Resumo
This study investigated oxidative damage and exocrine dysfunction of fetal pancreas caused by maternal nutritional restriction. Eighteen ewes carrying singleton fetus were randomly divided into control group (CG, ad libitum, 0.67 MJ ME/BW0.75/d, n = 6), restricted group 1 (RG1, 0.33 MJ ME/BW0.75/d, n = 6), and restricted group 2 (RG2, 0.18 MJ ME/BW0.75/d, n = 6) at d 90 of pregnancy. Maternal undernutrition was imposed from d 90 to 140 of pregnancy. At 140 d of gestation, fetal blood and pancreas tissue were collected to determine fetal pancreatic extracellular matrix, antioxidant capacity, and indicators of exocrine dysfunction. With the decrease of maternal nutrition, the fetal body weight, pancreatic weight, and DNA content were reduced in RG2 compared with CG, and increased and thickened collagen fibers were observed in RG2. Fetuses in RG2 exhibited increased collagen 3 (COL3) and fibronectin (FN) levels relative to CG, and the COL1:COL3 ratio was lower than that of the CG. For RG1, we found increased COL3 compared with CG. Malondialdehyde, serum amylase, and serum lipase in fetal pancreas in RG2 increased, but the total antioxidant capacity (T-AOC) decreased compared with the CG. The impaired ovine fetal pancreas growth, antioxidant imbalance, and pancreatic exocrine dysfunction are induced by maternal undernutrition during late pregnancy.
Assuntos
Animais , Doenças dos Ovinos , Estresse Oxidativo , Desnutrição/veterinária , Pâncreas Exócrino/anormalidades , Feto/anormalidadesResumo
Maternal colostrum provides the neonate with immunoglobulins, essential for passive immunity. However, storage and handling of colostrum can alter its physical and nutritional characteristics. The aim of this study was to evaluate the effects of three conservation methods on colostrum density, pH, total antioxidant capacity (TAC), protein (PP) and lipid peroxidation (LP). Colostrum samples were collected from 15 Bos indicus cows, and divided into three aliquots for storage using three methods: refrigeration at 4°C, freezing at -20°C, and lyophilization. For each method, four evaluation times were performed: day (d) 5, 10, 15, and 20 for refrigeration and d 15, 30, 45, and 60 for freezing and lyophilization. pH and density analyses were performed at each evaluation time for each method. On d 0 and 15 of storage, TAC, LP, and PP analyses were performed. A generalized linear model, Tukey's test for means comparisons, and a Pearson correlation analysis were conducted. A decrease in refrigerated colostrum pH was observed on d 15. Density of the lyophilized colostrum decreased, as compared with that of frozen colostrum. Lyophilization exhibited the lower PP values of samples, whereas refrigeration presented the highest values of LP and PP. No differences in colostrum TAC were observed between storage methods. A positive correlation between PP and colostrum density and a negative correlation between colostrum density and TAC were found. It was concluded that both freezing and lyophilization are suitable storage methods for bovine colostrum, as they limit proteins and lipids oxidation, and maintain the TAC of fresh colostrum.
O colostro materno fornece ao recém-nascido imunoglobulinas que são essenciais para a imunidade passiva. No entanto, o armazenamento e o manuseio do colostro podem alterar suas características físicas e nutricionais. O objetivo deste estudo foi avaliar os efeitos de três métodos de conservação na densidade, pH, capacidade antioxidante total (CAT), peroxidação protéica (PP) e lipídica (LP) do colostro. Amostras de colostro foram coletadas de 15 vacas Bos indicus, e divididas em três alíquotas para armazenamento usando três métodos: refrigeração a 4 °C, congelamento a -20 °C e liofilização. Em cada método, foram realizados quatro tempos de avaliação: dia (d) 5, 10, 15 e 20 para refrigeração e dia 15, 30, 45 e 60 para congelamento e liofilização. Além disso, as análises de pH e densidade foram realizadas em cada momento de avaliação para cada método. No dia 0 e 15 de conservação foram realizadas análises da CAT, PL e PP. Foi realizado um modelo linear generalizado, teste de Tukey para comparação de médias e análise de correlação de Pearson. Uma diminuição no pH do colostro refrigerado foi encontrada no dia 15. A densidade do colostro liofilizado diminuiu em comparação com o colostro congelado. A liofilização apresentou menor PP das amostras, enquanto a refrigeração apresentou os maiores valores de PL e PP. Não foram observadas diferenças no TAC do colostro entre os métodos de armazenamento. Foi encontrada uma correlação positiva entre PP e densidade do colostro e uma correlação negativa entre densidade do colostro e CAT. Assim, concluiu-se que tanto o congelamento quanto a liofilização são métodos adequados de armazenamento do colostro bovino, pois limitam a oxidação de proteínas e lipídios e mantêm o TAC do colostro fresco.
Assuntos
Animais , Feminino , Bovinos , Bovinos , Colostro/química , Armazenamento de Alimentos/métodos , Oxidação/análise , Alimentos Resfriados , Liofilização , CongelamentoResumo
This study was conducted to investigate effects of vitamin A (VA) and vitamin K3 (VK3) on immune function and intestinal antioxidant capacity of aged laying hens. In a 3 × 3 factorial arrangement, the diets of 1080 Roman Pink laying hens (87 weeks old) was formulated with deficient, adequate and excess VA and VK3, including 0, 7000 and 14000 IU/kg VA and 0, 2.0 and 4.0 mg/kg VK3 for 8 weeks. Interactive effects between VA and VK3 were observed that VA and VK3 decreased the splenetic mRNA expression of inducible nitric oxide synthase (iNOS) and tumour necrosis factor α (TNF-α), but increased the plasma immunoglobulin G (IgG) content and jejunal mRNA expression of nuclear factor-like 2 (Nrf2). Hens fed adequate or excess VA had higher spleen index, mRNA expression of interleukin-10 (IL-10) in spleen, sIgA content, catalase (CAT), glutathione peroxidase and total dismutase (T-SOD) activity, and mRNA expression of polymeric immunoglobulin receptor (pIgR) in jejunum and lower mRNA expression of IL-1ß in jejunum and iNOS, TNF-α in spleen. Furthermore, adequate or excess VK3 significantly increased plasma IgG content, the CAT, T-SOD and total antioxidant capacity activities, up-regulated the mRNA expression of pIgR, Nrf2, SOD1 and CAT in jejunum and down-regulated the mRNA expression of iNOS and TNF-α in spleen.(AU)
Assuntos
Vitamina A/efeitos adversos , Galinhas/imunologia , Vitamina K 3/efeitos adversos , Sistema Imunitário , Antioxidantes/análiseResumo
In Rio Grande do Sul, the main rice producer State in Brazil, low temperatures can occur during germination and seedling establishment, and in some cases, during the reproductive stage. When low temperatures occur in the early developmental stages cause delay in germination, resulting in a non-homogeneous growing. In reproductive stage, low temperatures cause spikelet sterility, directly interfering with plant yield. Researchers have shown that some phenolic compounds such as proanthocyanidins and anthocyanin are associated with low temperature tolerance in plants due to their antioxidant capacity. The red and black color in the seeds of some rice genotypes is conferred by the phenolic compounds proanthocyanidins and anthocyanin, respectively. Therefore, tis study aimed to verify whether rice genotypes with red or black seeds are more tolerant to low temperatures during germination. In this study, five rice genotypes were tested, two present seeds without pigmentation and with contrasting response to low temperature tolerance (BRS Bojuru - tolerant and BRS Pampeira - sensitive), two genotypes with red seeds (BRS 902, SCS 119 Rubi) and one black seed genotype (SCS 120 Ônix). As expected, the genotypes with pigmented seeds had a greater total phenolic compounds content. However, under low temperature conditions, the genotypes with pigmented seed showed a similar response to the sensitive genotype. Therefore, the presence of proanthocyanidins and anthocyanin in the seed of the studied genotypes does not provide tolerance to low temperatures during germination.(AU)
No Rio Grande do Sul, principal estado produtor de arroz do Brasil, baixas temperaturas podem ocorrer durante a germinação e estabelecimento de plântulas, e em alguns casos, durante o estádio reprodutivo. Quando baixas temperaturas ocorrem no estádio inicial de desenvolvimento causam atraso da germinação, resultando em um crescimento não homogêneo. No estádio reprodutivo, baixas temperaturas podem ocasionar esterilidade das espiguetas, interferindo diretamente na produtividade da planta. Pesquisas têm mostrado que alguns compostos fenólicos como as proantocianidinas e antocianinas estão associadas com tolerância a baixa temperatura em plantas devido sua capacidade antioxidante. A coloração vermelha e preta nas sementes de alguns genótipos de arroz é conferida pelos compostos fenólicos proantocianidinas e antocianinas, respectivamente. Portanto, o objetivo desse estudo foi verificar se os genótipos de arroz com sementes vermelhas ou pretas são mais tolerantes a baixas temperaturas durante a germinação. Neste trabalho, cinco genótipos foram testados, dois com sementes sem pigmentação e com resposta contrastante para tolerância a baixa temperatura (BRS Bojuru - tolerante e BRS Pampeira - sensível), dois genótipos com sementes vermelhas (BRS 902, SCS 119 Rubi) e um genótipo com sementes pretas (SCS 120 Ônix). Como esperado, os genótipos com sementes pigmentadas têm maior conteúdo de compostos fenólicos totais. Entretanto, sob condições de baixa temperatura, os genótipos com semente pigmentada mostraram resposta similar ao genótipo sensível. Dessa forma, a presença de proantocianidinas e antocianinas nas sementes dos genótipos estudados não confere tolerância a baixas temperaturas durante a germinação.(AU)
Assuntos
Oryza/fisiologia , Flavonoides/efeitos adversos , Germinação/efeitos dos fármacos , Temperatura Baixa/efeitos adversos , Resposta ao Choque Frio/fisiologiaResumo
Biofortification of food crops is implemented through the application of mineral fertilizers, to improve the levels of essential mineral elements for human nutrition. Magnesium is a key macronutrient in crop production and quality; however, worldwide, it is the most limiting macronutrient in agriculture. Magnesium plays an important role in manipulating physiological and biochemical processes in plants. Therefore, the objective of this study was to evaluate the efficacy of biofortification with magnesium chloride and sulfate on the accumulation of bioactive compounds and antioxidant capacity in snap bean cv. Strike. Two sources of Magnesium were applied via edaphic route: Magnesium chloride and magnesium sulfate at doses of 0, 50, 100 and 200 ppm during 2018 in Mexico. Accumulation of bioactive compounds (total phenols, total flavonoids and total anthocyanins) and antioxidant capacity in snap bean fruits were evaluated. Results obtained indicate that the concentration of total flavonoids, total anthocyanins and antioxidant capacity in the edible parts of snap beans were higher in the MgSO4 treatment than in the MgCl2 treatments, exceeding 30, 59 and 6% respectively. This is one of the first studies on the edaphic agronomic biofortification of Mg+ and its effect on nutraceutical quality in snap bean. An interesting result is that MgSO4 produced high concentrations of anthocyanins in edible snap beans. These results can be applied as a new strategy to reduce malnutrition and improve the health of the population in poor urban and rural communities in developing countries.(AU)
A biofortificação de culturas alimentares é implementada através da aplicação de fertilizantes minerais, para melhorar os níveis de elementos minerais essenciais para a nutrição humana. O magnésio é um macronutriente essencial na produção e qualidade das culturas, no entanto, em todo o mundo, é o macronutriente mais limitante da agricultura. O magnésio desempenha um papel importante na manipulação de processos fisiológicos e bioquímicos nas plantas. Portanto, o objetivo deste estudo foi avaliar a eficácia da biofortificação com cloreto e sulfato de magnésio no acúmulo de compostos bioativos e capacidade antioxidante em feijões verdes cv. Strike. Duas fontes de magnésio foram aplicadas por via edáfica: cloreto de magnésio e sulfato de magnésio nas doses de 0, 50, 100 e 200 ppm durante o ano de 2018 no México. Foram avaliados o acúmulo de compostos bioativos (fenóis totais, flavonóides totais e antocianinas totais) e a capacidade antioxidante em frutos de feijão-vagem. Os resultados obtidos indicam que a concentração de flavonoides totais, antocianinas totais e capacidade antioxidante nas partes comestíveis do feijão-vagem foram maiores no tratamento com MgSO4 do que nos tratamentos com MgCl2, ultrapassando 30, 59 e 6% respectivamente. Este é um dos primeiros estudos sobre a biofortificação agronômica edáfica do Mg+ e seu efeito na qualidade nutracêutica do feijão-vagem. Um resultado interessante é que o MgSO4 produziu altas concentrações de antocianinas no feijão-vagem comestível. Esses resultados podem ser aplicados como uma nova estratégia para reduzir a desnutrição e melhorar a saúde da população em comunidades urbanas e rurais pobres em países em desenvolvimento.(AU)
Assuntos
Fabaceae/química , Magnésio/administração & dosagem , Antocianinas/biossíntese , Flavonoides/análise , Proteínas de Vegetais ComestíveisResumo
The effect of high hydrostatic pressure (HHP) application on whey protein concentrate was evaluated both before (pre-treatment - PT) and during (hydrolysis assisted - HA) hydrolysis processes. A factorial design 22 with 3 central points was used with pressure (100, 250, 400 MPa) and time (5, 20 and 35 minutes) as independent variables. The hydrolysis was evaluated and monitored by soluble protein, aromatic amino acid contents and RP-HPLC. ABTS and ORAC tests were used to evaluate the in vitro antioxidant capacity. The reduction of soluble protein content was approximately 20% for conventional hydrolysis and for all PT treatments up to 4 h of reaction, while HHP assisted hydrolysis at 100 MPa showed a 35% protein reduction after 35 minutes of reaction. In addition, pressurization favored peptic hydrolysis of -lactoglobulin by up to 98% and also improved the in vitro antioxidant capacity of the hydrolysates, which increased from 34.25 to 60.89 moles TE g-1 of protein in the best treatment. The results suggest that the use of HHP assisted hydrolysis favored the peptic hydrolysis, with a reduction in hydrolysis time and increased antioxidant activity.(AU)
Neste estudo, o efeito da aplicação de alta pressão hidrostática (HHP) sobre o concentrado proteico de soro de leite foi avaliado antes (pré-tratamento - PT) e durante os processos de hidrólise (assistida por hidrólise - HA). Utilizou-se o delineamento fatorial 22 com três pontos centrais, onde as variáveis independentes foram pressão (100, 250, 400 MPa) e tempo (5, 20 e 35 minutos). A hidrólise foi avaliada pelo conteúdo de proteínas solúveis e aminoácidos aromáticos, além do perfil peptídico por RP-HPLC. As análises de ABTS e ORAC foram utilizadas para avaliar a capacidade antioxidante in vitro. A redução do teor de proteína solúvel foi de aproximadamente 20% para a hidrólise convencional e para todos os pontos de PT até 4h de reação, enquanto a hidrólise assistida por HHP a 100 MPa mostrou uma redução de 35% de proteína em 35 minutos de reação. Além disso, a pressurização favoreceu a hidrólise péptica da -lactoglobulina em até 98% e também melhorou a capacidade antioxidante in vitro dos hidrolisados, que aumentaram de 34,25 para 60,89 moles de TE g-1 de proteína no melhor tratamento. Os resultados sugerem que o uso da hidrólise assistida por HHP favoreceu a hidrólise péptica, com redução no tempo de hidrólise e aumento da atividade antioxidante.(AU)
Assuntos
Proteínas do Soro do Leite/antagonistas & inibidores , Proteínas do Soro do Leite/análise , Enzimas/farmacocinética , Especificidade por SubstratoResumo
Medicinal plants have a significant role in preventing and curing several diseases, and Tanacetum L. is one of these plants. The aim of the present study is to determine the fatty acid, lipid-soluble vitamin, sterol, phenolic content, and antioxidant capacity of Tanacetum densum subsp. laxum and Tanacetum densum subsp. amani, to compare the effect of altitude on the biochemical content and to compare systematically by using fatty acids and phenolics. This study showed that palmitic acid (C16:0) and stearic acid (C18:0) are major sources of saturated fatty acid and oleic acid (C18:1 n9), and linoleic acid (18:2 n6c) and a-linolenic acid (C18:3 n3) are the principal unsaturated fatty acids in the two endemic Tanacetum densum taxa. Also, this study found that the unsaturated fatty acid content (60.11±1.61%) of Tanacetum densum subsp. laxum was higher than the unsaturated fatty acid content (44.13±1.28%) of Tanacetum densum subsp. amani. And also, the 6/3 ratio of Tanacetum densum subsp. laxum (1.74) and Tanacetum densum subsp. amani (1.60) was found to be similar. However, this study determined that the lipid soluble vitamin and sterol content of two endemic Tanacetum taxa are low except for stigmasterol. Present study showed that catechin is principal phenolic in the Tanacetum densum taxa. This study also found that Tanacetum densum subsp. laxum and Tanacetum densum subsp. amani had the highest levels of catechin, vanillic acid, and caffeic acid content though the phenolic amounts, particularly catechin and quercetin, were dissimilar in the T. densum taxa. This study suggested that ecological conditions such as altitude may affect the biochemical content of two endemic Tanacetum densum taxa. Furthermore, the current study determined that two endemic Tanacetum L. taxa had potent radical scavenging capacities and found a correlation between total phenolics and antioxidant activity.(AU)
As plantas medicinais têm um papel significativo na prevenção e cura de várias doenças, e Tanacetum L. é uma dessas plantas. O objetivo do presente estudo é determinar o conteúdo de ácido graxo, vitamina lipossolúvel, esterol, estrutura fenólica e capacidade antioxidante de Tanacetum densum subsp. laxum e Tanacetum densum subsp. amani, comparar o efeito da altitude sobre o conteúdo bioquímico e realizar uma comparação sistemática usando ácidos graxos e fenólicos. Esta pesquisa mostrou que o ácido palmítico (C16:0) e o ácido esteárico (C18:0) são as principais fontes de ácido graxo saturado e que o ácido oleico (C18:1 n9), o ácido linoleico (18:2 n6c) e o ácido a-linolênico (C18: 3 n3) são os principais ácidos graxos insaturados nos dois táxons endêmicos de Tanacetum densum. Além disso, este estudo descobriu que o conteúdo de ácidos graxos insaturados (60,11±1,61%) de Tanacetum densum subsp. laxum foi superior ao conteúdo de ácidos graxos insaturados (44,13±1,28%) de Tanacetum densum subsp. amani, e também que a razão 6/3 de Tanacetum densum subsp. laxum (1,74) e Tanacetum densum subsp. amani (1,60) foi semelhante. No entanto, este trabalho determinou que o conteúdo de vitamina lipossolúvel e esterol de dois táxons endêmicos de Tanacetum é baixo, exceto o estigmasterol, além de descobrir que Tanacetum densum subsp. laxum e Tanacetum densum subsp. amani apresentaram os mais altos níveis de conteúdo de catequina, ácido vanílico e ácido cafeico, embora as quantidades fenólicas, especialmente catequina e quercetina, sejam diferentes nos táxons de T. densum. Este estudo sugere que condições ecológicas, como a altitude, podem afetar o conteúdo bioquímico de dois táxons endêmicos de Tanacetum densum. Ainda, esta pesquisa determinou que dois táxons de Tanacetum L. endêmicos possuíam potentes capacidades de sequestro de radicais e que houve correlação entre fenólicos totais e atividade antioxidante.(AU)
Assuntos
Asteraceae/química , Fenômenos Bioquímicos , Antioxidantes/análise , Ácidos Graxos , Vitaminas Lipossolúveis/análise , FitosteróisResumo
The aim of this study was to assess the effect of selenium-enriched Candida utilis with high contents of organic selenium (Se) and glutathione (GSH) on growth performance, antioxidant capacity and immune function of broiler chickens. A total of 100 healthy 7-day-old male broiler chickens were randomly divided into 5 groups, and fed diets supplemented with (a) Na2SeO3, (b) C. utilis, (c) Se-enriched Saccharomyces cerevisiae, (d) Se-enriched C. utilis, and (e) the control without any supplements. The experiment lasted for 6 weeks and parameters were recorded on day 42. No significant differences in average daily gain were found among the 5 groups. However, Se-enriched C. utilis supplemented in the diet increased activities of glutathione peroxidase in the whole blood (p 0.01), catalase in the serum (p 0.01) and breast meat (p 0.01), and superoxide dismutase in the breast meat (p 0.01), as well as decreased contents of malondialdehyde in the serum (p 0.01), liver (p 0.01) and breast meat (p 0.05). Also, Se-enriched C. utilis improved titers of IgG (p 0.01), IgM (p 0.01), and IgA (p 0.01) in the serum, as compared to the control. All these results indicated that Se-enriched C. utilis was a good candidate of dietary supplement to improve the antioxidant capacity and immune function of broiler chickens.(AU)
Assuntos
Animais , Galinhas , Antioxidantes , Candida , SelênioResumo
The aim of this study was to assess the effect of selenium-enriched Candida utilis with high contents of organic selenium (Se) and glutathione (GSH) on growth performance, antioxidant capacity and immune function of broiler chickens. A total of 100 healthy 7-day-old male broiler chickens were randomly divided into 5 groups, and fed diets supplemented with (a) Na2SeO3, (b) C. utilis, (c) Se-enriched Saccharomyces cerevisiae, (d) Se-enriched C. utilis, and (e) the control without any supplements. The experiment lasted for 6 weeks and parameters were recorded on day 42. No significant differences in average daily gain were found among the 5 groups. However, Se-enriched C. utilis supplemented in the diet increased activities of glutathione peroxidase in the whole blood (p 0.01), catalase in the serum (p 0.01) and breast meat (p 0.01), and superoxide dismutase in the breast meat (p 0.01), as well as decreased contents of malondialdehyde in the serum (p 0.01), liver (p 0.01) and breast meat (p 0.05). Also, Se-enriched C. utilis improved titers of IgG (p 0.01), IgM (p 0.01), and IgA (p 0.01) in the serum, as compared to the control. All these results indicated that Se-enriched C. utilis was a good candidate of dietary supplement to improve the antioxidant capacity and immune function of broiler chickens.
Assuntos
Animais , Antioxidantes , Candida , Galinhas , SelênioResumo
The present study aimed to develop a mixed smoothie drink using the solid albumen of the green coconut in its composition. Smoothie formulations were prepared following an experimental design, setting the solid albumen concentration at 20% and varying the contents of the acerola (Barbados Cherry), pineapple, and coconut water pulps, which corresponded to 80% of the total mass of the product. Response parameters evaluated were vitamin C content, antioxidant capacity, and overall sensory acceptance. Ten formulations were evaluated. The ones that contained higher concentrations of acerola pulp had higher values of bioactive compounds but were not the most sensorially accepted. By means of the desirability function, a final formulation consisted of 52.8% of pineapple, 27.2% of acerola, and 20.0% of solid albumen of green coconut. This new formulation was well accepted, with a grade (score) 7 (good). It represents a good nutritional contribution and a source of vitamin C which can contribute to add value to a co-product of the beverage industry.(AU)
O objetivo deste trabalho foi desenvolver uma bebida mista do tipo smoothie utilizando o albúmen sólido do coco verde na sua composição. As formulações do smoothie foram elaboradas seguindo um planejamento experimental, fixando-se a concentração do albúmen sólido em 20% e variando-se os teores das polpas de acerola, abacaxi e água de coco, que totalizaram 80% da massa total do produto. Os parâmetros de resposta avaliados foram o teor de vitamina C, a capacidade antioxidante e a aceitação sensorial global. Foi observado que das dez formulações avaliadas, as que continham maiores concentrações de polpa de acerola, apresentaram valores superiores de compostos bioativos, porém não foram as mais aceitas sensorialmente. Por meio da função desejabilidade chegou-se a uma formulação final que consistiu de 52,8% de abacaxi, 27,2% de acerola e 20,0% de albúmen sólido de coco verde. Esta nova formulação teve boa aceitação, com nota igual a 7 (bom) e representa um bom aporte nutricional, sendo fonte de vitamina C, podendo contribuir para agregar valor a um coproduto da indústria de bebidas.(AU)
Resumo
A doença periodontal (DP) é a enfermidade inflamatória mais comum da cavidade oral dos cães. A quantificação de biomarcadores do plasma e da saliva tem sido utilizada para avaliar o estresse oxidativo sistêmico (EOS) e local (EOL) da DP humana. Na DP canina, os mecanismos do estresse oxidativo não estão bem caracterizados e estabelecidos. O objetivo do presente estudo foi investigar a hipótese de que o EOS ocorre na DP canina e de que a saliva pode ser utilizada para avaliar o EOL. Analisou-se, também, a hipótese de que a ativação do metabolismo oxidativo dos neutrófilos contribui para EOS na DP dos cães. Para tal, foram selecionados 20 cães adultos portadores de DP, agrupados de acordo com o grau de lesão: gengivite (n=6), periodontites leve (n=8) e avançada (n=6). O grupo controle foi composto pelos mesmos 20 cães, 30 dias após o tratamento periodontal. Para avaliar o metabolismo oxidativo dos neutrófilos circulantes foi quantificada a produção de superóxido pelo teste de redução do tetrazólio nitroazul (NBT). As concentrações de oxidante total (TOC) e de espécies reativas ao ácido tiobartbitúrico (TBARS) no plasma foram quantificadas para avaliar o EOS. Para a avaliação do estresse oxidativo local, foi quantificado o TOC salivar e a concentração dos principais antioxidantes da saliva (albumina, ácido úrico e bilirrubina total). O EOS na DP foi confirmado pelo aumento da produção de superóxido dos neutrófilos circulantes, TOC e TBARS plasmático. Foi possível quantificar todos os biomarcadores na saliva de cães, porém nenhum foi capaz de expressar o EOL da DP canina. Esta é uma das primeiras evidências de que o EOS ocorre em cães com DP e que a ativação do metabolismo oxidativo dos neutrófilos pode contribuir para desequilíbrio entre antioxidantes e oxidantes. Este estudo ressalta a importância da higiene bucal dos cães para a prevenção da DP e de lesões degenerativas crônicas de diversos tecidos causadas pelo EOS.(AU)
Periodontal disease (PD) is the most common inflammatory disease of the oral cavity of dogs. Quantitation of plasma and salivary biomarkers have been used to assess the systemic oxidative stress (SOS) and local (LOS) of human PD. In canine PD, oxidative stress mechanisms are not well characterized and established. Our objective was to investigate the hypothesis that SOS occurs in dog PD and saliva can be used to evaluate the LOS. We also investigated the hypothesis that the activation of neutrophil oxidative metabolism contributes to SOS in dog SD. For this purpose, 20 adult dogs were selected PD patients, grouped according to the degree of injury: gingivitis (n=6), light periodontitis (n=8) and advanced periodontitis (n=6). The control group was composed of the same 20 dogs, 30 days after periodontal treatment. To assess oxidative metabolism of circulating neutrophils superoxide production was measured by test nitroblue tetrazolium reduction (NBT). The total oxidant concentrations (TOC) and reactive species to tiobartbitúrico acid (TBARS) in plasma were quantified to evaluate SOS. For the evaluation of local oxidative stress were quantified salivary TOC and concentration of the main antioxidant in saliva (albumin, uric acid, and total bilirubin). EOS in dogs with PD was confirmed by increased superoxide production of circulating neutrophils, TOC, and plasma TBARS. It was possible to quantify all the biomarkers in the saliva of dogs, but none was able to express the LOS canine PD. This is the first evidence that SOS occurs in dogs with PD and that activation of the oxidative metabolism of neutrophils may contribute to an imbalance between oxidants and antioxidants. This study highlights the importance of oral hygiene of dogs to prevent PD and chronic degenerative lesions of various tissues caused by SOS.(AU)
Assuntos
Animais , Cães , Biomarcadores/análise , Cães/anatomia & histologia , Estresse Oxidativo , Periodontite/veterináriaResumo
A doença periodontal (DP) é a enfermidade inflamatória mais comum da cavidade oral dos cães. A quantificação de biomarcadores do plasma e da saliva tem sido utilizada para avaliar o estresse oxidativo sistêmico (EOS) e local (EOL) da DP humana. Na DP canina, os mecanismos do estresse oxidativo não estão bem caracterizados e estabelecidos. O objetivo do presente estudo foi investigar a hipótese de que o EOS ocorre na DP canina e de que a saliva pode ser utilizada para avaliar o EOL. Analisou-se, também, a hipótese de que a ativação do metabolismo oxidativo dos neutrófilos contribui para EOS na DP dos cães. Para tal, foram selecionados 20 cães adultos portadores de DP, agrupados de acordo com o grau de lesão: gengivite (n=6), periodontites leve (n=8) e avançada (n=6). O grupo controle foi composto pelos mesmos 20 cães, 30 dias após o tratamento periodontal. Para avaliar o metabolismo oxidativo dos neutrófilos circulantes foi quantificada a produção de superóxido pelo teste de redução do tetrazólio nitroazul (NBT). As concentrações de oxidante total (TOC) e de espécies reativas ao ácido tiobartbitúrico (TBARS) no plasma foram quantificadas para avaliar o EOS. Para a avaliação do estresse oxidativo local, foi quantificado o TOC salivar e a concentração dos principais antioxidantes da saliva (albumina, ácido úrico e bilirrubina total). O EOS na DP foi confirmado pelo aumento da produção de superóxido dos neutrófilos circulantes, TOC e TBARS plasmático. Foi possível quantificar todos os biomarcadores na saliva de cães, porém nenhum foi capaz de expressar o EOL da DP canina. Esta é uma das primeiras evidências de que o EOS ocorre em cães com DP e que a ativação do metabolismo oxidativo dos neutrófilos pode contribuir para desequilíbrio entre antioxidantes e oxidantes. Este estudo ressalta a importância da higiene bucal dos cães para a prevenção da DP e de lesões degenerativas crônicas de diversos tecidos causadas pelo EOS.(AU)
Periodontal disease (PD) is the most common inflammatory disease of the oral cavity of dogs. Quantitation of plasma and salivary biomarkers have been used to assess the systemic oxidative stress (SOS) and local (LOS) of human PD. In canine PD, oxidative stress mechanisms are not well characterized and established. Our objective was to investigate the hypothesis that SOS occurs in dog PD and saliva can be used to evaluate the LOS. We also investigated the hypothesis that the activation of neutrophil oxidative metabolism contributes to SOS in dog SD. For this purpose, 20 adult dogs were selected PD patients, grouped according to the degree of injury: gingivitis (n=6), light periodontitis (n=8) and advanced periodontitis (n=6). The control group was composed of the same 20 dogs, 30 days after periodontal treatment. To assess oxidative metabolism of circulating neutrophils superoxide production was measured by test nitroblue tetrazolium reduction (NBT). The total oxidant concentrations (TOC) and reactive species to tiobartbitúrico acid (TBARS) in plasma were quantified to evaluate SOS. For the evaluation of local oxidative stress were quantified salivary TOC and concentration of the main antioxidant in saliva (albumin, uric acid, and total bilirubin). EOS in dogs with PD was confirmed by increased superoxide production of circulating neutrophils, TOC, and plasma TBARS. It was possible to quantify all the biomarkers in the saliva of dogs, but none was able to express the LOS canine PD. This is the first evidence that SOS occurs in dogs with PD and that activation of the oxidative metabolism of neutrophils may contribute to an imbalance between oxidants and antioxidants. This study highlights the importance of oral hygiene of dogs to prevent PD and chronic degenerative lesions of various tissues caused by SOS.(AU)
Assuntos
Animais , Cães , Biomarcadores/análise , Cães/anatomia & histologia , Estresse Oxidativo , Periodontite/veterináriaResumo
The current study was conducted to determine the effects of the licorice root (Glycyrrhriza glabra) in laying hens diets on performance, egg cholesterol, some plasma parameters and antioxidant capacity. One hundred, 40-week old laying hens were divided into four groups, each group consisted of 25 hens and were placed in individual cages. The mean of the initial body weight of laying hens was 1829.18±9.595 g. Commercial laying hen diet was supplemented with 0, 0.5, 1.0 and 2.0% levels of licorice root powder and four different dietary groups were formed. From the experimental findings, it was ascertained that the licorice root supplementation had no significant effect on egg weight and feed conversion ratio (p>0.05), but feed consumption decreased with increasing licorice root (p<0.05).Egg yield was recorded as 88.94%, 89.56%, 86.82% and 85.02% in the groups of 0, 0.5, 1.0 and 2.0, respectively (p<0.05).Plasma low density lipoprotein (LDL) and egg yolk cholesterol level decreased with the addition of licorice root, while plasma high density lipoprotein (HDL) level was increased with licorice root addition (p<0.05). Licorice root addition had a positive effect on total antioxidant capacity (TAS) of plasma. It was determined that the total antioxidant capacity was increased by increasing amount of licorice root. From the overall findings, it can be concluded that licorice root could be used as a feed additive without any adverse effect on performance. It has been demonstrated that the licorice root enables the production of functional eggs.(AU)
Assuntos
Animais , Feminino , Glycyrrhiza/efeitos adversos , Galinhas/sangue , Galinhas/fisiologia , Ovos/análise , Colesterol/análise , Plasma/química , Antioxidantes , Oviparidade , Alimentos Fortificados/efeitos adversosResumo
The current study was conducted to determine the effects of the licorice root (Glycyrrhriza glabra) in laying hens diets on performance, egg cholesterol, some plasma parameters and antioxidant capacity. One hundred, 40-week old laying hens were divided into four groups, each group consisted of 25 hens and were placed in individual cages. The mean of the initial body weight of laying hens was 1829.18±9.595 g. Commercial laying hen diet was supplemented with 0, 0.5, 1.0 and 2.0% levels of licorice root powder and four different dietary groups were formed. From the experimental findings, it was ascertained that the licorice root supplementation had no significant effect on egg weight and feed conversion ratio (p>0.05), but feed consumption decreased with increasing licorice root (p<0.05).Egg yield was recorded as 88.94%, 89.56%, 86.82% and 85.02% in the groups of 0, 0.5, 1.0 and 2.0, respectively (p<0.05).Plasma low density lipoprotein (LDL) and egg yolk cholesterol level decreased with the addition of licorice root, while plasma high density lipoprotein (HDL) level was increased with licorice root addition (p<0.05). Licorice root addition had a positive effect on total antioxidant capacity (TAS) of plasma. It was determined that the total antioxidant capacity was increased by increasing amount of licorice root. From the overall findings, it can be concluded that licorice root could be used as a feed additive without any adverse effect on performance. It has been demonstrated that the licorice root enables the production of functional eggs.
Assuntos
Feminino , Animais , Colesterol/análise , Galinhas/fisiologia , Galinhas/sangue , Glycyrrhiza/efeitos adversos , Ovos/análise , Plasma/química , Alimentos Fortificados/efeitos adversos , Antioxidantes , OviparidadeResumo
The high intensity ultrasound-assisted extraction (HIU) is one of the most simple, quick and efficient techniques for the extraction of phenolic and other antioxidant compounds from plants. This is the first application of HIU for the extraction of these compounds from goldenberry fruit. The HIU and conventional extraction techniques showed similar results regarding to phenolic compounds and antioxidant capacity. However, the time required for HIU extraction (5min) was 24 times lower than conventional extraction (120min). Phenolic compounds reported were chlorogenic acid, caffeic acid and rutin. In vitro cytotoxicity assays were used for evaluation of extracts and the results showed that in a wide range of concentration, the extract maintains cell viability, thus indicating the possibility to use it as food with safety.(AU)
A extração assistida com ultrassom de alta intensidade (HIU) é uma das técnicas mais simples, rápidas e eficientes na extração de compostos fenólicos e antioxidantes de plantas. Este trabalho foi o primeiro a utilizar HIU na extração destes compostos presentes na fruta goldenberry. As técnicas HIU e extração convencional apresentaram resultados semelhantes com relação aos compostos fenólicos e capacidade antioxidante. Entretanto, o tempo necessário na HIU (5min) foi 24 vezes menor que na extração convencional (120min). Os compostos fenólicos encontrados foram ácido clorogênico, ácido cafeico e rutina. Ensaios de citotoxicidade in vitro foram usados para avaliação dos extratos e os resultados demonstraram que, em ampla faixa de concentração, o extrato mantém a viabilidade celular, indicando assim possível segurança para utilização em alimentos.(AU)
Assuntos
Plantas Tóxicas/química , Plantas Tóxicas/toxicidade , Ultrassom/métodos , Compostos Fenólicos , Physalis/toxicidade , Antioxidantes/análise , Cromatografia Líquida de Alta PressãoResumo
Background: Respiratory disease negatively affects the physiological performance of racehorses. Inflammatory Airway Disease (IAD) is a typical respiratory disease that affects young horses. The pathogenesis of IAD remains incompletely defined but it is thought to be associated with the inhalation of irritants such as barn dust and air pollution. Although noninfectious agents are likely to be central to the development of IAD, infectious agents can be associated with the disease. The main objective of the present study was to determine the relationships between IAD and antioxidant capacity, ceruloplasmin levels and bacterial and fungal infection status, in racehorses. Materials, Methods & Results: In this study, 25 thoroughbred race horses with exercise intolerance and respiratory system symptoms were evaluated as the study group and 10 healthy horses were included in the control group. Clinical examinations, endoscopic evaluation and tracheal lavage were administered to all horses. Mucus accumulation in the trachea was scored and the horses having a mucus score of ≥ 3 were included the study group. Tracheal lavage fluids were investigated to determine the microbiological status of all animals. In addition, blood samples were collected and total oxidant capacity (TOC), total antioxidant capacity (TAC) and ceruloplasmin (Cp) levels were determined with a spectroscopic method. Exercise intolerance and various respiratory system symptoms such as coughing, nasal discharge and noisy breathing, were the common findings from horses in the study group. Fungal culture was negative and only Streptococcus equi subsp. zooepidemicus strain was isolated from 6 samples. No statistical difference was determined between the TOC, TAC and Cp levels of the groups (P > .05).[...]
Assuntos
Animais , Antioxidantes/análise , Cavalos , Ceruloplasmina/análise , Doenças Respiratórias/veterinária , Infecções Bacterianas/veterinária , Micoses/veterináriaResumo
Background: Respiratory disease negatively affects the physiological performance of racehorses. Inflammatory Airway Disease (IAD) is a typical respiratory disease that affects young horses. The pathogenesis of IAD remains incompletely defined but it is thought to be associated with the inhalation of irritants such as barn dust and air pollution. Although noninfectious agents are likely to be central to the development of IAD, infectious agents can be associated with the disease. The main objective of the present study was to determine the relationships between IAD and antioxidant capacity, ceruloplasmin levels and bacterial and fungal infection status, in racehorses. Materials, Methods & Results: In this study, 25 thoroughbred race horses with exercise intolerance and respiratory system symptoms were evaluated as the study group and 10 healthy horses were included in the control group. Clinical examinations, endoscopic evaluation and tracheal lavage were administered to all horses. Mucus accumulation in the trachea was scored and the horses having a mucus score of ≥ 3 were included the study group. Tracheal lavage fluids were investigated to determine the microbiological status of all animals. In addition, blood samples were collected and total oxidant capacity (TOC), total antioxidant capacity (TAC) and ceruloplasmin (Cp) levels were determined with a spectroscopic method. Exercise intolerance and various respiratory system symptoms such as coughing, nasal discharge and noisy breathing, were the common findings from horses in the study group. Fungal culture was negative and only Streptococcus equi subsp. zooepidemicus strain was isolated from 6 samples. No statistical difference was determined between the TOC, TAC and Cp levels of the groups (P > .05).[...](AU)
Assuntos
Animais , Ceruloplasmina/análise , Cavalos , Doenças Respiratórias/veterinária , Antioxidantes/análise , Infecções Bacterianas/veterinária , Micoses/veterináriaResumo
The present study aimed at evaluating the effect of two strains of probiotics (Bacillus subtilis PB6 and Bacillus Cereus var. toyoi) on selected indicators of the antioxidant status and immune responses of broilers before and after challenge with Salmonella enterica subsp. Typhimurium (S. typhimurium). Birds were distributed into the following five treatments: negative control (CTL); negative control+S. typhimurium (CTL+); antibiotic+S. typhimurium, (AB); Toyocerin(r)+S. typhimurium (TOYO); and CloSTATTM+ S. typhimurium (CLOS). Birds in all treatments, except for CTL, were orally challenged with 3 x109 CFUS. typhimurium at 16 days of age. The results showed that total antioxidant capacity (TAC), superoxide dismutase activity (SOD), and hydrogen peroxide (H2O2) values were similar among all groups. The antibody titers against S. typhimurium and Newcastle disease (ND) were not affected by the treatments (p>0.05). However, S. typhimurium and ND titers were influenced by the interaction between week and treatment (p 0.01, p 0.05, respectively) and by week (p 0.001, p 0.001, respectively). In conclusion, S. typhimurium challenge had an impact on anti-Salmonella and NC titers. Both probiotics did not affect the broilers' antioxidant capacity.
Assuntos
Animais , Antioxidantes/metabolismo , Antioxidantes/química , Galinhas/genética , Salmonella enterica/isolamento & purificação , Estresse Oxidativo/genética , Probióticos/análise , Probióticos/efeitos adversosResumo
The present study aimed at evaluating the effect of two strains of probiotics (Bacillus subtilis PB6 and Bacillus Cereus var. toyoi) on selected indicators of the antioxidant status and immune responses of broilers before and after challenge with Salmonella enterica subsp. Typhimurium (S. typhimurium). Birds were distributed into the following five treatments: negative control (CTL); negative control+S. typhimurium (CTL+); antibiotic+S. typhimurium, (AB); Toyocerin(r)+S. typhimurium (TOYO); and CloSTATTM+ S. typhimurium (CLOS). Birds in all treatments, except for CTL, were orally challenged with 3 x109 CFUS. typhimurium at 16 days of age. The results showed that total antioxidant capacity (TAC), superoxide dismutase activity (SOD), and hydrogen peroxide (H2O2) values were similar among all groups. The antibody titers against S. typhimurium and Newcastle disease (ND) were not affected by the treatments (p>0.05). However, S. typhimurium and ND titers were influenced by the interaction between week and treatment (p 0.01, p 0.05, respectively) and by week (p 0.001, p 0.001, respectively). In conclusion, S. typhimurium challenge had an impact on anti-Salmonella and NC titers. Both probiotics did not affect the broilers' antioxidant capacity.(AU)
Assuntos
Animais , Galinhas/genética , Antioxidantes/química , Antioxidantes/metabolismo , Salmonella enterica/isolamento & purificação , Probióticos/efeitos adversos , Probióticos/análise , Estresse Oxidativo/genéticaResumo
O uso de alimentos ricos em compostos bioativos, como carotenóides, flavonóides e vitaminas, são capazes de melhorar as respostas bioquímicas e fisiológicas dos organismos criados em cativeiro e consequentemente aumentam sua resiliência quando confrontados a diferentes situações de estresse. A polpa da fruta Mauritia flexuosa amazônica, palmeira conhecida como buriti, é um alimento rico em ácidos graxos insaturados, carotenoides, flavonóides e ácido ascórbico, entre outros compostos bioativos. Dessa maneira, o objetivo deste trabalho de Tese foi avaliar os efeitos do uso da polpa do fruto desta planta na dieta do camarão Litopenaeus vannamei, considerando sua capacidade de prevenir a geração de estresse oxidativo, influência na coloração, conteúdo de carotenoides no músculo e hepatopâncreas, respostas do sistema antioxidante, peroxidação lipídica e características da textura do músculo pós-morte, conservado sob congelamento e ao logo do tempo de estocagem. Foram preparadas dietas com níveis crescentes de inclusão da polpa do fruto da M. flexuosa amazônica (0, 1,25, 2,50, 5,00 e 10,00 % nos capítulos I, II e III; e 0, 5,00 e 10,00% no capítulo IV), que se ofertou a pós-larvas (cap. II e III, peso médio inicial de 5 ± 0,3 mg), a juvenis (cap. I, peso médio inicial 1,26 ± 0,023 g) e indivíduos adultos (cap. IV, peso médio inicial de 8,87 ± 0,64 g) do camarão L. vannamei. CAPÍTULO I: Não foi observado diferença significativa (p > 0,05) para os parâmetros de crescimento, cromaticidade no músculo, conteúdo de flavonóides nos tecidos analisados. No entanto, os resultados de carotenóides total mostrou que o uso da polpa do fruto da M. flexuosa foi capaz de aumentar significativamente os níveis de carotenóides total no hepatopâncreas e músculo dos camarões (p < 0,05), sendo evidenciada uma correlação significativa com a melhora da capacidade antioxidante nesses tecidos (R2 = 0.99 e R2 = 0.97, respectivamente; p < 0,05) e diminuição da peroxidação lipídica no XII hepatopâncreas (R2 = 0.97; p < 0,05). A análise de custo mostrou um decréscimo dos valores gastos, quando se utilizou 5,00 e 10,00% de M. flexuosa (8.11% e 7.24% de kg de ração por kg de peso ganho; e 18.76% e 12.22% de kg de ração por kg de proteína ganho, respectivamente). CAPÍTULO II: Não foi observada diferença no crescimento das pós-larvas (p > 0,05). No entanto, tanto o conteúdo de carotenóides total como a capacidade antioxidante das pós-larvas melhorou com a inclusão da M. flexuosa nas dietas (apresentando um valor ótimo de inclusão da polpa do fruto da M. flexuosa de 7,67% e 5,88%, respectivamente) (p < 0,05). Da mesma forma, os níveis de peroxidação lipídica foram menores com 5,00 e 10,00% de inclusão da polpa do fruto da M. flexuosa (sendo o valor de 9,17% onde se obteve a menor nível de peroxidação lipídica) (p <0,05). Um melhor custo foi obtido com a inclusão de 5% (10,50 e 10,63% de redução de custo para ganho de peso e proteína, respectivamente). CAPÍTULO III: A capacidade antioxidante foi maior nos camarões alimentados com 2,50 e 5,00% de inclusão da polpa do fruto da M. flexuosa e expostos a toxidade por amônia (p < 0,05). Já quando expostos a toxidade por nitrito, a capacidade antioxidante foi maior com a inclusão de 5,00 e 10,00% da polpa do fruto da M. flexuosa (p < 0,05). Da mesma forma, os camarões alimentados com 2,50, 5,00 e 10,00% de inclusão da polpa do fruto da M. flexuosa tiveram uma diminuição nos níveis de peroxidação lipídica. No entanto, não houve diferença nos níveis de glutationa reduzida e grupos sulfidrilas associados a proteínas (p > 0,05). CAPÍTULO IV: Os dados da conservação mostraram que a inclusão da polpa do fruto da M. flexuosa influenciou o aumento de carotenóides total no músculo dos camarões (p < 0,05). Sendo evidenciada uma diminuído o dano lipídico e a preservação da textura do músculo, dos camarões alimentados com inclusão da M. flexuosa e conservados cru. O valor de bases nitrogenadas voláteis total foi menor nos camarões alimentados com 5,00% de inclusão da M. flexuosa. Destaca-se com este XIII estudo que a polpa do fruto da M. flexuosa é um importante 51 insumo, rico em compostos bioativos, que favorece a capacidade do camarão L. vannamei de enfrentar os desafios associados à produção intensiva e sua conservação pós-abate, ainda apresentando redução no custo de produção.
The use of foods rich in bioactive compounds, such as carotenoids, flavonoids and vitamins, are able to improve the biochemical and physiological responses of organisms raised in captivity and increase their resilience when faced with different stress situations. The Amazonian Mauritia flexuosa fruit pulp, palm tree known as buriti, is food rich in unsaturated fatty acids, carotenoids, flavonoids and ascorbic acid, among other bioactive compounds. Thus, the objective of work this Thesis was to evaluate the effects of using the pulp of this fruit in the diet of the shrimp Litopenaeus vannamei considering its ability to prevent the generation of oxidative stress, influence on color, content of carotenoids in the muscle and hepatopancreas, responses of the antioxidant system, lipid peroxidation and characteristics of the texture of the postmortem muscle, preserved under freezing and during storage time. A diet was prepared with increasing levels of inclusion of the Amazonian M. flexuosa fruit pulp (0,1.25, 2.50, 5.00 and 10.00% in chapters I, II and III; and 0, 5.00 and 10.00% in Chapter IV), which was offered to post-larvae (chap. II and III, initial mean weight of 5 ± 0.3mg), to juveniles (chap. I, initial mean weight 1.26 ± 0.023 g) and adult individuals (cap. IV, initial average weight of 8.87 ± 0.64 g) of L. vannamei shrimp. CHAPTER I: There was no significant difference (p > 0.05) for growth parameters, muscle chromaticity, flavonoid content in the analyzed tissues. However, the results of total carotenoids showed that the use of the M. flexuosa fruit pulp was able to significantly increase the levels of total carotenoids in the hepatopancreas and shrimp muscle (p <0.05), being evidenced a significant correlation with the improvement of antioxidant capacity in these tissues (R2 = 0.99 and R2 80 = 0.97, respectively; p <0.05) and decrease in lipid peroxidation in the hepatopancreas (R2 81 = 0.97; p <0.05). The cost analysis showed a decrease in the amounts spent, when 5.00 and 10.00% of M. flexuosa fruit pulp were XV used (8.11% and 7.24% of kg of feed per kg of weight gained; and 18.76% and 12.22% of kg of feed per kg of protein gained, respectively). CHAPTER II: There was no difference in the growth of post-larvae (p > 0.05). However, both the total carotenoid content and the antioxidant capacity of the post-larvae improved with the inclusion of M. flexuosa fruit pulp in the diets (presenting an optimum inclusion value of the M. flexuosa fruit pulp of 7.67% and 5, 88%, respectively) (p < 0.05). In the same way, the levels of lipid peroxidation were lower with 5.00 and 10.00% of M. flexuosa fruit pulp inclusion (the value being 9.17% where the lowest level of lipid peroxidation was obtained) (p <0.05). A better cost was obtained with the inclusion of 5.00% (10.50 and 10.63% cost reduction for weight and protein gain, respectively). CHAPTER III: The antioxidant capacity was higher in shrimp fed 2.50 and 5.00% of the M. flexuosa fruit pulp inclusion and exposed to toxicity by ammonia (p < 0.05). When exposed to nitrite toxicity, the antioxidant capacity was greater with 5.00 and 10.00% of the M. flexuosa fruit pulp inclusion (p < 0.05). In the same way, the shrimp fed 2.50, 5.00 and 10.00% of the M. flexuosa fruit pulp inclusion had a decrease in the levels of lipid peroxidation. However, there was no difference in the levels of reduced glutathione and sulfhydryl groups associated with proteins (p > 0.05). CHAPTER IV: Conservation data showed that M. flexuosa fruit pulp inclusion influenced the increase in total carotenoids in the shrimp muscle (p < 0.05). With a decrease in lipid damage and preservation of muscle texture, shrimps fed with M. flexuosa fruit pulp inclusion and preserved raw were evidenced. The value of total volatile nitrogenous bases was lower in prawns fed with 5.00% of the M. flexuosa fruit pulp inclusion. It is evidenced with this study that M. flexuosa fruit pulp is an important input, rich in bioactive compounds, which favors the capacity of L. vannamei to face the challenges associated with intensive production and XVI its post-mortem conservation, still presenting financial feasibility 107 to reduction cost production.