CM-98, and Sheen Ghar showed 100% similarity. Balaksar and Fakhr-e- Thal, KK-2 and Chattan and KC-98, KK-1 and Lawaghar were 100% similar among each other but showed variation from the rest of the accessions. The overall dendrogram showed high and low level of variation among the accessions. The concentration of free amino acids varied among the 16 chickpea varieties. A significant difference of both essential and non-essential amino acids was found among the chickpea cultivars. The total concentration of essential amino acid was recorded 40.81 g/100 g protein while non-essential was recorded 59.18343 g/100 g protein in the given cultivars. The highest concentration of essential amino acids was found in C-44 followed by KK-2, KK-1 and Fakhr E Tal while the lowest concentration was recorded in Cm-98, Paidar-91 and Sheen Ghar-2000 respectively. Cultivars TAL-2006, Chattan and Karak-3 showed maximum concentration of both essential and endogenous amino acids. In conclusion; for broadening the genetic pools in breeding programs or to search for exotic characters, for instance new disease resistance alleles, accession with low similarity coefficients (Lawaghar and Battal-98) may be utilized. Furthermore the information acquired from this study could be used to device a proficient breeding approach intended at improving nutritional as well as broadening the genetic base of this essential food crop of Pakistan.

Resumo As distâncias genéticas entre as diferentes variedades de grão-de-bico e a avaliação de seus perfis de aminoácidos livres foram determinadas com base na eletroforese em gel de poliacrilamida com dodecil sulfato de sódio (SDS-PAGE). As proteínas solúveis totais foram resolvidas em SDS-PAGE a 10%. Foi observada baixa variabilidade nas variedades testadas. O dendrograma fundamentado em dados eletroforéticos agrupou os genótipos em dois grupos. Os resultados mostraram que o teor médio de proteínas de todas as variedades foi de 26,01%, na faixa de 22,8% para Thal-2006 a 34,06% para Sheenghar-2000 do peso de sementes secas. Com base no conteúdo total de proteínas, Bittal-98, Dasht, Sheen Ghar-2000, Karak-3, CM-98, Paidar-91, Fakhr-e-Thal, C-44, Balaksar e KK-1 apresentaram concentrações semelhantes para o conteúdo de proteínas entre si, mas tiveram variação quanto ao restante das variedades. Diferentes proteínas foram separadas com base nas alterações de seus pesos moleculares por meio de eletroforese em gel de poliacrilamida com dodecil sulfato de sódio (SDS-PAGE). Dasht, CM-98 e Sheen Ghar mostraram 100% de similaridade. Balaksar, Fakhr-e-Thal, KK-2, Chattan e KC-98, KK-1 e Lawaghar foram 100% semelhantes entre si, mas apresentaram variação em relação ao restante dos acessos. O dendrograma geral mostrou alto e baixo nível de variação entre os acessos. A concentração de aminoácidos livres variou entre as 16 variedades de grão-de-bico. Foi encontrada uma diferença significativa entre os aminoácidos essenciais e não essenciais nas cultivares de grão-de-bico. A concentração total de aminoácidos essenciais foi registrada em 40,81 g / 100 g de proteína, enquanto a não essencial foi registrada em 59,18343 g / 100 g de proteína nas cultivares. A maior concentração de aminoácidos essenciais foi encontrada em C-44, seguida de KK-2, KK-1 e Fakhr-e-Thal, enquanto a menor concentração foi registrada em CM-98, Paidar-91 e Sheen Ghar-2000. As cultivares TAL-2006, Chattan e Karak-3 apresentaram concentração máxima de aminoácidos essenciais e endógenos. Em conclusão, para ampliar os pools genéticos em programas de melhoramento ou procurar caracteres exóticos, por exemplo, novos alelos de resistência a doenças, pode ser utilizada a adesão com baixos coeficientes de similaridade (Lawaghar e Battal-98). Além disso, as informações adquiridas neste estudo poderiam ser usadas para criar uma abordagem de criação eficiente, com o objetivo de melhorar a nutrição e ampliar a base genética dessa cultura alimentar essencial do Paquistão.

Potential for introducing improved production practices in food legumes with increased food security in Afghanistan

The restricted maximum likelihood method was used to assess performance following the introduction of improved varieties of chickpea and mungbean (an important source of plant protein in Afghanistan) as compared to local varieties using 242 farmer participatory demonstrations laid out in eight districts in Baghlan, Balkh and Uruzgan provinces in Afghanistan from 2009 to 2012. The impact of the varieties introduced on the enhancement of security of food and nutrition of farmers adopting such technologies was also assessed. Taking an average over the study period, chickpea improved varieties (Madad and Sehat) recorded 56 and 72 % more yield over the local ones, respectively, while in case of mungbean varieties, Mai 2008 and Maash 2008 recorded 22 and 30 % more yield over local ones respectively. Though there is a significant yield difference between the improved and the local varieties of both crops, the difference between the improved varieties of chickpea was not significant while it was significant in the case of mungbean. The study revealed a non-zero variance component for variety type [improved vs. local] × year within district interaction for the yield of chickpea while none of the interactions in mungbean had a positive variance component. Risk analysis showed that at a chosen probability level of 90 %, the improved varieties yielded more than local varieties in both crops (> 1.0 t ha1). Thus, the study highlighted the scope for enhancing the security of both food and nutrition in Afghanistan through improved productivity of pulse crops.

Potential for introducing improved production practices in food legumes with increased food security in Afghanistan

The restricted maximum likelihood method was used to assess performance following the introduction of improved varieties of chickpea and mungbean (an important source of plant protein in Afghanistan) as compared to local varieties using 242 farmer participatory demonstrations laid out in eight districts in Baghlan, Balkh and Uruzgan provinces in Afghanistan from 2009 to 2012. The impact of the varieties introduced on the enhancement of security of food and nutrition of farmers adopting such technologies was also assessed. Taking an average over the study period, chickpea improved varieties (Madad and Sehat) recorded 56 and 72 % more yield over the local ones, respectively, while in case of mungbean varieties, Mai 2008 and Maash 2008 recorded 22 and 30 % more yield over local ones respectively. Though there is a significant yield difference between the improved and the local varieties of both crops, the difference between the improved varieties of chickpea was not significant while it was significant in the case of mungbean. The study revealed a non-zero variance component for variety type [improved vs. local] × year within district interaction for the yield of chickpea while none of the interactions in mungbean had a positive variance component. Risk analysis showed that at a chosen probability level of 90 %, the improved varieties yielded more than local varieties in both crops (> 1.0 t ha1). Thus, the study highlighted the scope for enhancing the security of both food and nutrition in Afghanistan through improved productivity of pulse crops.(AU)

Biochemical characterization for determination of genetic distances among different indigenous chickpea (Cicer arietinum L.) varieties of North-West Pakistan

Abstract Genetic distances among different chickpea varieties and evaluation of their free amino acid profiles were determined on the basis of Sodium dodecyle sulphate polyacrylamide gels electrophoresis (SDS-PAGE). Total soluble proteins were resolved on 10% SDS Polyacrylamide gel. Low variability in tested varieties was observed. Dendogram based on electrophoretic data clustered the genotypes into 2 groups. The results showed that the average protein content of all the varieties was 26.01% within the range 22.8% for Thal-2006 to 34.06% Sheenghar-2000 of dry seed weight. On the basis of total protein content Bittal-98, Dasht and Sheen Ghar-2000, Karak-3 and CM-98, Paidar -91 and Fakhr-e-Thal, C-44, Balaksar and KK-1showed similar concentrations for protein contents among each other but showed variation from the rest of the varieties. Different proteins were separated on the basis of changes in their molecular weights by means of Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Dasht, CM-98, and Sheen Ghar showed 100% similarity. Balaksar and Fakhr-e- Thal, KK-2 and Chattan and KC-98, KK-1 and Lawaghar were 100% similar among each other but showed variation from the rest of the accessions. The overall dendrogram showed high and low level of variation among the accessions. The concentration of free amino acids varied among the 16 chickpea varieties. A significant difference of both essential and non-essential amino acids was found among the chickpea cultivars. The total concentration of essential amino acid was recorded 40.81 g/100 g protein while non-essential was recorded 59.18343 g/100 g protein in the given cultivars. The highest concentration of essential amino acids was found in C-44 followed by KK-2, KK-1 and Fakhr E Tal while the lowest concentration was recorded in Cm-98, Paidar-91 and Sheen Ghar-2000 respectively. Cultivars TAL-2006, Chattan and Karak-3 showed maximum concentration of both essential and endogenous amino acids. In conclusion; for broadening the genetic pools in breeding programs or to search for exotic characters, for instance new disease resistance alleles, accession with low similarity coefficients (Lawaghar and Battal-98) may be utilized. Furthermore the information acquired from this study could be used to device a proficient breeding approach intended at improving nutritional as well as broadening the genetic base of this essential food crop of Pakistan.

Resumo As distâncias genéticas entre as diferentes variedades de grão-de-bico e a avaliação de seus perfis de aminoácidos livres foram determinadas com base na eletroforese em gel de poliacrilamida com dodecil sulfato de sódio (SDS-PAGE). As proteínas solúveis totais foram resolvidas em SDS-PAGE a 10%. Foi observada baixa variabilidade nas variedades testadas. O dendrograma fundamentado em dados eletroforéticos agrupou os genótipos em dois grupos. Os resultados mostraram que o teor médio de proteínas de todas as variedades foi de 26,01%, na faixa de 22,8% para Thal-2006 a 34,06% para Sheenghar-2000 do peso de sementes secas. Com base no conteúdo total de proteínas, Bittal-98, Dasht, Sheen Ghar-2000, Karak-3, CM-98, Paidar-91, Fakhr-e-Thal, C-44, Balaksar e KK-1 apresentaram concentrações semelhantes para o conteúdo de proteínas entre si, mas tiveram variação quanto ao restante das variedades. Diferentes proteínas foram separadas com base nas alterações de seus pesos moleculares por meio de eletroforese em gel de poliacrilamida com dodecil sulfato de sódio (SDS-PAGE). Dasht, CM-98 e Sheen Ghar mostraram 100% de similaridade. Balaksar, Fakhr-e-Thal, KK-2, Chattan e KC-98, KK-1 e Lawaghar foram 100% semelhantes entre si, mas apresentaram variação em relação ao restante dos acessos. O dendrograma geral mostrou alto e baixo nível de variação entre os acessos. A concentração de aminoácidos livres variou entre as 16 variedades de grão-de-bico. Foi encontrada uma diferença significativa entre os aminoácidos essenciais e não essenciais nas cultivares de grão-de-bico. A concentração total de aminoácidos essenciais foi registrada em 40,81 g / 100 g de proteína, enquanto a não essencial foi registrada em 59,18343 g / 100 g de proteína nas cultivares. A maior concentração de aminoácidos essenciais foi encontrada em C-44, seguida de KK-2, KK-1 e Fakhr-e-Thal, enquanto a menor concentração foi registrada em CM-98, Paidar-91 e Sheen Ghar-2000. As cultivares TAL-2006, Chattan e Karak-3 apresentaram concentração máxima de aminoácidos essenciais e endógenos. Em conclusão, para ampliar os pools genéticos em programas de melhoramento ou procurar caracteres exóticos, por exemplo, novos alelos de resistência a doenças, pode ser utilizada a adesão com baixos coeficientes de similaridade (Lawaghar e Battal-98). Além disso, as informações adquiridas neste estudo poderiam ser usadas para criar uma abordagem de criação eficiente, com o objetivo de melhorar a nutrição e ampliar a base genética dessa cultura alimentar essencial do Paquistão.

Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea

The main objective of the present study was to isolate and characterize actinomycetes for their plant growth-promotion in chickpea. A total of 89 actinomycetes were screened for their antagonism against fungal pathogens of chickpea by dual culture and metabolite production assays. Four most promising actinomycetes were evaluated for their physiological and plant growth-promotion properties under in vitro and in vivo conditions. All the isolates exhibited good growth at temperatures from 20 °C to 40 °C, pH range of 711 and NaCl concentrations up to 8%. These were also found highly tolerant to Bavistin, slightly tolerant to Thiram and Captan (except VAI-7 and VAI-40) but susceptible to Benlate and Ridomil at field application levels and were found to produce siderophore, cellulase, lipase, protease, chitinase (except VAI-40), hydrocyanic acid (except VAI-7 and VAI-40), indole acetic acid and -1,3-glucanase. When the four actinomycetes were evaluated for their plant growth-promotion properties under field conditions on chickpea, all exhibited increase in nodule number, shoot weight and yield. The actinomycetes treated plots enhanced total N, available P and organic C over the un-inoculated control. The scanning electron microscope studies exhibited extensive colonization by actinomycetes on the root surface of chickpea. The expression profiles for indole acetic acid, siderophore and -1,3-glucanase genes exhibited up-regulation for all three traits and in all four isolates. The actinomycetes were identified as Streptomyces but different species in the 16S rDNA analysis. It was concluded that the selected actinomycetes have good plant growth-promotion and biocontrol potentials on chickpea. (AU)

Starches from non - conventional sources to improve the technological characteristics of pound cake

This study evaluated and compared the effect of the utilization of five different non-conventional starches (chickpea, common bean, Peruvian carrot, sweet potato and white bean) and four different commercial starches (cassava, corn, potato and rice) in pound cake. Common bean starch, followed by Peruvian carrot starch were the non-conventional starch sources that showed tendency to improve the technological quality of pound cake, mainly in relation to corn starch, the most common commercial source. With these sources, the batters presented lower specific gravity and the cakes presented higher specific volume, lighter color, lower crumb moisture reduction during the storage period, and better texture attributes during all the cake shelf-life. Moreover, common bean starch provided higher scores in the cake sensory evaluation; especially for grain and texture attributes (moisture, tenderness and softness). Chickpea and white bean starches were more similar to corn starch in pound cake application.

Este estudo avaliou e comparou o efeito da utilização de cinco amidos de fontes não convencionais (grão de bico, feijão carioca, mandioquinha, batata doce e feijão branco) e de quatro diferentes amidos de fontes comerciais (mandioca, milho, batata e arroz) em bolo inglês. Amido de feijão carioca, seguido pelo amido de mandioquinha, foram os amidos de fontes não convencionais que mostraram tendência a melhorar a qualidade tecnológica do bolo inglês, principalmente em comparação com o amido de milho, o qual é a fonte comercial mais utilizada. Com o amido dessas fontes, os batidos apresentaram menores valores de gravidade específica e os bolos maiores valores de volume específico, cor mais clara, menor redução da umidade durante o período de estocagem e melhores valores nos atributos de textura durante a vida de prateleira. Além disso, amido de feijão carioca promoveu maiores escores na avalição sensorial; especialmente para os atributos do miolo e textura (umidade, suavidade e maciez). Amidos de grão de bico e feijão branco foram muito similares ao amido de milho em aplicação em bolo inglês.

Starches from non - conventional sources to improve the technological characteristics of pound cake

This study evaluated and compared the effect of the utilization of five different non-conventional starches (chickpea, common bean, Peruvian carrot, sweet potato and white bean) and four different commercial starches (cassava, corn, potato and rice) in pound cake. Common bean starch, followed by Peruvian carrot starch were the non-conventional starch sources that showed tendency to improve the technological quality of pound cake, mainly in relation to corn starch, the most common commercial source. With these sources, the batters presented lower specific gravity and the cakes presented higher specific volume, lighter color, lower crumb moisture reduction during the storage period, and better texture attributes during all the cake shelf-life. Moreover, common bean starch provided higher scores in the cake sensory evaluation; especially for grain and texture attributes (moisture, tenderness and softness). Chickpea and white bean starches were more similar to corn starch in pound cake application.

Este estudo avaliou e comparou o efeito da utilização de cinco amidos de fontes não convencionais (grão de bico, feijão carioca, mandioquinha, batata doce e feijão branco) e de quatro diferentes amidos de fontes comerciais (mandioca, milho, batata e arroz) em bolo inglês. Amido de feijão carioca, seguido pelo amido de mandioquinha, foram os amidos de fontes não convencionais que mostraram tendência a melhorar a qualidade tecnológica do bolo inglês, principalmente em comparação com o amido de milho, o qual é a fonte comercial mais utilizada. Com o amido dessas fontes, os batidos apresentaram menores valores de gravidade específica e os bolos maiores valores de volume específico, cor mais clara, menor redução da umidade durante o período de estocagem e melhores valores nos atributos de textura durante a vida de prateleira. Além disso, amido de feijão carioca promoveu maiores escores na avalição sensorial; especialmente para os atributos do miolo e textura (umidade, suavidade e maciez). Amidos de grão de bico e feijão branco foram muito similares ao amido de milho em aplicação em bolo inglês.

Phosphate solubilization potential and phosphatase activity of rhizospheric Trichoderma spp.

Trichoderma sp., a well known biological control agent against several phytopathogens, was tested for its phosphate (P) solubilizing potential. Fourteen strains of Trichoderma sp. were isolated from the forest tree rhizospheres of pinus, deodar, bamboo, guava and oak on Trichoderma selective medium. The isolates were tested for their in-vitro P-solubilizing potential using National Botanical Research Institute Phosphate (NBRIP) broth containing tricalcium phosphate (TCP) as the sole P source, and compared with a standard culture of T. harzianum. All the cultures were found to solubilize TCP but with varying potential. The isolate DRT-1 showed maximum amount of soluble phosphate (404.07 µg.ml-1), followed by the standard culture of T. harzianum (386.42 µg.ml-1) after 96 h of incubation at 30+1(0)C. Extra-cellular acid and alkaline phosphatases of the fungus were induced only in the presence of insoluble phosphorus source (TCP). High extra-cellular alkaline phosphatase activity was recorded for the isolate DRT-1 (14.50 U.ml-1) followed by the standard culture (13.41 U.ml-1) at 72h. The cultures showed much lesser acid phosphatase activities. Under glasshouse conditions, Trichoderma sp. inoculation increased chickpea (Cicer arietinum) growth parameters including shoot length, root length, fresh and dry weight of shoot as well as roots, in P-deficient soil containing only bound phosphate (TCP). Shoot weight was increased by 23% and 33% by inoculation with the isolate DRT-1 in the soil amended with 100 and 200 mg TCP kg-1 soil, respectively, after 60 d of sowing. The study explores high P-solubilizing potential of Trichoderma sp., which can be exploited for the solubilization of fixed phosphates present in the soil, thereby enhancing soil fertility and plant growth.

Phosphate solubilization potential and phosphatase activity of rhizospheric Trichoderma spp.

Trichoderma sp., a well known biological control agent against several phytopathogens, was tested for its phosphate (P) solubilizing potential. Fourteen strains of Trichoderma sp. were isolated from the forest tree rhizospheres of pinus, deodar, bamboo, guava and oak on Trichoderma selective medium. The isolates were tested for their in-vitro P-solubilizing potential using National Botanical Research Institute Phosphate (NBRIP) broth containing tricalcium phosphate (TCP) as the sole P source, and compared with a standard culture of T. harzianum. All the cultures were found to solubilize TCP but with varying potential. The isolate DRT-1 showed maximum amount of soluble phosphate (404.07 µg.ml-1), followed by the standard culture of T. harzianum (386.42 µg.ml-1) after 96 h of incubation at 30+1(0)C. Extra-cellular acid and alkaline phosphatases of the fungus were induced only in the presence of insoluble phosphorus source (TCP). High extra-cellular alkaline phosphatase activity was recorded for the isolate DRT-1 (14.50 U.ml-1) followed by the standard culture (13.41 U.ml-1) at 72h. The cultures showed much lesser acid phosphatase activities. Under glasshouse conditions, Trichoderma sp. inoculation increased chickpea (Cicer arietinum) growth parameters including shoot length, root length, fresh and dry weight of shoot as well as roots, in P-deficient soil containing only bound phosphate (TCP). Shoot weight was increased by 23% and 33% by inoculation with the isolate DRT-1 in the soil amended with 100 and 200 mg TCP kg-1 soil, respectively, after 60 d of sowing. The study explores high P-solubilizing potential of Trichoderma sp., which can be exploited for the solubilization of fixed phosphates present in the soil, thereby enhancing soil fertility and plant growth.

GRÃO-DE-BICO

Chickpea is the third most important leguminous plant used as human food due its digestibility and biological value of the protein. Is a winter crop in India, Ethiopia and Latin America whereas it is a spring crop in the Mediterranean countries. Most of the cultivated area is located, between 15 and 40° North latitude and about 10% is irrigated. Total world production is 5,6 million tons grown in an area of 9,6 million hectares. The low productivity observed are attributed to a lack of appropriate cultivars, low tecnology levels and diseases mainly in India and Pakistan that are responsible for about 85% of total production. In Brazil this crop is incipient and its consumption very low. However since it is a winter crop and of high quality protein it has a great potencial as one more food alternative. Therefore publication of data about origen, taxonomy, importance, use and above all, information about the management of this crop will be useful to people interested in it. Also are shown some research data obtained in Brazil.

O grão-de-bico (Cicer arietinum), considerado entre as leguminosas alimentícias como o terceiro mais importante cultivo no mundo, possui em sua proteína um dos maiores valores biológicos devido a maior digestibilidade. É um cultivo de inverno no sub-continente Indiano, Etiópia e América Latina, enquanto na região do Mediterrâneo é cultivado na primavera Em geral, seu cultivo ocorre entre as latitudes de 15° e 40° N onde, aproximadamente, 10% da área é irrigada. A produção mundial é de 5,6 milhões de toneladas e a área semeada é de 9,6 milhões de hectares. O baixo rendimento se deve, principalmente, a falta de cultivares apropriadas para cada região, o baixo nível tecnológico empregado e o ataque de doenças que, periodicamente, destroem a lavoura Essas observações são válidas para a Índia e Paquistão que juntos contribuem com cerca de 85% da produção mundial. No Brasil, o cultivo do grão-de-bico é incipiente e seu consumo muito pequeno. No entanto, como se trata de uma leguminosa de inverno com proteína de alta qualidade, possui grande potencial como cultivo alternativo. Em função disso, a divulgação de dados sobre origem, taxonomia, importância, utilização e, principalmente, subsídios para o cultivo da espécie serão muito úteis aos interessados na produção desta leguminosa Neste trabalho são mostrados, também, alguns dados de pesquisa obtidos recentemente no Brasil.

Growth analysis of chickpea (Cicer arietinum L.)

An experiment to study the growing pattern of a chickpea variety, IAC-Marrocos, was carried out at the Monte Alegre Experimental Station, SP, during 1987 and 1988. The dry matter production of all parts of the plant, as well the leaf area index, were weekly evaluated. Exponential quadratic models of regression were adjusted to total dry matter, leaf dry matter and leaf area index, and a linear model to dry matter of grain. Based on the growth analysis it was concluded that the chickpea is a rustic eatable plant that can be recommended as an alternative winter crop for similar climatic conditions as those of the experiment.

Com a finalidade de avaliar o padrão de crescimento do grão-de-bico, variedade IAC-Marrocos, realizou-se um experimento na Estação Experimental de Monte Alegre do Sul, SP, nos anos agrícolas de 1987 e 1988. Semanalmente eram feitas coletas de plantas e após a separação das partes, eram obtidos os pesos secos, bem como o índice de área foliar. Esses dados foram analisados com modelos de regressão linear e exponencial ajustados para as matérias secas de grão, de folha e total e para os índices de área foliar e de crescimento. Baseando-se na análise de crescimento conclui-se que o grão-de-bico é uma planta que pode ser recomendada como alternativa para a cultura de inverno em condições climáticas semelhantes às do presente estudo.

Grão-de-bico

Chickpea is the third most important leguminous plant used as human food due its digestibility and biological value of the protein. Is a winter crop in India, Ethiopia and Latin America whereas it is a spring crop in the Mediterranean countries. Most of the cultivated area is located, between 15 and 40° North latitude and about 10% is irrigated. Total world production is 5,6 million tons grown in an area of 9,6 million hectares. The low productivity observed are attributed to a lack of appropriate cultivars, low tecnology levels and diseases mainly in India and Pakistan that are responsible for about 85% of total production. In Brazil this crop is incipient and its consumption very low. However since it is a winter crop and of high quality protein it has a great potencial as one more food alternative. Therefore publication of data about origen, taxonomy, importance, use and above all, information about the management of this crop will be useful to people interested in it. Also are shown some research data obtained in Brazil.

O grão-de-bico (Cicer arietinum), considerado entre as leguminosas alimentícias como o terceiro mais importante cultivo no mundo, possui em sua proteína um dos maiores valores biológicos devido a maior digestibilidade. É um cultivo de inverno no sub-continente Indiano, Etiópia e América Latina, enquanto na região do Mediterrâneo é cultivado na primavera Em geral, seu cultivo ocorre entre as latitudes de 15° e 40° N onde, aproximadamente, 10% da área é irrigada. A produção mundial é de 5,6 milhões de toneladas e a área semeada é de 9,6 milhões de hectares. O baixo rendimento se deve, principalmente, a falta de cultivares apropriadas para cada região, o baixo nível tecnológico empregado e o ataque de doenças que, periodicamente, destroem a lavoura Essas observações são válidas para a Índia e Paquistão que juntos contribuem com cerca de 85% da produção mundial. No Brasil, o cultivo do grão-de-bico é incipiente e seu consumo muito pequeno. No entanto, como se trata de uma leguminosa de inverno com proteína de alta qualidade, possui grande potencial como cultivo alternativo. Em função disso, a divulgação de dados sobre origem, taxonomia, importância, utilização e, principalmente, subsídios para o cultivo da espécie serão muito úteis aos interessados na produção desta leguminosa Neste trabalho são mostrados, também, alguns dados de pesquisa obtidos recentemente no Brasil.

Growth analysis of chickpea (Cicer arietinum L.)

An experiment to study the growing pattern of a chickpea variety, IAC-Marrocos, was carried out at the Monte Alegre Experimental Station, SP, during 1987 and 1988. The dry matter production of all parts of the plant, as well the leaf area index, were weekly evaluated. Exponential quadratic models of regression were adjusted to total dry matter, leaf dry matter and leaf area index, and a linear model to dry matter of grain. Based on the growth analysis it was concluded that the chickpea is a rustic eatable plant that can be recommended as an alternative winter crop for similar climatic conditions as those of the experiment.

Com a finalidade de avaliar o padrão de crescimento do grão-de-bico, variedade IAC-Marrocos, realizou-se um experimento na Estação Experimental de Monte Alegre do Sul, SP, nos anos agrícolas de 1987 e 1988. Semanalmente eram feitas coletas de plantas e após a separação das partes, eram obtidos os pesos secos, bem como o índice de área foliar. Esses dados foram analisados com modelos de regressão linear e exponencial ajustados para as matérias secas de grão, de folha e total e para os índices de área foliar e de crescimento. Baseando-se na análise de crescimento conclui-se que o grão-de-bico é uma planta que pode ser recomendada como alternativa para a cultura de inverno em condições climáticas semelhantes às do presente estudo.