Effect of lactic acid bacteria preparations on calf fecal flora

This experiment was conducted to investigate the effects of lactic acid bacteria preparations on microbial diversity and community structure of calves. On days 1 and 7 of the trial period, feces were collected into sterile tubes and labeled (Day 1: control group D1DZ, experimental group D1SY, and Day 7: control group D7DZ, experimental group D7SY). Twenty Angus calves (150±10 kg) were selected and randomly divided into two groups of 10 calves each. The control group fed a basal diet. In addition to feeding the basal diet, the experimental group was given 15 mL lactobacillus preparation orally at 09:00 and 16:00 h every day. Calves were allowed free feeding and drinking water. All other feeding environments and management conditions remained consistent with the experiment lasting for seven days. At the end of the experiment, the fecal microflora of the calves was analyzed using 16S rRNA sequencing techniques. The 16S rRNA analysis data were processed using the Excel 2007 software and analyzed by the IBM SPSS statistical software (Statistical Analysis System, version 22). The Alpha diversity index analysis showed that the Chao and the Ace indices were significantly different after feeding supplemented with lactic acid bacteria. The PCA analysis showed that the fecal flora structure differed significantly after supplementation with the lactic acid bacteria preparation. Further analysis showed that the lactic acid bacteria increased Firmicutes, Patescibacteria, Rikenellaceae_RC9_gut_group, Clostridium_sensu_stricto_1, and prevotellaceae_UCG-003 in the feces. Therefore, we speculate that lactic acid bacteria preparations play an important role in animal production and are beneficial to the diversity of the fecal microflora of the calves.

Prediction of health disorders in dairy cows monitored with collar based on binary logistic analysis / Previsão de distúrbios de saúde em vacas leiteiras monitoradas com base em coleira sobre análise logística binária

The objective of this study was to analyze data on physical activity and rumination time monitored via collars at the farm coupled with milk yield recorded by the rotary milking system to predict cows based on several disorders using the binary Logistic regression conducted with R software. Data for metritis (n=60), mastitis (n=98), lameness (n=35), and digestive disorders (n=52) were collected from 1,618 healthy cows used to construct the prediction model. To verify the feasibility and adaptability of the proposed method, we analyzed data of cows in the same herd (herd 1) not used to construct the model, and cows in another herd (herd 2) with data recorded by the same type of automated system, and led to detection of 75.0%, 64.2%, 74.2%, and 76.9% animals in herd 1 correctly predicted to suffer from metritis, mastitis, lameness, and digestive disorders, respectively. For cows in herd 2, 66.6%, 58.8%, 80.7%, and 71.4% were correctly predicted for metritis, mastitis, lameness, and digestive disorders, respectively. Compared with traditional clinical diagnoses by farm personnel, the algorithm developed allowed for earlier prediction of cows with a disorder.

Os objetivos deste estudo foram analisar dados sobre a atividade física e o tempo de ruminação monitorados através de coleiras na fazenda junto com a produção de leite registrada pelo sistema rotativo de ordenha para prever vacas com base em vários distúrbios utilizando o software de regressão logística binária realizado com o software R. Dados para metrite (n=60), mastite (n=98), manqueira (n=35) e distúrbios digestivos (n=52) foram coletados de 1.618 vacas saudáveis foram usados para construir o modelo de previsão. Para verificar a viabilidade e adaptabilidade do método proposto, analisamos os dados de vacas do mesmo rebanho (rebanho 1) não utilizadas para construir o modelo, e vacas de outro rebanho (rebanho 2) com dados registrados pelo mesmo tipo de sistema automatizado, e levamos à detecção de 75,0%, 64,2%, 74,2%, e 76,9% de animais do rebanho 1 previstos corretamente para sofrer de metrite, mastite, manqueira e distúrbios digestivos, respectivamente. Para as vacas do rebanho 2, 66,6%, 58,8%, 80,7% e 71,4% foram previstos corretamente para metrite, mastite, manqueira e distúrbios digestivos, respectivamente. Em comparação com os diagnósticos clínicos tradicionais feitos pelo pessoal da fazenda, o algoritmo desenvolvido permitiu a previsão antecipada de vacas com um distúrbio.

Marandu grass pastures with the same average height and different horizontal structures change the feeding behavior of grazing sheep? / Pastos de capim-marandu com mesma altura média e diferentes estruturas horizontais alteram o comportamento ingestivo de ovinos em pastejo?

The objective was to investigate the feeding behavior of sheep in marandu grass (Brachiaria brizantha cv. Marandu) pastures under continuous stocking with the same average height and different horizontal structures. The treatments were composed of less heterogeneous pastures (P-H, 24% coefficient of variation of plant heights and more heterogeneous pastures (P+H, 46% coefficient of variation of plant heights) and the locations of the picket, front and back, were also evaluated. A randomized block design was used, with split plots in space and four replications in two years. Crossbred lambs were used, managed under continuous stocking and variable stocking rate, to maintain average height of pastures at 30 cm. Greater masses of total forage, live leaf, live stem and dead material, and greater time of sheep in rumination, occurred in the front, in relation to the paddock background. In P-H, grazing time (GT) did not vary between regions. In P+H, GT was lower at the front than at the background. Idle time (IT) was similar between P-H and P+H. However, the animals remained more IT in the front than in the background. The feeding behavior of grazing sheep is modified in marandu grass pastures under continuous stocking with the same average height, but with different spatial variability of the vegetation.

Objetivou-se investigar o comportamento ingestivo de ovinos em pastos de capim-marandu (Brachiaria brizantha cv. Marandu) sob lotação contínua com mesma altura média e diferentes estruturas horizontais. Os tratamentos foram compostos de pastos menos heterogêneo (P-H, 24% de coeficiente de variação das alturas das plantas) e pastos mais heterogêneo (P+H, 46% de coeficiente de variação das alturas das plantas), também foi avaliado os locais do piquete, frente e fundo. O delineamento utilizado foi de blocos ao acaso, com parcelas subdivididas no espaço e quatro repetições em dois anos. Foram utilizadas borregas mestiças, manejadas sob lotação continua e com taxa de lotação variável, para manter altura média dos pastos em 30cm. Maiores massas de forragem total, folha viva, colmo vivo e material morto, e maior tempo dos ovinos em ruminação, ocorreram na frente, em relação ao fundo do piquete. No P-H, o tempo em pastejo (TP) não variou entre as regiões. No P+H, o TP foi menor na frente do que no fundo. O tempo em ócio (TO) foi semelhante entre os P-H e P+H. Porém, os animais permaneceram mais TO na frente que no fundo. O comportamento ingestivo de ovinos em pastejo é modificado em pastos de capim-marandu sob lotação contínua com mesma altura média, porém com diferentes variabilidades espaciais da vegetação.

Feeding behavior of sheep in feedlot and fed with diets containing detoxified castor cake in substitution to the soybean meal

To evaluate the ingestive behavior of sheep confined and fed with substitution levels of soybean meal (SM) by detoxified castor cake (DCC). We adopted a completely randomized design, with four levels of substitution (0; 33; 67 and 100%) with five replicates (sheep). We estimated the percentages of total times of intake of feed, time of rumination, in 'other activities', in idle agreed and in idle sleeping, dividing the day into eight periods (5h00 to 8h00; 8h01 to 11h00; 11h01 to 14h00; 14h01 to 17h00; 17h01 to 20h00; 20h01 to 23h00; 23h01 to 2h00 and 2h01 to 5h00). No interaction was observed between levels of substitution of the SM by DCC and period of the day to go. However, the isolated effect period of the day, there is more time to go from 8h00 to 11h00. The time rumination was not influenced by the levels of substitution of the SM by DCC. As for the variable other activities, the level of 67% DCC was superior to that of 100%. Regarding the variables idle agreed and idle sleeping, were not observed effects of substitution levels of SM by DCC. The DCC provides no changes in the behavior of the sheep, but the period of the day exerts influence on the behavioral pattern of such animals.(AU)

Influence of pasture, silvopastoral, and feedlot production systems and the recording interval of behavioral activities on the ingestive behavior of lambs / Influência dos sistemas de produção em pastagem, silvipastoril e confinamento, e do intervalo de registro das atividades comportamentais no comportamento ingestivo de cordeiros

The evaluation of the ingestive behavior of ruminant animals is important to identify the animal response to the particularities of the different production systems. However, the five-minute evaluation technique requires personnel, making it difficult to adhere to farms and research in more complex pastoral systems. Thus, this study objected to evaluating the influence of pasture, silvopastoral, and feedlot production systems on the ingestive behavior of lambs and to defining the behavior recording interval in each of the systems. Twenty-four lambs were distributed in an experimental design in split plots. The plots were the production systems (pasture without shading - PS, silvopastoral - SS, and feedlot systems - FS) and the subplots were the behavior observation intervals (5, 10, 15, and 20 min.). In the pasture without shading and silvopastoral systems, the lambs were kept on Aruana grass (Megathyrsus maximus) pasture and supplemented with concentrate at 1.5% of body weight per day, on a dry matter (DM) basis. In the feedlot system, the lambs received a diet consisting of 200 g kg DM-1 of annual ryegrass hay (Lolium multiflorum Lam.) and 800 g kg DM-1 of concentrate. There was no interaction (P > 0.05) between the production system and the recording interval of behavioral activities. The time spent on food intake was higher (P < 0.05) in the pasture (533 min d-1) and silvopastoral (513 min d-1) systems than in the feedlot (225 min d-1). In contrast, for water intake, time was higher (P < 0.05) in the feedlot (21 min d-1) and lower in the silvopastoral system (5 min d-1). There was no difference (P > 0.05) between the systems for rumination and idle activities, of which daily averages were 378 and 587 min d-1, respectively. There was also no difference (P > 0.05) between the observation intervals for feed, water, rumination, and idleness activities. Lambs in pasture-based systems spend more time feeding, and feedlot lambs spend more time ingesting water. Feedlot lambs ruminate as much as grazing animals when fed hay, even though hay represents only 20% of the total diet. The recording interval of behavioral activities in these production systems can be up to 20 minutes.(AU)

A avaliação do comportamento ingestivo de animais ruminantes é importante para identificar a resposta animal frente às particularidades dos diferentes sistemas de produção. Entretanto, a técnica de avaliação usualmente utilizada, com intervalos de cinco minutos, requer mão de obra, dificultando sua adesão em propriedades e em pesquisas em sistemas pastoris de maior complexidade. Assim, este estudo foi realizado com os objetivos de avaliar a influência dos sistemas de produção em pastagem, silvipastoril e confinamento sobre o comportamento ingestivo de cordeiros e definir o intervalo de registro do comportamento em cada um dos sistemas. Foram utilizados 24 cordeiros distribuídos em delineamento experimental em parcelas subdivididas, sendo as parcelas os sistemas de produção (pastagem sem sombreamento, sistema silvipastoril e confinamento) e as subparcelas os intervalos de observação do comportamento (5, 10, 15 e 20 min.). Nos sistemas em pastagem sem sombreamento e silvipastoril, os cordeiros permaneceram em pastagem de capim Aruana (Megathyrsus maximus) e foram suplementados com concentrado a 1,5% do peso corporal ao dia em base de matéria seca (MS). No sistema em confinamento, os cordeiros receberam ração composta por 200 g kg MS-1 de feno de azevém anual (Lolium multiflorum Lam.) e 800 g kg MS-1 de concentrado. Não houve interação (P > 0,05) entre sistema de produção e intervalo de registro das atividades comportamentais. O tempo despendido para a ingestão de alimento foi maior (P < 0,05) no sistema de pastagem (533 min d-1) e silvipastoril (513 min d-1) em relação ao confinamento (225 min d-1), enquanto que para ingestão de água foi maior (P < 0,05) no confinamento (21 min d-1) e menor no silvipastoril (5 min d-1). Não houve diferença (P > 0,05) entre os sistemas para as atividades de ruminação e ócio, cujas médias diárias foram de 378 e 587 min d-1, respectivamente. Também não houve diferença (P > 0,05) entre os intervalos de observação para as atividades de ingestão de alimento, água, ruminação e ócio. Cordeiros em sistemas à base de pastagem gastam mais tempo se alimentando, e cordeiros confinados mais tempo ingerindo água. Cordeiros confinados ruminam tanto quanto animais em pastejo quando alimentados com feno, mesmo que este represente apenas 20% da dieta total. O intervalo de registro das atividades comportamentais nestes sistemas de produção pode ser de até 20 minutos.(AU)

Babassu mesocarp meal for ewe lambs feeding: In vitro ruminal fermentation and in vivo apparent digestibility

The purpose of the current study was to evaluate the in vitro fermentation (IVRF) and apparent in vivo digestibility (AIVD) of diets formulated with four inclusion levels of babassu mesocarp meal (BMM) (0.0; 7.5; 15.0 and 22.5%) in ewe lambs. The IVRF test was performed through the "Hohenheim Gas Test" technique, following a randomized complete block design, with four blocks (rumen fluids from four different bovines). Gas production was measured at 3, 6, 9, 12, 24, 48, 72, and 96 hours after incubation. For the AIVD trial, 20 ewe lambs were used in a completely randomized design with five replicates. The animals were housed in metabolic cages during the digestibility test. Diets with BMM showed lower fermentation levels than those without BMM. The BMM reduced the gas production per incubation time and dry matter effective degradability (DMED), possibly due to its chemical characteristics, or even to the influence of other factors, such as physical properties. The BMM negatively influenced the AIVD of DM. Thus, it can be stated that, despite being a starch source, BMM reduces ruminal fermentation parameters when included in ruminant diets, negatively interfering with diet digestibility. Therefore, its use should be cautious.

Babassu mesocarp meal for ewe lambs feeding: In vitro ruminal fermentation and in vivo apparent digestibility

The purpose of the current study was to evaluate the in vitro fermentation (IVRF) and apparent in vivo digestibility (AIVD) of diets formulated with four inclusion levels of babassu mesocarp meal (BMM) (0.0; 7.5; 15.0 and 22.5%) in ewe lambs. The IVRF test was performed through the "Hohenheim Gas Test" technique, following a randomized complete block design, with four blocks (rumen fluids from four different bovines). Gas production was measured at 3, 6, 9, 12, 24, 48, 72, and 96 hours after incubation. For the AIVD trial, 20 ewe lambs were used in a completely randomized design with five replicates. The animals were housed in metabolic cages during the digestibility test. Diets with BMM showed lower fermentation levels than those without BMM. The BMM reduced the gas production per incubation time and dry matter effective degradability (DMED), possibly due to its chemical characteristics, or even to the influence of other factors, such as physical properties. The BMM negatively influenced the AIVD of DM. Thus, it can be stated that, despite being a starch source, BMM reduces ruminal fermentation parameters when included in ruminant diets, negatively interfering with diet digestibility. Therefore, its use should be cautious.(AU)

Use of the rumination profile through collar sensors for mastitis diagnosis in dairy cows

Background: Mastitis is an inflammatory disease of the mammary gland, mostly associated with bacterial infections. Itis responsible for great economic losses due to decreased milk yield, discarded milk, milk composition alterations andtreatment costs, besides it impairs the animal health and welfare. The rumination time is an important behavioral markerand its assessment can be used as an early diagnosis tool, which can improve cure rate. Therefore, the aim of the presentstudy was to evaluate the sensitivity of behavior monitoring system collars in the diagnosis of mastitis and the averagerumination time (RT) of Holstein cows during the healthy period and affected by the disease.Materials, Methods & Results: The study was conducted on a commercial property located in the municipality of RioGrande, Rio Grande do Sul, Brazil. The RT data from 39 multiparous Holstein cows with an average milk yield of 38.4 L/day was collected. RT monitoring was performed using C-Tech1 collars combined with CowMed® software, which assessbehavior data from the animals and emits warning signals when it finds abnormalities in any parameter. In order to verifywhether the animals were determined correlated with diseases, the sensitivity of the data was evaluated, when the systemhad given the alert to animals considered ill, they underwent to a further clinical evaluation performed by a veterinarian toconfirm the diagnosis. From the diagnosis, the cows were divided into subclinical mastitis (SM) and clinical mastitis (CM)groups. SM was detected by the California Mastitis Test (CMT) and cows that were graded 1 (++), 2 (++) or 3 (+++) withoutthe presence of any other clinical sign were assigned to the SM group. CM was assessed by observation of abnormalitiesin milk such as changes in color and consistency, as well as the presence of lumps, clots or blood; and clinical examinationof the udder was performed for detection of hot, hard, swollen or painful quarters...

Use of the rumination profile through collar sensors for mastitis diagnosis in dairy cows

Background: Mastitis is an inflammatory disease of the mammary gland, mostly associated with bacterial infections. Itis responsible for great economic losses due to decreased milk yield, discarded milk, milk composition alterations andtreatment costs, besides it impairs the animal health and welfare. The rumination time is an important behavioral markerand its assessment can be used as an early diagnosis tool, which can improve cure rate. Therefore, the aim of the presentstudy was to evaluate the sensitivity of behavior monitoring system collars in the diagnosis of mastitis and the averagerumination time (RT) of Holstein cows during the healthy period and affected by the disease.Materials, Methods & Results: The study was conducted on a commercial property located in the municipality of RioGrande, Rio Grande do Sul, Brazil. The RT data from 39 multiparous Holstein cows with an average milk yield of 38.4 L/day was collected. RT monitoring was performed using C-Tech1 collars combined with CowMed® software, which assessbehavior data from the animals and emits warning signals when it finds abnormalities in any parameter. In order to verifywhether the animals were determined correlated with diseases, the sensitivity of the data was evaluated, when the systemhad given the alert to animals considered ill, they underwent to a further clinical evaluation performed by a veterinarian toconfirm the diagnosis. From the diagnosis, the cows were divided into subclinical mastitis (SM) and clinical mastitis (CM)groups. SM was detected by the California Mastitis Test (CMT) and cows that were graded 1 (++), 2 (++) or 3 (+++) withoutthe presence of any other clinical sign were assigned to the SM group. CM was assessed by observation of abnormalitiesin milk such as changes in color and consistency, as well as the presence of lumps, clots or blood; and clinical examinationof the udder was performed for detection of hot, hard, swollen or painful quarters...(AU)

Subproduct of the Wine Industry to Replace Forage for Feeding Confined Ovine / Coproduto da Indústria de Vitivinificação como Substituto ao Volumoso na Alimentação de Ovinos Confinados

The aim of the present study was to evaluate the ingestive behavior of sheep receiving alternative food with grape pomace to replace roughage, as well as its impacts on the rumen environment. It was observed that BU had higher TOC, TRAM and pH of the ruminal liquid, while there was less TRU (P < 0,05). As for the other parameters, no significant differences were found. In this study, just as the feeding time was not influenced due to the similarity of the concentrate: roughage ratio in the diet, the protozoan count did not change. This finding reinforces the possibility of using grape marc as a tool for formulating feed, reducing the risk of ruminal disturbances. It was observed that the group control had lower TRAM, indicating that the diet of this group promoted greater microbial activity and, consequently, greater digestibility, corroborating the already observed TOC. This result was the opposite of what was expected, since the grape pomace has probiotic properties, precisely because the fruits have more fungi and bacteria in their microbiota. This also confirms that the co-products of vitiviniculture can act as promoters of the intestinal health of ruminants, justifying further studies in order to improve this use. Therefore, the use of wine by-products is an alternative to reduce production costs, as its use in diets for confined sheep can improve the performance of the animals, increasing the total feed consumption and improving microbial activity.(AU)

O objetivo do presente estudo foi avaliar o comportamento ingestivo de ovinos recebendo ração alternativa com bagaço de uva em substituição ao volumoso, bem como seus impactos no ambiente ruminal. Observou-se que a BU apresentou maiores COT, TRAM e pH do líquido ruminal, enquanto houve menor TRU (P <0,05). Quanto aos demais parâmetros, não foram encontradas diferenças significativas. Neste estudo, assim como o tempo de alimentação não foi influenciado pela similaridade da relação concentrado: volumoso na dieta, a contagem de protozoários não se alterou. Esse achado reforça a possibilidade do uso do bagaço de uva como ferramenta na formulação de rações, reduzindo o risco de distúrbios ruminais. Observou-se que o grupo controle apresentou menor TRAM, indicando que a dieta desse grupo promoveu maior atividade microbiana e, consequentemente, maior digestibilidade, corroborando o COT já observado. Esse resultado foi o oposto do esperado, já que o bagaço de uva tem propriedades probióticas, justamente porque os frutos possuem mais fungos e bactérias em sua microbiota. Isso também confirma que os coprodutos da vitivinicultura podem atuar como promotores da saúde intestinal de ruminantes, justificando novos estudos no sentido de aprimorar esse uso. Portanto, a utilização de subprodutos do vinho é uma alternativa para reduzir os custos de produção, pois seu uso em dietas para ovinos confinados pode melhorar o desempenho dos animais, aumentando o consumo total de ração e melhorando a atividade microbiana.(AU)

Subproduct of the Wine Industry to Replace Forage for Feeding Confined Ovine

ABSTRACT The aim of the present study was to evaluate the ingestive behavior of sheep receiving alternative food with grape pomace to replace roughage, as well as its impacts on the rumen environment. It was observed that BU had higher TOC, TRAM and pH of the ruminal liquid, while there was less TRU (P 0,05). As for the other parameters, no significant differences were found. In this study, just as the feeding time was not influenced due to the similarity of the concentrate: roughage ratio in the diet, the protozoan count did not change. This finding reinforces the possibility of using grape marc as a tool for formulating feed, reducing the risk of ruminal disturbances. It was observed that the group control had lower TRAM, indicating that the diet of this group promoted greater microbial activity and, consequently, greater digestibility, corroborating the already observed TOC. This result was the opposite of what was expected, since the grape pomace has probiotic properties, precisely because the fruits have more fungi and bacteria in their microbiota. This also confirms that the co-products of vitiviniculture can act as promoters of the intestinal health of ruminants, justifying further studies in order to improve this use. Therefore, the use of wine by-products is an alternative to reduce production costs, as its use in diets for confined sheep can improve the performance of the animals, increasing the total feed consumption and improving microbial activity.

RESUMO O objetivo do presente estudo foi avaliar o comportamento ingestivo de ovinos recebendo ração alternativa com bagaço de uva em substituição ao volumoso, bem como seus impactos no ambiente ruminal. Observou-se que a BU apresentou maiores COT, TRAM e pH do líquido ruminal, enquanto houve menor TRU (P 0,05). Quanto aos demais parâmetros, não foram encontradas diferenças significativas. Neste estudo, assim como o tempo de alimentação não foi influenciado pela similaridade da relação concentrado: volumoso na dieta, a contagem de protozoários não se alterou. Esse achado reforça a possibilidade do uso do bagaço de uva como ferramenta na formulação de rações, reduzindo o risco de distúrbios ruminais. Observou-se que o grupo controle apresentou menor TRAM, indicando que a dieta desse grupo promoveu maior atividade microbiana e, consequentemente, maior digestibilidade, corroborando o COT já observado. Esse resultado foi o oposto do esperado, já que o bagaço de uva tem propriedades probióticas, justamente porque os frutos possuem mais fungos e bactérias em sua microbiota. Isso também confirma que os coprodutos da vitivinicultura podem atuar como promotores da saúde intestinal de ruminantes, justificando novos estudos no sentido de aprimorar esse uso. Portanto, a utilização de subprodutos do vinho é uma alternativa para reduzir os custos de produção, pois seu uso em dietas para ovinos confinados pode melhorar o desempenho dos animais, aumentando o consumo total de ração e melhorando a atividade microbiana.

Which non-fibrous carbohydrates: neutral detergent fiber ratio optimizes the use of cactus cladodes in diets of growing heifers? / Qual relação carboidratos não fibrosos: fibra em detergente neutro otimiza o uso de palma forrageira em dietas para novilhas em crescimento?

This study aimed to evaluate the effect of different non-fibrous carbohydrate (NFC):neutral detergent fiber (NDF) ratios (0.70:1, 0.90:1, 1.14:1, 1.42:1, and 1.85:1) in diets containing cactus cladodes on intake, digestibility, ruminal parameters, ingestive behavior, and microbial protein of growing heifers. Five rumencannulated heifers (194 ± 70 kg of body weight) were distributed in a Latin square design (5 x 5). There was a quadratic effect on the intake of dry matter (DM) (Kg day-1 and %BW) and crude protein (CP) with the increase in non-fibrous carbohydrate (NFC): neutral detergent fiber (NDF) ratio, with maximum values of 7.07 kg day-1, 2.86%, and 0.949 kg day-1, estimated for 1.37:1, 1.39:1, and 1.27:1 ratios. When the intake of NDF decreased, in turn, the intakes of NFC and TDN increased. The NDF digestibility decreased with increasing NFC levels in diets. The other nutrients were not influenced. The ruminal pH showed the lowest values at 4 hours after feeding, mainly for animals fed the 1.85:1 ratio. There was a quadratic effect for rumination time with maximum values estimated for an NFC:NDF ratio of 0.88:1. The time spent idle increased linearly, while total chewing time and feeding efficiency of NDF decreased. The urinary volume increased linearly. However, microbial protein synthesis and the efficiency of the synthesis were not influenced. The number of protozoa increased by 64.5% with the elevation of the NFC:NDF ratio. We recommend a NFC:NDF ratio of 1.37:1 in diets containing cactus cladodes, to improve the use of nutrients, without negatively affecting ruminal parameters.(AU)

Este estudo teve como objetivo avaliar o efeito de diferentes relações carboidratos não fibrosos (CNF): fibra em detergente neutro (FDN) (0,70: 1; 0,90: 1; 1,14: 1; 1,42: 1 e 1,85: 1) em dietas contendo palma forrageira sobre o consumo, digestibilidade, parâmetros ruminais, comportamento ingestivo e proteína microbiana de novilhas em crescimento. Cinco novilhas, fistuladas no rúmen (194 ± 70 kg de peso corporal) foram distribuídas em um delineamento em quadrado latino (5 x 5). Houve efeito quadrático sobre o consumo de matéria seca (MS) (kg dia-1 (% PC) e proteína bruta (PB) com o aumento da relação carboidratos não fibrosos (CNF): fibra em detergente neutro (FDN), com valores máximos de 7,07 kg dia-1, 2,86% e 0,949 kg dia-1, estimado para 1,37: 1; 1,39: 1 e proporção de 1,27: 1. Enquanto a ingestão de FDN diminuiu, a ingestão de CNF e NDT aumentou. A digestibilidade da FDN diminuiu com o aumento dos teores de FDN nas dietas. Os demais nutrientes não foram influenciados. O pH ruminal apresentou os menores valores 4 horas após a alimentação, principalmente para os animais alimentados na proporção de 1,85: 1. Houve efeito quadrático para o tempo de ruminação com valores máximos estimados para uma relação CNF: FDN de 0,88: 1. O tempo de ócio aumentou linearmente, enquanto o tempo total de mastigação e a eficiência alimentar da FDN diminuíram. O volume urinário aumentou linearmente. No entanto, a síntese de proteína microbiana e a eficiência da síntese não foram influenciadas. O número de protozoários aumentou 64,5% com a elevação da relação CNF: FDN. Recomenda-se a relação CNF:FDN de 1,37: 1 em dietas contendo palma forrageira, para melhorar o aproveitamento dos nutrientes, sem afetar negativamente os parâmetros ruminais.(AU)

Selectivity and feeding behavior of Saanen goats subjected to three nutritional levels

This study was conducted to investigate the effect of selective feeding habits on the quality of the ingested diet and the effect of feed restriction on the selectivity and behavior of goats in feedlots. Differences were found only in the amount of feed given to the animals, with a difference in the amount of feed rejected as a function of the nutritional level. Higher levels of orts were measured for those animals that received the ad libitum diet. For the composition of orts, differences were measured only in the ether extract fraction. Animals fed the ad libitum diet increased their intake of ether extract and energy, crude fiber, neutral detergent fiber, and acid detergent fiber. Thus, they had a preference for consuming the fibrous and energetic portions of the diet to the detriment of the mineral matter. The granulometry influenced the leftovers for the male and female treatments only in the diet that contained the 2.00- and 0.063-mm sized particles, with larger leftovers for the females. There were statistical differences as a function of feeding level for time spent in rumination, with stereotypical behavior, and in time spent standing up or lying down. As a function of sex, the differences were the same variables, including the time spent with agonistic behavior. Animals that received the ad libitum diet had a greater gain and final weight, whereas the animals that received the 60% restricted diet had the lowest performance, as did the females. Feed restriction changed the natural feeding behavior of goats, with smaller feed particles preferred. The feed restriction and sex also influenced the time spent on nonproductive activities, and the animals became more restless, with females having a higher social behavior than males.(AU)

Consumption and ingestive behaviour of lambs fed varying levels of babassu mesocarp bran / Farelo do mesocarpo do babaçu na alimentação de borregas: consumo e comportamento ingestivo

Voluntary consumption (VolC) and ingestive behaviour (IB) of lambs fed diets containing different percentages (0.0; 7.5; 15.0 and 22.5%) of babassu mesocarp bran (I-BMB) were evaluated. The trial was at the Federal University of Tocantins, Campus de Araguaína-TO. Twenty lambs were evaluated using a completely randomized design, and five replications were performed. VolC data was obtainedby measuring total volumes of both food supplied and food remaining after feeding. IB was evaluated throughout two, non-consecutive 24-h periods, with observations were recorded every five minutes that described the following behavioural variables: resting (REST), rumination (RUM), intake (INT) and chewing (CHEW). The particle size was also measured using a Penn State particle separator. All VolC parameters (dry matter intake, DMI; crude protein intake, CPI; neutral detergent fibre intake, NDFI; and acid detergent fibre intake, ADFI) increased linearly with I-BMB intake. I-BMB consumption did not interfere with water intake, nor did it affect I-BMB did not affect the duration of DMI, NDFI and ADFI. However, linear increases of rumination and chewing activities associated with DMI, NDFI and ADFI were observed as I-BMB levels increased. In summary, effects of inclusion I-BMB in diets of lambs included increased voluntary consumption, and reduced rumination and chewing, but not ingestion, efficiency. When economically favorable, these data suggest that inclusion of up to 22.5% I-BMB within balanced diets should be applied.

Avaliou-se o consumo voluntário (CVol) e o comportamento ingestivo (CI) de borregas alimentadas com rações contendo diferentes inclusões de farelo do mesocarpo do babaçu (I-FMB) (0,0; 7,5; 15,0 e 22,5%). O local do ensaio foi a ovinocaprinocultura da Universidade Federal do Tocantins, Campus de Araguaína-TO. Foram utilizadas 20 borregas distribuídas em delineamento inteiramente casualisado, com cinco repetições. O CVol foi realizado pela mensuração do total de alimento fornecido e das sobras. Já o CI foi avaliado em dois períodos de 24 horas em dias não consecutivos, com observações de cinco em cinco minutos sendo as variáveis comportamentais: ócio (O), ruminação (R), ingestão (I)e mastigação (M). Mensurou-se também o tamanho de partícula com separador de partículas modelo Penn State. Foi observado aumento linear crescente com a I-FMB para todos os parâmetros de CVol estudados (consumo de matéria seca - CMS, consumo de proteína bruta - CPB, consumo de fibra em detergente neutro - CFND e consumo de fibra em detergente ácido - CFDA) em todas as unidades (gdia-1 animal-1, % do peso vivo (PV), g kg-0,75). A I-FMB não interferiu na ingestão de água de borregas. A I-FMB não afetou o tempo em ingestão por CMS, CFDN e CFDA. No entanto, a I-FMB aumentou linearmente as atividades ruminação e mastigação em função do CMS, CFDN e CFDA. A I-FMB na dieta de borregas aumenta o consumo voluntário de ovinos e influência de forma negativa na eficiência de ruminação e mastigação, sem prejudicar eficiência de ingestão. Quando economicamente favorável recomenda-se a inclusão de até 22,5 % de FMB em dietas balanceadas.

Feeding behavior of goat kids fed diets containing peach palm meal

The aim in this study was to evaluate the feeding behavior of goat kids fed diets containing peach palm meal replacing corn (0, 10, 40, 60, and 85 % on a dry matter basis). Thirty crossbred Boer kids, with 90 days old and initial body weight of 16.7 ± 3.5 kg were distributed in a completely randomized design with six replicates. Diets were daily supplied ad libitum to allow 10-20 % leftovers. Three collections were performed every 28 days. Diets were isoenergetics and isonitrogenous, composed of corn, soybean meal, peach palm meal, mineral supplement and Tifton 85 hay, with roughage: concentrate ratio of 30:70. There was a linear reduction in the intakes of dry matter (DM) and neutral detergent fiber (NDF) with a respective decrease of 35.7 % and 58.8 % comparing the diet with 85 % replacement and the control diet. The rumination and chewing times increased in diets with peach palm meal. The feeding and rumination rates decreased with reducing intakes of DM and NDF. The peach palm meal affects the feeding behavior and decreases the feed intake in response to palatability, fat and fiber composition, and it is recommended until 10 % replacing corn.(AU)

Consumption and ingestive behaviour of lambs fed varying levels of babassu mesocarp bran / Farelo do mesocarpo do babaçu na alimentação de borregas: consumo e comportamento ingestivo

Voluntary consumption (VolC) and ingestive behaviour (IB) of lambs fed diets containing different percentages (0.0; 7.5; 15.0 and 22.5%) of babassu mesocarp bran (I-BMB) were evaluated. The trial was at the Federal University of Tocantins, Campus de Araguaína-TO. Twenty lambs were evaluated using a completely randomized design, and five replications were performed. VolC data was obtainedby measuring total volumes of both food supplied and food remaining after feeding. IB was evaluated throughout two, non-consecutive 24-h periods, with observations were recorded every five minutes that described the following behavioural variables: resting (REST), rumination (RUM), intake (INT) and chewing (CHEW). The particle size was also measured using a Penn State particle separator. All VolC parameters (dry matter intake, DMI; crude protein intake, CPI; neutral detergent fibre intake, NDFI; and acid detergent fibre intake, ADFI) increased linearly with I-BMB intake. I-BMB consumption did not interfere with water intake, nor did it affect I-BMB did not affect the duration of DMI, NDFI and ADFI. However, linear increases of rumination and chewing activities associated with DMI, NDFI and ADFI were observed as I-BMB levels increased. In summary, effects of inclusion I-BMB in diets of lambs included increased voluntary consumption, and reduced rumination and chewing, but not ingestion, efficiency. When economically favorable, these data suggest that inclusion of up to 22.5% I-BMB within balanced diets should be applied.(AU)

Avaliou-se o consumo voluntário (CVol) e o comportamento ingestivo (CI) de borregas alimentadas com rações contendo diferentes inclusões de farelo do mesocarpo do babaçu (I-FMB) (0,0; 7,5; 15,0 e 22,5%). O local do ensaio foi a ovinocaprinocultura da Universidade Federal do Tocantins, Campus de Araguaína-TO. Foram utilizadas 20 borregas distribuídas em delineamento inteiramente casualisado, com cinco repetições. O CVol foi realizado pela mensuração do total de alimento fornecido e das sobras. Já o CI foi avaliado em dois períodos de 24 horas em dias não consecutivos, com observações de cinco em cinco minutos sendo as variáveis comportamentais: ócio (O), ruminação (R), ingestão (I)e mastigação (M). Mensurou-se também o tamanho de partícula com separador de partículas modelo Penn State. Foi observado aumento linear crescente com a I-FMB para todos os parâmetros de CVol estudados (consumo de matéria seca - CMS, consumo de proteína bruta - CPB, consumo de fibra em detergente neutro - CFND e consumo de fibra em detergente ácido - CFDA) em todas as unidades (gdia-1 animal-1, % do peso vivo (PV), g kg-0,75). A I-FMB não interferiu na ingestão de água de borregas. A I-FMB não afetou o tempo em ingestão por CMS, CFDN e CFDA. No entanto, a I-FMB aumentou linearmente as atividades ruminação e mastigação em função do CMS, CFDN e CFDA. A I-FMB na dieta de borregas aumenta o consumo voluntário de ovinos e influência de forma negativa na eficiência de ruminação e mastigação, sem prejudicar eficiência de ingestão. Quando economicamente favorável recomenda-se a inclusão de até 22,5 % de FMB em dietas balanceadas.(AU)

Ingestive behavior, ruminal and blood kinetics in lambs fed cow cheese whey / Comportamento ingestivo, cinéticas ruminal e sanguínea em ovinos alimentados com soro de queijo bovino

The objective of this study was to evaluate the inclusion of cow cheese whey (CCW) in the diet for lambs on the ingestive behavior, ruminal and blood parameters. Twenty crossbred, male, non-castrated lambs with 25 kg body weight were assigned to four treatments: control diet (composed of corn, soybean meal and native pasture silage) and test diets (control diet + inclusion of CCW in increasing levels of 1.6, 2.7 and 4.0% in DM), in a completely randomized design with four treatments and five replications. Means were compared by Tukeys test, and the strength of association between variables was tested by Pearsons correlation. Regression analysis was also performed for data from the collection of ruminal fluid and blood. Behavioral assessments were made at a 24-hour interval in measurements taken every five minutes. The inclusion of CCW in the diet did not impair ruminal pH, but promoted a decrease in rumination and feeding times, also causing a lower number of cuds and a lower number of cud chews. The lowest concentration of ammonia nitrogen and total protein were found in lambs receiving the diet with 4% CCW. However, these values were within the range recommended for microbial growth. CCW is a potential protein alternative in lambs feed as it does not interfere with nutrient intake, ruminal and blood parameters, and favors a positive nitrogen balance.(AU)

Objetivou-se avaliar a inclusão de soro de queijo bovino (SQB) na dieta para ovinos sobre o comportamento ingestivo, os parâmetros ruminais e sanguíneos. Vinte ovinos mestiços, machos, não castrados, com 25 kg de peso corporal foram distribuídos em quatro dietas: dieta controle (composta por milho, farelo de soja e silagem de pasto nativo) e as dietas teste (dieta controle + inclusão de SQB em níveis crescentes 1,6; 2,7 e 4,0% na MS), em um delineamento inteiramente ao acaso com quatro tratamentos e cinco repetições. A comparação de médias foi realizada pelo teste Tukey e foram testadas as correlações de Pearson entre as variáveis. Também foi feita a análise de regressão para os dados provenientes da coleta de líquido ruminal e de sangue. As avaliações de comportamento foram feitas em um intervalo de 24 horas em medições realizadas a cada cinco minutos. A inclusão de SQB na dieta não comprometeu o pH ruminal, mas promoveu a queda nos tempos de ruminação e de alimentação, ocasionando também menor número de bolos ruminais e menor número de mastigações merícicas. A menor concentração de nitrogênio amoniacal e proteínas totais foram encontradas nos ovinos que receberam a dieta com 4% de inclusão de SQB. Entretanto, esses valores ficaram dentro do padrão estabelecido para crescimento microbiano. O SQB é uma potencial alternativa proteica na alimentação de ovinos por não interferir no consumo de nutrientes, nos parâmetros ruminais e sanguíneos, e favorecer o balanço positivo de nitrogênio.(AU)

Feeding behavior of goat kids fed diets containing peach palm meal

The aim in this study was to evaluate the feeding behavior of goat kids fed diets containing peach palm meal replacing corn (0, 10, 40, 60, and 85 % on a dry matter basis). Thirty crossbred Boer kids, with 90 days old and initial body weight of 16.7 ± 3.5 kg were distributed in a completely randomized design with six replicates. Diets were daily supplied ad libitum to allow 10-20 % leftovers. Three collections were performed every 28 days. Diets were isoenergetics and isonitrogenous, composed of corn, soybean meal, peach palm meal, mineral supplement and Tifton 85 hay, with roughage: concentrate ratio of 30:70. There was a linear reduction in the intakes of dry matter (DM) and neutral detergent fiber (NDF) with a respective decrease of 35.7 % and 58.8 % comparing the diet with 85 % replacement and the control diet. The rumination and chewing times increased in diets with peach palm meal. The feeding and rumination rates decreased with reducing intakes of DM and NDF. The peach palm meal affects the feeding behavior and decreases the feed intake in response to palatability, fat and fiber composition, and it is recommended until 10 % replacing corn.

Ingestive behavior, ruminal and blood kinetics in lambs fed cow cheese whey / Comportamento ingestivo, cinéticas ruminal e sanguínea em ovinos alimentados com soro de queijo bovino

The objective of this study was to evaluate the inclusion of cow cheese whey (CCW) in the diet for lambs on the ingestive behavior, ruminal and blood parameters. Twenty crossbred, male, non-castrated lambs with 25 kg body weight were assigned to four treatments: control diet (composed of corn, soybean meal and native pasture silage) and test diets (control diet + inclusion of CCW in increasing levels of 1.6, 2.7 and 4.0% in DM), in a completely randomized design with four treatments and five replications. Means were compared by Tukeys test, and the strength of association between variables was tested by Pearsons correlation. Regression analysis was also performed for data from the collection of ruminal fluid and blood. Behavioral assessments were made at a 24-hour interval in measurements taken every five minutes. The inclusion of CCW in the diet did not impair ruminal pH, but promoted a decrease in rumination and feeding times, also causing a lower number of cuds and a lower number of cud chews. The lowest concentration of ammonia nitrogen and total protein were found in lambs receiving the diet with 4% CCW. However, these values were within the range recommended for microbial growth. CCW is a potential protein alternative in lambs feed as it does not interfere with nutrient intake, ruminal and blood parameters, and favors a positive nitrogen balance.

Objetivou-se avaliar a inclusão de soro de queijo bovino (SQB) na dieta para ovinos sobre o comportamento ingestivo, os parâmetros ruminais e sanguíneos. Vinte ovinos mestiços, machos, não castrados, com 25 kg de peso corporal foram distribuídos em quatro dietas: dieta controle (composta por milho, farelo de soja e silagem de pasto nativo) e as dietas teste (dieta controle + inclusão de SQB em níveis crescentes 1,6; 2,7 e 4,0% na MS), em um delineamento inteiramente ao acaso com quatro tratamentos e cinco repetições. A comparação de médias foi realizada pelo teste Tukey e foram testadas as correlações de Pearson entre as variáveis. Também foi feita a análise de regressão para os dados provenientes da coleta de líquido ruminal e de sangue. As avaliações de comportamento foram feitas em um intervalo de 24 horas em medições realizadas a cada cinco minutos. A inclusão de SQB na dieta não comprometeu o pH ruminal, mas promoveu a queda nos tempos de ruminação e de alimentação, ocasionando também menor número de bolos ruminais e menor número de mastigações merícicas. A menor concentração de nitrogênio amoniacal e proteínas totais foram encontradas nos ovinos que receberam a dieta com 4% de inclusão de SQB. Entretanto, esses valores ficaram dentro do padrão estabelecido para crescimento microbiano. O SQB é uma potencial alternativa proteica na alimentação de ovinos por não interferir no consumo de nutrientes, nos parâmetros ruminais e sanguíneos, e favorecer o balanço positivo de nitrogênio.

Protein supplementation for sheep fed with tropical forage / Suplementação proteica para ovinos alimentados com forragem tropical

The effects of increasing levels of protein supplementation (50, 100, 150 and 200 animal per day) and mineral salt on feed intake, nutrient digestibility and ingestive behavior of sheep consuming tropical forage (Pennisetum purpureum Schum) were evaluated. Five non-castrated crossbred lambs (½ Santa Inês x ½ undefined breed) with average initial body weight of 35.0 kg (± 4.40 kg) were allocated in individual cages and analyzed in a 5x5 Latin square experimental design composed of five animals, five treatments and five evaluation periods of 14 days each. The contrast (protein supplement vs. mineral mixture) was not significant (p>0.05) for total dry matter intake, total organic matter intake, total ether extract intake and total carbohydrate intake variables. However, it was significant (p 0.05) either the water intake or ingestive behavior of sheep, with rumination and rest coinciding with dusk and dawn.

Foram avaliados os efeitos de níveis crescentes de suplemento proteico (50, 100, 150 e 200 gramas/animal dia-1) e do suplemento testemunha (mistura mineral) sobre o consumo, a digestibilidade dos nutrientes e o comportamento ingestivo de ovinos consumindo forragem tropical (Pennisetum purpureum Schum). Foram utilizados cinco borregos meio sangue Santa Inês x Sem Raça Definida, não castrados, com peso corporal inicial médio de 25,33 kg (± 4,40 kg), alocados em baias individuais e distribuídos em delineamento experimental quadrado latino 5x5, sendo cinco animais, cinco tratamentos e cinco períodos de avaliação de 14 dias cada. Observou-se que para as variáveis consumo de matéria seca total, consumo de matéria orgânica total, consumo de extrato etéreo total e consumo de carboidratos totais total, o contraste (suplemento proteico x mistura mineral) não foi significativo (p>0,05), contudo, este foi significativo (p0,05), portanto, mesmo no nível mais elevado de fornecimento de suplemento (200 g/dia) os animais não consumiram mais água pelo maior aporte proteico do mesmo. Concluiu-se que os níveis crescentes de suplementação proteica associados ao capim Elefante (Pennisetum purpureum Schum) de boa qualidade promovem incrementos nos parâmetros digestivos dos ovinos, contudo, a oferta de suplemento abaixo de 100 g/dia resulta em parâmetros semelhantes aos da mistura mineral. Adicionalmente, a suplementação proteica não afeta (P>0,05) a ingestão de água dos ovinos e o seu comportamento ingestivo, com ruminação e ócio coincidindo com o anoitecer e o amanhecer.