Resumo
Purpose: To investigate the microbiological, inflammatory and oxidant effects of adjuvant ozone administration in experimental rat vascular graft infection model which has not been previously investigated. Methods: Forty adult Wistar rats were divided into Sham, Control, Vancomycin, Ozone, Vancomycin+Ozone groups. Grafts were inoculated with Methicillin-resistant Staphylococcus aureus (MRSA) strain and implanted subcutaneously. Rats were treated intraperitoneally with ozone and /or intramuscularly with vancomycin for 10 days. Grafts were evaluated by quantitative bacterial cultures. Blood samples were harvested for determination of thiol-disulphide and cytokine profiles. Results: There was no significant difference in bacterial counts between Control and Ozone Groups. In the Ozone Group median colony count was significantly higher than the Vancomycin and Vancomycin+Ozone Groups. Total thiol and disulphide levels increased and disulphide/native thiol and disulphide/total thiol ratios decreased in Ozone Group significantly. Albumin levels decreased significantly in Vancomycin and Vancomycin+Ozone Groups compared to the Sham Group. IL-1 and TNF-alpha levels significantly increased in infected rats. Decreased levels of VEGF due to infection reversed by ozone therapy in control and vancomycin groups. Conclusions: We didn't observe any benefit of the agent on MRSA elimination in our model. Likewise, effects of ozone on thiol-disulphide homeostasis and inflammatory cytokines were contradictory.(AU)
Assuntos
Animais , Masculino , Adulto , Ratos , Ozônio/administração & dosagem , Ozônio/uso terapêutico , Infecções Estafilocócicas/terapia , Staphylococcus aureus , Vancomicina/administração & dosagem , Vancomicina/uso terapêutico , Enxerto Vascular/efeitos adversos , Compostos de SulfidrilaResumo
A celulite aviária é uma patologia que se desenvolve quando a ave sofre alguma arranhadura e têm-se instalação de microrganismos na área lesionada. Essas lesões ocorrem ainda no período de criação dos frangos nos aviários, visto que estas são expostas a sistemas de criação intensivos. A celulite é uma das maiores causas de condenações de carcaças de frangos no Brasil e as condenações parciais possibilitam a comercialização das partes não afetadas. Entretanto, não se conhece a qualidade desta carne e se a presença da patologia em outro grupo muscular é capaz de afetar as características nutricionais, físico-químicas e a estabilidade oxidativa destas. Neste sentido, o objetivo deste trabalho foi avaliar a composição centesimal: teor de proteína, lipídeos, umidade e cinzas; as características físico-químicas de pH e capacidade de retenção de água (CRA), além da oxidação proteica e lipídica da carne de frangos acometidos por celulite aviária em seus três escores de condenação: leve, moderado e grave, além de grupo controle. Para isso, coletou-se 24 meio peitos de frangos (músculo pectoralis major) e cada tratamento foi composto por seis amostras, sendo as análises de oxidação proteica (carbonila e sulfidrila), de oxidação lipídica (TBARs) e pH realizadas em triplicata e os demais em duplicata. Os resultados foram avaliados por análise de variância em delineamento inteiramente casualizado e pelo pós-teste Scott-Knott (p<0,05). Para as análises de composição centesimal, físico-químicas e de oxidação proteica (carbonila e sulfidrila) não foi encontrado diferença estatística. A única diferença significativa encontrada foi para a oxidação lipídica, onde que com o aumento da severidade da lesão de celulite, aumentou-se os valores de TBARs, mostrando que a estabilidade oxidativa das amostras analisadas foi alterada indiretamente pelas lesões presentes em outro grupo muscular.
Avian cellulite is a pathology that develops when it is scratched and microorganisms are installed in the injured area. These injuries still occur in the broiler breeding period in the aviaries since they are exposed to intensive breeding methods. Cellulite is one of the biggest causes of condemnation of chicken carcasses in Brazil and partial condemnations make it possible the marketing of unaffected parties. However, the quality of this meat is not known and whether the presence of the pathology in another muscle group is able of affecting the nutritional characteristics, physico-chemical properties and their oxidative stability. In this sense, the objective of this work was to evaluate a proximate composition: protein, lipid, moisture and ash content; the physicochemical characteristics of pH and water holding capacity (CRA), in addition to the protein and lipid oxidation of chicken meat affected by avian cellulite in its three scores of condemnation: light, moderate and severe, in addition to the control group. For this, 24 half chicken breasts were collected (pectoralis major muscle) and each treatment consisted of six samples, being analysis of protein oxidation (carbonyl and sulfhydryl), lipid oxidation (TBARs) and pH realized in triplicate and the others in duplicate. The results were obtained by analysis of variance in a completely randomized design and the Scott-Knott post-test (p <0.05). For the analysis of chemical composition, physicochemical and protein oxidation (carbonyl and sulfhydryl), no statistical difference was found. The only significant difference found was for lipid oxidation, where with the increase in the severity of the cellulite lesion, the TBARs values increased, showing that the oxidative stability of the analyzed sample was altered indirectly by the lesions present in a another muscle group.