Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 21
Filtrar
Mais filtros

Intervalo de ano de publicação
1.
Rev. bras. reprod. anim ; 46(2): 84-90, Abril-Junho 2022. graf, tab
Artigo em Português | VETINDEX | ID: biblio-1378064

Resumo

O Brasil vive um cenário de grande demanda pelo produto carne, tanto para o mercado interno quanto para o mercado externo. Para atender a esta demanda, há o envolvimento de toda a cadeia da carne, desde os programas de melhoramento genético com o fornecimento de touros melhoradores para tais características, como as centrais de colheita de sêmen desses animais para uso em massa por meio da Inseminação Artificial em Tempo Fixo (IATF). Para que haja sucesso no uso desta biotécnica, é de vital importância a qualidade do produto sêmen aliado às outras características como ambiência e nutrição.(AU)


Brazil is now with a great demand of beef products, for the domestic and exportation markets. To delivery the demand, the entire meat chain must be involved, included genetics programs to provide and separate superior bulls, and the collection centers that collect this superior genetics bulls to be used in the Fixed Timed Artificial Insemination (FTAI) biotechnique in a large scale. In order to be successful using this biotechnology, the quality of the semen combined with other characteristics such as ambience and nutrition are extremely important.(AU)


Assuntos
Animais , Masculino , Bovinos , Inseminação Artificial/veterinária , Análise do Sêmen/veterinária , Biotecnologia/tendências , Melhoramento Genético/métodos
2.
Arq. bras. med. vet. zootec. (Online) ; 74(6): 969-982, 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1415367

Resumo

The aim of this study was to assess in vitro sperm characteristics and pregnancies/AI (P/AI) of conventional and sex-sorted semen at timed-AI of suckled, multiparous Nelore cows. All cows (n=348) were submitted to a traditional estradiol/progesterone (P4)-based protocol. At 48h after P4-device removal, the estrous behavior was recorded, and AI was performed with conventional or sex-sorted semen from two bulls. The following sperm assessments were performed: CASA, Hyposmotic Test, sperm morphometry and chromatin structure by TB staining. P/AI were reduced (P<0.001) for sex-sorted compared to conventional semen in cows expressing estrus (27vs47%) or not (11vs.37%). Membrane integrity (Bull1: 30.3±9.6 vs. 52.3±12.4%, P=0.01; Bull2: 24.5±3.0 vs. 48.7±1.6%, P=0.006) and sperm concentration (Bulll: 23.2±0.6 vs. 43.0±0.8x10 sperm/mL, P<0.001; Bull2: 25.1+2.8 VS. 42.1±0.7x10 sperm/mL; P<0.001) were reduced in sex-sorted compared to conventional semen, for both bulls. Total and progressive motility were reduced in sex-sorted semen for Bull1 (TM: 49.7±15.9 vs. 94.9±1.9%, P=0.007; PM: 16.7±3.4 vs. 44.1±13.2%, P=0.009) and no differences were detected for Bull2 (TM: 45.0±17.5 vs. 68.2±19.1%, P=0.098; PM: 12.8±4.7 vs. 30.0±13.0%, P=0.065). Sperm ellipticity from sex-sorted was lower than conventional semen for Bull2 (0.306±0.01 vs. 0.342±0.02, P=0.02) and no difference was detected for Bull1 (0.332±0.01 vs. 0.330±0.01, P=0.55). Reduced in vivo fertility was observed for sex-sorted semen, regardless of estrous behavior. In vitro sperm quality of sex-sorted semen was compromised for both bulls, but differently affected for each sire.


O estudo teve como objetivo avaliar características espermáticas in vitro e a taxa de concepção (TC) de sêmen convencional e sexado em um programa de IATF tradicional de vacas Nelore pós-parto. Todas as vacas (n=348) foram submetidas ao mesmo protocolo de IATF à base de estradiol e de progesterona. Após 48 horas da retirada do implante, foi determinada a expressão de estro dos animais e a IA foi realizada com sêmen convencional e sexado de dois touros Angus. As seguintes características espermáticas foram avaliadas: análise computadorizada do sêmen, teste hiposmótico, morfometria espermática e estrutura cromatinica por meio da coloração com azul de toluidina. A TC foi menor (P<0,001) para sêmen sexado comparado ao convencional, em vacas que expressaram estro (27 vs. 47%) e que não apresentaram estro (11 vs. 37%). A integridade da membrana plasmática (Touro 1: 30,319,6 vs. 52,3+12,4%, P=0,010; Touro 2: 24,5+3,0 vs. 48,7±1,6%, P=0,006) e a concentração espermática (Touro 1: 23,2±0,6 vs. 43,0±0,8x10 sperm/mL, P<0,001; Touro 2: 25,1+2,8 vs. 42,1 +0,7x10'sperm/mL, P<0,001) foram menores no sêmen sexado comparado ao convencional, para ambos os touros. Motilidades total e progressiva foram menores no sêmen sexado comparado ao convencional para o Touro 1 (MT: 49,7±15,9 vs. 94,9±1,9%, P=0,007; MP: 16,7+3,4 vs. 44,1+13,2%, P=0,009), enquanto diferenças não foram detectadas no Touro 2 (MT: 45,0±17,5 vs. 68,2±19,1%, P=0,098; MP: 12,8±4,7 vs. 30,0±13,0%, P=0,065). Elipticidade espermática do sêmen sexado foi menor do que do sêmen convencional no Touro 2 (0,306±0,01 vs. 0,342±0,02, P=0,020), mas não houve diferença no Touro 1 (0,332±0,01 vs. 0,330±0,01, P=0,552). Reduzida fertilidade in vivo foi observada para o sêmen sexado em relação ao convencional, independentemente da expressão de cio das vacas. A qualidade seminal in vitro do sêmen sexado foi comprometida para ambos os touros, mas diferentemente afetada para cada reprodutor.


Assuntos
Animais , Gravidez , Bovinos , Prenhez/fisiologia , Inseminação Artificial/veterinária , Taxa de Gravidez , Análise do Sêmen/veterinária , Espermatozoides , Estro
3.
Acta sci. vet. (Impr.) ; 49: Pub.1802-2021. tab
Artigo em Português | VETINDEX | ID: biblio-1458441

Resumo

Background: In order to reverse the White-lipped peccary decline, besides protecting its habitat and controlling hunting,it is necessary a captive breeding program. There are reports, however, on the low fertility of white-lipped peccary, makingit difficult its reproduction in captivity, making artificial insemination one of the main tools to prevent the loss of geneticdiversity of species kept in captivity. Information on safe methods of anesthesia and the collection of semen should beinvestigated. Therefore, we aimed to compare the effects of the anesthetic protocols acepromazine/ketamine and xylazine/ketamine, as well as electroejaculation protocols, for semen collection in white-lipped peccary (Tayassu pecari).Materials, Methods & Results: Twelve adult male white-lipped peccaries were submitted both to the xylazine/ketamineand acepromazine/ketamine anesthetic protocols. The anesthetic induction time and duration, the degree of muscle relaxation, the time for anesthetic recovery and the quality of the animals’ recovery were evaluated. Additionally, the qualityof the sedation was evaluated based on the animal’s behavior. We also evaluated the effect of drugs on erectile functionsas well as the efficiency of 3 electroejaculation protocols with increasing or fixed voltages (2 to 4 V; 5 to 12 V; 12 V). Theacepromazine/ketamine combination promotes shorter induction time, duration and recovery from anesthesia than thexylazine/ketamine association. There were no differences, however, between the tested anesthetic protocols in relation toheart rate, respiratory rate and temperature. Ejaculate was obtained from only 2 animals when using the xylazine/ketamineprotocol and adoption of stimuli between 5 and 12 V, with 10 stimuli at each voltage. In turn, ejaculate was obtained from4 animals submitted to the acepromazine/ketamine protocol, 3 of them with the adoption of stimuli between 5 and 12 V...


Assuntos
Masculino , Animais , Acepromazina , Anestesia/veterinária , Ketamina , Suínos , Xilazina , Animais Selvagens , Ejaculação , Sêmen
4.
Acta sci. vet. (Online) ; 49: Pub. 1802, 16 abril 2021. tab
Artigo em Português | VETINDEX | ID: vti-762304

Resumo

Background: In order to reverse the White-lipped peccary decline, besides protecting its habitat and controlling hunting,it is necessary a captive breeding program. There are reports, however, on the low fertility of white-lipped peccary, makingit difficult its reproduction in captivity, making artificial insemination one of the main tools to prevent the loss of geneticdiversity of species kept in captivity. Information on safe methods of anesthesia and the collection of semen should beinvestigated. Therefore, we aimed to compare the effects of the anesthetic protocols acepromazine/ketamine and xylazine/ketamine, as well as electroejaculation protocols, for semen collection in white-lipped peccary (Tayassu pecari).Materials, Methods & Results: Twelve adult male white-lipped peccaries were submitted both to the xylazine/ketamineand acepromazine/ketamine anesthetic protocols. The anesthetic induction time and duration, the degree of muscle relaxation, the time for anesthetic recovery and the quality of the animals recovery were evaluated. Additionally, the qualityof the sedation was evaluated based on the animals behavior. We also evaluated the effect of drugs on erectile functionsas well as the efficiency of 3 electroejaculation protocols with increasing or fixed voltages (2 to 4 V; 5 to 12 V; 12 V). Theacepromazine/ketamine combination promotes shorter induction time, duration and recovery from anesthesia than thexylazine/ketamine association. There were no differences, however, between the tested anesthetic protocols in relation toheart rate, respiratory rate and temperature. Ejaculate was obtained from only 2 animals when using the xylazine/ketamineprotocol and adoption of stimuli between 5 and 12 V, with 10 stimuli at each voltage. In turn, ejaculate was obtained from4 animals submitted to the acepromazine/ketamine protocol, 3 of them with the adoption of stimuli between 5 and 12 V...(AU)


Assuntos
Animais , Masculino , Suínos , Anestesia/veterinária , Xilazina , Ketamina , Acepromazina , Animais Selvagens , Ejaculação , Sêmen
5.
Arq. bras. med. vet. zootec. (Online) ; 71(3): 929-938, May-June 2019. tab, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1011296

Resumo

Due to the doubts and questions about the inflammatory reaction caused by chemical castration, this study aimed to use infrared thermography to detect, evaluate and monitor the inflammatory reaction caused by the intratesticular injection of calcium chloride (CaCl2) 20% with lidocaine 1%. For this, thermographic measurements were taken before (M0), 10 minutes (M1), 1 and 6 hours (M2 and M3), for 7 consecutive days (M4 to M10), at 15 (M11), 30 (M12) and 60 (M13) days after intratesticular injection. Additionally, changes to testicular tissue and effects over spermatogenesis were evaluated by andrological exam before (M0) and 60 days (M13) after intratesticular injection. All cats were orchiectomized at M13, and testicles were submitted to histological analysis. CaCl2 (20%) with lidocaine (1%) administration produced testicular tissue damage and interfered with the spermatogenesis in 70% of treated cats without exacerbating the inflammatory reaction or impairing the cat's welfare. It was concluded that thermographic evaluation is a useful, efficient, easy and quick method to diagnose and monitor cat testicular inflammatory reactions.(AU)


Devido a constantes dúvidas e questionamentos sobre a reação inflamatória ocasionada pela castração química, este estudo objetivou o uso da termografia infravermelha para detectar, avaliar e monitorar a reação inflamatória causada pela injeção intratesticular de cloreto de cálcio (CaCl 2 ) 20% associada à lidocaína 1%. Para isso, medidas termográficas foram aferidas antes (M0), 10 minutos (M1), uma e seis horas (M2 e M3), por sete dias consecutivos (M4 a M10), aos 15 (M11), 30 (M12), e 60 (M13) dias após injeção intratesticular, nos grupos tratado e controle. Todos os gatos foram orquiectomizados no M13, e os testículos submetidos à análise histológica. A injeção CaCl 2 a 20% associada com lidocaína a 1% produziu lesão testicular e interferiu na espermatogênese de 70% dos gatos tratados, sem exacerbar a reação inflamatória ou prejudicar o bem-estar do animal. Foi concluído que a avaliação termográfica é uma ferramenta útil, eficiente, rápida e fácil para o diagnóstico e o monitoramento da reação inflamatória em gatos.(AU)


Assuntos
Animais , Masculino , Gatos , Castração/métodos , Castração/veterinária , Análise do Sêmen/métodos , Análise do Sêmen/veterinária , Cloreto de Cálcio , Termografia/veterinária , Lidocaína
6.
Arq. bras. med. vet. zootec. (Online) ; 71(3): 929-938, May-June 2019. tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-25546

Resumo

Due to the doubts and questions about the inflammatory reaction caused by chemical castration, this study aimed to use infrared thermography to detect, evaluate and monitor the inflammatory reaction caused by the intratesticular injection of calcium chloride (CaCl2) 20% with lidocaine 1%. For this, thermographic measurements were taken before (M0), 10 minutes (M1), 1 and 6 hours (M2 and M3), for 7 consecutive days (M4 to M10), at 15 (M11), 30 (M12) and 60 (M13) days after intratesticular injection. Additionally, changes to testicular tissue and effects over spermatogenesis were evaluated by andrological exam before (M0) and 60 days (M13) after intratesticular injection. All cats were orchiectomized at M13, and testicles were submitted to histological analysis. CaCl2 (20%) with lidocaine (1%) administration produced testicular tissue damage and interfered with the spermatogenesis in 70% of treated cats without exacerbating the inflammatory reaction or impairing the cat's welfare. It was concluded that thermographic evaluation is a useful, efficient, easy and quick method to diagnose and monitor cat testicular inflammatory reactions.(AU)


Devido a constantes dúvidas e questionamentos sobre a reação inflamatória ocasionada pela castração química, este estudo objetivou o uso da termografia infravermelha para detectar, avaliar e monitorar a reação inflamatória causada pela injeção intratesticular de cloreto de cálcio (CaCl 2 ) 20% associada à lidocaína 1%. Para isso, medidas termográficas foram aferidas antes (M0), 10 minutos (M1), uma e seis horas (M2 e M3), por sete dias consecutivos (M4 a M10), aos 15 (M11), 30 (M12), e 60 (M13) dias após injeção intratesticular, nos grupos tratado e controle. Todos os gatos foram orquiectomizados no M13, e os testículos submetidos à análise histológica. A injeção CaCl 2 a 20% associada com lidocaína a 1% produziu lesão testicular e interferiu na espermatogênese de 70% dos gatos tratados, sem exacerbar a reação inflamatória ou prejudicar o bem-estar do animal. Foi concluído que a avaliação termográfica é uma ferramenta útil, eficiente, rápida e fácil para o diagnóstico e o monitoramento da reação inflamatória em gatos.(AU)


Assuntos
Animais , Masculino , Gatos , Castração/métodos , Castração/veterinária , Análise do Sêmen/métodos , Análise do Sêmen/veterinária , Cloreto de Cálcio , Termografia/veterinária , Lidocaína
7.
Rev. Ciênc. Agrovet. (Online) ; 17(4): 603-607, 2018. tab
Artigo em Português | VETINDEX | ID: biblio-1488265

Resumo

This study aims to qualitatively and quantitatively characterize the semen of tambaqui (Colossoma macropomum) during the breeding season. Three samplings were made at regular intervals of approximately 30 days in the 2012/2013 breeding season. Ten adult males of at least three years old age were selected. To collect the semen, hormonal induction was performed with carp pituitary extract, and extraction occurred by abdominal massage. After collection, the semen was subjected to evaluation of motility, vigor, lifetime, ejaculate volume, and sperm concentration. Analysis of variance (ANOVA) and Tukey test were applied on quantitative variables, while the Freedman test was used with 0.05 significance on qualitative variables. No significant differences (p>0.05) were observed for most parameters evaluated. Sperm concentration significantly varied between samples (14.5, 3.7, and 8.3x109 sperm/mL) and lifetime decreased significantly between the second and third samples (267.8 e 98.5 seconds, respectively).


Objetivou-se com o presente trabalho caracterizar qualitativamente e quantitativamente o sêmen de tambaqui durante o período reprodutivo. Foram realizadas três coletas, com intervalos regulares de aproximadamente 30 dias no período reprodutivo de 2012/2013. Foram utilizados dez reprodutores de no mínimo três anos de idade. Para a coleta de sêmen foi realizada indução hormonal com extrato de hipófise de carpa, e a extração do sêmen ocorreu por massagem abdominal. Após coleta, o sêmen foi submetido a avaliações de motilidade, vigor, tempo de vida, volume do ejaculado e concentração espermática. As variáveis quantitativas foram submetidas à análise de variância (ANOVA) e teste de Tukey, e as variáveis qualitativas ao teste de Friedman, com significância de 0,05. Não foram observadas diferenças significativas (p>0,05) para a maioria dos parâmetros avaliados. A concentração espermática variou significativamente entre as coletas (14,5, 3,7, e 8,3x109 espermatozoides/mL) e o tempo de vida diminuiu significativamente entre a segunda e terceira coletas (267,8 e 98,5 segundos, respectivamente).


Assuntos
Masculino , Animais , Análise do Sêmen/veterinária , Characidae , Espermatozoides , Fenômenos Reprodutivos Fisiológicos , Contagem de Espermatozoides/veterinária
8.
R. Ci. agrovet. ; 17(4): 603-607, 2018. tab
Artigo em Português | VETINDEX | ID: vti-738584

Resumo

This study aims to qualitatively and quantitatively characterize the semen of tambaqui (Colossoma macropomum) during the breeding season. Three samplings were made at regular intervals of approximately 30 days in the 2012/2013 breeding season. Ten adult males of at least three years old age were selected. To collect the semen, hormonal induction was performed with carp pituitary extract, and extraction occurred by abdominal massage. After collection, the semen was subjected to evaluation of motility, vigor, lifetime, ejaculate volume, and sperm concentration. Analysis of variance (ANOVA) and Tukey test were applied on quantitative variables, while the Freedman test was used with 0.05 significance on qualitative variables. No significant differences (p>0.05) were observed for most parameters evaluated. Sperm concentration significantly varied between samples (14.5, 3.7, and 8.3x109 sperm/mL) and lifetime decreased significantly between the second and third samples (267.8 e 98.5 seconds, respectively).(AU)


Objetivou-se com o presente trabalho caracterizar qualitativamente e quantitativamente o sêmen de tambaqui durante o período reprodutivo. Foram realizadas três coletas, com intervalos regulares de aproximadamente 30 dias no período reprodutivo de 2012/2013. Foram utilizados dez reprodutores de no mínimo três anos de idade. Para a coleta de sêmen foi realizada indução hormonal com extrato de hipófise de carpa, e a extração do sêmen ocorreu por massagem abdominal. Após coleta, o sêmen foi submetido a avaliações de motilidade, vigor, tempo de vida, volume do ejaculado e concentração espermática. As variáveis quantitativas foram submetidas à análise de variância (ANOVA) e teste de Tukey, e as variáveis qualitativas ao teste de Friedman, com significância de 0,05. Não foram observadas diferenças significativas (p>0,05) para a maioria dos parâmetros avaliados. A concentração espermática variou significativamente entre as coletas (14,5, 3,7, e 8,3x109 espermatozoides/mL) e o tempo de vida diminuiu significativamente entre a segunda e terceira coletas (267,8 e 98,5 segundos, respectivamente).(AU)


Assuntos
Animais , Masculino , Characidae , Análise do Sêmen/veterinária , Fenômenos Reprodutivos Fisiológicos , Espermatozoides , Contagem de Espermatozoides/veterinária
9.
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1457741

Resumo

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm

10.
Acta sci. vet. (Online) ; 45: 01-07, 2017. tab
Artigo em Português | VETINDEX | ID: vti-691117

Resumo

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed. Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: [...](AU)


Assuntos
Animais , Masculino , Cães , Selênio/administração & dosagem , Vitamina E/administração & dosagem , Análise do Sêmen/veterinária , Administração Oral , Preservação do Sêmen/veterinária , Suplementos Nutricionais , Antioxidantes
11.
Acta sci. vet. (Impr.) ; 45: 01-07, 2017. tab
Artigo em Português | VETINDEX | ID: biblio-1457573

Resumo

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed. Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: [...]


Assuntos
Masculino , Animais , Cães , Administração Oral , Análise do Sêmen/veterinária , Selênio/administração & dosagem , Vitamina E/administração & dosagem , Antioxidantes , Preservação do Sêmen/veterinária , Suplementos Nutricionais
12.
Tese em Português | VETTESES | ID: vtt-213528

Resumo

Em diversas espécies, é reconhecida a importância de técnicas de avaliação da saúde reprodutiva dos machos. Na espécie Mazama gouazoubira, há poucos estudos sobre reprodução. Neste contexto, a avaliação seminal e ultrassonográfica aparecem como importantes ferramentas para analisar o potencial fecundante do macho. Portanto, o objetivo do presente trabalho foi caracterizar a ultrassonografia bidimensional e Doppler testicular e os parâmetros de qualidade seminal, morfometria e ultraestrutura espérmaticas em machos adultos de M. gouazoubira. Foi coletado o sêmen de machos adultos de M. gouazoubira por eletroejaculação (n = 8 ejaculados) e avaliado em cor, aspecto, pH, volume, motilidades total e progressiva, turbilhonamento, vigor, viabilidade, morfologia, morfometria, funcionalidade da membrana, atividade mitocondrial e concentração. Uma alíquota foi separada para análise por microscopia eletrônica de transmissão (MET) e varredura (MEV). Foram mensurados circunferência escrotal e volume testicular. Testículos e epidídimos foram avaliados por ultrassonografia modo B e artéria supratesticular por Triplex Doppler. As imagens bidimensionais foram submetidas à análise computadorizada pelo software ImageJ para determinação do valor numérico de pixels (VNP) e heterogeneidade de pixels (desvio padrão do VNP - DPNP). Baseado na qualidade seminal, os animais foram divididos em 2 grupos (F+ com boa qualidade e F- com baixa qualidade). Os parâmetros de sêmen in natura foram comparados entre três animais diferentes (P < 0,05). Circunferência escrotal, volume testicular, VNP, DPNP e parâmetros de Doppler foram comparados entre os grupos F+ e F- (P < 0,05). Parâmetros de tamanho testicular e de ultrassonografia foram correlacionados (P < 0,05). Experimento 1: Houve diferença entre os indivíduos em relação a funcionalidade de membrana, morfologia e morfometria espermáticas. Os defeitos mais frequentes foram cauda enrolada e peça intermediária encurvada. A análise por MEV demonstraram estruturas perceptíveis apenas por esta técnica, como segmento sub-equatorial e banda serrilhada. A análise por MET revelou características das organelas e composição celular, como a presença de 60 espirais mitocondriais. Experimento 2: O grupo F+ obteve maiores valores de circunferência escrotal, volume testicular, VNP e DPNP testiculares (corte transversal). Não houve diferença entre os grupos nos parâmetros dopplervelociméricos. Houve correlações significativas e positivas entre tamanho testicular (circunferência escrotal e volume testicular) e parâmetros ultrassonográficos (VNP e DPNP testiculares). É possível concluir que diferentes tipos de análise seminal carcaterizam o potencial fértil dos machos de M. gouazoubira. Além disso, as diferenças entre os grupos estão possivelmente ligadas a um desequilíbrio na espermatogênese, sem comprometer o fluxo sanguíneo da artéria supratesticular.


In multiple species, male breeding soundness evaluation is acknowledged as important techniques. In Mazama gouazoubira, there are few studies on reproduction. So, considering semen and ultrasound evaluation can be an important tool to analyze male fertility potential. This study aims to evaluate fresh semen and to measure scrotal circumference, testicular volume, B mode, and spectral Doppler ultrasound parameters in M. gouazoubira. Semen from adult males was collected using electroejaculation (n = 8 ejaculates) and evaluated based on color, aspect, pH, volume, total and progressive motility, mass movement, vigor, viability, morphology, morphometry, membrane integrity, mitochondrial activity and concentration. One aliquot was used for scanning (SEM) and transmission electron microscopy (TEM). Scrotal circumference and testicular volume measured. Testes and epididymis were evaluated using B mode ultrasound and testicular artery blood flow, using Triplex Doppler ultrasound. B mode images were subjected to computerized analysis using ImageJ, generating numerical pixel value (NPV) and pixel heterogeneity (NPV standard deviation PSD). The males were divided in two groups (F+ with good seminal quality and F- with poor seminal quality). Fresh semen parameters were compared amongst three males (P < 0,05). Scrotal circumference, testicular volume, NPV, PSD and Doppler parameters were compared between F+ and F- group (P < 0,05). Testicular size and ultrasound parameters were subjected to correlation test (P < 0,05). Experiment 1: There was difference among males regarding membrane integrity, morphology and morphometry. The most frequent defects were simple bent tail and bowed midpiece. Structures only notable using SEM were observed, such as subequatorial segment and serrated band. TEM revealed organelle characteristics and cell composition, for instance the presence of 60 mitochondria spirals. Experiment 2: F+ group had higher scrotal circumference, testicular volume and testes NPV and PSD (cross-sectional view). No difference between the groups was observed in dopplervelocimetric parameters. There were positive significant correlations between testis size (scrotal circumference and testicular volume) and ultrasound parameters (testes NPV and PSD). Thus, different types of semen analysis help in characterizing fertile potential of M. gouazoubira males. Also, the differences between groups are possibly associated to an imbalance on spermatogenesis that does not compromise supratesticular artery blood flow.

13.
Anim. Reprod. (Online) ; 10(3): 148-159, 2013.
Artigo em Inglês | VETINDEX | ID: biblio-1461059

Resumo

This review summarizes those methods - established and emerging of semen assessment whose outcom e intents revealing its potential fertility and, as a carry over concept, that of the sire whose semen we examined. The review does not, however, focus on the wide display of current techn iques designed to explore specific or multiple sets of sperm attributes essential for fertilization but on two basic con cerns present: the heterogeneity of the sperm suspension and the multitude of attributes required for each spermatozoon to be fertile; concepts that shadow our diagnostic capabilities. The review points out advancements in the exploration of the genome, the transcriptome, and the proteome of both spermatozoa and the seminal plasma which unveil how spermatozoa modulate their own survival an d signal to the environment when displaying degenerative changes. Specific seminal plasma components, both among individuals and portions of the ejaculate, not only relate to survival but also signal differential immune tolerance by the female with a previ ously unattended linkage to fertility. Lastly it foresees how Cytomics, combining novel designed motility analyzers, flow cytometers and enhanced digital imaging shall dominate the landscape of andrological laboratories and enable quick determinations on huge sperm numbers for markers highly relevant to sperm function and hence, for fertility.


Assuntos
Animais , Análise do Sêmen/veterinária , Espermatozoides/citologia , Estro/genética , Bovinos/fisiologia
14.
Anim. Reprod. ; 10(3): 148-159, 2013.
Artigo em Inglês | VETINDEX | ID: vti-8119

Resumo

This review summarizes those methods - established and emerging of semen assessment whose outcom e intents revealing its potential fertility and, as a carry over concept, that of the sire whose semen we examined. The review does not, however, focus on the wide display of current techn iques designed to explore specific or multiple sets of sperm attributes essential for fertilization but on two basic con cerns present: the heterogeneity of the sperm suspension and the multitude of attributes required for each spermatozoon to be fertile; concepts that shadow our diagnostic capabilities. The review points out advancements in the exploration of the genome, the transcriptome, and the proteome of both spermatozoa and the seminal plasma which unveil how spermatozoa modulate their own survival an d signal to the environment when displaying degenerative changes. Specific seminal plasma components, both among individuals and portions of the ejaculate, not only relate to survival but also signal differential immune tolerance by the female with a previ ously unattended linkage to fertility. Lastly it foresees how Cytomics, combining novel designed motility analyzers, flow cytometers and enhanced digital imaging shall dominate the landscape of andrological laboratories and enable quick determinations on huge sperm numbers for markers highly relevant to sperm function and hence, for fertility.(AU)


Assuntos
Animais , Análise do Sêmen/veterinária , Estro/genética , Espermatozoides/citologia , Bovinos/fisiologia
15.
Anim. Reprod. (Online) ; 9(1): 19-26, 2012. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1461671

Resumo

Internal cryoprotectants (dimethyl sulfoxide - DMSO), as well as exter nal ones (glucose) have been of great importance for sperm cryopreservation in freshwater fish. The aim of this study was to evaluate both the fertilization and hatching rates of eggs fertilized with bocachico ( Prochilodus magdalenae ) spermatozoa cryoprese rved in different combinations of DMSO and glucose. Nine treatments were evaluated by a combination of three concentrations of DMSO: 5% (701 mM), 10% (1402 mM), 15% (v /v ; 2103 mM) and three concentr ations of glucose: 5.5% (305 mM), 6% (333 mM), 6.5% (w /v ; 361 mM) . Semen from males obtained by abdominal stripping 6 h after hormonal induction with carp pituitary extract was submitted to each treatment. The semen was frozen in 0.5 m l straws in a nitrogen vapor dry shipper for 30 m in and then in liquid nitrogen ( - 1 96°C). Five days later they were placed in water with a temperature of 60 °C for 8 sec and analyzed. A high total motility (71 .0 ± 7.0%) was observed when DMSO concentration was 10% and glucose was 6%, and a high linearity displacement (62.8 ± 6. 3 %) was observed when DMSO concentration was 5% and glucose was 5.5%. In conclusion, we found that for the purposes of cryopreservation of bocachico spermatozoa, the combination s of 10% DMSO + 5.5 or 6% glucose and 5% DMSO + 5.5 or 6% glucose produced the best re sults in terms of fertilization and hatching rates. This becomes the first report to successfully demonstrate the fertilizing capacity and larvae obtaining capabilities of cryopreserved bocachico semen.


Assuntos
Animais , Análise do Sêmen , Crioprotetores/análise , Espermatozoides/citologia , Glucose/análise , Criopreservação , Peixes/classificação
16.
Anim. Reprod. ; 9(1): 19-26, 2012. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-8554

Resumo

Internal cryoprotectants (dimethyl sulfoxide - DMSO), as well as exter nal ones (glucose) have been of great importance for sperm cryopreservation in freshwater fish. The aim of this study was to evaluate both the fertilization and hatching rates of eggs fertilized with bocachico ( Prochilodus magdalenae ) spermatozoa cryoprese rved in different combinations of DMSO and glucose. Nine treatments were evaluated by a combination of three concentrations of DMSO: 5% (701 mM), 10% (1402 mM), 15% (v /v ; 2103 mM) and three concentr ations of glucose: 5.5% (305 mM), 6% (333 mM), 6.5% (w /v ; 361 mM) . Semen from males obtained by abdominal stripping 6 h after hormonal induction with carp pituitary extract was submitted to each treatment. The semen was frozen in 0.5 m l straws in a nitrogen vapor dry shipper for 30 m in and then in liquid nitrogen ( - 1 96°C). Five days later they were placed in water with a temperature of 60 °C for 8 sec and analyzed. A high total motility (71 .0 ± 7.0%) was observed when DMSO concentration was 10% and glucose was 6%, and a high linearity displacement (62.8 ± 6. 3 %) was observed when DMSO concentration was 5% and glucose was 5.5%. In conclusion, we found that for the purposes of cryopreservation of bocachico spermatozoa, the combination s of 10% DMSO + 5.5 or 6% glucose and 5% DMSO + 5.5 or 6% glucose produced the best re sults in terms of fertilization and hatching rates. This becomes the first report to successfully demonstrate the fertilizing capacity and larvae obtaining capabilities of cryopreserved bocachico semen.(AU)


Assuntos
Animais , Glucose/análise , Análise do Sêmen , Espermatozoides/citologia , Crioprotetores/análise , Peixes/classificação , Criopreservação
17.
Tese em Português | VETTESES | ID: vtt-208577

Resumo

Muitas espécies de primatas neotropicais são ameaçadas de extinção. As biotecnologias da reprodução podem contribuir consistentemente para a conservação das espécies, estando entre elas os bancos de recurso genético. Entretanto, o sucesso no uso de tais tecnologias depende de avanços no conhecimento da biologia reprodutiva básica de uma certa espécie. Durante esse processo, a avaliação da qualidade espermática é de suprema importância. Nós concentramos nosso trabalho em micos-leões-de-cara-dourada (Leontopithecus chrysomelas), mantidos na Fundação Parque Zoológico de São Paulo. Leontopithecus sp. é um alvo importante para o desenvolvimento de técnicas reprodutivas com o propósito da conservação, sendo que todas as espécies do gênero são classificadas como ameaçadas ou criticamente ameaçadas de extinção pela IUCN. Para a avaliação da qualidade espermática em L. chrysomelas, técnicas como o teste de ligação do espermatozoide, e as colorações não fluorescentes para a avaliação de integridade de acrossomo fast-green/rosa bengala, integridade de membrana plasmática eosina-nigrosina e atividade mitocondrial 3,3 diaminobenzidina foram validados para sêmen fresco. Nós também validamos uma técnica de avalição de integridade de acrossomo corante azul de coomassie, para amostras descongeladas devido a inadequação do método utilizado em sêmen fresco. Algumas espécies de primatas neotropicais apresentam variação na qualidade seminal ao longo do ano. Para avaliar a qualidade do sêmen para essa espécies e para verificar se mico-leão-de-cara-dourada possui essa variação, nós colhemos sêmen nas estações de seca e chuva, durante diferentes períodos da estação reprodutiva no intervalo dos períodos conceptivos e no final do segundo período conceptivo. As medições dos testículos foram realizadas previamente a cada colheita de sêmen, e uma diferença significativa foi encontrada no volume testicular total entre as estações, com maiores volumes encontrados durante a estação de seca (p = 0,0011). As amostras colhidas durante a estação de chuva mostraram uma maior porcentagem de motilidade total e integridade de membrana plasmática (p = 0,0149 e p = 0,0279, respectivamente), durante a estação de chuva. Mesmo com essas diferenças, sêmen de ambas as estações apresentaram boa qualidade. A taxa de sucesso das colheitas com a técnica de vibroestimulação peniana foi de 100%, e todas as amostras eram constituídas de somente uma fração coagulada. Apesar de não obtermos a dissolução completa do coágulo, nós pudemos acessar um grande número de espermatozóides pela adição do meio Biggers-Whitten-Whittingham (BWW) ao coágulo por 30 minutos a 37C, e realizamos todas as análises propostas. As mesmas amostras usadas nas avaliações do sêmen fresco foram subsequentemente submetidas à criopreservação usando dois diluidores comerciais diferentes BotuBOV (BB) e Freezing Medium with Glycerol and Gentamicin Test Yolk Buffer (TYB). As amostras descongeladas foram analisadas com os mesmos métodos previamente validados e também foram avaliadas para a suscetibilidade ao estresse oxidativo pela quantificação de substâncias reativas ao ácido tiobarbitúrico (TBARS). Para todos os parâmetros seminais avaliados, não houveram diferenças significativas entre BB e TYB, exceto que o BB apresentou porcentagem de acrossomos íntegros (p = 0.0101) e concentração de TBARS (p = 0.0005) maiores. Apesar dessas diferenças, as amostras descongeladas de ambos diluidores mostraram resultados similares no teste de predição da capacidade fertilizante (ensaio de ligação do espermatozoide). Do nosso trabalho, podemos observar que os métodos propostos foram validados com sucesso para L. chrysomelas. A qualidade do sêmen fresco foi muito alta e ambos diluidores obtiveram sucesso na criopreservação do sêmen.


Many Neotropical primate species are endangered of extinction. Reproductive biotechnologies can consistently contribute to species conservation, being among them the genetic resource banks. However, success in the use of such technologies relies on advances in the knowledge of basic reproductive biology of a given species. During these processes, assessment of sperm quality is of supreme importance. We focused our work in captive golden-headed lion tamarins (Leontopithecus chrysomelas), housed at São Paulo Zoological Park Foundation. Leontopithecus sp. is a very important target for the development of reproductive techniques aiming conservation, as all species from this genus are classified as endangered or critically endangered of extinction by IUCN. For the evaluation of sperm quality in L. chrysomelas, techniques like sperm-binding assay, and the non-fluorescent staining for evaluation of acrosome integrity fast-green/rose-bengal, plasma membrane integrity eosin-nigrosin and mitochondrial activity 3.3 diaminobenzidine were validated for fresh semen. We have also validated an acrosome integrity evaluation technique coomassie blue staining, for thawed samples due to the inadequacy of the method used for fresh semen. Some Neotropical primates species show variation in semen quality over the year. In order to assess semen quality for this species and to verify if golden-headed lion tamarin presents this variation, we collected semen in dry and rainy seasons, during different periods of breeding season in the interval of conceptive periods and in the end of the second conceptive period. Testicular measurements were taken prior each semen collection, and a significant difference was found in total testicular volume between seasons, with major volumes found during dry season (p = 0.0011). Samples collected during rainy season showed higher percentage of total motility and intact plasma membrane (p = 0.0149 and p = 0.0279, respectively), during rainy season. Even with those differences, semen from both seasons presented good quality. The success rate for collection with penile vibrostimulation technique was of 100%, and all samples obtained were constituted by only a coagulated fraction. Despite not achieving coagulum dissolution, we were able to access a high number of spermatozoa by adding Biggers-Whitten-Whittingham (BWW) medium to the coagulum for 30 min at 37C, and performed all proposed analyzes. The same samples used of fresh analyzes were subsequently submitted to cryopreservation using two different commercial extenders BotuBOV (BB) and Freezing Medium with Glycerol and Gentamicin Test Yolk Buffer (TYB). Thawed semen samples were analyzed with the same methods previously validated and were also evaluated for the susceptibility to oxidative stress through quantification of induced thiobartituric acid reactive substances (TBARS). For all seminal parameters assessed, no significant difference was observed between BB and TYB, except that BB presented a higher percentage of intact acrosome (p = 0.0101) and concentration of TBARS (p = 0.0005). Despite those differences, thawed semen from both extenders performed similarly at the prediction of fertilizing capacity (sperm-binding assay). From our work, we observed that the proposed methods were successfully validated for L. chrysomelas. Fresh semen quality was very high and both extenders were successful in cryopreserving semen.

18.
Artigo em Inglês | VETINDEX | ID: vti-733308

Resumo

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm

19.
Artigo em Inglês | VETINDEX | ID: vti-731714

Resumo

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm

20.
Artigo em Inglês | VETINDEX | ID: vti-731223

Resumo

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA