Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats

PURPOSE:To evaluate the role of low molecular chitosan containing sepia ink (LMCS) in ethanol-induced (5 ml/kg) gastric ulcer in rats.METHODS:Animals were divided into four groups (n = 12): normal group (Normal), negative control group (Con), experiment group (LMCS) and positive control Omeprazole group (OMZ). Gastric empty rate was detected in the first 7 days. Rats were sacrificed at 7, 14 and 21 day for histology and ELISA detections.RESULTS:Gastric empty was no significant differences among the groups (P > 0.05). Histological observation showed gastric mucosal LMCS treated had better healing effect. Hydroxyproline (Hyp) was significantly increased from 7 day (P < 0.05). LMCS significantly inhibited malondialdehyde (MDA) generation for lipid peroxidation from 7 day (P < 0.05). LMCS significantly promoted the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) at the earlier stage (P < 0.05). OMZ had the similar effects above. As for myeloperoxidase (MPO), LMCS significantly decreased and restored it to normal levels from 7 day (P < 0.05), it is earlier than OMZ which is from 14 day.CONCLUSION:LMCS can improve gastric mucosa tissue repair, exert significant influences on oxidative and antioxidant enzyme activities and neutrophil infiltration.(AU)

Experimental model of Achilles tendon injury in rats

PURPOSE: To describe an effective experimental model to study the Achilles tendon healing. METHODS: Forty male Rattus norvegicus albinus, Wistar lineage adult male weighing 250 to 300g were used for this experiment and thirty were surgically submitted to bilateral partial transverse section of the Achilles tendon. The right tendon was treated with radio waves (RF) whereas the left tendon served as control. On the third postoperative day, the rats were divided into four experimental groups consisting of ten rats each which were treated with monopolar RF adjusted to 650 kHz and 2w, for two minutes twice a week and a group of normal animals without any intervention, until they were sacrificed on the 7th, 14th and 28th days, respectively. Tendons were weighed and collagen quantification was evaluated by hydroxyprolin content. RESULTS: Significant reduction in collagen content on day 7, 14 and 28 was related to control experiment to normal tendon (7 days, p<0.01; 14 e 28 days, p<0.05). CONCLUSION: The experimental model has been effective and available to be used to study Achilles tendon healing.(AU)

Intestinal healing in rats submitted to ethanol ingestion / Cicatrização intestinal em ratos submetidos à ingestão de etanol

PURPOSE: To study the effect of alcoholism on intestinal healing and postoperative complications in rats METHODS: One hundred and sixty rats were divided into two groups: control and treated. The control group received water and the treated group 30 percent ethanol. After 180 days, colotomy with anastomosis were performed. After, the groups were divided into four subgroups: 20 rats for study at the following moments: 4th, 7th, 14th and 21st postoperative. The analyzed parameters were: weight gain, breaking strength, tissue hydroxyproline, postoperative complications and histopathological study RESULTS: Weight gain was greater in the control group (p<0.05). When all the subgroups were clustered, breaking strength was significantly greater in the control (p<0.05). Histopathology and hydroxyproline dosage did not show differences. There were five surgical site infections in the treated group while the control group showed two (p>0.05). Nine fistulas occurred in the treated group whereas the control group two (p<0.05). There were three deaths in the control group and seven in the treated group (p>0.05). CONCLUSIONS: Treated group undergo a malnutrition process that is revealed by lower weight gain. Impaired intestinal healing as indicated by smaller breaking strength. There were a larger number of postoperative complications in the treated animals.(AU)

OBJETIVO: Estudar o efeito do alcoolismo no processo de cicatrização intestinal e suas complicações pós-operatórias em ratos. MÉTODOS: Cento e sessenta ratos foram divididos em dois grupos: tratado e controle. O controle recebeu água, enquanto o tratado etanol a 30 por cento. Após 180 dias foram realizadas colotomia, seguida de anastomose. Após os animais foram divididos em quatro subgrupos de 20 ratos para estudo nos seguintes momentos: 4º, 7º, 14º e 21º pós-operatório. Os parâmetros analisados foram: ganho de peso, força de ruptura, hidroxiprolina tecidual, complicações pós-operatórias e estudo histopatológico. RESULTADOS: O ganho de peso foi superior no grupo controle (p<0,05). Após agrupamento dos momentos a força de ruptura foi superior no controle (p<0,05). Não houve diferença quanto à histopatologia e hidroxiprolina. Houve cinco infecções de incisão no grupo tratado, enquanto no controle ocorreram duas (p>0,05). Houve nove fístulas no grupo tratado, enquanto no controle duas (p<0,05). Ocorreram sete mortes no grupo tratado e apenas três no controle (p>0,05). CONCLUSÕES: No grupo tratado ocorreu um processo de subnutrição evidenciado pelo menor ganho de peso. Piora na cicatrização intestinal, indicada pela menor força de ruptura. Ocorreu um maior número de complicações pós-operatórias no grupo tratado.(AU)

Características físico-químicas e propriedades tecnológicas da pele de frango / Physico-chemical characteristics and properties technological of chicken skin

Este trabalho teve como objetivo extrair material colagenoso da pele do peito de frango e determinar sua composição físico-química, teor de hidroxiprolina, colágeno, capacidade de retenção de água e de emulsificação. O material colagenoso apresentou em base seca 55,03 g de proteína em 100g de amostra, 14,46g de lipídeos em 100g de amostra, 1,94g de cinzas em 100g de amostra, 2,76g hidroxiprolina em 100g de amostra, 22,08g de colágeno em 100g de amostra, 11,72 de capacidade de retenção de água e 41,67 por cento de capacidade de emulsificação. O material colagenoso apresentou, mediante análises físico-químicas, capacidade de retenção de água e emulsificação, potencial para ser utilizado na indústria de alimentos.(AU)

The aiming of this work is to extract collagenous material from chicken skin and to determine physico-chemical, hidroxyproline and collagen composition. The results were 55,03 g/100g protein (d.b), 14,46 g! 100g lipids (d.b), 1,94 g/100g ash; 2,76 g/1OOghidroxyproline and 22,08 g/100g collagen, 11,72 water retention capacity and 41,67% emulsification capacity from chicken skin. Considering physical chemical properties the collagenous material can be used as an alternative on food industries. (AU)

Quantificação da hidroxiprolina como índice de qualidade de salsicha comercializada em Belo Horizonte-MG

The purpose of this work was to estimate the collagen content of frankfurters by hydroxyproline quantification. Frankfurters were produced at small, medium and large plants, according to the target market (local, regional and national). Determination of hydroxyproline was made by colorimetric method. The results showed that the frankfurters had different standards in the manufacturing, since the collagen content was statistically different. Samples from the medium size industry presented the lowest collagen content (0.45%), followed by samples from the large size plant (0.64%). Frankfurters from the small size industry had the highest collagen content (0.89%). These data showed that this analytical procedure can be used for quality classification of frankfurter based on collagen content.

O presente estudo teve como objetivo estimar o teor de colágeno em salsichas mediante a quantificação da hidroxiprolina. Amostras de salsichas produzidas por indústrias de pequeno, médio e grande porte, classificadas em função do mercado atendido, foram analisadas quanto ao seu conteúdo em hidroxiprolina, por técnica colorimétrica. O teor de colágeno foi significativamente diferente, baseado na quantidade de hidroxiprolina presente nas amostras de salsicha. As amostras que apresentaram o menor teor de colágeno foram as da indústria de médio porte (0,45%), seguida daquelas da indústria de grande porte (0,64%). As salsichas da indústria de pequeno porte apresentaram o maior teor de colágeno (0,89%). Esses resultados mostram que esse teste pode ser utilizado para classificação qualitativa de salsichas, em função dos teores de colágeno.