Colonization by Streptococcus pneumoniae among children in Porto Velho, Rondônia, Western Brazilian Amazon / Colonização por Streptococcus pneumoniae em crianças na Amazônia Ocidental Brasileira, Porto Velho, Rondônia

Streptococcus pneumoniae is one of the primary pathogens that are associated with acute respiratory infections (ARI) that cause high rates of morbidity and mortality among children under five years of age in developed and developing countries. This study aimed to determine the prevalence of nasopharyngeal colonization, the antimicrobial resistance profile, and the capacity for biofilm formation by S. pneumoniae isolated from children aged 0-6 years with ARI throughout the Porto Velho-RO. A total of 660 swabs were collected from children with ARI. Molecular and biochemical tests were performed to characterize the isolates. The disk-difusion method and the E-test were used for antimicrobial sensitivity testing (TSA). Biofilm formation capacity was assessed using microtiter plate assays, and serotype detection was acheived using polymerase chain reaction (PCR) analyses. The colonization rate for S. pneumoniae was 8.9% (59/660) and exhibited a high prevalence in children under 23 months of age 64.4% (38/59). The observed serotypes were 9V and 19F with frequencies of 1.7% (1/59) and 13.6% (8/59), respectively. The antimicrobial susceptibility test revealed 100% (59/59) sensitivity to vancomycin. In contrast, trimethoprim and oxacillin exhibited high resistance rates of 76.3% (45/59) and 52.5% (31/59), respectively. Of the biofilm-forming isolates, 54.8% (23/42) possessed resistance to some antimicrobials. In this study, S. pneumoniae showed high rates of antimicrobial resistance and the ability to form biofilms, as these are factors that favor bacterial persistence and can cause serious damage to the host.(AU)

Streptococcus pneumoniae é um dos principais patógenos associados a infecções respiratórias agudas (IRAs) que causam altas taxas de morbidade e mortalidade entre crianças menores de cinco anos de idade em países desenvolvidos e em desenvolvimento. Este estudo teve como objetivo determinar a prevalência de colonização da nasofaringe, o perfil de resistência antimicrobiana e a capacidade de formação de biofilme dos S. pneumoniae isolados de crianças de 0 a 6 anos com IRA na cidade de Porto Velho-Rondônia. Um total de 660 swabs foi coletado de crianças com IRA. Testes moleculares e bioquímicos foram realizados para identificar os isolados bacterianos. O método de disco-difusão e o E-test foram utilizados para o teste de sensibilidade antimicrobiana (TSA). A capacidade de formação de biofilme foi avaliada por meio de ensaios em placas de microtitulação e a detecção de sorotipos foi obtida por meio de análises de Reação em Cadeia da Polimerase (PCR). A taxa de colonização por S. pneumoniae foi de 8,9% (59/660) e apresentou alta prevalência em menores de 23 meses de idade 64,4% (38/59). Os sorotipos identificados foram 9V e 19F com frequências de 1,7% (1/59) e 13,6% (8/59) respectivamente. O teste de sensibilidade aos antimicrobianos revelou 100% (59/59) de sensibilidade à vancomicina. Em contraste, trimetoprima e oxacilina apresentaram altas taxas de resistência de 76,3% (45/59) e 52,5% (31/59) respectivamente. Dos isolados formadores de biofilme 54,8% (23/42) possuíam resistência a alguns dos antimicrobianos. Neste estudo, S. pneumoniae apresentou altas taxas de resistência antimicrobiana e capacidade de formar biofilmes, pois são fatores que favorecem a persistência bacteriana e podem causar sérios danos ao hospedeiro.(AU)

Effect of different rearing condition on early age in the maze, open-field, and aversive behaviours in the dairy cow during the first lactation

The aim of the study was to tested hypotheses that the primiparous cows behaviour is impacted by the method of rearing from the second day of life to weaning. 53 Holstein primiparous cows were used. They were reared in different ways during milk nutrition. Calf-heifers were assigned to one of five rearing groups on the second day after having nursed their mothers for 24 hours: H) hutch; HA) hutch, from 7 days pen with an automated drinking machine (ADM); MA) with mother, from 7 days pen with an ADM; MH) with mother, from 7 days hutch; MN) with mother, from 4 days with nursing cows. After weaning (56 days), all heifers were kept in group pens, after calving in free-stall housing. Maze learning ability was tested in the third month of lactation. In the 5th month, cows were observed in the open-field, in the seventh month they were subjected to an aversion test (AT). Group MN was the slowest in solving all tasks and HA the fastest. Differences were recorded among groups in the first-day evaluation (P < 0.01). No differences in open field tests were found. In the AT traversing, the slowest were groups H and HA, the fastest MH and MA (P < 0.01). The time of the first return was different (P < 0.001). On the first day, the most vocalized cows of the MN group (P < 0.05). The results show that the method used to rear heifers may have a significant impact on their maze and aversive behaviour.(AU)

Effect of different rearing condition on early age in the maze, open-field, and aversive behaviours in the dairy cow during the first lactation

The aim of the study was to tested hypotheses that the primiparous cow’s behaviour is impacted by the method of rearing from the second day of life to weaning. 53 Holstein primiparous cows were used. They were reared in different ways during milk nutrition. Calf-heifers were assigned to one of five rearing groups on the second day after having nursed their mothers for 24 hours: H) hutch; HA) hutch, from 7 days pen with an automated drinking machine (ADM); MA) with mother, from 7 days pen with an ADM; MH) with mother, from 7 days hutch; MN) with mother, from 4 days with nursing cows. After weaning (56 days), all heifers were kept in group pens, after calving in free-stall housing. Maze learning ability was tested in the third month of lactation. In the 5th month, cows were observed in the open-field, in the seventh month they were subjected to an aversion test (AT). Group MN was the slowest in solving all tasks and HA the fastest. Differences were recorded among groups in the first-day evaluation (P < 0.01). No differences in open field tests were found. In the AT traversing, the slowest were groups H and HA, the fastest MH and MA (P < 0.01). The time of the first return was different (P < 0.001). On the first day, the most vocalized cows of the MN group (P < 0.05). The results show that the method used to rear heifers may have a significant impact on their maze and aversive behaviour.

Epidemiological study of dengue fever in District Swabi, Khyber Pakhtunkhwa, Pakistan / Estudo epidemiológico da dengue no distrito Swabi, Khyber Pakhtunkhwa, Paquistão

Dengue is a viral disease which is serious health concern from last few decades and the infection transmitted through mosquito bite into human. This study was conducted to carry out prevalence of dengue fever in District Swabi. A total of 196 blood sample were collected from patients with age ranges (0-80 years) having dengue fever on the basis of physical symptoms from Bacha Khan Medical Complex Swabi during August to October 2017. Serological test were performed for detection of IgM, IgG and NS1 (Non structural protein antigen of virus) against dengue. Out of total 196 confirmed dengue cases the most prone gender was male 123(62%) while 73(38%) were female. Among the age groups; 21-30 years group 62 (31.6%) was found the most predominated age group. The higly affected areas in district Swabi were Topi (40.8%) followed by Main Swabi (27%), Maneri (8.2%), Marghuz (6%), Shawa Ada (5.1%), Shah Mansoor (5.1%), Gohati (4.1%), and Chota Lahore (3.6%). Therefore, the health department should take actions by educating the public about basic cleanliness of the environment. The community should be encouraged to participate in the control of such vector based diseases/infections.

A dengue é uma doença viral que é um sério problema de saúde das últimas décadas e a infecção transmitida através da picada de mosquito em humanos. Este estudo foi realizado para realizar a prevalência da dengue no distrito de Swabi. Foram coletadas 196 amostras de sangue de pacientes com faixa etária (0 a 80 anos) com dengue com base nos sintomas físicos do Complexo Médico Bacha Khan de Swabi, no período de agosto a outubro de 2017. Foram realizados testes sorológicos para detecção de IgM, IgG e NS1 (antígeno proteico não estrutural do vírus) contra a dengue. Do total de 196 casos confirmados de dengue, o sexo mais propenso foi o masculino 123 (62%), enquanto 73 (38%) eram do sexo feminino. Entre as faixas etárias; A faixa etária de 21 a 30 anos 62 (31,6%) foi a faixa etária mais predominante. As áreas altamente afetadas no distrito de Swabi foram Topi (40,8%), seguidas por Main Swabi (27%), Maneri (8,2%), Marghuz (6%), Shawa Ada (5,1%), Shah Mansoor (5,1%), Gohati (4,1%) e Chota Lahore (3,6%). Portanto, o departamento de saúde deve tomar ações educando o público sobre a limpeza básica do meio ambiente. A comunidade deve ser incentivada a participar do controle de tais doenças / infecções baseadas em vetores.

Teeth wear of piglets in lactation phase: performance and injuries / Desgaste dentário de leitões em fase de lactação: aspectos de desempenho e lesões

Piglets are born with a total of eight teeth and on the second day of life, teeth wear management is carried out to avoid lesions on the sows' ceilings and on the faces of the piglets. For this, an electric wearer is used that only wears the apex of the teeth. In this experiment, a comparison of twelve litters divided into: a control group (CG) was performed, which the intact teeth were kept; with the management of tooth wear and treatment group (TG),. The animals were followed from the first day of life until weaning. The weighing was carried out on the respective days of life: second, seventh, fourteenth and twenty-first. It was observed that the fights between piglets and for the ceilings did not present significant difference, on the other hand the injuries caused by the teeth and the dirt injuries had significant difference with greater presence in the group without wear. In the comparison of the piglets’ daily weight gain, there was noted that no significant difference between two groups was observed, which can state that the non-wear of the teeth does not interfere in the daily weight gain, however it was concluded that the animals without wear (CG) had a higher incidence of dirt lesions.

Os leitões nascem com total de oito dentes e no segundo dia de vida é realizado o manejo de desgaste de dentes para evitar lesões nos tetos das matrizes e nas faces dos leitões. Para isso, usa-se desgastador elétrico, que faz o desgaste somente do ápice dos dentes. Neste experimento, realizou-se a comparação de doze leitegadas divididas em: grupo controle (GC), cujos animais se mantiveram com os dentes íntegros; e grupo tratamento (GT), cujos leitões tiveram o desgaste de dentes. Os animais foram acompanhados do primeiro dia de vida até a desmame, quando ocorreu a pesagem nos seguintes dias de vida: segundo, sétimo, décimo quarto e vigésimo primeiro. Observou-se que as brigas entre leitões e pelos tetos não apresentaram diferença significativa, por outro lado, as lesões causadas pelos dentes e as lesões de sujeira tiveram diferença significativa, com maior presença no GC. A comparação do ganho de peso diário (GPD) dos leitões dos dois grupos revelou que não houve diferença significativa, por isso se pode afirmar que o não desgaste dos dentes não interferiu no GPD, entretanto, foi possível concluir que os animais sem desgaste (GC) apresentaram maior ocorrência de lesões de sujeira.

Silagem da parte aérea da mandioca como um alimento alternativo na dieta de vacas em lactação ­ revisão de literatura / Silage of the aerial part of the cassava as an alternative feed in the diet of lactating cows - literature review / Ensilado de la parte aérea de la yuca como alimento alternativo en la dieta de vacas lactantes - revisión de literatura

O setor de nutrição e produção animal busca constantemente por fontes de alimentos que oportunizem os fatores custo e benefício para a elaboração de formulação de dietas. A mandioca é um alimento mundialmente cultivado e apresenta diversos subprodutos ao decorrer de sua produção com excelente característica energética, que podem ser utilizados, como é o caso de sua parte aérea, em substituição à silagem de milho na dieta de vacas leiteiras. Porém, sua inclusão na dieta de vacas em lactação deve ser realizada com cautela, necessitando de estudos que viabilizem o processamento e as formulações em quantidades aceitáveis, tendo em vista seus fatores antinutricionais.(AU)

The animal nutrition and production industry is constantly looking for feed sources that are both cost efficient and beneficial for the preparation of diet formulation. Cassava is cultivated worldwide and has several by-products during its production, with excellent energetic characteristics, such as in the aerial part of the plant, that can be used to replace corn silage in the diet of dairy cows. However, its inclusion in the diet of lactating cows must be carried out with caution, with the need of further studies on the feasibility of its processing and the development of formulations in acceptable amounts, taking into consideration its anti-nutritional factors.(AU)

El sector de nutrición y producción animal busca constantemente por fuentes de alimentos que proporcionen factores de costo y beneficio para la preparación de formulaciones dietéticas. La yuca es un alimento cultivado a nivel mundial y presenta varios subproductos durante su producción, con excelentes características energéticas, que pueden ser utilizados, como en el caso de su parte aérea, en reemplazo al ensilaje de maíz en la dieta de vacas lecheras. Sin embargo, su inclusión en la dieta de vacas lactantes debe realizarse con cautela, necesitando de estudios que viabilicen el procesamiento y las formulaciones en cantidades aceptables, en vista de sus factores anti nutricionales.(AU)

Silagem da parte aérea da mandioca como um alimento alternativo na dieta de vacas em lactação ­ revisão de literatura / Silage of the aerial part of the cassava as an alternative feed in the diet of lactating cows - literature review / Ensilado de la parte aérea de la yuca como alimento alternativo en la dieta de vacas lactantes - revisión de literatura

O setor de nutrição e produção animal busca constantemente por fontes de alimentos que oportunizem os fatores custo e benefício para a elaboração de formulação de dietas. A mandioca é um alimento mundialmente cultivado e apresenta diversos subprodutos ao decorrer de sua produção com excelente característica energética, que podem ser utilizados, como é o caso de sua parte aérea, em substituição à silagem de milho na dieta de vacas leiteiras. Porém, sua inclusão na dieta de vacas em lactação deve ser realizada com cautela, necessitando de estudos que viabilizem o processamento e as formulações em quantidades aceitáveis, tendo em vista seus fatores antinutricionais.(AU)

The animal nutrition and production industry is constantly looking for feed sources that are both cost efficient and beneficial for the preparation of diet formulation. Cassava is cultivated worldwide and has several by-products during its production, with excellent energetic characteristics, such as in the aerial part of the plant, that can be used to replace corn silage in the diet of dairy cows. However, its inclusion in the diet of lactating cows must be carried out with caution, with the need of further studies on the feasibility of its processing and the development of formulations in acceptable amounts, taking into consideration its anti-nutritional factors.(AU)

El sector de nutrición y producción animal busca constantemente por fuentes de alimentos que proporcionen factores de costo y beneficio para la preparación de formulaciones dietéticas. La yuca es un alimento cultivado a nivel mundial y presenta varios subproductos durante su producción, con excelentes características energéticas, que pueden ser utilizados, como en el caso de su parte aérea, en reemplazo al ensilaje de maíz en la dieta de vacas lecheras. Sin embargo, su inclusión en la dieta de vacas lactantes debe realizarse con cautela, necesitando de estudios que viabilicen el procesamiento y las formulaciones en cantidades aceptables, en vista de sus factores anti nutricionales.(AU)

Teeth wear of piglets in lactation phase: performance and injuries / Desgaste dentário de leitões em fase de lactação: aspectos de desempenho e lesões

Piglets are born with a total of eight teeth and on the second day of life, teeth wear management is carried out to avoid lesions on the sows' ceilings and on the faces of the piglets. For this, an electric wearer is used that only wears the apex of the teeth. In this experiment, a comparison of twelve litters divided into: a control group (CG) was performed, which the intact teeth were kept; with the management of tooth wear and treatment group (TG),. The animals were followed from the first day of life until weaning. The weighing was carried out on the respective days of life: second, seventh, fourteenth and twenty-first. It was observed that the fights between piglets and for the ceilings did not present significant difference, on the other hand the injuries caused by the teeth and the dirt injuries had significant difference with greater presence in the group without wear. In the comparison of the piglets daily weight gain, there was noted that no significant difference between two groups was observed, which can state that the non-wear of the teeth does not interfere in the daily weight gain, however it was concluded that the animals without wear (CG) had a higher incidence of dirt lesions.(AU)

Os leitões nascem com total de oito dentes e no segundo dia de vida é realizado o manejo de desgaste de dentes para evitar lesões nos tetos das matrizes e nas faces dos leitões. Para isso, usa-se desgastador elétrico, que faz o desgaste somente do ápice dos dentes. Neste experimento, realizou-se a comparação de doze leitegadas divididas em: grupo controle (GC), cujos animais se mantiveram com os dentes íntegros; e grupo tratamento (GT), cujos leitões tiveram o desgaste de dentes. Os animais foram acompanhados do primeiro dia de vida até a desmame, quando ocorreu a pesagem nos seguintes dias de vida: segundo, sétimo, décimo quarto e vigésimo primeiro. Observou-se que as brigas entre leitões e pelos tetos não apresentaram diferença significativa, por outro lado, as lesões causadas pelos dentes e as lesões de sujeira tiveram diferença significativa, com maior presença no GC. A comparação do ganho de peso diário (GPD) dos leitões dos dois grupos revelou que não houve diferença significativa, por isso se pode afirmar que o não desgaste dos dentes não interferiu no GPD, entretanto, foi possível concluir que os animais sem desgaste (GC) apresentaram maior ocorrência de lesões de sujeira.(AU)

Silagem da parte aérea da mandioca como um alimento alternativo na dieta de vacas em lactação ­ revisão de literatura / Silage of the aerial part of the cassava as an alternative feed in the diet of lactating cows - literature review / Ensilado de la parte aérea de la yuca como alimento alternativo en la dieta de vacas lactantes - revisión de literatura

O setor de nutrição e produção animal busca constantemente por fontes de alimentos que oportunizem os fatores custo e benefício para a elaboração de formulação de dietas. A mandioca é um alimento mundialmente cultivado e apresenta diversos subprodutos ao decorrer de sua produção com excelente característica energética, que podem ser utilizados, como é o caso de sua parte aérea, em substituição à silagem de milho na dieta de vacas leiteiras. Porém, sua inclusão na dieta de vacas em lactação deve ser realizada com cautela, necessitando de estudos que viabilizem o processamento e as formulações em quantidades aceitáveis, tendo em vista seus fatores antinutricionais.(AU)

The animal nutrition and production industry is constantly looking for feed sources that are both cost efficient and beneficial for the preparation of diet formulation. Cassava is cultivated worldwide and has several by-products during its production, with excellent energetic characteristics, such as in the aerial part of the plant, that can be used to replace corn silage in the diet of dairy cows. However, its inclusion in the diet of lactating cows must be carried out with caution, with the need of further studies on the feasibility of its processing and the development of formulations in acceptable amounts, taking into consideration its anti-nutritional factors.(AU)

El sector de nutrición y producción animal busca constantemente por fuentes de alimentos que proporcionen factores de costo y beneficio para la preparación de formulaciones dietéticas. La yuca es un alimento cultivado a nivel mundial y presenta varios subproductos durante su producción, con excelentes características energéticas, que pueden ser utilizados, como en el caso de su parte aérea, en reemplazo al ensilaje de maíz en la dieta de vacas lecheras. Sin embargo, su inclusión en la dieta de vacas lactantes debe realizarse con cautela, necesitando de estudios que viabilicen el procesamiento y las formulaciones en cantidades aceptables, en vista de sus factores anti nutricionales.(AU)

Use of pyridoxine hydrochloride in the interruption of lactation in female dogs with pseudopregnancy

The aim of this research was to evaluate the use of pyridoxine hydrochloride and its associated side effects in the treatment of pseudopregnancy in female dogs. A total of 40 female dogs, with no defined breed, in non-gestational diestrus, with clinical complaint of milk production were selected. The female dogs were divided into four experimental groups of 10 animals each, treated orally for 20 days with 10mg/kg/day (G1) and 50mg/kg/day (G2) of pyridoxine hydrochloride (vitamin B6), 5μg/kg/day of cabergoline (G3), and with a placebo, in the case of the control group (G4). The effects of the treatments on milk production were investigated, as well as possible systemic side effects, macroscopic uterine and ovarian alterations, and uterine histology. During the investigated period, G2 and G3 were equally efficient (P>0.05) in lactation suppression, differing (P>0.05) from the other groups. There were no systemic side effects or uterine changes associated with administration of the studied drug. Vitamin B6 (50mg/kg) has shown to be a safe and economically viable alternative for lactation interruption in female dogs with pseudopregnancy.(AU)

Dynamics of dairy calf rearing systems during the suckling period: a conceptual model / Dinâmica do sistema de criação de bezerras no período de aleitamento: modelo conceitual

Behavioral studies help to understand the needs of animals and serve as a basis to justify  welfare criteria on farms. This study used the systems dynamics to identify causal loops that influence the behavior of dairy calves during suckling when kept in different housing systems (individual or collective in the presence or absence of a tactile stimulus). The results showed that the conceptual model of dairy calf development consists of three subsystems : (a) subsystem of growth with three reinforcing loops linked to the weight of the calves; (b) subsystem of social development with two reinforcing loops associated with calf learning and two balancing loops associated with reactivity, and (c) subsystem of thermoregulation with three balancing loops associated with body temperature and one reinforcing loop associated with thermal stress. In conclusion, conceptual modeling provided additional information  on the behavior of dairy calves by making explicit the interconnections between the subsystems that comprise the overall calf development system.(AU)

O estudo do comportamento ajuda a entender as necessidades dos animais e serve de base para justificar os critérios de bem-estar nas fazendas. Este trabalho utilizou a dinâmica de sistema para encontrar os ciclos causais que influenciam os comportamentos das bezerras leiteiras durante a fase de aleitamento, mantidas em diferentes sistemas de criação (individual ou coletivo com presença ou ausência de estímulo tátil). Como resultado da modelagem, o modelo conceitual de desenvolvimento de bezerras leiteiras possui três subsistemas: (a) subsistema de crescimento, onde existem três ciclos de reforço ligados ao peso das bezerras; (b) subsistema de desenvolvimento social com dois ciclos de reforço associados à aprendizagem das bezerras e dois ciclos de balanço associados à reatividade; e (c) subsistema de termorregulação com três ciclos de balanço associados à temperatura corporal e um ciclo de reforço associado ao estresse térmico. A modelagem conceitual proporcionou maiores informações sobe o comportamento de bezerras leiteiras por tornarem explicitas as integrações entre os subsistemas que compõem o sistema de desenvolvimento geral de bezerras.(AU)

Dynamics of dairy calf rearing systems during the suckling period: a conceptual model / Dinâmica do sistema de criação de bezerras no período de aleitamento: modelo conceitual

Behavioral studies help to understand the needs of animals and serve as a basis to justify  welfare criteria on farms. This study used the systems dynamics to identify causal loops that influence the behavior of dairy calves during suckling when kept in different housing systems (individual or collective in the presence or absence of a tactile stimulus). The results showed that the conceptual model of dairy calf development consists of three subsystems : (a) subsystem of growth with three reinforcing loops linked to the weight of the calves; (b) subsystem of social development with two reinforcing loops associated with calf learning and two balancing loops associated with reactivity, and (c) subsystem of thermoregulation with three balancing loops associated with body temperature and one reinforcing loop associated with thermal stress. In conclusion, conceptual modeling provided additional information  on the behavior of dairy calves by making explicit the interconnections between the subsystems that comprise the overall calf development system.

O estudo do comportamento ajuda a entender as necessidades dos animais e serve de base para justificar os critérios de bem-estar nas fazendas. Este trabalho utilizou a dinâmica de sistema para encontrar os ciclos causais que influenciam os comportamentos das bezerras leiteiras durante a fase de aleitamento, mantidas em diferentes sistemas de criação (individual ou coletivo com presença ou ausência de estímulo tátil). Como resultado da modelagem, o modelo conceitual de desenvolvimento de bezerras leiteiras possui três subsistemas: (a) subsistema de crescimento, onde existem três ciclos de reforço ligados ao peso das bezerras; (b) subsistema de desenvolvimento social com dois ciclos de reforço associados à aprendizagem das bezerras e dois ciclos de balanço associados à reatividade; e (c) subsistema de termorregulação com três ciclos de balanço associados à temperatura corporal e um ciclo de reforço associado ao estresse térmico. A modelagem conceitual proporcionou maiores informações sobe o comportamento de bezerras leiteiras por tornarem explicitas as integrações entre os subsistemas que compõem o sistema de desenvolvimento geral de bezerras.

Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children

This study aimed to evaluate the cytotoxicity and genotoxicity and determine the LC50 concentration of powdered infant formulas widely marketed in South American countries. To this, milk samples, called as A, B, C and D, were analyzed in root meristem cells of Allium cepa, at concentrations of 0.075; 0.15 and 0.30 g mL-1, for 24 and 48 hours; and through cell viability in culture of normal line cells, via MTT test, for 24 hours, in the concentrations 0.018; 0.0375; 0.075 and 0.15 g mL-1. In A. cepa, all dairy products in the three concentrations caused significant inhibition of cell division in the meristems within the first 24 hours of exposure. In the in vitro evaluation, all milk formulas at 0.15 g mL-1, as well as milk A at a concentration of 0.037 g mL-1, C at 0.075 g mL-1 and D at 0.037 g mL-1, significantly reduced the cellular viability of the cell culture exposed to the foods studied, being potentially toxic. The milk A was considered the most toxic, with LC50 of 0.031 g mL-1, and B as the least toxic, with LC50 of 0.15 g mL-1. Therefore, the milk evaluated caused significant instability in cells of the test systems used and were characterized as cytotoxic.

Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children

This study aimed to evaluate the cytotoxicity and genotoxicity and determine the LC50 concentration of powdered infant formulas widely marketed in South American countries. To this, milk samples, called as A, B, C and D, were analyzed in root meristem cells of Allium cepa, at concentrations of 0.075; 0.15 and 0.30 g mL-1, for 24 and 48 hours; and through cell viability in culture of normal line cells, via MTT test, for 24 hours, in the concentrations 0.018; 0.0375; 0.075 and 0.15 g mL-1. In A. cepa, all dairy products in the three concentrations caused significant inhibition of cell division in the meristems within the first 24 hours of exposure. In the in vitro evaluation, all milk formulas at 0.15 g mL-1, as well as milk A at a concentration of 0.037 g mL-1, C at 0.075 g mL-1 and D at 0.037 g mL-1, significantly reduced the cellular viability of the cell culture exposed to the foods studied, being potentially toxic. The milk A was considered the most toxic, with LC50 of 0.031 g mL-1, and B as the least toxic, with LC50 of 0.15 g mL-1. Therefore, the milk evaluated caused significant instability in cells of the test systems used and were characterized as cytotoxic.(AU)

Occurrence of gastrointestinal parasitic diseases of swine in different production phases in commercial pig farms from the State of Santa Catarina, southern Brazil / [Ocorrência de parasitoses gastrointestinais dos suínos, nas diferentes fases de produção, em granjas comerciais de Santa Catarina, Brasil]

Gastrointestinal parasites are important, since they can cause a decrese in the performance of swine. The occurrence of parasites in pig farms can create economic losses such as reduction in the value of carcasses and increasing expenses with medicines and veterinary assistance. This study aimed at investigating the occurrence of intestinal parasites in different phases of production reared pigs in intensive prodution systems in the western region of the State of Santa Catarina. A total of 403 fecal samples were analyzed, of which 51.86% were positive for one or more parasites in different phases of production. Finishing pigs had the highest prevalence of parasites (60.19%) followed by nursery pigs (55.44%), pigs in breading sows (50.49%), and suckling piglets (40.81%). Strongylida parasites had higher occurrence (28.78%), and were also the most frequent in the finishing, breading sows, and nursery phases. Coccidia were more occurrent in suckling piglets. This study highlights the need of implementing suitable antiparasitic control measures in all phases of swine production associated with a surveillance system for the diagnosis of endoparasite infection in pigs. Our results demonstrate that endoparasites are highly occurring in pigs, even in intensive system animals on technified farms.(AU)

Parasitas gastrointestinais são importantes, pois podem causar uma diminuição no desempenho dos suínos. A ocorrência de parasitas em granjas de suínos pode gerar perdas econômicas, como redução no valor das carcaças e aumento de gastos com medicamentos e assistência veterinária. Este estudo teve como objetivo investigar a ocorrência de parasitas intestinais nas diferentes fases de produção de suínos criados em sistemas intensivos, na região oeste do estado de Santa Catarina. Foram analisadas 403 amostras fecais, das quais 51,86% foram positivas para um ou mais parasitas nas diferentes fases de produção. Os suínos de terminação apresentaram a maior prevalência de parasitas (60,19%), seguidos por suínos de creche (55,44%), suínos em gestação (50,49%) e leitões em lactação (40,81%). Parasitas da ordem Strongylida tiveram maior ocorrência (28,78%) e também foram os mais frequentes nos estágios de terminação, reprodução e creche. Coccídios foram mais ocorrentes em leitões lactentes. Este estudo destaca a necessidade de implementar medidas de controle antiparasitário adequadas em todas as fases da produção suína, associadas a um sistema de vigilância para o diagnóstico de infecção por endoparasitas em suínos. Os resultados demonstram que os endoparasitas são altamente ocorrentes em suínos, mesmo em animais de criação intensiva em granjas tecnificadas.(AU)

Vinasse in the diet of lactating sows and its effect on litter

The aim of this study was to verify the impact of adding vinasse as an acidifier in sows diet during the lactating period and its effect on litter. A total of 14 agroceres females were used from the first day of lactation until weaning. The vinasse was obtained in a mill near the experimental facility and was stored in sterilized barrels. Animals were distributed in a completely randomized experimental design into two treatments: control liquid diet and liquid diet with the inclusion of vinasse in each feed supply of the day. The diets were supplied four times a day, containing a mixture of ½ L of vinasse and ½ L of water. Feed leftovers were weighted daily. Feed intake was used to evaluate sows performance. The piglets were weighted every week after birth until weaning in order to obtain weight gain. Vinasse inclusion showed an increase (p < 0.05) in feed intake of lactating sows, improving diet palatability. Inclusion of acidifier in the diet did not show (p > 0.05) difference in weight gain of piglets. Vinasse consumption by sows did not influence litter performance. The inclusion of vinasse in the diet of lactating sows is beneficial because increases feed consumption.(AU)

Vinasse in the diet of lactating sows and its effect on litter

The aim of this study was to verify the impact of adding vinasse as an acidifier in sows diet during the lactating period and its effect on litter. A total of 14 agroceres females were used from the first day of lactation until weaning. The vinasse was obtained in a mill near the experimental facility and was stored in sterilized barrels. Animals were distributed in a completely randomized experimental design into two treatments: control liquid diet and liquid diet with the inclusion of vinasse in each feed supply of the day. The diets were supplied four times a day, containing a mixture of ½ L of vinasse and ½ L of water. Feed leftovers were weighted daily. Feed intake was used to evaluate sows’ performance. The piglets were weighted every week after birth until weaning in order to obtain weight gain. Vinasse inclusion showed an increase (p 0.05) difference in weight gain of piglets. Vinasse consumption by sows did not influence litter performance. The inclusion of vinasse in the diet of lactating sows is beneficial because increases feed consumption.

Principal component analysis of pre-weaning growth traits in Santa Inês lambs / Análise de componentes principais para características de crescimento pré-desmame em ovinos da raça Santa Inês

The aim of this study was to evaluate the interrelation among growth traits in Santa Inês lambs using principal component analysis. Data on 270 lambs born between 2008 and 2013 were used. The variables studied were weight at birth, adjusted weights, and average daily gains at 14, 28, 52, and 70 days of age. The wither height, thoracic perimeter, and body length at birth and adjusted at 28 and 70 days of age (weaning), were also used. The statistical model comprised the fixed effects of the contemporary group, the linear effect of julian date at birth, and the linear and quadratic effects of lambing weight. The first principal component explained approximately 89% and 85% of the total variation for body weight at different ages and average daily gains, respectively. The second principal component compared heavier animals or those that gained more weight at 14 days with lighter lambs or those that gained less weight at 14 in the other ages explaining 5% and 10% of the variation, among the animals. The first principal component accounted for 77%, 91%, and 77% of the variation for weight, thoracic perimeter, wither height, and length at birth, 28 days, and 70 days of age, respectively, and compared large animals or animals with greater body volume. The second component, with values of 16%, 6%, and 14% of the total variation at the three ages, respectively, compared animals with distinct conformation to discriminate animals of different shapes and verified that the weights at the different ages and the thoracic perimeter are important sources of variability among the animals.

Objetivou-se com este trabalho avaliar as inter-relações entre as medidas de crescimento em cordeiros da raça Santa Inês no período pré-desmame, utilizando-se a técnica dos componentes principais. Para tanto, contou-se com informações de 270 cordeiros, nascidos entre 2008 e 2013. As variáveis estudadas foram o peso ao nascimento, os pesos corrigidos e ganhos médios diários aos 14, 28, 52 e 70 dias de idade. Foram utilizadas as medidas, altura de cernelha, perímetro torácico e comprimento ao nascimento e corrigidas para os 28 e 70 dias de idade de cordeiros Santa Inês. O modelo estatístico utilizado na análise por componentes principais para todas as características estudadas incluiu os efeitos de grupo de contemporâneos, o efeito linear da data juliana de nascimento e os efeitos linear e quadrático do peso da ovelha ao parto. Para os pesos às diferentes idades e os ganhos médios diários o primeiro componente principal acumulou 89% e 85% da variação total, respectivamente. O segundo componente contrastou animais mais pesados ou ganharam mais peso aos 14 dias com aqueles que foram mais leves ou ganharam menos pesos nas demais idades, explicando 5 e 10% da variabilidade entre os animais. Para as medidas de peso, perímetro torácico, altura de cernelha e comprimento ao nascimento, aos 28 dias de idade e aos 70 dias de idade, o primeiro componente, que acumulou 77%, 91% e 77% da variabilidade existente, respectivamente, comparou animais de maior tamanho ou volume corporal. O segundo componente, com 16%, 6% e 14%, respectivamente da variabilidade total, comparou animais de diferentes conformações e verificou-se que os pesos nas diferentes idades e o perímetro torácico são importantes fontes de variabilidade entre os animais.

Polioencephalomalacia (PEM) in calves associated with excess sulfur intake / Polioencefalomalacia em bezerros associada à ingestão de alimento com excesso de enxofre

Polioencephalomalacia (PEM) is the morphological characterization for softening of brain gray matter, and excess sulfur intake is one of its main causes. This study describes an outbreak of this disease in 1-to-3-month-old calves in a farm located in Santa Catarina state, Brazil. The herd consisted of 27 Jersey male calves whose diet was composed of initial feed, ground whole corn, and mineral salt. From this herd, 10 animals became ill, showing signs of apathy, anorexia and blindness, evolving to generalized weakness and death. Necropsy was performed in three of these animals, which showed flattening of the cerebral convolutions in addition to softened, yellowish areas in the cerebral cortex. Histopathological examination revealed deep laminar necrosis associated with perineuronal and perivascular edema, as well as neurons with wrinkled, eosinophilic, or vacuolated cytoplasm. The following sulfur doses were observed: 8,010mg/kg in corn, 6,385mg/kg in initial feed, 1,060mg/kg in mineral salt and 2.3mg/L in water, reaching dose values far above the accepted, totaling a daily intake of approximately 6,533.5mg sulfur/animal/day. As differential diagnosis, lead was dosed in the kidneys and liver of the three calves, with negative results. Also, the calf that sickened last was treated with 20mg/kg thiamin and 0.2mg/kg dexamethasone (IM; QID) for three days and eventually recovered. According to anatomopathological findings, excess sulfur intake and therapeutic diagnosis, sulfur poisoning was suggested as the cause of PEM in these 1-to-3-month-old calves. Occurrence of PEM is rare in calves at such a young age.(AU)

A polioencefalomalacia (PEM) é a caracterização morfológica para o amolecimento da substância cinzenta encefálica, e uma de suas principais etiologias é a ingestão excessiva de enxofre. Este trabalho descreve um surto desta enfermidade em bezerros de um a três meses de idade em uma propriedade de Santa Catarina. O lote era composto por 27 bezerros machos da raça Jersey, com alimentação composta por ração inicial, milho inteiro triturado e sal mineral. Deste lote, 10 animais adoeceram, apresentando sinais de apatia, anorexia e cegueira, com evolução para fraqueza generalizada. Nove bezerros morreram e três foram submetidos a necropsia, que demonstraram achatamento das circunvoluções cerebrais além de áreas de amolecimento e coloração amarelada no córtex cerebral. Realizou-se exame histopatológico que evidenciou necrose laminar profunda associada a edema perineuronal e perivascular, além de neurônios com citoplasma enrugado, eosinofílico ou vacuolizado. A dosagem de enxofre resultou em 8010mg/Kg no milho, 6385mg/Kg na ração, 1060mg/Kg no sal mineral e 2,3mg/L na água, atingindo valores muito acima do tolerado, totalizando a ingestão diária de cerca de 6533,5mg de enxofre/animal/dia. Como diagnóstico diferencial realizou-se dosagem de chumbo de amostras de rim e fígado dos três bezerros com resultado negativo. Ainda, o último bovino a adoecer foi tratado com 20mg/Kg de tiamina e 0,2mg/Kg de dexametasona IM, QID, durante três dias e recuperou-se. De acordo com os achados anatomopatológicos e o excesso de enxofre na dieta, sugere-se que a intoxicação por enxofre seja a causa de PEM nestes bezerros de um a três meses de idade, sendo essa enfermidade rara em bovinos tão jovens.(AU)

Principal component analysis of pre-weaning growth traits in Santa Inês lambs / Análise de componentes principais para características de crescimento pré-desmame em ovinos da raça Santa Inês

The aim of this study was to evaluate the interrelation among growth traits in Santa Inês lambs using principal component analysis. Data on 270 lambs born between 2008 and 2013 were used. The variables studied were weight at birth, adjusted weights, and average daily gains at 14, 28, 52, and 70 days of age. The wither height, thoracic perimeter, and body length at birth and adjusted at 28 and 70 days of age (weaning), were also used. The statistical model comprised the fixed effects of the contemporary group, the linear effect of julian date at birth, and the linear and quadratic effects of lambing weight. The first principal component explained approximately 89% and 85% of the total variation for body weight at different ages and average daily gains, respectively. The second principal component compared heavier animals or those that gained more weight at 14 days with lighter lambs or those that gained less weight at 14 in the other ages explaining 5% and 10% of the variation, among the animals. The first principal component accounted for 77%, 91%, and 77% of the variation for weight, thoracic perimeter, wither height, and length at birth, 28 days, and 70 days of age, respectively, and compared large animals or animals with greater body volume. The second component, with values of 16%, 6%, and 14% of the total variation at the three ages, respectively, compared animals with distinct conformation to discriminate animals of different shapes and verified that the weights at the different ages and the thoracic perimeter are important sources of variability among the animals.(AU)

Objetivou-se com este trabalho avaliar as inter-relações entre as medidas de crescimento em cordeiros da raça Santa Inês no período pré-desmame, utilizando-se a técnica dos componentes principais. Para tanto, contou-se com informações de 270 cordeiros, nascidos entre 2008 e 2013. As variáveis estudadas foram o peso ao nascimento, os pesos corrigidos e ganhos médios diários aos 14, 28, 52 e 70 dias de idade. Foram utilizadas as medidas, altura de cernelha, perímetro torácico e comprimento ao nascimento e corrigidas para os 28 e 70 dias de idade de cordeiros Santa Inês. O modelo estatístico utilizado na análise por componentes principais para todas as características estudadas incluiu os efeitos de grupo de contemporâneos, o efeito linear da data juliana de nascimento e os efeitos linear e quadrático do peso da ovelha ao parto. Para os pesos às diferentes idades e os ganhos médios diários o primeiro componente principal acumulou 89% e 85% da variação total, respectivamente. O segundo componente contrastou animais mais pesados ou ganharam mais peso aos 14 dias com aqueles que foram mais leves ou ganharam menos pesos nas demais idades, explicando 5 e 10% da variabilidade entre os animais. Para as medidas de peso, perímetro torácico, altura de cernelha e comprimento ao nascimento, aos 28 dias de idade e aos 70 dias de idade, o primeiro componente, que acumulou 77%, 91% e 77% da variabilidade existente, respectivamente, comparou animais de maior tamanho ou volume corporal. O segundo componente, com 16%, 6% e 14%, respectivamente da variabilidade total, comparou animais de diferentes conformações e verificou-se que os pesos nas diferentes idades e o perímetro torácico são importantes fontes de variabilidade entre os animais.(AU)