Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates

A shift towards declining azoxystrobin sensitivity has been previously documented in a collection of Brazilian Monilinia fructicola isolates, but information on the stability of this trait after in vitro sub-culturing is not available. In this study, one highly-sensitive isolate and two isolates with reduced sensitivity to azoxystrobin, not exhibiting point mutations at codons 129, 137 and 143 in the target gene of cytochrome b were used. Two independent experiments consisted of 10 weekly transfers of each strain in potato dextrose agar (PDA). Conidial production and germination were quantified in the initial culture and, again, after the third, sixth and tenth transfer. Measures of mycelial growth were obtained in colonies growing on PDA amended with azoxystrobin at 1 µg mL-1 and salicylhydroxamic acid at 100 µg mL-1. Data were normalized to mycelial growth rate after each of the 10 transfers. The colony diameter in the less sensitive isolates was stable across the transfers in the PDA amended with the fungicide. The conidial production and mycelial growth rate did not decrease in non-amended media either. Our results suggest that the trait of reduced sensitivity to azoxystrobin is stable under no selection pressure for azoxystrobin.

Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates

A shift towards declining azoxystrobin sensitivity has been previously documented in a collection of Brazilian Monilinia fructicola isolates, but information on the stability of this trait after in vitro sub-culturing is not available. In this study, one highly-sensitive isolate and two isolates with reduced sensitivity to azoxystrobin, not exhibiting point mutations at codons 129, 137 and 143 in the target gene of cytochrome b were used. Two independent experiments consisted of 10 weekly transfers of each strain in potato dextrose agar (PDA). Conidial production and germination were quantified in the initial culture and, again, after the third, sixth and tenth transfer. Measures of mycelial growth were obtained in colonies growing on PDA amended with azoxystrobin at 1 µg mL-1 and salicylhydroxamic acid at 100 µg mL-1. Data were normalized to mycelial growth rate after each of the 10 transfers. The colony diameter in the less sensitive isolates was stable across the transfers in the PDA amended with the fungicide. The conidial production and mycelial growth rate did not decrease in non-amended media either. Our results suggest that the trait of reduced sensitivity to azoxystrobin is stable under no selection pressure for azoxystrobin.(AU)

COENZIMA Q10 EM DIETA PARA CODORNA JAPONESA (Coturnix japonica domestica) EM FASE DE POSTURA

Esse estudo teve como objetivo avaliar o desempenho produtivo de codornas de postura alimentadas com diferentes níveis de Coenzima Q10 (CoQ10). O ensaio foi instalado em delineamento experimental inteiramente casualizado, em esquema de análise fatorial 3x3 analisado como medidas repetidas no tempo, com 5 repetições por tratamento (12 aves por box). O fator (A) foi constituído por 3 tratamentos (T1. sem adição de CoQ10 na ração; T2. ração com adição de 100 mg CoQ10.kg-1 MS; T3. ração com adição de 200 mg CoQ10.kg-1 MS). O fator (B), por três períodos experimentais (C1. de 1 a 28 dias de suplementação; C2. período de 29 a 56 dias de suplementação e, C3. período de 57 a 84 dias de suplementação). Foram avaliados o consumo de ração, taxa de postura, peso médio dos ovos e conversão alimentar. Não houve interação entre as doses de CoQ10 e os ciclos produtivos e, apenas o peso do ovo não apresentou significância para o fator dose de CoQ10. O consumo de ração aumentou linearmente, a taxa de postura e a conversão alimentar apresentaram resposta quadrática com ponto de máximo a 87,43 mg CoQ10 kg-1 e 100,17 mg CoQ10 kg-1, respectivamente. O fator ciclo produtivo apresentou resposta quadrática com ponto máximo de consumo de ração aos 53 dias de experimentação, a taxa de postura decresceu com o decorrer do experimento e o inverso ocorreu com o peso do ovo e a conversão alimentar. A inclusão de 100 mg CoQ10 kg-1 MS na ração para codornas japonesas, em fase inicial, aumenta a taxa de postura, o que resulta em melhor conversão alimentar o que pode indicar uma possível ação sobre processos fisiológicos reprodutivos. A inclusão de 200 mg CoQ10 kg-1 MS de ração não traz benefícios produtivos e, em função da redução no consumo de ração, pode representar o limite superior de inclusão da CoQ10 na dieta da ave.

This study aimed to evaluate the productive performance of laying quails fed different levels of Coenzyme Q10 (CoQ10). The trial was installed in a completely randomized design, in a 3x3 factorial analysis scheme analyzed as repeated measures over time, with 5 replicates per treatment (12 birds per box). Factor (A) consisted of 3 treatments (T1. Without addition of CoQ10 in the diet; T2. Diet with addition of 100 mg CoQ10.kg-1 MS; T3. Diet with addition of 200 mg CoQ10.kg-1 MS). Factor (B), for three experimental periods (C1. From 1 to 28 days of supplementation; C2. From 29 to 56 days of supplementation; and C3. From 57 to 86 days of supplementation). Feed intake, laying rate, average egg weight and feed conversion were evaluated. There was no interaction between the doses of CoQ10 and the production cycles, and only the weight of the egg was not significant for the dose factor of CoQ10. Feed intake increased linearly, the laying rate and feed conversion showed a quadratic response with a maximum point at 87.43 mg CoQ10 kg-1 and 100.17 mg CoQ10 kg- 1, respectively. The productive cycle factor showed a quadratic response with the maximum point of feed consumption after 53 days of experimentation, the laying rate decreased with the course of the experiment and the reverse occurred with egg weight and feed conversion. The inclusion of 100 mg CoQ10 kg-1 MS in the diet for Japanese quails, in the initial phase, increases the laying rate, which results in a better feed conversion which may indicate a possible action on reproductive physiological processes. The inclusion of 200 mg CoQ10 kg-1 DM of feed does not bring productive benefits and, due to the reduction in feed consumption, may represent the upper limit of inclusion of CoQ10 in the bird's diet.

Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66

Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.

Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing

In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh) gene that involved in the phosphate solublization using cofactor pyrroloquinolone quinone (PQQ). Seven rhizoshperic and 3 root nodulating stains are positive for gdh gene. Furthermore, this study confirms a novel association between microbes and their hosts like field grown crops, leguminous and non-leguminous plants. It was concluded that a diverse group of bacterial population exist in the rhizosphere and root nodules that might be useful in evaluating the mechanisms behind plant microbial interactions and strains QAU-63 and QAU-68 have sequence similarity of 97 and 95% which might be declared as novel after further taxonomic characterization.

Use of P450 cytochrome inhibitors in studies of enokipodin biosynthesis

Enokipodins A, B, C, and D are antimicrobial sesquiterpenes isolated from the mycelial culture medium of Flammulina velutipes, an edible mushroom. The presence of a quaternary carbon stereocenter on the cyclopentane ring makes enokipodins A-D attractive synthetic targets. In this study, nine different cytochrome P450 inhibitors were used to trap the biosynthetic intermediates of highly oxygenated cuparene-type sesquiterpenes of F. velutipes. Of these, 1-aminobenzotriazole produced three less-highly oxygenated biosynthetic intermediates of enokipodins A-D; these were identified as (S)-(-)-cuparene-1,4-quinone and epimers at C-3 of 6-hydroxy-6-methyl-3-(1,2,2-trimethylcyclopentyl)-2-cyclohexen-1-one. One of the epimers was found to be a new compound.

Sorgoleone: benzoquinona lipídica de sorgo com efeitos alelopáticos na agricultura como herbicida

The sorghum crop has grown rapidly in recent years, for being a plant with xerophilous characteristics; its production has especially increased in Northeastern Brazil due to its ability to withstand dry environments. Weeds are a major problem for grain crops, as these can significantly reduce yield, particularly when they emerge in the early stages of crops. To obtain crops resistant to weeds, studies have been conducted to demonstrate that some plants have a natural defense consisting in an organism's ability to produce metabolites that act to either inhibit or stimulate growth and development of other organisms grown in the same environment, which is called allelopathy. Sorghum is one of the plants that possess proven allelopathy, producing a set of lipid complex substances and proteins generically called sorgoleone, having as its main compound 2-hydroxy-5-methoxy-3-[(Z,Z)-8',11',14'-pentadecatriene]-p-benzoquinone, which is naturally released into the soil from their roots and trichomes, inhibiting the growth of weeds upon contact with them. Because of these inherent characteristics of the sorghum crop, the present work aims to discuss the possible benefits of using this cereal, due to its allelopathic effects, as well as to inform about the known mechanisms of production and action of the main constituent compounds of sorgoleone and other compounds produced by the plant's roots.

A cultura do sorgo cresceu rapidamente nestes últimos anos, por ser uma planta com características xerófilas, apresentando um aumento de sua produção principalmente na região nordeste devido a sua capacidade de suportar ambientes de cultivo mais secos. As ervas daninhas são um grande problema para os cultivares, pois estas podem reduzir significativamente a produção de grãos, particularmente quando surgem nas fases iniciais das culturas. Visando a obtenção de culturas resistentes às ervas daninhas, estudos têm sido realizados demonstrando que algumas plantas possuem uma defesa natural que consiste na capacidade de um organismo produzir metabólitos que atuam inibindo ou o crescimento ou o desenvolvimento de outros organismos que estão próximos; a esta capacidade dá-se o nome de alelopatia. O sorgo é uma das plantas que possuem sua alelopatia comprovada, produzindo um complexo de substâncias lipídicas e proteínas denominados genericamente de sorgoleone, tendo como seu principal composto o 2-hidroxi-5-metoxi-3-[(Z,Z)-8',11',14'-pentadecatrieno]-p-benzoquinona, que é naturalmente liberado para o solo a partir dos tricomas das suas raízes e, no momento em que entram em contato com as ervas daninhas, inibem seu crescimento. Devido a tais características inerentes à cultura do sorgo, este trabalho tem como objetivo discorrer sobre os possíveis benefícios do uso desse cereal devido a sua comprovada alelopatia, bem como informar os conhecidos mecanismos de produção e atuação dos principais compostos constituintes do sorgoleone produzidos pelas suas raízes.

Sorgoleone: benzoquinona lipídica de sorgo com efeitos alelopáticos na agricultura como herbicida / Sorgoleone: lipidic benzoquinone of sorghum with allelopathic effects in agriculture as a herbicide

A cultura do sorgo cresceu rapidamente nestes últimos anos, por ser uma planta com características xerófilas, apresentando um aumento de sua produção principalmente na região nordeste devido a sua capacidade de suportar ambientes de cultivo mais secos. As ervas daninhas são um grande problema para os cultivares, pois estas podem reduzir significativamente a produção de grãos, particularmente quando surgem nas fases iniciais das culturas. Visando a obtenção de culturas resistentes às ervas daninhas, estudos têm sido realizados demonstrando que algumas plantas possuem uma defesa natural que consiste na capacidade de um organismo produzir metabólitos que atuam inibindo ou o crescimento ou o desenvolvimento de outros organismos que estão próximos; a esta capacidade dá-se o nome de alelopatia. O sorgo é uma das plantas que possuem sua alelopatia comprovada, produzindo um complexo de substâncias lipídicas e proteínas denominados genericamente de sorgoleone, tendo como seu principal composto o 2-hidroxi-5--metoxi-3-[(Z,Z)-8’,11’,14’-pentadecatrieno]-p-benzoquinona, que é naturalmente liberado para o solo a partir dos tricomas das suas raízes e, no momento em que entram em contato com as ervas daninhas, inibem seu crescimento. Devido a tais características inerentes à cultura do sorgo, este trabalho tem como objetivo discorrer sobre os possíveis benefícios do uso desse cereal devido a sua comprovada alelopatia, bem como informar os conhecidos mecanismos de produção e atuação dos principais compostos constituintes do sorgoleone produzidos pelas suas raízes.

SORGOLEONE: LIPIDIC BENZOQUINONE OF SORGHUM WITH ALLELOPATHIC EFFECTS IN AGRICULTURE AS A HERBICIDE. The sorghum crop has grown rapidly in recent years, for being a plant with xerophilous characteristics; its production has especially increased in Northeastern Brazil due to its ability to withstand dry environments. Weeds are a major problem for grain crops, as these can significantly reduce yield, particularly when they emerge in the early stages of crops. To obtain crops resistant to weeds, studies have been conducted to demonstrate that some plants have a natural defense consisting in an organism’s ability to produce metabolites that act to either inhibit or stimulate growth and development of other organisms grown in the same environment, which is called allelopathy. Sorghum is one of the plants that possess proven allelopathy, producing a set of lipid complex substances and proteins generically called sorgoleone, having as its main compound 2-hydroxy-5-methoxy-3-[(Z,Z)-8′,11′,14′-pentadecatriene]-p-benzoquinone, which is naturally released into the soil from their roots and trichomes, inhibiting the growth of weeds upon contact with them. Because of these inherent characteristics of the sorghum crop, the present work aims to discuss the possible benefits of using this cereal, due to its allelopathic effects, as well as to inform about the known mechanisms of production and action of the main constituent compounds of sorgoleone and other compounds produced by the plant’s roots.

Sorgoleone: benzoquinona lipídica de sorgo com efeitos alelopáticos na agricultura como herbicida

The sorghum crop has grown rapidly in recent years, for being a plant with xerophilous characteristics; its production has especially increased in Northeastern Brazil due to its ability to withstand dry environments. Weeds are a major problem for grain crops, as these can significantly reduce yield, particularly when they emerge in the early stages of crops. To obtain crops resistant to weeds, studies have been conducted to demonstrate that some plants have a natural defense consisting in an organism's ability to produce metabolites that act to either inhibit or stimulate growth and development of other organisms grown in the same environment, which is called allelopathy. Sorghum is one of the plants that possess proven allelopathy, producing a set of lipid complex substances and proteins generically called sorgoleone, having as its main compound 2-hydroxy-5-methoxy-3-[(Z,Z)-8',11',14'-pentadecatriene]-p-benzoquinone, which is naturally released into the soil from their roots and trichomes, inhibiting the growth of weeds upon contact with them. Because of these inherent characteristics of the sorghum crop, the present work aims to discuss the possible benefits of using this cereal, due to its allelopathic effects, as well as to inform about the known mechanisms of production and action of the main constituent compounds of sorgoleone and other compounds produced by the plant's roots.

A cultura do sorgo cresceu rapidamente nestes últimos anos, por ser uma planta com características xerófilas, apresentando um aumento de sua produção principalmente na região nordeste devido a sua capacidade de suportar ambientes de cultivo mais secos. As ervas daninhas são um grande problema para os cultivares, pois estas podem reduzir significativamente a produção de grãos, particularmente quando surgem nas fases iniciais das culturas. Visando a obtenção de culturas resistentes às ervas daninhas, estudos têm sido realizados demonstrando que algumas plantas possuem uma defesa natural que consiste na capacidade de um organismo produzir metabólitos que atuam inibindo ou o crescimento ou o desenvolvimento de outros organismos que estão próximos; a esta capacidade dá-se o nome de alelopatia. O sorgo é uma das plantas que possuem sua alelopatia comprovada, produzindo um complexo de substâncias lipídicas e proteínas denominados genericamente de sorgoleone, tendo como seu principal composto o 2-hidroxi-5-metoxi-3-[(Z,Z)-8',11',14'-pentadecatrieno]-p-benzoquinona, que é naturalmente liberado para o solo a partir dos tricomas das suas raízes e, no momento em que entram em contato com as ervas daninhas, inibem seu crescimento. Devido a tais características inerentes à cultura do sorgo, este trabalho tem como objetivo discorrer sobre os possíveis benefícios do uso desse cereal devido a sua comprovada alelopatia, bem como informar os conhecidos mecanismos de produção e atuação dos principais compostos constituintes do sorgoleone produzidos pelas suas raízes.

Sorgoleone: benzoquinona lipídica de sorgo com efeitos alelopáticos na agricultura como herbicida / Sorgoleone: lipidic benzoquinone of sorghum with allelopathic effects in agriculture as a herbicide

A cultura do sorgo cresceu rapidamente nestes últimos anos, por ser uma planta com características xerófilas, apresentando um aumento de sua produção principalmente na região nordeste devido a sua capacidade de suportar ambientes de cultivo mais secos. As ervas daninhas são um grande problema para os cultivares, pois estas podem reduzir significativamente a produção de grãos, particularmente quando surgem nas fases iniciais das culturas. Visando a obtenção de culturas resistentes às ervas daninhas, estudos têm sido realizados demonstrando que algumas plantas possuem uma defesa natural que consiste na capacidade de um organismo produzir metabólitos que atuam inibindo ou o crescimento ou o desenvolvimento de outros organismos que estão próximos; a esta capacidade dá-se o nome de alelopatia. O sorgo é uma das plantas que possuem sua alelopatia comprovada, produzindo um complexo de substâncias lipídicas e proteínas denominados genericamente de sorgoleone, tendo como seu principal composto o 2-hidroxi-5--metoxi-3-[(Z,Z)-8,11,14-pentadecatrieno]-p-benzoquinona, que é naturalmente liberado para o solo a partir dos tricomas das suas raízes e, no momento em que entram em contato com as ervas daninhas, inibem seu crescimento. Devido a tais características inerentes à cultura do sorgo, este trabalho tem como objetivo discorrer sobre os possíveis benefícios do uso desse cereal devido a sua comprovada alelopatia, bem como informar os conhecidos mecanismos de produção e atuação dos principais compostos constituintes do sorgoleone produzidos pelas suas raízes. (AU)

SORGOLEONE: LIPIDIC BENZOQUINONE OF SORGHUM WITH ALLELOPATHIC EFFECTS IN AGRICULTURE AS A HERBICIDE. The sorghum crop has grown rapidly in recent years, for being a plant with xerophilous characteristics; its production has especially increased in Northeastern Brazil due to its ability to withstand dry environments. Weeds are a major problem for grain crops, as these can significantly reduce yield, particularly when they emerge in the early stages of crops. To obtain crops resistant to weeds, studies have been conducted to demonstrate that some plants have a natural defense consisting in an organisms ability to produce metabolites that act to either inhibit or stimulate growth and development of other organisms grown in the same environment, which is called allelopathy. Sorghum is one of the plants that possess proven allelopathy, producing a set of lipid complex substances and proteins generically called sorgoleone, having as its main compound 2-hydroxy-5-methoxy-3-[(Z,Z)-8′,11′,14′-pentadecatriene]-p-benzoquinone, which is naturally released into the soil from their roots and trichomes, inhibiting the growth of weeds upon contact with them. Because of these inherent characteristics of the sorghum crop, the present work aims to discuss the possible benefits of using this cereal, due to its allelopathic effects, as well as to inform about the known mechanisms of production and action of the main constituent compounds of sorgoleone and other compounds produced by the plants roots. (AU)