Resumo
The aim of this research was to evaluate the effects of supplementation of maturation medium withcaptopril in oocyte in vitro maturation (IVM). 470 CCOs were recovered and classified into grades I and II,being divided into four groups: G1 (n = 56), that was control group; G2 (n = 152) 20 mM of captopril; G3 (n =126) 40 uM of captopril; and G4 (n = 136) 80 uM of captopril, then these were subsequently submitted to IVMprocess for 24 hours at 38.5°C in 5% CO2. After 24 hours of maturation, oocytes were denuded and evaluatedfor the first polar body extrusion and were, therefore, considered matured. The addition of the Captopril inmedium of oocytes maturation has not improved the IVM rate.(AU)
Assuntos
Animais , Feminino , Bovinos , Captopril/química , Técnicas de Maturação in Vitro de Oócitos/instrumentação , Técnicas de Maturação in Vitro de Oócitos/veterinária , AngiotensinasResumo
Objective to evaluate the effect of captopril in vitro production of bovine embryos. 472 COCs were usedfrom abattoir ovaries, which were screened, sorted and selected in grades I and II, and submitted to the PIVprocess. The IVM treatments were added in the following concentrations: T1 - control; T2 - 5 mM of captopril;T3 - 10 uM of captopril; and T4 - 15 captopril uM, the COCs were subjected to IVM, IVF CIV. The results showthat there was no statistically significant difference (P > 0.05) between treatments during maturation. Thevariables studied were cleavage and blastocyst rates. The captopril supplementation did not improve (P > 0.05)G1 cleavage rate - 61.84%; G2 - 71.00%; G3 - 68.87%; and G4 - 56.90%. Supplementation of 20μM ofcaptopril influenced the amount of viable embryos.(AU)
Assuntos
Animais , Bovinos , Captopril/efeitos adversos , Captopril/análise , Bovinos/embriologia , Embrião de Mamíferos/citologiaResumo
This study aimed to evaluate the effect of captopril on the in vitro maturation of oocytes. 1627 COCscattle slaughter houses were used, were subsequently screened, classified into grades I and II, according to themorphological quality, and submitted to the IVM process, distributed in four treatments: T1 - control; T2 - 5μMof captopril; T3 - 10μM of captopril; and T4 - 15μM of captopril, incubated in an incubator at 38.5 ° C with anatmosphere of 5% CO2. After 22 hours, the COCs were stripped to evaluate the rate of maturation, which wereas follows: T1 - 56.60% (120/212); T2 - 60.60% (240/396); T3 - 52.88% (174/329) and T4 - 58.30% (207/355).The results show that there was no statistically significant difference ( P> 0.05) between treatments duringmaturation, concluding that captopril supplementation did not affect the maturation of COCs.(AU)
Assuntos
Animais , Feminino , Bovinos , Captopril/efeitos adversos , Técnicas de Maturação in Vitro de Oócitos/métodos , Técnicas de Maturação in Vitro de Oócitos/veterinária , Bovinos/embriologiaResumo
The aim of this study was to evaluate the effects of supplementation of maturation medium withcaptopril, and its consequences in the bovine maturation, fertilization and embryo development in vitro. 326bovine ovaries from a local slaughterhouse were used. 1101 CCOs were recovered and distributed in fourgroups: G1 (n = 112), that was control group; G2 (n = 322) 20μM of captopril; G3 (n = 367) 40μM ofcaptopril; and G4 (n = 300) captopril 80μM and later submitted to IVM. The matured CCOs were fertilized, andco-incubated. After fertilization, 676 presumptive zygotes were cultured and maintained in the greenhouse for 7days. The total number of viable embryos was 12; 39; 32 and 31, respectively in the experimental groups.Considering the experimental conditions adopted, it was concluded that the addition of the Captopril in mediumof oocytes IVM positively doesnt influence embryonic development, as evidenced by the similar percentages ofembryo production.(AU)
Assuntos
Animais , Bovinos , Desenvolvimento Embrionário , Bovinos/crescimento & desenvolvimento , Captopril/administração & dosagem , Captopril/químicaResumo
Objective to evaluate the effect of captopril in vitro production of bovine embryos. 472 COCs were usedfrom abattoir ovaries, which were screened, sorted and selected in grades I and II, and submitted to the PIVprocess. The IVM treatments were added in the following concentrations: T1 - control; T2 - 5 mM of captopril;T3 - 10 uM of captopril; and T4 - 15 captopril uM, the COCs were subjected to IVM, IVF CIV. The results showthat there was no statistically significant difference (P > 0.05) between treatments during maturation. Thevariables studied were cleavage and blastocyst rates. The captopril supplementation did not improve (P > 0.05)G1 cleavage rate - 61.84%; G2 - 71.00%; G3 - 68.87%; and G4 - 56.90%. Supplementation of 20μM ofcaptopril influenced the amount of viable embryos.
Assuntos
Animais , Bovinos , Bovinos/embriologia , Captopril/análise , Captopril/efeitos adversos , Embrião de Mamíferos/citologiaResumo
This study aimed to evaluate the effect of captopril on the in vitro maturation of oocytes. 1627 COCscattle slaughter houses were used, were subsequently screened, classified into grades I and II, according to themorphological quality, and submitted to the IVM process, distributed in four treatments: T1 - control; T2 - 5μMof captopril; T3 - 10μM of captopril; and T4 - 15μM of captopril, incubated in an incubator at 38.5 ° C with anatmosphere of 5% CO2. After 22 hours, the COCs were stripped to evaluate the rate of maturation, which wereas follows: T1 - 56.60% (120/212); T2 - 60.60% (240/396); T3 - 52.88% (174/329) and T4 - 58.30% (207/355).The results show that there was no statistically significant difference ( P> 0.05) between treatments duringmaturation, concluding that captopril supplementation did not affect the maturation of COCs.
Assuntos
Feminino , Animais , Bovinos , Bovinos/embriologia , Captopril/efeitos adversos , Técnicas de Maturação in Vitro de Oócitos/métodos , Técnicas de Maturação in Vitro de Oócitos/veterináriaResumo
The aim of this research was to evaluate the effects of supplementation of maturation medium withcaptopril in oocyte in vitro maturation (IVM). 470 CCOs were recovered and classified into grades I and II,being divided into four groups: G1 (n = 56), that was control group; G2 (n = 152) 20 mM of captopril; G3 (n =126) 40 uM of captopril; and G4 (n = 136) 80 uM of captopril, then these were subsequently submitted to IVMprocess for 24 hours at 38.5°C in 5% CO2. After 24 hours of maturation, oocytes were denuded and evaluatedfor the first polar body extrusion and were, therefore, considered matured. The addition of the Captopril inmedium of oocytes maturation has not improved the IVM rate.
Assuntos
Feminino , Animais , Bovinos , Angiotensinas , Captopril/química , Técnicas de Maturação in Vitro de Oócitos/instrumentação , Técnicas de Maturação in Vitro de Oócitos/veterináriaResumo
The aim of this study was to evaluate the effects of supplementation of maturation medium withcaptopril, and its consequences in the bovine maturation, fertilization and embryo development in vitro. 326bovine ovaries from a local slaughterhouse were used. 1101 CCOs were recovered and distributed in fourgroups: G1 (n = 112), that was control group; G2 (n = 322) 20μM of captopril; G3 (n = 367) 40μM ofcaptopril; and G4 (n = 300) captopril 80μM and later submitted to IVM. The matured CCOs were fertilized, andco-incubated. After fertilization, 676 presumptive zygotes were cultured and maintained in the greenhouse for 7days. The total number of viable embryos was 12; 39; 32 and 31, respectively in the experimental groups.Considering the experimental conditions adopted, it was concluded that the addition of the Captopril in mediumof oocytes IVM positively doesnt influence embryonic development, as evidenced by the similar percentages ofembryo production.