Resumo
Radiotherapy causes destruction of tumor cells, but also threatens the integrity and survival of surrounding normal cells. Then, woman submitted to irradiation for cancer treatment may present permanent ovary damage, resulting in impaired fertility. The objective of this study was to investigate the effects of therapeutic doses of ionizing radiation (IR), used for ovarian cancer treatment in humans, on bovine cumulus-oocyte complexes (COCs) as experimental model. Bovine ovaries were exposed to 0.9 Gy, 1.8 Gy, 3.6 Gy or 18.6 Gy IR, and then COCs were collected and used to evaluate: (a) oocyte nuclear maturation; (b) presence of phosphorylated H2A.X (γH2AX), as an indicator of DNA double-strand breaks (DSBs); and (c) expression of genes involved in DNA repair (TP53BP1, RAD52, ATM, XRCC6 and XRCC5) and apoptosis (BAX). The radiation doses tested in this study had no detrimental effects on nuclear maturation and did not increase γH2AX in the oocytes. However, IR treatment altered the mRNA abundance of RAD52 (RAD52 homolog, DNA repair protein) and BAX (BCL2-associated X protein). We conclude that although IR doses had no apparent effect on oocyte nuclear maturation and DNA damage, molecular pathways involved in DNA repair and apoptosis were affected by IR exposure in cumulus cells.(AU)
Assuntos
Animais , Feminino , Bovinos , Oócitos/citologia , Radiação Ionizante , Dano ao DNA , Perfilação da Expressão Gênica/veterináriaResumo
Low light intensity reduces the production of photoassimilates by plants and consequentlythe accumulation of reserves in seeds. The objective was to evaluate the physiological quality of soybean seeds produced under shading initiated at different crop phenological stages. In a completely randomized design, the experiment was carried out in a greenhouse, at CAV/UDESC, during the 2018/2019 harvest, with nine treatments and 10 replications. The treatments consisted of nine seed lots, eight of which were obtained from plants submitted to continuous 70% shading, initiated at different phenological stages: stage V6 (Day Julian 07); R1 (JD14); R2 (JD21); R3 (JD28); R4 (JD35); R5 (JD42); start of R6 (JD49); end of R6 (JD56), and a lot of seeds produced without shading (control). Each repetition consisted of a pot containing a soybean plant. The germination potential and vigor of the seeds produced were evaluated. Soybean seed germination was higher in the lot of seeds from shaded plants from the R4 stage (JD35), with 98%, but the other lots presented percentages above 87%. The vigor in the accelerated aging test was lower for the lot obtained from shaded plants from V6, with 43%. The dry mass of seedlings showed a 7.0 to 13.3% reductionin relation to the controlwhen the shading was imposed between R3 and R6. It was concluded that the continuous 70% shading in soybeans promoted higher seed germination when imposed from the R4 stage;however, the vigor was dependenton the stage that the shading started and the test used.(AU)
A baixa intensidade luminosa reduz a produção de fotoassimilados pelas plantas e consequentemente o acúmulo de reservas nas sementes. Objetivou-se avaliar a qualidade fisiológica de sementes desoja produzidassob sombreamento iniciado emdiferentes estádios fenológicos da cultura. O experimento foi conduzido em casa de vegetação, no CAV/UDESC, durante a safra 2018/2019, em delineamento inteiramente casualizado, com nove tratamentos e 10 repetições. Os tratamentos foram constituídos por nove lotes de sementes, sendo oito obtidos de plantas submetidas a sombreamento contínuode 70%, iniciado em diferentes estádios fenológicos: estádio V6 (Dia Juliano 07); R1 (DJ 14); R2 (DJ 21); R3 (DJ 28); R4 (DJ35); R5 (DJ 42); início de R6 (DJ 49); finalde R6 (DJ 56), e um lote de sementes produzidas sem sombreamento (testemunha). Cada repetição foi composta por um vaso contendo uma planta de soja. Avaliou-se o potencial de germinação e vigor das sementes produzidas. Agerminação de sementes de soja foi maior no lote de sementes oriundas de plantas sombreadas a partir do estádio R4 (35 DJ), com 98%, mas, os demais lotes apresentaram percentuais acima de 87%. O vigor no teste de envelhecimento acelerado foi inferior parao loteobtido de plantas sombreadasa partir de V6, com 43%. Já a massa seca de plântulas apresentou redução de 7,0 a 13,3% em relação à testemunha, quando o sombreamento foi imposto entre R3 e R6. Concluiu-se que o sombreamento contínuo de 70% em soja promoveu maior germinação de sementes quando imposto a partir do estádio R4, porém, o vigor foi afetado de maneira dependente do estádio que o sombreamento iniciou e do teste utilizado.(AU)
Assuntos
Fotossíntese/fisiologia , Sementes/fisiologia , Glycine max/fisiologia , Técnica Histológica de Sombreamento/métodos , Radiação SolarResumo
The objective of this work was to compare the dry matter intake, milk yield and quality, physiological and biochemical parameters in Holstein (n=10) and Jersey (n=10) cows under heat stress and insolation, in two treatments: CL - cooling by ventilation and sprinkling and HS - heat stress and insolation. Data were submitted to ANOVA. There was an interaction between treatment and breed and day effect for dry matter intake. For consumption in % of body weight, CL and Jersey cows consumed more. CL cows produced more milk and 3.5% fat-corrected milk. Feed efficiency was similar between treatments and breeds. Fat, lactose, total solids, and somatic cell score did not differ. The concentration of milk urea nitrogen was higher for CL cows. Milk from Holstein cows had greater stability to alcohol, and from HT cows had a greater freezing point of milk. HT cows had higher respiratory rates in the morning and surface temperatures in the afternoon. There were no differences in beta-hydroxybutyrate and glucose concentrations. Heat stress, with insulation, reduces intake, especially in Holstein cows, as well as milk production and increases the freezing point of milk, respiratory rate, and surface temperature.
O objetivo deste trabalho foi comparar o consumo de matéria seca, a produção e a qualidade do leite, os parâmetros fisiológicos e bioquímicos em vacas das raças Holandesa (n=10) e Jersey (n=10) sob estresse calórico e insolação, em dois tratamentos: CL - resfriamento por ventilação e aspersão; HS - estresse térmico e insolação. Os dados foram submetidos à análise de variância. Houve interação entre tratamento e raça e efeito de dia para consumo de matéria seca. Para consumo em % de peso vivo, vacas CL e Jersey consumiram mais. Vacas CL produziram mais leite e leite corrigido a 3,5% de gordura. A eficiência alimentar foi similar entre tratamentos e raças. Teores de gordura, lactose, sólidos totais e escore de células somáticas não diferiram. A concentração de nitrogênio ureico do leite foi maior para vacas CL. O leite das vacas Holandesas apresentou maior estabilidade ao álcool, e de vacas HT maior crioscopia. Vacas HT apresentaram maior frequência respiratória de manhã e temperatura superficial à tarde. Não houve diferenças para concentração de beta-hidroxibutirato e glicose. O estresse calórico, com insolação, reduz o consumo, especialmente em vacas Holandesas, bem como a produção de leite, com aumento da crioscopia, elevando a frequência respiratória e a temperatura superficial.
Assuntos
Animais , Bovinos , Insolação , Radiação Solar , Leite/química , Temperatura Alta/efeitos adversosResumo
Watermelon is a crop highly dependent on bees for pollination, and environmental conditions are some of the most important factors affecting bee foraging. In this study, we analyze the effect of environmental conditions on the behavior of the most common bees visiting flowers of watermelon crops in Panama. We recorded the number of visits, visit duration, and the corresponding environmental conditions during the visits. Environmental conditions affected the observed groups of bees differently: honey bee visit proportion was remarkably higher at low temperatures, solar radiation, wind speed, and high relative humidity, early in the morning when they made about 90% of their flower visits. The other observed bees showed a more homogenous behavior during the day, with peaks representing about 25-35% of the daily visits. Visit number showed a correlation with temperature for all the most common bees except Augochloropsis spp., with solar radiation for A. mellifera and Lasioglossum spp., with humidity for all except Lasioglossum spp., and with wind speed for all of the analyzed bees. Visit durations were remarkably longer in N. perilampoides early on the day. At the same time, the rest of the bees showed less pronounced duration peaks, reaching their maximum at intermediate values of environmental conditions. Visit duration on honeybees did not correlate with environmental factors, but it did for most other bees. Environmental conditions showed a strong effect on the bee foraging behavior, with each group of bees concentrating their activities in favorable conditions depending on their biology, establishing their daily foraging patterns.
Assuntos
Abelhas , Comportamento Animal , Efeitos do Clima , Citrullus , Polinização , PanamáResumo
Squamous cell carcinoma (SCC) is a common neoplastic skin disease that is highly prevalent in tropical countries. As the skin has a variety of cells, overexposure to environmental factors, such as ultraviolet light, can affect this organ, resulting in malignancies, such as cutaneous SCC, hemangioma, and hemangiosarcoma. SCC arises from keratinocytes in the skin and is locally invasive with low metastatic rates, commonly affecting unpigmented skin in sites with high exposure to sunlight, such as ventral regions. SCC has a variable etiology that is not well understood. Therefore, literature review aimed to critically evaluate the risk factors involved in the SCC development.(AU)
Assuntos
Animais , Cães , Raios Ultravioleta , Carcinoma de Células Escamosas , Fatores de Risco , Hemangioma , Hemangiossarcoma , NeoplasiasResumo
Pogostemon cablin (Blanco) Benth. is an aromatic species popularly known as patchouli. The essential oil rich in patchoulol extracted from leaves is used by the pharmaceutical industries. The objective was to investigate the effects of shade nets and shading intensities on P. cablin growth, anatomy, chemical composition and essential oil content. The experiment was conducted with two types of shade nets (black and Aluminet), three light intensities (30, 50, and 70% shading) and full sun. The different light intensities influenced the growth and the essential oil yield, and chemical composition. Patchouli plants grown in an environment with shading showed higher values for the leaf, stem, root ant total dry weights compared to full sun. At 50% of shading, Aluminet provided gain in stem and total dry weights. Aluminet and black net at 50 and 70% of shading enhance essential oil yield. Patchoulol and pogostol contents were higher under full sun, and, black net and Aluminet at 50% shading. The environment for patchouli cultivation can be improved by use shade nets, especially with Aluminet at 50% of shading.
Pogostemon cablin (Blanco) Benth. é uma espécie aromática popularmente conhecida como patchouli. O óleo essencial rico em patchoulol extraído de folhas é utilizado pelas indústrias farmacêuticas. O objetivo foi investigar os efeitos das malhas e intensidades de sombreamento no crescimento, anatomia, composição química e teor de óleo essencial de P. cablin. O experimento foi conduzido com dois tipos de malhas (preta e Aluminet), três intensidades de luz (30, 50 e 70% de sombreamento) e pleno sol. As diferentes intensidades de luz influenciaram o crescimento, o rendimento e a composição química do óleo essencial. Plantas de patchouli cultivadas em ambiente sombreado apresentaram maiores valores para os pesos secos de folha, caule, raiz e total em relação ao pleno sol. Com 50% de sombreamento, Aluminet proporcionou ganho de peso seco de caule e total. Aluminet e malha preta a 50 e 70% de sombreamento aumentam o rendimento do óleo essencial. Os teores de patchoulol e pogostol foram maiores a pleno sol, malha preta e Aluminet com 50% de sombreamento. O ambiente para o cultivo do patchouli pode ser melhorado com o uso de malhas de sombreamento, especialmente com Aluminet em 50% de sombreamento.
Assuntos
Iluminação/efeitos adversos , Óleos Voláteis/química , Pogostemon/anatomia & histologia , Pogostemon/crescimento & desenvolvimentoResumo
Tetanus toxin blocks the release of the inhibitory neurotransmitters in the central nervous system and causes tetanus and its main form of prevention is through vaccination. The vaccine is produced by inactivation of tetanus toxin with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potential immunogenic response and to reduce the post-vaccination side effects. Therefore, the aim of this study was to characterize the tetanus toxin structure after different doses of ionizing radiation of 60Co. Methods Irradiated and native tetanus toxin was characterized by SDS PAGE in reducing and non-reducing conditions and MALD-TOF. Enzymatic activity was measured by FRET substrate. Also, antigenic properties were assessed by ELISA and Western Blot data. Results Characterization analysis revealed gradual modification on the tetanus toxin structure according to doses increase. Also, fragmentation and possible aggregations of the protein fragments were observed in higher doses. In the analysis of peptide preservation by enzymatic digestion and mass spectrometry, there was a slight modification in the identification up to the dose of 4 kGy. At subsequent doses, peptide identification was minimal. The analysis of the enzymatic activity by fluorescence showed 35 % attenuation in the activity even at higher doses. In the antigenic evaluation, anti-tetanus toxin antibodies were detected against the irradiated toxins at the different doses, with a gradual decrease as the dose increased, but remaining at satisfactory levels. Conclusion Ionizing radiation promoted structural changes in the tetanus toxin such as fragmentation and/or aggregation and attenuation of enzymatic activity as the dose increased, but antigenic recognition of the toxin remained at good levels indicating its possible use as an immunogen. However, studies of enzymatic activity of tetanus toxin irradiated with doses above 8 kGy should be further analyzed.(AU)
Assuntos
Radiação Ionizante , Tétano , Ensaio de Imunoadsorção Enzimática , Raios gama , Toxina Tetânica , CobaltoResumo
Tetanus toxin blocks the release of the inhibitory neurotransmitters in the central nervous system and causes tetanus and its main form of prevention is through vaccination. The vaccine is produced by inactivation of tetanus toxin with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potential immunogenic response and to reduce the post-vaccination side effects. Therefore, the aim of this study was to characterize the tetanus toxin structure after different doses of ionizing radiation of 60Co. Methods Irradiated and native tetanus toxin was characterized by SDS PAGE in reducing and non-reducing conditions and MALD-TOF. Enzymatic activity was measured by FRET substrate. Also, antigenic properties were assessed by ELISA and Western Blot data. Results Characterization analysis revealed gradual modification on the tetanus toxin structure according to doses increase. Also, fragmentation and possible aggregations of the protein fragments were observed in higher doses. In the analysis of peptide preservation by enzymatic digestion and mass spectrometry, there was a slight modification in the identification up to the dose of 4 kGy. At subsequent doses, peptide identification was minimal. The analysis of the enzymatic activity by fluorescence showed 35 % attenuation in the activity even at higher doses. In the antigenic evaluation, anti-tetanus toxin antibodies were detected against the irradiated toxins at the different doses, with a gradual decrease as the dose increased, but remaining at satisfactory levels. Conclusion Ionizing radiation promoted structural changes in the tetanus toxin such as fragmentation and/or aggregation and attenuation of enzymatic activity as the dose increased, but antigenic recognition of the toxin remained at good levels indicating its possible use as an immunogen. However, studies of enzymatic activity of tetanus toxin irradiated with doses above 8 kGy should be further analyzed.(AU)
Assuntos
Radiação Ionizante , Tétano , Ensaio de Imunoadsorção Enzimática , Raios gama , Toxina Tetânica , CobaltoResumo
A betaterapia é uma forma de braquiterapia onde a fonte emissora de raios beta é posicionada diretamente na área em que se deseja tratar. As principais aplicações são em dermatologia e em oftalmologia no tratamento de lesões superficiais, pois as partículas betas têm grande poder de ionização e pequeno alcance no tecido. A radioterapia em medicina veterinária vem aumentando e apresentando resultados satisfatórios no Brasil e pelo mundo. Esta revisão teve como objetivo descrever os princípios e aplicações da betaterapia em medicina veterinária. Para a concretização foi realizada uma pesquisa bibliográfica, possibilitando assim, consolidar informações relativas à base teórica e ao tema proposto. Foi observado o uso do Estrôncio-90 (Sr90) nos principais procedimentos realizados em animais domésticos, selvagens e de experimentação, realçando a importância desta técnica adjuvante. A betaterapia mostrou-se exequível e de fácil manuseio para aplicações em medicina veterinária, especialmente nos casos de lesões e neoplasias superficiais. As fontes radioativas emissoras de partículas betas, como as de Sr90 não requerem grandes cuidados com proteção radiológica, o que contribui para a viabilidade da sua manipulação em radioterapia veterinária, principalmente nos procedimentos intra-operatórios.
Beta therapy is a form of brachytherapy where the beta ray emitting source is positioned directly in the area in which it is desired to be treated. The main applications are in dermatology and ophthalmology in the treatment of superficial lesions, since beta particles have great ionization power and small reach in the tissue. Radiotherapy in veterinary medicine has been increasing and presenting results satisfactory in Brazil and around the world. This review aimed to describe the principles and applications of beta-therapy in veterinary medicine. For the realization, a bibliographic research was carried through, making possible, to consolidate information related to the theoretical base and to the proposed theme. The use of Strontium-90 (Sr90) was observed in the main procedures performed on domestic, wild and experimental animals, highlighting the importance of this adjuvant technique. Beta therapy has been shown to be feasible and easy to handle for veterinary medicine applications, especially in cases of superficial lesions and neoplasms. Beta-emitting radioactive sources, such as those of Sr90, do not require great care with radiological protection, which contributes to the viability of their manipulation in veterinary radiotherapy, especially in intraoperative procedures.
La terapia beta es una forma de braquiterapia en la que la fuente emisora de rayos beta se coloca directamente en el área en la que se desea tratar. Las principales aplicaciones son en dermatología y oftalmología en el tratamiento de lesiones superficiales, porque las partículas beta tienen un gran poder de ionización y un alcance pequeño en el tejido. La radioterapia en medicina veterinaria ha ido aumentando y presentando resultados considerablemente en Brasil y en todo el mundo. Esta revisión tuvo como objetivo describir los principios y aplicaciones de la terapia beta en medicina veterinaria. Para la realización, se realizó una investigación bibliográfica que permitió consolidar información relacionada con la base teórica y el tema propuesto. El uso de Estroncio-90 (Sr90) se observó en los principales procedimientos realizados en animales domésticos, salvajes y experimentales, destacando la importancia de esta técnica adyuvante. Se ha demostrado que la terapia beta es factible y fácil de manejar para aplicaciones veterinarias, especialmente en casos de lesiones superficiales y neoplasias. Las fuentes radiactivas emisoras beta, como las de Sr90, no requieren un gran cuidado con la protección radiológica, lo que contribuye a la viabilidad de su manipulación en radioterapia veterinaria, especialmente en procedimientos intraoperatorios.
Assuntos
Animais , Braquiterapia/história , Braquiterapia/veterinária , Partículas beta/uso terapêutico , Radioisótopos de Estrôncio/uso terapêutico , Radioterapia/veterináriaResumo
Six different bread wheat genotypes; two Egyptian commercial varieties (control); Giza-168 and Gemmeiza-11, and four promising lines; L84 and L148, resulted via hybridization and M10 and M34 via radiation mutation program) were rheologically evaluated using extensograph and for protein, analysis using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The radiation mutant M10 and M34 had the highest maximum resistance which is a very good indicator of strong gluten. The amount of gluten content was higher in M10, L148, and M34 compared to the control samples Gz168 and Gm11. Sulfide amino acids (CYS and MET) are slightly higher in M10. The electrophoretic results and amino acid analyzers show that the best technological quality was exhibited by M10. Radiation mutants wheat genotypes have a protein with good characteristics, mainly gluten which is significantly higher compared to control samples. The rheological properties measured as extensograph and gel electrophoresis were much better in irradiated lines M10 and M34.(AU)
Seis diferentes genótipos de trigo de pão, duas variedades comerciais egípcias (controle) Giza-168 e Gemmeiza-11 e quatro linhas promissoras L84 e L148, obtidas via hibridação, e M10 e M34, via programa de mutação por radiação foram avaliados reologicamente por meio de extensógrafo, enquanto, para proteínas, foram feitas análises utilizando eletroforese em gel de poliacrilamida com dodecilsulfato de sódio (SDS-PAGE). Os mutantes de radiação M10 e M34 apresentaram a maior resistência máxima, o que é um indicador muito bom de glúten forte. A quantidade de glúten foi maior em M10, L148 e M34 em comparação com as amostras de controle Gz168 e Gm11. Os aminoácidos sulfurados (CYS e MET) são um pouco mais altos no M10. Os resultados eletroforéticos e analisadores de aminoácidos mostram que a melhor qualidade tecnológica foi exibida pelo M10. Os genótipos de trigo mutantes da radiação possuem uma proteína com boas características, principalmente o glúten, que é significativamente maior em comparação às amostras do grupo controle. As propriedades reológicas medidas, como extensógrafo e eletroforese em gel, foram muito melhores nas linhas irradiadas M10 e M34.(AU)
Assuntos
Triticum/genética , Triticum/efeitos da radiação , Genótipo , Mutação , GlutensResumo
Plants adjust their shoot growth to acclimate to changing environmental factors, such as to enhanced Ultraviolet-B (UV-B) radiation. However, people have ignored that plant roots can also respond to UV-B light. Here, we find the morphology curled wheat roots under UV-B radiation, that we call, bending roots. The curly region is the transition zone of the root after observed at the cellular level. After exposed to enhanced UV-B radiation for 2 d (10.08 KJ/m2/d), cell size decreased and actin filaments gathered in wheat roots. We also find that H2O2 production increased and that content of the indole-3-acetic acid (IAA) increased remarkably. The pharmacological experiment revealed that actin filaments gathered and polymerized into bundles in the wheat root cells after irrigated H2O2 and IAA. These results indicated that actin filaments changed their distribution and formed the bending root, which was related to H2O2 production and increase in IAA. Overall, actin filaments in wheat root cells could be a subcellular target of UV-B radiation, and its disruption determines root morphology.(AU)
As plantas ajustam o crescimento da parte aérea para se adaptarem a fatores ambientais variáveis, como o aumento da radiação ultravioleta B (UVB). No entanto, as pessoas ignoram que as raízes das plantas também podem responder à luz UVB. Neste estudo, verificamos a morfologia das raízes enroladas de trigo sob radiação UVB, o que chamamos de raízes dobradas. A região encaracolada é a zona de transição da raiz no nível celular. Depois de exposição à radiação UVB aprimorada por 2 dias (10,08 KJ/m2/d), o tamanho das células diminuiu, e os filamentos de actina se reuniram. Também constatamos que a produção de H2O2 aumentou e que o conteúdo do ácido indol-3-acético (IAA) aumentou notavelmente. O experimento farmacológico revelou que os filamentos de actina se reuniram e polimerizaram em feixes nas células da raiz de trigo após irrigação com H2O2 e IAA. Esses resultados indicam que os filamentos de actina alteraram sua distribuição e formaram a raiz dobrada, relacionada à produção de H2O2 e ao aumento do IAA. No geral, os filamentos de actina nas células da raiz de trigo podem ser um alvo subcelular da radiação UVB, e sua interrupção determina a morfologia da raiz.(AU)
Assuntos
Triticum/efeitos da radiação , Raízes de Plantas/efeitos da radiação , Actinas , Raios Ultravioleta/efeitos adversosResumo
A betaterapia é uma forma de braquiterapia onde a fonte emissora de raios beta é posicionada diretamente na área em que se deseja tratar. As principais aplicações são em dermatologia e em oftalmologia no tratamento de lesões superficiais, pois as partículas betas têm grande poder de ionização e pequeno alcance no tecido. A radioterapia em medicina veterinária vem aumentando e apresentando resultados satisfatórios no Brasil e pelo mundo. Esta revisão teve como objetivo descrever os princípios e aplicações da betaterapia em medicina veterinária. Para a concretização foi realizada uma pesquisa bibliográfica, possibilitando assim, consolidar informações relativas à base teórica e ao tema proposto. Foi observado o uso do Estrôncio-90 (Sr90) nos principais procedimentos realizados em animais domésticos, selvagens e de experimentação, realçando a importância desta técnica adjuvante. A betaterapia mostrou-se exequível e de fácil manuseio para aplicações em medicina veterinária, especialmente nos casos de lesões e neoplasias superficiais. As fontes radioativas emissoras de partículas betas, como as de Sr90 não requerem grandes cuidados com proteção radiológica, o que contribui para a viabilidade da sua manipulação em radioterapia veterinária, principalmente nos procedimentos intra-operatórios.(AU)
Beta therapy is a form of brachytherapy where the beta ray emitting source is positioned directly in the area in which it is desired to be treated. The main applications are in dermatology and ophthalmology in the treatment of superficial lesions, since beta particles have great ionization power and small reach in the tissue. Radiotherapy in veterinary medicine has been increasing and presenting results satisfactory in Brazil and around the world. This review aimed to describe the principles and applications of beta-therapy in veterinary medicine. For the realization, a bibliographic research was carried through, making possible, to consolidate information related to the theoretical base and to the proposed theme. The use of Strontium-90 (Sr90) was observed in the main procedures performed on domestic, wild and experimental animals, highlighting the importance of this adjuvant technique. Beta therapy has been shown to be feasible and easy to handle for veterinary medicine applications, especially in cases of superficial lesions and neoplasms. Beta-emitting radioactive sources, such as those of Sr90, do not require great care with radiological protection, which contributes to the viability of their manipulation in veterinary radiotherapy, especially in intraoperative procedures.(AU)
La terapia beta es una forma de braquiterapia en la que la fuente emisora de rayos beta se coloca directamente en el área en la que se desea tratar. Las principales aplicaciones son en dermatología y oftalmología en el tratamiento de lesiones superficiales, porque las partículas beta tienen un gran poder de ionización y un alcance pequeño en el tejido. La radioterapia en medicina veterinaria ha ido aumentando y presentando resultados considerablemente en Brasil y en todo el mundo. Esta revisión tuvo como objetivo describir los principios y aplicaciones de la terapia beta en medicina veterinaria. Para la realización, se realizó una investigación bibliográfica que permitió consolidar información relacionada con la base teórica y el tema propuesto. El uso de Estroncio-90 (Sr90) se observó en los principales procedimientos realizados en animales domésticos, salvajes y experimentales, destacando la importancia de esta técnica adyuvante. Se ha demostrado que la terapia beta es factible y fácil de manejar para aplicaciones veterinarias, especialmente en casos de lesiones superficiales y neoplasias. Las fuentes radiactivas emisoras beta, como las de Sr90, no requieren un gran cuidado con la protección radiológica, lo que contribuye a la viabilidad de su manipulación en radioterapia veterinaria, especialmente en procedimientos intraoperatorios.(AU)
Assuntos
Animais , Partículas beta/uso terapêutico , Radioterapia/veterinária , Braquiterapia/história , Braquiterapia/veterinária , Radioisótopos de Estrôncio/uso terapêuticoResumo
This study aimed to evaluate the production characteristics of pastures in integrated livestock production systems. For that, an experiment was carried out in São Carlos, SP, Brazil, from 2013 to 2015. Forage development, production and nutritive value were evaluated in five beef cattle production systems: extensive continuous stocking (Urochloa decumbens) = EXT; intensive = INT; crop-livestock = iCL; livestock-forest = iLF and crop-livestock-forest = iCLF. Rotational stocking pastures in INT, iCL, iLF and iCLF systems were established with Urochloa brizantha cv. BRS Piatã. In iCL and iCLF, pastures were renovated by resowing the grass simultaneously with corn. In iLF and iCLF, eucalyptus (Eucalyptus urograndis clone GG100) was planted in Apr 2011 in single rows with 15 × 2 m spacing. In the 2013/2014 crop season, INT, iCL, and iCLF pastures were more productive than in iLF and EXT. Shading increase in the 2014/2015 season reduced pasture production in iLF and iCLF, compared with INT and iCL, but increased crude protein content and digestibility. In the shaded systems, pasture production was affected by proximity to trees, mainly due to reductions in solar radiation transmission. The principal component analyses showed that forage accumulation and leaf area index were associated with the position in the middle of the inter-row, and nutritive value was associated with the position at 1.5 m from the trees. In iCLF, solar radiation transmission greater than 60 % maintained forage accumulation similar to iCL, while in iLF, it reduced forage accumulation, evidencing that pasture renovation minimized shading effects in these systems.
Assuntos
Masculino , Bovinos , Agricultura Florestal/métodos , Criação de Animais Domésticos/métodos , Eucalyptus , Luz Solar , Pastagens/métodos , Árvores , Integração de SistemasResumo
This study aimed to evaluate the production characteristics of pastures in integrated livestock production systems. For that, an experiment was carried out in São Carlos, SP, Brazil, from 2013 to 2015. Forage development, production and nutritive value were evaluated in five beef cattle production systems: extensive continuous stocking (Urochloa decumbens) = EXT; intensive = INT; crop-livestock = iCL; livestock-forest = iLF and crop-livestock-forest = iCLF. Rotational stocking pastures in INT, iCL, iLF and iCLF systems were established with Urochloa brizantha cv. BRS Piatã. In iCL and iCLF, pastures were renovated by resowing the grass simultaneously with corn. In iLF and iCLF, eucalyptus (Eucalyptus urograndis clone GG100) was planted in Apr 2011 in single rows with 15 × 2 m spacing. In the 2013/2014 crop season, INT, iCL, and iCLF pastures were more productive than in iLF and EXT. Shading increase in the 2014/2015 season reduced pasture production in iLF and iCLF, compared with INT and iCL, but increased crude protein content and digestibility. In the shaded systems, pasture production was affected by proximity to trees, mainly due to reductions in solar radiation transmission. The principal component analyses showed that forage accumulation and leaf area index were associated with the position in the middle of the inter-row, and nutritive value was associated with the position at 1.5 m from the trees. In iCLF, solar radiation transmission greater than 60 % maintained forage accumulation similar to iCL, while in iLF, it reduced forage accumulation, evidencing that pasture renovation minimized shading effects in these systems.(AU)
Assuntos
Masculino , Bovinos , Criação de Animais Domésticos/métodos , Árvores , Pastagens/métodos , Luz Solar , Eucalyptus , Agricultura Florestal/métodos , Integração de SistemasResumo
This case report describes the outcome of treatment of dogs with advanced (deeply invasive) nasal planum squamous cell carcinomas with palliative or definitive radiation therapy. All dogs were diagnosed with nasal planum squamous cell carcinoma by histopathology, and their owners declined aggressive surgery. Dogs were treated with a cobalt-60 unit, definitive-intent radiation protocol consisting of 15 daily fractions (Monday-Friday) of 2.8 Gy, and palliative radiation protocol consisting of 4 fractions of 8 Gy performed once a week. Three dogs had T3 tumors and three had T4 tumors according to the WHO staging system. Two dogs had tumor complete remission and four had tumor partial remission. Survival time of dogs submitted to radiation therapy was 95-417 days. Radiation acute side effects involved only mild epilation and cutaneous erythema in palliative protocols, and moist desquamation, epilation, mild rhinitis and oral mucositis in definitive-intent RT. Radiation therapy, especially palliative protocols, can be a treatment option for nasal planum squamous cell carcinoma in dogs, when the owner declines aggressive surgery. It may contribute to partial or complete tumor remission and better patient quality of life, even at advanced stages, leading to mild side effects.(AU)
Este relato descreve seis casos de carcinoma de células escamosas de plano nasal canino tratados com radioterapia definitiva ou paliativa. Em todos os casos, o diagnóstico definitivo foi feito mediante biópsia, e os tutores declinaram da cirurgia agressiva. A radioterapia foi realizada utilizando-se um equipamento de cobalto-60, e o protocolo definitivo consistiu de 15 frações de 2,8 Gy, cinco vezes por semana (segunda a sexta), enquanto o protocolo paliativo consistiu de quatro frações de 8 Gy, uma vez por semana. Seis cães foram tratados, entre os quais três tinham tumores em estágio T3 e três em estágio T4, de acordo com a tabela TNM da Organização Mundial da Saúde. Dois animais apresentaram remissão completa do tumor após o tratamento e quatro apresentaram remissão parcial. O tempo de sobrevida variou entre 95-417 dias, sendo que dois animais ainda estão vivos e em observação. Os efeitos colaterais da radioterapia foram apenas epilação e eritema leve, no protocolo paliativo, e epilação, radiodermite úmida, rinite e mucosite oral, no protocolo definitivo. A radioterapia, especialmente no protocolo paliativo, pode ser considerada uma opção de tratamento quando os tutores declinam da cirurgia agressiva. Ela pode contribuir para remissão (parcial ou completa) do tumor e melhor qualidade de vida do paciente, mesmo nos casos avançados, levando a efeitos colaterais mínimos.(AU)
Assuntos
Animais , Cães , Neoplasias Nasais/veterinária , Carcinoma de Células Escamosas de Cabeça e Pescoço/radioterapia , Carcinoma de Células Escamosas de Cabeça e Pescoço/veterinária , Cuidados PaliativosResumo
This case report describes the outcome of treatment of dogs with advanced (deeply invasive) nasal planum squamous cell carcinomas with palliative or definitive radiation therapy. All dogs were diagnosed with nasal planum squamous cell carcinoma by histopathology, and their owners declined aggressive surgery. Dogs were treated with a cobalt-60 unit, definitive-intent radiation protocol consisting of 15 daily fractions (Monday-Friday) of 2.8 Gy, and palliative radiation protocol consisting of 4 fractions of 8 Gy performed once a week. Three dogs had T3 tumors and three had T4 tumors according to the WHO staging system. Two dogs had tumor complete remission and four had tumor partial remission. Survival time of dogs submitted to radiation therapy was 95-417 days. Radiation acute side effects involved only mild epilation and cutaneous erythema in palliative protocols, and moist desquamation, epilation, mild rhinitis and oral mucositis in definitive-intent RT. Radiation therapy, especially palliative protocols, can be a treatment option for nasal planum squamous cell carcinoma in dogs, when the owner declines aggressive surgery. It may contribute to partial or complete tumor remission and better patient quality of life, even at advanced stages, leading to mild side effects.(AU)
Este relato descreve seis casos de carcinoma de células escamosas de plano nasal canino tratados com radioterapia definitiva ou paliativa. Em todos os casos, o diagnóstico definitivo foi feito mediante biópsia, e os tutores declinaram da cirurgia agressiva. A radioterapia foi realizada utilizando-se um equipamento de cobalto-60, e o protocolo definitivo consistiu de 15 frações de 2,8 Gy, cinco vezes por semana (segunda a sexta), enquanto o protocolo paliativo consistiu de quatro frações de 8 Gy, uma vez por semana. Seis cães foram tratados, entre os quais três tinham tumores em estágio T3 e três em estágio T4, de acordo com a tabela TNM da Organização Mundial da Saúde. Dois animais apresentaram remissão completa do tumor após o tratamento e quatro apresentaram remissão parcial. O tempo de sobrevida variou entre 95-417 dias, sendo que dois animais ainda estão vivos e em observação. Os efeitos colaterais da radioterapia foram apenas epilação e eritema leve, no protocolo paliativo, e epilação, radiodermite úmida, rinite e mucosite oral, no protocolo definitivo. A radioterapia, especialmente no protocolo paliativo, pode ser considerada uma opção de tratamento quando os tutores declinam da cirurgia agressiva. Ela pode contribuir para remissão (parcial ou completa) do tumor e melhor qualidade de vida do paciente, mesmo nos casos avançados, levando a efeitos colaterais mínimos.(AU)
Assuntos
Animais , Cães , Neoplasias Nasais/veterinária , Carcinoma de Células Escamosas de Cabeça e Pescoço/radioterapia , Carcinoma de Células Escamosas de Cabeça e Pescoço/veterinária , Cuidados PaliativosResumo
Abstract Plants adjust their shoot growth to acclimate to changing environmental factors, such as to enhanced Ultraviolet-B (UV-B) radiation. However, people have ignored that plant roots can also respond to UV-B light. Here, we find the morphology curled wheat roots under UV-B radiation, that we call, bending roots. The curly region is the transition zone of the root after observed at the cellular level. After exposed to enhanced UV-B radiation for 2 d (10.08 KJ/m2/d), cell size decreased and actin filaments gathered in wheat roots. We also find that H2O2 production increased and that content of the indole-3-acetic acid (IAA) increased remarkably. The pharmacological experiment revealed that actin filaments gathered and polymerized into bundles in the wheat root cells after irrigated H2O2 and IAA. These results indicated that actin filaments changed their distribution and formed the bending root, which was related to H2O2 production and increase in IAA. Overall, actin filaments in wheat root cells could be a subcellular target of UV-B radiation, and its disruption determines root morphology.
Resumo As plantas ajustam o crescimento da parte aérea para se adaptarem a fatores ambientais variáveis, como o aumento da radiação ultravioleta B (UVB). No entanto, as pessoas ignoram que as raízes das plantas também podem responder à luz UVB. Neste estudo, verificamos a morfologia das raízes enroladas de trigo sob radiação UVB, o que chamamos de raízes dobradas. A região encaracolada é a zona de transição da raiz no nível celular. Depois de exposição à radiação UVB aprimorada por 2 dias (10,08 KJ/m2/d), o tamanho das células diminuiu, e os filamentos de actina se reuniram. Também constatamos que a produção de H2O2 aumentou e que o conteúdo do ácido indol-3-acético (IAA) aumentou notavelmente. O experimento farmacológico revelou que os filamentos de actina se reuniram e polimerizaram em feixes nas células da raiz de trigo após irrigação com H2O2 e IAA. Esses resultados indicam que os filamentos de actina alteraram sua distribuição e formaram a raiz dobrada, relacionada à produção de H2O2 e ao aumento do IAA. No geral, os filamentos de actina nas células da raiz de trigo podem ser um alvo subcelular da radiação UVB, e sua interrupção determina a morfologia da raiz.
Resumo
This article presents the results of the migration of radionuclides along the soil-water-feed-animal livestock chain in the adjacent territories of the former Semipalatinsk nuclear test site (SNTS). To analyze the radio ecological situation in the territory adjacent to the SNTS, 6 stationary control points were created, from which samples of soil, water, vegetation, and animal products were taken. The radiometric method was used to determine the radioactive background of the studied areas. Gamma-, alpha-spectrometry methods were used to study the content of radionuclides. The radionuclide transfer along the soil-water-feed-animal livestock chain wasdetermined by a calculation method and also veterinary and sanitary examination of the beef selected from these items. The research results show that in all the test points under studythe EDR values, the beta-particles flux density is below the limit of permissible values. The samples detected radionuclides as Am-241, Cs-137 and Pu-239/240 not exceeding the standards established by the Hygienic standardsof the Republic of Kazakhstan. The chain of radionuclides was transferred to animal products. The organoleptic assessment of the quality of cattle meat showed that inconsistencies are observed: a slight increase in lymph nodes, muddy broth, and surface moisture of muscle.(AU)
Assuntos
Animais , Bovinos , Carne Vermelha/análise , Carne Vermelha/intoxicação , Carne Vermelha/toxicidade , Elementos Radioativos/intoxicação , Elementos Radioativos/análise , RadioatividadeResumo
This article presents the results of the migration of radionuclides along the soil-water-feed-animal livestock chain in the adjacent territories of the former Semipalatinsk nuclear test site (SNTS). To analyze the radio ecological situation in the territory adjacent to the SNTS, 6 stationary control points were created, from which samples of soil, water, vegetation, and animal products were taken. The radiometric method was used to determine the radioactive background of the studied areas. Gamma-, alpha-spectrometry methods were used to study the content of radionuclides. The radionuclide transfer along the soil-water-feed-animal livestock chain wasdetermined by a calculation method and also veterinary and sanitary examination of the beef selected from these items. The research results show that in all the test points under studythe EDR values, the beta-particles flux density is below the limit of permissible values. The samples detected radionuclides as Am-241, Cs-137 and Pu-239/240 not exceeding the standards established by the Hygienic standardsof the Republic of Kazakhstan. The chain of radionuclides was transferred to animal products. The organoleptic assessment of the quality of cattle meat showed that inconsistencies are observed: a slight increase in lymph nodes, muddy broth, and surface moisture of muscle.
Assuntos
Animais , Bovinos , Carne Vermelha/análise , Carne Vermelha/intoxicação , Carne Vermelha/toxicidade , Elementos Radioativos/análise , Elementos Radioativos/intoxicação , RadioatividadeResumo
Cotton cultivation in Brazil tends to occur mostly in the rainy season, and at the time of developing the crop there are often prolonged periods of low solar radiation. The objective of this work was to evaluate the root development of cotton cultivars subjected to shading in the initial phase. The experiment was carried out in a greenhouse, using rhizotrons. The experimental design was a 2x2 factorial in randomized blocks with five replications. The treatments were composed of cotton cultivars TMG 1WS (early) and TMG 8WS (late) and luminosity conditions: non-shaded and shaded (50% reduction of luminosity). There were no interactions between the factors shading and cultivars for any parameter evaluated. Shading reduced total and lateral root growth from the 21 days after emergence (DAE) and the main root at 24DAE. Shading caused reductions in length (60%), surface (65%), mean diameter (15%), root volume (69%), root dry matter mass (83%) and shoot (64%), as well as decreasing the content (36%) and the accumulation of carbohydrates in the shoot (73%). Shading at early cotton development reduces the shoot carbohydrates production, affecting dry matter accumulation and root growth, and this response is independent of the cultivar.(AU)
O cultivo do algodoeiro no Brasil está concentrado no período chuvoso, e no momento do estabelecimento da lavoura frequentemente há períodos prolongados de baixa radiação solar. O objetivo deste trabalho foi avaliar o desenvolvimento radicular de cultivares de algodoeiro submetidos ao sombreamento na fase inicial da cultura. O experimento foi realizado em ambiente controlado, em rizotrons. O delineamento experimental foi um fatorial 2x2 em blocos ao acaso com cinco repetições. Os tratamentos foram as cultivares de algodão TMG 11WS (precoce) e TMG 81WS (tardia) e as condições de sombra: sem sombra e com sombra (50% de redução da luminosidade). Não houve interação entre os fatores sombra e cultivares estudadas para nenhum parâmetro avaliado. A sombra reduziu o crescimento radicular total e das raízes laterais a partir dos 21 dias de emergência (DAE) e da raiz principal aos 24DAE. O sombreamento reduziu o comprimento (60%), a superfície (65%), o diâmetro médio (15%), o comprimento por volume (60%), o volume radicular (69%), a massa de matéria de seca da raiz (83%) e da parte aérea (64%), o teor (36%) e o acúmulo de carboidratos na parte aérea (73%). Em conclusão, o sombreamento na fase inicial de desenvolvimento do algodoeiro reduz a produção de carboidratos da parte aérea, afetando o acúmulo de matéria seca e o crescimento radicular, sendo que essa resposta independe da cultivar.(AU)