Transcriptome Analysis of Chicken Embryo Fibroblast Cell Infected with Mareks Disease Virus of GX0101 LTR

Mareks disease (MD), a lymphoproliferative disorder of chickens caused by the MD virus (MDV), is economically significant. The resistance/susceptibility to MD is controlled by host genetics. The host response to different virus strains varies. The pathogenicity of REV-LTR deleted GX0101LTR MDV has been previously reported. However, the precise molecular mechanism of the response of chickens to GX0101LTR remains unclear. The current study aimed at identifying the genes and pathways involved in the response to GX0101LTR virus infection in specific pathogen-free chicken embryo fibroblast cells using global transcriptome analysis. A total of 1,633 genes associated with GX0101LTR infection were identified. Functional analysis showed that the cytokine-cytokine receptor interaction plays an important role in the response to GX0101LTR infection.(AU)

Transcriptome Analysis of Chicken Embryo Fibroblast Cell Infected with Mareks Disease Virus of GX0101 LTR

Mareks disease (MD), a lymphoproliferative disorder of chickens caused by the MD virus (MDV), is economically significant. The resistance/susceptibility to MD is controlled by host genetics. The host response to different virus strains varies. The pathogenicity of REV-LTR deleted GX0101LTR MDV has been previously reported. However, the precise molecular mechanism of the response of chickens to GX0101LTR remains unclear. The current study aimed at identifying the genes and pathways involved in the response to GX0101LTR virus infection in specific pathogen-free chicken embryo fibroblast cells using global transcriptome analysis. A total of 1,633 genes associated with GX0101LTR infection were identified. Functional analysis showed that the cytokine-cytokine receptor interaction plays an important role in the response to GX0101LTR infection.

Detection of Maedi-Visna virus from sheep bronchoalveolar lavage by Nested PCR evaluation of different primers pairs / Detecção do vírus Maedi-Visna em amostras de lavado bronco-alveolar de ovinos através da técnica de Nested PCR utilizando diferentes pares de primers

Background: Small ruminant lentiviruses (SRLV) are characterized by a high degree of genetic variability related to your replication process, resulting in several strains in different geographic regions. The Polymerase Chain Reaction (PCR) is very successful in the detection of proviral DNA of SRLV, however, due to the high variability of the lentivirus genome, the efficiency and sensibility of PCR depends mainly on the specificity of the primers designed and the choice of the amplified target viral region. The aim of this study was to compare detection of Maedi Visna Virus (MVV) from bronco alveolar lavage samples of sheep by Nested PCR using primers for the gag and LTR genes. Materials, Methods & Results: Samples of sheep bronchoalveolar lavage (n = 58) from slaughterhouse in the Metropolitan Region of Fortaleza were previously tested by nested PCR using primers for region gag. Thereafter, these samples were tested by nested PCR with primers designed for the LTR region. Both tests were conducted using thermocycler (Biocycler®) under the following conditions: initial denaturation at 94C for 5 min, followed by 35 cycles of denaturation at 94C for 1 min, annealing of primers at 56C for 1 min and extension of DNA at 72C for 45 s with a final extension at 72 for 7 min. The first and second round were performed under the same conditions. Every amplification was performed [...](AU)

Detection of Maedi-Visna virus from sheep bronchoalveolar lavage by Nested PCR evaluation of different primers pairs / Detecção do vírus Maedi-Visna em amostras de lavado bronco-alveolar de ovinos através da técnica de Nested PCR utilizando diferentes pares de primers

Background: Small ruminant lentiviruses (SRLV) are characterized by a high degree of genetic variability related to your replication process, resulting in several strains in different geographic regions. The Polymerase Chain Reaction (PCR) is very successful in the detection of proviral DNA of SRLV, however, due to the high variability of the lentivirus genome, the efficiency and sensibility of PCR depends mainly on the specificity of the primers designed and the choice of the amplified target viral region. The aim of this study was to compare detection of Maedi Visna Virus (MVV) from bronco alveolar lavage samples of sheep by Nested PCR using primers for the gag and LTR genes. Materials, Methods & Results: Samples of sheep bronchoalveolar lavage (n = 58) from slaughterhouse in the Metropolitan Region of Fortaleza were previously tested by nested PCR using primers for region gag. Thereafter, these samples were tested by nested PCR with primers designed for the LTR region. Both tests were conducted using thermocycler (Biocycler®) under the following conditions: initial denaturation at 94C for 5 min, followed by 35 cycles of denaturation at 94C for 1 min, annealing of primers at 56C for 1 min and extension of DNA at 72C for 45 s with a final extension at 72 for 7 min. The first and second round were performed under the same conditions. Every amplification was performed [...]

SOROPREVALÊNCIA E CARACTERIZAÇÃO GENÉTICA DE ESTIRPES DE CAMPO DO VÍRUS DA ANEMIA INFECCIOSA EQUINA EM EQUÍDEOS ERRANTES DO ESTADO DO RIO GRANDE DO NORTE

A anemia infecciosa equina (AIE) é a doença infecciosa de etiologia viral mais importante entre os equinos. É uma doença endêmica em populações de equídeos por todo o mundo. Considera-se que todos os equídeos são susceptíveis embora os trabalhos publicados se concentrem em equinos, sendo escassas as informações sobre AIE em asininos. No Rio Grande do Norte (RN) milhares de asininos vivem livres, constituindo um problema para o controle de enfermidades como a AIE, pois podem ser prováveis reservatórios e fontes de transmissão delas. Neste trabalho, amostras de 409 animais (asininos e equinos) foram submetidas aos testes de IDGA, ELISA pgp45 e nested-PCR, utilizando iniciadores para o gene gag e LTR. Quatro amostras (0,98%) foram positivas em pelo menos um teste sorológico, e dessas, três amostras (0,73%) foram positivas na nested-PCR. Não obtivemos material suficiente para sequenciamento das amostras de LTR dos asininos sendo sequenciado apenas o amplificado de LTR referente à amostra do equino positivo. O alinhamento (BLAST) permitiu a identificação da sequência como vírus da AIE (EIAV) com 95% de similaridade de nucleotídeos com estirpes europeias. Os três produtos obtidos da nested-PCR para o gene gag foram sequenciados e submetidos à análise filogenética. O resultado da análise sugeriu que a sequência obtida do cavalo confirmaram a identificação como EIAV, com a mesma origem de isolados da América do Norte, e que as sequencias de asininos não possuem identidade com nenhuma outra deo EIAV até o momento publicada. Desta forma, estudos adicionais são necessários para confirmar se, com estes resultados, foram identificados um lentivirus ainda não descrito que infecta Equus asinus (e qual papel ele teria para outros equídeos) ou se trata de retrovírus endógenos que expressa proteínas usadas como marcadores de diagnóstico da AIE. Embora os dados apresentados sejam incipientes, revelam um cenário interessante para o estudo das lentiviroses em asininos.

Equine infectious anemia (EIA) is the most important viral disease among horses. It is an endemic disease in populations of Equidae throughout the world. All equids are considered to be susceptible although most of the published work are focused in horses, with a lack of information on EIA in donkeys. In the State of Rio Grande do Norte, Brazil thousands of donkeys live free, establishing a problem for the control of diseases like the EIA, since they may play a role as reservoirs. In this work, blood samples taken from 409 animals (asinine and equine) were submitted to IDGA, ELISA pgp45 and nested-PCR tests, using gag and LTR gene as molecular markers. Four samples (0.98%) were positive in at least one serological test, and of these, three (0.73%) were positive to nested-PCR. We did not get enough material for sequencing of LTR samples from asinine, then only the amplified referring to the positive equine sample was sequenced. The alignment (BLAST) allowed the identification of the sequence as EIAV with 95% of similarity with european strains. Three product from nested-PCR products for the gag gene were sequenced and submitted to phylogenetic analysis. The result of this analysis suggested that the samples obtained from the horse had the same origin as strains from North America, and that the sequences from donkey samples had no homology with any other already published available EIAV sequence. In the light of the results from this work, additional studies are required. So we could confirm that we found a new strain of EIAV or endogenous retrovirus that are capable of expressing genes that are homologous to EIAV or even a new species of lentivirus Although the presented data are incomplete, they reveal an interesting scenario for the study of EIAV infection in equids ther than the horses.