Resumo
The need for scientific development in goat became larger in order to subsidize farmers withbiotechnology to animal breeding. The advent of frozen semen increased by male progeny, possiblemanipulation and genetic material storage. It seeks to diluents free of animal substances, to ensure food safety inbiological processes. The objective of this research was seminal diluent develop using Mauritia flexuoxa extractas an additive. Obtaining the extract was from dehydrated raw materials, semen collection was performed withartificial vagina and in vitro spermatic evaluation through full force and motility. The extract prepared in TRISdiluent is included at different concentrations. Semen was diluted and evaluated the heat resistance at 37°C. Weevaluated the membrane integrity after 5, 10, 15, 30, 60 and 120 minutes of incubation at 37°C. It wasconcluded that significant differences were observed using Mauritia flexuosa extract.(AU)
Assuntos
Animais , Masculino , Análise do Sêmen/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/química , RuminantesResumo
The need for scientific development in goat became larger in order to subsidize farmers withbiotechnology to animal breeding. The advent of frozen semen increased by male progeny, possiblemanipulation and genetic material storage. It seeks to diluents free of animal substances, to ensure food safety inbiological processes. The objective of this research was seminal diluent develop using Mauritia flexuoxa extractas an additive. Obtaining the extract was from dehydrated raw materials, semen collection was performed withartificial vagina and in vitro spermatic evaluation through full force and motility. The extract prepared in TRISdiluent is included at different concentrations. Semen was diluted and evaluated the heat resistance at 37°C. Weevaluated the membrane integrity after 5, 10, 15, 30, 60 and 120 minutes of incubation at 37°C. It wasconcluded that significant differences were observed using Mauritia flexuosa extract.
Assuntos
Masculino , Animais , Análise do Sêmen/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/química , RuminantesResumo
The aim of this study was to investigate the effects of Tris use supplemented with Mauritia Flexuoxa oilas diluent for goat semen cryopreservation. four goats, clinically healthy were used. The animals were fed dailywith roughage, concentrate and mineral salt will. Initially, we conducted a toxicity test total of 32 collections.After the toxicity test, the concentration was chosen that showed the best result. Soon after, there were 32 morecollections, which were diluted in Tris-egg yolk-glycerol (control group) or GC diluent containing vegetable oil(Mauritia flexuoxa) GB. Samples were cryopreserved with the aid of Tk3000® device. Subsequently sampleswere thawed and analyzed for motility and sperm morphology force. It was observed obtaining post thawmotility in the group plus oil burity which shows a possible alternative to replacement products derived fromanimal origin.(AU)
Assuntos
Animais , Masculino , Criopreservação/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/fisiologia , Análise do Sêmen/métodos , Análise do Sêmen/veterináriaResumo
The aim of this study was to investigate the effects of Tris use supplemented with Mauritia Flexuoxa oilas diluent for goat semen cryopreservation. four goats, clinically healthy were used. The animals were fed dailywith roughage, concentrate and mineral salt will. Initially, we conducted a toxicity test total of 32 collections.After the toxicity test, the concentration was chosen that showed the best result. Soon after, there were 32 morecollections, which were diluted in Tris-egg yolk-glycerol (control group) or GC diluent containing vegetable oil(Mauritia flexuoxa) GB. Samples were cryopreserved with the aid of Tk3000® device. Subsequently sampleswere thawed and analyzed for motility and sperm morphology force. It was observed obtaining post thawmotility in the group plus oil burity which shows a possible alternative to replacement products derived fromanimal origin.