Resumo

The aim of this study was to evaluate the morphometric parameters and the mass of adipose tissues of rats fed with cactacea flour. The animals were divided into five groups:C (Control), H (Hypercaloric diet), HP (Hypercaloric diet with 5% Pereskia grandifolia flour), HO (Hypercaloric diet with 5% Opuntia ficus-indicaflour), and HA (Hypercaloric diet with 5% Agave tequilanaflour). Feed intake and apparent digestibility, capillary glycemia, liver weight and Hepato-Somatic Index were evaluated. Adipose tissue mass and the Visceral Fat Index (VFI) and Epididymal Fat Index (EFI) were determined. The data were compared using theTukey test at 5% significance level. There was no statistical difference between the groups for body weight, BMI and Lee Index. The HP and HA groups did not differ from the C group regarding weight gain,the C group had lower apparent digestibility of thediets,the HA group presentedlower blood glucose when compared to the H and HO groups,and the H group had higher liver weight. The HP and HA groups gainedthe lowest adipose tissue mass, VFI and EFI than the H and HO groups. Among the cactaceae studied,Pereskia grandifoliashowed better effects on morphometric and adipose tissue parameters.(AU)

Resumo

To explore the functions of Txnip and its mechanism in adipocyte differentiation, the preadipocytes were isolated from subcutaneous adipose tissue of three-day-old piglets and induced into adipogenic differentiation. The expression of Txnip and ChREBP was silenced, and the Txnip overexpression was achieved in the cells with transfection of the recombinant lentivirus strategies. Txnip silencing promoted the differentiation of porcine preadipocytes and PPARγ expression, and a PPARγ inhibitor reduced this facilitation. Instead, Txnip overexpression exerted a suppressive effect on the cell differentiation and PPARγ expression, and the PPARγ agonist offset this inhibition. High glucose stimulated the preadipocyte differentiation and expressions of ChREBP and Txnip. In contrast, Txnip expression was reduced by ChREBP silencing, suggesting glucose-regulated Txnip expression through the mediation of ChREBP. Moreover, the expressions of ChREBP and Glut4 induced by high glucose and glucose uptake of the cells were reduced by Txnip-overexpression, but increased by Txnip silencing, while these changes of Txnip did not alter their expressions under low glucose. Collectively, Txnip could be an inhibitor of porcine preadipocyte differentiation, which attenuated the adipogenesis through the negative feedback regulation on PPARγ. Txnip impaired the induction of glucose on the preadipocyte differentiation through decreasing Glut4 expression and glucose uptake and subsequent decrease of the expression and transcriptional activity of ChREBP.(AU)

Resumo

Devido ao oferecimento excessivo de alimentos palatáveis e energéticos, em conjunto ao não reconhecimento da afecção como problema, tem desencadeado elevação nos casos de obesidade. Disfunção essa, caracterizada com distribuição epidêmica, responsável por atuar como órgão endócrino, promovendo diversas alterações sobre o organismo. Como consequência da obesidade, ocorre alteração na morfologia do tecido adiposo, gerando desregulação na síntese e liberação de proteínas produzidas pelos adipócitos, denominadas de adipocinas. Algumas dessas substâncias contêm processos anti-inflamatórios, que são diminuídos durante o armazenamento de maior gordura corporal, acarretando em ações localmente ou sistemicamente, gerando como consequência inflamação e desordens metabólicas.

Due to the excessive offering of palatable and energetic foods, together with the failure to recognize the condition as a problem, it has triggered an increase in cases of obesity. This dysfunction, characterized with epidemic distribution, is responsible for acting as an endocrine organ, promoting various changes in the body. As a consequence of obesity, there is a change in the morphology of adipose tissue, generating deregulation in the synthesis and release of proteins produced by adipocytes, called adipokines. Some of these substances contain anti-inflammatory processes, which are reduced during the storage of more body fat, resulting in actions locally or systemically, resulting in inflammation and metabolic disorders.

Resumo

Background: Liposarcoma is a malignant neoplasm of lipoblasts with low incidence in dogs, representing 1.7% of tumorsdiagnosed in the spleen. In veterinary medicine, this neoplasm is classified morphologically into the myxoid, well-differentiated, undifferentiated and pleomorphic subtypes, the latter being one of the most aggressive forms, mainly in cavityorgans. This report refers to a primary splenic pleomorphic liposarcoma in a female dog, addressing anatomopathologicaland immunohistochemical aspects.Case: A 14-year-old, 35 kg bitch mongrel with history of absence of defecation, progressive weight loss, difficulty walking, sensitivity to abdominal palpation, prostration, pale mucous membranes, tachypnea and abdominal distention waspresented to diagnosis. The condition evolved to death and, on necroscopy, there was an increase in splenic volume withneoformation of whitish and reddish color, measuring 32 × 27 cm in its largest axes and weighing 8.9 kg. The neoformationexhibited areas of firm and soft consistency, and sectioning revealed focal areas of extensive necrosis and cavity collectionsof different diameters that allowed the flow of liquid serous contents with a brownish red color. Microscopy showed cellsof neoplastic morphology infiltrating the splenic parenchyma, mostly with slightly acidophilic cytoplasm and few intracytoplasmic lipid vacuoles, which varied in size and distribution. The nucleus of the cells was large, eccentric and irregular,with round to oval morphology, grossly lacy chromatin and single or multiple evident nucleoli. These cells exhibited markedanisocytosis, anisokaryosis and pleomorphism, with more than one mitotic figure per high magnification field visible. Mildinflammatory infiltrate, predominantly lymphocytic, permeated the neoplastic cells, and marked depletion of lymphoidfollicles and atrophy of the red pulp were found in the remaining splenic parenchyma. Immunohistochemical tests...

Resumo

Mortality in piglets during the perinatal period, especially the first days after birth, is frequently caused by non infectious conditions, such as hypoglucemia or low birth weight, which can be associated with hypothermia experienced at birth. The thermal stability of newborn piglets is a fundamental aspect of neonatal care, so maintaining a constant, ideal temperature will substantially reduce newborn mortality. Species-specific characteristics, such as a limited capacity for thermoregulation, low energy reserves, a lack of brown adipose tissue (BAT) (-, and environmental conditions that are adverse for the piglet around the time of birth, including the absence of a microclimate, all of them contribute to difficulties in reaching thermal homeostasis in the first hours post-birth. Shivering thermogenesis and behavioral modifications to regulate body temperature through innate mechanisms allow animals to reduce their energy expenditures. Some body postures are effective in reducing contact with the floor and also nestling are useful to avoid heat loss, and also decreases heat dissipation. Achieving optimal development of thermoregulation is a challenge that newborns must confront to successfully adapt to extrauterine life. The objectives of this review, are to discuss the adverse factors that can lead to a death event due to hypothermia by analyzing the thermoregulation mechanisms at the central and cutaneous levels, also to analyze the harmful impacts that surviving neonate piglets confront in an unfavorable thermal environment, and to describe the pathophysiological mechanisms of death caused by hypothermia.

Resumo

Background: Lipomas are adipocytic tumors of slow and expansive growth. They can be classified into several subtypes depending on the tissue present next to the neoplastic adipocytes. One of such subtypes is the fibrolipoma, which is formed by well-differentiated adipocytes and fibrous connective tissue. This neoplasm has been little described in cattle, and rare reports present the treatment and resolution of the case. Thus, the dissemination of cases of fibrolipomas in cattle is essential to help veterinarians diagnose this neoplasm. The present report describes a case of fibrolipoma in the distal pelvic limb of a cow successfully treated by surgical excision. Case: A 4-year-old 3/4 Girolando cow from the municipality of Vazante, MG, Brazil, was treated on the farm. According to the owner, the animal had been showing tumoral growth in the left pelvic limb over the period of 1 year and 3 months. The tumor involved the entire left metatarsus and was 40 x 37 cm, without ulcerations and painless on palpation. A neoplasm was suspected and surgical excision was the chosen approach. The cow was sedated, positioned and restrained in right lateral recumbency for surgery. The operative field was prepared and a subcutaneous locoregional ring block was performed dorsally to the tumor. The tumor mass was excised with a safety margin of 1 cm. After removal, the mass was found to weigh 10.4 kg and to be yellowish-white upon sectioning. Due to the distance between the edges of the surgical wound, skin suture could not be performed. Thus, second-intention healing and wound protection with bandages were the choice of management. In the postoperative period, the adopted treatment consisted of antibiotic therapy with benzathine penicillin, analgesia with non-steroidal anti-inflammatory drugs, daily dressings and wound protection with bandages. The animal did not show postoperative complications and, over 8 months of monitoring after surgery, there was no recurrence of the neoplasm and the cow was in productive activity. Tumor fragments were fixed in 10% buffered formalin and sent for histopathological examination, which revealed a neoplasm of mesenchymal cells in the subcutaneous adipose tissue supported by dense fibrovascular stroma with solid arrangement. A large amount of dense connective tissue was found among the neoplastic cells. In view of these findings, the diagnosis of fibrolipoma was established. Discussion: Fibrolipoma is a benign neoplasm little described in the veterinary literature, especially in cattle, with only 3 cases reported. To the knowledge of the authors, this is the first report of this neoplasm in the distal limb of cattle. Fibrolipomas are a rare type of lipoma formed by well-differentiated lipocytes and fibrous tissue. The fibrolipoma described in this report was 40 cm in diameter and weighed 10.4 kg, so it can be classified as a type of giant lipoma, as it was more than 10 cm in diameter and more than 1 kg in weight. In the present report and in others in the medical literature, surgical removal of the fibrolipoma resulted in complete recovery of the patient without postoperative complications. Surgical excision is the treatment of choice in these cases and usually promotes healing. However, surgery must be performed early and the neoplasm must be completely removed. The histological characteristics of the fibrolipoma in this case are similar to those found in other cases in cattle and corroborate the choice for surgical treatment and maintenance of the animal in the herd. Although rare, fibrolipomas should be included in the differential diagnosis of tumors of slow and expansive growth in cattle.(AU)

Resumo

Aim: This study aimed to verify the correlation between murine measurements and retroperitoneal adipose tissue in rats exposed to the high-fat diet. Material and methods: Wistar male adult rats, descendants of mothers who consumed a high-fat diet during pregnancy and lactation and fed the same diet after weaning were used. At 60 days of life, body weight, longitudinal axis and waist circumference (WC) were measured. The Body Mass Index (BMI) and the Lee Index were calculated for a posterior analysis of the correlation with the amount of retroperitoneal adipose tissue dissected on the same day. For analysis of the data, the Pearson correlation test was used, considering statistical significance for p<0.05. Results: Body weight had a weak correlation (r= 0.31; p= 0.38) with retroperitoneal adipose tissue. While the longitudinal correlated moderately and negative (r= -0.40; p= 0.25). Abdominal circumference (r= 0.62; p= 0.05), body mass index (r= 0.61; p= 0.03) and Lee (r= 0.69; p= 0.03) correlated moderately and positively with adipose tissue. Conclusion: Among the measured murine measurements, weight and longitudinal axis were not good indicators to represent accumulation of retroperitoneal adipose tissue in rats. However, Lee's index seems to be the best murine marker to diagnose the accumulation of retroperitoneal fat. BMI, CA and Lee index were murine parameters with higher correlation.

Objetivo: Este estudo teve como objetivo verificar a correlação entre medidas murinométricas e tecido adiposo retroperitoneal em ratos expostos à dieta hiperlipídica. Material e métodos: Foram utilizados ratos Wistar machos adultos, descendentes de mães que consumiram dieta hiperlipídica durante a gestação e lactação e alimentados com a mesma dieta após o desmame. Aos 60 dias de vida, foram medidos o peso corporal, o eixo longitudinal e a circunferência da cintura (CC). O Índice de Massa Corporal (IMC) e o Índice de Lee foram calculados para posterior análise da correlação com a quantidade de tecido adiposo retroperitoneal dissecado no mesmo dia. Para análise dos dados, utilizou se o teste de correlação de Pearson, considerando significância estatística para p<0.05. Resultados: O peso corporal apresentou uma correlação fraca (r= 0,31; p= 0,38) com o tecido adiposo retroperitoneal. Enquanto o longitudinal correlacionou moderadamente e negativo (r= -0,40; p= 0,25). A circunferência abdominal (r = 0,62; p = 0,05), índice de massa corporal (r= 0,61; p= 0,03) e Lee (r= 0,69; p= 0,03) correlacionaram-se moderada e positivamente com o tecido adiposo. Conclusão: Entre as medidas murinométricas, o peso e o eixo longitudinal não foram bons indicadores para representar o acúmulo de tecido adiposo retroperitoneal em ratos. No entanto, o índice de Lee parece ser o melhor indicador murinométrico para diagnosticar o acúmulo de gordura retroperitoneal. O IMC, índice de Lee e CA foram parâmetros murinométricos com maior correlação.

Resumo

Abstract Purpose To collect data capable of pointing out the effects of the ultracavitation treatment on the liver of rabbits after adipose tissue application, by means of histological analyses of the liver and hematological and biochemical exams. Methods This is an experimental study with 12 albino rabbits as sample, which were divided into 3 groups and submitted to a hypercaloric diet for one month. Subsequently, subjects underwent UCV treatment: 3 minutes, 30 W, continuous mode at 100%, every 2 ERAS = 441.02 J/cm2, intensity of 10w/cm2. They were then euthanized and underwent biopsy after 24 hours. Results After 48 hours from the ultracavitation treatment, the animals' livers presented greater amount of fat infiltration if compared to the amount presented 96 hours after the treatment. However, laboratory tests showed no alterations. Values were maintained within normal parameters of cholesterol, triglycerides, liver enzymes, hemoglobin and hematocrit levels. Conclusions This study has identified that infiltrates may appear on livers after the treatment, despite high hematological and biochemical tests results. The fat infiltrates reduction 96 h after treatment suggests lower risks to animal health, if the period between applications is respected.

Resumo

The study was carried out to evaluate the effect of AAT-deletion mutation at codon 248 of the BCO2 gene on the content of lutein, ß-carotene, retinol, and α-tocopherol in the liver and fat of crossbred rabbits. The experimental animals comprised 90 rabbits, produced by reciprocal crossing between ins/del heterozygous parents of Flemish Giant, New Zealand Red, and Termond White breeds. All rabbits in the litter were genotyped, given the same diet, and finally slaughtered at 140 d of age. It was found that regardless of the mating scheme employed, all del/del homozygotes had the yellow fat trait, whereas heterozygous and homozygotes animals without the AAT deletion (ins/ins) developed white fat. Lutein concentration in fat was over 13-fold higher in rabbits carrying a homozygous AAT-deletion than in the remaining animals. A codon deletion in the BCO2 gene also contributes to an increase in the concentrations of ß-carotene and α-tocopherol in the adipose tissue of rabbits. However, no differences were observed in the content of the analyzed components between heterozygous and homozygous rabbits without the deletion.(AU)

Resumo

There is a growing interest in the study of unspecialized mesenchymal stem cells, for there are still some discussions about their in vitro behavior. Regenerative medicine is a science undergoing improvement which develops treatments as cell therapy using somatic stem cells. In several studies, adipose tissue is presented as a source of multipotent adult cells that has several advantages over other tissue sources. This study aimed to characterize and evaluate the tagging of mesenchymal stem cells from the agoutis adipose tissue (Dasyprocta prymonolopha), with fluorescent intracytoplasmic nanocrystals. Fibroblast cells were observed, plastic adherent, with extended self-renewal, ability to form colonies, multipotency by differentiation into three lineages, population CD90 + and CD45 - expression, which issued high red fluorescence after the tagging with fluorescent nanocrystals by different paths and cryopreserved for future use. It is possible to conclude that mesenchymal stem cells from agouti adipose tissue have biological characteristics and in vitro behavior that demonstrate its potential for use in clinical tests.(AU)

Há um interesse crescente no estudo das células estaminais mesenquimais, não especializadas, pois ainda existem algumas discussões sobre seu comportamento in vitro. A medicina regenerativa é uma ciência em fase de crescimento que desenvolve tratamentos como terapia celular utilizando células estaminais somáticas. Em vários estudos, o tecido adiposo é apresentado como uma fonte de células adultas multipotentes que tem várias vantagens em relação a outras fontes de tecido. Este estudo teve como objetivo caracterizar e avaliar a marcação de células estaminais mesenquimais do tecido adiposo de cutias (Dasyprocta prymnolopha) com nanocristais intracitoplasmáticos fluorescentes. Observaram-se células fibroblásticas, aderentes ao plástico, com autorrenovação prolongada, capacidade de formar colônias, diferenciação em três linhagens, população CD90 + e expressão CD45, que emitiram alta fluorescência vermelha após a marcação com nanocristais fluorescentes por diferentes vias, e criopreservadas para uso futuro. É possível concluir que as células estaminais mesenquimais do tecido adiposo de cutias têm características biológicas e comportamentos in vitro que demonstram seu potencial para uso em testes clínicos.(AU)

Resumo

The objective of this study was to evaluate carcass yield, measurements, cuts, and fat deposition of sheep fed banana pseudostem hay and Tifton 85 grass hay, with or without virginiamycin. Thirty-three uncastrated male Dorper × Santa Ines crossbred lambs at five months of age, with an average body weight of 25.00 ± 1.95 kg, were used in a feedlot experiment. The following four diets were evaluated: Tifton 85 grass hay plus concentrate with virginiamycin, banana pseudostem hay replacing 60% of Tifton 85 grass hay plus concentrate with virginiamycin, Tifton 85 grass hay plus concentrate without virginiamycin, and banana pseudostem hay replacing 60% of Tifton 85 grass hay plus concentrate without virginiamycin. The experiment was set in a completely randomized design, in a factorial scheme, using orthogonal contrasts. The diet replacing 60% Tifton 85 grass hay by banana pseudostem hay without virginiamycin had no effect on carcass traits. The combination of virginiamycin and Tifton 85 grass hay provided increases in warm carcass weight, warm carcass yield, spine, omental and mesenteric fat deposition, and, regardless of roughage, increases in the thoracic perimeter.

O objetivo deste estudo foi avaliar o rendimento, as medidas, os cortes e a deposição de gordura em ovinos alimentados com feno de pseudocaule de bananeira e feno de capim-tifton 85, com ou sem virginiamicina. Trinta e dois cordeiros mestiços Dorper × Santa Ines, não castrados, aos cinco meses de idade, com peso corporal médio de 25,00 ± 1,95 kg foram utilizados no experimento em regime de confinamento. Foram avaliadas quatro dietas: feno de capim-Tifton 85 mais concentrado com virginiamicina; feno de pseudocaule de bananeira substituindo em 60% o feno de capim Tifton 85 mais concentrado com virginiamicina; feno de capim Tifton 85 mais concentrado sem virginiamicina; e feno de pseudocaule de bananeira substituindo em 60% o feno de capim Tifton 85 mais concentrado sem virginiamicina, em delineamento inteiramente casualizado, em arranjo fatorial 2x2 em contraste ortogonal. A substituição de 60% do feno de capim Tifton 85 por feno de pseudocaule de bananeira sem virginiamicina na dieta de ovinos não altera suas características de carcaça. A virginiamicina associada ao feno de capim Tífton 85 proporcionou aumento do peso de carcaça quente e do rendimento de carcaça quente, aumento do espinhaço, aumento das deposições de gorduras omental e mesentérica e, independente dos volumosos, aumento do perímetro torácico.

Resumo

In veterinary medicine, the cell therapy is still unexplored and there are many unanswered questions that researchers tend to extrapolate to humans in an attempt to treat certain injuries. Investigating this subject in nonhuman primates turns out to be an unparalleled opportunity to better understand the dynamics of stem cells against some diseases. Thus, we aimed to compare the efficiency of bone marrow mononuclear cells (BMMCs) and mesenchymal stem cells (MSCs) from adipose tissue of Chlorocebus aethiops in induced bone injury. Ten animals were used, male adults subjected, to bone injury the iliac crests. The MSCs were isolated by and cultured. In an autologous manner, the BMMCs were infused in the right iliac crest, and MSCs from adipose tissue in the left iliac crest. After 4.8 months, the right iliac crests fully reconstructed, while left iliac crest continued to have obvious bone defects for up to 5.8 months after cell infusion. The best option for treatment of injuries with bone tissue loss in old world primates is to use autologous MSCs from adipose tissue, suggesting we can extrapolate the results to humans, since there is phylogenetic proximity between species.(AU)

Na medicina veterinária, a terapia celular ainda é inexplorada e há muitas perguntas não respondidas, o que leva os pesquisadores a uma tendência a estender a terapia para os seres humanos, na tentativa de tratar certas lesões. Investigar esse assunto em primatas não humanos revela-se uma oportunidade sem precedentes para compreender melhor a dinâmica das células-tronco contra algumas doenças. Assim, objetivou-se comparar a eficiência das células mononucleares de medula óssea (BMMCs) e das células-tronco mesenquimais (MSCs) do tecido adiposo de Chlorocebus aetiops na lesão óssea induzida. Foram utilizados 10 animais, adultos do sexo masculino, submetidos à lesão óssea nas cristas ilíacas. As MSCs foram isoladas e cultivadas; de forma autóloga, as BMMCs foram infundidas na crista ilíaca direita e as MSCs de tecido adiposo na crista ilíaca esquerda. Após 4,8 meses, a crista ilíaca direita foi totalmente reconstruída, enquanto a crista ilíaca esquerda continuou apresentando defeito ósseo evidente por até 5,8 meses após a infusão. A melhor opção para o tratamento de lesões com perda de tecido ósseo em primatas do Velho Mundo é a utilização de MSCs autólogas de tecido adiposo, sugerindo que se podem estender os resultados para seres humanos, uma vez que há proximidade filogenética entre as espécies.(AU)

Resumo

Abstract Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3. Results: Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells. Conclusion: Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages.

Resumo

ABSTRACT: The objective of this research was to examine the effect of subcutaneous fat thickness (SFT) on carcass characteristics and meat quality in 24-month-old British and crossbred British steers finished in feedlot systems. It was assessed using meta-analysis performed on data that was extracted from 46 published studies and comprising a total of 2,695 experimental units. We found that SFT was influenced by and increased linearly with hot carcass weights (HCWs) and cold carcass weights (CCWs) and their yields; moreover, the bone fraction decreased whereas the muscle and fat fractions increased with higher levels of SFT. No significant differences were detected in meat quality variables. Thus, SFT appeared to have a direct influence on HCW, CCW, and the weight and yield of bone, muscle, and fat of castrated steers. A uniform fat thickness level of 6.0 mm SFT was determined to be the standard of quality of carcasses and meat products for consumers.

RESUMO: O objetivo deste trabalho foi avaliar o efeito da espessura de gordura subcutânea (EGS) nas características de carcaça e qualidade de carne em novilhos, com até 24 meses de idade, britânicos ou suas cruzas terminados no sistema de confinamento. Para isso foi utilizado o sistema de meta-análise com dados extraídos de 46 artigos publicados, totalizando 2,695 unidades experimentais. Foi encontrado que a GS influencia no aumento linear no peso de carcaça quente (PCC) e fria (PCF) e em seus rendimentos. Além disso as frações de osso decaem enquanto as frações de músculo e gordura aumentam em altos níveis de GS. Não foi encontrada diferença significativa, mas variáveis de qualidade de carne. Assim a GS apresenta influência direta em PCC, PCF e nos pesos e rendimento de osso, músculo e gordura da carcaça de novilhos castrados. Um valor uniforme de gordura subcutânea de 6,00 mm é capaz de padronizar uma qualidade de carcaça e de produtos aos consumidores.

Resumo

Abstract Aim Obesity during pregnancy is one of the most established risk factors for negative long-term programming. The aim of the present study was to investigate the effects of maternal consumption of a high-fat diet during pregnancy and lactation on the weight gain, visceral adipose tissue and cholesterolemia in neonatal rats. Methods Wistar rats were divided into two groups according to the mother's diet during pregnancy and lactation: Control group (CG, n = 12) were the offspring of rats fed a standard diet (4% lipid) and the Test group (TG, n = 12) were pups rats fed on a high fat diet (23% lipid). The weight of the animals was measured on alternate days until the 22nd day of life, when collected visceral adipose tissue and blood were collected for biochemical analysis. For statistical analysis the Student t test, Sidak´s teste and two way ANOVA was used, with p <0.05. Results the test group showed differences in weight gain, visceral adipose tissue and higher cholesterol. Conclusion a maternal exposure to a high-fat diet during pregnancy and lactation can promote changes in weight gain, hypercholesterolemia and an increase in adipose tissue in neonatal rats.

Resumo Objetivo A obesidade durante a gestação é um dos fatores de risco mais estabelecidos para uma programação negativa em longo prazo. O objetivo do presente estudo foi investigar os efeitos do consumo materno de uma dieta hiperlipídica durante a gestação e lactação no aumento do peso, do tecido adiposo visceral e colesterolemia em ratos neonatos. Métodos Ratos Wistar foram divididos em dois grupos de acordo com a dieta da mãe durante a gestação e lactação: grupo controle (GC, n=12) composto por filhotes de ratas alimentadas com uma dieta padrão (lipídios 4%) e o grupo teste (GT, n=12) composto de filhotes de ratas alimentadas com dieta hiperlipídica (lipídios 23%). O peso dos animais foi aferido em dias alternados até o 22° dia de vida, quando foi coletado sangue para análises bioquímicas. Para a análise estatística utilizou-se os seguintes testes: two way ANOVA, teste de Sidak e teste t de Student, com p< 0,05. Resultados O grupo teste mostrou diferença no ganho de peso, no tecido adiposo visceral e nos níveis de colesterol. Conclusão Uma exposição materna a uma dieta hiperlipídica durante a gestação e lactação pode promover maior ganho ponderal, hipercolesterolemia e um aumento do tecido adiposo em ratos neonatos.

Resumo

Adipose tissue-derived stem cells (ADSCs) are an attractive source of mesenchymal stem cells (MSCs) for use in tissue engineering and clinical applications. This paper focuses on the characterization of ADSCs used as immunosuppressive agent in rabbits undergoing partial allograft for urine bladder restorage. For this study highlighted the characterization of the ADSCs used as immunosuppressive agents in rabbits submitted to partial allograft for restoration of the urinary vesicle, using 25 animals, six months old, New Zealand. ADSCs at the third peal were characterized by the MSC-specific CD105, CD73 and CD90 expression and by the absence of the hematopoietic marker CD45, as revealed by flow cytometry analysis. Moreover, ADSCs were efficient in preventing allograft rejection from the urinary bladder, as judged by biochemical, clinical and ultrasonography analysis. Together, these results compose characterization of protein expression profiles and immunosuppressive functionality of ADSCs in rabbits, which had undergone partial allografts of the urinary bladder, foreseeing future applications in clinical practice.(AU)

As células mesenquimais derivadas de tecido adiposo (ADSCs) são uma fonte atraente de células-tronco mesenquimais (MSCs) para uso na engenharia de tecidos e suas aplicações clínicas. Este trabalho destacou a caracterização das ADSCs utilizadas como agentes imunossupressores em coelhos submetidos a aloenxerto parcial para restauração da vesícula urinária, sendo utilizados 25 animais, de seis meses de idade, Nova Zelândia. As ADSCs, após o terceiro repique, foram caracterizadas pela expressão específica de MSC CD105, CD73 e CD90 e pela ausência do marcador hematopoiético CD45, tal como revelado por análise de citometria de fluxo. Além disso, os ADSCs foram eficientes na prevenção da rejeição de aloenxertos da vesícula urinária, conforme avaliado por análises clínica, bioquímica e ultrassonográfica. Juntos, esses resultados compõem a caracterização dos perfis de expressão proteica e a funcionalidade imunossupressora de ADSCs em coelhos, que sofreram aloenxertos parciais da bexiga, prevendo futuras aplicações na prática clínica.(AU)

Resumo

Células tronco mesenquimais (CTM) são indiferenciadas e multipotentes com capacidade de diferenciação condrogênica e podem sofrer influência de diversos fatores, dentre eles o hormonal. Objetivou-se comparar o efeito da triiodotironina (T3) em diferentes concentrações e tempos, no potencial condrogênico de CTM da medula óssea (CTM-MO) e do tecido adiposo (CTM-TA) de ratas Wistar jovens. Ambas CTM foram extraídas e cultivadas in vitro em meio condrogênico sem T3 (Controles) e com T3 (0,01; 1; 100 e 1000 nM) cada uma, totalizando 10 grupos. Após sete, 14 e 21 dias foram avaliadas expressão gênica de Sox9, Colágeno II (Col II) e Agrecano (Acan) utilizando-se qRT-PCR e formação de matriz condrogênica, por meio de coloração histológica Alcian blue (AB) - ácido periódico de Schiff (PAS). Em Sox9 aumentou a expressão de CTM-MO com T3 100 nM aos 7 dias e T3 1000 nM aos 14 dias, e aumentou a expressão de CTM-TA com T3 1 nM aos 14 dias; em Col II aumentou a expressão de CTM-MO com T3 0,01 nM aos 7 dias e aumentou a expressão de CTMTA com T3 1000 nM aos 14 dias; em Acan aumentou a expressão de CTM-MO com T3 1 nM aos 14 dias e aumentou a expressão de CTM-TA com T3 1 nM aos 21 dias. Com relação à síntese de matriz condrogênica AB-PAS+, aos sete dias de diferenciação a CTM-TA foram superiores à CTM-MO no grupo controle e nas concentrações de T3 0,01 nM, 1 nM e 100 nM, permanecendo semelhante entre as CTM aos 14 e 21 dias. Conclui-se que o potencial condrogênico com síntese de matriz condrogênica da CTM-TA é precocemente superior em relação à CTM-MO aos 7 dias, tanto sem adição de T3 quanto com adição desse hormônio nas concentrações de 0,01 nM, 1 nM e 100 nM.

Mesenchymal stem cells (MSC) are undifferentiated and multipotent, capable of chondrogenic differentiation and may be influenced by several factors, including hormonal. The objective was to compare the effect of triiodothyronine (T3) at different concentrations and times, on the chondrogenic potential of MSC in bone marrow (BMMSC) and adipose tissue (ASC) in young Wistar rats. Both MSCs were extracted and cultivated in vitro in chondrogenic medium without T3 (Controls) and with T3 (0.01; 1; 100 and 1000 nM) each, totaling 10 groups. After seven, 14 and 21 days, gene expression of Sox9, Collagen II (Col II) and Aggrecan (Acan) were evaluated using qRT-PCR and chondrogenic matrix formation, by histological staining Alcian blue (AB) - periodic acid of Schiff (PAS). In Sox9 it increased the expression of BM-MSC with T3 100 nM at 7 days and T3 1000 nM at 14 days, and increased the expression of ASC with T3 1 nM at 14 days; in Col II it increased the expression of BM-MSC with T3 0.01 nM at 7 days and increased the expression of ASC with T3 1000 nM at 14 days; in Acan increased the expression of BM-MSC with T3 1 nM at 14 days and increased the expression of ASC with T3 1 nM at 21 days. Regarding the synthesis of AB-PAS+ chondrogenic matrix, at seven days of differentiation, ASC were superior to BM-MSC in the control group and at concentrations of T3 0.01 nM, 1 nM and 100 nM, remaining similar between MSCs at 14 and 21 days. It is concluded that the chondrogenic potential with chondrogenic matrix synthesis of ASC is precociously superior in relation to BM-MSC at 7 days, both without the addition of T3 and with the addition of this hormone at concentrations of 0.01 nM, 1 nM and 100 nM.

Resumo

Para utilizar as células-tronco mesenquimais caninas (CTMs) em terapias é imprescindível que sejam formados criobancos de armazenamento celular, sendo assim, a metodologia da criopreservação de CTMs necessita de estudos, uma vez que o efeito da congelação e dos agentes crioprotetores sobre as células obtidas de tecido adiposo canino ainda é pouco conhecido. Desta forma, o objetivo deste estudo foi avaliar os efeitos de diferentes tipos de soluções na criopreservação das CTMs, sobre a estrutura, viabilidade, proliferação celular, ultraestrutura e expressão gênica, bem como no seu desenvolvimento pós-descongelação. Para isto, foram isoladas células-tronco de 12 cadelas. As amostras foram divididas em 5 tratamentos: não criopreservado (controle) = NCRIO; 90% SFB (Soro fetal Bovino) com 10% DMSO = SFB; Meio de cultivo DMEM/F12 alta glicose acrescido com 20% SFB e 10% DMSO = DMEM/F12; DMEM/F12 alta glicose, acrescido com 5%PVA, 5% de ITS, 5% de BSA e 10% DMSO = PVA/ITS/BSA e BambankerTM = BAM. Sendo que em todos foram utilizados o Dimetilsufóxido (DMSO) como agente crioprotetor principal. As células foram avaliadas antes da criopreservação, pós-descongelação imediata e após o recultivo. Foi realizada uma análise de custo das diferentes soluções. A viabilidade e apoptose celular foram avaliadas pelo teste de exclusão com o corante Trypan blue e anexina V; a proliferação celular, pela curva de crescimento; a ultraestrutura em microscópio eletrônico de transmissão, a expressão gênica por RT-qPCR e a análise de custo através de pesquisa de mercado. Todos os dados foram submetidos a análise estatística pelo software SAS versão 9.4. Os resultados mostraram que as CTMs criopreservadas com o meio comercial foi superior aos demais quanto ao número de células e a proliferação celular, mas foi similar aos grupos NCRIO e PVA/ITS/BSA quanto a viabilidade celular. Embora não tenham sido observadas alterações morfológicas significativas entre os tratamentos de criopreservação, a ultraestrutura celular variou de acordo com os componentes do meio, no qual a morfologia mais próxima da encontrada nas células frescas foi observada nos grupos PVA/ITS/BSA e BAM. A expressão gênica demonstrou que as células apresentaram diferenças quanto aos tratamentos para os transcritos CD44, TGFB1, CXCL12 e IDO. Neste estudo foi observado que embora o tratamento com a marca comercial tenha sido superior aos demais crioprotetores, as outras combinações de substâncias crioprotetoras testadas foram eficientes na criopreservação das CTMs caninas, sendo alternativas que podem ser utilizadas. Todos os tratamentos possibilitaram a retomada das funções celulares normais após a descongelação e recultivo. E as soluções autoformuladas são economicamente mais viáveis que a marca comercial. Esses resultados são importantes para o estabelecimento de protocolos de criopreservação de CTMs nessa espécie, pois aumentam as possibilidades de escolha da solução crioprotetora e também permite o uso de soluções com custos mais acessíveis e sem componentes contaminantes como o SFB.

In order to use canine mesenchymal stem cells (MSCs) in therapies, it is essential that cryobanks of cell storage are formed, therefore, the methodology of cryopreservation of MSCs needs studies, since the effect of freezing and cryoprotective agents on the cells obtained from canine adipose tissue is still poorly understood. Thus, the aim of this study was to evaluate the effects of different types of solutions in the cryopreservation of MSCs, on the structure, viability, cell proliferation, ultrastructure and gene expression, as well as on their post-thawing development. For this, stem cells from 12 female dog were isolated. The samples were divided into 5 treatments: not cryopreserved (control) = NCRIO; 90% FBS (Fetal Bovine Serum) with 10% DMSO = FBS; High glucose DMEM/F12 culture medium plus 20% FBS and 10% DMSO = DMEM/F12; DMEM/F12 high glucose plus 5%PVA, 5% ITS, 5% BSA and 10% DMSO = PVA/ITS/BSA and BambankerTM = BAM. In all, Dimethylsuffoxide (DMSO) was used as the main cryoprotective agent. Cells were evaluated before cryopreservation, immediate post-thawing and after reculture. A cost analysis of the different solutions was carried out. Cell viability and apoptosis were evaluated by the Trypan blue dye and annexin V exclusion test; cell proliferation, by the growth curve; ultrastructure in transmission electron microscope, gene expression by RT-qPCR and cost analysis through market research. All data were submitted to statistical analysis using the SAS software version 9.4. The results showed that the MSCs cryopreserved with the commercial medium was superior to the others in terms of cell number and cell proliferation, but was similar to the NCRIO and PVA/ITS/BSA groups in terms of cell viability. Although no significant morphological changes were observed between the cryopreservation treatments, the cellular ultrastructure varied according to the components of the medium, in which the morphology closest to that found in fresh cells was observed in the PVA/ITS/BSA and BAM groups. The gene expression showed that the cells showed differences regarding the treatments for the CD44, TGFB1, CXCL12 and IDO transcripts. In this study, it was observed that although the treatment with the commercial brand was superior to other cryoprotectants, the other combinations of cryoprotective substances tested were efficient in the cryopreservation of canine MSCs, being alternatives that can be used. All treatments allowed the resumption of normal cell functions after thawing and reculture. And self-formulated solutions are more economically viable than branded. These results are important for the establishment of cryopreservation protocols for MSCs in this species, as they increase the possibilities of choosing the cryoprotectant solution and also allow the use of more affordable solutions without contaminating components such as FBS.

Resumo

The objective was to evaluate the in vitro effect of prolactin in osteogenic potential of adipose tissue-derived mesenchymal stem cells (ADSCs) in female rats. ADSCs were cultured in osteogenic medium with and without the addition of prolactin and distributed into three groups: 1) ADSCs (control), 2) ADSCs with addition of 100ng/mL of prolactin and 3) ADSCs with addition of 300ng/mL of prolactin. At 21 days of differentiation, the tests of MTT conversion into formazan crystals, percentage of mineralized nodules and cells per field and quantification of genic transcript for alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I by real-time RT-PCR were made. The addition of prolactin reduced the conversion of MTT in group 3 and increased the percentage of cells per field in the groups 2 and 3, however without significantly increasing the percentage of mineralized nodules and the expression of alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I. In conclusion, the addition of prolactin in concentrations of 100ng/mL and 300ng/mL does not change the osteogenic differentiation to the ADSCs of female rats despite increase in the cellularity of the culture.(AU)

O objetivo do presente trabalho foi avaliar o efeito in vitro da prolactina sobre o potencial osteogênico de células-tronco mesenquimais do tecido adiposo (CTM-TA) em ratas. CTM-TA foram cultivadas em meio osteogênico com e sem adição de prolactina e distribuídas em três grupos: 1) CTM-TA (controle), 2) CM-TA com adição de 100ng/mL de prolactina e 3) CTM-TA com adição de 300ng/mL de prolactina. Aos 21 dias de diferenciação, foram realizados os testes de conversão do MTT em cristais de formazan, porcentagem de nódulos mineralizados e células por campo e quantificação dos transcritos gênicos para fosfatase alcalina, osteopontina, osteocalcina, sialoproteína óssea, BMP-2 e colágeno I. A adição de prolactina reduziu a conversão do MTT no grupo 3 e aumentou a porcentagem de células por campo nos grupos 2 e 3, sem alterar significativamente a porcentagem de nódulos mineralizados e a expressão de fosfatase alcalina, osteopontina, osteocalcina, sialoproteína óssea, BMP-2 e colágeno I. Conclui-se que a adição de prolactina nas concentrações de 100ng/mL e 300ng/mL não altera a diferenciação osteogênica das CTM-TA de ratas, apesar do aumento de celularidade da cultura.(AU)

Resumo

Abstract Aim Obesity during pregnancy is one of the most established risk factors for negative long-term programming. The aim of the present study was to investigate the effects of maternal consumption of a high-fat diet during pregnancy and lactation on the weight gain, visceral adipose tissue and cholesterolemia in neonatal rats. Methods Wistar rats were divided into two groups according to the mother's diet during pregnancy and lactation: Control group (CG, n = 12) were the offspring of rats fed a standard diet (4% lipid) and the Test group (TG, n = 12) were pups rats fed on a high fat diet (23% lipid). The weight of the animals was measured on alternate days until the 22nd day of life, when collected visceral adipose tissue and blood were collected for biochemical analysis. For statistical analysis the Student t test, Sidak´s teste and two way ANOVA was used, with p 0.05. Results the test group showed differences in weight gain, visceral adipose tissue and higher cholesterol. Conclusion a maternal exposure to a high-fat diet during pregnancy and lactation can promote changes in weight gain, hypercholesterolemia and an increase in adipose tissue in neonatal rats.

Resumo Objetivo A obesidade durante a gestação é um dos fatores de risco mais estabelecidos para uma programação negativa em longo prazo. O objetivo do presente estudo foi investigar os efeitos do consumo materno de uma dieta hiperlipídica durante a gestação e lactação no aumento do peso, do tecido adiposo visceral e colesterolemia em ratos neonatos. Métodos Ratos Wistar foram divididos em dois grupos de acordo com a dieta da mãe durante a gestação e lactação: grupo controle (GC, n=12) composto por filhotes de ratas alimentadas com uma dieta padrão (lipídios 4%) e o grupo teste (GT, n=12) composto de filhotes de ratas alimentadas com dieta hiperlipídica (lipídios 23%). O peso dos animais foi aferido em dias alternados até o 22° dia de vida, quando foi coletado sangue para análises bioquímicas. Para a análise estatística utilizou-se os seguintes testes: two way ANOVA, teste de Sidak e teste t de Student, com p 0,05. Resultados O grupo teste mostrou diferença no ganho de peso, no tecido adiposo visceral e nos níveis de colesterol. Conclusão Uma exposição materna a uma dieta hiperlipídica durante a gestação e lactação pode promover maior ganho ponderal, hipercolesterolemia e um aumento do tecido adiposo em ratos neonatos.