Insights into multiple use water enterobacteria from the Brazilian semi-arid: the first approach by pyrosequencing

Thousands of people living in semi-arid regions face problems of drought and loss of water quality. In addition, high incidence of acute diarrheal diseases related to water consumption has been responsible for a high number of deaths and high economic costs for human health. Many of the diseases can be caused by the presence of enterobacteria in reservoirs that serve for multiple purposes. This study aimed to confirm the presence of potentially harmful bacteria, which was highlighted in other articles, and to reveal non-identified genera by culture-dependent methods and pyrosequencing. Twenty-three genera of the Enterobacteriaceae family were detected, with emphasis on Escherichia genus and confirmation of the presence of species such as Salmonella enterica and Enterobacter cloacae. The abundance of heterotrophic prokaryotes and the physical and chemical data show an expected average for this type of environment due to the numbers historically presented in previous articles. The unprecedented detection of the presence of some potentially pathogenic species can alert and raise awareness of the populations that use stored water in the semi-arid regions. Consequently, as a result of the peculiar characteristics of reservoirs under this climate influence, there is a cosmopolitanism of enterobacteria that may be related to the alarming numbers of infections from Waterborne Diseases.

Insights into multiple use water enterobacteria from the Brazilian semi-arid: the first approach by pyrosequencing

Thousands of people living in semi-arid regions face problems of drought and loss of water quality. In addition, high incidence of acute diarrheal diseases related to water consumption has been responsible for a high number of deaths and high economic costs for human health. Many of the diseases can be caused by the presence of enterobacteria in reservoirs that serve for multiple purposes. This study aimed to confirm the presence of potentially harmful bacteria, which was highlighted in other articles, and to reveal non-identified genera by culture-dependent methods and pyrosequencing. Twenty-three genera of the Enterobacteriaceae family were detected, with emphasis on Escherichia genus and confirmation of the presence of species such as Salmonella enterica and Enterobacter cloacae. The abundance of heterotrophic prokaryotes and the physical and chemical data show an expected average for this type of environment due to the numbers historically presented in previous articles. The unprecedented detection of the presence of some potentially pathogenic species can alert and raise awareness of the populations that use stored water in the semi-arid regions. Consequently, as a result of the peculiar characteristics of reservoirs under this climate influence, there is a cosmopolitanism of enterobacteria that may be related to the alarming numbers of infections from Waterborne Diseases.(AU)

Enterobactérias no leite comercializado informalmente em quatro municípios do sul do Piauí, Brasil / Enterobacteriaceae in raw milk informally marketed in four cities of South of the Piauí, Brazil

Enterobacteriaceae can be present in the environment and in the intestine of warmblooded animals. This study aimed to isolate and identify enterobacteria in informally marketed milk in cities of South of the Piaui. 52 milk samples were acquired at different points of Bom Jesus, Redenção do Gurguéia, Santa Luz and Cristino Castro, Piaui, which were taken into Styrofoam containing ice to food microbiology laboratory of the Federal University of Piauí, in Bom Jesus, where held the bacterial isolation in MacConkey, EMB and XLD. The conclusive biochemical identification and agglutination test was held at the Enterobacteria Laboratory of FIOCRUZ, (Rio de Janeiro-RJ). From the 37 strains, identified the species Escherichia coli, Enterobacter cloacae, Enterobacter gergoviae, Enterobacter aerogenes, Klebsiella pneumoniae, Citrobater freundii, Providencia rettigeri and Serratia marcescens. The informally marketed milk is contaminated by Enterobacteriaceae that can be pathogenic and should be taken preventive measures, such as pasteurization of milk.

Enterobactérias no leite comercializado informalmente em quatro municípios do sul do Piauí, Brasil / Enterobacteriaceae in raw milk informally marketed in four cities of South of the Piauí, Brazil

Enterobacteriaceae can be present in the environment and in the intestine of warmblooded animals. This study aimed to isolate and identify enterobacteria in informally marketed milk in cities of South of the Piaui. 52 milk samples were acquired at different points of Bom Jesus, Redenção do Gurguéia, Santa Luz and Cristino Castro, Piaui, which were taken into Styrofoam containing ice to food microbiology laboratory of the Federal University of Piauí, in Bom Jesus, where held the bacterial isolation in MacConkey, EMB and XLD. The conclusive biochemical identification and agglutination test was held at the Enterobacteria Laboratory of FIOCRUZ, (Rio de Janeiro-RJ). From the 37 strains, identified the species Escherichia coli, Enterobacter cloacae, Enterobacter gergoviae, Enterobacter aerogenes, Klebsiella pneumoniae, Citrobater freundii, Providencia rettigeri and Serratia marcescens. The informally marketed milk is contaminated by Enterobacteriaceae that can be pathogenic and should be taken preventive measures, such as pasteurization of milk.(AU)