Resumo
Mycobacteriosis was detected in seven out of one Atlantic mackerel (Scomber scombrus) that was purchased for human consumption from a fish market. The fish was apparently healthy but during cleaning, several granulomatous foci were noticed in the visceral organs. Histopathological examination of the lesions revealed numerous foci characterized by caseous necrosis in the center of the lesion surrounded by epithelioid giant cells. Ziehl-Neelsen staining revealed the presence of rod-shaped, acid-fast bacteria. Furthermore, immunohistochemical examination revealed the presence of a protein of mycobacterial origin in giant cells and macrophages. Based on gross and microscopic findings, mycobacteriosis was diagnosed. This report showed that due to its zoonotic potential, mycobacteriosis should be considered even in healthy-appearing fishes for human consumption.
A micobacteriose foi detectada em uma de sete cavalas do Atlântico (Scomber scombrus) que foi comprada para consumo humano em um mercado de peixe. O peixe era aparentemente saudável, mas durante a limpeza, vários focos granulomatosos foram notados nos órgãos viscerais. O exame histopatológico das lesões revelou numerosos focos caracterizados por necrose caseosa no centro da lesão circundada por células gigantes epitelioides. A coloração de Ziehl-Neelsen revelou a presença de bactérias em forma de bastonete, com rápida acidez. Além disso, o exame imunohistoquímico revelou a presença de uma proteína de origem micobacteriana em células gigantes e macrófagos. Com base em achados grosseiros e microscópicos, a micobacteriose foi diagnosticada. Este relatório mostrou que devido a seu potencial zoonótico, a micobacteriose deve ser considerada mesmo em peixes saudáveis para consumo humano.
Assuntos
Animais , Perciformes/microbiologia , Infecções por Mycobacterium/diagnóstico , Infecções por Mycobacterium/veterinária , Imuno-Histoquímica/veterinária , Zoonoses Bacterianas/diagnósticoResumo
Foi investigado o valor diagnóstico da resposta alérgica cutânea em leitões experimentalmente sensibilizados, pela via intramuscular, com suspensões oleosas de Mycobacterium bovis ou M. avium inativados pelo calor.Foram utilizados 91 animais, divididos em quatro grupos: grupos A e B, cada um com 25 indivíduos, grupos C e D com 21 e 20 indivíduos respectivamente, balanceando-se as características de raça, linhagem, faixa etária e sexo. Aos 30 dias de idade, todos os animais foram submetidos a uma triagem com a aplicação de tuberculina PPD bovina, pela via intradérmica na base da orelha e não houve qualquer tipo de reação. Decorridos 60 dias do teste tuberculínico de triagem, o grupo A recebeu injeção intramuscular de 0,5 mL de uma suspensão oleosa de M. avium estirpe D4; o grupo B recebeu 0,5 mL de uma suspensão oleosa de M. bovis estirpe AN5; o grupo C (controle I), recebeu 0,5 mL do adjuvante oleoso; e o grupo D (controle II), recebeu 0,5 mL de solução fisiológica. Após 30 dias da sensibilização foi realizada a prova de tuberculinização comparativa com reação medida pela variação da espessura da pele com cutímetro de mola às 0h, 24h, 48h e 72h, após a aplicação das tuberculinas. No teste comparativo, lido às 48 ou 72 horas, a reação foi considerada negativa quando a diferença das reações entre o PPD bovino e o PPD aviário foi menor que 6,7 mm; suspeito ou inconclusivo quando a diferença se situou na faixa de 6,7 a 7,5 mm; e positiva de acordo com o tipo de PPD, considerando-se tuberculose para PPD M. bovis e micobacteriose para PPD M. avium, quando a diferença da reação foi superior a 7,5 mm.(AU)
The diagnostic value of the cutaneous allergic response to tuberculin in piglets experimentally sensitized intramuscularly with the oily suspensions of heat inactivated M. bovis or M. avium was investigated. Ninety-one animals were used and divided into four groups: groups A and B were formed each with 25 individuals, and groups C and D, with 21 and 20 individuals, respectively, balancing the characteristics of race, ancestry, age and sex. At the age of 30 days, all the animals were submitted to the screening test with the use of M. bovis PPD, by the intradermal route at the base of the ear and no reaction was detected. Sixty days after the screening tuberculin test, animals of the group A were injected intramuscularly with 0.5 mL of oily suspension of M. avium D4 strain; animals of the group B received 0.5 mL of an oily suspension of M. bovis, AN5 strain; group C (control I) received 0.5 mL of an oily adjuvant; and the individuals of the group D (control II) received 0.5 mL of saline solution. Following 30 days of sensitization, comparative skin reactions were measured by the variation in skin thickness with a caliper at 0h, 24h, 48h an 72h after applications of tuberculins. In the comparative test measured at 48 or 72h, the reaction was considered negative when the difference of the reactions between bovine PPD and avian PPD was less than 6.7 mm; suspected or inconclusive, when the difference stood in the range of 6.7 to 7.5 mm; and positive according to the type of PPD, considering tuberculosis the M. bovis PPD and mycobacteriosis the M. avium PPD, when the difference of the reaction was greater than 7.5 mm.(AU)
Assuntos
Animais , Suínos , Tuberculose/diagnóstico , Infecções por Mycobacterium/diagnóstico , Teste Tuberculínico/veterinária , Mycobacterium bovis/isolamento & purificação , Mycobacterium avium/isolamento & purificação , Doenças dos Suínos/diagnóstico , Tuberculose/veterinária , Infecções por Mycobacterium/veterinária , Teste Tuberculínico/métodosResumo
The aim of this work was to isolate and characterize microorganisms in hypertrophied lymph nodes or gross lesions suggestive of tuberculosis collected from 12 goats and 28 sheep slaughtered at the public slaughterhouse of Patos municipality, Paraíba State, in the Northeast region of Brazil. The identification of mycobacteria was performed by the PRA method (PCR-Restriction Enzyme Analysis). Histopathological examination of lesions was also performed. Organs affected were liver, lung, mammary gland, bladder and mediastinal, mesenteric, submandibular, parotid, popliteal, precrural, prescapular and superficial inguinal lymph nodes. Histopathological examination showed the presence of granulomas in 8 (20.00%) animals. Of the 12 goats, 1 (8.33%) was positive in the culture of mycobacteria, and by PRA method the isolate was classified as belonging to the M. tuberculosis complex. Two (7.14%) sheep were positive for the presence of environmental mycobacteria. There was isolation of Corynebacterium pseudotuberculosis in 8 (66.66%) goats and 17 (60.71%) sheep, and simultaneous isolation of mycobacteria and C. pseudotuberculosis in 1 (8.33%) goat and 1 (3.57%) sheep. The isolation of mycobacteria of the M. tuberculosis complex in goats in this study raises concerns of public health, as professionals involved in handling these animals and the meat and milk consumers are exposed to the risk [...]
O objetivo do presente trabalho foi isolar e tipificar micro-organismos presentes em linfonodos hipertrofiados ou lesões macroscópicas sugestivas de tuberculose colhidos de 12 caprinos e 28 ovinos abatidos no matadouro público do município de Patos, Paraíba. A identificação de micobactérias foi feita com o método PRA (PCR-Restriction Enzyme Analysis). Também foi realizado o exame histopatológico das lesões. Os órgãos afetados foram fígado, pulmão, glândula mamária, bexiga e linfonodos mediastínicos, mesentéricos, submandibulares, parotídeos, poplíteos, pré-crural, pré-escapular e inguinal superficial. O exame histopatológico apontou a presença de granulomas em 8 (20,00%) animais. Dos 12 caprinos, 1 (8,33%) foi positivo no cultivo de micobactérias, e pelo método PRA o isolado foi classificado como pertencente ao complexo M. tuberculosis. Dois (7,14%) ovinos foram positivos para a presença de micobactérias ambientais. Houve isolamento de Corynebacterium pseudotuberculosis em 8 (66,66%) caprinos e em 17 (60,71%) ovinos, e isolamento simultâneo de micobactérias e C. pseudotuberculosis em 1 (8,33%) caprino e 1 (3,57%) ovino. O isolamento de micobactéria do complexo M. tuberculosis em caprinos no presente trabalho levanta preocupações do ponto de vista de saúde pública, uma vez que profissionais envolvidos na manipulação destes animais, bem como a população consumidora de carne [...]
Assuntos
Animais , Inocuidade dos Alimentos , Mycobacterium tuberculosis , Ovinos , Ruminantes , Linfonodos , Saneamento de MercadosResumo
The aim of this work was to isolate and characterize microorganisms in hypertrophied lymph nodes or gross lesions suggestive of tuberculosis collected from 12 goats and 28 sheep slaughtered at the public slaughterhouse of Patos municipality, Paraíba State, in the Northeast region of Brazil. The identification of mycobacteria was performed by the PRA method (PCR-Restriction Enzyme Analysis). Histopathological examination of lesions was also performed. Organs affected were liver, lung, mammary gland, bladder and mediastinal, mesenteric, submandibular, parotid, popliteal, precrural, prescapular and superficial inguinal lymph nodes. Histopathological examination showed the presence of granulomas in 8 (20.00%) animals. Of the 12 goats, 1 (8.33%) was positive in the culture of mycobacteria, and by PRA method the isolate was classified as belonging to the M. tuberculosis complex. Two (7.14%) sheep were positive for the presence of environmental mycobacteria. There was isolation of Corynebacterium pseudotuberculosis in 8 (66.66%) goats and 17 (60.71%) sheep, and simultaneous isolation of mycobacteria and C. pseudotuberculosis in 1 (8.33%) goat and 1 (3.57%) sheep. The isolation of mycobacteria of the M. tuberculosis complex in goats in this study raises concerns of public health, as professionals involved in handling these animals and the meat and milk consumers are exposed to the risk [...](AU)
O objetivo do presente trabalho foi isolar e tipificar micro-organismos presentes em linfonodos hipertrofiados ou lesões macroscópicas sugestivas de tuberculose colhidos de 12 caprinos e 28 ovinos abatidos no matadouro público do município de Patos, Paraíba. A identificação de micobactérias foi feita com o método PRA (PCR-Restriction Enzyme Analysis). Também foi realizado o exame histopatológico das lesões. Os órgãos afetados foram fígado, pulmão, glândula mamária, bexiga e linfonodos mediastínicos, mesentéricos, submandibulares, parotídeos, poplíteos, pré-crural, pré-escapular e inguinal superficial. O exame histopatológico apontou a presença de granulomas em 8 (20,00%) animais. Dos 12 caprinos, 1 (8,33%) foi positivo no cultivo de micobactérias, e pelo método PRA o isolado foi classificado como pertencente ao complexo M. tuberculosis. Dois (7,14%) ovinos foram positivos para a presença de micobactérias ambientais. Houve isolamento de Corynebacterium pseudotuberculosis em 8 (66,66%) caprinos e em 17 (60,71%) ovinos, e isolamento simultâneo de micobactérias e C. pseudotuberculosis em 1 (8,33%) caprino e 1 (3,57%) ovino. O isolamento de micobactéria do complexo M. tuberculosis em caprinos no presente trabalho levanta preocupações do ponto de vista de saúde pública, uma vez que profissionais envolvidos na manipulação destes animais, bem como a população consumidora de carne [...](AU)
Assuntos
Animais , Mycobacterium tuberculosis , Ovinos , Ruminantes , Inocuidade dos Alimentos , Saneamento de Mercados , LinfonodosResumo
The aim of this work was to isolate and characterize microorganisms in hypertrophied lymph nodes or gross lesions suggestive of tuberculosis collected from 12 goats and 28 sheep slaughtered at the public slaughterhouse of Patos municipality, Paraíba State, in the Northeast region of Brazil. The identification of mycobacteria was performed by the PRA method (PCR-Restriction Enzyme Analysis). Histopathological examination of lesions was also performed. Organs affected were liver, lung, mammary gland, bladder and mediastinal, mesenteric, submandibular, parotid, popliteal, precrural, prescapular and superficial inguinal lymph nodes. Histopathological examination showed the presence of granulomas in 8 (20.00%) animals. Of the 12 goats, 1 (8.33%) was positive in the culture of mycobacteria, and by PRA method the isolate was classified as belonging to the M. tuberculosis complex. Two (7.14%) sheep were positive for the presence of environmental mycobacteria. There was isolation of Corynebacterium pseudotuberculosis in 8 (66.66%) goats and 17 (60.71%) sheep, and simultaneous isolation of mycobacteria and C. pseudotuberculosis in 1 (8.33%) goat and 1 (3.57%) sheep. The isolation of mycobacteria of the M. tuberculosis complex in goats in this study raises concerns of public health, as professionals involved in handling these animals and the meat and milk consumers are exposed to the risk of infection.
O objetivo do presente trabalho foi isolar e tipificar micro-organismos presentes em linfonodos hipertrofiados ou lesões macroscópicas sugestivas de tuberculose colhidos de 12 caprinos e 28 ovinos abatidos no matadouro público do município de Patos, Paraíba. A identificação de micobactérias foi feita com o método PRA (PCR-Restriction Enzyme Analysis). Também foi realizado o exame histopatológico das lesões. Os órgãos afetados foram fígado, pulmão, glândula mamária, bexiga e linfonodos mediastínicos, mesentéricos, submandibulares, parotídeos, poplíteos, pré-crural, pré-escapular e inguinal superficial. O exame histopatológico apontou a presença de granulomas em 8 (20,00%) animais. Dos 12 caprinos, 1 (8,33%) foi positivo no cultivo de micobactérias, e pelo método PRA o isolado foi classificado como pertencente ao complexo M. tuberculosis. Dois (7,14%) ovinos foram positivos para a presença de micobactérias ambientais. Houve isolamento de Corynebacterium pseudotuberculosis em 8 (66,66%) caprinos e em 17 (60,71%) ovinos, e isolamento simultâneo de micobactérias e C. pseudotuberculosis em 1 (8,33%) caprino e 1 (3,57%) ovino. O isolamento de micobactéria do complexo M. tuberculosis em caprinos no presente trabalho levanta preocupações do ponto de vista de saúde pública, uma vez que profissionais envolvidos na manipulação destes animais, bem como a população consumidora de carne e leite, estão expostos ao risco de infecção.
Resumo
As micobacterioses afetam várias espécies de peixes, tanto comerciais como ornamentais. É uma enfermidade sistêmica com formação de granulomas, conhecida como micobacteriose de peixes. O desenvolvimento da aquicultura tem aumentado o número de casos com essa enfermidade que muitas vezes é de difícil diagnóstico. Neste trabalho apresentamos dois casos de micobacterioses em Paralichthys orbignyanus e Elacatinus figaro provenientes do Laboratório de Piscicultura Estuarina e Marinha da Universidade Federal do Rio Grande (FURG). As micobactérias de peixes possuem menos ácidos graxos que as de mamíferos, por isso foi utilizado o protocolo de coloração de Fite-Faraco, uma vez que o protocolo de coloração clássico de Ziehl Neelsen pode resultar em falsos negativos. Para determinar o tipo de micobactéria foi utilizada a imuno-histoquímica com a qual se pode diagnosticar a presença de Mycobacterium marinum. A enfermidade pode ser transmitida para o homem e com esse trabalho ressalta-se a necessidade de fazer o diagnóstico correto e implantar medidas de proteção para as pessoas que estão em contato com os peixes infectados.(AU)
Mycobacteriosis affects various species of fish, including those cultured with commercial purposes. It is a chronic systemic with granuloma forming disease known as fish mycobacteriosis. In the last few years the interest of fish culture has been increasing due to the reduction of the natural fish stocks. However the intensification of fish culture has collaborated to the development of diseases such as mycobacteriosis, causing losses due to the chronic characteristics and difficult diagnosis. This study is to report two cases of mycobacteriosis observed at "Laboratório de Piscicultura Estuarina e Marinha of the Universidade Federal do Rio Grande (FURG)", one for Brazilian flounder Paralichthys orbignyanus and another for barber goby Elacatinus figaro. The Fite-Faraco staining protocol was used. Membranes of mycobacterial cells contain a waxy substance composed of mycolic acids. These are ß-hydroxy carboxylic acids with chain lengths of up to 90 carbon atoms. The property of acid fastness is related to the carbon chain length of the mycolic acid found in any particular species. The fish mycobacterium is much less acid and alcohol fast than the tubercle bacillus and the classic Ziehl-Neelsen Staining Protocol may give false results. The presence of mycobacterial antigen lesions was studied by immunohistological methods using monoclonal antibodies to Mycobacterium marinum. Humans may be infected by these bacteria and develop cutaneous granuloma. In this paper the necessity is stressed for a correct diagnosis of the disease, contributing to the prevention of its establishment in aquatic facilities, and thus, preserving cultured fish as well as human beings from infection.(AU)
Assuntos
Animais , Doenças dos Peixes/diagnóstico , Linguado/microbiologia , Pesqueiros/prevenção & controle , Infecções por Mycobacterium/veterinária , Intestinos/microbiologia , Sinais e Sintomas/veterinária , Síndrome de Resposta Inflamatória Sistêmica/veterinária , Coloração e Rotulagem/veterináriaResumo
Background: Cutaneous mycobacterioses show a worldwide distribution and manifest as three forms: atypical mycobacteriosis, feline leprosy and cutaneous tuberculosis. Atypical cutaneous mycobacteriosis in cats is a rare disease commonly caused by fast-growing mycobacteria belonging group IV of the Runyon classification. This report describes for the first time an autochthonous case of atypical cutaneous mycobacteriosis in a cat from the municipality of Rio de Janeiro. Case: A castrated female mongrel cat presented multiple ulcerated, exudative and alopecic lesions in different regions of the body. After sedation of the animal with 1% acepromazine hydrochloride (0.1 mg/kg) and 10% ketamine hydrochloride (10 mg/kg), clinical examination was performed and an exudate was collected from the ulcerated skin lesion for cytopathological analysis and mycological culture. The slide containing the lesion impression was stained by a rapid panoptic method. For fungal culture, material was collected with a sterile swab, seeded onto Sabouraud dextrose agar containing chloramphenicol and mycobiotic agar, and incubated at 25ºC. After antisepsis and asepsis, local anesthesia was performed with 2% lidocaine hydrochloride without vasoconstrictor and a 4 mm punch biopsy was collected from the lesion, fixed in 10% buffered formalin and sent for histopathological analysis. No fungal structures were detected by cytopathological or mycological analysis. Histopathological examination revealed ulcerated skin and the presence of a marked and diffuse pyogranulomatous infiltrate in the superficial and deep dermis, which also involving the subcutaneous tissue and underlying muscle tissue. Grocotts or PAS staining did not identify fungal structures. The Wade method was positive for acid-fast bacteria suggestive of Mycobacterium. Analysis of the hematological and biochemical profiles revealed no noteworthy alterations.(...)
Assuntos
Feminino , Animais , Gatos , Infecções por Mycobacterium/diagnóstico , Infecções por Mycobacterium/veterinária , MycobacteriumResumo
Background: Cutaneous mycobacterioses show a worldwide distribution and manifest as three forms: atypical mycobacteriosis, feline leprosy and cutaneous tuberculosis. Atypical cutaneous mycobacteriosis in cats is a rare disease commonly caused by fast-growing mycobacteria belonging group IV of the Runyon classification. This report describes for the first time an autochthonous case of atypical cutaneous mycobacteriosis in a cat from the municipality of Rio de Janeiro. Case: A castrated female mongrel cat presented multiple ulcerated, exudative and alopecic lesions in different regions of the body. After sedation of the animal with 1% acepromazine hydrochloride (0.1 mg/kg) and 10% ketamine hydrochloride (10 mg/kg), clinical examination was performed and an exudate was collected from the ulcerated skin lesion for cytopathological analysis and mycological culture. The slide containing the lesion impression was stained by a rapid panoptic method. For fungal culture, material was collected with a sterile swab, seeded onto Sabouraud dextrose agar containing chloramphenicol and mycobiotic agar, and incubated at 25ºC. After antisepsis and asepsis, local anesthesia was performed with 2% lidocaine hydrochloride without vasoconstrictor and a 4 mm punch biopsy was collected from the lesion, fixed in 10% buffered formalin and sent for histopathological analysis. No fungal structures were detected by cytopathological or mycological analysis. Histopathological examination revealed ulcerated skin and the presence of a marked and diffuse pyogranulomatous infiltrate in the superficial and deep dermis, which also involving the subcutaneous tissue and underlying muscle tissue. Grocotts or PAS staining did not identify fungal structures. The Wade method was positive for acid-fast bacteria suggestive of Mycobacterium. Analysis of the hematological and biochemical profiles revealed no noteworthy alterations.(...)(AU)
Assuntos
Animais , Feminino , Gatos , Infecções por Mycobacterium/diagnóstico , Infecções por Mycobacterium/veterinária , Mycobacterium , /tratamento farmacológicoResumo
O gênero Mycobacterium compreende inúmeras espécies estudadas por sua capacidade de causar doenças. Surtos de tuberculose e micobacteriose em zoológicos, aquários, centros de pesquisa e criadouros evidenciaram a importância desses organismos em relação à saúde de animais silvestres mantidos em cativeiro. Duzentas e onze amostras, de 101 animais entre mamíferos, aves, répteis, anfíbios e peixes, foram submetidas à cultura. Foram isolados M. tuberculosis, M. bovis e 13 espécies de MNT associadas à doença. A natureza crônica dessas doenças, seu diagnóstico ante mortem limitado, juntamente com as características de interação social de cada espécie e o confinamento, favorecem a transmissão das micobactérias pelo contato com humanos ou outros animais infectados. Apesar de a presença de M. tuberculosis e M. bovis em instituições brasileiras já ter sido constatada, o presente estudo descreveu infecções em espécies animais utilizadas para consumo humano, e em espécies conhecidas pela suscetibilidade, mas que ainda não foram documentadas no Brasil. Doença causada por MNT foi relatada, mas sua importância deve ser considerada de acordo com a espécie animal acometida.
Mycobacterium genus comprises several species, which have been studied for their capacity to cause diseases. Outbreaks of tuberculosis and mycobacteriosis in zoos, aquariums, research and breeding facilities have shown the importance of these organisms to health of wildlife animal maintained in captivity. Two hundred and eleven samples, from 101 animals among mammal, birds, reptiles, amphibians and fishes, were cultured. Mycobacterium tuberculosis, M. bovis and 13 species of nontuberculous mycobacteria associated to diseases were isolated. The chronical nature of tuberculosis and mycobacteriosis, their limited ante mortem diagnosis, along with social behavior of each species and confinement, favor mycobacterial transmission through contact with infected humans and other animals. Despite the presence of M. tuberculosis and M. bovis has already been observed in Brazilian institutions, the present study described infections in animal species destined to human consumption, and species known for their susceptiblility, yet not documented in Brazil. Disease caused by non-tuberculous mycobacteria was reported, though its importance must be considered according to the animal species affected.
Resumo
Foi investigado o valor diagnóstico da resposta alérgica cutânea à tuberculina e do ELISA indireto, com antígeno recombinante MPB 70, em leitões experimentalmente sensibilizados, pela via intramuscular, com suspensões oleosas de M. bovis ou M. avium inativados. Foram utilizados 91 animais divididos em quatro grupos. Os grupos A e B, cada um com 25 indivíduos, grupos C e D com 21 e 20 indivíduos respectivamente, balanceando-se as características de raça, linhagem, faixa etária e sexo. [...] Nos exames histopatológicos, foi observado intenso infiltrado inflamatório linfocitário no local das reações intradérmicas dos animais testados com o PPD homologo ao tipo de micobactéria utilizada na suspensão oleosa sensibilizante. O ensaio de ELISA com antígeno, MPB 70 recombinante, foi capaz de revelar a presença de anticorpos contra o M. bovis, porém não revelou anticorpos para M. avium
The diagnostic value of the cutaneous allergic response to tuberculin and Indirect ELISA test was investigated using MPB 70 recombinant antigen, in piglets experimentally sensitized intramuscularly with the oily suspensions of inactivated M. bovis or M. avium. The ninety-one animals used were divided into four groups. The groups A and B were formed each with 25 individuals, and groups C and D, with 21 and 20 individuals, respectively, balancing the characteristics of race, ancestry, age and sex. [...] In histopathological examinations, intense lymphocytic inflammatory infiltrate were observed at the site of intradermal reactions of the animals tested with PPD homologous to the type of mycobacteria used in sensitizing oil suspension. The ELISA assay with MPB 70 recombinant antigen was able to reveal the presence of antibodies against M. bovis, but did not reveal antibodies to M. avium
Assuntos
Animais , Doenças dos Suínos/patologia , Infecções por Mycobacterium/diagnóstico , Infecções por Mycobacterium/veterinária , Tuberculose/diagnóstico , Tuberculose/veterinária , Proteínas Recombinantes/imunologia , Testes Cutâneos/veterináriaResumo
Foi investigado o valor diagnóstico da resposta alérgica cutânea à tuberculina e do ELISA indireto, com antígeno recombinante MPB 70, em leitões experimentalmente sensibilizados, pela via intramuscular, com suspensões oleosas de M. bovis ou M. avium inativados. Foram utilizados 91 animais divididos em quatro grupos. Os grupos A e B, cada um com 25 indivíduos, grupos C e D com 21 e 20 indivíduos respectivamente, balanceando-se as características de raça, linhagem, faixa etária e sexo. [...] Nos exames histopatológicos, foi observado intenso infiltrado inflamatório linfocitário no local das reações intradérmicas dos animais testados com o PPD homologo ao tipo de micobactéria utilizada na suspensão oleosa sensibilizante. O ensaio de ELISA com antígeno, MPB 70 recombinante, foi capaz de revelar a presença de anticorpos contra o M. bovis, porém não revelou anticorpos para M. avium (AU)
The diagnostic value of the cutaneous allergic response to tuberculin and Indirect ELISA test was investigated using MPB 70 recombinant antigen, in piglets experimentally sensitized intramuscularly with the oily suspensions of inactivated M. bovis or M. avium. The ninety-one animals used were divided into four groups. The groups A and B were formed each with 25 individuals, and groups C and D, with 21 and 20 individuals, respectively, balancing the characteristics of race, ancestry, age and sex. [...] In histopathological examinations, intense lymphocytic inflammatory infiltrate were observed at the site of intradermal reactions of the animals tested with PPD homologous to the type of mycobacteria used in sensitizing oil suspension. The ELISA assay with MPB 70 recombinant antigen was able to reveal the presence of antibodies against M. bovis, but did not reveal antibodies to M. avium (AU)
Assuntos
Animais , Doenças dos Suínos/patologia , Tuberculose/diagnóstico , Tuberculose/veterinária , Infecções por Mycobacterium/diagnóstico , Infecções por Mycobacterium/veterinária , Testes Cutâneos/veterinária , Proteínas Recombinantes/imunologiaResumo
Relata-se, pela primeira vez no Brasil, um caso de dermatite decorrente de infecção por micobactéria atípica do complexo Mycobacterium fortuitum-peregrinum, em espécie felina, sem raça definida, fêmea, com cinco anos de idade. Há oito meses, evoluía com lesões maculares equimóticas, nodulares, erosadas, ulceradas, acompanhadas de fístulas exsudativas, com intenso prurido e algia. Evidenciou-se a presença de micobactéria do complexo Mycobacterium fortuitum-peregrinum (grupo IV de Runyon ) identificada após evidenciação histopatológica, cultivo bacteriano e por testes bioquímicos. Após dois meses de terapia sistêmica com enrofloxacina (5mg/kg/Bid/VO) e tópica (triclosan e rifamicina) houve involução das lesões, com efeitos colaterais discretos.(AU)
A case of dermatitis caused by atypical mycobateria of Mycobacterium fortuitum peregrinum complex was observed for the first time in Brazil. A five-year-old female mixed breed cat had a dermatitis process which had started eight months before. Lesions were characterized by macules, nodules, erosions, ulcers and exsudative fistulas, with intensive pruritus and pain. It had failed to respond to clinical treatment and surgical excision. The diagnosis was based on history, physical and dermatologic examination and complementary tests (cytologic, bacterioscopic, bacterial culture, histopathological, hemathological, ultrasonographic, radiographic and electrocardiographic). The presence of mycobacteria of Mycobacterium fortuitum-peregrinum complex (group IV of Runyon) was observed and identified after histopathological evidence, bacterial culture and biochemical tests. After two months of systemic therapy with enrofloxacin (5mg/kg, orally/twice a day) and topic treatment with triclosan and rifamicin, rapid improvement of the clinical appearance of the lesions were observed with low incidence of side-effects.(AU)
Assuntos
Animais , Dermatite/diagnóstico , Mycobacterium fortuitum/isolamento & purificação , Infecções por Mycobacterium não Tuberculosas/diagnóstico , Infecções por Mycobacterium não Tuberculosas/epidemiologia , GatosResumo
In veterinary dermatology, domestic carnivores sporadically are found with ulcerated lesions characterized by a prolonged course of evolution and by relative unresponsiveness to the usual drug therapy; this condition can be due to infections caused by microorganisms of the Genus Mycobacterium. Due to the scarcity of its description in Brazilian literature, and due to the inusitate lesions and response to preconized therapy, two cases of canine mycobacteriosis attended at the University Veterinary Teaching Hospital of the FMVZ/USP are then described. The adult bitches (German Shepherd and Doberman Pinscher) were found with ulcerated lesions at the posterior side of the pinna w ith granulomatous aspect and with exudation and covered by hemorrhagic crusts without showing any detectable satellite lymphadenomegalia or any symptoms in other systemic organs. Physical, hematologic and radiographic examinations were performed and lesions have been curetted and biopsied through punching technique, and specimens were examined histologically using Holst-Mitchinson and Radhakrishna and Ziehl-Neelsen staining, and throught culture methods using Lowenstein-Jensen and Stonebrink & Leslie media at different temperature of incubation. Intradermal tuberculin test using PPD tuberculin of mammalian and avian origin were performed. Presence of alcohol-acid resistant bacilli were detected through
Na clínica dermatológica veterinária por vezes atendem-se carnívoros domésticos com lesões ulceradas de evolução prolongada e, geralmente, rebeldes à terapia usual, que podem ser originárias de infecções por micobactérias do gênero Mycobacterium. Pela escassez de descrições na bibliografia brasileira, pelo inusitado das lesões e pela resposta à terapia preconizada, descrevem-se dois casos de micobacteriose em caninos atendidos na FMVZ/USP. As cadelas (Pastor Alemão, Doberman), adultas, apresentavam lesões ulceradas graves, nas faces posteriores dos pavilhões auriculares, de aspecto granulomatoso, exsudativas, recobertas por crostas hemorrágicas. Inexistiam linfoadenomegalia satélite e tampouco sintomas nos demais sistemas orgânicos. Complementaram-se os exames físico, hematológico e radiológico por curetagem das lesões e biópsias de pele que foram então submetidas a exames histopatológico, bacteriológico (direto: técnicas de Holst-Mitchinson e Radhakrishna, Ziehl-Neelsen); cultivo (meios de Lowenstein-Jensen e de Stonebrink & Leslie), em diferentes temperaturas; teste de tuberculina (PPD mamífero e aviário). Demonstrou-se, na bacterioscopia e na histopatologia, a presença de bacilos ácido-álcool resistentes que não cresceram nos meios de cultivo convencionais, mesmo quando submetidos a várias temperaturas distintas, bem como quadro tecidual piogranulomatoso típico. Após a i
Resumo
Background: : : Cutaneous mycobacterioses show a worldwide distribution and manifest as three forms: atypical mycobacteriosis, feline leprosy and cutaneous tuberculosis. Atypical cutaneous mycobacteriosis in cats is a rare disease commonly caused by fast-growing mycobacteria belonging group IV of the Runyon classification. This report describes for the first time an autochthonous case of atypical cutaneous mycobacteriosis in a cat from the municipality of Rio de Janeiro. Case: A castrated female mongrel cat presented multiple ulcerated, exudative and alopecic lesions in different regions of the body. After sedation of the animal with 1% acepromazine hydrochloride (0.1 mg/kg) and 10% ketamine hydrochloride (10 mg/kg), clinical examination was performed and an exudate was collected from the ulcerated skin lesion for cytopathological analysis and mycological culture. The slide containing the lesion impression was stained by a rapid panoptic method. For fungal culture, material was collected with a sterile swab, seeded onto Sabouraud dextrose agar containing chloramphenicol and mycobiotic agar, and incubated at 25ºC. After antisepsis and asepsis, local anesthesia was performed with 2% lidocaine hydrochloride without vasoconstrictor and a 4 mm punch biopsy was collected from the lesion, fixed in 10% buffered formalin and sent for histopathological analysis. No fungal structures we
Resumo
Background: : : Cutaneous mycobacterioses show a worldwide distribution and manifest as three forms: atypical mycobacteriosis, feline leprosy and cutaneous tuberculosis. Atypical cutaneous mycobacteriosis in cats is a rare disease commonly caused by fast-growing mycobacteria belonging group IV of the Runyon classification. This report describes for the first time an autochthonous case of atypical cutaneous mycobacteriosis in a cat from the municipality of Rio de Janeiro. Case: A castrated female mongrel cat presented multiple ulcerated, exudative and alopecic lesions in different regions of the body. After sedation of the animal with 1% acepromazine hydrochloride (0.1 mg/kg) and 10% ketamine hydrochloride (10 mg/kg), clinical examination was performed and an exudate was collected from the ulcerated skin lesion for cytopathological analysis and mycological culture. The slide containing the lesion impression was stained by a rapid panoptic method. For fungal culture, material was collected with a sterile swab, seeded onto Sabouraud dextrose agar containing chloramphenicol and mycobiotic agar, and incubated at 25ºC. After antisepsis and asepsis, local anesthesia was performed with 2% lidocaine hydrochloride without vasoconstrictor and a 4 mm punch biopsy was collected from the lesion, fixed in 10% buffered formalin and sent for histopathological analysis. No fungal structures we
Resumo
The finding of four clusters of M. avium (PIG-A, B, C and D), typed by the IS1245-RFLP method, infecting the swine population of the south region of Brazil, the possible existence of virulence differences among them, the role of the virulence in the transmission mechanisms of infections and the existence of reasonable doubts regarding the importance of horizontal transmission for swine micobacteriosis, the virulence of these four strains of M. avium were compared. Bacteria from each cluster were inoculated in 48 hamsters by intra-peritoneal route. On the 2nd, 13th, 26th, and 40th days after inoculation, (T1 to T4), 12 animals of each cluster were sacrificed with vapors of ethyl ether and the bacteria were quantified in the liver, spleen and lung. Results were expressed as cfu/g of organ. The presence of the strains was verified in the blood and histological exams were also accomplished. The four strains induced granulomatous lesions in the liver and spleen since 2 days after inoculation and were disseminated to the lungs through the blood stream. The cfu counts from spleen were always bigger them that obtained from liver and lungs. Differences among strains were observed through the analysis of cfu counts from spleen (T1: p; PIG-A>; PIG-D>; PIG-C.
Tendo sido comprovada a existência de quatro famílias molecularmente distintas de M. avium (PIG-A, B, C e D) circulando em suínos da região sul do Brasil, e havendo dúvidas a respeito da importância da transmissão horizontal como mecanismo de manutenção da doença, o presente teve por objetivo estudar a virulência dessas estirpes, informação importante para o aperfeiçoamento dos métodos de controle. Uma estirpe representante de cada família foi inoculada pela via intra-peritoneal em 48 hamsters com uma dose de 30.000 U.F.C. por animal. Após 2, 13, 26 e 40 dias da inoculação (T1 a T4), 12 hamsters inoculados de cada família foram anestesiados, sacrificados e os agentes foram quantificados no fígado, baço e pulmão. Os resultados foram expressos em número de U.F.C./g de órgão. A presença das estirpes foi pesquisada no sangue e também foram realizados exames histológicos. As estirpes PIG-A, B, C e D induziram a formação de lesões granulomatosas no fígado e baço a partir do segundo dia pós-inoculação e disseminaram-se pela via hemática, alcançando os pulmões. O baço sempre apresentou maiores contagens de U.F.C., seguido pelo figado e pulmões. Diferenças entre as estirpes foram constatadas através de análises das contagens de U.F.C de baço (T1: p; PIG-A>; PIG-D>; PIG-C.
Resumo
Macroscopic lesions suggestive of tuberculosis in swine lymph nodes caused by mycobacteria were already described in many countries, including Brazil. This survey was performed in a farm with previous diagnostic of tuberculosis: lymph nodes were obtained from 146 pigs, aged between 2.5 and >; 14 months, and after macroscopic and microscopic examination they were cultured. Furthermore feed, water and sawdust were cultured. Macroscopic lesions were characterized as nodes with diameter between 1-2 mm, with a pale yellow, loose, caseous content. Lymph nodes from 12 pigs were positive for acid-fast bacteria. Mycobacterium-group III (Runyon) was isolated in four cases. The lesions occurred mainly in pigs aged between 6 and 7 months old. The same mycobacteria were isolated from sawdust. It is concluded that sawdust might be the source of infection.
Condenações de suínos no momento do abate devido a lesões tuberculóides causadas por micobactérias são descritas em diversos países, inclusive no Brasil. No presente trabalho, 146 animais de idades variando entre 2,5 e >; 14 meses, provenientes de uma propriedade com problema de condenações por tuberculose foram abatidos e os linfonodos examinados quanto ao tipo de lesão macro e microscópica e à presença de micobactérias. Ao mesmo tempo, a ração, a água e a maravalha utilizadas na propriedade foram investigadas quanto à presença de micobactérias. Macroscopicamente, os linfonodos afetados apresentavam nódulos arredondados de 1-2 mm de diâmetro, de coloração amarelo-clara com conteúdo pastoso a caseoso, delimitados por uma cápsula. Em linfonodos de 12 animais foi possível visualizar bacilos álcool-ácido resistentes, sendo que em 4 deles foi isolado o Mycobacterium-grupo III (Runyon). As lesões foram encontradas principalmente em animais entre 6 e 14 meses de idade. A mesma bactéria foi isolada da maravalha, que pode ter sido a fonte de infecção dos animais.
Resumo
A escassez de dados sobre tuberculose e micobacterioses em ovinos (Ovis aries) motivou o presente trabalho de isolamento e tipificação de microorganismos presentes em linfonodos e lesões macroscópicas sugestivas de tuberculose. Foram avaliados pelo teste tuberculinico, 353 ovinos das raças Santa Inês e Texel de duas propriedades da região de Pindamonhangaba - São Paulo. Dos 57 animais selecionados para abate, 31 apresentavam reação ao PPD bovino maior que ao PPD aviário e 26 com reação ao PPD bovino menor que ao PPD aviário. Onze animais (19,3%) apresentaram na necrópsia lesões sugestivas de tuberculose. Os órgãos afetados foram o fígado, linfonodo submandibular, intestino, pulmão, linfonodo mediastino e glândula mamária. Foram isoladas micobactérias de sete (12,3%) animais e a tipificação genética pelo método de PRA demonstrou cinco (71,42%) infectados pelo Mycobacterium flavescens 1, um (14,28%) pelo M. kansasi, e um (14,28%) por micobactéria pertencente ao Complexo M. tuberculosis. Exames bacteriológicos para outras bactérias e/ou fungos isolaram Corynebacterium pseudotuberculosis em quatro (7,01%) dos 57 animais abatidos. Houve isolamento simultâneo de micobactérias e de Corynebacterium pseudotuberculosis em dois (3,5%) dos 57 animais abatidos. Os exames histopatológicos apontaram em nove (15,78%) animais a presença de granuloma e coloração de Ziehl-Neelsen positivo. A análise dos resultados obtidos permitiram concluir que, neste trabalho, os testes imunoalérgicos (Teste Cervical Simples e Teste Cervical Comparativo) não foram capazes de diferenciar infecção provocada pelo M. flavescens 1, M. kansasi, complexo M. tuberculosis e C. pseudotuberculosis. Nos exames macroscópico e histopatológico lesões provocadas por M. flavescens 1, M. kansasi, e C. pseudotuberculosis não foram diferenciáveis das provocadas pelo complexo M. tuberculosis
The occurrence of few data on ovine (Ovis aries) tuberculosis and mycobacteriosis has motivated this work of isolation and typing microorganism found in lymph nodes and tuberculosis-like gross lesions. Tuberculin skin test was performed in 353 Santa Ines and Texel ovine breeds of two properties located at Pindamonhangaba Municipality - Sao Paulo State. Fifty seven animals were selected to be slaughtered and 31 of them had the bovine PPD skin test higher than avian PPD and other 26 presented bovine PPD reaction lower than avian PPD. Eleven animals (19.3%) showed tuberculosis-like gross lesions at necropsy. Most affected organs were liver, submandibular lymph nodes, intestines, lungs, mediastinic lymph nodes and mammary gland. It was possible to isolate mycobacteria from seven (12.3%) animals and genetic typing by the PRA method showed that five animals (71.42%) were infected with Mycobacterium flavescens 1, one (14.28%) with M. kansasi, and one (14.28%) with M. tuberculosis complex mycobacteria. Bacteriological culture isolation for other bacteria and/or fungi were positive for Corynebacterium pseudotuberculosis in four (7.01%) of 57 slaughtered animals. There was a concomitant isolation of mycobacteria and Corynebacterium pseudotuberculosis in two (3.5%) of 57 slaughtered animals. Histopathologic examination demonstrated the presence of granuloma and positive Ziehl-Neelsen staining in nine (15.78%) animals. Results analysis allowed concluding that in this work, immuno-allergic tests (Simple Cervical Test and Comparative Cervical Test) were not capable to differentiate infection caused by M. flavescens 1, M. kansasi, M. tuberculosis complex and C. pseudotuberculosis. During gross examination and histopathology, lesions caused by M. flavescens 1, M. kansasi, and C. pseudotuberculosis were not distinguishable from those caused by M. tuberculosis complex
Resumo
Tendo sido comprovada a existência da famílias molecularmente distintas de M. avium circulando em suínos de região sul do Brasil, e havendo dúvidas a respeito da importância da transmissão horizontal como mecanismo de manutenção da doença, o presente teve por objetivo estudar a virulência dessas estirpes, informação importente para o aperfeiçoamento dos métodos de controle. As estirpes emergiram do estudo caso-controle, onde as tipagens moleculares por RFLP mostraram a existência de quatro famílias de M. avium (PIG-A, B, C e D). Um estirpe representante de cada família foi inoculada pela via intra-peritoneal em 48 hamsters com uma dose de 30.000 U.F.C. por animal. Após 2, 13, 26 e 40 dias da inoculação, 12 hamsters inoculados de cada família foram anestesiados, sacrificados e os agentes foram quantificados no fígado, baço e pulmão. A presença das estirpes foi verificada no sangue e também foram realizados exames histológicos. As estipers PIG-A, B, C e D desenvolveram lesões granulomatosas no fígado e baço nos quatro tempos experimentais; disseminaram-se pela via linfo-hemática, multiplicando-se em fígado, baço e pulmão. Nos quatro tempos experimentais houve diferença entre as contagens de U.F.C./g entre os órgãos (T1: p<0,001; T2: p<0,001; T3: p<0,001 e T4: p<0,001) e as obtidas do baço foram sempre superiores às do fígado e pulmão. Nos quatro tempos experimentais houve diferença entre as contagens de U.F.C./g entre as estirpes (T1: p<0,001; T2: <0,001; T3: p<0,001 e T4: p<0,001) e foi possível construir a seguinte escala decrescente de virulência: PIG-B > PIG-A > PIG-D > PIG-C
Given that the existence of molecularly different families of M. avium circulating in swine of the south area of Brazil has been proved, and that some doubts remain regarding the importance of the horizontal transmission as mechanism of maintenance of the disease, this work aimed to study the virulence of those strains, an important information for the improvement of the control methods. The strains emerged from a case-control study, when the RFLP molecular typification showed the existence of four families of M. avium (PIG-A, B, C and D). A strain representative of each family was inoculated by intra-peritoneal route in 48 hamsters with a dose of 30.000 C.F.U./animal. After 2, 13, 26 and 40 days post-infection, 12 inoculated hamsters of each family were anesthetized, euthanized and the bacteria were quantified in the liver, spleen and lung. The presence of the strains was verified in the blood and also histological exams were accomplished. The strains PIG-A, B, C and D developed granulomatous lesions in the liver and spleen in the four experimental times; they were disseminated by the linfo-haematic route, multiplying in liver, spleen and lung. In the four experimental times there was difference among the countings of C.F.U./g among the organs (T1: p<0,001; T2: p<0,001; T3: p<0,001 and T4: p<0,001) and that obtained of the spleen were always superiors to the one of the liver and lung. In the four experimental times there was difference among the countings of C.F.U./g among the ancestries (T1: p<0,001; T2: p<0,001; T3: p<0,001 and T4: p<0,001) and was possible to build the following order of decreasing virulence: PIG-B > PIG-A > PIG-D > PIG-C