Resumo
Sows do not produce enough milk to sustain optimal growth of their litters. This is particularly important when considering the current hyperprolific sow genetic lines. Mammary development needs to be considered to improve potential milk yield. One can only attempt to stimulate mammogenesis during periods when rapid mammary development is already ongoing. There are two such periods before lactation starts, namely, from three months of age until puberty, and from 90 days of gestation until farrowing. Early studies showed that a 20% feed restriction from 90 days of age until puberty drastically reduces mammary parenchymal tissue mass. Yet, in a more recent study, sow milk yield was not altered following a 10% or 20% feed restriction, or a 25% dietary fibre addition from 90 days of age to breeding. This absence of effect was likely due to the greater feed intake of control gilts in that recent study compared with the older studies, and suggested that feed intake of growing gilts can be reduced to 2.7 kg/d (but not 2.1 kg/d) without detrimental effects on future milk yield. During prepuberty, inclusion of the phytoestrogen genistein in the diet increases the number of mammary parenchymal cells. During late gestation, feeding very high energy levels may have detrimental effects on mammary development and subsequent milk production. Feed intake throughout gestation is also important because of its effect on body condition, with gilts that are too thin (< 16 mm backfat thickness) in late gestation showing less mammary development. A 40% increase in lysine intake via inclusion of additional soybean meal to the diet of gilts from days 90 to 110 of gestation increased mammary parenchymal mass by 44%. Increasing circulating concentrations of the growth factor IGF-1 during late gestation also increased mammary parenchymal mass by 22%. Current data clearly demonstrate that feeding management before lactation can be used to enhance mammary development, hence future milk yield.(AU)
Assuntos
Animais , Feminino , Gravidez , Suínos/fisiologia , Glândulas Mamárias Animais/crescimento & desenvolvimento , Otimização de ProcessosResumo
The objective of this study was to determine pH and the concentrations of Calcium (Ca2+), Magnesium (Mg2+), Chloride (Cl-), Sodium (Na+), Potassium (K+) and Calcium (Ca2+) in the mammary gland secretion in pre-foaling Mangalarga Marchador (MM) mares to determine the best method to predict parturition in the breed. Forty-two pregnant MM mares were evaluated once daily from 310 days of gestation until parturition. Mammary gland secretion pH, measured by pH meter and pH paper strip, Mg2+, Ca2+ and Cl- levels significantly changed during the last 10 days prior to parturition (p<0.05). The lowest pH mean value (pH meter=6.67±0.42; pH strip=6.23±0.53) and significantly higher mean concentrations of Ca2+ (12.6±5.8mmol/L) and Mg2+ (10.93±3.66mmol/L) were obtained 24 hours prior to foaling (p<0.0001). Chloride levels reduced significantly 8 days prior to parturition (p<0.0001) but remained unchanged until the due date. Mammary gland pH secretion, measured by pH meter and pH paper strip, was effective to predict parturition. Quantitative pH values obtained with the pH meter had a greater accuracy in comparison to pH paper strips, but a strong correlation was found between both methods. Calcium and magnesium were the only electrolytes that changed in concentration immediately prior to parturition and are better indicators of when not to expect foaling.
O objetivo deste estudo foi determinar o pH e as concentrações de cálcio (Ca2+), magnésio (Mg2+), cloro (Cl-), sódio (Na+) e potássio (K+) na secreção da glândula mamária de éguas Mangalarga Marchador (MM) no período pré-parto, a fim de determinar o melhor método para predizer proximidade do parto na raça. Quarenta e duas éguas MM foram avaliadas diariamente a partir dos 310 dias de gestação até o parto. O pH, aferido pelo pHmetro e por fitas reagentes, e os eletrólitos Ca2+, Mg2+ e Cl- apresentaram diferenças significativas em suas concentrações nos últimos 10 dias pré-parto (P<0,05). A diminuição significativa dos valores médios de pH (pHmetro= 6,67±0,42; fitas reagentes= 6,23±0,53) e o aumento significativo nas concentrações médias de Ca2+ (12,6±5,8mmol/L) e Mg2+ (10,93±3,66mmol/L) foram observados 24 horas pré-parto (P<0,0001). As concentrações de cloro diminuíram significativamente oito dias antes do parto (P<0,0001), porém continuaram sem alteração até o momento da parição. O pH da secreção foi eficaz para predizer o momento do parto. Valores quantitativos obtidos por meio do pHmetro apresentaram maior acurácia em comparação às fitas de pH, porém obteve-se forte correlação entre os dois métodos. Cálcio e magnésio foram os únicos eletrólitos que apresentaram alterações significativas no pré-parto imediato e são indicadores mais precisos de quando não esperar o parto.
Assuntos
Animais , Secreções Corporais , Parto , Eletrólitos , CavalosResumo
Estradiol cypionate (EC) or GnRH have been widely used for ovulation induction in timed embryo transfer (TET). EC administration increases the proportion of cows that show estrus, whereas GnRH promotes more synchronized ovulations. The aim of the present study was to evaluate the potential beneficial effects of combining EC and GnRH in TET. In experiment 1, no difference was observed on serum progesterone concentrations on Day 6 and 13 after GnRH treatment between GnRH and EC+GnRH groups. In experiment 2, pregnancy per embryo transfer (P/ET) did not differ (p = 0.69) between GnRH (62.8%) and EC+GnRH (58.7%) groups. In conclusion, combining EC and GnRH for ovulation induction does not increase progesterone secretion and pregnancy rate after TET in cattle.(AU)
Assuntos
Animais , Feminino , Gravidez , Indução da Ovulação/veterinária , Hormônio Liberador de Gonadotropina/análogos & derivados , Estradiol/análogos & derivados , Transferência Embrionária/instrumentaçãoResumo
Estrogens and progestogens are hormones produced by maternal organs and it is required for recognition and maintenance of pregnancy. In addition, the embryo may also be a source. For this, the aim was to identify steroidogenic expression on Galea spixii embryos early in the embryonic period. Embryos were collected on Days 10 and 15 of gestation; some were fixed in 4% paraformaldehyde for morphological and immunohistochemical analysis (P450arom), whereas others had RNA extracted to determine presence of CYP19a1 gene. In addition, for immunochemistry, maternal ovaries were collected as positive control tissues. Maternal tissues had positive staining for aromatase, whereas none of the embryos stained for P450 aromatase. Based on qPCR reactions, CYP19a1 gene were expressed in all embryos. Galea spixii embryos expressed steroidogenic genes during the post-implantation period, indicating they have the potential to produce steroid hormones.(AU)
Assuntos
Animais , Feminino , Roedores/embriologia , Hormônios Esteroides Gonadais/efeitos adversos , Aromatase/químicaResumo
Background: During pregnancy, cardiovascular adaptations occur in order to meet maternal demands and adequate supportfor fetal development, but they are still unclear in the equine species, especially in the American Miniature horse breed.The main hemodynamic changes that occur during pregnancy are increased heart rate, cardiac output, and blood volume.It is necessary to use the reference values for the miniature breeds, in addition to the age range and reproductive condition,so the objective of this study was to evaluate the effect of pregnancy on electrocardiographic (ECG) parameters in maresand also of age in newborns foals of American Miniature horse breed.Materials, Methods & Results: Ten American Miniature Horse mares, between 7 and 19 years old (12.14 ± 4.05 years),116.85 ± 24.09 kg of body weight were used in the experiment. All these mares were pluriparous, with 5.9 ± 3.5 of births,minimum of 2, and maximum of 12 foaling times. They were maintained in common social group in an indoor house stablewith straw bed, with access to an outdoor paddock for several h per day. Twice a day they were fed with hay. Mineral supplements and water were available ad libitum. The ECG were obtained in 10 pregnant mares and in 10 neonates. All femaleswere examined at a representative time of each trimester of pregnancy, at 30, 210, 300 days before and 21 days after delivery.Neonates were examined on different times: first 24 h after delivery, weekly on the 7th, 14th, 21st, 28th and 35th days of age.The ECG parameters considered were the heart rate and rhythm, duration and intervals (P, PR, QRS, QT, T) and amplitude(P, R, S, T) of ECG waves. The mean of pregnancy duration was 319.4 ± 4.97 days. Heart Rate (HR) varied between 51 to59 bpm during pregnancy and 56 bpm on the 21st day after delivery. Comparing the clinical parameters of pregnant mares,non-pregnant mares and foal a difference was found for HR, Respiration Rate (RR) and...
Assuntos
Feminino , Animais , Cavalos , Eletrocardiografia/veterinária , Prenhez , Eletrodiagnóstico/veterináriaResumo
Background: During pregnancy, cardiovascular adaptations occur in order to meet maternal demands and adequate supportfor fetal development, but they are still unclear in the equine species, especially in the American Miniature horse breed.The main hemodynamic changes that occur during pregnancy are increased heart rate, cardiac output, and blood volume.It is necessary to use the reference values for the miniature breeds, in addition to the age range and reproductive condition,so the objective of this study was to evaluate the effect of pregnancy on electrocardiographic (ECG) parameters in maresand also of age in newborns foals of American Miniature horse breed.Materials, Methods & Results: Ten American Miniature Horse mares, between 7 and 19 years old (12.14 ± 4.05 years),116.85 ± 24.09 kg of body weight were used in the experiment. All these mares were pluriparous, with 5.9 ± 3.5 of births,minimum of 2, and maximum of 12 foaling times. They were maintained in common social group in an indoor house stablewith straw bed, with access to an outdoor paddock for several h per day. Twice a day they were fed with hay. Mineral supplements and water were available ad libitum. The ECG were obtained in 10 pregnant mares and in 10 neonates. All femaleswere examined at a representative time of each trimester of pregnancy, at 30, 210, 300 days before and 21 days after delivery.Neonates were examined on different times: first 24 h after delivery, weekly on the 7th, 14th, 21st, 28th and 35th days of age.The ECG parameters considered were the heart rate and rhythm, duration and intervals (P, PR, QRS, QT, T) and amplitude(P, R, S, T) of ECG waves. The mean of pregnancy duration was 319.4 ± 4.97 days. Heart Rate (HR) varied between 51 to59 bpm during pregnancy and 56 bpm on the 21st day after delivery. Comparing the clinical parameters of pregnant mares,non-pregnant mares and foal a difference was found for HR, Respiration Rate (RR) and...(AU)
Assuntos
Animais , Feminino , Cavalos , Prenhez , Eletrocardiografia/veterinária , Eletrodiagnóstico/veterináriaResumo
O transcorrer do parto é de grande importância para a preservação da vida materna e dos filhotes em cadelas, destacando-se a distocia como principal causa de mortalidade neonatal. De forma a identificar precocemente indícios de distocia e reduzir complicações, recomenda-se o acompanhamento gestacional e a previsão da data do parto mediante técnicas ultrassonográficas. A avaliação e mensuração das estruturas fetais podem ser realizadas a fim de determinar o estágio e idade gestacional, deduzindo a provável data de parto. A frequência cardíaca fetal (FCF) é fundamental para o acompanhamento da viabilidade fetal e também auxilia na predição do momento do parto quando oscilações na FCF estão presentes. O modo Doppler é relevante na avaliação gestacional, uma vez que permite conhecer as características do fluxo arterial umbilical e avaliar suas alterações durante a gestação, aumentando a precisão de estimativa da data de parto mediante a avaliação do índice de resistividade umbilical. Por isso, o presente trabalho busca revisar e explorar as metodologias ultrassonográficas de análise gestacional, demonstrando que o exame ultrassonográfico pode ser utilizado como ferramenta na determinação precoce e precisa do momento do parto e, dessa forma, possibilita um planejamento mais acurado com o objetivo de aumentar o índice de sucesso nos partos, bem como intervir caso haja necessidade.(AU)
The course of delivery holds great importance on the preservation of maternal and offspring life in female dogs, being dystocia the main cause of neonatal mortality. In order to identify early indicators of dystocia and reduce complications in the partum, gestational follow-up and the prediction of a more precise delivery date are recommended. The evaluation and measurement of fetal structures can be performed to determine the stage and gestational age, and estimate the delivery date. Fetal heart rate (FHR) is fundamental for monitoring fetal viability in dogs, assisting in prediction of the delivery time when oscillations in the FHR are presents. Doppler mode is a relevant tool for the gestational assessment, once it measures the umbilical arterial flow and its alterations during the gestational period, also increasing the accuracy of the birth date by estimating the umbilical resistivity index. Therefore, this article seeks to review and explore ultrasound methods for gestational evaluation, demonstrating that ultrasound examination can be used as a tool for early and precise determination of delivery time and, consequently, allowing planning and prepare to a higher rate of success in deliveries, as well as intervention and assistance when necessary.(AU)
Assuntos
Animais , Feminino , Gravidez , Cães , Parto , Cães , Ultrassonografia/métodos , Ultrassonografia/veterinária , Frequência Cardíaca FetalResumo
O rebanho Girolando tem crescido substancialmente nas últimas décadas no Brasil, tornando a raça uma das mais selecionadas e visadas para a exploração de leite entre os criadores. No entanto, é sabido a importância do manejo das fêmeas para evitar perdas durante a gestação, sendo elevado os abortos e as distocias no periparto, por desconhecimento da previsão de partos. Mas para estabelecer uma tabela de previsão de partos é necessário conhecer o período de gestação de raça explorada. Neste contexto, este estudo envolveu observações de três décadas (1990 a 2019) de avaliação dos efeitos de alguns fatores (ano de parto, sexo da cria, grupo sanguíneo materno, raça paterna e ordem de parto) sobre os períodos de gestação (PG) do rebanho Girolando do Centro Estadual de Pesquisa em Agricultura Orgânica (CEPAO), da Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (Pesagro-Rio). Para isso, foram utilizados os dados dos registros do rebanho dos sistemas de fichários e software Gera leite® , relativos aos PG (dia) de vacas Girolando, segundo as variáveis (1) ano, (2) sexo, (3) grupo sanguíneo materno, (4) raça paterno e (5) ordem de parto, tabulados e submetidos a análises descritivas, a anova one-way e teste Bonferroni no software Bioestat. Os métodos reprodutivos utilizados foram as inseminações, realizadas com a observação do estro, seguindo o sistema tradicional de Trimberg e, na última década, também a inseminação artificial em tempo fixo (IATF), com a utilização de protocolos hormonais para sincronização da ovulação. Os resultados médios, da avaliação de 2.104 partos não mostraram diferenças significativas (p>0,05), nos efeitos dos fatores analisados sobre os períodos de gestação. Assim, a média geral de 283,2±6,5 dias, pode ser considerada como uma estimativa dos partos esperados para o rebanho Girolando da Pesagro, a fim de proporcionar manejo e assistência adequados durante o parto, evitando distocias e perdas de mortalidade no periparto.(AU)
The Girolando herd has grown substantially in recent decades in Brazil, making the breed one of the most selected and targeted for milk exploration among breeders. However, it is known the importance of handling females to avoid losses during pregnancy, with abortions and dystocia in the peri-parturition, being high due to the lack of birth prediction. However, to establish a birth prediction table, it is necessary to know the gestation period of the exploited race. In this context, this study involved observations from three decades (1990 to 2019) to assess the effects of some factors (year of birth, sex of offspring, maternal blood group, paternal race and birth order), on gestation periods (PG) of the Girolando herd of the Centro Estadual de Pesquisa Agricultura Orgânica (CEPAO) of Pesagro-Rio. It is located in the municipality of Seropédica, RJ, in the Baixada Fluminense region, where in general the climate presents high temperatures (maximum ± 30oC) and relative air humidity (maximum < 60%). For this, data from the herd records of the binder systems and Gera leite® software were used, related to the PG (day) of Girolando cows, according to (1) year, (2) sex, (3) maternal blood group, (4) paternal race and (5) birth order were tabulated and submitted to descriptive analyzes, the one-way anova and Bonferroni test in the Bioestat software. The reproductive methods used were inseminations, performed with the observation of estrus, following the traditional Trimberg system and, in the last decade, also artificial insemination in fixed time (TAI), with the use of hormonal protocols for synchronization of ovulation). The results of the evaluation did not show significant differences (p>0.05), in the effects of the analyzed factors, on the gestation periods. Thus, the general average of 283.2±6.5 days can be considered as an estimate of expected deliveries for the Pesagro Girolando herd, in order to provide adequate management and assistance during childbirth, avoid dystocias and mortality losses in the peripartum.(AU)
Assuntos
Animais , Feminino , Gravidez , Bovinos , Bovinos/fisiologia , Saúde Reprodutiva , Prenhez/fisiologiaResumo
O rebanho Girolando tem crescido substancialmente nas últimas décadas no Brasil, tornando a raça uma das mais selecionadas e visadas para a exploração de leite entre os criadores. No entanto, é sabido a importância do manejo das fêmeas para evitar perdas durante a gestação, sendo elevado os abortos e as distocias no periparto, por desconhecimento da previsão de partos. Mas para estabelecer uma tabela de previsão de partos é necessário conhecer o período de gestação de raça explorada. Neste contexto, este estudo envolveu observações de três décadas (1990 a 2019) de avaliação dos efeitos de alguns fatores (ano de parto, sexo da cria, grupo sanguíneo materno, raça paterna e ordem de parto) sobre os períodos de gestação (PG) do rebanho Girolando do Centro Estadual de Pesquisa em Agricultura Orgânica (CEPAO), da Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (Pesagro-Rio). Para isso, foram utilizados os dados dos registros do rebanho dos sistemas de fichários e software Gera leite® , relativos aos PG (dia) de vacas Girolando, segundo as variáveis (1) ano, (2) sexo, (3) grupo sanguíneo materno, (4) raça paterno e (5) ordem de parto, tabulados e submetidos a análises descritivas, a anova one-way e teste Bonferroni no software Bioestat. Os métodos reprodutivos utilizados foram as inseminações, realizadas com a observação do estro, seguindo o sistema tradicional de Trimberg e, na última década, também a inseminação artificial em tempo fixo (IATF), com a utilização de protocolos hormonais para sincronização da ovulação. Os resultados médios, da avaliação de 2.104 partos não mostraram diferenças significativas (p>0,05), nos efeitos dos fatores analisados sobre os períodos de gestação. Assim, a média geral de 283,2±6,5 dias, pode ser considerada como uma estimativa dos partos esperados para o rebanho Girolando da Pesagro, a fim de proporcionar manejo e assistência adequados durante o parto, evitando distocias e perdas de mortalidade no periparto.
The Girolando herd has grown substantially in recent decades in Brazil, making the breed one of the most selected and targeted for milk exploration among breeders. However, it is known the importance of handling females to avoid losses during pregnancy, with abortions and dystocia in the peri-parturition, being high due to the lack of birth prediction. However, to establish a birth prediction table, it is necessary to know the gestation period of the exploited race. In this context, this study involved observations from three decades (1990 to 2019) to assess the effects of some factors (year of birth, sex of offspring, maternal blood group, paternal race and birth order), on gestation periods (PG) of the Girolando herd of the Centro Estadual de Pesquisa Agricultura Orgânica (CEPAO) of Pesagro-Rio. It is located in the municipality of Seropédica, RJ, in the Baixada Fluminense region, where in general the climate presents high temperatures (maximum ± 30oC) and relative air humidity (maximum 0.05), in the effects of the analyzed factors, on the gestation periods. Thus, the general average of 283.2±6.5 days can be considered as an estimate of expected deliveries for the Pesagro Girolando herd, in order to provide adequate management and assistance during childbirth, avoid dystocias and mortality losses in the peripartum.
Assuntos
Feminino , Animais , Gravidez , Bovinos , Bovinos/fisiologia , Prenhez/fisiologia , Saúde ReprodutivaResumo
O transcorrer do parto é de grande importância para a preservação da vida materna e dos filhotes em cadelas, destacando-se a distocia como principal causa de mortalidade neonatal. De forma a identificar precocemente indícios de distocia e reduzir complicações, recomenda-se o acompanhamento gestacional e a previsão da data do parto mediante técnicas ultrassonográficas. A avaliação e mensuração das estruturas fetais podem ser realizadas a fim de determinar o estágio e idade gestacional, deduzindo a provável data de parto. A frequência cardíaca fetal (FCF) é fundamental para o acompanhamento da viabilidade fetal e também auxilia na predição do momento do parto quando oscilações na FCF estão presentes. O modo Doppler é relevante na avaliação gestacional, uma vez que permite conhecer as características do fluxo arterial umbilical e avaliar suas alterações durante a gestação, aumentando a precisão de estimativa da data de parto mediante a avaliação do índice de resistividade umbilical. Por isso, o presente trabalho busca revisar e explorar as metodologias ultrassonográficas de análise gestacional, demonstrando que o exame ultrassonográfico pode ser utilizado como ferramenta na determinação precoce e precisa do momento do parto e, dessa forma, possibilita um planejamento mais acurado com o objetivo de aumentar o índice de sucesso nos partos, bem como intervir caso haja necessidade.
The course of delivery holds great importance on the preservation of maternal and offspring life in female dogs, being dystocia the main cause of neonatal mortality. In order to identify early indicators of dystocia and reduce complications in the partum, gestational follow-up and the prediction of a more precise delivery date are recommended. The evaluation and measurement of fetal structures can be performed to determine the stage and gestational age, and estimate the delivery date. Fetal heart rate (FHR) is fundamental for monitoring fetal viability in dogs, assisting in prediction of the delivery time when oscillations in the FHR are presents. Doppler mode is a relevant tool for the gestational assessment, once it measures the umbilical arterial flow and its alterations during the gestational period, also increasing the accuracy of the birth date by estimating the umbilical resistivity index. Therefore, this article seeks to review and explore ultrasound methods for gestational evaluation, demonstrating that ultrasound examination can be used as a tool for early and precise determination of delivery time and, consequently, allowing planning and prepare to a higher rate of success in deliveries, as well as intervention and assistance when necessary.
Assuntos
Feminino , Animais , Gravidez , Cães , Cães , Frequência Cardíaca Fetal , Parto , Ultrassonografia/métodos , Ultrassonografia/veterináriaResumo
Uterine torsion can be defined as the rotation of the uterine horn or body of the uterus, and it can be present in both clockwise and counterclockwise, its rotation can vary which directly affects the prognosis. It is a rare condition in bitches, and pregnancy is a factor commonly involved in this condition. The ovariosalpingohisterectomy (OSH) technique is the treatment of choice. A bitch of two years old of Pinscher breed was attend with a history of copulafor approximately 30 days ago, manifesting anorexia, apathy and abdominal pain for two days. Animal was hypothermic, dehydrated, hypotensive, hypoglycemic and presented neutrophilia lynfocytosis with a left shift. By performing the ultrasound examination, the presence of dead fetuses was observed. It was decided to initially stabilize the patient, subsequent OSH surgery was performed. Antibiotics and analgesics were administered during the hospitalization period, observing an improvement in the condition on the third day. The patient was discharged from hospital and a good recovery was observed on return. Despite the rare involvement of bitches in cases of uterine torsion, pregnancy can increase the risk of occurrences, the rapid intervention is of paramount importancef or the prognosis, since in this case the treatment proposed by the literature was carried out, obtaining the total recovery of the patient.
Assuntos
Feminino , Animais , Gravidez , Cães , Doenças do Cão/cirurgia , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/sangue , Histerectomia/veterinária , Útero/anormalidadesResumo
Uterine torsion can be defined as the rotation of the uterine horn or body of the uterus, and it can be present in both clockwise and counterclockwise, its rotation can vary which directly affects the prognosis. It is a rare condition in bitches, and pregnancy is a factor commonly involved in this condition. The ovariosalpingohisterectomy (OSH) technique is the treatment of choice. A bitch of two years old of Pinscher breed was attend with a history of copulafor approximately 30 days ago, manifesting anorexia, apathy and abdominal pain for two days. Animal was hypothermic, dehydrated, hypotensive, hypoglycemic and presented neutrophilia lynfocytosis with a left shift. By performing the ultrasound examination, the presence of dead fetuses was observed. It was decided to initially stabilize the patient, subsequent OSH surgery was performed. Antibiotics and analgesics were administered during the hospitalization period, observing an improvement in the condition on the third day. The patient was discharged from hospital and a good recovery was observed on return. Despite the rare involvement of bitches in cases of uterine torsion, pregnancy can increase the risk of occurrences, the rapid intervention is of paramount importancef or the prognosis, since in this case the treatment proposed by the literature was carried out, obtaining the total recovery of the patient.(AU)
Assuntos
Animais , Feminino , Gravidez , Cães , Doenças do Cão/sangue , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/cirurgia , Útero/anormalidades , Histerectomia/veterináriaResumo
Pluripotent stem cells have been studied as source of cells for regenerative medicine and acquire or genetic diseases, as an innovative therapy. Most tissues have stem cells populations, however in few quantities or impossible to be used during adult life, which lead to scientists look for new sources. Thus, this study aimed to analyze the presence of pluripotent cells in the uterus and placenta, following up non-pregnant, pregnant (begin, middle, and final), and postpartum periods in dogs. The uteri were obtained from social castration programs for population control in Pirassununga, Sao Paulo, Brazil. It was collected 20 uteri at different stages. The samples were fixed and processed for immunohistochemical analysis of NANOG, OCT4 and SOX2 expression, knowing as pluripotent stem cells makers. Our results showed positive expression for NANOG, OCT4 and SOX2 in all stages of gestation and nonpregnant uterus; however, we highlight some quantitative different between stages. OCT4 showed more expression in non-pregnant uterus than NANOG and SOX2, and its expression increased in pregnant uterus. In pregnant uterus there was more expression of NANOG than OCT4 and SOX2. Interesting, no difference was found between these markers in the other periods. In conclusion, it was possible to identify pluripotent stem cells in all periods in dog placenta and uterus, however during the early stage of pregnancy we observed more pluripotent stem cells than in all the others periods confirming the high plasticity and regeneration capacity of the uterine tissue.
Assuntos
Feminino , Animais , Cães , Cães/fisiologia , Células-Tronco Pluripotentes , PlacentaçãoResumo
Pluripotent stem cells have been studied as source of cells for regenerative medicine and acquire or genetic diseases, as an innovative therapy. Most tissues have stem cells populations, however in few quantities or impossible to be used during adult life, which lead to scientists look for new sources. Thus, this study aimed to analyze the presence of pluripotent cells in the uterus and placenta, following up non-pregnant, pregnant (begin, middle, and final), and postpartum periods in dogs. The uteri were obtained from social castration programs for population control in Pirassununga, Sao Paulo, Brazil. It was collected 20 uteri at different stages. The samples were fixed and processed for immunohistochemical analysis of NANOG, OCT4 and SOX2 expression, knowing as pluripotent stem cells makers. Our results showed positive expression for NANOG, OCT4 and SOX2 in all stages of gestation and nonpregnant uterus; however, we highlight some quantitative different between stages. OCT4 showed more expression in non-pregnant uterus than NANOG and SOX2, and its expression increased in pregnant uterus. In pregnant uterus there was more expression of NANOG than OCT4 and SOX2. Interesting, no difference was found between these markers in the other periods. In conclusion, it was possible to identify pluripotent stem cells in all periods in dog placenta and uterus, however during the early stage of pregnancy we observed more pluripotent stem cells than in all the others periods confirming the high plasticity and regeneration capacity of the uterine tissue.(AU)
Assuntos
Animais , Feminino , Cães , Cães/fisiologia , Células-Tronco Pluripotentes , PlacentaçãoResumo
An inguinal hernia may result in protrusion of the uterus through the hernial ring, thus being called a hysterocele. If the female is pregnant, the fetuses viability may be compromised, through the incarceration of the uterus in the hernial ring, in addition to harming the females systemic homeostasis. Complementary imaging tests, such as ultrasound, are of fundamental importance for the diagnosis and prognosis of the case, with surgery being the recommended treatment. The aim of the present work is to report the case of an eight-year-old female pinscher, diagnosed with a gravid hysterocele, treated by inguinal herniorrhaphy. The patient reached the end of pregnancy with viable fetuses.
A hérnia inguinal pode ter como consequência a protusão do útero pelo canal herniário, denominando-se, assim, histerocele. Caso a fêmea esteja gestante, a viabilidade dos fetos pode ser comprometida, por meio do encarceramento do útero no canal herniário, além de prejudicar a homeostasia sistêmica da fêmea. Exames complementares de imagem, como ultrassom, são de fundamental importância para diagnóstico e prognóstico do caso, sendo a cirurgia o tratamento preconizado. Objetiva-se com o presente trabalho relatar o caso de uma cadela, pinscher, de oito anos, diagnosticada com histerocele gravídica, tratada mediante herniorrafia inguinal. A paciente chegou ao término da gestação com os fetos viáveis.
Assuntos
Feminino , Animais , Cães , Cães/sangue , Hérnia Inguinal , PrenhezResumo
An inguinal hernia may result in protrusion of the uterus through the hernial ring, thus being called a hysterocele. If the female is pregnant, the fetuses viability may be compromised, through the incarceration of the uterus in the hernial ring, in addition to harming the females systemic homeostasis. Complementary imaging tests, such as ultrasound, are of fundamental importance for the diagnosis and prognosis of the case, with surgery being the recommended treatment. The aim of the present work is to report the case of an eight-year-old female pinscher, diagnosed with a gravid hysterocele, treated by inguinal herniorrhaphy. The patient reached the end of pregnancy with viable fetuses.(AU)
A hérnia inguinal pode ter como consequência a protusão do útero pelo canal herniário, denominando-se, assim, histerocele. Caso a fêmea esteja gestante, a viabilidade dos fetos pode ser comprometida, por meio do encarceramento do útero no canal herniário, além de prejudicar a homeostasia sistêmica da fêmea. Exames complementares de imagem, como ultrassom, são de fundamental importância para diagnóstico e prognóstico do caso, sendo a cirurgia o tratamento preconizado. Objetiva-se com o presente trabalho relatar o caso de uma cadela, pinscher, de oito anos, diagnosticada com histerocele gravídica, tratada mediante herniorrafia inguinal. A paciente chegou ao término da gestação com os fetos viáveis.(AU)
Assuntos
Animais , Feminino , Cães , Cães/sangue , Hérnia Inguinal , PrenhezResumo
O objetivo do presente estudo foi identificar os parâmetros bioquímicos do líquido amniótico (LA) no momento do parto de éguas com placentite ascendente e relacionar com parâmetros bioquímicos séricos dos neonatos. Foram utilizadas 36 éguas gestantes da raça Puro Sangue Inglês, divididas em dois grupos: placentite (n=5) e sadias (n=31). A placentite foi definida de acordo com a avaliação clínica, exame ultrassonográfico e histopatologia das placentas. Essas éguas foram submetidas a terapia antimicrobiana, antiinflamatórios e altrenogest. A coleta do LA foi realizada na segunda fase do parto e as amostras sanguíneas dos neonatos imediatamente após o nascimento. Foi observada menor concentração de creatinina no LA de éguas com placentite. Potros provenientes de éguas com placentite foram menores em peso e altura ao nascimento, e apresentaram concentrações elevadas de ureia e reduzidas de PPT na avaliação sérica. Foi observado correlação positiva moderada entre creatinina e ureia no LA e correlação positiva fraca entre a creatinina do LA e sérica dos potros. Concluiu-se que o perfil bioquímico do LA e sérico dos neonatos oriundos de éguas com placentite ascendente diferem de gestações sadias. Sendo que esses parâmetros podem ser utilizados como indicadores de alterações placentárias em éguas.(AU)
The aim of this study was to identify the biochemical parameters of amniotic fluid (AF) at parturition in mares with ascending placentitis, and to relate with biochemical parameters in neonatal foals serum. Thirty-six pregnant Thoroughbred mares were divided in 2 groups: mares with placentitis (n=5) and healthy mares (n=31). Placentitis was identified based on clinical signs, ultrasound evaluation and histopathological features in the placenta. Mares with placentitis were treated with antibiotics, antiinflammatories and altrenogest. AF was collected during the second stage of labor and blood samples were taken immediately after foaling. Lower concentrations of creatinine were found in the AF of mares with placentitis. Foals from mares with placentitis showed smaller height and lower birthweight, also showed increase of urea and decrease of total protein levels in blood serum. A moderate positive correlation between urea and creatinine in AF was observed, and a weak positive correlation was found between creatinine in AF and the serum of foals. In conclusion, biochemical composition of AF and of blood serum of neonatal foals from mares with ascending placentitis are different of healthy mares. So, those analysis could be useful to identify placental changes in mares.(AU)
Assuntos
Animais , Feminino , Cavalos/fisiologia , Biomarcadores , Líquido Amniótico , Soro , Creatinina , Ureia , PrenhezResumo
O objetivo do presente estudo foi identificar os parâmetros bioquímicos do líquido amniótico (LA) no momento do parto de éguas com placentite ascendente e relacionar com parâmetros bioquímicos séricos dos neonatos. Foram utilizadas 36 éguas gestantes da raça Puro Sangue Inglês, divididas em dois grupos: placentite (n=5) e sadias (n=31). A placentite foi definida de acordo com a avaliação clínica, exame ultrassonográfico e histopatologia das placentas. Essas éguas foram submetidas a terapia antimicrobiana, antiinflamatórios e altrenogest. A coleta do LA foi realizada na segunda fase do parto e as amostras sanguíneas dos neonatos imediatamente após o nascimento. Foi observada menor concentração de creatinina no LA de éguas com placentite. Potros provenientes de éguas com placentite foram menores em peso e altura ao nascimento, e apresentaram concentrações elevadas de ureia e reduzidas de PPT na avaliação sérica. Foi observado correlação positiva moderada entre creatinina e ureia no LA e correlação positiva fraca entre a creatinina do LA e sérica dos potros. Concluiu-se que o perfil bioquímico do LA e sérico dos neonatos oriundos de éguas com placentite ascendente diferem de gestações sadias. Sendo que esses parâmetros podem ser utilizados como indicadores de alterações placentárias em éguas.
The aim of this study was to identify the biochemical parameters of amniotic fluid (AF) at parturition in mares with ascending placentitis, and to relate with biochemical parameters in neonatal foals serum. Thirty-six pregnant Thoroughbred mares were divided in 2 groups: mares with placentitis (n=5) and healthy mares (n=31). Placentitis was identified based on clinical signs, ultrasound evaluation and histopathological features in the placenta. Mares with placentitis were treated with antibiotics, antiinflammatories and altrenogest. AF was collected during the second stage of labor and blood samples were taken immediately after foaling. Lower concentrations of creatinine were found in the AF of mares with placentitis. Foals from mares with placentitis showed smaller height and lower birthweight, also showed increase of urea and decrease of total protein levels in blood serum. A moderate positive correlation between urea and creatinine in AF was observed, and a weak positive correlation was found between creatinine in AF and the serum of foals. In conclusion, biochemical composition of AF and of blood serum of neonatal foals from mares with ascending placentitis are different of healthy mares. So, those analysis could be useful to identify placental changes in mares.
Assuntos
Feminino , Animais , Biomarcadores , Cavalos/fisiologia , Creatinina , Líquido Amniótico , Prenhez , Soro , UreiaResumo
O presente trabalho objetivou o acompanhamento da gestação inicial na égua entre os dias 12 a35 pós-ovulação por meio da ultrassonografia tridimensional (3D). Após o diagnóstico de gestação no dia12 pós-ovulação, a reprodutora foi examinada diariamente por palpação retal e ultrassonografia paraadquirir imagens no modo B e em 3D. Nas avaliações diárias e com as imagens construídas pela técnicade ultrassonografia tridimensional foi possível acompanhar o crescimento do concepto, a identificação doembrião propriamente dito e a diferenciação de estruturas e membranas (saco vitelino e saco alantoide)permitindo visualizações em 360 graus. Pode-se concluir que na técnica de processamento da imagemtridimensional as imagens geradas fornecem excelente material educacional e acadêmico, precisando demais estudos para permitir uma efetiva aplicação clínica na perda embrionária.(AU)
The present study aimed at monitoring the initial pregnancy in the mare between days 12 to 35after ovulation using three-dimensional (3D) ultrasonography. After the diagnosis of gestation on day 12after ovulation, the reproductive tract was examined daily by rectal palpation and ultrasonography toacquire images in B mode and in 3D. In the daily evaluations and with the images constructed by thetechnique of three-dimensional ultrasonography, it was possible to follow the growth of the concept, theidentification of the embryo proper and the differentiation of structures and membranes (yolk andallantoic sac) allowing visualizations in 360 degrees. It can be concluded that the 3D generated imagesprovide excellent educational and academic material however, more studies are needed to allow aneffective application in embryonic loss.(AU)
Assuntos
Animais , Feminino , Gravidez , Cavalos/embriologia , Prenhez/fisiologia , Ultrassonografia Pré-Natal/métodos , Ultrassonografia Pré-Natal/veterinária , Desenvolvimento EmbrionárioResumo
Background: Musculoskeletal changes in growing foals can be linked to metabolic disorders which affect the cartilage metabolism associated of obesity during the late gestation of the mares, negatively affecting the athletic performance of the otherwise prospective foals. High basal insulin levels can be associated with increased weight and obesity of the mares, altering the supply of the glucose to the fetus and the production of IGF-1, which plays an important role in the endochondral cartilage metabolism. The present study aims to describe the association of metabolic and biometric alterations in overweight Criollo mares with IGF-1 levels and the presence of articular and physeal lesions in their foals. Materials, Methods & Results: A prospective and observational cohort study was conducted using the foals and the overweight mares at field conditions. Twenty-eight foals and their overweight mothers were utilized. The foals biometric and metabolic features as weight and height; glucose, total cholesterol, cholesterol HDL, triglycerides, basal insulin, leptin, glucose, total T4 and IGF-1 were analyzed, from the birth until the weaning period around sixth month of life of it. All the foals, at the weaning period, were submitted to the radiograph examination of the tarsal joints and the metacarpal-phalangeal joints to evaluate signs of lesions and the possible distal metacarpal physeal changes, respectively. The mares biometric and metabolic features as weight, height and fat tail-head deposition; basal insulin, leptin, glucose, total cholesterol, cholesterol HDL and triglycerides were analyzed at the final period of gestation. The basal insulin levels of the foals at the second, fourth and sixth month of life were 9.87 ± 1.82 µUI/mL, 9.13 ± 1.94 µUI/mL, 9.39 ± 2.54 µUI/mL. The IGF-1 levels of the foals at the second, third, fourth, fifth and sixth...