Planktonic production and respiration in a subtropical lake dominated by Cyanobacteria / Produção e respiração planctônicas em uma lagoa subtropical dominada por cianobactérias

Planktonic primary production and respiration rates were estimated in a subtropical coastal lake dominated by Cyanobacteria in order to investigate the temporal and vertical variation in this lake and to evaluate its relationships with limnological variables and phytoplankton. Light and dark bottles were incubated at four different depths in the central part of the lake and were performed bimonthly from June/2009 to December/2010. No significant difference was evident among depths in relation to phytoplankton, limnological variables and metabolic rates. However, the highest production rates were recorded at the surface, and decreased towards the bottom, coupled with phytoplanktonic photosynthetic capacity. Wind induced mixing in Peri Lake played an important role in nutrient and phytoplankton redistribution, characterizing this lake as polymictic. According to density and biovolume, the phytoplankton community was dominated by filamentous Cyanobacteria, especially Cylindrospermopsis raciborskii (Woloszynska) Seenayya and Subba-Raju. This study has shown that both water temperature and nutrient availability drive phytoplankton growth and consequently the temporal variation in metabolic rates, where respiration is higher than primary production.(AU)

As taxas de produção primária e respiração planctônicas foram estimadas em um lago costeiro subtropical dominado por cianobactérias, com o objetivo de investigar a variação temporal e vertical nesta lagoa e avaliar suas relações com as variáveis limnológicas e os grupos fitoplanctônicos. Garrafas claras e escuras foram incubadas em quatro diferentes profundidades na parte central da lagoa e foram realizadas bimensalmente de junho/2009 a dezembro/2010. Nenhuma diferença significativa foi encontrada entre as profundidades amostradas, em relação ao fitoplâncton, variáveis limnológicas e taxas metabólicas. No entanto, as maiores taxas de produção foram registradas na superfície, diminuindo com o aumento da profundidade, juntamente com a capacidade fotossintética do fitoplâncton. A ação do vento na lagoa do Peri promoveu a homogeneidade de nutrientes e do fitoplâncton na coluna dágua, caracterizando-o como polimítico. De acordo com a densidade e biovolume, a comunidade fitoplanctônica foi dominada por cianobactérias filamentosas, especialmente Cylindrospermopsis raciborskii (Woloszynska) Seenayya and Subba-Raju. Este estudo demonstrou que a temperatura da água combinada com a disponibilidade de nutrientes influencia a comunidade fitoplanctônica, determinando a variação temporal das taxas metabólicas, onde a respiração é maior que a produção primária.(AU)

Yeast diversity associated to sediments and water from two Colombian artificial lakes

In Colombia, knowledge of the yeast and yeast-like fungi community is limited because most studies have focused on species with clinical importance. Sediments and water represent important habitats for the study of yeast diversity, especially for yeast species with industrial, biotechnological, and bioremediation potential. The main purpose of this study was to identify and compare the diversity of yeast species associated with sediment and water samples from two artificial lakes in Universidad del Valle (Cali-Colombia). Yeast samplings were performed from fifteen sediment samples and ten water samples. Grouping of similar isolates was initially based on colony and cell morphology, which was then complemented by micro/mini satellite primed PCR banding pattern analysis by using GTG5 as single primer. A representative isolate for each group established was chosen for D1/D2 domain sequencing and identification. In general, the following yeast species were identified: Candida albicans, Candida diversa, Candida glabrata, Candida pseudolambica, Cryptococcus podzolicus, Cryptococcus rajasthanensis, Cryptococcus laurentii, Williopsis saturnus, Hanseniaspora thailandica, Hanseniaspora uvarum, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Torulaspora delbrueckii, Torulaspora pretoriensis, Tricosporon jirovecii, Trichosporon laibachii and Yarrowia lypolitica. Two possible new species were also found, belonging to the Issatchenkia sp. and Bullera sp. genera. In conclusion, the lakes at the Universidad del Valle campus have significant differences in yeast diversity and species composition between them.(AU)