Oxidative stress caused by lead in the lichen Xanthoria parietina

The assessment of the air quality is a major concern to the current time. The monitoring and maintenance of air quality necessarily pass by detecting and estimating the overall air pollution. The use of lichens must be an assessmenttool to be studied. In our work we were interested about the toxicity of lead on the various parameters of stress in the lichen Xanthoria parietina. For this purpose, lichen thalli have been incubated at lead concentrations of 0, 0.5, 1.0, 5.0 and 10.0 mM, for time scale of 0, 24, 48 and 96 hours. The obtained results showed that lead has an action on the various studied parameters, and the intensity of oxidative stress observed in lichens thalli depends on the concentration, and time of exposure. Lead induced a decrease in chlorophyll and protein contents, and an increase in the contents of catalase, hydrogen peroxide and reduced glutathione. Furthermore, the results also showed that high concentrations of lead caused total destruction of reduced glutathione.(AU)

Supplying Hydrogen Water to Ducks Did Not Influence Ammonia Content and Duck Litter Quality

Drinking hydrogen-rich water shows a remarkable antioxidant effect in preventive and therapeutic applications. However, there is no previous report and information on ammonia (NH3) production and duck litter quality when hydrogen water was supplied to ducks. This study verified the effects of supplying hydrogen water to ducks on NH3 production and duck litter quality in a duck rearing environment. A total of 1,200 0-d-old Pekin ducks were divided into 2 groups of similar body weight (3 replicates with 200 ducks per pen) and used for 42 days. The two groups consisted of general water and hydrogen water in the water supply system, as the control and treatment groups, respectively. There were no statistical differences between two groups for NH3 contents for the five weeks (p>0.05), except for week 6. For litter quality, no effects (p>0.05) between the two water groups were found in the pH, total nitrogen (TN), ammonia-N (VBN), and VFA content of litter. The only significant difference observed in duck litter quality was litter moisture contents (p<0.05). Lastly, mineral and heavy metal contents did not significantly differ between the two water groups. As the first pen trials evaluating the effects of hydrogen water on duck litter, these results verify that supplying hydrogen water to ducks did not influence ammonia and duck litter quality.(AU)

Supplying Hydrogen Water to Ducks Did Not Influence Ammonia Content and Duck Litter Quality

Drinking hydrogen-rich water shows a remarkable antioxidant effect in preventive and therapeutic applications. However, there is no previous report and information on ammonia (NH3) production and duck litter quality when hydrogen water was supplied to ducks. This study verified the effects of supplying hydrogen water to ducks on NH3 production and duck litter quality in a duck rearing environment. A total of 1,200 0-d-old Pekin ducks were divided into 2 groups of similar body weight (3 replicates with 200 ducks per pen) and used for 42 days. The two groups consisted of general water and hydrogen water in the water supply system, as the control and treatment groups, respectively. There were no statistical differences between two groups for NH3 contents for the five weeks (p>0.05), except for week 6. For litter quality, no effects (p>0.05) between the two water groups were found in the pH, total nitrogen (TN), ammonia-N (VBN), and VFA content of litter. The only significant difference observed in duck litter quality was litter moisture contents (p<0.05). Lastly, mineral and heavy metal contents did not significantly differ between the two water groups. As the first pen trials evaluating the effects of hydrogen water on duck litter, these results verify that supplying hydrogen water to ducks did not influence ammonia and duck litter quality.

Evaluating chemicals for inducing budburst in peach orchard grown in subtropical conditions / Avaliação de produtos químicos para indução de brotação em pomar de pessegueiro cultivado em condições subtropicais

The availability of chill hours influences the break dormancy, sprouting and production of temperate fruit trees in different regions. During the winter, the chilling requirements for growing peaches in subtropical locations are restricted, and certain chemicals are required to overcome dormancy and induce budburst. As a result, further information concerning the use of these compounds on stimulating sprouting in peach trees is required. The purpose of this study was to evaluate the ability of hydrogen cyanamide, nitrogen fertilizer, and calcium nitrate to trigger budburst in the 'Douradão' peach orchard, which is in the subtropical area of São Paulo state, Brazil. The following tree chemicals were tested: 0.6 % hydrogen cyanamide, 2.5 % nitrogen fertilizer with 4 % calcium nitrate, 4 % calcium nitrate, and control. The hydrogen cyanamide accelerated, predicted, and focused blooming on plants, along with improving fruit set and output. Furthermore, combining nitrogen fertilizer with calcium nitrate accelerated budburst, which boosted flowering, output per tree, and yield. The spraying of the peach plants with just calcium nitrate produced results like those obtained in the control. The results allow for the use of hydrogen cyanamide or nitrogen fertilizer + calcium nitrate as an option for triggering budburst in Douradão cultivar peach trees under subtropical circumstances, depending on the orchards system management used.(AU)

A disponibilidade de horas de frio influencia a quebra da dormência, brotação e produção de árvores frutíferas de clima temperado em diferentes regiões. Os requerimentos de frio para o cultivo de pessegueiros em regiões subtropicais são limitados durante o inverno e alguns produtos químicos são necessários para superar a dormência e induzir a brotação. Portanto, são necessárias mais informações sobre o uso desses compostos na promoção da brotação em pessegueiros. Este trabalho teve como objetivo avaliar a cianamida hidrogenada, o fertilizante nitrogenado e o nitrato de cálcio para brotação, num pomar de pessegueiro 'Douradão', localizado em região subtropical do estado de São Paulo, Brasil. Para isso, três produtos químicos foram avaliados: 0,6% de cianamida hidrogenada; 2,5% de fertilizante nitrogenado + 4% de nitrato de cálcio; 4% de nitrato de cálcio e o controle. A cianamida hidrogenada aumentou o florescimento das plantas, além de aumentar a frutificação e a produção. Além disso, fertilizante nitrogenado + nitrato de cálcio também proveram a brotação, potencializando o florescimento e a produção das plantas. A pulverização dos pessegueiros somente com nitrato de cálcio apresentou desempenho semelhante ao observado na testemunha. Os resultados permitiram indicar a utilização da cianamida hidrogenada ou fertilizante nitrogenado + nitrato de cálcio, como alternativa para indução de brotação em pessegueiros cultivar Douradão, em condições subtropicais, dependendo do sistema de cultivo adotado nos pomares.(AU)

Gas exchange and growth of soursop under salt stress and H2O2 application methods / Trocas gasosas e crescimento de graviola sob estresse salino e métodos de aplicação de H2O2

The production of soursop seedlings in the Northeast region of Brazil has faced limitations due to the high concentrations of salts in the water, so it is necessary to use techniques that enable its cultivation, and the application of hydrogen peroxide to minimize the deleterious effects of salt stress stands out. In this context, the objective was to evaluate forms of application of hydrogen peroxide as an attenuator of salt stress on the gas exchange and growth of soursop seedlings cv. Morada Nova. The experiment was conducted under greenhouse conditions, in an Regolithic Neosol of sandy loam texture, from the municipality of Lagoa Seca - PB. A completely randomized design was used in a 5 × 4 factorial arrangement, whose treatments resulted from the combination of five levels of electrical conductivity of irrigation water - ECw (0.6 - control, 1.2, 1.8, 2.4 and 3.0 dS m-1) and four forms of hydrogen peroxide application (M1 - without H2O2 application, M2 - application by seed soaking, M3 - application by foliar spraying, and M4 - application by seed soaking + foliar spraying), with four replicates and two plants per experimental unit, totaling 160 plants. The concentration of H2O2 used in the different forms of application was 20 µM. Irrigation water salinity from 0.6 dS m-1 reduced the gas exchange and growth of soursop. The method of H2O2 application by foliar spraying minimized the effects of salt stress on gas exchange. The method of H2O2 application by seed soaking reduced the effect of salt stress on the growth of soursop at 85 days after sowing.

A produção de mudas de gravioleira na região semiárida do Brasil tem enfrentado limitações devido às elevadas concentrações de sais nas águas, sendo necessário o uso de técnicas que viabilizem seu cultivo, destacando-se a aplicação do peróxido de hidrogênio. Neste contexto, objetivou-se avaliar as formas de aplicação do peróxido de hidrogênio como atenuante do estresse salino nas trocas gasosas e no crescimento de mudas de gravioleira cv. Morada Nova. O estudo foi conduzido sob condições de casa de vegetação, em um Neossolo Regolítico de textura franco-arenosa, utilizando-se o delineamento inteiramente casualizado, no arranjo fatorial 5 × 4, cujos tratamentos resultaram da combinação de cinco níveis de condutividade elétrica da água de irrigação - CEa (0,6 - testemunha; 1,2; 1,8; 2,4 e 3,0 dS m-1) e quatro formas de aplicação de peróxido de hidrogênio (M1- sem aplicação de H2O2, M2 - aplicação via embebição das sementes, M3 -aplicação por pulverização foliar e M4 - aplicação via embebição das sementes + pulverização foliar), com quatro repetições e duas plantas por unidade experimental, perfazendo o total de 160 plantas. A concentração de H2O2 utilizada nas diferentes formas de aplicação foi de 20 µM. A salinidade da água de irrigação a partir de 0,6 dS m-1 diminuiu as trocas gasosas e o crescimento das plantas de gravioleira. O método de aplicação do H2O2 via pulverização foliar minimizou o efeito do estresse salino sobre as trocas gasosas. O método de aplicação do H2O2 via embebição das sementes diminuiu o estresse salino no crescimento de gravioleira aos 85 dias após o semeio.

Effect of pH soil and irrigation regimes on management of potato scab

ABSTRACT Potato scab caused by different species of phytopathogenic Streptomyces is considered one of the main bacterial diseases of economic crop importance worldwide. Several studies are being carried out in order to control the disease, but until now, there is no efficient way to do this. Some management strategies have been investigated including application of chemical and biological products and utilization of resistant cultivars of potato but there are few reports about the impact of pH and irrigation regimes on the disease. The present study aimed to evaluate the effects of these last two factors on the incidence and severity of potato scab caused by S. scabiei, S. acidiscabies, Streptomyces sp., S. caviscabies and S. europaeiscabiei in assays at pH 4.0, 4.5, 5.0, 5.5, 6.5 and 7.5; and irrigation regimes of once a week, alternate days and daily in greenhouse conditions. The experimental design for the pH tests was randomized blocks arranged in a 5x2 factorial scheme, with 5 replications and 3x2 for the irrigation regimes with 5 replications. The pH tests showed significant differences between the treatments and pH 4,0 - 4,5 presented lower incidence and severity of the disease for the most species tested but no significant differences were observed between the irrigation regimes. The soil acidification is considered a classic strategy for management of the disease and the results obtained herein corroborated this hypothesis.

Hydrogen-rich saline alleviates early brain injury through regulating of ER stress and autophagy after experimental subarachnoid hemorrhage

ABSTRACT Purpose: Subarachnoid hemorrhage (SAH) is a common complication of cerebral vascular disease. Hydrogen has been reported to alleviate early brain injury (EBI) through oxidative stress injury, reactive oxygen species (ROS), and autophagy. Autophagy is a programmed cell death mechanism that plays a vital role in neuronal cell death after SAH. However, the precise role of autophagy in hydrogen-mediated neuroprotection following SAH has not been confirmed. Methods: In the present study, the objective was to investigate the neuroprotective effects and potential molecular mechanisms of hydrogen-rich saline in SAH-induced EBI by regulating neural autophagy in the C57BL/6 mice model. Mortality, neurological score, brain water content, ROS, malondialdehyde (MDA), and neuronal death were evaluated. Results: The results show that hydrogen-rich saline treatment markedly increased the survival rate and neurological score, increased neuron survival, downregulated the autophagy protein expression of Beclin-1 and LC3, and endoplasmic reticulum (ER) stress. That indicates that hydrogen-rich saline-mediated inhibition of autophagy and ER stress ameliorate neuronal death after SAH. The neuroprotective capacity of hydrogen-rich saline is partly dependent on the ROS/Nrf2/heme oxygenase-1 (HO-1) signaling pathway. Conclusions: The results of this study demonstrate that hydrogen-rich saline improves neurological outcomes in mice and reduces neuronal death by protecting against neural autophagy and ER stress.

Effect of pH soil and irrigation regimes on management of potato scab

Potato scab caused by different species of phytopathogenic Streptomyces is considered one of the main bacterial diseases of economic crop importance worldwide. Several studies are being carried out in order to control the disease, but until now, there is no efficient way to do this. Some management strategies have been investigated including application of chemical and biological products and utilization of resistant cultivars of potato but there are few reports about the impact of pH and irrigation regimes on the disease. The present study aimed to evaluate the effects of these last two factors on the incidence and severity of potato scab caused by S. scabiei, S. acidiscabies, Streptomyces sp., S. caviscabies and S. europaeiscabiei in assays at pH 4.0, 4.5, 5.0, 5.5, 6.5 and 7.5; and irrigation regimes of once a week, alternate days and daily in greenhouse conditions. The experimental design for the pH tests was randomized blocks arranged in a 5x2 factorial scheme, with 5 replications and 3x2 for the irrigation regimes with 5 replications. The pH tests showed significant differences between the treatments and pH 4,0 - 4,5 presented lower incidence and severity of the disease for the most species tested but no significant differences were observed between the irrigation regimes. The soil acidification is considered a classic strategy for management of the disease and the results obtained herein corroborated this hypothesis.

Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations

Microbial proteases, especially from Bacillus spp., have been widely exploited for a broad variety of applications, such as the improvement of the cleaning efficiency of conventional detergents. In this work, the statistical design of the experiment was used to optimize the concentrations of a three-component mixture: Bacillus licheniformis SMIA-2 protease, Linear Alkylbenzene Sulphate and hydrogen peroxide, in an attempt to prepare an environmentally correct cleaning formulation. The results demonstrated that the combination of 1% (w/v) protease with 1.5% (w/v) LAS and 1% (w/v) H2O2 was effective in removing blood from cloth pieces and that a protease concentration decrease from 1.0% to 0.5% (w/v) would not have a signi&64257;cant impact on percent blood removal if LAS concentrations between 1.5-2.0% (w/v) in combination with lower (<0.5%, w/v) concentrations of H2O2 were used. Thus, the protease from Bacillus licheniformis SMIA-2 can be effectively incorporated into cleaning formulations together with LAS and hydrogen peroxide to formulate more sustainable detergents.

Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations

Microbial proteases, especially from Bacillus spp., have been widely exploited for a broad variety of applications, such as the improvement of the cleaning efficiency of conventional detergents. In this work, the statistical design of the experiment was used to optimize the concentrations of a three-component mixture: Bacillus licheniformis SMIA-2 protease, Linear Alkylbenzene Sulphate and hydrogen peroxide, in an attempt to prepare an environmentally correct cleaning formulation. The results demonstrated that the combination of 1% (w/v) protease with 1.5% (w/v) LAS and 1% (w/v) H2O2 was effective in removing blood from cloth pieces and that a protease concentration decrease from 1.0% to 0.5% (w/v) would not have a signi&64257;cant impact on percent blood removal if LAS concentrations between 1.5-2.0% (w/v) in combination with lower (<0.5%, w/v) concentrations of H2O2 were used. Thus, the protease from Bacillus licheniformis SMIA-2 can be effectively incorporated into cleaning formulations together with LAS and hydrogen peroxide to formulate more sustainable detergents.(AU)

Foliar application of H2O2 as salt stress attenuator in 'BRS Rubi do Cerrado' sour passion fruit / Aplicação foliar de H2O2 como atenuante do estresse salino em maracujazeiro-azedo 'BRS Rubi do Cerrado'

Irrigation with saline water causes a reduction in yield, especially in semi-arid regions. Cultivation strategies have been developed to mitigate salt stress on plants, such as the use of hydrogen peroxide. The objective of this study was to evaluate the attenuating effect of hydrogen peroxide on the gas exchange and growth of 'BRS Rubi do Cerrado' sour passion fruit cultivated under irrigation with saline water. The design was completely randomized in split-plot plots, with water salinity levels ECw (0.6, 1.2, 1.8, 2.4, and 3.0 dS m-1) considered the plots and the concentrations of hydrogen peroxide H2O2 (0, 15, 30, and 45 µM) considered the subplots, with three replicates. Gas exchange (stomatal conductance, transpiration, CO2 assimilation rate, intercellular CO2 concentration, instantaneous water use efficiency, and instantaneous carboxylation efficiency), and absolute and relative growth rates in stem diameter were evaluated. An increase in irrigation water salinity from 0.6 dS m-1 reduced gas exchange, and exogenous application of hydrogen peroxide did not promote a significant effect on gas exchange. However, foliar application of hydrogen peroxide at 15 µM increased the growth of 'BRS Rubi do Cerrado' sour passion fruit.(AU)

A irrigação com águas salinas causa redução na produtividade, principalmente nas regiões semiáridas. Estratégias de cultivo vêm sendo desenvolvidas, a exemplo uso de peróxido de hidrogênio para mitigar o estresse salino sobre as plantas. Objetivou-se avaliar o efeito atenuante do peróxido de hidrogênio sobre as trocas gasosas e o crescimento do maracujazeiro-azedo 'BRS Rubi do Cerrado' cultivado sob irrigação com águas salinas. O delineamento foi inteiramente casualizados em parcelas subdivididas, sendo os níveis de salinidade da água - CEa (0,6, 1,2, 1,8, 2,4 e 3,0 dS m-1) consideradas as parcelas, e as concentrações de peróxido de hidrogênio ­ H2O2 (0, 15, 30 e 45 µM) as subparcelas, com três repetições. Foram avaliadas as trocas gasosas (condutância estomática, transpiração, taxa de assimilação de CO2, concentração intercelular de CO2, eficiência instantânea no uso da água e eficiência instantânea de carboxilação), e a taxa de crescimento absoluto e relativo do caule. O aumento na salinidade da água de irrigação a partir da salinidade de 0,6 dS m-1 reduziu as trocas gasosas; a aplicação exógena de peróxido de hidrogênio não promoveu efeito significativos sob as trocas gasosas. A aplicação foliar de 15 µM de peróxido de hidrogênio aumentou o crescimento do maracujazeiro-azedo 'BRS Rubi do Cerrado'.(AU)

Oxidative stress and antioxidant defense in detoxification systems of snake venom-induced toxicity

Snakebites remain a major life-threatening event worldwide. It is still difficult to make a positive identification of snake species by clinicians in both Western medicine and Chinese medicine. The main reason for this is a shortage of diagnostic biomarkers and lack of knowledge about pathways of venom-induced toxicity. In traditional Chinese medicine, snakebites are considered to be treated with wind, fire, and wind-fire toxin, but additional studies are required. Methods: Cases of snakebite seen at the Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine were grouped as follows: fire toxin - including four cases of bites by Agkistrodon acutus and three bites by Trimeresurus stejnegeri - and wind-fire toxin - four cases of bites by vipers and three bites by cobras. Serum protein quantification was performed using LC-MS/MS. Differential abundance proteins (DAPs) were identified from comparison of snakebites of each snake species and healthy controls. The protein interaction network was constructed using STITCH database. Results: Principal component analysis and hierarchical clustering of 474 unique proteins exhibited protein expression profiles of wind-fire toxins that are distinct from that of fire toxins. Ninety-three DAPs were identified in each snakebite subgroup as compared with healthy control, of which 38 proteins were found to have significantly different expression levels and 55 proteins displayed no expression in one subgroup, by subgroup comparison. GO analysis revealed that the DAPs participated in bicarbonate/oxygen transport and hydrogen peroxide catabolic process, and affected carbon-oxygen lyase activity and heme binding. Thirty DAPs directly or indirectly acted on hydrogen peroxide in the interaction network of proteins and drug compounds. The network was clustered into four groups: lipid metabolism and transport; IGF-mediated growth; oxygen transport; and innate immunity. Conclusions: Our results show that the pathways of snake venom-induced toxicity may form a protein network of antioxidant defense by regulating oxidative stress through interaction with hydrogen peroxide.(AU)

Oxidative stress and antioxidant defense in detoxification systems of snake venom-induced toxicity

Snakebites remain a major life-threatening event worldwide. It is still difficult to make a positive identification of snake species by clinicians in both Western medicine and Chinese medicine. The main reason for this is a shortage of diagnostic biomarkers and lack of knowledge about pathways of venom-induced toxicity. In traditional Chinese medicine, snakebites are considered to be treated with wind, fire, and wind-fire toxin, but additional studies are required. Methods: Cases of snakebite seen at the Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine were grouped as follows: fire toxin - including four cases of bites by Agkistrodon acutus and three bites by Trimeresurus stejnegeri - and wind-fire toxin - four cases of bites by vipers and three bites by cobras. Serum protein quantification was performed using LC-MS/MS. Differential abundance proteins (DAPs) were identified from comparison of snakebites of each snake species and healthy controls. The protein interaction network was constructed using STITCH database. Results: Principal component analysis and hierarchical clustering of 474 unique proteins exhibited protein expression profiles of wind-fire toxins that are distinct from that of fire toxins. Ninety-three DAPs were identified in each snakebite subgroup as compared with healthy control, of which 38 proteins were found to have significantly different expression levels and 55 proteins displayed no expression in one subgroup, by subgroup comparison. GO analysis revealed that the DAPs participated in bicarbonate/oxygen transport and hydrogen peroxide catabolic process, and affected carbon-oxygen lyase activity and heme binding. Thirty DAPs directly or indirectly acted on hydrogen peroxide in the interaction network of proteins and drug compounds. The network was clustered into four groups: lipid metabolism and transport; IGF-mediated growth; oxygen transport; and innate immunity. Conclusions: Our results show that the pathways of snake venom-induced toxicity may form a protein network of antioxidant defense by regulating oxidative stress through interaction with hydrogen peroxide.(AU)

Sprouting, phenology, and maturation of the Italian grapevine Fiano in Campo Largo, PR, Brazil / Brotação, fenologia e maturação da videira italiana Fiano em Campo Largo, PR, Brasil

Despite the expansion of viticulture in Brazil, the lack of chilling hours is a factor that requires the use of growth inducers to promote sprouting. Erger™ is a product of low toxicity and efficient in the bud induction of apple. However, there is a lack of studies testing such a product on the grapevine. This research was carried out to evaluate the sprouting and phenological cycle of ‘Fiano’ grapevine after Erger™ and calcium nitrate application in comparison to hydrogen cyanamide, as well as characterizing its production in Brazil. The experiment was carried out in Campo Largo, PR, under a trellis system, with plants spaced at 1.25 × 2.7 meters. The experimental design was a randomized complete block design with four replications and three plants per plot. Different concentrations of Erger™ and calcium nitrate (3%, 5%, 7%, and control) were tested compared to the standard dose of Dormex™ (4%) to induce sprouting in ‘Fiano’ grapevine, while phenological aspects of this cultivar were evaluated following the BBCH scale. Soluble solids content, titratable acidity, and pH were evaluated weekly after the beginning of berry maturation. The main biometric and qualitative characteristics of bunches were evaluated at harvest. All treatments presented satisfactory sprouting rates in 2014, but a lower cold accumulation was observed in 2015, and Erger™ application significantly increased sprouting when compared to the control. Plants treated with Erger™ had a delay in sprouting in the two years of study, requiring more time to reach a satisfactory percentage of open buds. The anticipation of sprouting was also observed in all treatments in the second agricultural season due to higher temperatures in relation to the previous year when the occurrence of low temperatures led to a longer time to start bud opening, which was related to ecodormancy. The cultivar Fiano is productive and adapted to the conditions of Campo Largo, PR, Brazil.

Apesar da expansão da viticultura no Brasil, a falta de horas de frio é um fator que obriga o uso de indutores de crescimento para promover a brotação. O Erger® é um produto de baixa toxicidade e eficiente na indução de brotação de macieira, no entanto, há carência de trabalhos testando tal produto com videira. Esta pesquisa foi realizada com o objetivo de avaliar a brotação e o ciclo fenológico da videira Fiano após aplicação de Erger® e nitrato de cálcio em comparação com a cianamida hidrogenada e caracterizar a produção desta cultivar no Brasil. O experimento foi realizado num parreiral localizado em Campo Largo-PR, conduzido em sistema de espaldeira, com plantas espaçadas em 1,25 x 2,7 metros. O delineamento experimental utilizado foi em blocos ao acaso, com 4 repetições e 3 plantas por parcela, onde foram testadas diferentes concentrações de Erger® e nitrato de cálcio (3%, 5%, 7% e testemunha) comparadas à dose padrão de Dormex® (4%) para induzir a brotação de videiras da cultivar Fiano, além de acompanhar aspectos fenológicos seguindo a escala BBCH. Após o início da maturação das bagas, foi avaliado semanalmente o teor de sólidos solúveis, a acidez titulável e o pH. Na colheita foram avaliadas as principais características biométricas e qualitativas dos cachos. Observaram-se taxas de brotação satisfatórias em todos os tratamentos em 2014, mas em 2015 houve menor acúmulo de frio e a aplicação de Erger® aumentou significativamente a brotação em relação a testemunha. Nos dois anos de estudo, as plantas tratadas com Erger® sofreram um atraso na brotação, exigindo mais tempo para atingir uma porcentagem satisfatória de gemas abertas. Também foi observada uma antecipação da brotação em todos os tratamentos na segunda safra em virtude de temperaturas mais elevadas em relação ao ano anterior, quando a ocorrência de temperaturas baixas ocasionou maior tempo para iniciar a abertura de gemas em decorrência da instalação de uma...

Sprouting, phenology, and maturation of the Italian grapevine Fiano in Campo Largo, PR, Brazil / Brotação, fenologia e maturação da videira italiana Fiano em Campo Largo, PR, Brasil

Despite the expansion of viticulture in Brazil, the lack of chilling hours is a factor that requires the use of growth inducers to promote sprouting. Erger™ is a product of low toxicity and efficient in the bud induction of apple. However, there is a lack of studies testing such a product on the grapevine. This research was carried out to evaluate the sprouting and phenological cycle of ‘Fiano grapevine after Erger™ and calcium nitrate application in comparison to hydrogen cyanamide, as well as characterizing its production in Brazil. The experiment was carried out in Campo Largo, PR, under a trellis system, with plants spaced at 1.25 × 2.7 meters. The experimental design was a randomized complete block design with four replications and three plants per plot. Different concentrations of Erger™ and calcium nitrate (3%, 5%, 7%, and control) were tested compared to the standard dose of Dormex™ (4%) to induce sprouting in ‘Fiano grapevine, while phenological aspects of this cultivar were evaluated following the BBCH scale. Soluble solids content, titratable acidity, and pH were evaluated weekly after the beginning of berry maturation. The main biometric and qualitative characteristics of bunches were evaluated at harvest. All treatments presented satisfactory sprouting rates in 2014, but a lower cold accumulation was observed in 2015, and Erger™ application significantly increased sprouting when compared to the control. Plants treated with Erger™ had a delay in sprouting in the two years of study, requiring more time to reach a satisfactory percentage of open buds. The anticipation of sprouting was also observed in all treatments in the second agricultural season due to higher temperatures in relation to the previous year when the occurrence of low temperatures led to a longer time to start bud opening, which was related to ecodormancy. The cultivar Fiano is productive and adapted to the conditions of Campo Largo, PR, Brazil.(AU)

Apesar da expansão da viticultura no Brasil, a falta de horas de frio é um fator que obriga o uso de indutores de crescimento para promover a brotação. O Erger® é um produto de baixa toxicidade e eficiente na indução de brotação de macieira, no entanto, há carência de trabalhos testando tal produto com videira. Esta pesquisa foi realizada com o objetivo de avaliar a brotação e o ciclo fenológico da videira Fiano após aplicação de Erger® e nitrato de cálcio em comparação com a cianamida hidrogenada e caracterizar a produção desta cultivar no Brasil. O experimento foi realizado num parreiral localizado em Campo Largo-PR, conduzido em sistema de espaldeira, com plantas espaçadas em 1,25 x 2,7 metros. O delineamento experimental utilizado foi em blocos ao acaso, com 4 repetições e 3 plantas por parcela, onde foram testadas diferentes concentrações de Erger® e nitrato de cálcio (3%, 5%, 7% e testemunha) comparadas à dose padrão de Dormex® (4%) para induzir a brotação de videiras da cultivar Fiano, além de acompanhar aspectos fenológicos seguindo a escala BBCH. Após o início da maturação das bagas, foi avaliado semanalmente o teor de sólidos solúveis, a acidez titulável e o pH. Na colheita foram avaliadas as principais características biométricas e qualitativas dos cachos. Observaram-se taxas de brotação satisfatórias em todos os tratamentos em 2014, mas em 2015 houve menor acúmulo de frio e a aplicação de Erger® aumentou significativamente a brotação em relação a testemunha. Nos dois anos de estudo, as plantas tratadas com Erger® sofreram um atraso na brotação, exigindo mais tempo para atingir uma porcentagem satisfatória de gemas abertas. Também foi observada uma antecipação da brotação em todos os tratamentos na segunda safra em virtude de temperaturas mais elevadas em relação ao ano anterior, quando a ocorrência de temperaturas baixas ocasionou maior tempo para iniciar a abertura de gemas em decorrência da instalação de uma...(AU)

Losses, chemical composition and aerobic stability of rye silage cv. Temprano subjected to different pre-flowering cuttings with or without fungicide / Perdas, composição química e estabilidade aeróbia da silagem de centeio cv. Temprano submetido a diferentes sistemas de corte com ou sem fungicida no pré-florescimento

The present study evaluated the fermentation, chemical characteristics and ruminal disappearance of dry matter of silages of rye cv. Temprano harvested at the stage of floury grain, managed with or without cutting at the vegetative stage and with or without application of Fluxapyroxad + Pyraclostrobinbased fungicide at the pre-flowering phenological stage. This was a 2x2 factorial randomized block experimental design, with 2 cutting regimes (0 and 1 cut) and 2 application management (with and without fungicide), with six replications, where each repetition is represented by a plot of 9.45m², with the treatments SC-SF: without cut at the vegetative stage and without fungicide application; SC-CF: without cut at the vegetative stage with fungicide application; UC-SF: a cut at the vegetative stage with pre-flowering fungicide application; UC-CF: a cut at the vegetative stage and without fungicide application. UC-CF silages were better in the general context, due to the better fiber composition with an average 6.75% reduction in neutral detergent fiber and acid detergent fiber and a 17.10% reduction in lignin content, but, due to its higher DM content, it presented a less efficient fermentation process in terms of organic acid concentration and aerobic stability. However, it did not change the DM losses or ruminal degradability, and there is also the use of DM harvested at the vegetative stage that certainly has a superior quality. The use of fungicide provided only better dry matter index and better fiber composition and improved the fermentation process resulting in lower DM losses without altering the aerobic stability of silages.(AU)

Objetivou-se avaliar as características fermentativas, bromatológicas e o desaparecimento ruminal da matéria seca das silagens de Centeio cv. Temprano colhidas no estádio de grão farináceo, manejado com ou sem corte no estádio vegetativo e com ou sem aplicação de fungicida a base de Fluxapiroxade + Piraclostrobina no estádio fenológico de pré-florescimento. O delineamento experimental foi o de blocos ao acaso em esquema fatorial 2x2, sendo 2 regimes de cortes (0 e 1 corte) e 2 manejos de aplicação (com e sem fungicida), com seis repetições, onde cada repetição é representada por uma parcela de 9,45m², sendo os tratamentos SC-SF: sem corte no vegetativo e sem aplicação de fungicida; SC-CF: sem corte no vegetativo com aplicação de fungicida; UC-SF: um corte no vegetativo com aplicação de fungicida no pré-florescimento; UC-CF: um corte no vegetativo e sem aplicação de fungicida; As silagens do tratamento UC-CF apresentaram-se melhores no contexto geral, devido a melhor composição da fibra com redução de 6,75% em média nos teores de fibra em detergente neutro e fibra em detergente ácido e uma redução de 17,10% nos teores de lignina, no entanto devido ao seu maior teor de MS apresentou um processo fermentativo menos eficiente em termos de concentração de ácidos orgânicos e de estabilidade aeróbia, porém, o mesmo não alterou as perdas de MS nem degradabilidade ruminal, atribuído a isso ainda tem-se o aproveitamento da MS colhida no estádio vegetativo que tem certamente uma qualidade superior. A utilização de fungicida proporcionou apenas melhores índices de matéria seca e melhor composição da fibra e, melhorou o processo fermentativo gerando menores perdas de MS sem alterar a estabilidade aeróbica das silagens.(AU)

Budburst and flowering intensity by the spraying of dormancy-breaking products in 'Eva' apple trees / Brotação e intensidade de floração pela aplicação de indutores de brotação em macieiras 'Eva'

Spraying of dormancy-breaking chemicals is a mandatory procedure to produce temperate fruits in low-chill regions. Although hydrogen cyanamide (HC) + mineral oil (MO) show efficiency enhancing budburst, the usage of HC is restricted in some countries due to its toxicity. Therefore, this research aimed to evaluate the efficiency of spraying different dormancy-breaking agents on ‘Eva apple tree buds, under the conditions of the Depressão Central of Rio Grande do Sul, Brazil. Different doses of Erger® (0, 2, 3, 4, 5%) + 3% Ca(NO3)2 were tested and compared with MO (4%) or MO (4%) + HC (0.6%). Budburst rate of apical and axillary buds, physiological alterations in buds, return bloom, yield and fruit weight were evaluated. Erger® treatments efficiently enhanced budburst, with a result equivalent to HC + MO treatment. The budburst rate increased as the Erger® dose increased, also causing the increment of the return bloom in the following year. However, doses of 4 and 5% caused the death of shoots. The activity of the peroxidases and the content of H2O2 in the buds were affected by the treatments. Yield and fruit mass were different in response to treatments, although the effect varied between years. Erger® + Ca(NO3)2 spraying increase budburst in apical and axillary buds of ‘Eva apple tree in low-chill conditions and doses up to 3% of the commercial product do not cause toxicity.(AU)

A aplicação de indutores de brotação é uma prática fundamental para a produção de frutíferas temperadas em regiões com menor quantidade de horas de frio. Apesar de cianamida hidrogena (CH) + óleo mineral (OM) apresentarem eficiência na indução da brotação de gemas de macieira, a toxicidade de CH ao aplicador resultou em restrições ao seu uso em alguns países. Assim, o objetivo do trabalho foi avaliar a eficiência da aplicação de diferentes indutores de brotação de gemas na macieira ‘Eva, nas condições da Depressão Central do RS. Testaram-se diferentes doses de Erger® (0, 2, 3, 4, 5%) + 3% Ca(NO3)2, comparados com OM (4%) ou OM (4%) + CH (0,6%). Foram avaliados a taxa de brotação de gemas apicais e axilares, alterações fisiológicas nas gemas, o retorno floral, a produção e a massa dos frutos. Os tratamentos de Erger® foram eficientes em aumentar a brotação de gemas, com resultados equivalentes ao tratamento CH + OM. A taxa de brotação de gemas aumentou em resposta ao aumento na dose de Erger®, provocando também incremento no retorno floral no ano seguinte, no entanto doses de 4 e 5% provocaram morte de ramos. A atividade de peroxidases e os teores de peróxidos de hidrogênio nas gemas apicais foram afetadas pelos tratamentos. A produção e a massa média dos frutos diferiram entre tratamentos, porém o efeito variou entre as safras. A aplicação de Erger® + Ca(NO3)2 aumenta a brotação de gemas apicais e axilares em macieiras ‘Eva em condição de baixo acúmulo de frio e doses de até 3% do produto comercial não causam toxidez.(AU)

Budburst and flowering intensity by the spraying of dormancy-breaking products in 'Eva' apple trees / Brotação e intensidade de floração pela aplicação de indutores de brotação em macieiras 'Eva'

Spraying of dormancy-breaking chemicals is a mandatory procedure to produce temperate fruits in low-chill regions. Although hydrogen cyanamide (HC) + mineral oil (MO) show efficiency enhancing budburst, the usage of HC is restricted in some countries due to its toxicity. Therefore, this research aimed to evaluate the efficiency of spraying different dormancy-breaking agents on ‘Eva’ apple tree buds, under the conditions of the Depressão Central of Rio Grande do Sul, Brazil. Different doses of Erger® (0, 2, 3, 4, 5%) + 3% Ca(NO3)2 were tested and compared with MO (4%) or MO (4%) + HC (0.6%). Budburst rate of apical and axillary buds, physiological alterations in buds, return bloom, yield and fruit weight were evaluated. Erger® treatments efficiently enhanced budburst, with a result equivalent to HC + MO treatment. The budburst rate increased as the Erger® dose increased, also causing the increment of the return bloom in the following year. However, doses of 4 and 5% caused the death of shoots. The activity of the peroxidases and the content of H2O2 in the buds were affected by the treatments. Yield and fruit mass were different in response to treatments, although the effect varied between years. Erger® + Ca(NO3)2 spraying increase budburst in apical and axillary buds of ‘Eva’ apple tree in low-chill conditions and doses up to 3% of the commercial product do not cause toxicity.

A aplicação de indutores de brotação é uma prática fundamental para a produção de frutíferas temperadas em regiões com menor quantidade de horas de frio. Apesar de cianamida hidrogena (CH) + óleo mineral (OM) apresentarem eficiência na indução da brotação de gemas de macieira, a toxicidade de CH ao aplicador resultou em restrições ao seu uso em alguns países. Assim, o objetivo do trabalho foi avaliar a eficiência da aplicação de diferentes indutores de brotação de gemas na macieira ‘Eva’, nas condições da Depressão Central do RS. Testaram-se diferentes doses de Erger® (0, 2, 3, 4, 5%) + 3% Ca(NO3)2, comparados com OM (4%) ou OM (4%) + CH (0,6%). Foram avaliados a taxa de brotação de gemas apicais e axilares, alterações fisiológicas nas gemas, o retorno floral, a produção e a massa dos frutos. Os tratamentos de Erger® foram eficientes em aumentar a brotação de gemas, com resultados equivalentes ao tratamento CH + OM. A taxa de brotação de gemas aumentou em resposta ao aumento na dose de Erger®, provocando também incremento no retorno floral no ano seguinte, no entanto doses de 4 e 5% provocaram morte de ramos. A atividade de peroxidases e os teores de peróxidos de hidrogênio nas gemas apicais foram afetadas pelos tratamentos. A produção e a massa média dos frutos diferiram entre tratamentos, porém o efeito variou entre as safras. A aplicação de Erger® + Ca(NO3)2 aumenta a brotação de gemas apicais e axilares em macieiras ‘Eva’ em condição de baixo acúmulo de frio e doses de até 3% do produto comercial não causam toxidez.

Comparison of the efficacy of hydrogen peroxide and salt for control of fungal infections on brown trout (Salmo trutta) eggs

Background: Fungal infections can cause serious problems infecting fish eggs, especially unfertilized or dead eggs. In the past, this problem was solved by using very effective chemicals such as malachite green and formalin. But, due to its toxicity and carcinogenicity, malachite green was banned for use in fish intended for human consumption. Formalin also has been banned in most countries. Chemicals and drugs recommended for use to treat fungal infections are hydrogen peroxide, salt, potassium permanganate etc. The goal of the present study was to determine and compare the efficacy of antifungal effects of hydrogen peroxide and sodium chloride on brown trout eggs.Materials, Methods & Results: The experiment was conducted in the brown trout hatchery, Šipovo, Bosnia and Herzegovina. The experimental groups contained 500 and 1,000 mg/L of hydrogen peroxide with 15 and 30 min of exposition; 1 and 2.5% of sodium chloride with 15 and 30 min of exposition and a negative control group (no chemical treatment). The treatment concentrations were calculated and prepared from hydrogen peroxide of 35% active ingredient, and sodium chloride (sterilized) of 100% active ingredient. Eggs for the study were spawned from 11 females and 4 males. The first treatment was performed on the fourth day, and each next treatment was performed at 3-day intervals. Six treatments were administered until the 19th day after the fertilization. The treatment of the eggs was provided until the eggs reached the eyed stage. The effectiveness of the chemical treatments was measured by a hatch rate. There was a significant difference between all treated groups and the negative control group (P < 0.05). Hydrogen peroxide with a concentration of 500 mg/L for 30 min was the most effective and demonstrated a higher hatching rate (75.7%). Sodium chloride treatments resulted in statistically significantly lower hatching rates than hydrogen peroxide treatments.[...]

Comparison of the efficacy of hydrogen peroxide and salt for control of fungal infections on brown trout (Salmo trutta) eggs

Background: Fungal infections can cause serious problems infecting fish eggs, especially unfertilized or dead eggs. In the past, this problem was solved by using very effective chemicals such as malachite green and formalin. But, due to its toxicity and carcinogenicity, malachite green was banned for use in fish intended for human consumption. Formalin also has been banned in most countries. Chemicals and drugs recommended for use to treat fungal infections are hydrogen peroxide, salt, potassium permanganate etc. The goal of the present study was to determine and compare the efficacy of antifungal effects of hydrogen peroxide and sodium chloride on brown trout eggs.Materials, Methods & Results: The experiment was conducted in the brown trout hatchery, Šipovo, Bosnia and Herzegovina. The experimental groups contained 500 and 1,000 mg/L of hydrogen peroxide with 15 and 30 min of exposition; 1 and 2.5% of sodium chloride with 15 and 30 min of exposition and a negative control group (no chemical treatment). The treatment concentrations were calculated and prepared from hydrogen peroxide of 35% active ingredient, and sodium chloride (sterilized) of 100% active ingredient. Eggs for the study were spawned from 11 females and 4 males. The first treatment was performed on the fourth day, and each next treatment was performed at 3-day intervals. Six treatments were administered until the 19th day after the fertilization. The treatment of the eggs was provided until the eggs reached the eyed stage. The effectiveness of the chemical treatments was measured by a hatch rate. There was a significant difference between all treated groups and the negative control group (P < 0.05). Hydrogen peroxide with a concentration of 500 mg/L for 30 min was the most effective and demonstrated a higher hatching rate (75.7%). Sodium chloride treatments resulted in statistically significantly lower hatching rates than hydrogen peroxide treatments.[...](AU)