Morphological aspects of epididymal microscopy and rete testis in greater Rhea Americana / Aspectos morfológicos da microscopia do epitélio do epidídimo da Rhea americana

The purpose of this research was to study the histology and describe the microscopy findings of the epididymis epithelium of greater Rhea americana at three time periods: November 2005 (n=14), December 2006 (n= 20), and May 2007 (n= 20), to observe and compare the differences that occurred. We studied the epididymis from 54 rheas, bred in Santa Maria, Rio Grande do Sul, Brazil. The epididymis were collected during commercial slaughter and fixed in bouin. Optical microscopy was used to measure the cellular structure, types of cells, tubules, and stereological values like the epididymis epithelium diameters, lumen, thickness, and relative volume of the tissue structure. Additionally, electron microscopy was studied. In December 2006 and May 2007, the means of the epididymis tubular diameter were: 79.1 and 58.1 µm, epithelium thickness: 24.0 and 52.2 µm, and lumen diameter: 55.0 and 5.8 µm, respectively. Regarding the volumetric proportion, we reported the following values: epithelium volume 36.2 and 80.4%, lumen without spermatozoon 19.6 and 3.0%, lumen with spermatozoon 5.4 and 0.0%, interstitium 35.4 and 12.0%, blood vessels 3.5 and 4.6%, structures in cellular superficies 1.4 and 0%, lamina 1.4 and 3.2%, and artifacts 0.3 and 1.3%, respectively. The epididymis ducts had a circular form in transverse sections with spermatozoon only in November 2005 and December 2006. The Rheas epididymis morphology was found to be similar to ostriches, roosters, and Japanese quail. Here, we present data from stereological microscopy (tubular diameter, epithelium thickness, and lumen diameter), volumetric proportion (epithelium, lumen without spermatozoon, lumen with spermatozoon, interstitium, blood vessels, structures in cellular superficies; cilium, estereocilium, and lamina) in this species during the repose and sexual activity period (reproductive season).(AU)

O objetivo desta pesquisa foi estudar a histologia e descrever os achados microscópicos do epitélio epidídimo de ema em três períodos: novembro/2005 (n = 14), dezembro/2006 (n = 20) e maio/2007 (n = 20), para observação e comparação das diferenças que ocorreram nesses tempos distintos. Estudamos o epidídimo de 54 animais, criados em Santa Maria, RS. Durante o abate comercial, os epidídimos foram coletados e fixados em Bouin. A microscopia óptica mede a estrutura celular, tipos de células, túbulos e valores estereológicos, como os diâmetros do epitélio do epidídimo, lúmen, espessura, volume relativo da estrutura do tecido. Em dezembro/2006 e maio/2007, as médias do diâmetro tubular do epidídimo foram: 79,1 e 58,1µm, espessura do epitélio: 24,0 e 52,2µm, diâmetro do lúmen: 55,0 e 5,8µm, respectivamente. Em relação à proporção volumétrica, encontramos os seguintes valores: volume epitelial 36,2 e 80,4%, lúmen sem espermatozoide 19,6 e 3,0%, lúmen com espermatozoide 5,4 e 0,0%, intestínio 35,4 e 12,0%, vasos sanguíneos 3,5 e 4,6%, estruturas celulares superfícies 1,4 e 0%, lâmina 1,4 e 3,2%, artefatos 0,3 e 1,3%, respectivamente. Os ductos do epidídimo apresentaram forma circular em cortes transversais com espermatozoide apenas em novembro/2005 e dezembro/2006. A morfologia do epidídimo de Rhea foi semelhante ao avestruz, galo e codornas japonesas. Apresentamos dados de microscopia estereológica (diâmetro tubular, espessura do epitélio, diâmetro do lúmen), proporção volumétrica, em porcentagem (epitélio, lúmen sem espermatozóide, lúmen com espermatozoide, intestório, vasos sanguíneos, estruturas em superfícies celulares - cílio - estereocílio, lâmina e artefatos) nesta espécie nos períodos de repouso e atividade sexual (estação reprodutiva).(AU)

Morfologia e morfometria do encéfalo de avestruz (Struthio camelus) / Morphology and morphometry of the ostrich encephalus (Struthio camelus)

O avestruz africano é uma ave ratita da família Struthionidae. As características anatômicas do encéfalo do avestruz foram investigadas neste artigo. Vinte encéfalos de avestruzes adultos (dez machos e dez fêmeas), fixados em formaldeído a 10%, foram analisados neste estudo. Foram obtidas as medidas do encéfalo: comprimento e largura total do encéfalo, comprimento, largura e altura dos hemisférios cerebrais direito e esquerdo, comprimento e largura do cerebelo, comprimento e largura dos lobos ópticos direito e esquerdo. Também foram aferidos o comprimento e a largura do crânio. Os resultados para cada variável foram expressos em média e em desvio padrão. Através do teste t de Student não-pareado as variáveis foram comparadas entre os sexos e entre os antímeros. Considerou-se p<0,05 como significante. O comprimento e largura do encéfalo dos machos foram de 5,57 cm ± 0,23 cm e de 4,19 cm ± 0,16 cm, respectivamente. Nas fêmeas o comprimento e largura do encéfalo foram de 5,56 cm ± 0,23 cm e de 4,18 cm ± 0,16 cm respectivamente. O comprimento do hemisfério cerebral direito foi de 3,41 cm ± 0,13 cm nos machos e de 3,39 cm ± 0,12 cm nas fêmeas, já no esquerdo foi de 3,35 cm ± 0,14 cm nos machos e de 3,34 cm ± 0,05 cm nas fêmeas. A largura do hemisfério cerebral direito foi de 2,27 cm ± 0,17 cm nos machos e de 2,25 ± 0,14 cm nas fêmeas, e do esquerdo foi de 2,25 cm ± 0,11 cm nos machos e de 2,27 cm ± 0,11 cm nas fêmeas. A altura do hemisfério cerebral direito foi de 2,08 cm ± 0,23 cm nos machos e de 2,07 cm ± 0,21 cm nas fêmeas e do hemisfério cerebral esquerdo foi de 2,05 cm ± 0,22 cm nos machos e de 2,05 cm ± 0,22 nas fêmeas. O comprimento do cerebelo foi de 2,45 cm ± 0,32 cm nos machos e de 2,44 cm ± 0,32 cm nas fêmeas, e sua largura foi de 1,59 cm ± 0,16 cm nos machos e de 1,58 cm ± 0,15 cm nas fêmeas. O comprimento do lobo óptico direito foi de 1,30 cm ± 0,06 nos machos e de 1,30 cm ± 0,06 cm nas fêmeas, já o comprimento do lobo óptico esquerdo foi de 1,24 cm ± 0,07 cm nos machos e de 1,23 cm ± 0,67 cm nas fêmeas. A largura do lobo óptico direito foi de 1,08 cm ± 0,07 cm nos machos e de 1,08 cm ± 0,07 cm nas fêmeas e largura do lobo óptico esquerdo foi de 1,08 cm ± 0,07 cm nos machos e de 1,08 cm ± 0,07 cm nas fêmeas. A superfície do cérebro do avestruz é lisa sem qualquer giro ou sulco. A média do comprimento dos crânios dos machos é maior que a das fêmeas. Em relação aos 20 encéfalos analisados o lobo óptico direito é maior que o esquerdo. Houve correlação linear positiva entre a largura do crânio com a largura do cerebelo.

The african ostrich is a ratite bird in the family Struthionidae. The anatomical features of the African ostrich brain were investigated in this study. Twenty adult ostrich heads (10 males and 10 females), fixed in 10% formaldehyde, were analyzed in this study. The measures of the brain were obtained: length and total width of the brain, length, width and height of the right and left cerebral hemispheres, length and width of the cerebellum, length and width of the right and left optical lobes. The length and width of the skull were also measured. Results for each variable were expressed as mean and standard deviation. Through unpaired Student's ttest the variables were compared between sexes and between antimers. Statistical significance was considered as p<0,05. The length and width of the male encephalus was 5.57 ± 0.23 cm and 4.19 ± 0.16, respectively. In females, the length and width of the encephalus were 5.56 ± 0.23 and 4.18 ± 0.16 respectively. The length of the right cerebral hemisphere was 3.41 ± 0.13 in males and 3.39 ± 0.12 in females, while in the left it was 3.35 ± 0.14in males and 3.34 ± 0.05 in females. The width of the right cerebral hemisphere was 2.27 ± 0.17 in males and 2.25 ± 0.14 in females, and the left was 2.25 ± 0.11 in males and 2.27 ± 0.11 in females. The height of the right cerebral hemisphere was 2.08 ± 0.23 in males and 2.07 ± 0.21 in females and the left cerebral hemisphere was 2.05 ± 0.22 in males and 2.05 ± 0.22 in females. The length of the cerebellum was 2.45 ± 0.32 in males and 2.44 ± 0.32 in females, and its width was 1.59 ± 0.16 in males and 1.58 ± 0.15 in females. The length of the right optic lobe was 1.30 ± 0.06 in males and 1.30 ± 0.06 in females, while the length of the left optic lobe was 1.24 ± 0.07 in males and 1.23 ± 0.67 in females. The width of the right optic lobe was 1.08 ± 0.07 in males and 1.08 ± 0.07 in females and the width of the left optic lobe was 1.08 ± 0.07 in males and 1.08 ± 0.07 in females. The surface of the ostrich brain is smooth without any gyrus or sulcus. The average length of the skulls of males is greater than that of females. In relation to the 20 encephalus analyzed, the right optic lobe is larger than the left. There was a positive linear correlation between the width of the skull and the width of the cerebellum.

Morfologia e morfometria do encéfalo de avestruz (Struthio camelus) / Morphology and morphometry of the ostrich encephalus (Struthio camelus)

O avestruz africano é uma ave ratita da família Struthionidae. As características anatômicas do encéfalo do avestruz foram investigadas neste artigo. Vinte encéfalos de avestruzes adultos (dez machos e dez fêmeas), fixados em formaldeído a 10%, foram analisados neste estudo. Foram obtidas as medidas do encéfalo: comprimento e largura total do encéfalo, comprimento, largura e altura dos hemisférios cerebrais direito e esquerdo, comprimento e largura do cerebelo, comprimento e largura dos lobos ópticos direito e esquerdo. Também foram aferidos o comprimento e a largura do crânio. Os resultados para cada variável foram expressos em média e em desvio padrão. Através do teste t de Student não-pareado as variáveis foram comparadas entre os sexos e entre os antímeros. Considerou-se p<0,05 como significante. O comprimento e largura do encéfalo dos machos foram de 5,57 cm ± 0,23 cm e de 4,19 cm ± 0,16 cm, respectivamente. Nas fêmeas o comprimento e largura do encéfalo foram de 5,56 cm ± 0,23 cm e de 4,18 cm ± 0,16 cm respectivamente. O comprimento do hemisfério cerebral direito foi de 3,41 cm ± 0,13 cm nos machos e de 3,39 cm ± 0,12 cm nas fêmeas, já no esquerdo foi de 3,35 cm ± 0,14 cm nos machos e de 3,34 cm ± 0,05 cm nas fêmeas. A largura do hemisfério cerebral direito foi de 2,27 cm ± 0,17 cm nos machos e de 2,25 ± 0,14 cm nas fêmeas, e do esquerdo foi de 2,25 cm ± 0,11 cm nos machos e de 2,27 cm ± 0,11 cm nas fêmeas. A altura do hemisfério cerebral direito foi de 2,08 cm ± 0,23 cm nos machos e de 2,07 cm ± 0,21 cm nas fêmeas e do hemisfério cerebral esquerdo foi de 2,05 cm ± 0,22 cm nos machos e de 2,05 cm ± 0,22 nas fêmeas. O comprimento do cerebelo foi de 2,45 cm ± 0,32 cm nos machos e de 2,44 cm ± 0,32 cm nas fêmeas, e sua largura foi de 1,59 cm ± 0,16 cm nos machos e de 1,58 cm ± 0,15 cm nas fêmeas. O comprimento do lobo óptico direito foi de 1,30 cm ± 0,06 nos machos e de 1,30 cm ± 0,06 cm nas fêmeas, já o comprimento do lobo óptico esquerdo foi de 1,24 cm ± 0,07 cm nos machos e de 1,23 cm ± 0,67 cm nas fêmeas. A largura do lobo óptico direito foi de 1,08 cm ± 0,07 cm nos machos e de 1,08 cm ± 0,07 cm nas fêmeas e largura do lobo óptico esquerdo foi de 1,08 cm ± 0,07 cm nos machos e de 1,08 cm ± 0,07 cm nas fêmeas. A superfície do cérebro do avestruz é lisa sem qualquer giro ou sulco. A média do comprimento dos crânios dos machos é maior que a das fêmeas. Em relação aos 20 encéfalos analisados o lobo óptico direito é maior que o esquerdo. Houve correlação linear positiva entre a largura do crânio com a largura do cerebelo.(AU)

The african ostrich is a ratite bird in the family Struthionidae. The anatomical features of the African ostrich brain were investigated in this study. Twenty adult ostrich heads (10 males and 10 females), fixed in 10% formaldehyde, were analyzed in this study. The measures of the brain were obtained: length and total width of the brain, length, width and height of the right and left cerebral hemispheres, length and width of the cerebellum, length and width of the right and left optical lobes. The length and width of the skull were also measured. Results for each variable were expressed as mean and standard deviation. Through unpaired Student's ttest the variables were compared between sexes and between antimers. Statistical significance was considered as p<0,05. The length and width of the male encephalus was 5.57 ± 0.23 cm and 4.19 ± 0.16, respectively. In females, the length and width of the encephalus were 5.56 ± 0.23 and 4.18 ± 0.16 respectively. The length of the right cerebral hemisphere was 3.41 ± 0.13 in males and 3.39 ± 0.12 in females, while in the left it was 3.35 ± 0.14in males and 3.34 ± 0.05 in females. The width of the right cerebral hemisphere was 2.27 ± 0.17 in males and 2.25 ± 0.14 in females, and the left was 2.25 ± 0.11 in males and 2.27 ± 0.11 in females. The height of the right cerebral hemisphere was 2.08 ± 0.23 in males and 2.07 ± 0.21 in females and the left cerebral hemisphere was 2.05 ± 0.22 in males and 2.05 ± 0.22 in females. The length of the cerebellum was 2.45 ± 0.32 in males and 2.44 ± 0.32 in females, and its width was 1.59 ± 0.16 in males and 1.58 ± 0.15 in females. The length of the right optic lobe was 1.30 ± 0.06 in males and 1.30 ± 0.06 in females, while the length of the left optic lobe was 1.24 ± 0.07 in males and 1.23 ± 0.67 in females. The width of the right optic lobe was 1.08 ± 0.07 in males and 1.08 ± 0.07 in females and the width of the left optic lobe was 1.08 ± 0.07 in males and 1.08 ± 0.07 in females. The surface of the ostrich brain is smooth without any gyrus or sulcus. The average length of the skulls of males is greater than that of females. In relation to the 20 encephalus analyzed, the right optic lobe is larger than the left. There was a positive linear correlation between the width of the skull and the width of the cerebellum.(AU)

Observational study on the breeding performance of emus (Dromaius novaehollandiae) in Brazil / [Estudo observacional sobre o desempenho reprodutivo de emus (Dromaius novaehollandiae) no Brasil]

The objective of this study was to describe emus' breeding performance in Brazil at different ages, grouped in couples or colonies. The duration of the breeding season and the production of eggs per female housed were recorded, and the productivity and breeding variables were associated with the variation of the photoperiod. The total production of the flock was 180 eggs, and the breeding season lasted 167 days (April-September), a period with an average of 11 h and 11min of daylight. The breeding season lasted 113, 133 and 82 days, the numbers of eggs produced per female were 7.29, 25.67 and 17.3, and productivity values were 31.6, 38.6, and 45.4% in the groups of birds with ages of two, four and seven years, respectively. The breeding season in 2016 occurred between April and August in Brazil. Older birds tended to start breeding later. The production rate observations indicated that earlier peak production was associated with lower egg production potential. Finally, there was a tendency for better breeding performance of birds housed in couples than in groups with more birds.(AU)

O objetivo deste estudo foi descrever o desempenho reprodutivo de emus de diferentes idades, agrupados em casais ou em colônias, no Brasil. A duração da estação reprodutiva e a produção de ovos por fêmea alojada foram registradas, a produtividade e as variáveis reprodutivas foram associadas às variações no fotoperíodo. A produção total do lote de aves foi de 180 ovos, e a estação reprodutiva durou 167 dias (abril-setembro), período que apresentou fotoperíodo médio de 11 horas e 11 minutos. Nos grupos de aves de dois, quatro e sete anos de idade, a estação reprodutiva durou 113,, 133 e 82 dias, o número de ovos por fêmea foi de 7,29, 25,67 e 17,3 e a produtividade foi de 31,6, 38,6 e 45,4%, respectivamente. A estação reprodutiva em 2016 ocorreu entre abril e agosto no Brasil. Aves mais velhas tenderam a iniciar o período reprodutivo mais tarde. As observações na taxa de produção podem indicar que quanto mais precoce o pico produtivo, menor o potencial de produção de ovos em emus. Houve a tendência ao melhor desempenho reprodutivo das aves alojadas em casais em comparação com as alojadas em colônias.(AU)

Anatomia macroscópica e vascularização do encéfalo de emas (Rhea americana americana) / Macroscopic anatomy and brain vascularization in the greater rhea (Rhea americana americana)

Background: The Rhea americana americana is a wild bird belonging to the group of Ratites, and is important from the scientific point of view given their adaptability to captivity. Considering that information about its morphology is important for the viability of domesticating the species, the aim of this study was to macroscopically identify the brain regions, as well as the cerebral arteries and the cerebral arterial circuit in order to establish the cerebral vascular pattern and systematization.Materials, Methods & Results: Twenty one brains from young and adult Greater Rheas of both sexes were used from animals that had died due to natural causes and were then kept in a freezer. The specimens were thawed and incised in the cervical region to allow exposure of the left common carotid artery, which was cannulated. The vascular system was rinsed with 0.9% saline solution, then perfused with latex Neoprene 650 stained with red pigment. The animals were subsequently fixed in 3.7% aqueous formaldehyde solution for 72 h, and then they were dissected by removing the bones from the skull cap. The brains were analyzed, and the structures were identified, photographed, schematized and denominated. Morphometric measurements were performed on the basilar and cerebellar ventral caudal arteries, recording the values of length and width in millimeters with the aid of a digital caliper. The brain was divided into: telencephalon, diencephalon, brainstem and cerebellum; while externally, the observed structures are: olfactory bulbs, optical lobes, optic nerves, optic chiasm, pituitary and pineal glands. Vascularization was performed by the following arteries: ventral spinal artery, basilar artery, ventricular cerebellar arteries, medium ventricular cerebellar arteries, caudal branches of the carotid arteries of the brain, ventral mesencephalic artery, cerebral caudal arteries, rostral branches of the carotid arteries of the brain, middle cerebral arteries,[...]

Anatomia macroscópica e vascularização do encéfalo de emas (Rhea americana americana) / Macroscopic anatomy and brain vascularization in the greater rhea (Rhea americana americana)

Background: The Rhea americana americana is a wild bird belonging to the group of Ratites, and is important from the scientific point of view given their adaptability to captivity. Considering that information about its morphology is important for the viability of domesticating the species, the aim of this study was to macroscopically identify the brain regions, as well as the cerebral arteries and the cerebral arterial circuit in order to establish the cerebral vascular pattern and systematization.Materials, Methods & Results: Twenty one brains from young and adult Greater Rheas of both sexes were used from animals that had died due to natural causes and were then kept in a freezer. The specimens were thawed and incised in the cervical region to allow exposure of the left common carotid artery, which was cannulated. The vascular system was rinsed with 0.9% saline solution, then perfused with latex Neoprene 650 stained with red pigment. The animals were subsequently fixed in 3.7% aqueous formaldehyde solution for 72 h, and then they were dissected by removing the bones from the skull cap. The brains were analyzed, and the structures were identified, photographed, schematized and denominated. Morphometric measurements were performed on the basilar and cerebellar ventral caudal arteries, recording the values of length and width in millimeters with the aid of a digital caliper. The brain was divided into: telencephalon, diencephalon, brainstem and cerebellum; while externally, the observed structures are: olfactory bulbs, optical lobes, optic nerves, optic chiasm, pituitary and pineal glands. Vascularization was performed by the following arteries: ventral spinal artery, basilar artery, ventricular cerebellar arteries, medium ventricular cerebellar arteries, caudal branches of the carotid arteries of the brain, ventral mesencephalic artery, cerebral caudal arteries, rostral branches of the carotid arteries of the brain, middle cerebral arteries,[...](AU)

Hematological and morphometric differences of blood cells from rheas, Rhea americana (Struthioniformes: Rheidae) on two conservation farms / Diferenças hematológicas e morfométricas de células sanguineas de emas, Rhea americana (Struthioniformes: Rheidae) em dois criatórios conservacionistas

Today, blood tests are an indispensable tool in avian medicine. This study aimed to describe and compare hematological and morphometric data of erythrocytes, leukocytes and thrombocytes of rhea, Rhea americana. To do so, 58 rheas of both sexes on two farms, one in Cachoeiro do Itapemirim, Espيrito Santo (ES), and the other in Sمo Carlos, Sمo Paulo (SP), were selected. Blood samples were taken and their RBC counts, PCV and Hb levels were determined and used in hematimetric index calculations. The total and differentiated leukocyte counts and the TPP and fibrinogen serum levels were also obtained. The results from the two farms analyzed were compared by means of the t test. There were differences in the parameters of the erythrocyte series between the two flocks because of a process of hypochromic macrocytic anemia observed among the rheas in ES. The values for the erythrocyte series and indexes were: RBC, 2.81 ± 0.15 x106/l; PCV, 44.20 ± 2.86%; Hb, 12.12 ± 0.74 g/dL; MCV, 15.75 ± 0.89 fL; MCH, 43.18 ± 1.82 pg; and MCHC, 27.44 ± 0.80 g/dL. The values for the leukocyte series were: WBC, 12,072 ± 4116 /L; heterophils, 64.10 ± 9.90%; eosinophils, 2.05 ± 2.06%; monocytes, 6.40 ± 2.99%; lymphocytes, 26.93 ± 9.62%; and basophils, 0.52 ± 1.27%. These can all be suggested as references for rheas reared in Brazil. Statistical differences were observed in erythrocyte length measurements, which were higher in the rheas in SP than in those in ES. Regarding width, the heterophils, lymphocytes and monocytes of the birds in ES were statistically greater than those of the birds in SP. It was concluded that rheas may exhibit morphometric alterations to blood cells and differences in blood elements according to the type of management to which they are subjected.(AU)

Atualmente exames de sangue são ferramentas indispensáveis na medicina aviária. Este estudo teve como objetivo descrever e comparar valores hematológicos e morfométricos de eritrócitos, leucócitos e trombócitos de emas, Rhea americana. Para isso, foram selecionadas 58 emas de ambos os sexos de dois criatórios, um em Cachoeiro do Itapemirim, Espírito Santo (ES) e o outro em São Carlos, São Paulo (SP). Amostras de sangue foram coletadas e realizadas a hematimetria, Hto e dosagem de Hb que foram utilizados nos cálculos dos índices hematimétricos. Também foram efetuadas as contagens total e diferencial de leucócitos, além da dosagem de proteínas total e do fibrinogênio séricos. Os resultados referentes às duas propriedades analisadas foram comparados pelo Teste t. Ocorreram diferenças nos parâmetros da série vermelha entre as aves dos dois criatórios devido a um processo de anemia macrocítica hipocrômica observado nas emas do ES. Os valores para a série vermelha e índices hematimétricos como: hematimetria, 2,81±0,15 x106/μl; Hto, 44,20±2,86%; Hb, 12,12±0,74 g/dL; VCM, 15,75±0,89 fL; HCM, 43,18±1,82 pg; CHCM, 27,44±0,80, além dos valores da série branca como: leucometria total, 12.072±4.116/μL, heterofilo, 64,10±9,90%, eosinófilo, 2,05±2,06%, monócito, 6,40±2,99%, linfócito, 26,93±9,62%, basófilo, 0,52±1,27% foram sugeridos como os de referência para as emas criadas no Brasil. Diferenças estatísticas foram observadas no comprimento de eritrócitos, o qual estava maior nas emas de SP do que aquelas do ES. Em relação à largura, os heterófilos, linfócitos e monócitos das aves do ES foram estatisticamente maior do que aquelas das aves de SP. Concluiu-se que emas podem apresentar alterações morfométricas de células sanguíneas e diferenças nos elementos do sangue de acordo com o tipo de manejo a que são submetidos.(AU)

Morfologia das gônadas de ema (Rhea americana) em desenvolvimento: diferenciação sexual / Morphology of the gonads of rhea (Rhea americana) in development: sexual differentiation

The aim of this study was to evaluate the morphogenesis of rheas reproduction equipment featuringgonadal differentiation phase. We used eight fetuses, obtained from eggs, the Study Group Preservation of WildAnimals UFPI. The animals were fixed in formalin 5% 70% alcohol, dissected and analyzed bystereomicroscopy. Fragments of the urogenital organs were dehydrated in alcohol, diaphanized in xyleneemblocados and subjected to 5μm sections stained with hematoxylin-eosin. It was found in fetus 14 days theurogenital structures as two elongated bodies, dorsally in the coelomic cavity, lateral to the midline, andflattened dorsoventrally, the urogenital ridge; 18 days were divided medially, forming new ventral massprimitive gonads and a dorsal primitive kidneys; 21 days, were completely separated, the lobate kidneys andgonads with smooth surfaces and fetus of 30 days was observed the evolution of the development of the gonads.The rhea sexual differentiation phase occurs on the 14th day of fetal development.(AU)

Morfologia das gônadas de ema (Rhea americana) em desenvolvimento: diferenciação sexual / Morphology of the gonads of rhea (Rhea americana) in development: sexual differentiation

The aim of this study was to evaluate the morphogenesis of rheas reproduction equipment featuringgonadal differentiation phase. We used eight fetuses, obtained from eggs, the Study Group Preservation of WildAnimals UFPI. The animals were fixed in formalin 5% 70% alcohol, dissected and analyzed bystereomicroscopy. Fragments of the urogenital organs were dehydrated in alcohol, diaphanized in xyleneemblocados and subjected to 5μm sections stained with hematoxylin-eosin. It was found in fetus 14 days theurogenital structures as two elongated bodies, dorsally in the coelomic cavity, lateral to the midline, andflattened dorsoventrally, the urogenital ridge; 18 days were divided medially, forming new ventral massprimitive gonads and a dorsal primitive kidneys; 21 days, were completely separated, the lobate kidneys andgonads with smooth surfaces and fetus of 30 days was observed the evolution of the development of the gonads.The rhea sexual differentiation phase occurs on the 14th day of fetal development.

Aspects of spermatogenesis and microscopic testicular morphology in Greater Rhea, Rhea americana (Linnaeus, 1758) / Aspectos da espermatogênese e morfologia microscópica testicular em Emas, Rhea americana (Linnaeus, 1758)

The purpose of this study was to study the microscopic morphology of the testicular parenchyma of Rhea americana birds. Fifty-four 2.5±0.5 year-old male adults bred in captivity. were used. During commercial slaughter, samples of testis were collected in November/2005, December/2006 and May/2007, in order to compare possible differences. The samples underwent optical microscopy analysis and measurements of seminiferous tubule (ST) total diameters, lumen, epithelium thickness and the relative volume of parenchyma. The ST had circular form in transverse cross sections. November/2005 and December/2006 samples had many types of germinative cells and spermatozoa in lumen, but in May/2007 the samples of epithelium were poor regarding meiotic and mitotic pictures, and it was difficult to find any spermatozoon; in many tubules the lumen was inexistent or diminished. In December/2006 and May/2007 the averages were: tubule diameter 110.3 and 5.3mµ, lumen 52.4 and 4.5mµ, epithelium thickness 57.8 and 0.7mµ respectively. The volumetric proportions were: seminiferous epithelium 75.6 and 75.9, cysts in epithelium 2.1 and 1.0, ST 93.3 and 84.0, interstitium 6.2 and 15.6 respectively. The sperm reserves were: 19.7±2 and 0±0 x109 sperm cells in December 2006 and May 2007 respectively. Microscopic measures of seminiferous tubules, spermatic cells and diameter of the nuclei were presented. These data confirm reproductive seasonality, with breeding season in spring-summer with sperm production. A great variation n parenchyma, when compared breeding was noticeable.(AU)

O objetivo do estudo foi estudar a morfologia microscópica do parênquima testicular de emas (Rhea americana). Foram utilizados 54 machos adultos criados em cativeiro de 2,5±0,5 anos de idade. Durante o abate comercial foram coletadas amostras de testículos em novembro/2005, dezembro/2006 e maio/2007, para efeitos de comparação. As amostras foram processadas e para microscopia ótica de rotina para análise. Foram medidas diâmetro total de túbulos seminíferos (ST), lúmen, espessura do epitélio e a proporção volumétrica dos componentes do parênquima. O ST apresentou forma circular nas seções transversais. Em novembro/2005 e dezembro/2006, se observaram os tipos de células germinativas e espermatozoides no lúmen. Em maio/2007, as amostras de epitélio se observaram escassas meioses e imagens de mitose e era difícil de ver qualquer espermatozoide, em muitos dos túbulos o lúmen era inexistentes ou diminuído de tamanho. Em dezembro/2006 e maio/2007, as médias das características estudadas foram: diâmetro dos túbulos 110,3 e 5,3 mµ, lúmen 52,4 e 4,5mµ, espessura do epitélio 57,8 e 0,7mµ, respectivamente. As proporções volumétricas foram: epitélio seminífero 75,6 e 75,9, cistos no epitélio 2,1 e 1,0, túbulos seminíferos 93,3 e 84,0, interstício 6,2 e 15,6, respectivamente. Foram apresentadas medidas microscópicas de túbulos seminíferos, diâmetro dos núcleos das espermátides. Estes dados confirmam a sazonalidade reprodutiva, com época de reprodução na primavera - verão, com a produção de esperma. Foi perceptível uma grande variação nas medidas do parênquima testicular, quando se comparou a estação reprodutiva.(AU)

Aspects of spermatogenesis and microscopic testicular morphology in Greater Rhea, Rhea americana (Linnaeus, 1758) / Aspectos da espermatogênese e morfologia microscópica testicular em Emas, Rhea americana (Linnaeus, 1758)

The purpose of this study was to study the microscopic morphology of the testicular parenchyma of Rhea americana birds. Fifty-four 2.5±0.5 year-old male adults bred in captivity. were used. During commercial slaughter, samples of testis were collected in November/2005, December/2006 and May/2007, in order to compare possible differences. The samples underwent optical microscopy analysis and measurements of seminiferous tubule (ST) total diameters, lumen, epithelium thickness and the relative volume of parenchyma. The ST had circular form in transverse cross sections. November/2005 and December/2006 samples had many types of germinative cells and spermatozoa in lumen, but in May/2007 the samples of epithelium were poor regarding meiotic and mitotic pictures, and it was difficult to find any spermatozoon; in many tubules the lumen was inexistent or diminished. In December/2006 and May/2007 the averages were: tubule diameter 110.3 and 5.3m, lumen 52.4 and 4.5m, epithelium thickness 57.8 and 0.7m respectively. The volumetric proportions were: seminiferous epithelium 75.6 and 75.9, cysts in epithelium 2.1 and 1.0, ST 93.3 and 84.0, interstitium 6.2 and 15.6 respectively. The sperm reserves were: 19.7±2 and 0±0 x109 sperm cells in December 2006 and May 2007 respectively. Microscopic measures of seminiferous tubules, spermatic cells and diameter of the nuclei were presented. These data confirm reproductive seasonality, with breeding season in spring-summer with sperm production. A great variation n parenchyma, when compared breeding was noticeable.(AU)

O objetivo do estudo foi estudar a morfologia microscópica do parênquima testicular de emas (Rhea americana). Foram utilizados 54 machos adultos criados em cativeiro de 2,5±0,5 anos de idade. Durante o abate comercial foram coletadas amostras de testículos em novembro/2005, dezembro/2006 e maio/2007, para efeitos de comparação. As amostras foram processadas e para microscopia ótica de rotina para análise. Foram medidas diâmetro total de túbulos seminíferos (ST), lúmen, espessura do epitélio e a proporção volumétrica dos componentes do parênquima. O ST apresentou forma circular nas seções transversais. Em novembro/2005 e dezembro/2006, se observaram os tipos de células germinativas e espermatozoides no lúmen. Em maio/2007, as amostras de epitélio se observaram escassas meioses e imagens de mitose e era difícil de ver qualquer espermatozoide, em muitos dos túbulos o lúmen era inexistentes ou diminuído de tamanho. Em dezembro/2006 e maio/2007, as médias das características estudadas foram: diâmetro dos túbulos 110,3 e 5,3 m, lúmen 52,4 e 4,5m, espessura do epitélio 57,8 e 0,7m, respectivamente. As proporções volumétricas foram: epitélio seminífero 75,6 e 75,9, cistos no epitélio 2,1 e 1,0, túbulos seminíferos 93,3 e 84,0, interstício 6,2 e 15,6, respectivamente. Foram apresentadas medidas microscópicas de túbulos seminíferos, diâmetro dos núcleos das espermátides. Estes dados confirmam a sazonalidade reprodutiva, com época de reprodução na primavera - verão, com a produção de esperma. Foi perceptível uma grande variação nas medidas do parênquima testicular, quando se comparou a estação reprodutiva.(AU)

Comparative morphology of the species of Libyostrongylus and Codiostomum, parasites from ostriches, Struthio camelus, with a identification key to the species / Morfologia Comparativa das espécies de Libyostrongylus e Codiostomum, parasitas de avestruzes, Struthio camelus, com uma chave para identificação das espécies

One of the most common problems in breeding of ostriches in captivity is the control of parasitic diseases. This work presents keys for the identification of adult nematodes and infective larvae by morphologic and morphometric characteristics. These keys will allow the scientific community to identify the species that infect the ostriches either based on the characteristics of the posterior end of the infective larvae found through a simple fecal exam or by observing the morphology and morphometry of adult worms recovered during necropsies. These keys will facilitate ecological and systematic studies, as well as increase the understanding of the epidemiology of these parasitosis in ostriches.

Um dos problemas mais comuns na criação de avestruzes em cativeiro é o controle das doenças parasitárias. Este trabalho apresenta chaves para a identificação de Nematoda adultos e larvas infectantes através de caracteres morfológicos e morfométricos. Essas chaves de identificação permitirão à comunidade científica o diagnóstico das espécies que infectam as avestruzes com base nas características da extremidade posterior das larvas infectantes encontradas por meio de simples exames fecais ou pela observação da morfologia e morfometria dos espécimes adultos recuperados durante necropsia. Dessa forma, as chaves de identificação facilitarão os estudos ecológicos e sistemáticos, bem como a melhor compreensão da epidemiologia dessas infecções em avestruzes.

Occurrence of antibodies to Toxoplasma gondii in rheas (Rhea americana) and ostriches (Struthio camelus) from farms of different Brazilian regions / Ocorrência de anticorpos Toxoplasma gondii em emas (Rhea americana) e avestruzes (Struthio camelus) de propriedades de diferentes regiões brasileiras

This study aimed to verify the occurrence of antibodies against Toxoplasma gondii in rheas (Rhea americana) and ostriches (Struthio camelus) commercially breeding in Brazil. Blood samples from 20 rheas and 46 ostriches (young and adults) were serologically tested using a technique known as modified agglutination test (MAT) at an initial titration of 1:16 for ostriches and 1:25 for rheas. Antibodies against T. gondii were found in 50% (10/20) of the rheas, with titers ranging from 1:25 to 1:6,400. The incidence of antibodies against T. gondii in ostriches was 17.4% (8/46) with titers ranging from 1:16 to 1:256. Birds showing titers higher than 1:200 for T. gondii were mainly the young ones. Therefore, rheas and ostriches may be parasitized by T. gondii, showing high levels of antibodies against this parasite.

Este estudo teve como objetivo verificar a presença de anticorpos contra o Toxoplasma gondii em emas (Rhea americana) e avestruzes (Struthio camelus) criados comercialmente no Brasil. Amostras de sangue de 20 emas e 46 avestruzes (jovens e adultos) foram testadas sorologicamente pela técnica conhecida como teste de aglutinação modificada (MAT) em titulações iniciais de 1:16 para avestruzes e 1:25 para emas. Anticorpos contra T. gondii foram encontrados em 50% (10/20) das emas com títulos variando de 1:25 a 1:6.400. A incidência de anticorpos contra T. gondii em avestruzes foi de 17,4% (8/46) com os títulos oscilando entre 1:16 e 1:256. Aves jovens foram aquelas que apresentaram títulos superiores a 1:200 para T. gondii. Portanto, emas e avestruzes podem ser parasitados por T. gondii, apresentando elevados níveis de anticorpos contra o parasita.

Occurrence of antibodies to Toxoplasma gondii in rheas (Rhea americana) and ostriches (Struthio camelus) from farms of different Brazilian regions

This study aimed to verify the occurrence of antibodies againstToxoplasma gondii in rheas (Rhea americana) and ostriches (Struthio camelus) commercially breeding in Brazil. Blood samples from 20 rheas and 46 ostriches (young and adults) were serologically tested using a technique known as modified agglutination test (MAT) at an initial titration of 1:16 for ostriches and 1:25 for rheas. Antibodies against T. gondii were found in 50% (10/20) of the rheas, with titers ranging from 1:25 to 1:6,400. The incidence of antibodies against T. gondii in ostriches was 17.4% (8/46) with titers ranging from 1:16 to 1:256. Birds showing titers higher than 1:200 forT. gondii were mainly the young ones. Therefore, rheas and ostriches may be parasitized by T. gondii, showing high levels of antibodies against this parasite.

Este estudo teve como objetivo verificar a presença de anticorpos contra o Toxoplasma gondii em emas (Rhea americana) e avestruzes (Struthio camelus) criados comercialmente no Brasil. Amostras de sangue de 20 emas e 46 avestruzes (jovens e adultos) foram testadas sorologicamente pela técnica conhecida como teste de aglutinação modificada (MAT) em titulações iniciais de 1:16 para avestruzes e 1:25 para emas. Anticorpos contra T. gondii foram encontrados em 50% (10/20) das emas com títulos variando de 1:25 a 1:6.400. A incidência de anticorpos contra T. gondii em avestruzes foi de 17,4% (8/46) com os títulos oscilando entre 1:16 e 1:256. Aves jovens foram aquelas que apresentaram títulos superiores a 1:200 para T. gondii. Portanto, emas e avestruzes podem ser parasitados por T. gondii, apresentando elevados níveis de anticorpos contra o parasita.

Parasitological and molecular detection of cryptosporidium parvum in rheas (Rhea americana)

Background: The rhea (Rhea americana) is a Brazilian wild bird that produce meat, leather and feathers of excellent quality. Rhea production has been increasing every day in Brazil due to many favorable conditions for breeding and there are also large native populations in various regions of the country. The incidence of parasites is a limiting factor when raising many animals, and rheas are not an exception. The occurrence of Cryptosporidium spp in captive rheas in a Brazilian zoo and Spain was described. However, little is known about cryptosporidiosis in rhea, which makes the need for further studies. Thus, this study aimed to detect Cryptosporidium parvum in rheas from the South of Brazil. Case: This study evaluated two properties located in Southern Brazil. Property A was located in Rio Rufi no, Santa Catarina State, Brazil and it had 40 rheas for commercial purposes. Property B was located in Santa Maria, a small town in Rio Grande do Sul State, Brazil and it had 10 rheas. Fresh fecal samples were collected and kept refrigerated from adult birds (n = 4) and chicks (n = 2) from property A, and chicks (n = 3) of three months of age from property B. Samples were analyzed by the method of direct examination, followed by centrifugal flotation with zinc sulfate. Only the centrifugal flotation technique allowed the observation of Cryptosporidium spp. oocysts in three adults and one chick. Fecal samples were stored in ethanol and analyzed by PCR for C. parvum, all being positive for this protozoan. Feces contaminated by C. parvum oocysts from one young rhea was used to inoculate two mice orally (BALB/c), previously confirmed protozoan free by faecal examination and PCR. Feces from inoculated mice were collected on days 1, 3 e 5 post-inoculation for analysis by the centrifugal flotation technique. After five days of inoculation all mice presented diarrhea and high numbers of oocysts of protozoan in their feces. Discussion: Cryptosporidiosis can evolve into severe diarrhea, followed by abdominal cramps, anorexia, vomiting, dehydration, nausea and fever in different animals. However the incubation period of this disease in rheas is unknown because this is only the third report of cryptosporidiosis in this wild bird. The Cryptosporidium spp. is an obligate parasite of vertebrates, and its colonization occurs at the periphery of the intestinal cells of the host and it may cause atrophy of these structures and enteritis, but these findings have not been described in rheas yet. The species of Cryptosporidium that are mainly reported in birds are Cryptosporidium meleagridis, Cryptosporidium baileyi and Cryptosporidium galli. In our study, the molecular analysis was performed in order to identify the protozoan, being detected the C. parvum, a zoonotic agent reported in several mammals. The rhea is wild birds with habits similar to ostriches. Already, in studies have reported the occurrence of Cryptosporidium in ostriches; however the species identified were different from that described in this study. Based on these results, we conclude that rhea may be parasitized by C. parvum, an important zoonotic parasite. Prevalence studies should be conducted in this area to estimate the role and impact of rhea as reservoirs and disseminators of this zoonotic parasite.

Parasitological and molecular detection of cryptosporidium parvum in rheas (Rhea americana) / Parasitological and molecular detection of cryptosporidium parvum in rheas (Rhea americana)

Background: The rhea (Rhea americana) is a Brazilian wild bird that produce meat, leather and feathers of excellent quality. Rhea production has been increasing every day in Brazil due to many favorable conditions for breeding and there are also large native populations in various regions of the country. The incidence of parasites is a limiting factor when raising many animals, and rheas are not an exception. The occurrence of Cryptosporidium spp in captive rheas in a Brazilian zoo and Spain was described. However, little is known about cryptosporidiosis in rhea, which makes the need for further studies. Thus, this study aimed to detect Cryptosporidium parvum in rheas from the South of Brazil.Case: This study evaluated two properties located in Southern Brazil. Property A was located in Rio Rufi no, Santa Catarina State, Brazil and it had 40 rheas for commercial purposes. Property B was located in Santa Maria, a small town in Rio Grande do Sul State, Brazil and it had 10 rheas. Fresh fecal samples were collected and kept refrigerated from adult birds (n = 4) and chicks (n = 2) from property A, and chicks (n = 3) of three months of age from property B. Samples were analyzed by the method of direct examination, followed by centrifugal flotation with zinc sulfate. Only the centrifugal fl otation technique allowed the observation of Cryptosporidium spp. oocysts in three adults and

Background: The rhea (Rhea americana) is a Brazilian wild bird that produce meat, leather and feathers of excellent quality. Rhea production has been increasing every day in Brazil due to many favorable conditions for breeding and there are also large native populations in various regions of the country. The incidence of parasites is a limiting factor when raising many animals, and rheas are not an exception. The occurrence of Cryptosporidium spp in captive rheas in a Brazilian zoo and Spain was described. However, little is known about cryptosporidiosis in rhea, which makes the need for further studies. Thus, this study aimed to detect Cryptosporidium parvum in rheas from the South of Brazil.Case: This study evaluated two properties located in Southern Brazil. Property A was located in Rio Rufi no, Santa Catarina State, Brazil and it had 40 rheas for commercial purposes. Property B was located in Santa Maria, a small town in Rio Grande do Sul State, Brazil and it had 10 rheas. Fresh fecal samples were collected and kept refrigerated from adult birds (n = 4) and chicks (n = 2) from property A, and chicks (n = 3) of three months of age from property B. Samples were analyzed by the method of direct examination, followed by centrifugal flotation with zinc sulfate. Only the centrifugal fl otation technique allowed the observation of Cryptosporidium spp. oocysts in three adults and

MORFOLOGIA DO ENCÉFALO DE EMAS (Rhea americana americana LINNAEUS, 1758)

Objetivou-se identificar macroscopicamente as regiões do encéfalo, bem como, as arterias cerebrais e o circuito arterioso cerebral, de modo a estabelecer um padrão vascular e sua sistematização, assim como, quantificar sua morfometria e estereologia. Para tanto, foram utilizados 21 encéfalos de emas, sendo 13 fêmeas e 2 machos (idades de 18 meses) e, 4 fêmeas e 2 machos (idades de 2 meses) para o estudo da vascularização e 22 encéfalos, sendo 2 fêmeas e 1 macho (1 mês), 1 fêmea e 2 machos (2 meses), 2 fêmeas e 1 macho (3, 4, 5 e 6 meses) e, 2 fêmeas e 2 machos (18 meses) para as análises de morfometria e estereologia. Os animais foram descongelados e incisados no plano sagital, na região cervical, de modo a expor a artéria carótida comum esquerda, a qual foi canulada e o sistema vascular lavado com solução salina a 0,9%, e logo após, perfundido com látex Neoprene 650, corado com pigmento vermelho, de forma a permitir uma melhor visualização das artérias. Já para o estudo morfométrico e estereológico, as carótidas foram canuladas e perfundidas com cerca de 5-10 ml de formaldeído a 3,7%, a fim de obter a fixação dos encéfalos. Posteriormente, as peças foram imersas em solução aquosa de formaldeído a 3,7%, durante 72 horas, e depois, iniciaram-se as dissecações e remoção dos ossos da calota craniana. Em seguida, os encéfalos foram analisados, sendo as estruturas, identificadas, mensuradas, fotografadas, esquematizadas e nominadas. Quanto à estereologia, os encéfalos foram incluídos em gelatina incolor, mantidos em refrigerador por 24 horas e, posteriormente, obteve-se cortes de 4 mm de espessura. Em seguida, foi removida a gelatina, realizada a coloração de Mainland e efetivada a quantificação do volume cerebral (V= T. a/p. pi). O encéfalo dividiu-se em: telencéfalo, diencéfalo, tronco cerebral e cerebelo e, externamente, observaram-se os bulbos olfatórios, lobos ópticos, nervos ópticos, quiasma óptico, hipófise e pineal. Já quanto ao estudo da vascularização, foram identificadas as artérias: espinhal ventral, basilar, cerebelares ventrais caudais, cerebelares ventrais médias, ramos caudais das artérias carótidas do cérebro, tectais mesencefálicas ventrais, cerebrais caudais, ramos rostrais das artérias carótidas do cérebro, cerebrais médias, cerebroetmoidais, anastomose intercerebral rostral, cerebrais rostrais, etmoidais, oftálmicas internas, inter-hemisférica, hipofisarias, tectais mesencefálicas dorsais, cerebelares dorsais, ramos hemisféricos occipitais, pineais e ramos hemisférios dorsais. O circuito arterioso cerebral apresentou-se de forma fechada, caudalmente e rostralmente em 100% dos espécimes. O encéfalo possuiu mensurações que chegaram até 50,267 mm de comprimento, 41,855 mm de largura, 29,320 mm de altura e 21,774 g de massa. Os lobos ópticos apresentaram valores de comprimento direito (12,647 mm), comprimento esquerdo (12,355 mm), largura direita (11,470 mm), largura esquerda (10,827 mm) e, altura direita (15,495 mm) e altura esquerda (15,122 mm). Já o cerebelo apresentou valores de até 21,462 mm (comprimento), 18,207 mm (largura) e 21,487 mm (altura). Quanto a estereologia do encéfalo, este variou de 33,663 cm³ e 71,971 cm³ (idades de 1-18 meses), respectivamente.

The objective of this study was to identify macroscopically the regions of the brain, as well as the cerebral arteries and the cerebral arterial circuit, in order to establish a vascular pattern and its systematization, as well as to quantify its morphometry and stereology. In order to do so, 21 greater rhea brains were used, 13 females and 2 males (ages 18 months) and 4 females and 2 males (ages 2 months) for the study of vascularization and 22 encephalus, 2 females and 1 male (1 month), 1 female and 2 male (2 months), 2 females and 1 male (3, 4, 5 and 6 months) and 2 females and 2 males (18 months) for morphometry and stereology analyzes. The animals were thawed and incised in the sagittal plane, in the cervical region, in order to expose the left common carotid artery, which was cannulated and the vascular system washed with 0.9% saline, and then perfused with Neoprene latex 650, stained with red pigment, in order to allow a better visualization of the arteries. For the morphometric and stereological study, the carotids were cannulated and perfused with about 5-10 ml of 3.7% formaldehyde in order to obtain the fixation of the brains. Subsequently, the pieces were immersed in aqueous solution of 3.7% formaldehyde for 72 hours, and then, dissections and removal of the bones from the skull cap were initiated. Then, the brains were analyzed, and the structures were identified, measured, photographed, schematized and nominated. As for stereology, the brains were included in colorless gelatin, held in refrigerator for 24 hours, and subsequently cut 4 mm thick. Gelatin was then removed, Mainland stained and quantification of brain volume was performed (V = T. a / p. pi). The brain was divided into: telencephalon, diencephalon, brain stem and cerebellum, and, externally, olfactory bulbs, optic lobes, optic nerves, optic chiasm, pituitary and pineal were observed. Ventral spinal, basilar, ventral cerebellar, mid-ventricular cerebellar, caudal branches of the carotid arteries of the brain, ventral mesencephalic arteries, caudal cerebral arteries, carpal arteries of the brain, middle cerebral arteries , cerebroethmoidal, rostral intercerebral anastomosis, cerebellar rostrais, ethmoidal, internal ophthalmic, interhemispheric, pituitary, dorsal mesencephalic, dorsal cerebellar, occipital, pineal and dorsal hemispherical branches. The cerebral arteriosclerotic circuit was closed, caudally and rostrally in 100% of the specimens. The brain had measurements that reached up to 50.267 mm in length, 41.855 mm in width, 29.320 mm in height and 21.774 g in mass. Optical lobes presented values of right length (12.647 mm), left length (12.355 mm), right width (11.470 mm), left width (10.827 mm) and right height (15.495 mm) and left height (15.112 mm). The cerebellum presented values of up to 21.462 mm (length), 18.207 mm (width) and 21.487 mm (height). As to the stereology of the encephalon, this varied from 33.663 cm³ and 71.971 cm³ (ages 1-18 months), respectively.

Nematoides gastrintestinais e Cryptosporidium sp. em avestruzes e fatores associados à infecção no Polo Regional do Paraguaçu, Estado da Bahia

The parasites are responsible for economic losses in the ostrich, by reducing the production and productivity. To determine the frequency and the factors associated with transmission of gastrointestinal nematodes and Cryptosporidium sp. in ostriches, 342 faecal samples were collected from 188 adults and 154 young ostriches raised in seven herds in the Regional Pole of Paraguaçu. Samples were evaluated for fecal eggs count, nematodes third-stage larvae and Cryptosporidium sp oocysts frequency. The data related to animals and their environments have been obtained by visiting the properties and interviews with producers. The association between parasite burden and the variables of management of the animals was analyzed by chi-square and "Odds ratio", with an interval of 95%. In all flocks were found infected birds. In 88.3% of stool samples were detected eggs of gastrointestinal nematodes, and 18.8% Cryptosporidium sp oocysts. Libyostrongylus dentatus (98%), L.douglassii (2%) and Codiostomum sp (2%) larvae were identified in faecal cultures. Environmental and management factors were associated with risk enteric parasites in ostrich farms. It was found that the ostriches kept in paddocks of irregular topography, in the absence of other animals or receiving water coming from wells or cisterns had lower chances of infection by nematodes. As for the Cryptosporidium genus, the creation in flat areas, with the presence of other animals in the paddocks and the supply of river water increases the chances (2.7 times) of infection.

Os parasitos são responsáveis por prejuízos econômicos na estrutiocultura, visto reduzirem a produção. Para determinar a frequência de nematoides gastrintestinais e de Cryptosporidium sp., em avestruzes, bem como os fatores associados a transmissão, foram colhidas amostras de fezes de 342 avestruzes, dos quais 188 eram adultos e 154 jovens, criados em sete plantéis no Polo Regional do Paraguaçu, para a contagem de ovos por grama de fezes, identificação de larvas de terceiro estádio de nematoides e de oocistos de Cryptosporidium sp. Os dados relacionados aos animais e ao ambiente foram obtidos por meio de visita às propriedades e entrevistas com os produtores. A associação entre a carga parasitária e às variáveis de manejo dos animais foi analisada pelo teste Qui-quadrado e por Razão de Odds, com intervalo de confiança de 95%. Em todos os plantéis, foram encontradas aves infectadas. Em 88,3% das amostras de fezes, foram detectados ovos de nematoides gastrointestinal, e, em 18,8%, oocistos de Cryptosporidium sp. Larvas de Libyostrongylus dentatus (98%), L.douglassii (2%) e Codiostomum sp. (2%) foram identificadas nas coproculturas. Fatores ambientais e de manejo estão associados com risco de enteroparasitos em criações de avestruz. As aves mantidas em piquetes de topografia acidentada, na ausência de outros animais ou que recebiam água procedente de poços ou cisternas, apresentavam menores chances de infecção por nematoides, enquanto para o gênero Cryptosporidium, as criações em áreas planas, com presença de outros animais nos piquetes e o fornecimento da água do rio aumentam as chances (2,7 vezes) de infecção.

Estimativa de correlações entre medidas morfométricas, peso do ovo e peso de filhotes de emas criados em cativeiro

Sixty-eight eggs of Rhea had been collected, in the period of 8 August to 13 September of 2003, in Quirinópolis, Goyas, Brazil. The objective was to evaluate the correlation between eggs weight (PO), morfometrics characteristics: eggs length (CO), eggs width (LO), eggs circumference (CIO), air chambers length (CCA), rinds thickness (EC); births weight (PN) and weights equivalence (EP). The eggs weight had significant correlation (P 001) with CO, LO e CIO; CO presented significant correlation (P 005) com LO e CIO; LO was correlated significantly (P 001) com CIO e (P 005) CCA; the CIO had significant correlation (P > 005) with CCA; CCA had significant correlation (P 001) with EC e PN; the EC presented significant correlation (P 001) with PN and the PN had significant correlation (P 001) with EP. The eggs weight do not constitute selection characteristic and the air chamber length (CCA) was the considered ideal variable for selection viable embryos to the fifteen days of incubation, when they are commercialized fertilized.

Foram coletados 68 ovos de Ema no período de 8 de agosto a 13 de setembro de 2003, em Quirinópolis, Goiás, Brasil. O objetivo foi avaliar a correlação entre peso do ovo (PO), características morfométricas: comprimento do ovo (CO), largura do ovo (LO), circunferência do ovo (CIO), comprimento da câmara de ar (CCA), espessura da casca (EC); peso ao nascimento (PN) e equivalência de peso (EP). O peso do ovo teve correlação significativa (P 0,01) com CO, LO e CIO; CO apresentou correlação significativa (P 0,05) com LO e CIO; LO foi correlacionada significativamente (P 0,01) com CIO e (P 0,05) CCA; a CIO teve correlação significativa (P > 0,05) com CCA; CCA teve correlação significativa (P 0,01) com EC; a EC apresentou correlação significativa (P 0,01) com PN e PN teve correlação significativa (P 0,01) com EP. Observou-se que o peso do ovo não constitui característica de seleção e o comprimento da câmara de ar (CCA) foi a variável considerada ideal para seleção de embriões viáveis aos quinze dias de incubação, quando são comercializados fertilizados.

Estimativa de correlações entre medidas morfométricas, peso do ovo e peso de filhotes de emas criados em cativeiro

Sixty-eight eggs of Rhea had been collected, in the period of 8 August to 13 September of 2003, in Quirinópolis, Goyas, Brazil. The objective was to evaluate the correlation between egg’s weight (PO), morfometrics characteristics: egg’s length (CO), egg’s width (LO), egg’s circumference (CIO), air chamber’s length (CCA), rind’s thickness (EC); birth’s weight (PN) and weight’s equivalence (EP). The egg’s weight had significant correlation (P 001) with CO, LO e CIO; CO presented significant correlation (P 005) com LO e CIO; LO was correlated significantly (P 001) com CIO e (P 005) CCA; the CIO had significant correlation (P > 005) with CCA; CCA had significant correlation (P 001) with EC e PN; the EC presented significant correlation (P 001) with PN and the PN had significant correlation (P 001) with EP. The egg’s weight do not constitute selection characteristic and the air chamber length (CCA) was the considered ideal variable for selection viable embryos to the fifteen days of incubation, when they are commercialized fertilized.

Foram coletados 68 ovos de Ema no período de 8 de agosto a 13 de setembro de 2003, em Quirinópolis, Goiás, Brasil. O objetivo foi avaliar a correlação entre peso do ovo (PO), características morfométricas: comprimento do ovo (CO), largura do ovo (LO), circunferência do ovo (CIO), comprimento da câmara de ar (CCA), espessura da casca (EC); peso ao nascimento (PN) e equivalência de peso (EP). O peso do ovo teve correlação significativa (P 0,01) com CO, LO e CIO; CO apresentou correlação significativa (P 0,05) com LO e CIO; LO foi correlacionada significativamente (P 0,01) com CIO e (P 0,05) CCA; a CIO teve correlação significativa (P > 0,05) com CCA; CCA teve correlação significativa (P 0,01) com EC; a EC apresentou correlação significativa (P 0,01) com PN e PN teve correlação significativa (P 0,01) com EP. Observou-se que o peso do ovo não constitui característica de seleção e o comprimento da câmara de ar (CCA) foi a variável considerada ideal para seleção de embriões viáveis aos quinze dias de incubação, quando são comercializados fertilizados.